Pub Date : 2014-11-01DOI: 10.1109/MHS.2014.7006066
Daichi Matsuoka, G. Enriquez, Huei Ee Yap, S. Hashimoto
Hyper-redundant manipulators are an interesting solution for flexible manipulations. While these types of manipulators can be used in complex environments, often their weight becomes impractically heavy due to the large number of actuators required for a large number of degrees of freedom. Biomimetic manipulators have received attention as a possible solution for the above problem, by imitating biological systems in which a large number of joints are driven by cooperation of muscles. However, these approaches often don't achieve the desired weight reduction as a whole system, due to the need for an air compressor. As such, the purpose of our research is a hyper-redundant manipulator designed with the intention of weight saving over the whole system.
{"title":"Development of a lightweight manipulator with constraint mechanism","authors":"Daichi Matsuoka, G. Enriquez, Huei Ee Yap, S. Hashimoto","doi":"10.1109/MHS.2014.7006066","DOIUrl":"https://doi.org/10.1109/MHS.2014.7006066","url":null,"abstract":"Hyper-redundant manipulators are an interesting solution for flexible manipulations. While these types of manipulators can be used in complex environments, often their weight becomes impractically heavy due to the large number of actuators required for a large number of degrees of freedom. Biomimetic manipulators have received attention as a possible solution for the above problem, by imitating biological systems in which a large number of joints are driven by cooperation of muscles. However, these approaches often don't achieve the desired weight reduction as a whole system, due to the need for an air compressor. As such, the purpose of our research is a hyper-redundant manipulator designed with the intention of weight saving over the whole system.","PeriodicalId":181514,"journal":{"name":"2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132329278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-11-01DOI: 10.1109/MHS.2014.7006161
M. A. M. Jusoh, M. Ohka, Yu Chun Wang, T. Miyaoka
Due to the increasing number of rehabilitation product related to human-robotic interfaces, tactile sensation will provide excellent hints on the research progress in robotic field. The objective of this research is to study on the decisive factor of human being in differentiating the thickness between two thin foils; which is the stainless steel and copper. In this study, the finite element (FE) simulation of the fingertip's 3D elastic model has been performed by the aid of CATIA V5. From calculated results, we examined the displacement rate of the foil. As a result, the displacement rate for copper (which was monitored at the foil surface) is always higher compared to stainless steel. By comparing with previous psychophysics result, the simulation also proves similar traits; where human subjects discriminate thickness of foil according to material hardness when t <; 100 μm, thickness is undetermined when 100 <; t <; 350 μm and evaluation is done via another system when t > 350 μm.
{"title":"Understanding of human tactile mechanism in comparing material properties through the analysis of displacement on foil surface","authors":"M. A. M. Jusoh, M. Ohka, Yu Chun Wang, T. Miyaoka","doi":"10.1109/MHS.2014.7006161","DOIUrl":"https://doi.org/10.1109/MHS.2014.7006161","url":null,"abstract":"Due to the increasing number of rehabilitation product related to human-robotic interfaces, tactile sensation will provide excellent hints on the research progress in robotic field. The objective of this research is to study on the decisive factor of human being in differentiating the thickness between two thin foils; which is the stainless steel and copper. In this study, the finite element (FE) simulation of the fingertip's 3D elastic model has been performed by the aid of CATIA V5. From calculated results, we examined the displacement rate of the foil. As a result, the displacement rate for copper (which was monitored at the foil surface) is always higher compared to stainless steel. By comparing with previous psychophysics result, the simulation also proves similar traits; where human subjects discriminate thickness of foil according to material hardness when t <; 100 μm, thickness is undetermined when 100 <; t <; 350 μm and evaluation is done via another system when t > 350 μm.","PeriodicalId":181514,"journal":{"name":"2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132359334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-11-01DOI: 10.1109/MHS.2014.7006067
Kosei Endo, T. Anada, M. Yamada, M. Seki, K. Sasaki, O. Suzuki
This study was designed to investigate whether octacalcium phosphate (OCP) in alginate (Alg) composite promotes osteoblastic differentiation of mouse bone marrow stromal ST-2 cells and composite releases the cells outside after incubation. The results suggested that Alg/OCP composite could be a good device for bone tissue engineering.
{"title":"In vitro assessment of osteoblastic differentiation of encapsulated stromal cells in alginate/octacalcium phosphate","authors":"Kosei Endo, T. Anada, M. Yamada, M. Seki, K. Sasaki, O. Suzuki","doi":"10.1109/MHS.2014.7006067","DOIUrl":"https://doi.org/10.1109/MHS.2014.7006067","url":null,"abstract":"This study was designed to investigate whether octacalcium phosphate (OCP) in alginate (Alg) composite promotes osteoblastic differentiation of mouse bone marrow stromal ST-2 cells and composite releases the cells outside after incubation. The results suggested that Alg/OCP composite could be a good device for bone tissue engineering.","PeriodicalId":181514,"journal":{"name":"2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129441538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-11-01DOI: 10.1109/MHS.2014.7006098
Akira Fukushima, M. Kojima, K. Ohara, M. Horade, K. Kamiyama, Y. Mae, T. Arai
Recently, several studies have reported, the in vitro fabrication of small scale 3D tissues. However, in large cell structures, oxygen and nutrients are not supplied to the inside of the tissue. In this case, cells at the center will die. We propose an automated system for the construction of a fine lattice shaped structure, based on hydrogel fiber to produce active 3D tissues in vitro. To evaluate the proposed system, a fine lattice structure was constructed as a basic experiment of 3D tissue form.
{"title":"Automated construction system for fine lattice shape structures based on alginate gel fiber","authors":"Akira Fukushima, M. Kojima, K. Ohara, M. Horade, K. Kamiyama, Y. Mae, T. Arai","doi":"10.1109/MHS.2014.7006098","DOIUrl":"https://doi.org/10.1109/MHS.2014.7006098","url":null,"abstract":"Recently, several studies have reported, the in vitro fabrication of small scale 3D tissues. However, in large cell structures, oxygen and nutrients are not supplied to the inside of the tissue. In this case, cells at the center will die. We propose an automated system for the construction of a fine lattice shaped structure, based on hydrogel fiber to produce active 3D tissues in vitro. To evaluate the proposed system, a fine lattice structure was constructed as a basic experiment of 3D tissue form.","PeriodicalId":181514,"journal":{"name":"2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":" 15","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133021760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-11-01DOI: 10.1109/MHS.2014.7006139
Kazuya Tashiro, T. Masuda, F. Arai
In order to achieve detection of flowing cells in the glass capillary, we proposed the integrated micropipette. In this research, we focused on the fabrication of microcapillary. The fabrication method is based on MEMS packaging technique. Two glass layer; a micro channel and a sensor layer are bonded, and we can get microcapillary with capacitance sensor for detection of cell flowing. By using fabricated microcapillary, we successfully achieved aspirating and detecting of cells flowing in a microchannel.
{"title":"Single-cell picking by integrated micropipette having sensor for passage detection of cell","authors":"Kazuya Tashiro, T. Masuda, F. Arai","doi":"10.1109/MHS.2014.7006139","DOIUrl":"https://doi.org/10.1109/MHS.2014.7006139","url":null,"abstract":"In order to achieve detection of flowing cells in the glass capillary, we proposed the integrated micropipette. In this research, we focused on the fabrication of microcapillary. The fabrication method is based on MEMS packaging technique. Two glass layer; a micro channel and a sensor layer are bonded, and we can get microcapillary with capacitance sensor for detection of cell flowing. By using fabricated microcapillary, we successfully achieved aspirating and detecting of cells flowing in a microchannel.","PeriodicalId":181514,"journal":{"name":"2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132391331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-11-01DOI: 10.1109/MHS.2014.7006160
M. Imai, Takeomi Mizutani, K. Kawabata, H. Haga
Epithelial cells are known to form structures, such as cysts and tubes, with three-dimensional (3-D) morphologies. During formation of the morphologies of these structures, apico-basal polarity is regulated by secretion of laminins and integrins. In this study, we observed epithelial cells with mushroom-like 3-D morphologies; many of these cells had tail structures, where endogenous laminin-α3, laminin-β1, and integrin-β1 were found to be accumulated. Interestingly, time-lapse observation of the cells showed rotational movement around the tail. We also observed the process of change from the single-cell morphology to the mushroom-like morphology. Additionally, we observed that single cells showed an elongated protrusion as a predecessor of the tail structure. In addition to laminin-α3, laminin-β1, and integrin-β1, laminin-β3 was also found to be localized in the protrusion. These results indicate that the apico-basal polarization and regulation of laminin secretion are crucial for the formation of the mushroom-like structure and for the rotational movement of Madin-Darby canine kidney (MDCK) cells cultured on Matrigel.
{"title":"Secretion of different altered laminin isoforms results in three-dimensional morphological changes in cells cultured on Matrigel","authors":"M. Imai, Takeomi Mizutani, K. Kawabata, H. Haga","doi":"10.1109/MHS.2014.7006160","DOIUrl":"https://doi.org/10.1109/MHS.2014.7006160","url":null,"abstract":"Epithelial cells are known to form structures, such as cysts and tubes, with three-dimensional (3-D) morphologies. During formation of the morphologies of these structures, apico-basal polarity is regulated by secretion of laminins and integrins. In this study, we observed epithelial cells with mushroom-like 3-D morphologies; many of these cells had tail structures, where endogenous laminin-α3, laminin-β1, and integrin-β1 were found to be accumulated. Interestingly, time-lapse observation of the cells showed rotational movement around the tail. We also observed the process of change from the single-cell morphology to the mushroom-like morphology. Additionally, we observed that single cells showed an elongated protrusion as a predecessor of the tail structure. In addition to laminin-α3, laminin-β1, and integrin-β1, laminin-β3 was also found to be localized in the protrusion. These results indicate that the apico-basal polarization and regulation of laminin secretion are crucial for the formation of the mushroom-like structure and for the rotational movement of Madin-Darby canine kidney (MDCK) cells cultured on Matrigel.","PeriodicalId":181514,"journal":{"name":"2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125101576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-11-01DOI: 10.1109/MHS.2014.7006144
Song Won Eui, T. Masuda, H. Nakanishi, F. Arai
We succeeded in isolating pre-counted small amounts of circulating tumor cells from whole blood. And we also confirmed isolation of CTCs from mouse-CTC model with same method. On our open microfluidic chip, CTCs are isolated by their size-difference from other blood cells. Therefore, we arranged micropillars on the microfluidic chip. Blood sample is injected between chip and inclined coverglass. And then, by suction of blood sample, CTCs are drifted and collected. Near 90% of CTCs were isolated without any pretreatments. We also operated animal testing with same method.
{"title":"Open microfluidic chip using air-liquid interface for single cell isolation and aspiration","authors":"Song Won Eui, T. Masuda, H. Nakanishi, F. Arai","doi":"10.1109/MHS.2014.7006144","DOIUrl":"https://doi.org/10.1109/MHS.2014.7006144","url":null,"abstract":"We succeeded in isolating pre-counted small amounts of circulating tumor cells from whole blood. And we also confirmed isolation of CTCs from mouse-CTC model with same method. On our open microfluidic chip, CTCs are isolated by their size-difference from other blood cells. Therefore, we arranged micropillars on the microfluidic chip. Blood sample is injected between chip and inclined coverglass. And then, by suction of blood sample, CTCs are drifted and collected. Near 90% of CTCs were isolated without any pretreatments. We also operated animal testing with same method.","PeriodicalId":181514,"journal":{"name":"2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121816484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-11-01DOI: 10.1109/MHS.2014.7006069
M. Nakajima, Masaru Takeuchi, T. Masuda, T. Anada, O. Suzuki, T. Fukuda, Qiang Huang, Y. Hasegawa
Computer topography (CT) imaging is one of the powerful-tools for nondestructive observation technique. In this paper, we present observation of bone from mice by scanning electron microscope (SEM)-CT system using environmental- SEM (E-SEM). The advantage of this SEM-CT system is able to image in high-resolution with less than 400 nm resolution. We investigated the effects of X-ray wavelengths and intensities to improve the imaging contrast for SEM-CT observation. From experimental results, a porous structures were observed inside the bone sample with approximately 10 μm pores.
{"title":"SEM-CT observation of bone from mice using environmental-SEM","authors":"M. Nakajima, Masaru Takeuchi, T. Masuda, T. Anada, O. Suzuki, T. Fukuda, Qiang Huang, Y. Hasegawa","doi":"10.1109/MHS.2014.7006069","DOIUrl":"https://doi.org/10.1109/MHS.2014.7006069","url":null,"abstract":"Computer topography (CT) imaging is one of the powerful-tools for nondestructive observation technique. In this paper, we present observation of bone from mice by scanning electron microscope (SEM)-CT system using environmental- SEM (E-SEM). The advantage of this SEM-CT system is able to image in high-resolution with less than 400 nm resolution. We investigated the effects of X-ray wavelengths and intensities to improve the imaging contrast for SEM-CT observation. From experimental results, a porous structures were observed inside the bone sample with approximately 10 μm pores.","PeriodicalId":181514,"journal":{"name":"2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127089016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-11-01DOI: 10.1109/MHS.2014.7006143
Tetsuya Kaneko, R. Gomi, E. Sato-Shimokawara, Toru Yamaguchi
User's life-logs include various and useful information. Life-logs can be used for health care and improve daily living recasting his/her daily life. Our aim is monitoring a user's life for acquiring user's life-logs. In previous research, life-logs is acquired using user's GPS data or estimating daily activities. These methods are useful for estimation of what he/she did or where he/she went, however it is difficult to estimate user's likes and tastes. Therefore, this paper focuses on the dialogue with the robot. In this research, the authors experiment to what feature can be acquired through dialogue with the robot for the acquisition of the user's likes and tastes.
{"title":"Acquisition of the human characteristics through dialogue with a robot at home","authors":"Tetsuya Kaneko, R. Gomi, E. Sato-Shimokawara, Toru Yamaguchi","doi":"10.1109/MHS.2014.7006143","DOIUrl":"https://doi.org/10.1109/MHS.2014.7006143","url":null,"abstract":"User's life-logs include various and useful information. Life-logs can be used for health care and improve daily living recasting his/her daily life. Our aim is monitoring a user's life for acquiring user's life-logs. In previous research, life-logs is acquired using user's GPS data or estimating daily activities. These methods are useful for estimation of what he/she did or where he/she went, however it is difficult to estimate user's likes and tastes. Therefore, this paper focuses on the dialogue with the robot. In this research, the authors experiment to what feature can be acquired through dialogue with the robot for the acquisition of the user's likes and tastes.","PeriodicalId":181514,"journal":{"name":"2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":"125 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127324259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-11-01DOI: 10.1109/MHS.2014.7006103
Takaaki Mori, Yukihiro Akiyama, Shogo Unuma, T. Saito
We found a unique cell membrane perforation method which is applying photochemical oxidation on a limited area of the cell membrane, with high survival rate. We recently developed an nano needle array cell membrane perforator which is containing photosensitizer for mass cell processing and a robotic processing system. However, There were problems that the perforating processing efficiency for floating cells was low and that the sequential processing could not be performed in the method. In order to apply the perforating method to a cell therapy, we should devise the system components which can perform sequential perforation processing. In this report, we developed core components of the process for cell suspensions. The components are membrane filter and its supporting block, to support the cells during the perforation process and to pass through the medium. We evaluated the system with a fluorochrome introduction to the cells. With the system, we could inject membrane impermeable fluorescent dye AlexaFluor594 to the floating rat PC12 cells. This reveals that our sequential cell processing concept is adequate. By combining some flow control system for feeding the cell suspension, the system will be developed as an automatic bed-side cell processing system for various cell therapies.
{"title":"High performance cell membrane perforation system for cell suspensions","authors":"Takaaki Mori, Yukihiro Akiyama, Shogo Unuma, T. Saito","doi":"10.1109/MHS.2014.7006103","DOIUrl":"https://doi.org/10.1109/MHS.2014.7006103","url":null,"abstract":"We found a unique cell membrane perforation method which is applying photochemical oxidation on a limited area of the cell membrane, with high survival rate. We recently developed an nano needle array cell membrane perforator which is containing photosensitizer for mass cell processing and a robotic processing system. However, There were problems that the perforating processing efficiency for floating cells was low and that the sequential processing could not be performed in the method. In order to apply the perforating method to a cell therapy, we should devise the system components which can perform sequential perforation processing. In this report, we developed core components of the process for cell suspensions. The components are membrane filter and its supporting block, to support the cells during the perforation process and to pass through the medium. We evaluated the system with a fluorochrome introduction to the cells. With the system, we could inject membrane impermeable fluorescent dye AlexaFluor594 to the floating rat PC12 cells. This reveals that our sequential cell processing concept is adequate. By combining some flow control system for feeding the cell suspension, the system will be developed as an automatic bed-side cell processing system for various cell therapies.","PeriodicalId":181514,"journal":{"name":"2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":"66 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127028515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：