Pub Date : 2026-01-21DOI: 10.1016/j.mtbio.2026.102811
Yongkang Lai , Yongliang Ouyang , Xiaojing Yin , Tao Yu , Jianhua Wan , Xueyang Li , Yi Hu , Xu Shu , Huan Wang
Chronic pancreatitis (CP) is a lifelong progressive fibrotic inflammatory disorder for which no effective cure is currently available. Persistent and recurrent inflammatory stimulation induced by reactive oxygen species (ROS) is a key driver of pancreatic fibrogenesis, making oxidative stress a promising therapeutic target to halt disease progression. In this study, we developed a nanosystem, HC@CeMOF, consisting of a small-sized cerium-based metal–organic framework (CeMOF) core loaded with curcumin and coated with hyaluronic acid (HA), enabling precise targeting of inflamed pancreatic tissue. HC@CeMOF exhibits a small-sized particle size along with favorable cellular and biological safety profiles. Once administered in vivo, the nanosystem exploits the specific binding affinity of HA to CD44 receptors on macrophages to selectively accumulate at inflamed pancreatic sites. Subsequently, the cerium-based nanozyme efficiently scavenges ROS through the reversible redox cycling between Ce3+ and Ce4+, while the slow release of curcumin further suppresses the NF-κB signaling pathway and modulates inflammatory cytokine levels, thereby achieving synergistic anti-inflammatory and antioxidant effects. Collectively, these mechanisms substantially attenuate CP progression. This targeted ROS-scavenging and anti-inflammatory strategy holds promise as an alternative therapeutic approach for chronic pancreatitis.
{"title":"Engineered targeted Ce-based MOF nanozymes for ROS scavenging and inflammatory Reprogramming in chronic pancreatitis","authors":"Yongkang Lai , Yongliang Ouyang , Xiaojing Yin , Tao Yu , Jianhua Wan , Xueyang Li , Yi Hu , Xu Shu , Huan Wang","doi":"10.1016/j.mtbio.2026.102811","DOIUrl":"10.1016/j.mtbio.2026.102811","url":null,"abstract":"<div><div>Chronic pancreatitis (CP) is a lifelong progressive fibrotic inflammatory disorder for which no effective cure is currently available. Persistent and recurrent inflammatory stimulation induced by reactive oxygen species (ROS) is a key driver of pancreatic fibrogenesis, making oxidative stress a promising therapeutic target to halt disease progression. In this study, we developed a nanosystem, HC@CeMOF, consisting of a small-sized cerium-based metal–organic framework (CeMOF) core loaded with curcumin and coated with hyaluronic acid (HA), enabling precise targeting of inflamed pancreatic tissue. HC@CeMOF exhibits a small-sized particle size along with favorable cellular and biological safety profiles. Once administered <em>in vivo</em>, the nanosystem exploits the specific binding affinity of HA to CD44 receptors on macrophages to selectively accumulate at inflamed pancreatic sites. Subsequently, the cerium-based nanozyme efficiently scavenges ROS through the reversible redox cycling between Ce<sup>3+</sup> and Ce<sup>4+</sup>, while the slow release of curcumin further suppresses the NF-κB signaling pathway and modulates inflammatory cytokine levels, thereby achieving synergistic anti-inflammatory and antioxidant effects. Collectively, these mechanisms substantially attenuate CP progression. This targeted ROS-scavenging and anti-inflammatory strategy holds promise as an alternative therapeutic approach for chronic pancreatitis.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"37 ","pages":"Article 102811"},"PeriodicalIF":10.2,"publicationDate":"2026-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146079017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Precisely targeted delivery of antitumor agents is a key strategy for enhancing cancer treatment efficacy. By leveraging specific tumor characteristics, functional modifications can construct highly selective delivery systems to optimize nanomedicine distribution at tumor sites. Owing to their editable sequences, peptides can be engineered into various targeting ligands for nanomaterial functionalization. Through specific receptor-ligand interactions, these modified nanomaterials achieve enhanced tumor-specific localization and deep penetration, enabling precise therapeutic agent delivery and improved treatment outcomes. This paper systematically reviews recent advances in peptide-based nanomaterials for tumor-targeted therapy. Based on molecular recognition, we present their applications in targeting the tumor microenvironment (TME) (including vasculature,immune cells, extracellular matrix, and associated fibroblasts), tumor cells, and organelles (such as mitochondria, endoplasmic reticulum [ER], Golgi apparatus, and nucleus). Furthermore, we provide an in-depth discussion of the opportunities and challenges these materials face in drug-targeted delivery, aiming to support the advancement of tumor-targeting nanomedicine.
{"title":"Advancing precision tumor therapy: Progress in targeted delivery of peptide-based nanomaterials from microenvironment to organelles","authors":"Kexin Tian , Jiabao Sheng , Jiao Chen , Mingjun Zhang , Jiarui Song , Manqing Wu , Yinan Zhao , Shubiao Zhang","doi":"10.1016/j.mtbio.2026.102820","DOIUrl":"10.1016/j.mtbio.2026.102820","url":null,"abstract":"<div><div>Precisely targeted delivery of antitumor agents is a key strategy for enhancing cancer treatment efficacy. By leveraging specific tumor characteristics, functional modifications can construct highly selective delivery systems to optimize nanomedicine distribution at tumor sites. Owing to their editable sequences, peptides can be engineered into various targeting ligands for nanomaterial functionalization. Through specific receptor-ligand interactions, these modified nanomaterials achieve enhanced tumor-specific localization and deep penetration, enabling precise therapeutic agent delivery and improved treatment outcomes. This paper systematically reviews recent advances in peptide-based nanomaterials for tumor-targeted therapy. Based on molecular recognition, we present their applications in targeting the tumor microenvironment (TME) (including vasculature,immune cells, extracellular matrix, and associated fibroblasts), tumor cells, and organelles (such as mitochondria, endoplasmic reticulum [ER], Golgi apparatus, and nucleus). Furthermore, we provide an in-depth discussion of the opportunities and challenges these materials face in drug-targeted delivery, aiming to support the advancement of tumor-targeting nanomedicine.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"37 ","pages":"Article 102820"},"PeriodicalIF":10.2,"publicationDate":"2026-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146079630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-21DOI: 10.1016/j.mtbio.2026.102839
Jingqiu Chen , Hegeng Li , Yanbing Tao , Wenjian Zhang , Xinyi Chen , Yunong Zhao , Lanpeng Guo , Qing Huang , Jianjun Chen , Huan Liu
Rhinitis is a common chronic respiratory disease that severely affects patients' quality of life and may lead to serious complications, underscoring the need for rapid and portable diagnostic tools to support routine monitoring and clinical management. Here, we introduce a multiscale material platform based on colloidal quantum dots (CQDs) for the construction of electrochemical immunosensors enabling the rapid detection of eosinophil cationic protein (ECP) and neutrophil myeloperoxidase (MPO), two clinically relevant biomarkers of rhinitis. The platform leverages specific antigen–antibody recognition coupled with the electrical transduction properties of CQDs, resulting in significant improvement of detection sensitivity. To ensure translational applicability, we systematically examined key factors associated with real clinical samples, including hemolysis, storage duration, and preservation conditions in serum, and verified the method's reliability through spiked recovery experiments. More over, the immunosensor was successfully applied to the detection of ECP and MPO in patient-derived nasal secretion samples, exhibiting strong concordance with enzyme-linked immunosorbent assay (ELISA) results. Collectively, this work demonstrates that the CQD-based material platform offers a robust and clinically validated strategy for protein biomarker detection, and highlights its strong potential for home-based diagnostics, providing valuable support for the early diagnosis and management of rhinitis.
{"title":"A multiscale quantum dots-based material platform for high-performance immunosensing of rhinitis biomarkers","authors":"Jingqiu Chen , Hegeng Li , Yanbing Tao , Wenjian Zhang , Xinyi Chen , Yunong Zhao , Lanpeng Guo , Qing Huang , Jianjun Chen , Huan Liu","doi":"10.1016/j.mtbio.2026.102839","DOIUrl":"10.1016/j.mtbio.2026.102839","url":null,"abstract":"<div><div>Rhinitis is a common chronic respiratory disease that severely affects patients' quality of life and may lead to serious complications, underscoring the need for rapid and portable diagnostic tools to support routine monitoring and clinical management. Here, we introduce a multiscale material platform based on colloidal quantum dots (CQDs) for the construction of electrochemical immunosensors enabling the rapid detection of eosinophil cationic protein (ECP) and neutrophil myeloperoxidase (MPO), two clinically relevant biomarkers of rhinitis. The platform leverages specific antigen–antibody recognition coupled with the electrical transduction properties of CQDs, resulting in significant improvement of detection sensitivity. To ensure translational applicability, we systematically examined key factors associated with real clinical samples, including hemolysis, storage duration, and preservation conditions in serum, and verified the method's reliability through spiked recovery experiments. More over, the immunosensor was successfully applied to the detection of ECP and MPO in patient-derived nasal secretion samples, exhibiting strong concordance with enzyme-linked immunosorbent assay (ELISA) results. Collectively, this work demonstrates that the CQD-based material platform offers a robust and clinically validated strategy for protein biomarker detection, and highlights its strong potential for home-based diagnostics, providing valuable support for the early diagnosis and management of rhinitis.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"37 ","pages":"Article 102839"},"PeriodicalIF":10.2,"publicationDate":"2026-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146079014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-21DOI: 10.1016/j.mtbio.2026.102835
Yuxiu Wang , Haoyang Yuan , Ye Zhang , Yu Zhang , Tian Yin , Haibing He , Xing Tang , Yanjiao Wang , Jingxin Gou , Guofei Li
The immunosuppressive tumor microenvironment and poor drug targeting remain major obstacles in bladder cancer (BC) therapy. To address this, a combination strategy integrating chemotherapy and immunotherapy was employed by co-delivering epirubicin (EPI) and the immune modulator imiquimod (IMQ) using a folate-modified nanocarrier. A hydroxyethyl starch-based epirubicin prodrug modified with folic acid (FA-HES-EPI) was first synthesized to improve tumor selectivity. FA-HES-EPI/IMQ micelles were then fabricated via nanoprecipitation by encapsulating IMQ into the hydrophobic core, aiming to achieve synergistic therapeutic efficacy. Folate modification conferred tumor-targeting capability to the micelles and promoted efficient cellular uptake via folate receptor–mediated endocytosis. The acid-sensitive hydrazone bond enabled controlled release of both EPI and IMQ in the acidic tumor microenvironment, thereby enhancing their combined chemo-immunotherapeutic effects. In an orthotopic BC model, FA-HES-EPI/IMQ micelles significantly enhanced drug accumulation at the tumor site, repolarized M2-type tumor-associated macrophages (TAMs) toward the M1 phenotype, remodeled the tumor stroma, and achieved a tumor inhibition rate of 96.7 %, markedly surpassing that of FA-HES-EPI micelles (86.6 %) and free EPI (62.3 %), with negligible systemic toxicity. This pH-responsive co-delivery system represents a promising approach to improve both efficacy and safety in bladder cancer treatment.
{"title":"Intravesical folate-conjugated hydroxyethyl starch micelles for pH-triggered co-delivery of epirubicin and TLR7 agonist toward synergistic chemoimmunotherapy of bladder cancer","authors":"Yuxiu Wang , Haoyang Yuan , Ye Zhang , Yu Zhang , Tian Yin , Haibing He , Xing Tang , Yanjiao Wang , Jingxin Gou , Guofei Li","doi":"10.1016/j.mtbio.2026.102835","DOIUrl":"10.1016/j.mtbio.2026.102835","url":null,"abstract":"<div><div>The immunosuppressive tumor microenvironment and poor drug targeting remain major obstacles in bladder cancer (BC) therapy. To address this, a combination strategy integrating chemotherapy and immunotherapy was employed by co-delivering epirubicin (EPI) and the immune modulator imiquimod (IMQ) using a folate-modified nanocarrier. A hydroxyethyl starch-based epirubicin prodrug modified with folic acid (FA-HES-EPI) was first synthesized to improve tumor selectivity. FA-HES-EPI/IMQ micelles were then fabricated via nanoprecipitation by encapsulating IMQ into the hydrophobic core, aiming to achieve synergistic therapeutic efficacy. Folate modification conferred tumor-targeting capability to the micelles and promoted efficient cellular uptake via folate receptor–mediated endocytosis. The acid-sensitive hydrazone bond enabled controlled release of both EPI and IMQ in the acidic tumor microenvironment, thereby enhancing their combined chemo-immunotherapeutic effects. In an orthotopic BC model, FA-HES-EPI/IMQ micelles significantly enhanced drug accumulation at the tumor site, repolarized M2-type tumor-associated macrophages (TAMs) toward the M1 phenotype, remodeled the tumor stroma, and achieved a tumor inhibition rate of 96.7 %, markedly surpassing that of FA-HES-EPI micelles (86.6 %) and free EPI (62.3 %), with negligible systemic toxicity. This pH-responsive co-delivery system represents a promising approach to improve both efficacy and safety in bladder cancer treatment.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"37 ","pages":"Article 102835"},"PeriodicalIF":10.2,"publicationDate":"2026-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146024540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-21DOI: 10.1016/j.mtbio.2026.102837
Ji Yin , Xiaojun Mao , Panpan Shang , Shuo Chen , Guang Yang , Hongtao He , Chuanglong He , Xiaojun Zhou
3D bioprinting enables the fabrication of biomimetic, cell-laden and pro-osteogenic constructs with high precision for bone regeneration. The ability of integrating favorable mechanical strength and multi-material interactive bioactivity in engineered constructs for efficient bone defect repair is still a challenge. Herein, we employed a dual-nozzle synergistic 3D bioprinting technology to fabricate a biocomposite scaffold that integrated interactive soft hydrogel filaments and hard polycaprolactone (PCL)-based filaments by mimicking weave patterns. The multi-material scaffold design aimed at providing features of suitable microstructure and long-term mechanical support, enhanced vascularized bone regeneration for bone repair. Chitosan/hyaluronic acid functionalized mesoporous silica nanoparticles bearing osteogenic protein on the surface and angiogenic drug in the pores were embedded into cell-supportive hydrogel bioink for promoting osteogenesis-angiogenesis coupling. Meanwhile, MgO nanoparticles were incorporated into structure-supportive PCL matrix for improving mechanical strength and compensating angiogenic/osteogenic activities by sustained release of Mg2+. The biocomposite scaffold had good cytocompatibility, and could stimulate in vitro angiogenic behavior and osteogenic differentiation. In vivo experiments revealed that the biocomposite scaffolds significantly enhanced vascularization and promoted bone regeneration on the cranial defect model. Overall, this study has offered a promising strategy for fabricating a multi-level adaptable 3D-bioprinted scaffold for bone defect repair through osteogenesis-angiogenesis coupling.
{"title":"Synergistic 3D-bioprinted scaffold with multi-level adaptability for vascularized bone regeneration via osteogenesis-angiogenesis coupling","authors":"Ji Yin , Xiaojun Mao , Panpan Shang , Shuo Chen , Guang Yang , Hongtao He , Chuanglong He , Xiaojun Zhou","doi":"10.1016/j.mtbio.2026.102837","DOIUrl":"10.1016/j.mtbio.2026.102837","url":null,"abstract":"<div><div>3D bioprinting enables the fabrication of biomimetic, cell-laden and pro-osteogenic constructs with high precision for bone regeneration. The ability of integrating favorable mechanical strength and multi-material interactive bioactivity in engineered constructs for efficient bone defect repair is still a challenge. Herein, we employed a dual-nozzle synergistic 3D bioprinting technology to fabricate a biocomposite scaffold that integrated interactive soft hydrogel filaments and hard polycaprolactone (PCL)-based filaments by mimicking weave patterns. The multi-material scaffold design aimed at providing features of suitable microstructure and long-term mechanical support, enhanced vascularized bone regeneration for bone repair. Chitosan/hyaluronic acid functionalized mesoporous silica nanoparticles bearing osteogenic protein on the surface and angiogenic drug in the pores were embedded into cell-supportive hydrogel bioink for promoting osteogenesis-angiogenesis coupling. Meanwhile, MgO nanoparticles were incorporated into structure-supportive PCL matrix for improving mechanical strength and compensating angiogenic/osteogenic activities by sustained release of Mg<sup>2+</sup>. The biocomposite scaffold had good cytocompatibility, and could stimulate <em>in vitro</em> angiogenic behavior and osteogenic differentiation. <em>In vivo</em> experiments revealed that the biocomposite scaffolds significantly enhanced vascularization and promoted bone regeneration on the cranial defect model. Overall, this study has offered a promising strategy for fabricating a multi-level adaptable 3D-bioprinted scaffold for bone defect repair through osteogenesis-angiogenesis coupling.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"37 ","pages":"Article 102837"},"PeriodicalIF":10.2,"publicationDate":"2026-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146079670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-20DOI: 10.1016/j.mtbio.2026.102826
Xiaolei Chen , Haolei Hu , Jie Yang , Yiwen Wang , Wei Yue , Peimei Xing , Yage Zhang , Jianwei Chen , Tao Xu , Yi Li
Microtia remains a major clinical challenge, as autologous costal cartilage transplantation—the current gold standard—suffers from donor-site morbidity and imprecise morphology, whereas synthetic implants are prone to immune rejection and structural collapse. Here, we present a biphasic composite strategy integrating microfluidics and 3D bioprinting. Organoid-like auricular spheroids generated via microfluidics exhibited a biomimetic architecture, featuring cartilage-specific collagen cores surrounded by organized chondrocytes, with sustained ECM secretion and phenotype maintenance. These bioactive spheroids were subsequently incorporated into a biomimetic bioink and patterned through extrusion-based 3D bioprinting, enabling precise anatomical shaping and functional scaffold construction. Upon implantation in immunodeficient mice, the biphasic constructs promoted rapid in situ cartilage regeneration and ECM deposition, yielding tissue with morphological and histological features closely resembling native auricular cartilage. Collectively, this study demonstrates that the integration of microfluidic spheroids with 3D bioprinting offers a balanced solution between structural fidelity and biological functionality, providing a promising pathway for auricular cartilage reconstruction.
{"title":"3D bioprinted composite scaffold incorporating microfluidics-derived chondrocyte microspheroids promotes auricular cartilage regeneration","authors":"Xiaolei Chen , Haolei Hu , Jie Yang , Yiwen Wang , Wei Yue , Peimei Xing , Yage Zhang , Jianwei Chen , Tao Xu , Yi Li","doi":"10.1016/j.mtbio.2026.102826","DOIUrl":"10.1016/j.mtbio.2026.102826","url":null,"abstract":"<div><div>Microtia remains a major clinical challenge, as autologous costal cartilage transplantation—the current gold standard—suffers from donor-site morbidity and imprecise morphology, whereas synthetic implants are prone to immune rejection and structural collapse. Here, we present a biphasic composite strategy integrating microfluidics and 3D bioprinting. Organoid-like auricular spheroids generated via microfluidics exhibited a biomimetic architecture, featuring cartilage-specific collagen cores surrounded by organized chondrocytes, with sustained ECM secretion and phenotype maintenance. These bioactive spheroids were subsequently incorporated into a biomimetic bioink and patterned through extrusion-based 3D bioprinting, enabling precise anatomical shaping and functional scaffold construction. Upon implantation in immunodeficient mice, the biphasic constructs promoted rapid in situ cartilage regeneration and ECM deposition, yielding tissue with morphological and histological features closely resembling native auricular cartilage. Collectively, this study demonstrates that the integration of microfluidic spheroids with 3D bioprinting offers a balanced solution between structural fidelity and biological functionality, providing a promising pathway for auricular cartilage reconstruction.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"37 ","pages":"Article 102826"},"PeriodicalIF":10.2,"publicationDate":"2026-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146024484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Early brain injury (EBI) has been identified as a key factor leading to the poor prognosis of patients with subarachnoid hemorrhage (SAH). At present, apart from surgical treatment, there is a lack of effective neuroprotective drugs. In this study, a biomimetic nanozyme V-MDL-800 was constructed by coordinating Vanadium Single-atom enzymes (V/SAE) and the allosteric activator MDL-800 of Sirt6, and encapsulated into NM@V-MDL-800 with neutropenia cell membrane (NM). By clearing ROS, the xCT/GPX4 pathway was activated, blocking the pathophysiological process of EBI after SAH can improve prognosis. NM@V-MDL-800 recruits through the blood-brain barrier (BBB) at the site of hemorrhagic injury by relying on the chemotactic property of neutrophils. Among them, the catalase-like, superoxide dismutase-like, and hydroxyl radical scavenging effects of V/SAE can eliminate excessive reactive oxygen species (ROS) within cells and inhibit oxidative stress; at the same time, as an allosteric activator of Sirt6, it activates the downstream xCT/GPX4 pathway, improving lipid metabolism abnormalities. Regulating the key core pathway of lipid peroxidation on ferroptosis promotes the polarization of microglia from the pro-inflammatory M1 form to the anti-inflammatory M2 morphology to inhibit the pathophysiological process of neuroinflammation in EBI. In addition, in vivo imaging of mice confirmed the targeted effect of NM@V-MDL-800 through the blood-brain barrier and recruited at the site of bleeding injury. The therapeutic effect of NM@V-MDL-800 on the SAH model has also been confirmed in vivo and in vitro experiments. This provides new ideas for SAH drug therapy regimens of SAH targeting microglial ferroptosis.
{"title":"Inflammation-targeted single-atom nanozymes drive microglial depolarization and inhibit ferroptosis via Sirt-6-xCT-GPX4 axis to attenuate early brain injury following subarachnoid hemorrhage","authors":"Boliang Liu , Chao Xiang , Xiaodan Zhang , Wei Guo , Haitao Wu , Fandi Hou , Yueyang Ba , Xiulei Zhang , Zhongcan Chen , Guang Feng , Yuan Dang , Yang Zhu , Jianjun Gu","doi":"10.1016/j.mtbio.2026.102829","DOIUrl":"10.1016/j.mtbio.2026.102829","url":null,"abstract":"<div><div>Early brain injury (EBI) has been identified as a key factor leading to the poor prognosis of patients with subarachnoid hemorrhage (SAH). At present, apart from surgical treatment, there is a lack of effective neuroprotective drugs. In this study, a biomimetic nanozyme V-MDL-800 was constructed by coordinating Vanadium Single-atom enzymes (V/SAE) and the allosteric activator MDL-800 of Sirt6, and encapsulated into NM@V-MDL-800 with neutropenia cell membrane (NM). By clearing ROS, the xCT/GPX4 pathway was activated, blocking the pathophysiological process of EBI after SAH can improve prognosis. NM@V-MDL-800 recruits through the blood-brain barrier (BBB) at the site of hemorrhagic injury by relying on the chemotactic property of neutrophils. Among them, the catalase-like, superoxide dismutase-like, and hydroxyl radical scavenging effects of V/SAE can eliminate excessive reactive oxygen species (ROS) within cells and inhibit oxidative stress; at the same time, as an allosteric activator of Sirt6, it activates the downstream xCT/GPX4 pathway, improving lipid metabolism abnormalities. Regulating the key core pathway of lipid peroxidation on ferroptosis promotes the polarization of microglia from the pro-inflammatory M1 form to the anti-inflammatory M2 morphology to inhibit the pathophysiological process of neuroinflammation in EBI. In addition, in vivo imaging of mice confirmed the targeted effect of NM@V-MDL-800 through the blood-brain barrier and recruited at the site of bleeding injury. The therapeutic effect of NM@V-MDL-800 on the SAH model has also been confirmed in vivo and in vitro experiments. This provides new ideas for SAH drug therapy regimens of SAH targeting microglial ferroptosis.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"37 ","pages":"Article 102829"},"PeriodicalIF":10.2,"publicationDate":"2026-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146024482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-19DOI: 10.1016/j.mtbio.2026.102800
Chalom Zemmour , Mor Ozeri , Ora T. Cohen , Eduard Berenshtein , Zakhariya Manevitch , Yael Feinstein-Rotkopf , Irit Rosenhek-Goldian , Hadar Benyamini , Victor Shelukhin , Ofra Benny
Accurate and rapid identification of aggressive cancer cells remains a major clinical challenge. Here, we present a simple, label-free mechanophenotyping platform that integrates controlled colloidal topographies with particle-uptake measurements to reveal biophysical traits associated with metastatic progression. Non-close-packed polystyrene bead arrays were formed on cell culture plates by controlled deposition and stabilized with a thin silicon oxide coating. These arrays display micro- and nano-features with a size range of 0.23–2.3 μm at diverse densities and were used to assess adhesion across cancer cells exhibiting different levels of malignancy. Particle uptake differences were most pronounced for particle diameters above 0.5 μm, whereas adhesion differences emerged predominantly on particles ≥0.7 μm and increased progressively with larger particle sizes. Colloidal topographies were fabricated at particle deposition concentrations of 500 μg/mL and 1000 μg/mL, and adhesion differences were observed under both conditions, with more potent effects at the higher concentration. At the metastatic site, cells exhibited increased particle uptake, stronger adhesion, and a larger morphological engagement on colloid-coated substrates, characterized by extensive actin-rich protrusions wrapping individual particles. AFM force mapping confirmed higher adhesion forces to a colloidal probe, while transcriptomic profiling revealed enrichment of adhesion and ECM-remodeling pathways in the adhesive metastatic state. We also find that lymphatically selected cells exhibit reduced adhesion on colloid-coated surfaces but higher particle uptake compared to the primary tumor cells. These results indicate that after leaving the primary tumor, metastatic cells have reduced adhesive potential, which is only regained upon reaching secondary sites. By exposing adhesion differences that are undetectable on flat substrates and linking them to particle uptake assays, this platform produces functional signatures of metastatic potential. This method is technically accessible, compatible with imaging and molecular workflows, and adaptable for high-throughput or clinical analysis, offering a potential route for label-free detection and classification of cancer cells by their aggressiveness.
{"title":"Colloid-patterned surfaces distinguish malignant mechanophenotypes","authors":"Chalom Zemmour , Mor Ozeri , Ora T. Cohen , Eduard Berenshtein , Zakhariya Manevitch , Yael Feinstein-Rotkopf , Irit Rosenhek-Goldian , Hadar Benyamini , Victor Shelukhin , Ofra Benny","doi":"10.1016/j.mtbio.2026.102800","DOIUrl":"10.1016/j.mtbio.2026.102800","url":null,"abstract":"<div><div>Accurate and rapid identification of aggressive cancer cells remains a major clinical challenge. Here, we present a simple, label-free mechanophenotyping platform that integrates controlled colloidal topographies with particle-uptake measurements to reveal biophysical traits associated with metastatic progression. Non-close-packed polystyrene bead arrays were formed on cell culture plates by controlled deposition and stabilized with a thin silicon oxide coating. These arrays display micro- and nano-features with a size range of 0.23–2.3 μm at diverse densities and were used to assess adhesion across cancer cells exhibiting different levels of malignancy. Particle uptake differences were most pronounced for particle diameters above 0.5 μm, whereas adhesion differences emerged predominantly on particles ≥0.7 μm and increased progressively with larger particle sizes. Colloidal topographies were fabricated at particle deposition concentrations of 500 μg/mL and 1000 μg/mL, and adhesion differences were observed under both conditions, with more potent effects at the higher concentration. At the metastatic site, cells exhibited increased particle uptake, stronger adhesion, and a larger morphological engagement on colloid-coated substrates, characterized by extensive actin-rich protrusions wrapping individual particles. AFM force mapping confirmed higher adhesion forces to a colloidal probe, while transcriptomic profiling revealed enrichment of adhesion and ECM-remodeling pathways in the adhesive metastatic state. We also find that lymphatically selected cells exhibit reduced adhesion on colloid-coated surfaces but higher particle uptake compared to the primary tumor cells. These results indicate that after leaving the primary tumor, metastatic cells have reduced adhesive potential, which is only regained upon reaching secondary sites. By exposing adhesion differences that are undetectable on flat substrates and linking them to particle uptake assays, this platform produces functional signatures of metastatic potential. This method is technically accessible, compatible with imaging and molecular workflows, and adaptable for high-throughput or clinical analysis, offering a potential route for label-free detection and classification of cancer cells by their aggressiveness.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"37 ","pages":"Article 102800"},"PeriodicalIF":10.2,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146024396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-19DOI: 10.1016/j.mtbio.2026.102799
Annabelle Neuhäusler , Nils Lindner , Andreas Blaeser
Hydrogels emerged as versatile biomaterials for tissue engineering due to their extra cellular matrix similarity and mechanical and biochemical properties. Still, hydrogels expose limited stiffness, anisotropy and nutrient diffusion. By reinforcing hydrogels with synthetic and natural fibers, these drawbacks can be effectively addressed, thereby enabling the modeling of advanced biomimetic tissue. This review discusses recent progress in the fabrication of fiber-integrated hydrogels and brings together developments from biomaterials, biofabrication, mechanobiology, and organ-model engineering. Fiber-addition impact on viscoelastic, time-dependent und nonlinear material properties, on multiscale and hierarchical constructs and on mechanical and biological readouts are analyzed. Specifically, the integration of both synthetic and natural fibers into hydrogel matrices is highlighted which significantly broaden their structural and biochemical versatility. These fiber-added hydrogels display improved properties including enhanced stiffness (up to 10-fold increase), anisotropy (>80 % alignment) and nutrient diffusion (4-fold increase). Moreover, the incorporation of fibers directly impacts cellular behavior by promoting adhesion, migration, proliferation and differentiation. Finally, bone, muscle and nerve tissue are exemplary presented in more detail to highlight the broad potential of these composite materials. In conclusion, fiber-embedded hydrogels represent a decisive step toward enhanced 4D-metamaterials.
{"title":"Fiber-integrated hydrogels: a versatile platform to improve structural and biological performance in 3D biofabrication","authors":"Annabelle Neuhäusler , Nils Lindner , Andreas Blaeser","doi":"10.1016/j.mtbio.2026.102799","DOIUrl":"10.1016/j.mtbio.2026.102799","url":null,"abstract":"<div><div>Hydrogels emerged as versatile biomaterials for tissue engineering due to their extra cellular matrix similarity and mechanical and biochemical properties. Still, hydrogels expose limited stiffness, anisotropy and nutrient diffusion. By reinforcing hydrogels with synthetic and natural fibers, these drawbacks can be effectively addressed, thereby enabling the modeling of advanced biomimetic tissue. This review discusses recent progress in the fabrication of fiber-integrated hydrogels and brings together developments from biomaterials, biofabrication, mechanobiology, and organ-model engineering. Fiber-addition impact on viscoelastic, time-dependent und nonlinear material properties, on multiscale and hierarchical constructs and on mechanical and biological readouts are analyzed. Specifically, the integration of both synthetic and natural fibers into hydrogel matrices is highlighted which significantly broaden their structural and biochemical versatility. These fiber-added hydrogels display improved properties including enhanced stiffness (up to 10-fold increase), anisotropy (>80 % alignment) and nutrient diffusion (4-fold increase). Moreover, the incorporation of fibers directly impacts cellular behavior by promoting adhesion, migration, proliferation and differentiation. Finally, bone, muscle and nerve tissue are exemplary presented in more detail to highlight the broad potential of these composite materials. In conclusion, fiber-embedded hydrogels represent a decisive step toward enhanced 4D-metamaterials.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"37 ","pages":"Article 102799"},"PeriodicalIF":10.2,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146024535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-19DOI: 10.1016/j.mtbio.2026.102823
Liwen Chen , Chao Shang , Yang Hong , Hui Zhang
Glioblastoma (GBM) is a highly aggressive brain tumor that offers few treatment options. Current therapeutic approaches face challenges such as poor drug delivery precision, low accumulation at tumor sites, and significant toxicity to normal tissues. To address these issues, this study designed a targeted drug delivery platform by modifying liposomes with the tumor-homing peptide A2b11, which specifically binds to the GBM-associated antigen IL13RA2. A2b11-liposomes loaded with sodium fluorescein (FLA) and A2b11-liposomes loaded with temozolomide (TLA) were evaluated for their anti-GBM effects. In vitro, studies demonstrated that A2b11-modified liposomes significantly enhanced accumulation in GBM cells and enhanced their anti-GBM efficacy compared to unmodified liposomes. In vivo, experiments using xenograft tumor mouse models showed that the targeted drug delivery platform exhibited good biocompatibility and in vivo safety. Compared to other organs, FLA demonstrated significantly higher accumulation at tumor sites, showcasing its potential for targeted therapy and enabling assessment of its effects on inducing apoptosis and inhibiting angiogenesis. Additionally, TLA induced GBM cell apoptosis and reduced tumor angiogenesis, highlighting its significant potential in inhibiting GBM tumor growth. The A2b11 peptide-modified liposomal system developed in this study represents a promising platform for GBM-targeted therapy. Further research and development of this platform could lay the groundwork for its future clinical application.
{"title":"Development and evaluation of IL13RA2 targeted drug delivery system based on glioblastoma homing peptide A2b11","authors":"Liwen Chen , Chao Shang , Yang Hong , Hui Zhang","doi":"10.1016/j.mtbio.2026.102823","DOIUrl":"10.1016/j.mtbio.2026.102823","url":null,"abstract":"<div><div>Glioblastoma (GBM) is a highly aggressive brain tumor that offers few treatment options. Current therapeutic approaches face challenges such as poor drug delivery precision, low accumulation at tumor sites, and significant toxicity to normal tissues. To address these issues, this study designed a targeted drug delivery platform by modifying liposomes with the tumor-homing peptide A2b11, which specifically binds to the GBM-associated antigen IL13RA2. A2b11-liposomes loaded with sodium fluorescein (FLA) and A2b11-liposomes loaded with temozolomide (TLA) were evaluated for their anti-GBM effects. In vitro, studies demonstrated that A2b11-modified liposomes significantly enhanced accumulation in GBM cells and enhanced their anti-GBM efficacy compared to unmodified liposomes. In vivo, experiments using xenograft tumor mouse models showed that the targeted drug delivery platform exhibited good biocompatibility and in vivo safety. Compared to other organs, FLA demonstrated significantly higher accumulation at tumor sites, showcasing its potential for targeted therapy and enabling assessment of its effects on inducing apoptosis and inhibiting angiogenesis. Additionally, TLA induced GBM cell apoptosis and reduced tumor angiogenesis, highlighting its significant potential in inhibiting GBM tumor growth. The A2b11 peptide-modified liposomal system developed in this study represents a promising platform for GBM-targeted therapy. Further research and development of this platform could lay the groundwork for its future clinical application.</div></div>","PeriodicalId":18310,"journal":{"name":"Materials Today Bio","volume":"37 ","pages":"Article 102823"},"PeriodicalIF":10.2,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146024536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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