Materials Today Bio最新文献_第7页

The combination of hydrogels and rutin-loaded black phosphorus nanosheets treats rheumatoid arthritis 水凝胶与负载芦丁的黑磷纳米片的组合可治疗类风湿性关节炎

IF 8.7 1区医学 Q1 ENGINEERING, BIOMEDICAL

Materials Today Bio

Pub Date : 2024-09-27 DOI: 10.1016/j.mtbio.2024.101264

Jing Hou , Shujiang Yin , Runqing Jiao , Wen Chen , Wenjuan Wang , Han Zhang , Zhiyong Liu , Zhenyang Chen , Xing Tian

Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease characterized by inflammation, joint pain, and cartilage degradation. The fluctuating nature of RA often necessitates long-term oral administration of treatment drugs, which can unfortunately lead to adverse effects such as gastrointestinal discomfort and hepatic and renal dysfunction. Therefore, a percutaneous local delivery method for the release of inflammatory modulators in arthritic joints represents a promising therapeutic approach for RA. In this study, we have developed a unique and innovative therapeutic platform (named BP-Rut@Gel). This hydrogel was formulated by incorporating the drug Rutin (Rut) into Black phosphorus nanosheets (BP) and subsequently integrating them within a Hyaluronic Acid (HA) and Polyvinyl Alcohol (PVA) matrix to create a composite hydrogel. Notably, Secondly, photothermal therapy (PTT) under Near-Infrared Irradiation (NIR) and anti-inflammatory drugs synergistically worked together to efficiently quell inflammation and enhance therapeutic effectiveness. Additionally, toxicity experiments have revealed that our synthesized black phosphorus nanosheet composite hydrogel possesses excellent biocompatibility and significantly reduces the inflammatory response in RA joints. Given these remarkable properties, our BP-Rut@Gel hydrogel held significant promise and demonstrated immense clinical potential for the treatment of RA.

类风湿性关节炎（RA）是一种慢性炎症性自身免疫疾病，以炎症、关节疼痛和软骨退化为特征。由于类风湿性关节炎病情起伏不定，通常需要长期口服治疗药物，但不幸的是，这可能会导致胃肠道不适、肝肾功能障碍等不良反应。因此，在关节炎关节中释放炎症调节剂的经皮局部给药方法是一种很有前景的治疗方法。在这项研究中，我们开发了一种独特而创新的治疗平台（名为 BP-Rut@Gel）。这种水凝胶的配制方法是将药物芦丁（Rutin）加入黑磷纳米片（BP），然后将其融入透明质酸（HA）和聚乙烯醇（PVA）基质中，形成一种复合水凝胶。值得注意的是，其次，近红外照射下的光热疗法（PTT）与抗炎药物协同作用，可有效抑制炎症并提高疗效。此外，毒性实验表明，我们合成的黑磷纳米片复合水凝胶具有良好的生物相容性，能显著减轻 RA 关节的炎症反应。鉴于这些卓越的特性，我们的 BP-Rut@Gel 水凝胶在治疗 RA 方面具有重大的前景和巨大的临床潜力。

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引用次数: 0

Non-pathogenic Trojan horse Nissle1917 triggers mitophagy through PINK1/Parkin pathway to discourage colon cancer 非致病特洛伊木马 Nissle1917 通过 PINK1/Parkin 通路触发有丝分裂，阻止结肠癌的发生

IF 8.7 1区医学 Q1 ENGINEERING, BIOMEDICAL

Materials Today Bio

Pub Date : 2024-09-27 DOI: 10.1016/j.mtbio.2024.101273

Yang Wang , Yao Liu , Xiaomin Su , Lili Niu , Nannan Li , Ce Xu , Zanya Sun , Huishu Guo , Shun Shen , Minghua Yu

Bacteria-mediated antitumor therapy has gained widespread attention for its innate tumor-targeting capability and excellent immune activation properties. Nevertheless, the clinical approval of bacterial therapies remains elusive primarily due to the formidable challenge of balancing safety with enhancing in vivo efficacy. In this study, leveraging the probiotic Escherichia coli Nissle1917 (EcN) emerges as a promising approach for colon cancer therapy, offering a high level of safety attributed to its lack of virulence factors and its tumor-targeting potential owing to its obligate anaerobic nature. Specifically, we delineate the erythrocyte (RBC) membrane-camouflaged EcN, termed as Trojan horse EcN@RBC, which triggers apoptosis in tumor cells by mitigating mitochondrial membrane potential (MMP) and subsequently activating the PINK1/Parkin pathway associated with mitophagy. Concurrently, the decline in MMP induced by mitophagy disrupts the mitochondrial permeability transition pore (MPTP), leading to the release of Cytochrome C and subsequent apoptosis induction. Moreover, synergistic effects were observed through the combination of the autophagy activator rapamycin, bolstering the antitumor efficacy in vivo. These findings offer novel insights into probiotic-mediated antitumor mechanisms and underscore the therapeutic potential of EcN@RBC for colon cancer patients.

细菌介导的抗肿瘤疗法因其天生的肿瘤靶向能力和出色的免疫激活特性而受到广泛关注。然而，细菌疗法的临床批准仍然遥遥无期，这主要是由于平衡安全性与提高体内疗效之间的巨大挑战。在这项研究中，利用益生菌大肠杆菌 Nissle1917（EcN）治疗结肠癌是一种很有前景的方法，因为它不含毒力因子，具有高度的安全性，而且由于其必须厌氧的特性，具有靶向肿瘤的潜力。具体来说，我们描述了红细胞（RBC）膜伪装的 EcN（称为特洛伊木马 EcN@RBC），它通过降低线粒体膜电位（MMP）触发肿瘤细胞凋亡，随后激活与有丝分裂相关的 PINK1/Parkin 通路。同时，有丝分裂引起的 MMP 下降会破坏线粒体通透性转换孔（MPTP），导致细胞色素 C 的释放，进而诱导细胞凋亡。此外，通过与自噬激活剂雷帕霉素联合使用，还观察到了协同效应，增强了体内的抗肿瘤功效。这些发现提供了对益生菌介导的抗肿瘤机制的新见解，并强调了 EcN@RBC 对结肠癌患者的治疗潜力。

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引用次数: 0

Magnetic nanobead assisted the dual targets driven fluorescent biosensor based on SPEXPAR and MNAzyme for the olfactory marker protein detection 基于 SPEXPAR 和 MNAzyme 的纳米磁珠辅助双目标驱动荧光生物传感器用于嗅觉标志蛋白检测

IF 8.7 1区医学 Q1 ENGINEERING, BIOMEDICAL

Materials Today Bio

Pub Date : 2024-09-27 DOI: 10.1016/j.mtbio.2024.101272

Jing Qi , Xuemin Cao , Hongyi Bao , Tuodi Zhang , Yichen Wang , Ya Wen , Junling Yang , Guixuan Ge , Ping Wang , Lin Chen , Feng Wang

引用次数: 0

Decellularized extracellular matrix-based disease models for drug screening 用于药物筛选的基于细胞外基质的脱细胞疾病模型

IF 8.7 1区医学 Q1 ENGINEERING, BIOMEDICAL

Materials Today Bio

Pub Date : 2024-09-27 DOI: 10.1016/j.mtbio.2024.101280

Zhoujiang Chen , Ji Wang , Ranjith Kumar Kankala , Mingli Jiang , Lianlin Long , Wei Li , Liang Zou , Aizheng Chen , Ya Liu

In vitro drug screening endeavors to replicate cellular states closely resembling those encountered in vivo, thereby maximizing the fidelity of drug effects and responses within the body. Decellularized extracellular matrix (dECM)-based materials offer a more authentic milieu for crafting disease models, faithfully emulating the extracellular components and structural complexities encountered by cells in vivo. This review discusses recent advancements in leveraging dECM-based materials as biomaterials for crafting cell models tailored for drug screening. Initially, we delineate the biological functionalities of diverse ECM components, shedding light on their potential influences on disease model construction. Further, we elucidate the decellularization techniques and methodologies for fabricating cell models utilizing dECM substrates. Then, the article delves into the research strides made in employing dECM-based models for drug screening across a spectrum of ailments, including tumors, as well as heart, liver, lung, and bone diseases. Finally, the review summarizes the bottlenecks, hurdles, and promising research trajectories associated with the dECM materials for drug screening, alongside their prospective applications in personalized medicine. Together, by encapsulating the contemporary research landscape surrounding dECM materials in cell model construction and drug screening, this review underscores the vast potential of dECM materials in drug assessment and personalized therapy.

体外药物筛选致力于复制与体内细胞状态十分相似的细胞状态，从而最大限度地提高药物在体内作用和反应的真实性。基于脱细胞细胞外基质（dECM）的材料为制作疾病模型提供了更真实的环境，能忠实地模拟细胞外成分和细胞在体内遇到的复杂结构。这篇综述讨论了利用基于 dECM 的材料作为生物材料制作用于药物筛选的细胞模型的最新进展。首先，我们阐述了不同 ECM 成分的生物功能，揭示了它们对疾病模型构建的潜在影响。此外，我们还阐明了利用 dECM 基质制造细胞模型的脱细胞技术和方法。然后，文章深入探讨了利用基于 dECM 的模型进行药物筛选方面取得的研究进展，包括肿瘤、心脏、肝脏、肺部和骨骼疾病。最后，综述总结了用于药物筛选的 dECM 材料的瓶颈、障碍和有前景的研究轨迹，以及它们在个性化医疗中的应用前景。综上所述，本综述概括了围绕 dECM 材料在细胞模型构建和药物筛选方面的当代研究情况，强调了 dECM 材料在药物评估和个性化治疗方面的巨大潜力。

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引用次数: 0

Injectable, oxygen-releasing, thermosensitive hydrogel promotes vascularized bone formation with prolonged oxygen delivery and improved osteoinductivity 可注射、释放氧气、热敏性水凝胶可促进血管化骨形成，延长氧气输送时间，提高骨诱导性

IF 8.7 1区医学 Q1 ENGINEERING, BIOMEDICAL

Materials Today Bio

Pub Date : 2024-09-27 DOI: 10.1016/j.mtbio.2024.101267

Yixin Xu , Shaowei Zheng , Zinan Tang , Qiang Zhong , Rong Chen , Pinkai Wang , Jinlang Fu , Jiajun Xie , Yanhong Ning , Mingyuan Lei , Ding Wang , Huaming Mai , Hao Li , Chunhan Sun , Zhanjun Shi , Hao Cheng , Zhe Shi

The failure or delay in healing of critical bone defects is primarily due to early local anoxic conditions and reduced osteogenic activity. In this research, we integrated calcium peroxide (CPO) embedded polycaprolactone (PCL) microspheres and osteoinductive nanoparticles (Hydroxyapatite/Laponite) into a thermosensitive hydrogel (Pluronic F127), thereby formulating an injectable oxygen-releasing osteogenic thermosensitive hydrogel. Notably, the oxygen-releasing microspheres (ORMs) within the composite hydrogel provide stable oxygen release for up to 21 days, ensuring the survival, migration, and bioactivity of both mesenchymal stem cells and endothelial cells under anoxic conditions. Additionally, the composite hydrogel significantly augments the osteogenic potential of bone marrow mesenchymal stem cells by providing a biomimetic microenvironment with the incorporation of nano-hydroxyapatite/laponite. Ultimately, the injectable composite hydrogel successfully stimulated bone regeneration within a cranial defect in a rat model after 8 weeks, with enhanced vascularization and bone quality. The engineered hydrogel provides a minimally invasive approach to stimulate bone regeneration with a sustained oxygen supply and osteogenic microenvironment provision, underlining its potential for treating critical bone defects.

严重骨缺损愈合失败或延迟的主要原因是早期局部缺氧条件和成骨活性降低。在这项研究中，我们将内嵌过氧化钙（CPO）的聚己内酯（PCL）微球和诱导骨生成的纳米颗粒（羟基磷灰石/皂石）整合到热敏性水凝胶（Pluronic F127）中，从而配制出一种可注射的释氧致骨热敏性水凝胶。值得注意的是，复合水凝胶中的释氧微球（ORMs）可提供长达 21 天的稳定释氧，确保间充质干细胞和内皮细胞在缺氧条件下的存活、迁移和生物活性。此外，该复合水凝胶通过加入纳米羟基磷灰石/皂石提供仿生微环境，显著增强了骨髓间充质干细胞的成骨潜能。最终，这种可注射的复合水凝胶在 8 周后成功刺激了大鼠模型颅骨缺损处的骨再生，并增强了血管生成和骨质量。这种工程水凝胶提供了一种微创方法，通过持续供氧和提供成骨微环境来刺激骨再生，从而凸显了它在治疗严重骨缺损方面的潜力。

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引用次数: 0

Cancer cell membrane-coated siRNA-Decorated Au/MnO2 nanosensitizers for synergistically enhanced radio-immunotherapy of breast cancer 用于协同增强乳腺癌放射免疫疗法的癌细胞膜包被 siRNA Decorated Au/MnO2 纳米敏化剂

IF 8.7 1区医学 Q1 ENGINEERING, BIOMEDICAL

Materials Today Bio

Pub Date : 2024-09-26 DOI: 10.1016/j.mtbio.2024.101275

Diyu Wang , Subin Lin , Tuanwei Li , Xiaohu Yang , Xiang Zhong , Qian Chen , Guoqin Jiang , Chunyan Li

Radiotherapy plays a critical role in the clinical treatment of breast cancer. However, the efficacy of traditional X-ray radiotherapy is greatly limited by its low tumor specificity and treatment tolerance mediated by the tumor microenvironment. Herein, we proposed a novel nano-radiotherapy sensitization strategy to design and construct a cancer cell membrane-coated siRNA-decorated Au/MnO₂ nanosensitizer (R&F@Au/MnO₂-CM) to synergistically enhance radio-immunotherapy for breast cancer. In the integrated nanosensitizer, the cancer cell membrane (CM) derived from 4T1 breast cancer cells is utilized for targeted functionality, while Au/MnO₂ is designed to improve X-ray absorption and alleviate tumor hypoxia. Additionally, PD-L1 siRNA (R) is used to downregulate PD-L1 expression in tumor cells. In an in situ mouse model of 4T1 breast cancer, R&F@Au/MnO₂-CM demonstrated accurate tumor identification via CM-mediated homologous targeting after intravenous injection, which was monitored in real-time through NIR-II fluorescence imaging of NIR-935 (F). Subsequently, the radiotherapy sensitivity was achieved due to the strong radiation absorption properties and oxygen generation through catalysis of Au/MnO₂ upon X-ray irradiation. Furthermore, the immunosuppressive microenvironment of the tumor is improved by downregulating PD-L1, enhancing synergistic anti-tumor effect. Our findings demonstrate a promising approach for tumor treatment by combining targeted enhanced radiotherapy with immune activation.

放疗在乳腺癌的临床治疗中起着至关重要的作用。然而，传统的 X 射线放疗因其肿瘤特异性低和肿瘤微环境介导的治疗耐受性而极大地限制了其疗效。在此，我们提出了一种新型的纳米放疗增敏策略，设计并构建了一种癌细胞膜包被siRNA装饰的金/二氧化锰纳米增敏剂（R&F@Au/MnO2-CM），以协同增强乳腺癌的放射免疫治疗。在这种集成纳米增感剂中，从 4T1 乳腺癌细胞中提取的癌细胞膜（CM）被用于靶向功能，而 Au/MnO2 则被设计用于改善 X 射线吸收和缓解肿瘤缺氧。此外，PD-L1 siRNA（R）用于下调肿瘤细胞中 PD-L1 的表达。在 4T1 乳腺癌原位小鼠模型中，R&F@Au/MnO2-CM 在静脉注射后通过 CM 介导的同源靶向作用准确识别了肿瘤，并通过近红外-935（F）的近红外-II 荧光成像进行了实时监测。随后，由于 Au/MnO2 在 X 射线照射下具有很强的辐射吸收特性和催化氧生成特性，实现了放疗敏感性。此外，还通过下调 PD-L1 改善了肿瘤的免疫抑制微环境，增强了协同抗肿瘤效果。我们的研究结果表明，将靶向增强放射治疗与免疫激活相结合是一种很有前景的肿瘤治疗方法。

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引用次数: 0

All-organic transistors printed on a biodegradable and bioderived substrate for sustainable bioelectronics 印制在可生物降解和生物衍生基底上的全有机晶体管，实现可持续生物电子学

IF 8.7 1区医学 Q1 ENGINEERING, BIOMEDICAL

Materials Today Bio

Pub Date : 2024-09-26 DOI: 10.1016/j.mtbio.2024.101274

Fabrizio A. Viola , Ksenija Maksimovic , Pietro Cataldi , Camilla Rinaldi , Elena Stucchi , Filippo Melloni , Athanassia Athanassiou , Mario Caironi

Biodegradable electronics is an incipient need in order to mitigate the alarming increase of electronic waste worldwide caused by capillary penetration of electronic devices and sensors. Flexibility, solution processability, low capital expenditure, and energy-efficient processes, which are distinctive features of organic printed electronics, have to be complemented by a sustainable sourcing and end-of-life of materials employed. This requirement calls for solutions where materials, especially substrates that typically represent the largest volume, can be biodegraded in the environment with no harm, yet assuring that no precious resources are dispersed. In this work, the bioderived and biodegradable biopolymer polyhydroxybutyrate (PHB) was used as a substrate, cast from an acetic acid solution, for all-organic field effect transistors (OFETs) based on an inkjet printed polymer semiconductor. The OFETs showed small device-to-device variation, a proper current modulation with I_ON/I_OFF of about 1.2·10³, mobility values as high as 0.07 cm²/Vs in saturation regime and channel length/width normalized leakage currents in the order of nA, which remained almost unaltered also after intensive mechanical stresses upon bending and rolling. Such mechanical stability and flexibility, together with the biodegradability and bioderivation, make PHB an appealing candidate for the development of sustainable printed bioelectronics, with widespread future applications in the biomedical and food packaging sector.

可生物降解的电子产品是一种新的需求，以缓解电子设备和传感器的毛细渗透造成的全球电子垃圾的惊人增长。灵活性、解决方案的可加工性、低资本支出和高能效工艺是有机印刷电子产品的显著特点，但还必须辅之以可持续的材料采购和报废处理。这一要求要求解决方案中的材料，尤其是体积最大的基材，能够在环境中进行生物降解而不会对人体造成伤害，同时确保不会分散宝贵的资源。在这项研究中，利用生物衍生和可生物降解的生物聚合物聚羟基丁酸酯（PHB）作为基底，从醋酸溶液中浇铸出基于喷墨打印聚合物半导体的全有机场效应晶体管（OFET）。这种场效应晶体管的器件与器件之间的差异很小，具有适当的电流调制（ION/IOFF 约为 1.2-103），在饱和状态下的迁移率值高达 0.07 cm2/Vs，沟道长度/宽度归一化漏电流为 nA 量级，即使在弯曲和滚动过程中承受高强度机械应力后也几乎保持不变。这种机械稳定性和柔韧性以及生物降解性和生物活化性使 PHB 成为开发可持续印刷生物电子学的理想候选材料，未来将广泛应用于生物医学和食品包装领域。

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引用次数: 0

Polydopamine-functionalized calcium-deficient hydroxyapatite 3D-printed scaffold with sustained doxorubicin release for synergistic chemo-photothermal therapy of osteosarcoma and accelerated bone regeneration 具有持续释放多柔比星功能的多巴胺功能化缺钙羟基磷灰石三维打印支架，用于骨肉瘤的协同化疗-光热疗法和加速骨再生

IF 8.7 1区医学 Q1 ENGINEERING, BIOMEDICAL

Materials Today Bio

Pub Date : 2024-09-25 DOI: 10.1016/j.mtbio.2024.101253

Lu Wang , Zihan Dai , Jianqiang Bi , Yunzhen Chen , Ziyu Wang , Zhenqian Sun , Zhongjie Ji , Hongliang Wang , Yan Zhang , Limei Wang , Junjie Mao , Junxing Yang

Interior bone-tissue regeneration and rapid tumor recurrence post-resection are critical challenges in osteosarcoma and other bone cancers. Conventional bone tissue engineering scaffolds lack inhibitory effects on bone tumor recurrence. Herein, multifunctional scaffolds (named DOX/PDA@CDHA) were designed through the spontaneous polymerization of Dopamine (PDA) on the surface of Calcium Deficient Hydroxyapatite (CDHA) scaffolds, followed by in situ loading of the chemotherapeutic drug Doxorubicin (DOX). The PDA coating endowed the scaffolds with significant photothermal properties, while the gradual release of DOX provided an effective chemotherapeutic effect. The on-demand release of DOX at tumor sites, triggered by dual stimulation (near-infrared (NIR) light and the acidic pH typical of tumor microenvironments), specifically targets cancer cells, thereby mitigating systemic side effects. These unique characteristics facilitated effective osteosarcoma eradication both in vitro and in vivo. Moreover, the scaffold's composition, which mimics the mineral phase of natural bone and is enhanced by PDA's biocompatibility, promotes critical osteogenic and angiogenic processes. This facilitates not only tumor eradication but also the regeneration of healthy bone tissue. Collectively, this study presents a potent candidate for the regeneration of bone defects induced by osteosarcoma.

骨组织内部再生和肿瘤切除后的快速复发是骨肉瘤和其他骨癌面临的严峻挑战。传统的骨组织工程支架缺乏对骨肿瘤复发的抑制作用。本文通过在缺钙羟基磷灰石（CDHA）支架表面自发聚合多巴胺（PDA），然后原位负载化疗药物多柔比星（DOX），设计出多功能支架（命名为 DOX/PDA@CDHA）。PDA 涂层赋予了支架显著的光热特性，而 DOX 的逐步释放则提供了有效的化疗效果。在双重刺激（近红外（NIR）光和肿瘤微环境中典型的酸性 pH 值）的触发下，DOX 在肿瘤部位按需释放，专门针对癌细胞，从而减轻了全身副作用。这些独特的特性有助于在体外和体内有效根除骨肉瘤。此外，该支架的组成模拟了天然骨骼的矿物相，并通过 PDA 的生物相容性得到了增强，从而促进了关键的成骨和血管生成过程。这不仅有利于消除肿瘤，还有利于健康骨组织的再生。总之，这项研究为骨肉瘤引起的骨缺损再生提供了一种有效的候选物质。

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引用次数: 0

Targeted regulation of autophagy using sorafenib-loaded biomineralization nanoenzyme for enhanced photodynamic therapy of hepatoma 利用装载索拉非尼的生物矿化纳米酶靶向调节自噬，增强肝癌的光动力疗法

IF 8.7 1区医学 Q1 ENGINEERING, BIOMEDICAL

Materials Today Bio

Pub Date : 2024-09-24 DOI: 10.1016/j.mtbio.2024.101270

Tianming Lu , Zixian Liu , Ruoning Qian , Yitian Zhou , Jun Li , Qiang Zhang , Hao Yang , Wenli Lu , Yanlin Xin , Zejuan Xie , Lesan Yan , Shanshan Wang , Ruogu Qi , Zhengguang Zhang

Sorafenib (SF), a multi-targeted tyrosine kinase inhibitor, serves as a primary therapeutic modality for advanced liver cancer. Nonetheless, its clinical efficacy is hindered by various obstacles, such as limited bioavailability and inadequate accumulation. This study introduces a novel biomimetic mineralization enzyme, known as BSA@Pt/Ce6/SF@M (PCFM). The PCFM incorporates platinum (Pt) as a catalytic agent, SF as a molecular-targeted therapeutic agent, and Ce6 as a photosensitizer within liver cancer cell membranes. This strategy enables the combination of various anti-tumor treatments, such as photodynamic therapy (PDT) and autophagy induction, leading to increased bioavailability of SF and achieving a multidimensional synergistic anticancer effect. The PDT effect produced by Ce6 in PCFM greatly enhances SF-induced autophagy, effectively promoting autophagic cell death. Furthermore, Pt dissociates from the biomineralization process, acquiring peroxidase properties through chemokinetic reactions. This facilitates the catalysis of significant oxygen generation, addressing the challenge of hypoxia in the tumor microenvironment and improving the efficacy of PDT. Moreover, the SF further enhances therapeutic efficacy by inducing autophagy in response to energy deprivation, as indicated by the reduced levels of HIF-1α, p62, along with increased levels of ROS and LC3-Ⅱ/Ι. This biomineralization-based nanoenzyme exhibits strong anti-tumor characteristics, offering a novel strategy for overcoming challenges in liver cancer treatment.

索拉非尼（Sorafenib）是一种多靶点酪氨酸激酶抑制剂，是晚期肝癌的主要治疗方法。然而，其临床疗效却受到生物利用度有限和蓄积不足等各种障碍的阻碍。本研究介绍了一种新型仿生矿化酶，即 BSA@Pt/Ce6/SF@M（PCFM）。PCFM 将铂 (Pt) 作为催化剂、SF 作为分子靶向治疗剂以及 Ce6 作为光敏剂结合到肝癌细胞膜中。这种策略能够将光动力疗法（PDT）和自噬诱导等多种抗肿瘤疗法结合起来，从而提高 SF 的生物利用率，实现多维协同抗癌效果。PCFM 中的 Ce6 产生的光动力疗法效应大大增强了 SF 诱导的自噬作用，有效促进了细胞的自噬死亡。此外，铂在生物矿化过程中解离，通过趋化反应获得过氧化物酶特性。这有利于催化大量氧气的生成，从而解决肿瘤微环境缺氧的难题，提高光动力疗法的疗效。此外，SF 还能通过诱导自噬来响应能量剥夺，从而进一步提高疗效，HIF-1α 和 p62 水平的降低以及 ROS 和 LC3-Ⅱ/Ι 水平的升高就说明了这一点。这种基于生物矿化的纳米酶具有很强的抗肿瘤特性，为克服肝癌治疗难题提供了一种新策略。

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引用次数: 0

Amorphous zinc phosphate nanoclusters loaded polycarbonate thermosensitive hydrogel: An innovative strategy for promoting wound healing 无定形磷酸锌纳米团簇负载聚碳酸酯热敏水凝胶：促进伤口愈合的创新策略

IF 8.7 1区医学 Q1 ENGINEERING, BIOMEDICAL

Materials Today Bio

Pub Date : 2024-09-24 DOI: 10.1016/j.mtbio.2024.101266

Siwen Chen , Yutong Li , Sihang Ren , Yuanyuan Yang , Zhipeng Hou , Siyu Han , Wanhong Zhang , Jing Guo , Jianshe Hu , Xing Zhang , Liqun Yang

Skin trauma is a matter of great concern for public health, emphasizing the importance of reconstructing the microenvironment at the trauma site to facilitate tissue regeneration. Therefore, the investigation of innovative wound dressings has significant research and clinical implications. In this study, we prepared a thermosensitive hydrogel based on a hydrophilic-hydrophobic-hydrophilic triblock polycarbonate polymer (PTP), and created a composite hydrogel, PTPH-AZP, by incorporating amorphous zinc phosphate (AZP) nanoclusters. We evaluated the effects of PTPH-AZP on human umbilical vein endothelial cells (HUVECs) and the ability to promote skin wound healing. According to the results, PTPH-AZP was found to promote the proliferation, migration, and tube formation of HUVECs through the sustained release of Zn²⁺ at appropriate concentrations. In vivo experiments demonstrated that in the early-mid stages of wound healing, PTPH-AZP promotes increases in Platelet Endothelial Cell Adhesion Molecule-1 (CD31) and α-Smooth Muscle Actin (α-SMA) content within the wound area, facilitating accelerated re-epithelialization and enhanced collagen deposition. In later healing stages, epidermal thickness in the PTPH-AZP treated group was significantly improved, aligning with surrounding intact skin with no instances of attenuated or hypertrophic scarring observed. The findings from the in vivo study suggested that PTPH-AZP may have a positive impact on vascularization and wound healing. In conclusion, this study presents a promising strategy for skin wound healing, highlighting the potential of PTPH-AZP as an effective therapeutic approach.

皮肤创伤是一个备受公众健康关注的问题，它强调了重建创伤部位微环境以促进组织再生的重要性。因此，研究创新型伤口敷料具有重要的研究和临床意义。在这项研究中，我们制备了一种基于亲水-疏水-亲水三嵌段聚碳酸酯聚合物（PTP）的热敏水凝胶，并通过加入无定形磷酸锌（AZP）纳米团簇制备了一种复合水凝胶 PTPH-AZP。我们评估了 PTPH-AZP 对人脐静脉内皮细胞（HUVECs）的影响以及促进皮肤伤口愈合的能力。结果发现，PTPH-AZP 在适当浓度下可通过持续释放 Zn2+ 促进 HUVEC 的增殖、迁移和管形成。体内实验表明，在伤口愈合的早期和中期阶段，PTPH-AZP 可促进伤口区域内血小板内皮细胞粘附分子-1（CD31）和α-平滑肌肌动蛋白（α-SMA）含量的增加，从而加速伤口的再上皮化和胶原沉积。在后期愈合阶段，PTPH-AZP 治疗组的表皮厚度明显改善，与周围完整皮肤一致，没有观察到衰减或增生性疤痕。体内研究结果表明，PTPH-AZP 可能对血管生成和伤口愈合有积极影响。总之，这项研究提出了一种很有前景的皮肤伤口愈合策略，凸显了 PTPH-AZP 作为一种有效治疗方法的潜力。

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