Pub Date : 2014-11-01DOI: 10.1109/MHS.2014.7006168
Jiafeng Yao, Tatsuya Kodera, A. Sapkota, H. Obara, M. Takei
Impedance spectroscopy has been proved to be a useful technique for analyzing heterogeneous systems, especially biological cell suspensions and tissues because of its capacity of non-invasive measurement. In the present paper, experiments were conducted to study the dielectric properties of yeast cells by Impedance Spectroscopy. The capacitance of the suspension of yeast cells was measured and the relative permittivity and the dielectric loss were calculated. As the concentration of yeast cells increased, the relative permittivity and the dielectric loss were also increased. In addition, the relative permittivity and the dielectric loss of living cells were higher than that of the dead ones. Finally, we concluded that it is possible to sense the status and the concentration of cell suspension by impedance spectroscopy.
{"title":"Experimental study on dielectric properties of yeast cells in micro channel by impedance spectroscopy","authors":"Jiafeng Yao, Tatsuya Kodera, A. Sapkota, H. Obara, M. Takei","doi":"10.1109/MHS.2014.7006168","DOIUrl":"https://doi.org/10.1109/MHS.2014.7006168","url":null,"abstract":"Impedance spectroscopy has been proved to be a useful technique for analyzing heterogeneous systems, especially biological cell suspensions and tissues because of its capacity of non-invasive measurement. In the present paper, experiments were conducted to study the dielectric properties of yeast cells by Impedance Spectroscopy. The capacitance of the suspension of yeast cells was measured and the relative permittivity and the dielectric loss were calculated. As the concentration of yeast cells increased, the relative permittivity and the dielectric loss were also increased. In addition, the relative permittivity and the dielectric loss of living cells were higher than that of the dead ones. Finally, we concluded that it is possible to sense the status and the concentration of cell suspension by impedance spectroscopy.","PeriodicalId":181514,"journal":{"name":"2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125493982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-11-01DOI: 10.1109/MHS.2014.7006137
Masanobu Kito, H. Maruyama, F. Arai
We developed measurement method of oxygen consumption rate (OCR) of individual oocyte using stripe-shape fluorescence sensor on a microfluidic chip. The fluorescence sensor was made of the mixture of polyethylene glycol diacrylate (PEG-DA) and Tris (2,2'-bipyridyl) dichloro ruthenium (II) hexahydrate (Ru(bpy)3Cl2). The sensor was photo-patterned to stripe-shape in the polydimethylsiloxane (PDMS). Oxygen around the oocyte can be spherically diffused because of high oxygen permeability of PDMS and the stripe pattern of sensor. This sensor achieves two-dimensional measurement of OCR by image processing. We demonstrated non-contact measurement of OCR of single oocyte on a microfluidic chip.
{"title":"Non-contact measurement of oxygen consumption rate of single oocyte using stripe-shaped fluorescence sensor on microfluidic chip","authors":"Masanobu Kito, H. Maruyama, F. Arai","doi":"10.1109/MHS.2014.7006137","DOIUrl":"https://doi.org/10.1109/MHS.2014.7006137","url":null,"abstract":"We developed measurement method of oxygen consumption rate (OCR) of individual oocyte using stripe-shape fluorescence sensor on a microfluidic chip. The fluorescence sensor was made of the mixture of polyethylene glycol diacrylate (PEG-DA) and Tris (2,2'-bipyridyl) dichloro ruthenium (II) hexahydrate (Ru(bpy)3Cl2). The sensor was photo-patterned to stripe-shape in the polydimethylsiloxane (PDMS). Oxygen around the oocyte can be spherically diffused because of high oxygen permeability of PDMS and the stripe pattern of sensor. This sensor achieves two-dimensional measurement of OCR by image processing. We demonstrated non-contact measurement of OCR of single oocyte on a microfluidic chip.","PeriodicalId":181514,"journal":{"name":"2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122560834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-11-01DOI: 10.1109/MHS.2014.7006082
Masakazu Honda, H. Karakawa, Koichi Akahori, T. Miyaoka, M. Ohka
In order to utilize the phenomenon of kinesthetic illusion as a human interface capable of presenting kinesthetic sense, it is necessary to define specification of the vibrator required to elicit kinesthetic illusion. In this study, the stimulation thresholds of the illusion have been investigated using the staircase method, which is an adaptive psychometric method. The result of the experiment reveals that the kinesthetic illusion can be elicited with an acceleration of about 40 [m/s2], with vibratory stimuli of 50 to 90 [Hz]. Furthermore, at the highest frequency of 120 [Hz] the illusion cannot be elicited unless the acceleration is increased to 60 [m/s2].
{"title":"Estimation of vibration stimulus threshold for inducing kinesthetic illusion","authors":"Masakazu Honda, H. Karakawa, Koichi Akahori, T. Miyaoka, M. Ohka","doi":"10.1109/MHS.2014.7006082","DOIUrl":"https://doi.org/10.1109/MHS.2014.7006082","url":null,"abstract":"In order to utilize the phenomenon of kinesthetic illusion as a human interface capable of presenting kinesthetic sense, it is necessary to define specification of the vibrator required to elicit kinesthetic illusion. In this study, the stimulation thresholds of the illusion have been investigated using the staircase method, which is an adaptive psychometric method. The result of the experiment reveals that the kinesthetic illusion can be elicited with an acceleration of about 40 [m/s2], with vibratory stimuli of 50 to 90 [Hz]. Furthermore, at the highest frequency of 120 [Hz] the illusion cannot be elicited unless the acceleration is increased to 60 [m/s2].","PeriodicalId":181514,"journal":{"name":"2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":"87 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124946404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-11-01DOI: 10.1109/MHS.2014.7006089
S. Sugiura, F. Yanagawa, T. Takagi, K. Sumaru, T. Kanamori, M. Tamura, H. Matsui
In this work, we present a novel photocleavable crosslinker to form photodegradable hydrogel through one step reaction by mixing with biocompatible polymers containing amino moieties (amino-terminated tetra-arm poly(ethylene glycol) or gelatin). Our new photocleavable crosslinker can be utilized as potentially versatile and convenient material to prepare photodegradable hydrogels with amino terminated biocompatible polymers. We present a couple of cell manipulation technique using the photodegradable hydrogels prepared with our photocleavable crosslinker.
{"title":"Microscale cell manipulation by using photodegradable hydrogel","authors":"S. Sugiura, F. Yanagawa, T. Takagi, K. Sumaru, T. Kanamori, M. Tamura, H. Matsui","doi":"10.1109/MHS.2014.7006089","DOIUrl":"https://doi.org/10.1109/MHS.2014.7006089","url":null,"abstract":"In this work, we present a novel photocleavable crosslinker to form photodegradable hydrogel through one step reaction by mixing with biocompatible polymers containing amino moieties (amino-terminated tetra-arm poly(ethylene glycol) or gelatin). Our new photocleavable crosslinker can be utilized as potentially versatile and convenient material to prepare photodegradable hydrogels with amino terminated biocompatible polymers. We present a couple of cell manipulation technique using the photodegradable hydrogels prepared with our photocleavable crosslinker.","PeriodicalId":181514,"journal":{"name":"2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133270458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-11-01DOI: 10.1109/MHS.2014.7006086
Yutaka Ikeda, Tomoki Yoshinari, Y. Nagasaki
Reactive oxygen species (ROS) play important roles in regulating cellular morphology. The balance between generation and elimination of ROS is essential for the maintenance of signaling pathways. Disturbances in the redox balance results in changes in cellular morphology and disturbs cellular homeostasis. Therefore, it is essential to suppress the excess ROS in order to maintain cellular homeostasis. In this work, we constructed new biocompatible surfaces based on ROS scavenging character and demonstrate the utility of a ROS-scavenging polymer as a novel biocompatible material.
{"title":"Impact of ROS scavenging effect on cell culture and bio assembler","authors":"Yutaka Ikeda, Tomoki Yoshinari, Y. Nagasaki","doi":"10.1109/MHS.2014.7006086","DOIUrl":"https://doi.org/10.1109/MHS.2014.7006086","url":null,"abstract":"Reactive oxygen species (ROS) play important roles in regulating cellular morphology. The balance between generation and elimination of ROS is essential for the maintenance of signaling pathways. Disturbances in the redox balance results in changes in cellular morphology and disturbs cellular homeostasis. Therefore, it is essential to suppress the excess ROS in order to maintain cellular homeostasis. In this work, we constructed new biocompatible surfaces based on ROS scavenging character and demonstrate the utility of a ROS-scavenging polymer as a novel biocompatible material.","PeriodicalId":181514,"journal":{"name":"2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133436060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-11-01DOI: 10.1109/MHS.2014.7006140
Hiroki Umeshima, Yuu Kure, Kenichi Nomura, S. Yoshikawa, S. Sakuma, F. Arai, M. Kaneko, M. Kengaku
In this paper, we present a novel method to investigate the mechanism driving nuclear translocation in migrating neurons via digital image analyses of the nucleus. We obtained and quantitatively analyzed time-lapse images of the fluorescently labeled nucleus with a high-speed confocal microscope. We focused on spatiotemporal dynamics of the boundary curvature of the nucleus during nuclear translocation. Our data suggest major contribution of a pulling force to nuclear translocation during neuronal migration.
{"title":"Quantitative image analyses of nuclear dynamics in migrating neurons","authors":"Hiroki Umeshima, Yuu Kure, Kenichi Nomura, S. Yoshikawa, S. Sakuma, F. Arai, M. Kaneko, M. Kengaku","doi":"10.1109/MHS.2014.7006140","DOIUrl":"https://doi.org/10.1109/MHS.2014.7006140","url":null,"abstract":"In this paper, we present a novel method to investigate the mechanism driving nuclear translocation in migrating neurons via digital image analyses of the nucleus. We obtained and quantitatively analyzed time-lapse images of the fluorescently labeled nucleus with a high-speed confocal microscope. We focused on spatiotemporal dynamics of the boundary curvature of the nucleus during nuclear translocation. Our data suggest major contribution of a pulling force to nuclear translocation during neuronal migration.","PeriodicalId":181514,"journal":{"name":"2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132111669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-11-01DOI: 10.1109/MHS.2014.7006151
H. Ohgane, T. Takebe, K. Sekine, H. Taniguchi
A huge number of publications reported the production of immature hepatocyte-like cells by sequential addition of multiple inductive factors; however, little comparative analyses were performed. The purpose of this study is to identify the optimal induction system using human induced pluripotent stem cells (iPSCs). Initially, based on the published outcomes, functional screen was performed to select the three promising step-wise differentiation protocols. Then, cellular morphology, hepatocyte marker gene expressions, and secreted protein production of cells derived from the each specific protocol were characterized at final maturation stage. Through these efforts, we identified a highly efficient protocol for deriving hepatocyte-like cells. In combination with our recently developed 3-D culture systems (organ bud generation method), mature hepatocytes will be derived from human iPSCs with markedly increased efficiency in an artificially reconstituted human liver by way of liver bud transplantation.
{"title":"Efficient generation of mature hepatocyte-like cells from human iPSCs","authors":"H. Ohgane, T. Takebe, K. Sekine, H. Taniguchi","doi":"10.1109/MHS.2014.7006151","DOIUrl":"https://doi.org/10.1109/MHS.2014.7006151","url":null,"abstract":"A huge number of publications reported the production of immature hepatocyte-like cells by sequential addition of multiple inductive factors; however, little comparative analyses were performed. The purpose of this study is to identify the optimal induction system using human induced pluripotent stem cells (iPSCs). Initially, based on the published outcomes, functional screen was performed to select the three promising step-wise differentiation protocols. Then, cellular morphology, hepatocyte marker gene expressions, and secreted protein production of cells derived from the each specific protocol were characterized at final maturation stage. Through these efforts, we identified a highly efficient protocol for deriving hepatocyte-like cells. In combination with our recently developed 3-D culture systems (organ bud generation method), mature hepatocytes will be derived from human iPSCs with markedly increased efficiency in an artificially reconstituted human liver by way of liver bud transplantation.","PeriodicalId":181514,"journal":{"name":"2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132146099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-11-01DOI: 10.1109/MHS.2014.7006115
S. C. Abdullah, M. Ohka, J. Mahmud, M. A. M. Jusoh, J. Saedon
This paper presents an image processing capable of detecting basic shapes that communicate as intelligent agent by active vision sensor system. We also propose image recognition algorithm to facilitate a binocular camera to be able to detect and recognize multi geometrical object shapes. We investigate within plain and crowded background environment, indoor and outdoor along with low noise and very high noise that complicate the edge shape. The evaluation result shows plain environment give the highest recognition percentage followed by indoor and outdoor. Meanwhile the unsatisfied result is due to the high noises that make the gradient magnitude cannot distinguish between the multi geometrical edges and background because of their similar or shared pixels. However, the image processing efficiency is improved and able to distinguish image in real time navigation and amenable to spatial agent memory architectures. In near future, the result shall be used as a capstone for future application and development in humanoid robot sensor field.
{"title":"Multi geometrical image processing based on active vision agent","authors":"S. C. Abdullah, M. Ohka, J. Mahmud, M. A. M. Jusoh, J. Saedon","doi":"10.1109/MHS.2014.7006115","DOIUrl":"https://doi.org/10.1109/MHS.2014.7006115","url":null,"abstract":"This paper presents an image processing capable of detecting basic shapes that communicate as intelligent agent by active vision sensor system. We also propose image recognition algorithm to facilitate a binocular camera to be able to detect and recognize multi geometrical object shapes. We investigate within plain and crowded background environment, indoor and outdoor along with low noise and very high noise that complicate the edge shape. The evaluation result shows plain environment give the highest recognition percentage followed by indoor and outdoor. Meanwhile the unsatisfied result is due to the high noises that make the gradient magnitude cannot distinguish between the multi geometrical edges and background because of their similar or shared pixels. However, the image processing efficiency is improved and able to distinguish image in real time navigation and amenable to spatial agent memory architectures. In near future, the result shall be used as a capstone for future application and development in humanoid robot sensor field.","PeriodicalId":181514,"journal":{"name":"2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":"248 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115010279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-11-01DOI: 10.1109/MHS.2014.7006104
N. Tanaka, M. Kondo, H. Sugiyama, M. Yamato, T. Okano, Jun Miyake
This study assessed the wettability of cellular surface with noncontact meniscus manipulation under a microscope. The surface of culture medium covered on cells was manipulated by air-jet and the meniscus of culture medium was generated. The movement of meniscus was monitored with a microscope. The shape of interface between culture medium and air on the cells was different from the part of cells.
{"title":"Surface-tension microscopy by noncontact meniscus-manipulation","authors":"N. Tanaka, M. Kondo, H. Sugiyama, M. Yamato, T. Okano, Jun Miyake","doi":"10.1109/MHS.2014.7006104","DOIUrl":"https://doi.org/10.1109/MHS.2014.7006104","url":null,"abstract":"This study assessed the wettability of cellular surface with noncontact meniscus manipulation under a microscope. The surface of culture medium covered on cells was manipulated by air-jet and the meniscus of culture medium was generated. The movement of meniscus was monitored with a microscope. The shape of interface between culture medium and air on the cells was different from the part of cells.","PeriodicalId":181514,"journal":{"name":"2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130283818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-11-01DOI: 10.1109/MHS.2014.7006136
T. Hayakawa, S. Sakuma, F. Arai
We present a cell manipulation method by using vibration induced whirling flow. The local flow is induced just around a micropillar by applying circular vibration to the micropillar. By patterning a micropillar array on a chip and applying circular vibration to the chip, flow for cell transport can be generated along the array. As an application, we demonstrate a single cell extraction using the vibration-induced flow and single cell catcher made by thermo-responsive gel. Target cells are transported to the single cell catcher by vibration induced flow and caught by the single cell catcher. We tried 30 times single cell extraction, succeeded in 100% single cell catch and 60% extraction to external culture well.
{"title":"On-chip cell manipulation by vibration-induced whirling flow","authors":"T. Hayakawa, S. Sakuma, F. Arai","doi":"10.1109/MHS.2014.7006136","DOIUrl":"https://doi.org/10.1109/MHS.2014.7006136","url":null,"abstract":"We present a cell manipulation method by using vibration induced whirling flow. The local flow is induced just around a micropillar by applying circular vibration to the micropillar. By patterning a micropillar array on a chip and applying circular vibration to the chip, flow for cell transport can be generated along the array. As an application, we demonstrate a single cell extraction using the vibration-induced flow and single cell catcher made by thermo-responsive gel. Target cells are transported to the single cell catcher by vibration induced flow and caught by the single cell catcher. We tried 30 times single cell extraction, succeeded in 100% single cell catch and 60% extraction to external culture well.","PeriodicalId":181514,"journal":{"name":"2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130334893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：