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2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)最新文献

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Non-contact 3D rotation and capture method for bio-object based on microfluidic stream 基于微流控的生物物体非接触式三维旋转与捕获方法
Pub Date : 2014-11-01 DOI: 10.1109/MHS.2014.7006149
Yaxiaer Yalikun, T. Asano, Y. Kanda, K. Morishima
This paper reports a simple method to capture and rotate a bio-object at a controllable speed in direction of vertical plane. This method intends to implement its function without contact in an open space for biological application such as dynamically monitoring, orientation setting, and mechanical stimulation induced by fluidic shear force. To verify this principle, calculation process of necessary flow rate for generating enough operating force on the cell is conducted with CFD. An open-space microchip with several orifices (diameter is 100 μm) is designed and fabricated. Then the manipulating demonstrations are conducted. On the calculated conditions, the rotation of target cell is successfully obtained. The relation between the object's velocity, flow rate, and operating force is clarified.
本文报道了一种捕获生物物体并使其沿垂直方向以可控速度旋转的简单方法。该方法旨在实现其在开放空间中的无接触功能,如动态监测、定向设置和流体剪切力诱导的机械刺激。为了验证这一原理,利用CFD进行了对电池产生足够操作力所需流量的计算过程。设计并制作了一种具有多个孔径(直径为100 μm)的开放式微芯片。然后进行了操纵演示。在计算条件下,成功地获得了目标单元的旋转。阐明了物体的速度、流量和作用力之间的关系。
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引用次数: 1
Construction of cell sheet-based 3D tissues with designed cell orientation using anisotropic cell sheets 利用各向异性细胞片构建具有设计细胞取向的基于细胞片的三维组织
Pub Date : 2014-11-01 DOI: 10.1109/MHS.2014.7006060
Hironobu Takahashi, Tatsuya Shimizu, Masamichi Nakayama, M. Yamato, T. Okano
In this study, the cell sheet-based technology was able to control cell orientation in 3D engineered tissue construct. Using a micropatterned thermoresponsive surface, human cells such as fibroblasts and myoblasts were aligned on the surface, and manipulated as a single cell sheet by reducing the culture temperature to 20°C. Consequently, their anisotropic cell sheets can be layered using gelatin gel to produce 3D tissue constructs with the desired anisotropy. For example, two fibroblast sheets layered perpendicularly showed three-dimensionally different cell orientation as designed. By induce differentiation of myoblasts forming an anisotropic cell sheet into myotubes, a myotube construct with a single orientation was possible to be created. Since the combined use of the anisotropic cell sheet and cell sheet manipulation technique allows us to create complex tissue that requires the three-dimensional control of their anisotropies, we believe that it has a potential to be one of the next-generation tissue engineering technology.
在这项研究中,基于细胞片的技术能够控制三维工程组织结构中的细胞取向。使用微图案热响应表面,将成纤维细胞和成肌细胞等人类细胞排列在表面,并通过降低培养温度至20℃作为单个细胞片进行操作。因此,它们的各向异性细胞片可以使用明胶凝胶分层,以产生具有所需各向异性的3D组织结构。例如,垂直分层的两个成纤维细胞片显示出设计的三维不同的细胞取向。通过诱导形成各向异性细胞片的成肌细胞分化为肌管,可以形成具有单一取向的肌管结构。由于各向异性细胞片和细胞片操作技术的结合使用使我们能够创建需要对其各向异性进行三维控制的复杂组织,我们相信它有潜力成为下一代组织工程技术之一。
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引用次数: 0
Levitation energy of piezoelectric actuator using a levitation mechanism 利用悬浮机构的压电驱动器的悬浮能
Pub Date : 2014-11-01 DOI: 10.1109/MHS.2014.7006110
S. Sone, A. Torii, K. Doki, S. Mototani
In this research, we focused on the energy consumed in the levitation mechanism used in an inchworm-type microactuator when the mechanism is levitated. We use a power meter for the measurement of the energy required for levitation. We estimate the levitation height by comparing the levitation height measured by a displacement sensor and the power consumption measured by a power meter. The levitation height measured with the displacement sensor shows the similar trend obtained with the power used in the levitation mechanism. We are studying a piezoelectric actuator used in a levitation mechanism. The levitation mechanism levitates using a squeeze effect caused by the vertical vibration of the piezoelectric element. Frictional force control is realized by using levitation mechanisms, and the microactuator moves by the principle of inchworm. We usually measure the displacement of the levitation mechanisms by the use of a displacement sensor. However, the measurement of the vertical direction is difficult because there are a number of limitations. Therefore, a measurement method without displacement sensors in the vertical direction is required. The estimation method proposed in this paper can extend the working range of the microactuators. The energy consumed in the microactuator includes the information of the actuator. We measure the energy and the state simultaneously, and levitation height used for levitation is estimated. The measured and estimated levitation height as a function of the operation frequency is also discussed.
在本研究中,我们重点研究了一种微型执行器所使用的悬浮机构在悬浮状态下所消耗的能量。我们用功率计来测量悬浮所需的能量。我们通过比较位移传感器测量的悬浮高度和功率计测量的功耗来估计悬浮高度。位移传感器测得的悬浮高度与悬浮机构所用功率的变化趋势相似。我们正在研究一种用于悬浮机构的压电致动器。悬浮机构利用压电元件垂直振动产生的挤压效应悬浮。采用悬浮机构实现摩擦力控制,微执行器采用尺蠖运动原理。我们通常用位移传感器来测量悬浮机构的位移。然而,垂直方向的测量是困难的,因为有许多限制。因此,需要一种没有垂直方向位移传感器的测量方法。本文提出的估计方法可以扩大微执行器的工作范围。所述微致动器中消耗的能量包括所述致动器的信息。我们同时测量了能量和状态,并估计了用于悬浮的悬浮高度。并讨论了测量和估计的悬浮高度随运行频率的变化规律。
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引用次数: 0
Cell pairing on a microwell array electrode by positive dielectrophoresis 微孔阵列电极上的细胞配对
Pub Date : 2014-11-01 DOI: 10.1109/MHS.2014.7006159
T. Yasukawa, Yukinori Yoshimura, F. Mizutani
We present single-cell pairing of different types of cells with a rapid manipulation based on positive dielectrophoresis (p-DEP). The DEP device for the manipulation of cells consisted of a microfluidic channel with an upper indium tin oxide (ITO) electrode and a lower ITO electrode with 10,000 (100 × 100) microwells. The width (14 μm) and depth (25 μm) of the individual microwells restricted the size to two vertically aligned cells. Cells stained in blue and stained in green were continuously trapped in the microwells. Cells were paired within only 1 min and a pairing efficiency of 53% was achieved.
我们提出单细胞配对不同类型的细胞与快速操作的基础上,正介电电泳(p-DEP)。用于细胞操作的DEP装置由一个带有上氧化铟锡(ITO)电极的微流控通道和一个带有10,000 (100 × 100)微孔的下ITO电极组成。单个微孔的宽度(14 μm)和深度(25 μm)限制了两个垂直排列的细胞的尺寸。用蓝色和绿色染色的细胞被连续地捕获在微孔中。细胞在1分钟内配对,配对效率达到53%。
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引用次数: 0
Evaluation of cell-cell or cell-substrate adhesion effect on cellular differentiation using a microwell array having convertible culture surface 利用具有可转换培养表面的微孔阵列评估细胞-细胞或细胞-底物对细胞分化的粘附效应
Pub Date : 2014-11-01 DOI: 10.1109/MHS.2014.7006132
Y. Nakashima, Kohichi Tsusu, Yuki Hikichi, Tairo Yokokura, K. Minami, Y. Nakanishi
In this paper, effects of cell-cell adhesion or cell-substrate adhesion on cellular differentiation are evaluated using a microwell array having convertible culture surface (CCS microwell array). The CCS microwell array is used for limitation of culture area and regulation of the adhesion timing. The CCS microwell array is fabricated by photolithography of SU-8 photoresist on the cover slip, and coating of the alginate thin film on the microwell bottoms. The alginate thin film is used as a material that inhibits adhesion of cells. It uses an alginate film that is not toxic to cells and can be removed by ethylendiaminetetraacetate (EDTA), which is calcium chelator, at arbitrary timing during cell culture. A single spheroid is formed by cell-cell adhesion in microwells because cells can't adhere to the culture surface by adhesion inhibition effect of the alginate thin film. When the culture surface is converted to cell-adhesiveness surface by removing alginate thin film, cells adhere to the microwell bottoms. The transition of the diameter of spheroid cultured in the CCS microwell array was evaluated by observation of an inverted microscope. Also, cellular differentiation behavior under four kinds of conditions was compared for verification of cell-cell or cell-substrate adhesion effect. This result shows the efficiency of induction of cellular differentiation was increased by cell culture in the CCS microwell array compared with three kinds of other culture conditions.
本文利用具有可转换培养表面的微孔阵列(CCS微孔阵列)评估了细胞-细胞粘附或细胞-底物粘附对细胞分化的影响。CCS微孔阵列用于限制培养面积和调节粘附时间。采用SU-8光刻胶在盖板上光刻,并在微孔底部涂覆海藻酸盐薄膜的方法制备了CCS微孔阵列。海藻酸盐薄膜被用作抑制细胞粘附的材料。它使用一种对细胞无毒的海藻酸盐膜,可以在细胞培养过程中任意时间被钙螯合剂乙二胺四乙酸酯(EDTA)去除。藻酸盐薄膜的抑制粘附作用使细胞不能粘附在培养物表面,微孔中细胞-细胞粘附形成单一的球体。通过去除海藻酸盐薄膜,将培养表面转化为细胞粘附表面,细胞粘附在微孔底部。通过倒置显微镜观察CCS微孔阵列培养的球体直径的转变。同时,比较了四种条件下的细胞分化行为,验证了细胞-细胞或细胞-基质的粘附效应。结果表明,与其他三种培养条件相比,CCS微孔阵列培养的细胞诱导分化效率更高。
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引用次数: 0
Generation of rotational flow in microfluidics device for formation of spheroid 微流体球体形成装置中旋转流的产生
Pub Date : 2014-11-01 DOI: 10.1109/MHS.2014.7006134
M. Kojima, M. Horade, H. Takai, K. Ohara, T. Tanikawa, K. Kamiyama, Y. Mae, T. Arai
Spheroid is formed by making it agitate and circle in cell suspension. However, the injection of the cells used as pieces of spheroids, the recovery at the time of tissue formation, exchange of a culture medium, etc. are performed manually, and it is hard to say that efficient organization construction is performed. Thus, we suggest utility and possibility of applying microfluidics for formation of toroidal-like spheroid. Microfluidic devices to generate rotational flow were fabricated, and we conducted experiments using mouse embryo fibroblast cell.
球体是在细胞悬浮液中通过搅拌和旋转形成的。然而,作为球体块的细胞注射、组织形成时的恢复、培养基的交换等都是手工进行的,很难说进行了高效的组织构建。因此,我们建议应用微流体技术来形成类环面球体的实用性和可能性。制备了微流控装置,并利用小鼠胚胎成纤维细胞进行了实验。
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引用次数: 0
Leakage-free reconfigurable microchannel having moving sidewalls sealed with hydrocarbon sealant and oil seals 无泄漏可重构微通道,其移动侧壁采用碳氢密封胶和油封密封
Pub Date : 2014-11-01 DOI: 10.1109/MHS.2014.7006079
Masahiro Oono, Kousuke Mikami, N. Futai
In conventional micro-fluidic devices, it is difficult to transport gaseous and solid as well as liquid, or change the size of the channel depending on cell proliferation. We have developed a reconfigurable microfluidic channel with movable sidewalls by mechanically discretized sidewalls with laterally aligned rectangular pins. However, the leakage of fluid through the gap between pins by capillary action is problematic. To address these problems, we have introduced hydrocarbon wax and silicone adhesive to fill the gap, and have studied the effect of pin-to-pin gaps and the surface roughness of pins on the leakage.
在传统的微流体装置中,很难传输气体和固体以及液体,或者根据细胞增殖改变通道的大小。我们开发了一种具有可移动侧壁的可重构微流体通道,通过机械离散的侧壁与横向排列的矩形销。然而,由于毛细作用,流体通过销间间隙泄漏是有问题的。为了解决这些问题,我们引入了碳氢蜡和硅酮胶粘剂来填补间隙,并研究了引脚间隙和引脚表面粗糙度对泄漏的影响。
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引用次数: 0
Design of CuO anti-reflection structure for thin-film thermoelectric generator 薄膜热电发电机CuO增透结构设计
Pub Date : 2014-11-01 DOI: 10.1109/MHS.2014.7006118
T. Kondou, M. Mizoshiri, S. Hata
Copper oxide anti-reflection structures were designed by rigorous coupled wave analysis for thin-film thermoelectric solar generator. We proposed thin-film thermoelectric generator with the anti-reflection structure on the hot side of pn junction. When infrared solar light from 800 to 1200 nm wavelength is irradiated to the thermoelectric generator, the anti-reflection structure on the hot side generates the temperature gradient between the hot side and cold side of pn junction. When the cross-sectional geometry of the anti-reflection structures was hemisphere which was geometrically approximated pyramid one, the reflectance from 800 to 1200 nm wavelength was reduced than flat and binary ones. The reflectance of solar light was obtained by considering the solar light spectra and its polarization. By assuming that the average of TM and TE polarized light was the reflectance of solar light, the reflectance was reduced to be 9.6% when the period of the anti-reflection structure was 700 nm. Taking into account of the reflectance of Cu on cold side 99%, this large reflectance difference is expected to generate a large temperature gradient between hot side and cold side.
采用严格耦合波分析方法,设计了薄膜热电太阳能发电机的氧化铜增透结构。提出了在pn结热侧具有增透结构的薄膜热电发生器。当800 ~ 1200nm波长的太阳红外光照射到热电发生器上时,热侧的增透结构在pn结热侧和冷侧之间产生温度梯度。当增透结构的横截面几何形状为半球形时,其在800 ~ 1200nm波段的反射率比平面和二元结构的反射率降低。通过考虑太阳光谱及其偏振,得到了太阳光的反射率。假设TM和TE偏振光的平均值为太阳光的反射率,则增透结构周期为700 nm时,反射率降为9.6%。考虑到Cu在冷侧的反射率为99%,这种较大的反射率差预计会在热侧和冷侧之间产生较大的温度梯度。
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引用次数: 0
Measurement of cellular reactive force on a microfluidic chip using moiré fringe 微流控芯片上细胞反作用力的测量
Pub Date : 2014-11-01 DOI: 10.1109/MHS.2014.7006154
Hirotaka Sugiura, S. Sakuma, M. Kaneko, F. Arai
In this paper we propose the method for the measurement of cellular mechanical response on a microfluidic chip. In order to improve the sensing resolution, we applied the principle of moiré fringe. As a result, the resolution of the measurement is approximately ten times higher than our previous research.
本文提出了一种在微流控芯片上测量细胞力学响应的方法。为了提高传感分辨率,我们采用了莫尔条纹原理。因此,测量的分辨率大约是我们以前研究的十倍。
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引用次数: 0
Development of face recognition algorithm for enhancement of social communication of robotic assistive autism therapy 机器人辅助自闭症治疗中增强社交交流的人脸识别算法的开发
Pub Date : 2014-11-01 DOI: 10.1109/MHS.2014.7006062
Mohd Ridzuan Abdul Samat, S. Shamsuddin, M. Miskam, H. Yussof
In this paper, we propose a face recognition method for tracking, detecting and recognizing the faces of children with Autism Spectrum Disorder (ASD) for robotic assistive therapy application. ASD is a brain developmental disorder that manifests itself through a person's behavior and social-communication skill [1]. Face recognition stage is an essential element in robotic assistive therapy since it is the first interaction step that occurs between autistic children and robot. The algorithm aims to encourage autistic children to interact and to capture their attention using a robot that recognizes their faces. This is to establish a beneficial training environment for autistic children before engaging further in the training module.
在本文中,我们提出了一种人脸识别方法,用于跟踪、检测和识别自闭症谱系障碍(ASD)儿童的面部,用于机器人辅助治疗。ASD是一种通过个体行为和社交技能表现出来的大脑发育障碍[1]。人脸识别阶段是自闭症儿童与机器人互动的第一步,是机器人辅助治疗的重要组成部分。该算法旨在鼓励自闭症儿童与他人互动,并通过一个能识别他们面部的机器人来吸引他们的注意力。这是为了在进一步参与培训模块之前,为自闭症儿童建立一个有益的培训环境。
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引用次数: 3
期刊
2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)
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