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Smart drug delivery and responsive microneedles for wound healing 用于伤口愈合的智能药物输送和反应微针
IF 8.7 1区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-10-29 DOI: 10.1016/j.mtbio.2024.101321
Meixuan Liu , Jing Jiang , Yiran Wang , Huan Liu , Yiping Lu , Xingang Wang
Wound healing is an ongoing concern for the medical community. The limitations of traditional dressings are being addressed by materials and manufacturing technology. Microneedles (MNs) are a novel type of drug delivery system that has been widely used in cancer therapy, dermatological treatment, and insulin and vaccine delivery. MNs locally penetrate necrotic tissue, eschar, biofilm and epidermis into deep tissues, avoiding the possibility of drug dilution and degradation and greatly improving administration efficiency with less pain. MNs represent a new direction for wound treatment and transdermal delivery. In this study, we summarise the skin wound healing process and the mechanical stimulation of MNs in the context of the wound healing process. We also introduce the structural design and manufacture of MNs. Subsequently, MNs are categorised according to the loaded drugs, where the design of the MNs according to the traumatic biological/biochemical microenvironment (pH, glucose, and bacteria) and the physical microenvironment (temperature, light, and ultrasound) is emphasised. Finally, the advantages of MNs are compared with traditional drug delivery systems and their prospects are discussed.
伤口愈合是医学界一直关注的问题。材料和制造技术正在解决传统敷料的局限性。微针(MNs)是一种新型给药系统,已广泛应用于癌症治疗、皮肤病治疗、胰岛素和疫苗给药。微针可从局部穿透坏死组织、焦痂、生物膜和表皮,进入深层组织,避免了药物稀释和降解的可能性,大大提高了给药效率,并减少了疼痛。MNs 代表了伤口治疗和透皮给药的新方向。在本研究中,我们总结了皮肤伤口愈合过程以及 MNs 在伤口愈合过程中的机械刺激。我们还介绍了 MN 的结构设计和制造。随后,我们根据装载的药物对 MNs 进行了分类,其中强调了根据创伤生物/生化微环境(pH 值、葡萄糖和细菌)和物理微环境(温度、光线和超声波)对 MNs 进行的设计。最后,比较了 MNs 与传统给药系统的优势,并讨论了其前景。
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引用次数: 0
Catechol-rich gelatin microspheres as restorative medical implants intended for inhibiting seroma formation and promoting wound healing 富含儿茶酚的明胶微球作为修复性医疗植入物,用于抑制血清肿形成和促进伤口愈合
IF 8.7 1区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-10-29 DOI: 10.1016/j.mtbio.2024.101313
Xinping Wang , Guoqing Wang , Jianfei Wang , Junqiang Xue , Gaoli Liu , Changjiang Fan
Seroma formation and poor wound healing are common complications of many surgeries that create anatomical dead space (i.e., mastectomy), often causing tissue infection and even necrosis. Although negative pressure drainage and tissue adhesives are investigated to alleviate fluid accumulation post-surgery, however, their therapeutic efficacy remains unsatisfactory in most cases. Herein, the catechol-rich chemically crosslinked gelatin microspheres (ca-CGMSs) have been developed as biodegradable reconstructive implants for preventing seroma formation and concurrently promoting subcutaneous wound healing. Compared with the most representative hydrogel adhesive, i.e. commercial porcine fibrin sealant (PFS), the loosely packed ca-CGMSs with diameters range from 50 to 350 μm, provide numerous cell-adhesive interfaces and interconnected macro-pores for enhanced cell adhesion, proliferation and migration. Subcutaneous embedding trials show the in situ swelling aggregation and wet tissue adhesion of ca-CGMSs as well as their capacity in recruiting autologous cells in rat mastectomy models. The trials in rabbit mastectomy models demonstrate that, compared with PFS gluing, the implanted dried ca-CGMSs not only significantly inhibit seroma formation, but also achieve enhanced wound healing by inducing the formation of vascularized neo-tissue. The ca-CGMSs show a great potential to be the next-generation of restorative materials for both preventing seroma formation and healing subcutaneous wounds.
血清肿形成和伤口愈合不良是许多造成解剖死腔的手术(如乳房切除术)的常见并发症,通常会导致组织感染甚至坏死。虽然研究人员通过负压引流和组织粘合剂来缓解术后积液,但在大多数情况下,其疗效仍不理想。在此,我们开发了富含儿茶酚的化学交联明胶微球(ca-CGMSs),作为可生物降解的重建植入物,用于防止血清肿形成,同时促进皮下伤口愈合。与最具代表性的水凝胶粘合剂(即商用猪纤维蛋白密封剂(PFS))相比,直径在 50 到 350 μm 之间的松散包装 ca-CGMSs 可提供大量细胞粘合界面和相互连接的大孔,从而增强细胞的粘附、增殖和迁移能力。皮下包埋试验表明,ca-CGMSs 具有原位肿胀聚集和湿组织粘附性,并能在大鼠乳房切除术模型中招募自体细胞。在兔子乳房切除模型中的试验表明,与 PFS 粘合相比,植入的干燥 ca-CGMS 不仅能显著抑制血清肿形成,还能通过诱导血管新生组织的形成促进伤口愈合。ca-CGMS显示出成为新一代修复材料的巨大潜力,既能防止血清肿形成,又能促进皮下伤口愈合。
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引用次数: 0
PEGylated Elesclomol@Cu(Ⅱ)-based Metal‒organic framework with effective nanozyme performance and cuproptosis induction efficacy for enhanced PD-L1-based immunotherapy 基于 PEG 的 Elesclomol@Cu(Ⅱ)金属有机框架具有有效的纳米酶性能和杯突症诱导功效,可用于增强基于 PD-L1 的免疫疗法
IF 8.7 1区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-10-28 DOI: 10.1016/j.mtbio.2024.101317
Xufeng Lu , Wenhai Deng , Shuaibin Wang , Shengsheng Zhao , Bingzi Zhu , Binglong Bai , Yiwen Mao , Ji Lin , Yongdong Yi , Zuoliang Xie , Xiang Wang , Yongyong Lu , Xiufeng Huang , Tao You , Xiaolei Chen , Weijian Sun , Xian Shen
Nanozymes constitute a promising treatment strategy for antitumor therapy. However, the catalytic function of metal‒organic framework (MOF)-based nanozymes during cuproptosis remains unclear. In this study, a Cu(Ⅱ)-based MOF nanocomposite loaded with the copper ionophore elesclomol and surface modified with polyethylene glycol polymer (PEG) was developed (ES@Cu(Ⅱ)-MOF) for effective cuproptosis induction. The peroxidase (POD)-like activity of ES@Cu(Ⅱ)-MOF generated an abundance of hydroxyl radicals (•OH) via a Fenton-like reaction, and its glutathione peroxidase (GSH-Px)-like activity converted Cu2+ into more toxic Cu+ ions to efficiently consume endogenous GSH. Notably, the rapid accumulation of Cu+ and ES in tumor cells induced the aggregation of lipoylated dihydrolipoamide S-acetyltransferase (DLAT) and the downregulation of Fe‒S cluster proteins, ultimately leading to cuproptosis. ES@Cu(Ⅱ)-MOF exhibited extraordinary cytotoxicity against breast cancer cells in vitro and significantly suppressed 4T1 breast tumor growth in vivo. Moreover, ES@Cu(Ⅱ)-MOF induced immunogenic cell death (ICD) to increase the antitumor immune response. Furthermore, combining ES@Cu(Ⅱ)-MOF with an anti-programmed cell death-ligand 1 (PD-L1) antibody converted the immunosuppressive tumor microenvironment to an immunogenic microenvironment, thus effectively inhibiting breast tumor growth. Overall, this work provides an innovative approach utilizing nanozymes to facilitate cuproptosis for cancer treatment, which potentially enhances the effectiveness of immune checkpoint inhibitor-based immunotherapy.
纳米酶是一种很有前景的抗肿瘤治疗策略。然而,基于金属有机框架(MOF)的纳米酶在杯突变过程中的催化功能仍不清楚。本研究开发了一种装载了铜离子诱导剂伊来克洛莫尔并经聚乙二醇聚合物(PEG)表面修饰的基于Cu(Ⅱ)的MOF纳米复合材料(ES@Cu(Ⅱ)-MOF),用于有效诱导杯突酶变。ES@Cu(Ⅱ)-MOF的过氧化物酶(POD)样活性通过芬顿样反应产生大量羟自由基(-OH),其谷胱甘肽过氧化物酶(GSH-Px)样活性将Cu2+转化为毒性更强的Cu+离子,从而有效消耗内源性GSH。值得注意的是,Cu+和ES在肿瘤细胞中的快速积累会诱导脂酰化二氢脂酰胺S-乙酰转移酶(DLAT)的聚集和Fe-S簇蛋白的下调,最终导致杯突症。ES@Cu(Ⅱ)-MOF在体外对乳腺癌细胞具有超强的细胞毒性,在体内能显著抑制4T1乳腺肿瘤的生长。此外,ES@Cu(Ⅱ)-MOF 还能诱导免疫原性细胞死亡(ICD),从而增强抗肿瘤免疫反应。此外,将ES@Cu(Ⅱ)-MOF与抗程序性细胞死亡配体1(PD-L1)抗体结合使用,可将免疫抑制性肿瘤微环境转化为免疫原性微环境,从而有效抑制乳腺肿瘤的生长。总之,这项工作提供了一种利用纳米酶促进杯突酶作用治疗癌症的创新方法,有可能提高基于免疫检查点抑制剂的免疫疗法的有效性。
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引用次数: 0
An intelligent poly aptamer-encoded DNA nanoclew for tumor site activated mitochondria-targeted photodynamic therapy and MR imaging 用于肿瘤部位激活线粒体靶向光动力疗法和核磁共振成像的智能多肽合剂编码 DNA 纳米螯合剂
IF 8.7 1区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-10-28 DOI: 10.1016/j.mtbio.2024.101318
Minghui Chen , Hanbo Xu , Pengzhao Chang , Xueqi Li , Shuang Liu , Lingyue Xu , Kai Xu , Guohui Cheng
Mitochondria-targeted photodynamic therapy (PDT) has emerged as one of the most promising antitumor therapies, as it significantly enhances the efficacy of photosensitizers. An efficient and biocompatible nanocarrier to deliver cationic photosensitizers (PSs) is vital for mitochondria-targeted PDT but still challenging. Herein, a poly-AS1411 aptamer DNA nanoclew (AS-AMD) synthesized via rolling circle amplification (RCA) is developed, incorporating mitochondria-targeted PSs (APNO) and paramagnetic Mn2+ for mitochondria-targeted PDT and magnetic resonance imaging (MRI). The AS1411 aptamer of AS-AMD has been engineered to enhance tumor targeting and cellular internalization. Paramagnetic Mn2+ released in the acidic tumor microenvironment promotes MRI performance of the tumor tissue and guides subsequent PDT. The released cationic APNO selectively targets the mitochondrial membrane and generates reactive oxygen species (ROS) that induce the apoptosis of 4T1 breast tumor cells. Additionally, AS-AMD exhibits effective tumor targeting in the 4T1-tumor-bearing mice model, significantly enhanced MRI performance and PDT efficacy. Therefore, this study introduces an interesting strategy to achieve efficient mitochondrial-targeted delivery of cationic PSs and provides a versatile biocompatible DNA nanoplatform for the development of nanotheranostic agents.
线粒体靶向光动力疗法(PDT)已成为最有前途的抗肿瘤疗法之一,因为它能显著提高光敏剂的疗效。一种高效、生物相容性好的纳米载体可递送阳离子光敏剂(PSs),这对线粒体靶向光动力疗法至关重要,但仍具有挑战性。本文开发了一种通过滚圆扩增(RCA)合成的聚-AS1411适配体DNA纳米载体(AS-AMD),它结合了线粒体靶向光敏剂(APNO)和顺磁性Mn2+,用于线粒体靶向PDT和磁共振成像(MRI)。AS-AMD的AS1411适配体经过设计,可增强肿瘤靶向性和细胞内化。在酸性肿瘤微环境中释放的顺磁性 Mn2+ 可提高肿瘤组织的磁共振成像性能,并指导后续的局部放疗。释放的阳离子 APNO 可选择性地靶向线粒体膜,产生活性氧 (ROS),诱导 4T1 乳腺肿瘤细胞凋亡。此外,AS-AMD 在 4T1 肿瘤小鼠模型中表现出有效的肿瘤靶向性,显著提高了磁共振成像性能和光动力疗法的疗效。因此,本研究提出了一种有趣的策略来实现阳离子 PSs 的高效线粒体靶向递送,并为纳米otheranostic 药剂的开发提供了一种多功能的生物相容性 DNA 纳米平台。
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引用次数: 0
High-performance silk/polylactic acid composite scaffold material with immunomodulation and osteogenesis function 具有免疫调节和成骨功能的高性能丝/聚乳酸复合支架材料
IF 8.7 1区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-10-28 DOI: 10.1016/j.mtbio.2024.101316
Jia Rui , Siyu Zhu , Xiang Xu, Yi Wang, Zulan Liu, Guotao Cheng, Dingpei Long, Lan Cheng, Fangyin Dai
The choice of suitable materials and effective structural design are crucial in influencing the therapeutic outcomes of bone tissue engineering scaffolds. This study introduces a controllable biodegradable composite scaffold composed of flat silkworm cocoon (FSC) and polylactic acid (PLA) as an innovative strategy for promoting bone healing in complex injuries. We focused on optimizing the scaffold's structural design, mechanical properties, and underlying mechanisms of osteogenesis. Initial experiments established the parameters for hot pressing the FSC, followed by mechanical performance tests to identify the optimal preparation conditions. Composite scaffolds incorporating PLA films were subsequently fabricated using these optimized parameters. The results indicate that the FSC/PLA composite scaffold exhibits outstanding biocompatibility, mechanical strength, and in vitro mineralization capabilities, alongside an appropriate degradation rate. Furthermore, the composite scaffolds demonstrated significant potential in promoting osteogenic differentiation and facilitating macrophage polarization toward an anti-inflammatory M2 phenotype. In vivo implantation of the scaffold in defective regions enhanced osteogenesis and mitigated inflammatory responses associated with degradation. This investigation presents an optimal composite scaffold that closely mimics the complex structure of bone, offering a novel approach to enhance bone regeneration and effectively address substantial bone defects.
选择合适的材料和有效的结构设计是影响骨组织工程支架治疗效果的关键。本研究介绍了一种由扁平蚕茧(FSC)和聚乳酸(PLA)组成的可控生物降解复合支架,作为促进复杂损伤骨愈合的创新策略。我们的重点是优化支架的结构设计、机械性能和骨生成的基本机制。最初的实验确定了热压 FSC 的参数,随后进行了机械性能测试,以确定最佳制备条件。随后,利用这些优化参数制作了含有聚乳酸薄膜的复合支架。结果表明,FSC/PLA 复合支架具有出色的生物相容性、机械强度和体外矿化能力,同时降解率也很合适。此外,复合支架在促进成骨分化和促进巨噬细胞向抗炎 M2 表型极化方面表现出了巨大的潜力。在体内将该支架植入有缺陷的区域,可促进成骨,并减轻与降解相关的炎症反应。这项研究提出了一种最佳的复合支架,它能近似模拟骨的复杂结构,为促进骨再生和有效解决实质性骨缺损提供了一种新方法。
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引用次数: 0
Engineering exosomes from fibroblast growth factor 1 pre-conditioned adipose-derived stem cells promote ischemic skin flaps survival by activating autophagy 来自成纤维细胞生长因子 1 预处理脂肪来源干细胞的工程外泌体通过激活自噬促进缺血皮瓣存活
IF 8.7 1区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-10-26 DOI: 10.1016/j.mtbio.2024.101314
Xuanlong Zhang , Xiaoqiong Jiang , Huiming Deng , Gaoxiang Yu , Ningning Yang , Abdullah Al Mamun , Feifei Lian , Tianling Chen , Haijuan Zhang , Yingying Lai , Jiayi Huang , Shi Xu , Fuman Cai , Xiaokun Li , Kailiang Zhou , Jian Xiao

Background

The recovery of ischemic skin flaps is a major concern in clinical settings. The purpose of this study is to evaluate the effects of engineered exosomes derived from FGF1 pre-conditioned adipose-derived stem cells (FEXO) on ischemic skin flaps.

Method

6 patients who suffered from pressure ulcer at stage 4 and underwent skin flaps surgery were recruited in this study to screen the potential targets of ischemic skin flaps in FGF family. FGF1 was co-incubated with adipose stem cells, and ultracentrifugation was applied to extract FEXO. Transcriptome sequencing analysis was used to determine the most effective microRNA in FEXO. Animal skin flaps models were established in our study to verify the effects of FEXO. Immunofluorescence (IF), western blotting (WB) and other molecular strategy were used to evaluate the effects and mechanism of FEXO.

Results

FGF1 was expected to be the therapeutic and diagnostic target of ischemic skin flaps, but there is still some deficiency in rescuing skin flaps. FEXO significantly improved the viability of RPSFs and endothelial cells by inhibiting oxidative stress and alleviating apoptosis and pyroptosis through augmenting autophagy flux. In addition, FEXO inhibited the over-activated inflammation responses. Transcriptome sequencing analysis showed that miR-183-5p was significantly elevated in FEXO, and inhibiting miR-183-5p resulted in impaired protective effects of autophagy in skin flaps. The exosomal miR-183-5p markedly enhanced cell viability, inhibited oxidative stress and alleviated apoptosis and pyroptosis in endothelial cells by targeting GPR137 through Pi3k/Akt/mTOR pathway, indicating that GPR137 could also be a therapeutic target of ischemic skin flap. It was also notabale that FGF1 increased the number of exosomes by upregulating VAMP3, which may be a promising strategy for clinical translation.

Conclusion

FEXO markedly improved the survivial rate of ischemic skin flaps through miR-183-5p/GPR137/Pi3k/Akt/mTOR axis, which would be a promising strategy to rescue ischemic skin flaps.
背景缺血皮瓣的恢复是临床上的一个主要问题。本研究的目的是评估由FGF1预处理脂肪干细胞(FEXO)衍生的工程外泌体对缺血性皮瓣的影响。方法 本研究招募了6名褥疮4期并接受皮瓣手术的患者,以筛选FGF家族中缺血性皮瓣的潜在靶点。将 FGF1 与脂肪干细胞共培养,并采用超速离心法提取 FEXO。利用转录组测序分析确定了 FEXO 中最有效的 microRNA。我们的研究建立了动物皮瓣模型,以验证 FEXO 的效果。结果FGF1被认为是缺血性皮瓣的治疗和诊断靶点,但在挽救皮瓣方面仍存在不足。FEXO通过抑制氧化应激,通过增强自噬通量缓解细胞凋亡和热凋亡,从而明显提高了RPSFs和内皮细胞的活力。此外,FEXO 还能抑制过度激活的炎症反应。转录组测序分析表明,miR-183-5p在FEXO中显著升高,抑制miR-183-5p会导致皮瓣自噬的保护作用受损。外泌体miR-183-5p通过Pi3k/Akt/mTOR通路靶向GPR137,明显增强了细胞活力,抑制了氧化应激,缓解了内皮细胞的凋亡和热凋亡,表明GPR137也可能是缺血性皮瓣的治疗靶点。结论FEXO通过miR-183-5p/GPR137/Pi3k/Akt/mTOR轴显著提高了缺血性皮瓣的存活率,这将是挽救缺血性皮瓣的一种有前途的策略。
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引用次数: 0
Modular iodinated carboxybetaine copolymers as charge-sensitive contrast agents for the detection of cartilage degradation 模块化碘化羧基甜菜碱共聚物作为电荷敏感造影剂用于检测软骨退化
IF 8.7 1区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-10-26 DOI: 10.1016/j.mtbio.2024.101302
Patrick Weber , Annalena Maier , David Fercher, Maryam Asadikorayem, Marcy Zenobi-Wong
Accurately assessing cartilage tissue degradation is a big challenge in osteoarthritis (OA) research, as histology only provides information about a 2D tissue section, and currently available contrast agents for tomographic evaluation suffer from low specificity. In this study, we present a modular platform based on zwitterionic carboxybetaine (CBAA) to create multivalent polymeric contrast agents for x-ray computed tomography (CT) with high specificity towards the anionic glycosaminoglycans in the cartilage tissue. By copolymerizing CBAA with different ratios of anionic and cationic iodinated comonomers, we created a library of polymers with net charges ranging from strongly anionic to strongly cationic. The polymers were applied onto osteochondral plugs with different degradation states and the resulting CT images compared to histological stainings. In healthy tissues, the bulk contrast enhancement was strongly correlated with polymer charge, with cationic polymers reaching a 2-fold stronger contrast compared to established small molecule contrast agents. While a further increase in cationic charge slowed the penetration, it increased the polymer's specificity, thereby enabling the most cationic polymer C40 (40 mol% cationic iodinated comonomer) to discriminate accurately between tissues treated with IL-1β for 0, 1, 2 and 3 weeks. Moreover, this polymer also showed a strong local specificity, visualizing local differences in GAG distribution with significantly increased accuracy compared to the controls. Our polymer contrast agents show the importance of multivalency and charge control for the accurate, volumetric detection of GAGs in the cartilage tissue and paves the way towards new contrast agents in- and outside of the clinic.
准确评估软骨组织退化是骨关节炎(OA)研究中的一大挑战,因为组织学只能提供二维组织切片的信息,而目前可用的用于断层扫描评估的造影剂特异性较低。在这项研究中,我们提出了一种基于齐聚物羧基甜菜碱(CBAA)的模块化平台,用于制造对软骨组织中的阴离子糖胺聚糖具有高特异性的 X 射线计算机断层扫描(CT)用多价聚合物造影剂。通过将 CBAA 与不同比例的阴离子和阳离子碘化共聚单体共聚,我们创建了一个聚合物库,其净电荷从强阴离子到强阳离子不等。我们将这些聚合物应用于不同降解状态的骨软骨塞上,并将所得 CT 图像与组织学染色结果进行比较。在健康组织中,大量对比度增强与聚合物电荷密切相关,阳离子聚合物的对比度比现有的小分子对比剂强 2 倍。虽然阳离子电荷的进一步增加会减慢穿透速度,但却提高了聚合物的特异性,从而使阳离子含量最高的聚合物 C40(阳离子碘化共聚单体含量为 40 摩尔%)能够准确区分经 IL-1β 处理 0 周、1 周、2 周和 3 周的组织。此外,这种聚合物还显示出很强的局部特异性,与对照组相比,它能显示 GAG 分布的局部差异,准确性显著提高。我们的聚合物造影剂显示了多价性和电荷控制对于准确、全面地检测软骨组织中的 GAG 的重要性,并为临床内外的新型造影剂铺平了道路。
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引用次数: 0
Recent advances in coaxial electrospun nanofibers for wound healing 用于伤口愈合的同轴电纺纳米纤维的最新进展
IF 8.7 1区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-10-26 DOI: 10.1016/j.mtbio.2024.101309
Jing Zhao , Liyun Chen , Aiwei Ma , Xujue Bai , Yating Zeng , Daojun Liu , Bo Liu , Wancong Zhang , Shijie Tang
The skin is the body's primary immune barrier, defending it against pathogenic invasion. Skin injuries impose a significant physiological burden on patients, making effective wound management essential. Dressings are commonly employed in wound care, and electrospun nanofiber dressings are a research hotspot owing to their ease of fabrication, cost-effectiveness, and structural similarity to the extracellular matrix. Coaxial electrospinning offers considerable advantages in drug delivery, fiber structure transformation, and enhanced interaction with the host. These attributes make coaxial electrospun materials promising candidates for precision and personalized wound dressings in medical treatments. This review provides a comprehensive overview of wound healing and its influencing factors. It also outlines coaxial electrospinning's production principles and benefits in wound dressings. Guided by the factors affecting wound healing, coaxial electrospun nanofiber dressings have different application modalities. Furthermore, we discuss the current limitations and future directions for enhancing the current coaxial electrospun dressing technologies.
皮肤是人体的主要免疫屏障,可抵御病原体的入侵。皮肤损伤给患者带来了巨大的生理负担,因此有效的伤口管理至关重要。敷料通常用于伤口护理,而电纺纳米纤维敷料因其易于制造、成本效益高以及与细胞外基质结构相似而成为研究热点。同轴电纺在药物输送、纤维结构转换和增强与宿主的相互作用方面具有相当大的优势。这些特性使得同轴电纺材料有望成为医疗中精确和个性化伤口敷料的候选材料。本综述全面概述了伤口愈合及其影响因素。它还概述了同轴电纺的生产原理以及在伤口敷料中的优势。根据影响伤口愈合的因素,同轴电纺纳米纤维敷料有不同的应用模式。此外,我们还讨论了当前同轴电纺敷料技术的局限性和未来改进方向。
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引用次数: 0
PH-sensitive adriamycin hydrochloride and oxaliplatin dual-loaded microspheres synergistically enhance local injections effect of hepatocellular carcinoma PH 敏感盐酸阿霉素和奥沙利铂双载体微球协同增强肝癌局部注射效果
IF 8.7 1区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-10-25 DOI: 10.1016/j.mtbio.2024.101311
Jialu Weng , Shiyi Wu , Yating Pan , Yifan Lai , Jinrong Zhu , Wenzhang Jin , Deyu Lu , Yizhang Chen , Zhijie Yu , Xinjie Zan , Jinglin Xia
Chemotherapy is the primary palliative treatment for advanced hepatocellular carcinoma (HCC). However, the systemic delivery is associated with the drawbacks including a high risk of adverse effects and a low efficacy. Therefore, local injection therapy may be beneficial. Nevertheless, the existing local drug-carrying microspheres(DOBM)have the characteristics of low loading and abrupt release, can not simultaneously load two drugs, and may cause unnecessary toxicity. In this study, we created the dual-loaded bovine serum albumin (BSA) microspheres (also known as DOBM), which were hollow and contained both oxaliplatin (OXA) and Adriamycin hydrochloride (DOX). In addition, this pH-sensitive drug delivery method exhibited a high drug loading capacity and was promising in breaking through biological barriers, making it a viable option for the treatment of HCC through local implantation. Based on physiochemical evaluation of BSA microspheres, they had a porous structure which was close to the surface. Adriamycin and oxaliplatin were successfully added to the surface of BSA microspheres. According to in vitro experimental results, the growth of human HCC (HCC-LM3 and PLC/PRF/5) cell lines was significantly inhibited by DOBM. Furthermore, in the subcutaneous PLC/PRF/5 HCC model, DOBM played an essential role in tumor development and change in the tumor microenvironment.
化疗是晚期肝细胞癌(HCC)的主要姑息治疗方法。然而,全身给药存在不良反应风险高、疗效低等缺点。因此,局部注射疗法可能是有益的。然而,现有的局部载药微球(DOBM)具有载药量低、释放突然的特点,不能同时载入两种药物,而且可能引起不必要的毒性。在这项研究中,我们创造了双载体牛血清白蛋白(BSA)微球(又称 DOBM),它是中空的,同时含有奥沙利铂(OXA)和盐酸阿霉素(DOX)。此外,这种对 pH 值敏感的给药方法具有较高的药物负载能力,并有望突破生物屏障,使其成为通过局部植入治疗 HCC 的可行选择。根据对 BSA 微球的理化评估,它们具有接近表面的多孔结构。阿霉素和奥沙利铂被成功添加到 BSA 微球表面。体外实验结果表明,DOBM 能显著抑制人 HCC(HCC-LM3 和 PLC/PRF/5)细胞株的生长。此外,在皮下 PLC/PRF/5 HCC 模型中,DOBM 对肿瘤的发展和肿瘤微环境的改变起到了至关重要的作用。
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引用次数: 0
Hydrogel local drug delivery systems for postsurgical management of tumors: Status Quo and perspectives 用于肿瘤术后治疗的水凝胶局部给药系统:现状与展望
IF 8.7 1区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-10-24 DOI: 10.1016/j.mtbio.2024.101308
Ziqiao Zhong , Lu Gan , Ziyi Feng , Wenhao Wang , Xin Pan , Chuanbin Wu , Ying Huang
Surgery is one of the primary treatments for solid tumors. However, the incomplete resection of tumor cells and the immunosuppressive microenvironment make the issue of postsurgical tumor recurrence a great challenge. Furthermore, a wide range of requirements, including ensuring effective hemostasis, implementing prophylactic measures against infection, and promoting wound healing, were also raised in the postsurgical management of tumors. To fulfill these demands, multiple hydrogel local drug delivery systems (HLDDS) were developed recently. These HLDDS are expected to offer numerous advantages in the postsurgical management of tumors, such as achieving high local drug concentrations at the lesion, efficient delivery to surgical microcavities, mitigating systemic side effects, and addressing the diverse demand. Thus, in this review, a detailed discussion of the diverse demands of postsurgical management of tumors is provided. And the current publication trend on HLDDS in the postsurgical management of tumors is analyzed and discussed. Then, the applications of different types of HLDDS, in-situ HLDDS and non-in-situ HLDDS, in postsurgical management of tumors were introduced and summarized. Besides, the current problems and future perspectives are discussed. The review is expected to provide an overview of HLDDS in postsurgical management of tumors and promote their clinical application.
手术是实体瘤的主要治疗方法之一。然而,肿瘤细胞的不完全切除和免疫抑制微环境使肿瘤术后复发问题成为一个巨大挑战。此外,肿瘤术后管理还提出了一系列要求,包括确保有效止血、实施预防感染措施和促进伤口愈合。为了满足这些要求,最近开发出了多种水凝胶局部给药系统(HLDDS)。这些水凝胶局部给药系统有望在肿瘤术后治疗中发挥诸多优势,如在病灶处实现高浓度局部给药、向手术微腔高效给药、减轻全身副作用以及满足多样化需求等。因此,本综述详细讨论了肿瘤术后治疗的多样化需求。并分析和讨论了目前 HLDDS 在肿瘤术后治疗中的发表趋势。然后,介绍并总结了不同类型的 HLDDS(原位 HLDDS 和非原位 HLDDS)在肿瘤术后治疗中的应用。此外,还讨论了当前存在的问题和未来展望。本综述旨在概述 HLDDS 在肿瘤术后治疗中的应用,并促进其临床应用。
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引用次数: 0
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Materials Today Bio
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