首页 > 最新文献

Materials science & engineering. C, Materials for biological applications最新文献

英文 中文
NIR and glutathione trigger the surface release of methotrexate linked by Diels-Alder adducts to anisotropic gold nanoparticles 近红外光谱和谷胱甘肽触发由Diels-Alder加合物连接到各向异性金纳米颗粒上的甲氨蝶呤的表面释放
IF 7.9 1区 工程技术 Q1 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2021-12-01 DOI: 10.1016/j.msec.2021.112512
Karen Bolaños , Macarena Sánchez-Navarro , Ernest Giralt , Gerardo Acosta , Fernando Albericio , Marcelo J. Kogan , Eyleen Araya

The administration and controlled release of drugs over time remains one of the greatest challenges of science today. In the nanomaterials field, anisotropic gold nanoparticles (AuNPs) with plasmon bands centered at the near-infrared region (NIR), such as gold nanorods (AuNRs) and gold nanoprisms (AuNPrs), under laser irradiation, locally increase the temperature, allowing the release of drugs. In this sense, temporally controlled drug delivery could be promoted by external stimuli using thermo-reversible chemical reactions, such as Diels-Alder cycloadditions from a diene and a dienophile fragment (compound a). In this study, an antitumor drug (methotrexate, MTX) was linked to plasmonic AuNPs by a Diels-Alder adduct (compound c), which after NIR suffers a retro-Diels-Alder reaction, producing release of the drug (compound b). We obtained two nanosystems based on AuNRs and AuNPrs. Both nanoconstructs were coated with BSA-r8 (Bovine Serum Albumin functionalized with Arg8, all-D octa arginine) in order to increase the colloidal stability and promote internalization of the nanosystems on HeLa and SK-BR-3 cells. In addition, the presence of BSA allows protecting the cargo from being released on the extracellular environment and promotes the photothermal release of the drug in the presence of glutathione (GSH). The nanosystems' drug release profile was evaluated after NIR irradiation in the presence and absence of glutathione (GSH), showing a considerable increase of drug release when NIR light and glutathione were combined. This work broadens the range of possibilities of using two complementary strategies for the controlled release of an antitumor drug from AuNRs and AuNPrs: the photothermal cleavage of a thermolabile adduct controlled by an external stimulus (laser irradiation), complemented with the use of the intracellular metabolite GSH.

随着时间的推移,药物的管理和控制释放仍然是当今科学面临的最大挑战之一。在纳米材料领域,具有等离子体带的各向异性金纳米粒子(AuNPs),如金纳米棒(AuNRs)和金纳米棱镜(aunpr),在激光照射下局部升高温度,从而使药物释放。从这个意义上说,暂时受控的药物递送可以通过热可逆化学反应的外部刺激来促进,例如Diels-Alder环从二烯和亲二烯片段(化合物a)中添加(化合物a)。在本研究中,一种抗肿瘤药物(甲氨蝶呤,MTX)通过Diels-Alder加合物(化合物c)与等离子体aunp连接,该化合物在近红外后发生反向Diels-Alder反应,产生药物释放(化合物b)。我们获得了基于aunr和aunpr的两种纳米系统。为了提高胶体稳定性,促进纳米系统在HeLa和SK-BR-3细胞上的内化,两种纳米结构都包被BSA-r8(用Arg8,全d八精氨酸功能化的牛血清白蛋白)。此外,牛血清白蛋白的存在可以保护货物不被释放到细胞外环境,并促进药物在谷胱甘肽(GSH)存在下的光热释放。在有谷胱甘肽(GSH)和没有谷胱甘肽(GSH)的情况下,对纳米系统进行近红外照射后的药物释放谱进行评估,结果表明,当近红外光和谷胱甘肽联合使用时,药物释放量显著增加。这项工作拓宽了使用两种互补策略从aunr和aunpr控制释放抗肿瘤药物的可能性范围:由外部刺激(激光照射)控制的热稳定性加合物的光热裂解,辅以细胞内代谢物GSH的使用。
{"title":"NIR and glutathione trigger the surface release of methotrexate linked by Diels-Alder adducts to anisotropic gold nanoparticles","authors":"Karen Bolaños ,&nbsp;Macarena Sánchez-Navarro ,&nbsp;Ernest Giralt ,&nbsp;Gerardo Acosta ,&nbsp;Fernando Albericio ,&nbsp;Marcelo J. Kogan ,&nbsp;Eyleen Araya","doi":"10.1016/j.msec.2021.112512","DOIUrl":"10.1016/j.msec.2021.112512","url":null,"abstract":"<div><p>The administration and controlled release of drugs over time remains one of the greatest challenges of science today. In the nanomaterials field, anisotropic gold nanoparticles (AuNPs) with plasmon bands centered at the near-infrared region (NIR), such as gold nanorods (AuNRs) and gold nanoprisms (AuNPrs), under laser irradiation, locally increase the temperature, allowing the release of drugs. In this sense, temporally controlled drug delivery could be promoted by external stimuli using thermo-reversible chemical reactions, such as Diels-Alder cycloadditions from a diene and a dienophile fragment (compound a). In this study, an antitumor drug (methotrexate, MTX) was linked to plasmonic AuNPs by a Diels-Alder adduct (compound c), which after NIR suffers a retro-Diels-Alder reaction, producing release of the drug (compound b). We obtained two nanosystems based on AuNRs and AuNPrs. Both nanoconstructs were coated with BSA-r<sub>8</sub> (Bovine Serum Albumin functionalized with Arg<sub>8</sub>, all-D octa arginine) in order to increase the colloidal stability and promote internalization of the nanosystems on HeLa and SK-BR-3 cells. In addition, the presence of BSA allows protecting the cargo from being released on the extracellular environment and promotes the photothermal release of the drug in the presence of glutathione (GSH). The nanosystems' drug release profile was evaluated after NIR irradiation in the presence and absence of glutathione (GSH), showing a considerable increase of drug release when NIR light and glutathione were combined. This work broadens the range of possibilities of using two complementary strategies for the controlled release of an antitumor drug from AuNRs and AuNPrs: the photothermal cleavage of a thermolabile adduct controlled by an external stimulus (laser irradiation), complemented with the use of the intracellular metabolite GSH.</p></div>","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":"131 ","pages":"Article 112512"},"PeriodicalIF":7.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0928493121006524/pdfft?md5=33efbb4efda86009f0c128738f628b43&pid=1-s2.0-S0928493121006524-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39686241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Cellular defense system-destroying nanoparticles as a platform for enhanced chemotherapy against drug-resistant cancer 破坏细胞防御系统的纳米颗粒作为增强化疗对抗耐药癌症的平台
IF 7.9 1区 工程技术 Q1 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2021-12-01 DOI: 10.1016/j.msec.2021.112494
Boyi Niu , Kaixin Liao , Yixian Zhou , Ting Wen , Guilan Quan , Chuanbin Wu , Xin Pan

Cellular defense system represented by glutathione (GSH) greatly weakens the outcomes of cancer therapy by antioxidation and detoxification. GSH depletion has been proved to be an effective way to enhance the efficacy of reactive oxygen species (ROS)-based therapies and chemotherapy. However, the existing strategies of GSH depletion still face the problems of unclear biosafety and high complexity of multicomponent co-delivery. In this study, we developed a GSH-depleting carrier platform based on disulfide-bridged mesoporous organosilica nanoparticles (MONs) to destroy the cellular defense system for cancer therapy. Responding to the high level of GSH in cancer cells, the disulfide bonds in the framework of MONs could be broken and consumed substantial GSH at the same time. Moreover, this process also promoted the degradation of MONs. In order to evaluate the effect of this platform in cancer therapy, chemotherapeutic drug cisplatin was loaded into MONs (Pt@MONs) to treat drug-resistant non-small cell lung cancer. In vitro and in vivo results indicated that Pt@MONs efficiently triggered GSH depletion, promoted platinum-DNA adduct formation, and induced cell apoptosis, resulting in significant tumor growth inhibition without marked toxicity. Taken together, the cellular defense system-destroying nanoparticles provide a promising platform for enhanced cancer therapy.

以谷胱甘肽(GSH)为代表的细胞防御系统通过抗氧化和解毒极大地削弱了癌症治疗的效果。谷胱甘肽耗竭已被证明是提高活性氧(ROS)治疗和化疗疗效的有效途径。然而,现有的谷胱甘肽消耗策略仍然面临生物安全性不明确和多组分共给药复杂性高的问题。在这项研究中,我们开发了一种基于二硫桥接介孔有机二氧化硅纳米颗粒(MONs)的gsh消耗载体平台,以破坏癌症治疗的细胞防御系统。对癌细胞中高水平的谷胱甘肽作出反应,MONs框架中的二硫键可以被破坏,同时消耗大量的谷胱甘肽。同时,这一过程也促进了蒙斯的降解。为了评估该平台在癌症治疗中的效果,将化疗药物顺铂装入MONs (Pt@MONs),用于耐药非小细胞肺癌的治疗。体外和体内实验结果表明,Pt@MONs有效触发GSH耗竭,促进铂- dna加合物形成,诱导细胞凋亡,显著抑制肿瘤生长,无明显毒性。总之,破坏细胞防御系统的纳米颗粒为增强癌症治疗提供了一个有希望的平台。
{"title":"Cellular defense system-destroying nanoparticles as a platform for enhanced chemotherapy against drug-resistant cancer","authors":"Boyi Niu ,&nbsp;Kaixin Liao ,&nbsp;Yixian Zhou ,&nbsp;Ting Wen ,&nbsp;Guilan Quan ,&nbsp;Chuanbin Wu ,&nbsp;Xin Pan","doi":"10.1016/j.msec.2021.112494","DOIUrl":"10.1016/j.msec.2021.112494","url":null,"abstract":"<div><p>Cellular defense system represented by glutathione (GSH) greatly weakens the outcomes of cancer therapy by antioxidation and detoxification. GSH depletion has been proved to be an effective way to enhance the efficacy of reactive oxygen species (ROS)-based therapies and chemotherapy. However, the existing strategies of GSH depletion still face the problems of unclear biosafety and high complexity of multicomponent co-delivery. In this study, we developed a GSH-depleting carrier platform based on disulfide-bridged mesoporous organosilica nanoparticles (MONs) to destroy the cellular defense system for cancer therapy. Responding to the high level of GSH in cancer cells, the disulfide bonds in the framework of MONs could be broken and consumed substantial GSH at the same time. Moreover, this process also promoted the degradation of MONs. In order to evaluate the effect of this platform in cancer therapy, chemotherapeutic drug cisplatin was loaded into MONs (Pt@MONs) to treat drug-resistant non-small cell lung cancer. <em>In vitro</em> and <em>in vivo</em> results indicated that Pt@MONs efficiently triggered GSH depletion, promoted platinum-DNA adduct formation, and induced cell apoptosis, resulting in significant tumor growth inhibition without marked toxicity. Taken together, the cellular defense system-destroying nanoparticles provide a promising platform for enhanced cancer therapy.</p></div>","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":"131 ","pages":"Article 112494"},"PeriodicalIF":7.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0928493121006342/pdfft?md5=bbb8b0785fddfbdfebaa34faead52b4e&pid=1-s2.0-S0928493121006342-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39775288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Plasma surface modification strategies for the preparation of antibacterial biomaterials: A review of the recent literature 制备抗菌生物材料的等离子体表面改性策略:近期文献综述
IF 7.9 1区 工程技术 Q1 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2021-12-01 DOI: 10.1016/j.msec.2021.112474
Ebru Akdoğan, Hasret Tolga Şirin

Plasma-based strategies offer several advantages for developing antibacterial biomaterials and can be used directly or combined with other surface modification techniques. Direct plasma strategies can be classified as plasma surface modifications that derive antibacterial property by tailoring surface topography or surface chemistry. Nano patterns induced by plasma modification can exhibit antibacterial property and promote the adhesion and proliferation of mammalian cells, creating antibacterial and biocompatible surfaces. Antibacterial effect by tailoring surface chemistry via plasma can be attained by either creating bacteriostatic surfaces or bactericidal surfaces. Plasma-assisted strategies incorporate plasma processes in combination with other surface modification techniques. Plasma coating can serve as a drug-eluting reservoir and diffusion barrier. The plasma-functionalized surface can serve as a platform for grafting antibacterial agents, and plasma surface activation can improve the adhesion of polymeric layers with antibacterial properties. This article critically reviews plasma-based strategies reported in the recent literature for the development of antibacterial biomaterial surfaces. Studies using both atmospheric and low-pressure plasmas are included in this review. The findings are discussed in terms of the trends in material and precursor selection, modification stability, antibacterial efficacy, the choice of bacterial strains tested, cell culture findings, critical aspects of in vitro performance testing and in vivo experimental design.

基于等离子体的策略为抗菌生物材料的开发提供了几个优势,可以直接使用或与其他表面改性技术相结合。直接等离子体策略可分为等离子体表面修饰,通过调整表面形貌或表面化学来获得抗菌性能。等离子体修饰诱导的纳米图案具有抗菌特性,可促进哺乳动物细胞的粘附和增殖,形成抗菌和生物相容性表面。通过等离子体裁剪表面化学的抗菌效果可以通过创建抑菌表面或杀菌表面来实现。等离子体辅助策略将等离子体过程与其他表面修饰技术相结合。等离子体涂层可以作为药物洗脱库和扩散屏障。等离子体功能化表面可以作为接枝抗菌剂的平台,等离子体表面活化可以提高具有抗菌性能的聚合物层的附着力。这篇文章批判性地回顾了在最近的文献中报道的基于等离子体的抗菌生物材料表面开发策略。本文综述了使用常压等离子体和低压等离子体的研究。研究结果讨论了材料和前体选择的趋势,修饰稳定性,抗菌功效,测试菌株的选择,细胞培养结果,体外性能测试和体内实验设计的关键方面。
{"title":"Plasma surface modification strategies for the preparation of antibacterial biomaterials: A review of the recent literature","authors":"Ebru Akdoğan,&nbsp;Hasret Tolga Şirin","doi":"10.1016/j.msec.2021.112474","DOIUrl":"10.1016/j.msec.2021.112474","url":null,"abstract":"<div><p>Plasma-based strategies offer several advantages for developing antibacterial biomaterials and can be used directly or combined with other surface modification techniques. Direct plasma strategies can be classified as plasma surface modifications that derive antibacterial property by tailoring surface topography or surface chemistry. Nano patterns induced by plasma modification can exhibit antibacterial property and promote the adhesion and proliferation of mammalian cells, creating antibacterial and biocompatible surfaces. Antibacterial effect by tailoring surface chemistry via plasma can be attained by either creating bacteriostatic surfaces or bactericidal surfaces. Plasma-assisted strategies incorporate plasma processes in combination with other surface modification techniques. Plasma coating can serve as a drug-eluting reservoir and diffusion barrier. The plasma-functionalized surface can serve as a platform for grafting antibacterial agents, and plasma surface activation can improve the adhesion of polymeric layers with antibacterial properties. This article critically reviews plasma-based strategies reported in the recent literature for the development of antibacterial biomaterial surfaces. Studies using both atmospheric and low-pressure plasmas are included in this review. The findings are discussed in terms of the trends in material and precursor selection, modification stability, antibacterial efficacy, the choice of bacterial strains tested, cell culture findings, critical aspects of in vitro performance testing and in vivo experimental design.</p></div>","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":"131 ","pages":"Article 112474"},"PeriodicalIF":7.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0928493121006147/pdfft?md5=1460ad51a21209fc7e3403554c2ce7fd&pid=1-s2.0-S0928493121006147-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39799091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 17
Engineering chitosan nano-cocktail containing iron oxide and ceria: A two-in-one approach for treatment of inflammatory diseases and tracking of material delivery 含有氧化铁和二氧化铈的工程壳聚糖纳米鸡尾酒:治疗炎症性疾病和跟踪物质输送的二合一方法
IF 7.9 1区 工程技术 Q1 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2021-12-01 DOI: 10.1016/j.msec.2021.112477
Yuao Wu , Gary Cowin , Shehzahdi S. Moonshi , Huong D.N. Tran , Najma Annuria Fithri , Andrew K. Whittaker , Run Zhang , Hang T. Ta

In this study, modular two-in-one nano-cocktails were synthesised to provide treatment of inflammatory diseases and also enable tracking of their delivery to the disease sites. Chitosan nano-cocktails loaded with treatment module (cerium oxide nanoparticles) and imaging module (iron oxide nanoparticles) were synthesised by electrostatic self-assembly (Chit-IOCO) and ionic gelation method (Chit-TPP-IOCO), respectively. Their MRI capability, anti-inflammatory and anti-fibrosis ability were investigated. Results demonstrated that Chit-IOCO significantly reduced the expression of TNF-α and COX-2, while Chit-TPP-IOCO reduced IL-6 in the LPS-stimulated macrophages RAW264.7. Cytotoxicity studies showed that the nano-cocktails inhibited the proliferation of macrophages. Additionally, Chit-IOCO exhibited higher in vitro MRI relaxivity than Chit-TPP-IOCO, indicating that Chit-IOCO is a better MRI contrast agent in macrophages. It was possible to track the delivery of Chit-IOCO to the inflamed livers of CCl4-treated C57BL/6 mice, demonstrated by a shortened T2 relaxation time of the livers after injecting Chit-IOCO into mice. In vivo anti-inflammatory and blood tests demonstrated that Chit-IOCO reduced inflammation-related proteins (TNF-a, iNOS and Cox-2) and bilirubin in CCl4 treated C57BL/6. Histology images indicated that the nano-cocktails at the treatment doses did not affect the organs of the mice. Importantly, the nano-cocktail reduced fibrosis of CCl4-treated mouse liver. This is the first reported data on the anti-inflammation and anti-fibrosis efficacy of Chit-IOCO in C57BL/6 mouse liver inflammation model. Overall, Chit-IOCO nanoparticles have shown great potential in MR imaging/detecting and treating/therapeutic capabilities for inflammatory diseases.

在这项研究中,模块化的二合一纳米鸡尾酒被合成以提供炎症性疾病的治疗,并且还能够跟踪它们递送到疾病部位。采用静电自组装法(Chit-IOCO)和离子凝胶法(Chit-TPP-IOCO)分别制备了负载处理模块(氧化铈纳米粒子)和成像模块(氧化铁纳米粒子)的壳聚糖纳米鸡尾酒。观察其MRI性能、抗炎和抗纤维化能力。结果表明,在lps刺激的巨噬细胞RAW264.7中,Chit-IOCO可显著降低TNF-α和COX-2的表达,而chit - tmp - ioco可降低IL-6的表达。细胞毒性研究表明,纳米鸡尾酒抑制巨噬细胞的增殖。此外,Chit-IOCO比Chit-TPP-IOCO表现出更高的体外MRI弛豫度,表明Chit-IOCO是巨噬细胞更好的MRI造影剂。可以跟踪Chit-IOCO向ccl4处理的C57BL/6小鼠炎症肝脏的传递,通过将Chit-IOCO注射到小鼠体内后肝脏的T2 松弛时间缩短来证明。体内抗炎和血液试验表明,Chit-IOCO可降低CCl4治疗C57BL/6的炎症相关蛋白(TNF-a、iNOS和Cox-2)和胆红素。组织学图像显示,纳米鸡尾酒在治疗剂量下对小鼠的器官没有影响。重要的是,纳米鸡尾酒减少了ccl4处理小鼠肝脏的纤维化。这是首次报道的Chit-IOCO对C57BL/6小鼠肝脏炎症模型的抗炎和抗纤维化作用的数据。总的来说,Chit-IOCO纳米颗粒在炎症性疾病的磁共振成像/检测和治疗/治疗能力方面显示出巨大的潜力。
{"title":"Engineering chitosan nano-cocktail containing iron oxide and ceria: A two-in-one approach for treatment of inflammatory diseases and tracking of material delivery","authors":"Yuao Wu ,&nbsp;Gary Cowin ,&nbsp;Shehzahdi S. Moonshi ,&nbsp;Huong D.N. Tran ,&nbsp;Najma Annuria Fithri ,&nbsp;Andrew K. Whittaker ,&nbsp;Run Zhang ,&nbsp;Hang T. Ta","doi":"10.1016/j.msec.2021.112477","DOIUrl":"10.1016/j.msec.2021.112477","url":null,"abstract":"<div><p>In this study, modular two-in-one nano-cocktails were synthesised to provide treatment of inflammatory diseases and also enable tracking of their delivery to the disease sites. Chitosan nano-cocktails loaded with treatment module (cerium oxide nanoparticles) and imaging module (iron oxide nanoparticles) were synthesised by electrostatic self-assembly (Chit-IOCO) and ionic gelation method (Chit-TPP-IOCO), respectively. Their MRI capability, anti-inflammatory and anti-fibrosis ability were investigated. Results demonstrated that Chit-IOCO significantly reduced the expression of TNF-α and COX-2, while Chit-TPP-IOCO reduced IL-6 in the LPS-stimulated macrophages RAW264.7. Cytotoxicity studies showed that the nano-cocktails inhibited the proliferation of macrophages. Additionally, Chit-IOCO exhibited higher in vitro MRI relaxivity than Chit-TPP-IOCO, indicating that Chit-IOCO is a better MRI contrast agent in macrophages. It was possible to track the delivery of Chit-IOCO to the inflamed livers of CCl<sub>4</sub>-treated C57BL/6 mice, demonstrated by a shortened T<sub>2</sub><sup>⁎</sup> relaxation time of the livers after injecting Chit-IOCO into mice. In vivo anti-inflammatory and blood tests demonstrated that Chit-IOCO reduced inflammation-related proteins (TNF-a, iNOS and Cox-2) and bilirubin in CCl<sub>4</sub> treated C57BL/6. Histology images indicated that the nano-cocktails at the treatment doses did not affect the organs of the mice. Importantly, the nano-cocktail reduced fibrosis of CCl<sub>4</sub>-treated mouse liver. This is the first reported data on the anti-inflammation and anti-fibrosis efficacy of Chit-IOCO in C57BL/6 mouse liver inflammation model. Overall, Chit-IOCO nanoparticles have shown great potential in MR imaging/detecting and treating/therapeutic capabilities for inflammatory diseases.</p></div>","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":"131 ","pages":"Article 112477"},"PeriodicalIF":7.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0928493121006172/pdfft?md5=70fba70607431d87be8b1b87b408d09a&pid=1-s2.0-S0928493121006172-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39799093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 11
Quercetin loaded liposomes modified with galactosylated chitosan prevent LPS/D-GalN induced acute liver injury 半乳糖化壳聚糖修饰槲皮素脂质体可预防LPS/D-GalN诱导的急性肝损伤
IF 7.9 1区 工程技术 Q1 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2021-12-01 DOI: 10.1016/j.msec.2021.112527
Xinbo Wei , Depeng Yang , Zheng Xing , Chen Zhao , Li Wang , Yubo Fan , Huan Nie , Haifeng Liu

Quercetin (Que) has been proved to have various biological activities, including anti-oxidation, anti-inflammation and anti-virus, showing great potential in liver protection. However, its water insolubility leads to low bioavailability. Therefore, the development of a suitable drug delivery fashion is imminent. In recent years, liposomes have been widely used in the fields of drug delivery and gene transfer thanks to the cell membrane like structure, easy surface-modification and high encapsulation efficiency. Herein, we fabricated Que loaded anionic liposomes. Galactosylated chitosan (GC) was simply attached to the surfaces of liposomes through electrostatic adsorption to achieve targeted delivery by binding to asialoglycoprotein receptor (ASGPR). The results showed that Que loaded liposomes modified with GC (GC-Que-Lipo) could enrich the liver in mice through tail vein injection. Liposomes could achieve sustained drug release and GC-Que-Lipo promoted M2 polarization of macrophages. More importantly, it could maintain low content of AST, ALT, ALP and high level of GSH while reducing lipid oxidation, thereby protecting the liver from damage in acute liver injury model. In general, we expect to be able to acquire targeted and efficient delivery of quercetin through a facile approach, thus fulfill the prevention and treatment of liver diseases.

槲皮素(Que)已被证明具有抗氧化、抗炎、抗病毒等多种生物活性,在肝脏保护方面显示出巨大的潜力。然而,其不溶于水导致生物利用度低。因此,开发一种合适的给药方式迫在眉睫。近年来,脂质体由于具有细胞膜状结构、易于表面修饰、包封效率高等特点,在药物传递和基因转移等领域得到了广泛的应用。在此,我们制备了Que负离子脂质体。半乳糖化壳聚糖(GC)通过静电吸附直接附着在脂质体表面,通过与asialalglyprotein receptor (ASGPR)结合实现靶向递送。结果表明,GC修饰的Que脂质体(GC-Que- lipo)可通过尾静脉注射富集小鼠肝脏。脂质体可实现药物缓释,GC-Que-Lipo可促进巨噬细胞M2极化。更重要的是,它可以维持低AST、ALT、ALP含量和高GSH水平,同时减少脂质氧化,从而保护肝脏免受急性肝损伤模型的损害。总的来说,我们希望能够通过简单的方法获得有针对性和有效的槲皮素递送,从而实现肝脏疾病的预防和治疗。
{"title":"Quercetin loaded liposomes modified with galactosylated chitosan prevent LPS/D-GalN induced acute liver injury","authors":"Xinbo Wei ,&nbsp;Depeng Yang ,&nbsp;Zheng Xing ,&nbsp;Chen Zhao ,&nbsp;Li Wang ,&nbsp;Yubo Fan ,&nbsp;Huan Nie ,&nbsp;Haifeng Liu","doi":"10.1016/j.msec.2021.112527","DOIUrl":"10.1016/j.msec.2021.112527","url":null,"abstract":"<div><p>Quercetin (Que) has been proved to have various biological activities, including anti-oxidation, anti-inflammation and anti-virus, showing great potential in liver protection. However, its water insolubility leads to low bioavailability. Therefore, the development of a suitable drug delivery fashion is imminent. In recent years, liposomes have been widely used in the fields of drug delivery and gene transfer thanks to the cell membrane like structure, easy surface-modification and high encapsulation efficiency. Herein, we fabricated Que loaded anionic liposomes. Galactosylated chitosan (GC) was simply attached to the surfaces of liposomes through electrostatic adsorption to achieve targeted delivery by binding to asialoglycoprotein receptor (ASGPR). The results showed that Que loaded liposomes modified with GC (GC-Que-Lipo) could enrich the liver in mice through tail vein injection. Liposomes could achieve sustained drug release and GC-Que-Lipo promoted M2 polarization of macrophages. More importantly, it could maintain low content of AST, ALT, ALP and high level of GSH while reducing lipid oxidation, thereby protecting the liver from damage in acute liver injury model. In general, we expect to be able to acquire targeted and efficient delivery of quercetin through a facile approach, thus fulfill the prevention and treatment of liver diseases.</p></div>","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":"131 ","pages":"Article 112527"},"PeriodicalIF":7.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0928493121006676/pdfft?md5=b18408ff0dd416b5d8931728fe81496c&pid=1-s2.0-S0928493121006676-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39799097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 14
Corrigendum to “Large-pore-size Ti6Al4V scaffolds with different pore structures for vascularized bone regeneration” [Mater. Sci. Eng. C. 131 (2021) 112499] “具有不同孔隙结构的大孔径Ti6Al4V支架用于血管化骨再生”的更正[Mater.]科学。Eng。C. 131 (2021) 112499]
IF 7.9 1区 工程技术 Q1 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2021-12-01 DOI: 10.1016/j.msec.2021.112531
Chao Wang , Duoling Xu , Ling Lin , Shujun Li , Wentao Hou , Yi He , Liyuan Sheng , Chen Yi , Xiliu Zhang , Hongyu Li , Yiming Li , Wei Zhao , Dongsheng Yu
{"title":"Corrigendum to “Large-pore-size Ti6Al4V scaffolds with different pore structures for vascularized bone regeneration” [Mater. Sci. Eng. C. 131 (2021) 112499]","authors":"Chao Wang ,&nbsp;Duoling Xu ,&nbsp;Ling Lin ,&nbsp;Shujun Li ,&nbsp;Wentao Hou ,&nbsp;Yi He ,&nbsp;Liyuan Sheng ,&nbsp;Chen Yi ,&nbsp;Xiliu Zhang ,&nbsp;Hongyu Li ,&nbsp;Yiming Li ,&nbsp;Wei Zhao ,&nbsp;Dongsheng Yu","doi":"10.1016/j.msec.2021.112531","DOIUrl":"10.1016/j.msec.2021.112531","url":null,"abstract":"","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":"131 ","pages":"Article 112531"},"PeriodicalIF":7.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0928493121006718/pdfft?md5=6006ea7d965ee8b9e72b2c6ff79bbfb2&pid=1-s2.0-S0928493121006718-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39799100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Multifunctional polyethylene imine hybrids decorated by silica bioactive glass with enhanced mechanical properties, antibacterial, and osteogenesis for bone repair 由二氧化硅生物活性玻璃装饰的多功能聚乙烯亚胺杂化物,具有增强的机械性能,抗菌和骨修复成骨功能
IF 7.9 1区 工程技术 Q1 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2021-12-01 DOI: 10.1016/j.msec.2021.112534
Mitra Aghayan, Parvin Alizadeh, Mozhgan Keshavarz

Inorganic/organic hybrids and bioactive glasses demonstrate promising potential as bone substitute biomaterials. A sol-gel hybrid consisting of silica bioactive glass and biodegradable polymer can combine the high bioactivity of a glass with the toughness of a polymer. In this study, multifunctional hybrids with a combination of organic-inorganic hybrid structure class II consisting of polyethyleneimine (PEI) generation 4 (G4) and bioactive glass with enhanced mechanical properties, mineralization, antibacterial, and osteogenesis activities were synthesized by the sol-gel method. Glycidoxypropyl) trimethoxysilane (GPTMS) with different concentrations was used as a covalent bonding agent between PEI polymer and bioactive glass. The effect of GPTMS content was assessed in the presence and absence of calcium in the hybrid structures in terms of morphology, wettability, mechanical properties, antibacterial activity, cell viability, and in vitro osteogenic differentiation properties. By increasing the amount of GPTMS, the compressive strength increased from 1.95 MPa to 2.34 MPa, which was comparable to human trabecular bone. All the hybrids presented antibacterial activity against Staphylococcus aureus, forming an inhibition zone of 13–16 mm. An increase in cell viability of 82.22% in PSCaG90 was obtained after 1 day of MG-63 cell culture. Alkaline phosphatase expression and mineralization of MG-63 cells increased in the PSCaG90 hybrid in the absence of an osteogenic medium compared to PSG60 and PSG90. The PSCaG90 hybrid indicated considerable in vitro osteogenic capacity in the absence of a differentiation medium, expressing high levels of bone-specific proteins including collagen I (COL1A1), Runt-related transcription factor 2 (RUNX2), osteopontin (OPN), and osteocalcin (OCN), compared to calcium-free hybrids. Overall, our results suggest that the presence of calcium in the PSCaG90 leads to a significant increase in osteogenic differentiation of MG-63 cells even in the absence of differentiation medium, which suggests these hybrid structures with multifunctional properties as promising candidates for bone repair.

无机/有机杂合物和生物活性玻璃作为骨替代材料具有广阔的应用前景。由二氧化硅生物活性玻璃和可生物降解聚合物组成的溶胶-凝胶杂化物可以将玻璃的高生物活性与聚合物的韧性结合起来。本研究采用溶胶-凝胶法合成了由聚乙烯亚胺(PEI)第4代(G4)组成的有机-无机杂化结构II类与生物活性玻璃相结合的多功能杂化材料,具有增强的机械性能、矿化、抗菌和成骨活性。用不同浓度的甘氧基丙基三甲氧基硅烷(GPTMS)作为PEI聚合物与生物活性玻璃之间的共价键。从形态学、润湿性、力学性能、抗菌活性、细胞活力和体外成骨分化性能等方面评估了GPTMS含量对杂化结构中钙的存在和不存在的影响。通过增加GPTMS的用量,抗压强度从1.95 MPa增加到2.34 MPa,与人小梁骨相当。所有杂交种均表现出对金黄色葡萄球菌的抑菌活性,形成13 ~ 16 mm的抑菌带。MG-63细胞培养1 d后,PSCaG90细胞活力提高82.22%。与PSG60和PSG90相比,在没有成骨培养基的情况下,PSCaG90中MG-63细胞的碱性磷酸酶表达和矿化增加。PSCaG90杂种在没有分化培养基的情况下显示出相当大的体外成骨能力,与无钙杂种相比,表达高水平的骨特异性蛋白,包括胶原I (COL1A1)、矮子相关转录因子2 (RUNX2)、骨桥蛋白(OPN)和骨钙素(OCN)。总的来说,我们的研究结果表明,即使在没有分化培养基的情况下,PSCaG90中钙的存在也会导致MG-63细胞成骨分化的显著增加,这表明这些具有多功能特性的杂交结构是骨修复的有希望的候选者。
{"title":"Multifunctional polyethylene imine hybrids decorated by silica bioactive glass with enhanced mechanical properties, antibacterial, and osteogenesis for bone repair","authors":"Mitra Aghayan,&nbsp;Parvin Alizadeh,&nbsp;Mozhgan Keshavarz","doi":"10.1016/j.msec.2021.112534","DOIUrl":"10.1016/j.msec.2021.112534","url":null,"abstract":"<div><p>Inorganic/organic hybrids and bioactive glasses demonstrate promising potential as bone substitute biomaterials. A sol-gel hybrid consisting of silica bioactive glass and biodegradable polymer can combine the high bioactivity of a glass with the toughness of a polymer. In this study, multifunctional hybrids with a combination of organic-inorganic hybrid structure class II consisting of polyethyleneimine (PEI) generation 4 (G4) and bioactive glass with enhanced mechanical properties, mineralization, antibacterial, and osteogenesis activities were synthesized by the sol-gel method. Glycidoxypropyl) trimethoxysilane (GPTMS) with different concentrations was used as a covalent bonding agent between PEI polymer and bioactive glass. The effect of GPTMS content was assessed in the presence and absence of calcium in the hybrid structures in terms of morphology, wettability, mechanical properties, antibacterial activity, cell viability, and in vitro osteogenic differentiation properties. By increasing the amount of GPTMS, the compressive strength increased from 1.95 MPa to 2.34 MPa, which was comparable to human trabecular bone. All the hybrids presented antibacterial activity against <em>Staphylococcus aureus</em>, forming an inhibition zone of 13–16 mm. An increase in cell viability of 82.22% in PSCaG90 was obtained after 1 day of MG-63 cell culture. Alkaline phosphatase expression and mineralization of MG-63 cells increased in the PSCaG90 hybrid in the absence of an osteogenic medium compared to PSG60 and PSG90. The PSCaG90 hybrid indicated considerable in vitro osteogenic capacity in the absence of a differentiation medium, expressing high levels of bone-specific proteins including collagen I (COL1A1), Runt-related transcription factor 2 (RUNX2), osteopontin (OPN), and osteocalcin (OCN), compared to calcium-free hybrids. Overall, our results suggest that the presence of calcium in the PSCaG90 leads to a significant increase in osteogenic differentiation of MG-63 cells even in the absence of differentiation medium, which suggests these hybrid structures with multifunctional properties as promising candidates for bone repair.</p></div>","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":"131 ","pages":"Article 112534"},"PeriodicalIF":7.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0928493121006743/pdfft?md5=08042551454ca03f871575c52a8ad3f5&pid=1-s2.0-S0928493121006743-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39799102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Dual-functional antibacterial and osteogenic nisin-based layer-by-layer coatings 具有抗菌和成骨双重功能的肽基逐层涂层
IF 7.9 1区 工程技术 Q1 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2021-12-01 DOI: 10.1016/j.msec.2021.112479
Charlotte Roupie , Béatrice Labat , Sandrine Morin-Grognet , Aline Echalard , Guy Ladam , Pascal Thébault

Implanted biomaterials can be regarded in a cornerstone in the domain of bone surgery. Their surfaces are expected to fulfil two particular requirements: preventing the settlement and the development of bacteria, and stimulating bone cells in view to foster osseointegration. Therefore, a modern approach consists in the design of dual functional coatings with both antibacterial and osteogenic features. To this end, we developed ultrathin Layer-by-Layer (LbL) coatings composed of biocompatible polyelectrolytes, namely chondroitin sulfate A (CSA) and poly-l-lysine (PLL). The coatings were crosslinked with genipin (GnP), a natural and biocompatible crosslinking agent, to increase their resistance against environmental changes, and to confer them adequate mechanical properties with regards to bone cell behaviors. Antibacterial activity was obtained with nisin Z, an antimicrobial peptide (AMP), which is active against gram-positive bacteria. The coatings had a significant bactericidal impact upon Staphylococcus aureus, with fully maintained bone cell adhesion, proliferation and osteogenic differentiation.

植入式生物材料是骨外科领域的基石。它们的表面有望满足两个特殊的要求:防止细菌的沉降和发展,以及刺激骨细胞以促进骨整合。因此,现代方法包括设计具有抗菌和成骨功能的双重功能涂层。为此,我们开发了由生物相容性聚电解质,即硫酸软骨素A (CSA)和聚赖氨酸(PLL)组成的超薄层逐层(LbL)涂层。涂层与天然的生物相容性交联剂genipin (GnP)交联,以增加其对环境变化的抵抗力,并赋予其与骨细胞行为相关的足够的机械性能。用抗菌肽(AMP) nisin Z对革兰氏阳性菌具有抑菌活性。该涂层对金黄色葡萄球菌具有显著的杀菌作用,能充分维持骨细胞的粘附、增殖和成骨分化。
{"title":"Dual-functional antibacterial and osteogenic nisin-based layer-by-layer coatings","authors":"Charlotte Roupie ,&nbsp;Béatrice Labat ,&nbsp;Sandrine Morin-Grognet ,&nbsp;Aline Echalard ,&nbsp;Guy Ladam ,&nbsp;Pascal Thébault","doi":"10.1016/j.msec.2021.112479","DOIUrl":"10.1016/j.msec.2021.112479","url":null,"abstract":"<div><p>Implanted biomaterials can be regarded in a cornerstone in the domain of bone surgery. Their surfaces are expected to fulfil two particular requirements: preventing the settlement and the development of bacteria, and stimulating bone cells in view to foster osseointegration. Therefore, a modern approach consists in the design of dual functional coatings with both antibacterial and osteogenic features. To this end, we developed ultrathin Layer-by-Layer (LbL) coatings composed of biocompatible polyelectrolytes, namely chondroitin sulfate A (CSA) and poly-<span>l</span>-lysine (PLL). The coatings were crosslinked with genipin (GnP), a natural and biocompatible crosslinking agent, to increase their resistance against environmental changes, and to confer them adequate mechanical properties with regards to bone cell behaviors. Antibacterial activity was obtained with nisin Z, an antimicrobial peptide (AMP), which is active against gram-positive bacteria. The coatings had a significant bactericidal impact upon <em>Staphylococcus aureus</em>, with fully maintained bone cell adhesion, proliferation and osteogenic differentiation.</p></div>","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":"131 ","pages":"Article 112479"},"PeriodicalIF":7.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0928493121006196/pdfft?md5=e6289e0afeb31fb11c3a6b3e2ba15e8b&pid=1-s2.0-S0928493121006196-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39686026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
A polydopamine-assisted strontium-substituted apatite coating for titanium promotes osteogenesis and angiogenesis via FAK/MAPK and PI3K/AKT signaling pathways 多多巴胺辅助的锶取代磷灰石涂层通过FAK/MAPK和PI3K/AKT信号通路促进骨生成和血管生成
IF 7.9 1区 工程技术 Q1 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2021-12-01 DOI: 10.1016/j.msec.2021.112482
Yiting Sun , Yaxin Li , Yu Zhang , Tiange Wang , Kaili Lin , Jiaqiang Liu

Early osteointegration is essential for biomedical implants. Surface modifications can significantly compensate for an implant's lack of biocompatibility and osteo-differentiation. They can also be designed to promote angiogenesis in order to assist osteogenesis and ultimately facilitate bone regeneration. In this study, a polydopamine-assisted strontium-substituted apatite coating (Ti@PDA + SrHA) was fabricated on a multifunctional titanium implant to induce both angiogenic and osteogenic abilities for rapid osseointegration. Polydopamine and Sr-substituted hydroxyapatite were coated on the implant through biomineralization. The in vitro results showed that Ti@PDA + SrHA improved cell adhesion and increased the proliferation of rat bone marrow-derived mesenchymal stem cells (rBMSCs) and human umbilical vein endothelial cells (HUVECs). Ti@PDA + SrHA upregulated the expression of ALP activity and osteogenic genes in rBMSCs and elevated angiogenic genes in both rBMSCs and HUVECs. Mechanically, the FAK/MAPK signaling pathway was activated in rBMSCs, and the PI3K/AKT signaling pathway was activated in both rBMSCs and HUVECs. Consistent with these findings, Ti@PDA + SrHA accelerated new bone formation and rapid osseointegration in the femoral condyle implantation study with good stability. Overall, we fabricated a multifunctional biocompatible implant with better angiogenic and osteogenic performance compared to the non-coated implant.

早期骨整合对生物医学植入物至关重要。表面修饰可以显著弥补种植体生物相容性和骨分化的不足。它们也可以被设计成促进血管生成,以帮助成骨,最终促进骨再生。在这项研究中,在多功能钛种植体上制备了一种聚多巴胺辅助锶取代磷灰石涂层(Ti@PDA + SrHA),以诱导血管生成和成骨能力,实现快速骨整合。聚多巴胺和sr取代羟基磷灰石通过生物矿化涂覆在种植体上。体外实验结果显示,Ti@PDA + SrHA可改善大鼠骨髓间充质干细胞(rBMSCs)和人脐静脉内皮细胞(HUVECs)的细胞粘附,促进细胞增殖。Ti@PDA + SrHA上调rBMSCs中ALP活性和成骨基因的表达,上调rBMSCs和huvec中血管生成基因的表达。机械地,FAK/MAPK信号通路在rBMSCs中被激活,PI3K/AKT信号通路在rBMSCs和huvec中都被激活。与这些发现一致,Ti@PDA + SrHA在股骨髁植入研究中加速新骨形成和快速骨整合,具有良好的稳定性。总的来说,我们制造了一种多功能生物相容性种植体,与无涂层种植体相比,它具有更好的血管生成和成骨性能。
{"title":"A polydopamine-assisted strontium-substituted apatite coating for titanium promotes osteogenesis and angiogenesis via FAK/MAPK and PI3K/AKT signaling pathways","authors":"Yiting Sun ,&nbsp;Yaxin Li ,&nbsp;Yu Zhang ,&nbsp;Tiange Wang ,&nbsp;Kaili Lin ,&nbsp;Jiaqiang Liu","doi":"10.1016/j.msec.2021.112482","DOIUrl":"10.1016/j.msec.2021.112482","url":null,"abstract":"<div><p>Early osteointegration is essential for biomedical implants. Surface modifications can significantly compensate for an implant's lack of biocompatibility and osteo-differentiation. They can also be designed to promote angiogenesis in order to assist osteogenesis and ultimately facilitate bone regeneration. In this study, a polydopamine-assisted strontium-substituted apatite coating (Ti@PDA + SrHA) was fabricated on a multifunctional titanium implant to induce both angiogenic and osteogenic abilities for rapid osseointegration. Polydopamine and Sr-substituted hydroxyapatite were coated on the implant through biomineralization. The <em>in vitro</em> results showed that Ti@PDA + SrHA improved cell adhesion and increased the proliferation of rat bone marrow-derived mesenchymal stem cells (rBMSCs) and human umbilical vein endothelial cells (HUVECs). Ti@PDA + SrHA upregulated the expression of ALP activity and osteogenic genes in rBMSCs and elevated angiogenic genes in both rBMSCs and HUVECs. Mechanically, the FAK/MAPK signaling pathway was activated in rBMSCs, and the PI3K/AKT signaling pathway was activated in both rBMSCs and HUVECs. Consistent with these findings, Ti@PDA + SrHA accelerated new bone formation and rapid osseointegration in the femoral condyle implantation study with good stability. Overall, we fabricated a multifunctional biocompatible implant with better angiogenic and osteogenic performance compared to the non-coated implant.</p></div>","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":"131 ","pages":"Article 112482"},"PeriodicalIF":7.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0928493121006226/pdfft?md5=77c9b2e8106cdfdd127863539990c94f&pid=1-s2.0-S0928493121006226-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39686029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 25
Fast dissolving nanofiber mat for the local antimicrobial application of roxithromycin in oral cavity 快速溶解纳米纤维垫在罗红霉素口腔局部抗菌中的应用
IF 7.9 1区 工程技术 Q1 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2021-12-01 DOI: 10.1016/j.msec.2021.112537
Sungyun Kim , Jung-Jin Kim , Jae-Hyeon Jeong , Yonghoon Kim , JiHye Park , Da In Jeong , Hyun Jin Kim , ChaeRim Hwang , Sung-Hoon Ahn , Hyun-Jeong Ko , Hyun-Jong Cho

Fast disintegrating and dissolving nanofiber (NF) mat was devised to deliver roxithromycin for the treatment of the respiratory tract infection. NF membrane was made by an electrospinning process with poly(vinyl alcohol) (PVA), hydroxypropyl-β-cyclodextrin (HP-β-CD), and d-α-tocopheryl polyethylene glycol succinate (TPGS) for local application of roxithromycin. Roxithromycin has a poor water solubility thus HP-β-CD is introduced for enhancing drug solubility by forming an inclusion complex in this study. The addition of TPGS provided multiple roles such as accelerating wetting, disintegration, and dissolution speed and overcoming bacterial resistance. Roxithromycin was successfully entrapped in NF structure and drug amorphization occurred during the electrospinning process. PVA/HP-β-CD/TPGS/roxithromycin (PHTR) NF exhibited faster wetting, disintegration, and dissolution speed rather than the other NF mats. PHTR NF displayed higher antibacterial potentials in Gram-negative bacteria (E. coli) and Gram-positive bacteria (S. aureus) compared to other NF mat formulations. The administration of PHTR NF to oral cavity in pneumococcal disease mouse model provided the most efficient therapeutic potentials in lung tissue. Designed multiple phase-based NF mat may be one of powerful local drug delivery systems for the therapy of respiratory tract infection.

设计了快速崩解溶解纳米纤维(NF)垫,用于递送罗红霉素治疗呼吸道感染。以聚乙烯醇(PVA)、羟丙基-β-环糊精(HP-β-CD)和d-α-生育酚基聚乙二醇琥珀酸酯(TPGS)为原料,采用静电纺丝法制备了局部应用罗红霉素的纳滤膜。罗红霉素的水溶性较差,因此本研究引入HP-β-CD通过形成包合物来提高药物的溶解度。TPGS的加入具有加速润湿、崩解和溶解速度以及克服细菌耐药性等多种作用。罗红霉素被成功地包裹在NF结构中,在静电纺丝过程中发生了药物非晶化。PVA/HP-β-CD/TPGS/roxithromycin (PHTR) NF具有较快的润湿、崩解和溶出速度。PHTR NF对革兰氏阴性菌(大肠杆菌)和革兰氏阳性菌(金黄色葡萄球菌)的抑菌效果优于其他NF NF制剂。在肺炎球菌病小鼠模型中,PHTR NF口腔给药在肺组织中提供了最有效的治疗潜力。设计的多相NF垫可能是治疗呼吸道感染的一种有效的局部给药系统。
{"title":"Fast dissolving nanofiber mat for the local antimicrobial application of roxithromycin in oral cavity","authors":"Sungyun Kim ,&nbsp;Jung-Jin Kim ,&nbsp;Jae-Hyeon Jeong ,&nbsp;Yonghoon Kim ,&nbsp;JiHye Park ,&nbsp;Da In Jeong ,&nbsp;Hyun Jin Kim ,&nbsp;ChaeRim Hwang ,&nbsp;Sung-Hoon Ahn ,&nbsp;Hyun-Jeong Ko ,&nbsp;Hyun-Jong Cho","doi":"10.1016/j.msec.2021.112537","DOIUrl":"10.1016/j.msec.2021.112537","url":null,"abstract":"<div><p>Fast disintegrating and dissolving nanofiber (NF) mat was devised to deliver roxithromycin for the treatment of the respiratory tract infection. NF membrane was made by an electrospinning process with poly(vinyl alcohol) (PVA), hydroxypropyl-β-cyclodextrin (HP-β-CD), and d-α-tocopheryl polyethylene glycol succinate (TPGS) for local application of roxithromycin. Roxithromycin has a poor water solubility thus HP-β-CD is introduced for enhancing drug solubility by forming an inclusion complex in this study. The addition of TPGS provided multiple roles such as accelerating wetting, disintegration, and dissolution speed and overcoming bacterial resistance. Roxithromycin was successfully entrapped in NF structure and drug amorphization occurred during the electrospinning process. PVA/HP-β-CD/TPGS/roxithromycin (PHTR) NF exhibited faster wetting, disintegration, and dissolution speed rather than the other NF mats. PHTR NF displayed higher antibacterial potentials in Gram-negative bacteria (<em>E. coli</em>) and Gram-positive bacteria (<em>S. aureus</em>) compared to other NF mat formulations. The administration of PHTR NF to oral cavity in pneumococcal disease mouse model provided the most efficient therapeutic potentials in lung tissue. Designed multiple phase-based NF mat may be one of powerful local drug delivery systems for the therapy of respiratory tract infection.</p></div>","PeriodicalId":18212,"journal":{"name":"Materials science & engineering. C, Materials for biological applications","volume":"131 ","pages":"Article 112537"},"PeriodicalIF":7.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0928493121006779/pdfft?md5=1d01753907c825bcea473bdeb066fa5a&pid=1-s2.0-S0928493121006779-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39686033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
期刊
Materials science & engineering. C, Materials for biological applications
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1