Accurate navigation of intravascular interventional catheters and precise thrombus localization are critical for effective cerebrovascular procedures. Traditional digital subtraction angiography (DSA) offers navigation and visualization but is limited by two-dimensional imaging, increased procedural time, elevated costs, and radiation exposure. This study introduces the Hybrid Electrodes Electric Field Stereotaxis (HEEFS) method, which employs an internal localization electrode and external measurement electrodes to generate an electric field. The movement of the localization electrode induces changes in the field, enabling real-time 3D catheter navigation and thrombus localization based on the field’s uniqueness theorem. Unlike DSA, HEEFS achieves three-dimensional localization without additional imaging equipment and detects guidewire-thrombus contact via power monitoring. Simulation and porcine blood experiments demonstrated a localization accuracy of less than 1.7 mm and effective assessment of thrombus removal completeness. These results highlight the potential of HEEFS as a radiation-free, real-time navigation method, providing a novel solution for intravascular interventional surgeries.