Pub Date : 2021-01-01Epub Date: 2021-06-29DOI: 10.1159/000515068
Santiago Navas-Carretero, Rodrigo San-Cristobal, Ismael Alvarez-Alvarez, Carlos Celis-Morales, Katherine M Livingstone, Claire B O'Donovan, Christina Mavrogianni, Christina P Lambrinou, Yannis Manios, Iwona Traczyck, Christian A Drevon, Cyril F M Marsaux, Wim H M Saris, Rosalind Fallaize, Anna L Macready, Julie A Lovegrove, Thomas E Gundersen, Marianne Walsh, Lorraine Brennan, Eileen R Gibney, Mike Gibney, John C Mathers, J Alfredo Martinez
Introduction: Carbohydrate intake and physical activity are related to glucose homeostasis, both being influenced by individual genetic makeup. However, the interactions between these 2 factors, as affected by genetics, on glycaemia have been scarcely reported.
Objective: We focused on analysing the interplay between carbohydrate intake and physical activity levels on blood glucose, taking into account a genetic risk score (GRS), based on SNPs related to glucose/energy metabolism.
Methods: A total of 1,271 individuals from the Food4Me cohort, who completed the nutritional intervention, were evaluated at baseline. We collected dietary information by using an online-validated food frequency questionnaire, a questionnaire on physical activity, blood biochemistry by analysis of dried blood spots, and by analysis of selected SNPs. Fifteen out of 31 SNPs, with recognized participation in carbohydrate/energy metabolism, were included in the component analyses. The GRS included risk alleles involved in the control of glycaemia or energy-yielding processes.
Results: Data concerning anthropometric, clinical, metabolic, dietary intake, physical activity, and genetics related to blood glucose levels showed expected trends in European individuals of comparable sex and age, being categorized by lifestyle, BMI, and energy/carbohydrate intakes, in this Food4Me population. Blood glucose was inversely associated with physical activity level (β = -0.041, p = 0.013) and positively correlated with the GRS values (β = 0.015, p = 0.047). Interestingly, an interaction affecting glycaemia, concerning physical activity level with carbohydrate intake, was found (β = -0.060, p = 0.033), which also significantly depended on the genetic background (GRS).
Conclusions: The relationships of carbohydrate intake and physical activity are important in understanding glucose homeostasis, where a role for the genetic background should be ascribed.
碳水化合物的摄入和身体活动与葡萄糖稳态有关,两者都受到个体基因组成的影响。然而,这两个因素之间的相互作用,受遗传影响,对血糖几乎没有报道。目的:考虑到与葡萄糖/能量代谢相关的snp的遗传风险评分(GRS),我们重点分析了碳水化合物摄入量和身体活动水平对血糖的相互作用。方法:来自Food4Me队列的1271名完成营养干预的个体在基线时进行评估。我们通过在线验证的食物频率问卷、身体活动问卷、通过分析干血斑和选择的snp分析来收集饮食信息。31个snp中有15个被认为参与碳水化合物/能量代谢,被纳入成分分析。GRS包括参与控制血糖或能量产生过程的风险等位基因。结果:在Food4Me人群中,与血糖水平相关的人体测量学、临床、代谢、饮食摄入、身体活动和遗传学数据显示,在性别和年龄相仿的欧洲个体中,按生活方式、BMI和能量/碳水化合物摄入量进行分类的趋势是预期的。血糖与体力活动水平呈负相关(β = -0.041, p = 0.013),与GRS值呈正相关(β = 0.015, p = 0.047)。有趣的是,研究发现身体活动水平与碳水化合物摄入量之间存在影响血糖的相互作用(β = -0.060, p = 0.033),这也显著依赖于遗传背景(GRS)。结论:碳水化合物摄入和身体活动的关系对于理解葡萄糖稳态是重要的,其中遗传背景的作用应归因于。
{"title":"Interactions of Carbohydrate Intake and Physical Activity with Regulatory Genes Affecting Glycaemia: A Food4Me Study Analysis.","authors":"Santiago Navas-Carretero, Rodrigo San-Cristobal, Ismael Alvarez-Alvarez, Carlos Celis-Morales, Katherine M Livingstone, Claire B O'Donovan, Christina Mavrogianni, Christina P Lambrinou, Yannis Manios, Iwona Traczyck, Christian A Drevon, Cyril F M Marsaux, Wim H M Saris, Rosalind Fallaize, Anna L Macready, Julie A Lovegrove, Thomas E Gundersen, Marianne Walsh, Lorraine Brennan, Eileen R Gibney, Mike Gibney, John C Mathers, J Alfredo Martinez","doi":"10.1159/000515068","DOIUrl":"https://doi.org/10.1159/000515068","url":null,"abstract":"<p><strong>Introduction: </strong>Carbohydrate intake and physical activity are related to glucose homeostasis, both being influenced by individual genetic makeup. However, the interactions between these 2 factors, as affected by genetics, on glycaemia have been scarcely reported.</p><p><strong>Objective: </strong>We focused on analysing the interplay between carbohydrate intake and physical activity levels on blood glucose, taking into account a genetic risk score (GRS), based on SNPs related to glucose/energy metabolism.</p><p><strong>Methods: </strong>A total of 1,271 individuals from the Food4Me cohort, who completed the nutritional intervention, were evaluated at baseline. We collected dietary information by using an online-validated food frequency questionnaire, a questionnaire on physical activity, blood biochemistry by analysis of dried blood spots, and by analysis of selected SNPs. Fifteen out of 31 SNPs, with recognized participation in carbohydrate/energy metabolism, were included in the component analyses. The GRS included risk alleles involved in the control of glycaemia or energy-yielding processes.</p><p><strong>Results: </strong>Data concerning anthropometric, clinical, metabolic, dietary intake, physical activity, and genetics related to blood glucose levels showed expected trends in European individuals of comparable sex and age, being categorized by lifestyle, BMI, and energy/carbohydrate intakes, in this Food4Me population. Blood glucose was inversely associated with physical activity level (β = -0.041, p = 0.013) and positively correlated with the GRS values (β = 0.015, p = 0.047). Interestingly, an interaction affecting glycaemia, concerning physical activity level with carbohydrate intake, was found (β = -0.060, p = 0.033), which also significantly depended on the genetic background (GRS).</p><p><strong>Conclusions: </strong>The relationships of carbohydrate intake and physical activity are important in understanding glucose homeostasis, where a role for the genetic background should be ascribed.</p>","PeriodicalId":18030,"journal":{"name":"Lifestyle Genomics","volume":"14 3","pages":"63-72"},"PeriodicalIF":2.6,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000515068","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39119289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01Epub Date: 2020-10-01DOI: 10.1159/000510216
Justine R Horne, Jason Gilliland, Tara Leckie, Colleen O'Connor, Jamie A Seabrook, Janet Madill
Background: Lifestyle genomics (LGx) is a science that explores interactions between genetic variation, lifestyle components such as physical activity (PA), and subsequent health- and performance-related outcomes. The objective of this study was to determine whether an LGx intervention could motivate enhanced engagement in PA to a greater extent than a population-based intervention.
Methods: In this pragmatic randomized controlled trial, participants received either the standard, population-based Group Lifestyle BalanceTM (GLB) program intervention or the GLB program in addition to the provision of LGx information and advice (GLB + LGx). Participants (n = 140) completed a 7-day PA recall at baseline, 3, 6, and 12 months. Data from the PA recalls were used to calculate metabolic equivalents (METs), a measure of energy expenditure. Statistical analyses included split plot analyses of covariance and binary logistic regression (generalized linear models). Differences in leisure time PA weekly METs, weekly minutes of moderate + high-intensity PA, and adherence to PA guidelines were compared between groups (GLB and GLB + LGx) across the 4 time points.
Results: Weekly METs were significantly higher in the GLB + LGx group (1,114.7 ± 141.9; 95% CI 831.5-1,397.8) compared to the standard GLB group (621.6 ± 141.9 MET/week; 95% CI 338.4-904.8) at the 6-month follow-up (p = 0.01). All other results were non-significant.
Conclusions: The provision of an LGx intervention resulted in a greater weekly leisure time PA energy expenditure after the 6-month follow-up. Future research should determine how this could be sustained over the long-term.
Clinical trial registration: NCT03015012.
背景:生活方式基因组学(LGx)是一门探索遗传变异、生活方式成分(如体力活动(PA))以及随后的健康和表现相关结果之间相互作用的科学。本研究的目的是确定LGx干预是否能比基于人群的干预更大程度地促进PA的参与。方法:在这个实用的随机对照试验中,参与者接受了标准的、基于人群的群体生活方式平衡(GLB)计划干预,或者GLB计划除了提供LGx信息和建议(GLB + LGx)。参与者(n = 140)在基线、3、6和12个月完成了为期7天的PA回忆。来自PA召回的数据用于计算代谢当量(METs),这是一种能量消耗的测量方法。统计分析包括协方差的分割图分析和二元逻辑回归(广义线性模型)。在4个时间点上比较各组(GLB组和GLB + LGx组)之间的休闲时间PA每周met、每周中+高强度PA分钟数和对PA指南的依从性的差异。结果:GLB + LGx组每周met显著高于对照组(1,114.7±141.9;95% CI 831.5- 1397.8)与标准GLB组(621.6±141.9 MET/周;95% CI 338.4-904.8),随访6个月(p = 0.01)。其他结果均无统计学意义。结论:在6个月的随访后,提供LGx干预导致每周休闲时间PA能量消耗增加。未来的研究应该确定这种情况如何才能长期维持下去。临床试验注册:NCT03015012。
{"title":"Can a Lifestyle Genomics Intervention Motivate Patients to Engage in Greater Physical Activity than a Population-Based Intervention? Results from the NOW Randomized Controlled Trial.","authors":"Justine R Horne, Jason Gilliland, Tara Leckie, Colleen O'Connor, Jamie A Seabrook, Janet Madill","doi":"10.1159/000510216","DOIUrl":"https://doi.org/10.1159/000510216","url":null,"abstract":"<p><strong>Background: </strong>Lifestyle genomics (LGx) is a science that explores interactions between genetic variation, lifestyle components such as physical activity (PA), and subsequent health- and performance-related outcomes. The objective of this study was to determine whether an LGx intervention could motivate enhanced engagement in PA to a greater extent than a population-based intervention.</p><p><strong>Methods: </strong>In this pragmatic randomized controlled trial, participants received either the standard, population-based Group Lifestyle BalanceTM (GLB) program intervention or the GLB program in addition to the provision of LGx information and advice (GLB + LGx). Participants (n = 140) completed a 7-day PA recall at baseline, 3, 6, and 12 months. Data from the PA recalls were used to calculate metabolic equivalents (METs), a measure of energy expenditure. Statistical analyses included split plot analyses of covariance and binary logistic regression (generalized linear models). Differences in leisure time PA weekly METs, weekly minutes of moderate + high-intensity PA, and adherence to PA guidelines were compared between groups (GLB and GLB + LGx) across the 4 time points.</p><p><strong>Results: </strong>Weekly METs were significantly higher in the GLB + LGx group (1,114.7 ± 141.9; 95% CI 831.5-1,397.8) compared to the standard GLB group (621.6 ± 141.9 MET/week; 95% CI 338.4-904.8) at the 6-month follow-up (p = 0.01). All other results were non-significant.</p><p><strong>Conclusions: </strong>The provision of an LGx intervention resulted in a greater weekly leisure time PA energy expenditure after the 6-month follow-up. Future research should determine how this could be sustained over the long-term.</p><p><strong>Clinical trial registration: </strong>NCT03015012.</p>","PeriodicalId":18030,"journal":{"name":"Lifestyle Genomics","volume":"13 6","pages":"180-186"},"PeriodicalIF":2.6,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000510216","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38442647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01Epub Date: 2020-02-26DOI: 10.1159/000505662
Pui Yee Tan, Soma Roy Mitra
Background: Computing polygenic risk scores (PRS) to predict the degree of risk for obesity may contribute to weight management programs strategically.
Objectives: To investigate the combined effect of FTO rs9930501, rs9930506, and rs9932754 and ADRB2 rs1042713 and rs1042714 using PRS on (1) the odds of obesity and (2) post-intervention differences in dietary, anthropometric, and cardiometabolic parameters in response to high-protein calorie-restricted, high-vitamin E, high-fiber (Hipcref) diet intervention in Malaysian adults.
Methods: Both a cross-sectional study (n = 178) and a randomized controlled trial (RCT) (n = 128) were conducted to test the aforementioned objectives. PRS was computed as the weighted sum of the risk alleles possessed by each individual participant. Participants were stratified into first (PRS 0-0.64), second (PRS 0.65-3.59), and third (PRS 3.60-8.18) tertiles.
Results: The third tertile of PRS was associated with significantly higher odds of obesity: 2.29 (95% CI = 1.11-4.72, adjusted p = 0.025) compared to the first tertile. Indians (3.9 ± 0.3) had significantly higher PRS compared to Chinese (2.1 ± 0.4) (p = 0.010). In the RCT, a greater reduction in high-sensitivity C-reactive protein (hsCRP) levels was found in second and third tertiles after Hipcref diet intervention compared to the control diet (p interaction = 0.048).
Conclusion: Higher PRS was significantly associated with increased odds of obesity. Individuals with higher PRS had a significantly greater reduction in hsCRP levels after Hipcref diet compared to the control diet.
{"title":"The Combined Effect of Polygenic Risk from FTO and ADRB2 Gene Variants, Odds of Obesity, and Post-Hipcref Diet Differences.","authors":"Pui Yee Tan, Soma Roy Mitra","doi":"10.1159/000505662","DOIUrl":"https://doi.org/10.1159/000505662","url":null,"abstract":"<p><strong>Background: </strong>Computing polygenic risk scores (PRS) to predict the degree of risk for obesity may contribute to weight management programs strategically.</p><p><strong>Objectives: </strong>To investigate the combined effect of FTO rs9930501, rs9930506, and rs9932754 and ADRB2 rs1042713 and rs1042714 using PRS on (1) the odds of obesity and (2) post-intervention differences in dietary, anthropometric, and cardiometabolic parameters in response to high-protein calorie-restricted, high-vitamin E, high-fiber (Hipcref) diet intervention in Malaysian adults.</p><p><strong>Methods: </strong>Both a cross-sectional study (n = 178) and a randomized controlled trial (RCT) (n = 128) were conducted to test the aforementioned objectives. PRS was computed as the weighted sum of the risk alleles possessed by each individual participant. Participants were stratified into first (PRS 0-0.64), second (PRS 0.65-3.59), and third (PRS 3.60-8.18) tertiles.</p><p><strong>Results: </strong>The third tertile of PRS was associated with significantly higher odds of obesity: 2.29 (95% CI = 1.11-4.72, adjusted p = 0.025) compared to the first tertile. Indians (3.9 ± 0.3) had significantly higher PRS compared to Chinese (2.1 ± 0.4) (p = 0.010). In the RCT, a greater reduction in high-sensitivity C-reactive protein (hsCRP) levels was found in second and third tertiles after Hipcref diet intervention compared to the control diet (p interaction = 0.048).</p><p><strong>Conclusion: </strong>Higher PRS was significantly associated with increased odds of obesity. Individuals with higher PRS had a significantly greater reduction in hsCRP levels after Hipcref diet compared to the control diet.</p>","PeriodicalId":18030,"journal":{"name":"Lifestyle Genomics","volume":"13 2","pages":"84-98"},"PeriodicalIF":2.6,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000505662","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37679270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01Epub Date: 2020-10-19DOI: 10.1159/000508819
Daniel A de Luis, David Primo, Olatz Izaola, Emilia Gómez, Rosario Bachiller
Background: The role of adiponectin (ADIPOQ) polymorphisms in weight loss and serum lipid changes following different dietary interventions remain unclear. The Mediterranean dietary pattern has been associated with improved cardiovascular risk factors in different studies.
Objective: Our aim was to analyze the effects of a hypocaloric diet with a Mediterranean dietary pattern on the metabolic response and adiposity parameters, taking into account the 712 G/A rs3774261 polymorphisms in ADIPOQ.
Design: A population of 135 obese patients was enrolled. Anthropometric and serum parameters (lipid profile, insulin, homeostasis model assessment for insulin resistance [HOMA-IR], glucose, C-reactive protein [CRP], adiponectin, resistin, and leptin levels) were measured before and after the dietary intervention (12 weeks). All of the patients were genotyped for the rs3774261 polymorphism.
Results: The genotype distribution of this population was 36 patients with AA (26.7%), 68 patients with AG (50.4%), and 31 patients with GG (22.9%). After the dietary intervention and in both genotypes, BMI, weight, fat mass, systolic blood pressure, waist circumference, glucose, insulin, HOMA-IR, and leptin levels all decreased. After the dietary intervention with secondary weight loss and in non-G-allele carriers (AA vs. AG+GG), total cholesterol (Δ = -15.7 ± 3.9 vs. -4.9 ± 2.9 mg/dL; p = 0.02), LDL cholesterol (Δ = -15.3 ± 3.8 vs. -1.7 ± 1.9 mg/dL; p = 0.01), triglyceride levels (Δ = -23.4 ± 5.6 vs. 2.3 ± 2.3 mg/dL; p = 0.01), and CRP (Δ = -1.1 ± 0.1 vs. -0.4 ± 0.2 mg/dL; p = 0.01) decreased. Adiponectin levels (Δ = 7.2 ± 2.1 vs. -0.4 ± 0.3 ng/dL; p = 0.02) increased. Notably, G-allele carriers did not show this improvement.
Conclusion: Non-G-allele carriers of the ADIPOQ variant (rs3774261) showed significant improvement in serum levels of adiponectin, lipid profiles, and CRP in response to a hypocaloric diet with a Mediterranean dietary pattern.
背景:脂联素(ADIPOQ)多态性在不同饮食干预后体重减轻和血脂变化中的作用尚不清楚。在不同的研究中,地中海饮食模式与改善心血管风险因素有关。目的:我们的目的是分析低热量饮食和地中海饮食模式对代谢反应和肥胖参数的影响,同时考虑到ADIPOQ的712 G/ a rs3774261多态性。设计:纳入135例肥胖患者。在饮食干预前后(12周)测量人体测量和血清参数(血脂、胰岛素、胰岛素抵抗稳态模型评估[HOMA-IR]、葡萄糖、c -反应蛋白[CRP]、脂联素、抵抗素和瘦素水平)。所有患者均进行rs3774261多态性基因分型。结果:AA型36例(26.7%),AG型68例(50.4%),GG型31例(22.9%)。在饮食干预后,两种基因型的BMI、体重、脂肪量、收缩压、腰围、葡萄糖、胰岛素、HOMA-IR和瘦素水平均下降。饮食干预后继发性体重减轻和非g等位基因携带者(AA vs AG+GG),总胆固醇(Δ = -15.7±3.9 vs -4.9±2.9 mg/dL;p = 0.02), LDL胆固醇(Δ = -15.3±3.8 vs. -1.7±1.9 mg/dL;p = 0.01),甘油三酯水平(Δ = -23.4±5.6 vs. 2.3±2.3 mg/dL;p = 0.01)和c反应蛋白(Δ= -1.1±0.1和-0.4±0.2 mg / dL;P = 0.01)降低。脂联素水平(Δ = 7.2±2.1 vs. -0.4±0.3 ng/dL;P = 0.02)升高。值得注意的是,g等位基因携带者没有表现出这种改善。结论:ADIPOQ变体(rs3774261)的非g等位基因携带者在低热量饮食和地中海饮食模式下,血清脂联素水平、脂质谱和CRP水平显著改善。
{"title":"Serum Lipid and Adiponectin Improvements after a Mediterranean Dietary Pattern in Non-G-Allele Carriers of the Variant rs3774261.","authors":"Daniel A de Luis, David Primo, Olatz Izaola, Emilia Gómez, Rosario Bachiller","doi":"10.1159/000508819","DOIUrl":"https://doi.org/10.1159/000508819","url":null,"abstract":"<p><strong>Background: </strong>The role of adiponectin (ADIPOQ) polymorphisms in weight loss and serum lipid changes following different dietary interventions remain unclear. The Mediterranean dietary pattern has been associated with improved cardiovascular risk factors in different studies.</p><p><strong>Objective: </strong>Our aim was to analyze the effects of a hypocaloric diet with a Mediterranean dietary pattern on the metabolic response and adiposity parameters, taking into account the 712 G/A rs3774261 polymorphisms in ADIPOQ.</p><p><strong>Design: </strong>A population of 135 obese patients was enrolled. Anthropometric and serum parameters (lipid profile, insulin, homeostasis model assessment for insulin resistance [HOMA-IR], glucose, C-reactive protein [CRP], adiponectin, resistin, and leptin levels) were measured before and after the dietary intervention (12 weeks). All of the patients were genotyped for the rs3774261 polymorphism.</p><p><strong>Results: </strong>The genotype distribution of this population was 36 patients with AA (26.7%), 68 patients with AG (50.4%), and 31 patients with GG (22.9%). After the dietary intervention and in both genotypes, BMI, weight, fat mass, systolic blood pressure, waist circumference, glucose, insulin, HOMA-IR, and leptin levels all decreased. After the dietary intervention with secondary weight loss and in non-G-allele carriers (AA vs. AG+GG), total cholesterol (Δ = -15.7 ± 3.9 vs. -4.9 ± 2.9 mg/dL; p = 0.02), LDL cholesterol (Δ = -15.3 ± 3.8 vs. -1.7 ± 1.9 mg/dL; p = 0.01), triglyceride levels (Δ = -23.4 ± 5.6 vs. 2.3 ± 2.3 mg/dL; p = 0.01), and CRP (Δ = -1.1 ± 0.1 vs. -0.4 ± 0.2 mg/dL; p = 0.01) decreased. Adiponectin levels (Δ = 7.2 ± 2.1 vs. -0.4 ± 0.3 ng/dL; p = 0.02) increased. Notably, G-allele carriers did not show this improvement.</p><p><strong>Conclusion: </strong>Non-G-allele carriers of the ADIPOQ variant (rs3774261) showed significant improvement in serum levels of adiponectin, lipid profiles, and CRP in response to a hypocaloric diet with a Mediterranean dietary pattern.</p>","PeriodicalId":18030,"journal":{"name":"Lifestyle Genomics","volume":"13 6","pages":"164-171"},"PeriodicalIF":2.6,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000508819","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38601134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Even though excessive adipose tissue is related to chronic metabolic disturbances, not all subjects with excess weight (EW) display metabolic alterations, and not all normal-weight (NW) subjects have a metabolically healthy (MH) phenotype, probably due to gene-environment interactions. The aim of this study was to investigate the interaction effects of ADIPOQ and PPARG genetic variants in NW and EW individuals with different metabolic phenotypes.
Methods: Data on 345 adults from western Mexico were analyzed. The individuals were classified into NW and EW groups according to body mass index, and were categorized as MH or metabolically unhealthy (MUH), considering homeostatic model assessment insulin resistance (HOMA-IR) and National Cholesterol Education Program Adult Treatment Panel III (NCEP-ATP III) cut-off points for glucose, triglycerides, high-density lipoprotein cholesterol, and blood pressure. Subjects with ≤1 altered parameter were classified as MH. The single nucleotide polymorphisms (SNPs) -11377C>G, -11391G>A, +45T>G, and +276G>T for ADIPOQ and Pro12Ala for PPARG were analyzed by allelic discrimination. High-molecular-weight adiponectin isoform levels were measured by ELISA.
Results: Lower serum adiponectin levels were associated with the MUH phenotype in EW subjects. NW subjects with the GG or TG genotype for the +45T>G SNP had reduced odds of the MUH phenotype. Individuals who carried two copies of the GG haplotype at the -11391G>A and -11377C>G SNPs for ADIPOQ had lower serum adiponectin levels than those with zero copies.
Conclusion: In this population, lower serum adiponectin levels were found in the EW-MUH phenotype, and no differences were observed between the NW-MH and the EW-MH phenotype. In addition, the +45T>G SNP was associated with reduced odds of the MUH phenotype.
{"title":"A Metabolically Unhealthy Phenotype Is Associated with ADIPOQ Genetic Variants and Lower Serum Adiponectin Levels.","authors":"Nathaly Torres-Castillo, Wendy Campos-Perez, Roberto Rodriguez-Echevarria, Sarai Citlalic Rodriguez-Reyes, Erika Martinez-Lopez","doi":"10.1159/000510021","DOIUrl":"https://doi.org/10.1159/000510021","url":null,"abstract":"<p><strong>Background: </strong>Even though excessive adipose tissue is related to chronic metabolic disturbances, not all subjects with excess weight (EW) display metabolic alterations, and not all normal-weight (NW) subjects have a metabolically healthy (MH) phenotype, probably due to gene-environment interactions. The aim of this study was to investigate the interaction effects of ADIPOQ and PPARG genetic variants in NW and EW individuals with different metabolic phenotypes.</p><p><strong>Methods: </strong>Data on 345 adults from western Mexico were analyzed. The individuals were classified into NW and EW groups according to body mass index, and were categorized as MH or metabolically unhealthy (MUH), considering homeostatic model assessment insulin resistance (HOMA-IR) and National Cholesterol Education Program Adult Treatment Panel III (NCEP-ATP III) cut-off points for glucose, triglycerides, high-density lipoprotein cholesterol, and blood pressure. Subjects with ≤1 altered parameter were classified as MH. The single nucleotide polymorphisms (SNPs) -11377C>G, -11391G>A, +45T>G, and +276G>T for ADIPOQ and Pro12Ala for PPARG were analyzed by allelic discrimination. High-molecular-weight adiponectin isoform levels were measured by ELISA.</p><p><strong>Results: </strong>Lower serum adiponectin levels were associated with the MUH phenotype in EW subjects. NW subjects with the GG or TG genotype for the +45T>G SNP had reduced odds of the MUH phenotype. Individuals who carried two copies of the GG haplotype at the -11391G>A and -11377C>G SNPs for ADIPOQ had lower serum adiponectin levels than those with zero copies.</p><p><strong>Conclusion: </strong>In this population, lower serum adiponectin levels were found in the EW-MUH phenotype, and no differences were observed between the NW-MH and the EW-MH phenotype. In addition, the +45T>G SNP was associated with reduced odds of the MUH phenotype.</p>","PeriodicalId":18030,"journal":{"name":"Lifestyle Genomics","volume":"13 6","pages":"172-179"},"PeriodicalIF":2.6,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000510021","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38560970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01Epub Date: 2020-08-07DOI: 10.1159/000510217
David M Mutch
[...]these natural experiments happen whether people want them to or not! Even after strict travel restrictions were implemented, the virus spread rapidly across the USA and phylogenetic analyses have proved useful to clarify the importance of domestic transmission [5]. [...]recent bioinformatic analyses of complete viral genomes suggest increasing diversification of SARS-CoV-2 strains [6]. Furthermore, a study in patients with lupus showed that the ACE2 gene is under epigenetic control, where hypomethylation was associated with increased ACE2 gene expression [10]. [...]it is plausible that lifestyle factors that regulate the epigenome may also alter a person’s risk of infection. [...]it will be important to follow babies born at different points during the pandemic, as well as young children, to determine whether COVID-19-induced lifestyle changes in parents have any long-lasting ramifications on offspring as they age.
{"title":"The Covid-19 Global Pandemic: A Natural Experiment in the Making.","authors":"David M Mutch","doi":"10.1159/000510217","DOIUrl":"https://doi.org/10.1159/000510217","url":null,"abstract":"[...]these natural experiments happen whether people want them to or not! Even after strict travel restrictions were implemented, the virus spread rapidly across the USA and phylogenetic analyses have proved useful to clarify the importance of domestic transmission [5]. [...]recent bioinformatic analyses of complete viral genomes suggest increasing diversification of SARS-CoV-2 strains [6]. Furthermore, a study in patients with lupus showed that the ACE2 gene is under epigenetic control, where hypomethylation was associated with increased ACE2 gene expression [10]. [...]it is plausible that lifestyle factors that regulate the epigenome may also alter a person’s risk of infection. [...]it will be important to follow babies born at different points during the pandemic, as well as young children, to determine whether COVID-19-induced lifestyle changes in parents have any long-lasting ramifications on offspring as they age.","PeriodicalId":18030,"journal":{"name":"Lifestyle Genomics","volume":"13 5","pages":"135-137"},"PeriodicalIF":2.6,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000510217","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38244330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01Epub Date: 2020-05-05DOI: 10.1159/000507252
Yael Joffe, Heléne Herholdt
Background: The past two decades have seen exponential growth in the number of genetic testing companies, but only a small percentage of these tests are being sold through health care professionals (HCPs). As each new genetic testing company appears, it is becoming more difficult for the practitioner and consumer to evaluate the credibility of the claims being made and the value of the tests being offered.
Summary: HCPs appear to have minimal nutrigenomics knowledge and little confidence in choosing and interpreting nutrigenetic tests. To remedy this, HCPs need access to credible education, professional support, networking, career development, mentorship, and a regulated testing environment. This will enable them to evaluate the credibility of genetic tests and testing companies, provide genetic results in context, and apply appropriate clinical translation. Key Message: In order to establish an expert group of nutrigenomic practitioners, collaboration is required between educational institutions, professional organizations, and genetic testing companies. This will provide the necessary support, skills, and knowledge to ensure that the best value is extracted from nutrigenetic tests in an ethical and responsible manner.
{"title":"What Will It Take to Build an Expert Group of Nutrigenomic Practitioners?","authors":"Yael Joffe, Heléne Herholdt","doi":"10.1159/000507252","DOIUrl":"https://doi.org/10.1159/000507252","url":null,"abstract":"<p><strong>Background: </strong>The past two decades have seen exponential growth in the number of genetic testing companies, but only a small percentage of these tests are being sold through health care professionals (HCPs). As each new genetic testing company appears, it is becoming more difficult for the practitioner and consumer to evaluate the credibility of the claims being made and the value of the tests being offered.</p><p><strong>Summary: </strong>HCPs appear to have minimal nutrigenomics knowledge and little confidence in choosing and interpreting nutrigenetic tests. To remedy this, HCPs need access to credible education, professional support, networking, career development, mentorship, and a regulated testing environment. This will enable them to evaluate the credibility of genetic tests and testing companies, provide genetic results in context, and apply appropriate clinical translation. Key Message: In order to establish an expert group of nutrigenomic practitioners, collaboration is required between educational institutions, professional organizations, and genetic testing companies. This will provide the necessary support, skills, and knowledge to ensure that the best value is extracted from nutrigenetic tests in an ethical and responsible manner.</p>","PeriodicalId":18030,"journal":{"name":"Lifestyle Genomics","volume":"13 3","pages":"122-128"},"PeriodicalIF":2.6,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000507252","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37901496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01Epub Date: 2020-05-06DOI: 10.1159/000506769
Michael A Schmidt, Cem Meydan, Caleb M Schmidt, Ebrahim Afshinnekoo, Christopher E Mason
Background: At present, there is no clear understanding of the effect of long-duration spaceflight on the major enzymes that govern the metabolism of omega-6 and omega-3 fatty acids. To address this gap in knowledge, we used data from the NASA Twins Study, which includes a multiscale omics investigation of the changes that occurred during a year-long (340 days) human spaceflight. Embedded within the NASA Twins data are specific analytes associated with fatty acid metabolism.
Objectives: To examine the long-chain fatty acid desaturases and elongases in a single human during 1 year in space.
Method: One male twin was on board the International Space Station (ISS) for 1 year, while his monozygotic twin served as a genetically matched ground control. Longitudinal assessments included the genome, epige-nome, transcriptome, proteome, metabolome, microbiome, and immunome during the mission, as well as 6 months before and after. The gene-specific fatty acid desaturase and elongase transcriptome data (FADS1, FADS2, ELOVL2, and ELOVL5) were extracted from untargeted RNA-seq measurements derived from white blood cell fractions.
Results: Most data from the elongases and desaturases exhibited relatively similar expression profiles (R2 >0.6) over time for the CD8, CD19, and lymphocyte-depleted (LD) cell fractions, indicating overall conservation of function within and between the subjects. Both cell-type and temporal specificity was observed in some cases, and some differences were also apparent between the polyadenylated (polyA) fraction of processed RNAs versus the ribodepleted (ribo-) fraction. The flight subject showed a stronger enrichment of the fatty acid metabolic process pathway across almost all cell types (columns, CD4, CD8, CPT, and LD), most especially in the ribodepleted fraction of RNA, but also with the polyA+ fraction of RNA. Gene set enrichment analysis (GSEA) measures across three related fatty acid metabolism pathways showed a differential between the ground and the flight subject.
Conclusions: There appears to be no persistent alteration of desaturase and elongase gene expression associated with 1 year in space. However, these data provide evidence that cellular lipid metabolism can be responsive and dynamic to spaceflight, even though it appears cell-type and context specific, most notably in terms of the fraction of RNA measured and the collection protocols. These results also provide new evidence of mid-flight spikes in expression of selected genes, which may indicate transient responses to specific insults during spaceflight.
{"title":"The NASA Twins Study: The Effect of One Year in Space on Long-Chain Fatty Acid Desaturases and Elongases.","authors":"Michael A Schmidt, Cem Meydan, Caleb M Schmidt, Ebrahim Afshinnekoo, Christopher E Mason","doi":"10.1159/000506769","DOIUrl":"10.1159/000506769","url":null,"abstract":"<p><strong>Background: </strong>At present, there is no clear understanding of the effect of long-duration spaceflight on the major enzymes that govern the metabolism of omega-6 and omega-3 fatty acids. To address this gap in knowledge, we used data from the NASA Twins Study, which includes a multiscale omics investigation of the changes that occurred during a year-long (340 days) human spaceflight. Embedded within the NASA Twins data are specific analytes associated with fatty acid metabolism.</p><p><strong>Objectives: </strong>To examine the long-chain fatty acid desaturases and elongases in a single human during 1 year in space.</p><p><strong>Method: </strong>One male twin was on board the International Space Station (ISS) for 1 year, while his monozygotic twin served as a genetically matched ground control. Longitudinal assessments included the genome, epige-nome, transcriptome, proteome, metabolome, microbiome, and immunome during the mission, as well as 6 months before and after. The gene-specific fatty acid desaturase and elongase transcriptome data (FADS1, FADS2, ELOVL2, and ELOVL5) were extracted from untargeted RNA-seq measurements derived from white blood cell fractions.</p><p><strong>Results: </strong>Most data from the elongases and desaturases exhibited relatively similar expression profiles (R2 >0.6) over time for the CD8, CD19, and lymphocyte-depleted (LD) cell fractions, indicating overall conservation of function within and between the subjects. Both cell-type and temporal specificity was observed in some cases, and some differences were also apparent between the polyadenylated (polyA) fraction of processed RNAs versus the ribodepleted (ribo-) fraction. The flight subject showed a stronger enrichment of the fatty acid metabolic process pathway across almost all cell types (columns, CD4, CD8, CPT, and LD), most especially in the ribodepleted fraction of RNA, but also with the polyA+ fraction of RNA. Gene set enrichment analysis (GSEA) measures across three related fatty acid metabolism pathways showed a differential between the ground and the flight subject.</p><p><strong>Conclusions: </strong>There appears to be no persistent alteration of desaturase and elongase gene expression associated with 1 year in space. However, these data provide evidence that cellular lipid metabolism can be responsive and dynamic to spaceflight, even though it appears cell-type and context specific, most notably in terms of the fraction of RNA measured and the collection protocols. These results also provide new evidence of mid-flight spikes in expression of selected genes, which may indicate transient responses to specific insults during spaceflight.</p>","PeriodicalId":18030,"journal":{"name":"Lifestyle Genomics","volume":"13 3","pages":"107-121"},"PeriodicalIF":2.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37906773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01Epub Date: 2019-12-10DOI: 10.1159/000503996
Michael T Paris
Background: Body composition is increasingly being recognized as an important prognostic factor for health outcomes across cancer, liver cirrhosis, and critically ill patients. Computed tomography (CT) scans, when taken as part of routine care, provide an excellent opportunity to precisely measure the quantity and quality of skeletal muscle and adipose tissue. However, manual analysis of CT scans is costly and time-intensive, limiting the widespread adoption of CT-based measurements of body composition.
Summary: Advances in deep learning have demonstrated excellent success in biomedical image analysis. Several recent publications have demonstrated excellent accuracy in comparison to human raters for the measurement of skeletal muscle, visceral adipose, and subcutaneous adipose tissue from the lumbar vertebrae region, indicating that analysis of body composition may be successfully automated using deep neural networks. Key Messages: The high accuracy and drastically improved speed of CT body composition analysis (<1 s/scan for neural networks vs. 15 min/scan for human analysis) suggest that neural networks may aid researchers and clinicians in better understanding the role of body composition in clinical populations by enabling cost-effective, large-scale research studies. As the role of body composition in clinical settings and the field of automated analysis advance, it will be critical to examine how clinicians interact with these systems and to evaluate whether these technologies are beneficial in improving treatment and health outcomes for patients.
{"title":"Body Composition Analysis of Computed Tomography Scans in Clinical Populations: The Role of Deep Learning.","authors":"Michael T Paris","doi":"10.1159/000503996","DOIUrl":"https://doi.org/10.1159/000503996","url":null,"abstract":"<p><strong>Background: </strong>Body composition is increasingly being recognized as an important prognostic factor for health outcomes across cancer, liver cirrhosis, and critically ill patients. Computed tomography (CT) scans, when taken as part of routine care, provide an excellent opportunity to precisely measure the quantity and quality of skeletal muscle and adipose tissue. However, manual analysis of CT scans is costly and time-intensive, limiting the widespread adoption of CT-based measurements of body composition.</p><p><strong>Summary: </strong>Advances in deep learning have demonstrated excellent success in biomedical image analysis. Several recent publications have demonstrated excellent accuracy in comparison to human raters for the measurement of skeletal muscle, visceral adipose, and subcutaneous adipose tissue from the lumbar vertebrae region, indicating that analysis of body composition may be successfully automated using deep neural networks. Key Messages: The high accuracy and drastically improved speed of CT body composition analysis (<1 s/scan for neural networks vs. 15 min/scan for human analysis) suggest that neural networks may aid researchers and clinicians in better understanding the role of body composition in clinical populations by enabling cost-effective, large-scale research studies. As the role of body composition in clinical settings and the field of automated analysis advance, it will be critical to examine how clinicians interact with these systems and to evaluate whether these technologies are beneficial in improving treatment and health outcomes for patients.</p>","PeriodicalId":18030,"journal":{"name":"Lifestyle Genomics","volume":"13 1","pages":"28-31"},"PeriodicalIF":2.6,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000503996","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37444986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}