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Synthesis and Complexation of Monotosylated 4-Aminopyridine with Nickel (II) and Iron (II) Ions 镍(II)和铁(II)离子单基化4-氨基吡啶的合成与络合
IF 0.5 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2021-09-30 DOI: 10.7454/mss.v25i3.1242
kingsley John Orie, R. Duru, R. Ngochindo
Tosylated 4-aminopyridine and other sulfonylated compounds of amines comprise a substantial class of pharmaceutical drugs used as antibiotics in the field of medicine. This research aimed at the synthesis of tosylated 4-aminopyridine and the complexation of the tosyated 4-aminopyridine with Ni(II) and Fe(II) ions. The sulfonamide was prepared by the action of tosyl chloride on 4-aminopyridine in an aqueous alkaline medium. The complexes were synthesized by the reaction of Ni(NO3)2.6H2O /Fe(NO3)2.6H2O with sulfonamide derivative. These compounds were characterized through Ultraviolet Visible spectroscopy (UV–Vis), Fourier Transform Infer-Red (FTIR) spectroscopy, Proton Nuclear Magnetic Resonance (1HNMR), Carbon-13 Nuclear Magnetic Resonance (CNMR) and Electron Spray Ionisation-Mass Spectrometer (ESIMS) and micro-analysis. The IR spectral data suggested that the sulfonamide derivative acts as a neutral ligand towards Ni (II) and Fe (II). In their complexes, the coordination frequency bands of 1665.55 and 1674.21 cm− were assigned to Ni−N and Fe−N bonds, and 1687.70 cm− was assigned to free tosylated 4-aminopyridine. This decrease in the frequency band of free imine to coordinated imine complexes indicates that electron transfer occurred from the ligand to the d-orbitals of the metals. The complexation of4-Methyl-N-(pyridin-4-yl)benzene sulfonamide can increase the biological and catalytic potential of the ligand in the pharmaceutical and chemical industries.
甲酰基化4-氨基吡啶和其他磺化胺化合物构成了医学领域中用作抗生素的大量药物。本研究旨在合成甲酰基化4-氨基吡啶,并与Ni(II)和Fe(II)离子络合。在碱水介质中,氯甲酰与4-氨基吡啶反应制得磺胺。用Ni(NO3)2.6H2O /Fe(NO3)2.6H2O与磺胺衍生物反应合成配合物。通过紫外可见光谱(UV-Vis)、傅里叶变换红外光谱(FTIR)、质子核磁共振(1HNMR)、碳-13核磁共振(CNMR)、电子喷雾电离质谱仪(ESIMS)和微量分析对化合物进行了表征。红外光谱数据表明,磺胺衍生物作为Ni (II)和Fe (II)的中性配体,在它们的配合物中,1665.55和1674.21 cm−被分配给Ni−N和Fe−N键,1687.70 cm−被分配给游离甲基化4-氨基吡啶。自由亚胺到配位亚胺配合物的频带减小表明电子从配体转移到金属的d轨道。4-甲基- n-(吡啶-4-酰基)苯磺酰胺络合可以提高配体在制药和化学工业中的生物和催化潜力。
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引用次数: 3
Poly(2-Hydroxyethyl Methacrylate) Hydrogels for Contact Lens Applications–A Review 隐形眼镜用聚(2-羟乙基甲基丙烯酸酯)水凝胶综述
IF 0.5 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2021-09-30 DOI: 10.7454/mss.v25i3.1237
K. Saptaji, Nurlaely Rohmatul Iza, Sinta Widianingrum, Vania Katherine, Mulia, Iwan Setiawan
The emerging technology in biomedical engineering requires biocompatible materials, which are also referred to as biomaterials. For a material to be considered biocompatible, it should not interact with human tissues in a harmful way, and vice versa. Various properties of biocompatible materials, such as mechanical and optical properties, have to be considered for different biomedical applications. One of the most popular applications of biomaterials is for contact lenses. Hydrogels, specifically poly(2-hydroxyethyl methacrylate) (PHEMA) hydrogels, are among the most popular ones in ophthalmologic applications, especially in soft contact lenses. This paper reviews the use of PHEMA hydrogels as one of the important biomaterials. The possible applications, properties, and manufacturing process of PHEMA hydrogels, especially in contact lens applications, are addressed. Many studies have shown that PHEMA hydrogels possess many advantages in contact lens applications and have promising development prospects.
生物医学工程中的新兴技术需要生物相容性材料,也称为生物材料。对于被认为具有生物相容性的材料,它不应该以有害的方式与人体组织相互作用,反之亦然。生物相容性材料的各种特性,如机械和光学特性,必须考虑到不同的生物医学应用。生物材料最受欢迎的应用之一是隐形眼镜。水凝胶,特别是聚(2-羟乙基甲基丙烯酸酯)(PHEMA)水凝胶,是眼科应用中最受欢迎的一种,特别是在软性隐形眼镜中。本文综述了PHEMA水凝胶作为一种重要的生物材料的应用。讨论了PHEMA水凝胶的可能应用、性能和制造工艺,特别是在隐形眼镜应用中的应用。许多研究表明,PHEMA水凝胶在隐形眼镜应用中具有许多优势,具有广阔的发展前景。
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引用次数: 2
COVID-19 In Silico Drug with Zingiber officinale Natural Product Compound Library Targeting the Mpro Protein 以Mpro蛋白为靶点的姜黄天然产物化合物文库制备的新型COVID-19芯片药物
IF 0.5 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2021-09-30 DOI: 10.7454/mss.v25i3.1244
Renadya Maulani Wijaya, Muhammad Aldino Hafidzhah, V. D. Kharisma, A. Ansori, A. A. Parikesit
Coronavirus disease 2019 (COVID-19), which is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has become a worldwide pandemic. Ginger (Zingiber officinale) is a rhizome, which is commonly used for culinary and medicinal purposes. In Indonesia, ginger is taken as traditional medicine by processing it into a drink known as jamu. The present study aimed to assess and evaluate the bioactive compounds in ginger that can be used in drug design for treating COVID-19. The crystal structure of the SARS-CoV-2 main protease (Mpro) was generated from a protein sequence database, i.e., Protein Data Bank, and the bioactive compounds in ginger were derived from the existing compounds library. Mpro is involved in polyprotein synthesis, including viral maturation and nonstructural protein gluing, making it a potential antiviral target. Furthermore, the bioactive compounds in ginger were analyzed using Lipinski’s rule of five to determine their drug-like molecular properties. Moreover, molecular docking analysis was conducted using the Python Prescription 0.8 (Virtual Screening Tool) software, and the interaction between SARS-CoV-2 Mpro and the bioactive compounds in ginger was extensively examined using the PyMOL software. Out all of the 16 bioactive compounds that were docked successfully, 4-gingerol, which has the lowest binding energy against SARS-CoV-2 Mpro, as per the virtual screening results, was proven to have the most potential as a viral inhibitor of SARS-CoV-2. © 2021, Universitas Indonesia. All rights reserved.
由严重急性呼吸系统综合征冠状病毒2 (SARS-CoV-2)引起的冠状病毒病2019 (COVID-19)已成为全球大流行。姜(Zingiber officinale)是一种根茎,通常用于烹饪和药用目的。在印度尼西亚,生姜被加工成一种被称为jamu的饮料,被视为传统药物。本研究旨在评估和评价生姜中可用于治疗COVID-19药物设计的生物活性化合物。SARS-CoV-2主蛋白酶(Mpro)的晶体结构来源于蛋白质序列数据库protein Data Bank,生姜中的生物活性化合物来源于已有的化合物库。Mpro参与多蛋白合成,包括病毒成熟和非结构蛋白粘合,使其成为潜在的抗病毒靶点。此外,利用利平斯基的五法则对生姜中的生物活性成分进行了分析,以确定它们的类药物分子性质。利用Python Prescription 0.8 (Virtual Screening Tool)软件进行分子对接分析,并利用PyMOL软件广泛检测SARS-CoV-2 Mpro与生姜中生物活性化合物的相互作用。在所有对接成功的16种生物活性化合物中,根据虚拟筛选结果,对SARS-CoV-2 Mpro的结合能最低的4-姜辣素被证明是最有潜力作为SARS-CoV-2病毒抑制剂。©2021,印度尼西亚大学。版权所有。
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引用次数: 38
Analysis of Total Acidity toward Bacterial and Endophytic Fungi Profile dur-ing Black Garlic Processing from Garlic (Allium sativum L.) and Shallot (Allium ascalonicum L.) 大蒜和青葱黑蒜加工过程中总酸度对细菌和内生真菌的影响分析
IF 0.5 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2021-09-30 DOI: 10.7454/mss.v25i3.1220
A. Lestari, S. Wonorahardjo, S. Suharti
Black garlic or shallot are products of processed garlic and shallots obtained through a heating process conducted over a certain period. Black garlic/shallots have a mild aroma with a sweet and sour taste. The heating process causes chemical compound transition in the garlic, including acidity. In addition to the chemical process, the garlic’s color and component changes are due to the role of microorganisms during black garlic processing. However, the presence and function of such microorganisms have not been identified. Therefore, this research explores the black garlic’s microorganisms, their role in black garlic processing, and their relation to the total acidity changes. Total acidity test was completed using the potentiometric titration method, while the onion’s microorganisms were explored through isolation and characterization. Data show that black garlic’s total acidity of both garlic and shallot increases during the heating period day by day. Endophytic microbes that were successfully isolated during black garlic processing were observed on days 0 and 6. According to the rough data, the bacteria that emerged on day 0 are presumed to come from genus Erwinia, Pseudomonas, Xanthomonas, Agrobacterium, Ralstonia, Xylophilus, Pantoea, Acidovorax, Burkholderia, Coryneform, and Streptomyces, while the bacteria observed on day 6 are assumed to be generated from genus Coryneform and Streptomyces.
黑蒜或大葱是大蒜和大葱经过一定时间的加热加工而成的产品。黑蒜/青葱香气温和,酸甜适中。加热过程会导致大蒜中的化合物转变,包括酸度。除了化学过程,大蒜的颜色和成分的变化是由于微生物在黑蒜加工过程中的作用。然而,这些微生物的存在和功能尚未被确定。因此,本研究探讨了黑蒜中的微生物及其在黑蒜加工中的作用,以及它们与总酸度变化的关系。用电位滴定法测定洋葱的总酸度,并对洋葱的微生物进行分离和鉴定。数据显示,黑蒜在加热期间,大蒜和大葱的总酸度均呈上升趋势。在黑蒜加工过程中成功分离的内生微生物在第0天和第6天进行了观察。根据粗略数据,推测第0天出现的细菌为Erwinia属、Pseudomonas属、Xanthomonas属、Agrobacterium属、Ralstonia属、Xylophilus属、Pantoea属、Acidovorax属、Burkholderia属、Coryneform属和Streptomyces属,第6天出现的细菌为Coryneform属和Streptomyces属。
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引用次数: 1
Analysis on the Crystal Structure of the Piezoelectric Nanocrystal Ceramic of Pr-doped Bi1/2Na1/2TiO3 using Molten-Salt Synthesis 熔融盐法合成掺pr Bi1/2Na1/2TiO3压电纳米陶瓷的晶体结构分析
IF 0.5 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2021-09-30 DOI: 10.7454/mss.v25i3.1246
S. Ahda, Vita Ambarwati, Mardiyanto, E. Sukirman, B. Sugeng
One of the future technologies in nuclear reactors is the ability of piezoelectric materials to monitor reactor cores as sensors. Particularly, Pb-free piezoelectric materials, such as Bi1/2Na1/2TiO3, have been examined to improve the ability of their piezoelectric properties. In this study, we attempted to add Pr6O11 dopant material with mole ratios of 0, 0.3, 0.5, and 0.7 mol%. The synthesis process used in this study is the molten-salt synthesis method with a NaCl and KCl salt mole ratio of 1:1. The crystal structure analysis using the refinement process of the Rietveld method of the HighScore software was performed. Accordingly, a rhombohedral crystal structure system with lattice parameters of 3.8809(2), 3.8831(2), 3.8834(7), and 3.8816(2) angstroms and variations in the content of Pr6O11 was obtained. The optimal lattice parameter was discovered with the addition of 0.5 mol% of Pr6O11. The morphology of the synthesis product is still unclear for each addition of dopant material due to the large number of granular agglomerations.
未来的核反应堆技术之一是压电材料作为传感器监测反应堆堆芯的能力。特别是对Bi1/2Na1/2TiO3等无铅压电材料进行了研究,以提高其压电性能。在本研究中,我们尝试添加摩尔比为0、0.3、0.5和0.7 mol%的Pr6O11掺杂材料。本研究采用的合成工艺为NaCl与KCl盐摩尔比为1:1的熔融盐合成法。采用HighScore软件的Rietveld法细化过程进行晶体结构分析。由此得到了晶格参数分别为3.8809(2)、3.8831(2)、3.8834(7)和3.8816(2)埃且随Pr6O11含量变化的菱形体晶体结构体系。当Pr6O11的加入量为0.5 mol%时,发现了最佳的晶格参数。由于大量的颗粒团块,每次添加掺杂材料合成产物的形貌仍然不清楚。
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引用次数: 0
Yeasts Isolated from Traditional Brem Bali Show Stress Tolerance Phenotype against Fermentation-Related Stresses 从传统巴利酵母中分离的酵母对发酵相关的胁迫表现出耐受性表型
IF 0.5 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2021-01-01 DOI: 10.7454/MSS.V25I1.1209
Audria Bayu Lenka, R. Astuti, S. Listiyowati
The search for microbes, primarily yeasts with unique characters such as the tolerance against fermentation-relatedstresses, is gaining significant interest nowadays. Traditionally made alcoholic beverages can be used as sources for such yeasts, given that during fermentation and storage, microbes may develop stress tolerance responses leading to naturally stress-tolerant yeast strains. In this study, we used an alcoholic beverage, that is, Brem Bali, as the source of potential yeast isolates. We isolated nine yeast isolates from two traditional Brem Bali products. All isolates showed tolerance against high glucose stress (40–50%) and sensitivity against high-temperature stress (37–50 °C). Notably, isolate BT5 showed tolerance phenotype against ethanol stress (up to 12.5%). Notably, the ethanol stress tolerance phenotype shown by isolate BT5 was unlikely correlated to the ability of the isolates in combating other stresses. Based on the internal transcribed spacer sequence, isolates BT2, BT5, and BT6 shared high similarity with Wickerhamomyces anomalus (98%). Further approaches may be needed to clarify the identity of these isolates. Based on our study, isolate BT5 bears potential as a fermentation agent based on its ability to combat high glucose and ethanol stresses.
寻找微生物,主要是具有独特特性的酵母,如对发酵相关压力的耐受性,目前正引起人们极大的兴趣。传统制造的酒精饮料可以作为这种酵母的来源,因为在发酵和储存过程中,微生物可能会产生耐受性反应,从而产生自然的耐受性酵母菌株。在这项研究中,我们使用一种酒精饮料,即Brem Bali,作为潜在酵母菌分离物的来源。从两种传统的Brem Bali产品中分离出9株酵母菌。所有分离株均表现出高糖耐受性(40-50%)和高温敏感性(37-50℃)。值得注意的是,分离物BT5对乙醇胁迫表现出耐受性表型(高达12.5%)。值得注意的是,分离物BT5显示的乙醇耐受性表型不太可能与分离物抵抗其他胁迫的能力相关。根据内部转录的间隔序列,分离物BT2、BT5和BT6与Wickerhamomyces anomalus具有高度的相似性(98%)。可能需要进一步的方法来澄清这些分离株的身份。根据我们的研究,分离BT5具有作为发酵剂的潜力,基于其对抗高葡萄糖和乙醇应激的能力。
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引用次数: 2
Modeling of Aqueous Root Extract Compounds of Ruellia tuberosa L. for Alpha-Glucosidase Inhibition Through in Silico Study 通过计算机模拟模拟秋莲根水提物对α -葡萄糖苷酶的抑制作用
IF 0.5 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2021-01-01 DOI: 10.7454/MSS.V25I1.1223
A. Safitri, Dewi Ratih Tirto Sari, F. Fatchiyah, A. Roosdiana
This study aims to analyze the inhibitory activities of aqueous root extract compounds of Ruellia tuberosa L. toward alphaglucosidase protein by computational docking analysis. Three major compounds contained in the extracts (i.e., betaine, daidzein, and hispidulin) were selected as ligands; quercetin and acarbose were used as the reference. Computational docking analysis was performed using the HEX 8.0.0 program and visualized using the Discovery Studio Visualizer v19.1.0.18287 (2019 version) on the basis of the scoring functions. The interactions between ligands and alpha-glucosidase protein showed different binding patterns. The types of bonds involved in the interaction between the enzyme and these ligands were hydrogen and hydrophobic bonds. Energy generated from docking of betaine, daidzein, hispidulin, quercetin, and acarbose to alpha-glucosidase protein were −167.6, −249.5, −251.2, −241.5, and −322.1 cal/mol, respectively. Acarbose had the lowest energy, indicating that it has the strongest interaction with alpha-glucosidase, followed by hispidulin, daidzein, quercetin, and betaine. Amino acid residues that interacted with the ligands were His717, Met363, Arg608, Pro361, Phe362, Leu865, Glu869, Arg594, andAsp356. The current research shows that R. tuberosa L. aqueous root extracts have the potential to be used as an inhibitor for the alpha-glucosidase protein and as an antidiabetic agent. Nonetheless, further studies are needed to support this modeling study.
本研究旨在通过计算对接分析,分析秋莲根水提物对α -葡萄糖苷酶蛋白的抑制活性。选择甜菜碱、大豆苷元和hispidulin三种主要化合物作为配体;以槲皮素和阿卡波糖为对照物。基于评分函数,使用HEX 8.0.0程序进行计算对接分析,并使用Discovery Studio Visualizer v19.1.0.18287(2019版)进行可视化。配体与α -葡萄糖苷酶蛋白的相互作用表现出不同的结合模式。酶与这些配体相互作用的键的类型是氢键和疏水键。甜菜碱、大豆苷元、hispidulin、槲皮素和阿卡波糖与α -葡萄糖苷酶蛋白对接产生的能量分别为- 167.6、- 249.5、- 251.2、- 241.5和- 322.1 cal/mol。阿卡波糖的能量最低,说明它与α -葡萄糖苷酶的相互作用最强,其次是hispidulin、大豆黄酮、槲皮素和甜菜碱。与配体相互作用的氨基酸残基有His717、Met363、Arg608、Pro361、Phe362、Leu865、Glu869、Arg594和asp356。目前的研究表明,结核根水提取物具有作为α -葡萄糖苷酶蛋白抑制剂和抗糖尿病药物的潜力。尽管如此,还需要进一步的研究来支持这一模型研究。
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引用次数: 2
Synthesis of 1-(4-Ethoxy-3-methoxybenzyl)-1,10-phenanthrolin-1-ium Bromide and Its Evaluation as Antiplasmodium through Heme Polymerization Inhibitory Activity (HPIA) Assay 1-(4-乙氧基-3-甲氧基苄基)-1,10-菲罗啉-1-溴化ium的合成及其抗疟原虫血红素聚合抑制活性(HPIA)测定
IF 0.5 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2021-01-01 DOI: 10.7454/MSS.V25I1.1017
D. Fitriastuti, Viny Alfiyah, M. Mustofa, J. Jumina, M. Mardjan
This study describes the development of N-benzyl-1,10-phenantrolinium salt as an antiplasmodium agent. The salt, that is, 1-(4-ethoxy-3-methoxybenzyl)-1,10-phenanthrolin-1-ium bromide, was prepared using vanillin as the starting material in four simple synthetic steps. First, the alkylation of vanillin using diethyl sulfate produced 4-ethoxy-3methoxybenzaldehyde in 79% yield. Second, the reduction of the protected vanillin by NaBH4 through the grinding method allowed us to obtain 4-ethoxy-3-methoxybenzyl alcohol in 96% yield. Next, the bromination of the benzyl alcohol under solvent-free condition led to the formation of the corresponding benzyl bromide, which in turn underwent bimolecular nucleophilic substitution with 1,10-phenanthroline to produce the desired product, that is, 1-(4-ethoxy-3methoxybenzyl)-1,10-phenanthrolin-1-ium bromide, in 58% yield. The evaluation of N-benzyl-1,10-phenantrolinium salt as an antiplasmodium agent was conducted through heme polymerization inhibitory activity (HPIA) assay. The results showed that the phenantroline salt and chloroquine displayed the HPIA half maximal inhibitory concentrations of 3.63 and 4.37 mM, respectively. Therefore, 1-(4-ethoxy-3-methoxybenzyl)-1,10-phenanthrolin-1-ium bromide displays desirable HPIA and has a great potential to be further developed as an antiplasmodium.
本研究描述了n -苄基-1,10-非硝唑盐作为抗疟原虫剂的研制。以香兰素为原料,经过四个简单的合成步骤,制备了盐,即1-(4-乙氧基-3-甲氧基苄基)-1,10-菲罗啉-1-溴化ium。首先,用硫酸二乙酯烷基化香兰素,以79%的收率制得4-乙氧基-3甲氧基苯甲醛。其次,用NaBH4研磨法还原保护香兰素,得到收率为96%的4-乙氧基-3-甲氧基苄基醇。接下来,在无溶剂条件下,苯甲醇溴化生成相应的溴苄,溴苄又与1,10-菲罗啉进行双分子亲核取代,得到所需的产物,即1-(4-乙氧基-3甲氧基苄基)-1,10-菲罗啉-1-溴化ium,收率为58%。采用血红素聚合抑制活性(hbia)法对n -苄基-1,10-苯硝唑盐的抗疟原虫作用进行了评价。结果表明,菲安特林盐和氯喹的HPIA半最大抑制浓度分别为3.63和4.37 mM。因此,1 -(4-乙氧基-3-甲氧基苄基)-1,10-菲罗啉-1-溴化ium具有良好的抗疟原虫活性,具有进一步开发的潜力。
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引用次数: 0
Effect of Chromium Metal Accumulation on the Magnesium Absorption and Chlorophyll Content in Vegetables 金属铬积累对蔬菜镁吸收和叶绿素含量的影响
IF 0.5 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2021-01-01 DOI: 10.7454/MSS.V25I1.1176
Widya Sartika Sulistiani, Heningtyas Widowati, K. Sari, A. Sutanto
This study analyzed the effect of chromium metal accumulation on magnesium absorption and chlorophyll content in vegetables. The effect of accumulation was determined by performing controlled experimental methods on planting media supplemented with chromium and by directly observing vegetables grown in chromium-polluted areas, such as mountain, rice field, street, and industrial areas. The controlled experiments were carried out by varying the chromium contamination (1 and 3 ppm) and magnesium nutrition (0.4, 0.6, and 0.8 g/L) in planting media. The controlled experiment was compared with the results of field observation in several chromium-polluted areas. The effect of the treatment was analyzed based on the chlorophyll and magnesium levels in the leaves in comparison with the chromium levels in the planting medium. The results of observation and controlled experiments showed that the accumulation of chromium in plants affected the absorption of magnesium, which also affected chlorophyll formation and thus disrupted plant growth. The high chromium level (3 ppm) and magnesium level in planting media can reduce the accumulation of chromium in kale stems and leaves by 19% and 33%, respectively, increase magnesium absorption on kale stems and leaves by 7% and 5%, respectively, and increase chlorophyll formation on kale stems and leaves by 12% and 11%, respectively. Field observation in several chromium-polluted areas showed that spinach has a better chromium accumulation tolerance than kale in terms of magnesium absorption. The type of planting media, plant species, and the presence of other metal contaminants also affect chromium accumulation, magnesium absorption, and chlorophyll level.
本研究分析了金属铬积累对蔬菜镁吸收和叶绿素含量的影响。通过对照试验方法,在添加铬的种植介质上进行试验,并直接观察在铬污染地区(如山区、稻田、街道和工业区)种植的蔬菜,确定其积累效应。对照试验通过改变种植介质中铬污染(1和3 ppm)和镁营养(0.4、0.6和0.8 g/L)进行。将对照试验与若干铬污染地区的野外观测结果进行了比较。通过叶片中叶绿素和镁含量与种植介质中铬含量的比较,分析了处理的效果。观察和对照实验结果表明,铬在植物体内的积累影响了镁的吸收,镁的吸收也影响了叶绿素的形成,从而破坏了植物的生长。高铬(3 ppm)和高镁处理能使羽衣甘蓝茎叶中铬的积累分别减少19%和33%,使羽衣甘蓝茎叶中镁的吸收分别增加7%和5%,使羽衣甘蓝茎叶中叶绿素的形成分别增加12%和11%。在几个铬污染地区的田间观察表明,菠菜在镁吸收方面比羽衣甘蓝具有更好的耐铬积累能力。种植介质类型、植物种类和其他金属污染物的存在也会影响铬的积累、镁的吸收和叶绿素水平。
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引用次数: 0
Cloning of pe11 (LipX, Rv1169c) gene of Mycobacterium tuberculosis Beijing strain to pcDNA3.1 plasmid vector 结核分枝杆菌北京株pe11 (LipX, Rv1169c)基因在pcDNA3.1质粒载体上的克隆
IF 0.5 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2021-01-01 DOI: 10.7454/MSS.V25I1.1206
Lulut Azmi Supardi, Andriansjah Rukmana, Fithriyah Sjatha
Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis. It is a persistent global health problem with a high mortality rate. Currently, TB is controlled by administering the Bacillus Calmette-Guerin (BCG) vaccine, but the effectiveness of its protection varies among individuals in a population. The pe/ppe gene family comprises a typical group of genes that play a role in avoiding the host immune response and inducing persistent TB infection. Based on in silico analysis, the pe11 gene has estimated immunogenicity and potential as a TB seed vaccine candidate. The pe11 gene from an Indonesian isolate of an M. tuberculosis Beijing strain was amplified by polymerase chain reaction (PCR) and inserted into the mammalian expression vector pcDNA3.1. The recombinant vector pcDNA3.1-pe11 was used to transform Top10 competent Escherichia coli. Clones from the transformation were subjected to colony PCR to confirm the direction of the insert. Sequencing was performed to confirm the correctness of the insert sequence. In this study, the pe11 gene was successfully cloned into the pcDNA3.1 vector in the correct direction to assure PE11 expression. No mutations were found in the pe11 gene insert, compared with the M. tuberculosis H37Rv sequence as the standard. A pcDNA3.1 vector containing the pe11 gene derived from an M. tuberculosis Beijing strain was successfully constructed.
结核病(TB)是一种由结核分枝杆菌引起的传染病。这是一个长期存在的全球健康问题,死亡率很高。目前,结核病是通过接种卡介苗来控制的,但其保护效果因人群中的个体而异。pe/ppe基因家族包括一组典型的基因,在避免宿主免疫反应和诱导持续结核感染方面发挥作用。基于硅片分析,pe11基因估计具有免疫原性和作为结核种子疫苗候选物的潜力。采用聚合酶链反应(PCR)扩增印尼结核分枝杆菌北京株pe11基因,并将其插入哺乳动物表达载体pcDNA3.1中。利用重组载体pcDNA3.1-pe11转化Top10大肠杆菌。对转化后的克隆进行菌落PCR以确定插入物的方向。测序以确认插入序列的正确性。本研究成功将pe11基因沿正确方向克隆到pcDNA3.1载体中,保证了pe11的表达。与结核分枝杆菌H37Rv序列比较,pe11基因插入片段未发现突变。成功构建了结核分枝杆菌北京株pe11基因pcDNA3.1载体。
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引用次数: 0
期刊
Makara Journal of Science
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