Background : Diabetes is a metabolic disorder with chemical, physiological and pathological changes in the liver. Due to the side effects of insulin, the use of herbal medicines such as Nigella sativa with flavonoid compounds and antioxidant properties is considered. In this study, the effect of magnetic hydroethanolic extract of Nigella sativa and MgO nanoparticles separately and simultaneously on the expression of CAT gene in liver tissue of male diabetic rats was investigated. Materials and methods : 40 male Wistar rats, after induction of diabetes by injection of streptozotomycin (STZ) at a dose of 60 mg / kg, were treated by magnetic hydroathanolic extract of Nigella sativa at a dose of 250 mg / kg, MgO nanoparticles at a dose of 10 mg / kg, combined magnetic hydroathanolic extract 125 mg / kg and 5 mg / kg MgO nanoparticles, and the extract loaded with MgO nanoparticles every other day for 28 days and the expression of liver CAT gene was evaluated. Results : The extract loaded with MgO nanoparticles was able to increase CAT gene expression in diabetics to the extent of gene expression in metformin-treated diabetics. Conclusion : It seems that thymoquinone (TQ) in Nigella sativa due to its antioxidant properties increases the expression of CAT gene and overcomes the oxidative stress caused by diabetes with its protective effect on pancreatic beta cells. Loading the nanoparticles probably resulted in better absorption of the antioxidant compounds in the cell, and the MgO nanoparticles as nanocontractors facilitated the cell's access to the particulate compounds.
{"title":"Effects of magnetic hydroethanolic extract of Nigella sativa and magnesium oxide nanoparticles (MgO) on catalase gene expression in liver of diabetic male rats","authors":"Mahboubeh Amini, Maryam Ostadsharif, N. Naghsh","doi":"10.52547/iau.32.2.123","DOIUrl":"https://doi.org/10.52547/iau.32.2.123","url":null,"abstract":"Background : Diabetes is a metabolic disorder with chemical, physiological and pathological changes in the liver. Due to the side effects of insulin, the use of herbal medicines such as Nigella sativa with flavonoid compounds and antioxidant properties is considered. In this study, the effect of magnetic hydroethanolic extract of Nigella sativa and MgO nanoparticles separately and simultaneously on the expression of CAT gene in liver tissue of male diabetic rats was investigated. Materials and methods : 40 male Wistar rats, after induction of diabetes by injection of streptozotomycin (STZ) at a dose of 60 mg / kg, were treated by magnetic hydroathanolic extract of Nigella sativa at a dose of 250 mg / kg, MgO nanoparticles at a dose of 10 mg / kg, combined magnetic hydroathanolic extract 125 mg / kg and 5 mg / kg MgO nanoparticles, and the extract loaded with MgO nanoparticles every other day for 28 days and the expression of liver CAT gene was evaluated. Results : The extract loaded with MgO nanoparticles was able to increase CAT gene expression in diabetics to the extent of gene expression in metformin-treated diabetics. Conclusion : It seems that thymoquinone (TQ) in Nigella sativa due to its antioxidant properties increases the expression of CAT gene and overcomes the oxidative stress caused by diabetes with its protective effect on pancreatic beta cells. Loading the nanoparticles probably resulted in better absorption of the antioxidant compounds in the cell, and the MgO nanoparticles as nanocontractors facilitated the cell's access to the particulate compounds.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85912001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Somayeh Abolghasemi-Dehaghani, M. Gharanfoli, M. Habibi-Rezaei, Ramezan-Ali Khavari-Nejad
The production of biotechnological drug proteins plays an important role against disease. The process of producing drug recombinant proteins is not a direct path, because it requires a lot of work and on the other hand, one of the important and significant aspects in the production of proteins is the discussion of their stability and solubility during the expression and purification process. Proteins are stable only in a limited range of temperature and acidity conditions and are highly susceptible to physical and chemical degradation. Today, various solutions have been proposed to increase the solubility and stability of proteins, including guided changes in the protein molecule, and optimization of the instructions for expression, purification and solubility of proteins, which is often the first approach that is not possible. This review article provides solutions to increase the stability of proteins at different levels, including the study of the effect of expression construct design, protein tags and sequences on stability, optimal host cell selection and improved expression conditions. The effect of optimizing buffers used in pharmaceutical formulations such as selecting appropriate amino acids and osmolytes to increase protein stability and how sugars and polyols affect the stability of recombinant pharmaceutical proteins is also investigated.
{"title":"A review of methods to increase the stability of recombinant pharmaceutical proteins during the production and storage process","authors":"Somayeh Abolghasemi-Dehaghani, M. Gharanfoli, M. Habibi-Rezaei, Ramezan-Ali Khavari-Nejad","doi":"10.52547/iau.32.2.111","DOIUrl":"https://doi.org/10.52547/iau.32.2.111","url":null,"abstract":"The production of biotechnological drug proteins plays an important role against disease. The process of producing drug recombinant proteins is not a direct path, because it requires a lot of work and on the other hand, one of the important and significant aspects in the production of proteins is the discussion of their stability and solubility during the expression and purification process. Proteins are stable only in a limited range of temperature and acidity conditions and are highly susceptible to physical and chemical degradation. Today, various solutions have been proposed to increase the solubility and stability of proteins, including guided changes in the protein molecule, and optimization of the instructions for expression, purification and solubility of proteins, which is often the first approach that is not possible. This review article provides solutions to increase the stability of proteins at different levels, including the study of the effect of expression construct design, protein tags and sequences on stability, optimal host cell selection and improved expression conditions. The effect of optimizing buffers used in pharmaceutical formulations such as selecting appropriate amino acids and osmolytes to increase protein stability and how sugars and polyols affect the stability of recombinant pharmaceutical proteins is also investigated.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74136501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Akbar Malekahmadi, M. Roozbahani, Hamidreza Hekmat, Masood Sadeghi
Background : Mild traumatic brain injury (MTBI) is the least brain injury due to direct or indirect blow to the head, which according to the Glasgow scale is given a score between 13 and 15. The findings indicate cognitive impairment. Perceptual dysfunction is one of the most important cognitive deficits in these patients. The present study was designed and performed to compare MTBI patients with healthy individuals in terms of visual perception error (VPE). Materials and methods : In this causal-comparative study, 30 MTBI patients, in the range of 15-45 and mean age of 27 years and 10 months, with a diagnostic history of less than one year in Ilam hospitals, and 30 healthy individuals in the same range and mean age of 29 years and 7 months (both groups with equal sex) were randomly selected and assessed by VPEMT. Data were analyzed by independent t-test and Levin using SPSS 23. Results : The results showed that the mean VPE in patients with MTBI with unequal variances (P <0.05), at the level of 95% is significantly higher than healthy individuals (P <0.05). Conclusion : This finding, consistent with the results of previous research, indicates the chronic cognitive consequences of concussion, even at a mild level, at least up to one year after injury, and causes a sharp increase in VPE. It is necessary to pay attention to visual perception errors in clinical and legal evaluations of these patients. However, a definitive judgment in this regard requires future research.
{"title":"Comparison of visual perception error among patients with mild traumatic brain injury with healthy people","authors":"Akbar Malekahmadi, M. Roozbahani, Hamidreza Hekmat, Masood Sadeghi","doi":"10.52547/iau.32.2.196","DOIUrl":"https://doi.org/10.52547/iau.32.2.196","url":null,"abstract":"Background : Mild traumatic brain injury (MTBI) is the least brain injury due to direct or indirect blow to the head, which according to the Glasgow scale is given a score between 13 and 15. The findings indicate cognitive impairment. Perceptual dysfunction is one of the most important cognitive deficits in these patients. The present study was designed and performed to compare MTBI patients with healthy individuals in terms of visual perception error (VPE). Materials and methods : In this causal-comparative study, 30 MTBI patients, in the range of 15-45 and mean age of 27 years and 10 months, with a diagnostic history of less than one year in Ilam hospitals, and 30 healthy individuals in the same range and mean age of 29 years and 7 months (both groups with equal sex) were randomly selected and assessed by VPEMT. Data were analyzed by independent t-test and Levin using SPSS 23. Results : The results showed that the mean VPE in patients with MTBI with unequal variances (P <0.05), at the level of 95% is significantly higher than healthy individuals (P <0.05). Conclusion : This finding, consistent with the results of previous research, indicates the chronic cognitive consequences of concussion, even at a mild level, at least up to one year after injury, and causes a sharp increase in VPE. It is necessary to pay attention to visual perception errors in clinical and legal evaluations of these patients. However, a definitive judgment in this regard requires future research.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"127 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75841756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background : The purpose of this study was to evaluate the effectiveness of education model based on problem solving approach on educational engagement of medical students of Islamic Azad University, Tehran Medical Sciences Branch. Materials and methods : This research is quasi-experimental research in terms of applied purpose. The statistical population of this study was all 5928 medical students of Islamic Azad University, Tehran Branch of Medical Sciences, of which 60 were divided into experimental and control groups. The intervention group received training based on a problem-solving approach for 8 sessions. Data were collected using Zerang Academic Involvement Questionnaire (2012) and analyzed using SPSS version 25 by MANCOVA statistical test. Results : In the pre-test stage, the mean of academic involvement was not significantly different between the two groups. Comparison of academic involvement in the intervention group showed that a significant difference was observed in the mean of this variable and its dimensions after the intervention (p= 0.05). It shows that the model of education based on the problem-solving approach has a significant effect on the academic engagement of medical students of Islamic Azad University, Tehran Branch of Medical Sciences. Conclusion : Education based on problem solving approach is one of the important factors that plays a role in students' academic engagement.
{"title":"The effectiveness of education model based on problem solving approach on educational engagement of medical students of Islamic Azad University, Tehran Medical Sciences Branch","authors":"Zahra Zafari, G. Ahghar, M. Sahebalzamani","doi":"10.52547/iau.32.2.166","DOIUrl":"https://doi.org/10.52547/iau.32.2.166","url":null,"abstract":"Background : The purpose of this study was to evaluate the effectiveness of education model based on problem solving approach on educational engagement of medical students of Islamic Azad University, Tehran Medical Sciences Branch. Materials and methods : This research is quasi-experimental research in terms of applied purpose. The statistical population of this study was all 5928 medical students of Islamic Azad University, Tehran Branch of Medical Sciences, of which 60 were divided into experimental and control groups. The intervention group received training based on a problem-solving approach for 8 sessions. Data were collected using Zerang Academic Involvement Questionnaire (2012) and analyzed using SPSS version 25 by MANCOVA statistical test. Results : In the pre-test stage, the mean of academic involvement was not significantly different between the two groups. Comparison of academic involvement in the intervention group showed that a significant difference was observed in the mean of this variable and its dimensions after the intervention (p= 0.05). It shows that the model of education based on the problem-solving approach has a significant effect on the academic engagement of medical students of Islamic Azad University, Tehran Branch of Medical Sciences. Conclusion : Education based on problem solving approach is one of the important factors that plays a role in students' academic engagement.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74860627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background : Non(cid:0)alcoholic fatty liver disease (NAFLD) is associated with an increased risk of cardiovascular disease. The aim of the present study was to examine the effect high intensity interval training (HIIT) with resveratrol supplementation on Cardiomyocytes expression of MFn1, MFn2 and OPA1 in NAFLD rats. Materials and methods : In this experimental study, forty-eight Wistar male rats were classified into two groups NAFLD (CN, n=40) and control-normal (n=8). NAFLD was induced in rats with high fat diet and then subdivided into five subgroups NAFLD, SHAM, TRNAF, SUPNAF and TRSUPNAF. Training groups have performed a running program on a motor-driven treadmill for eight weeks. Resveratrol (20 mg/kg) was injected into the SUPNAF and TRSUPNAF groups. Forty-eight hours after the last training session, rats were anesthetized; then hearts were excised and immediately frozen in liquid nitrogen and stored at -80°C for measuring the indexes. Statistical analysis was performed using a one-way analysis of variance, and significance was accepted at p ≤ 0.05. Results : Induction of fatty liver reduced expression of MFn1 (p=0.009), MFn2 (p=0.003) and OPA1 (p=0.002). Also, in TRNAF, SUPNAF and RSUPNAF groups, MFn1 (p=0.010, p=0.048 and p=0.004 respectively), MFn2 (p=0.028, p=0.007 and p=0.011 respectively) and OPA1 (p=0.019, p=0.003 and p=0.006 respectively) increased significantly compared to the NAFLD group. Conclusion : It seems that exercise training and resveratrol protects cardiomyocytes mitochondria against fatty liver disease, by reactivation fusion.
{"title":"MFn1, MFn2 and OPA1 gene expression of cardiac tissue after high intensity interval training and resveratrol injection in NAFLD rats","authors":"Roeleh Shahini, Ahmad Abdi","doi":"10.52547/iau.32.2.149","DOIUrl":"https://doi.org/10.52547/iau.32.2.149","url":null,"abstract":"Background : Non(cid:0)alcoholic fatty liver disease (NAFLD) is associated with an increased risk of cardiovascular disease. The aim of the present study was to examine the effect high intensity interval training (HIIT) with resveratrol supplementation on Cardiomyocytes expression of MFn1, MFn2 and OPA1 in NAFLD rats. Materials and methods : In this experimental study, forty-eight Wistar male rats were classified into two groups NAFLD (CN, n=40) and control-normal (n=8). NAFLD was induced in rats with high fat diet and then subdivided into five subgroups NAFLD, SHAM, TRNAF, SUPNAF and TRSUPNAF. Training groups have performed a running program on a motor-driven treadmill for eight weeks. Resveratrol (20 mg/kg) was injected into the SUPNAF and TRSUPNAF groups. Forty-eight hours after the last training session, rats were anesthetized; then hearts were excised and immediately frozen in liquid nitrogen and stored at -80°C for measuring the indexes. Statistical analysis was performed using a one-way analysis of variance, and significance was accepted at p ≤ 0.05. Results : Induction of fatty liver reduced expression of MFn1 (p=0.009), MFn2 (p=0.003) and OPA1 (p=0.002). Also, in TRNAF, SUPNAF and RSUPNAF groups, MFn1 (p=0.010, p=0.048 and p=0.004 respectively), MFn2 (p=0.028, p=0.007 and p=0.011 respectively) and OPA1 (p=0.019, p=0.003 and p=0.006 respectively) increased significantly compared to the NAFLD group. Conclusion : It seems that exercise training and resveratrol protects cardiomyocytes mitochondria against fatty liver disease, by reactivation fusion.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89193421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background : Vaginal candidiasis is a common cause of vaginal infections. Currently, for treatment of these infections, fluconazole is prescribed more than other drugs; so determining the drug resistance of Candida to fluconazole and comparing it with itraconazole as a substitute has been one of the main objectives of this study. Materials and methods : Of 180 patients with symptoms of vaginal candidiasis, clinical examination, sampling and culture were performed. Candida albicans were identified by morphological and physiological methods and non- Candida albicans candida isolates by PCR-RFLP method. Susceptibility testing for antifungal drugs was performed by disk diffusion method based on CLSI M44-A protocol. Results : Out of 180 patients, 93 patients with positive culture and 98 strain of Candida were isolated. The mean age of patients was 30±0.73 years and the most common clinical complaints was itching (>98%) and vaginal discharge (88%). Candida albicans (80.61%) was the most common causative agent. The resistance of Candida albicans to fluconazole and itraconazole was 45.6% and 16.5%, respectively, and for non-albicans candida isolates were 53% and 42%, respectively. This difference was statistically significant (P<0.0001). Conclusion : The results of this study suggest that culturing of vaginal samples have essential role for diagnosis of vaginal candidiasis, and treatment with antifungal drugs should be performed after antifungal susceptibility tests.
{"title":"Comparison of sensitivity to fluconazole and itraconazole in patients with vaginal candidiasis referred to hospitals of Islamic Azad University, Tehran Medical Sciences","authors":"Roya Torkamani, Parvaneh AdimiNaghan, M. Rahimi","doi":"10.52547/iau.32.2.157","DOIUrl":"https://doi.org/10.52547/iau.32.2.157","url":null,"abstract":"Background : Vaginal candidiasis is a common cause of vaginal infections. Currently, for treatment of these infections, fluconazole is prescribed more than other drugs; so determining the drug resistance of Candida to fluconazole and comparing it with itraconazole as a substitute has been one of the main objectives of this study. Materials and methods : Of 180 patients with symptoms of vaginal candidiasis, clinical examination, sampling and culture were performed. Candida albicans were identified by morphological and physiological methods and non- Candida albicans candida isolates by PCR-RFLP method. Susceptibility testing for antifungal drugs was performed by disk diffusion method based on CLSI M44-A protocol. Results : Out of 180 patients, 93 patients with positive culture and 98 strain of Candida were isolated. The mean age of patients was 30±0.73 years and the most common clinical complaints was itching (>98%) and vaginal discharge (88%). Candida albicans (80.61%) was the most common causative agent. The resistance of Candida albicans to fluconazole and itraconazole was 45.6% and 16.5%, respectively, and for non-albicans candida isolates were 53% and 42%, respectively. This difference was statistically significant (P<0.0001). Conclusion : The results of this study suggest that culturing of vaginal samples have essential role for diagnosis of vaginal candidiasis, and treatment with antifungal drugs should be performed after antifungal susceptibility tests.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91532451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Zangeneh, Termeh Tarjoman, Arian Shahidi, Mahnaz Valizadeh, Zahra Hanifezadeh, O. Ameli, N. Saljoughi, Behnam Farhoodi, M. Mesgarian
Background: Para-clinical abnormalities are considered as predictors of COVID-19 severity. We aimed at evaluate the relationship between laboratory and chest computed tomography (CT) scan findings and severity of COVID-19 cases. Materials and methods: We performed a retrospective study on confirmed COVID-19 patients in Amir-Al-Momenin hospital, Tehran, Iran, from February 20, 2020 to April 19, 2020. Para-clinical characteristics of the patients including chest CT scan and laboratory findings were recruited from patients' medical records. Then we evaluated the relationship between laboratory and chest CT scan findings and severity of COVID-19 cases. We performed statistical analysis using descriptive methods and analytical tests by SPSS statistical software version-24.
{"title":"The relationship between laboratory and chest computed tomography scan findings and severity of COVID-19 cases: a single center study","authors":"M. Zangeneh, Termeh Tarjoman, Arian Shahidi, Mahnaz Valizadeh, Zahra Hanifezadeh, O. Ameli, N. Saljoughi, Behnam Farhoodi, M. Mesgarian","doi":"10.52547/iau.32.2.185","DOIUrl":"https://doi.org/10.52547/iau.32.2.185","url":null,"abstract":"Background: Para-clinical abnormalities are considered as predictors of COVID-19 severity. We aimed at evaluate the relationship between laboratory and chest computed tomography (CT) scan findings and severity of COVID-19 cases. Materials and methods: We performed a retrospective study on confirmed COVID-19 patients in Amir-Al-Momenin hospital, Tehran, Iran, from February 20, 2020 to April 19, 2020. Para-clinical characteristics of the patients including chest CT scan and laboratory findings were recruited from patients' medical records. Then we evaluated the relationship between laboratory and chest CT scan findings and severity of COVID-19 cases. We performed statistical analysis using descriptive methods and analytical tests by SPSS statistical software version-24.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"85 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72680953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shabnam Movassaghi, Z. Sharifi, Seyed Mohammad Hossein Noori Mugahi
Background : Nitric Oxide (NO) is a very important signaling molecule which acts as a regulator of many physiological processes in many tissues including epithelial cell of gastrointestinal tract. In this study we investigated the effects of L-Arginine as a NO progenitor and L-NAME as a NO inhibitor on epithelial cell number and height of Jejunum in female rats. Materials and methods : 40 female rats were divided into 5 groups, containing 8 rats in each group. Except the control group, the other groups received normal saline (2 ml/kg), L-Arginine (200mg/kg), L-NAME (20mg/kg) and a mixture of two substances for L-Arginine & L-NAME group intraperitoneally for 3 days. 2 weeks later after anesthesia with ether, jejunum was expelled out and after tissue processing and staining with H&E method, the changes were assessed via light microscopy. Cell number and height were evaluated using Image Tools3 Microsoft. Statistical analysis was made by One-Way ANOVA followed by Tukey post hoc test to evaluate the statistical significance between different groups. A value of p< 0.05 was considered statistically significant. Results : There was a significant increase in the cell number and height of jejunal epithelium in L-Arginine group (P<0.05). Whereas no significant difference was observed between L-NAME, L-Arginine +L-NAME and control, Normal Saline groups. Conclusion : L- Arginine can result in proliferation of Jejunum epithelial cells whereas L-NAME has no effect on these cells.
{"title":"Morphometric study of rat jejunum enterocyte after L-Arginine and L-NAME prescription","authors":"Shabnam Movassaghi, Z. Sharifi, Seyed Mohammad Hossein Noori Mugahi","doi":"10.52547/iau.32.2.132","DOIUrl":"https://doi.org/10.52547/iau.32.2.132","url":null,"abstract":"Background : Nitric Oxide (NO) is a very important signaling molecule which acts as a regulator of many physiological processes in many tissues including epithelial cell of gastrointestinal tract. In this study we investigated the effects of L-Arginine as a NO progenitor and L-NAME as a NO inhibitor on epithelial cell number and height of Jejunum in female rats. Materials and methods : 40 female rats were divided into 5 groups, containing 8 rats in each group. Except the control group, the other groups received normal saline (2 ml/kg), L-Arginine (200mg/kg), L-NAME (20mg/kg) and a mixture of two substances for L-Arginine & L-NAME group intraperitoneally for 3 days. 2 weeks later after anesthesia with ether, jejunum was expelled out and after tissue processing and staining with H&E method, the changes were assessed via light microscopy. Cell number and height were evaluated using Image Tools3 Microsoft. Statistical analysis was made by One-Way ANOVA followed by Tukey post hoc test to evaluate the statistical significance between different groups. A value of p< 0.05 was considered statistically significant. Results : There was a significant increase in the cell number and height of jejunal epithelium in L-Arginine group (P<0.05). Whereas no significant difference was observed between L-NAME, L-Arginine +L-NAME and control, Normal Saline groups. Conclusion : L- Arginine can result in proliferation of Jejunum epithelial cells whereas L-NAME has no effect on these cells.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"2010 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81207999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Z. Jafari, Shadi Abdollah Zargar, Zahra Abdolreza Gharebagh
Background: Children on dialysis need long-term care. The child's illness and the conditions associated with his/her treatment and care expose the mother to various psychological stresses. Resilience maintains good decision-making power and performance in stressful situations. The aim of this study was to investigate of resilience status among mothers of children on dialysis. Materials and methods: This descriptive cross-sectional study was performed on all mothers of children undergoing dialysis in Children's Medical Center, Mofid Hospital, Ali Asghar Hospital and Bahrami Hospital. A total of 166 eligible mothers were selected by census and completed a two-part questionnaire including demographic information and Connor-Davidson Resilience Scale. Data analysis was performed using SPSS software version 22 at a significance level of 0.05. Results: The mean of resilience was 3.35 out of 4 points and was at strong level. Also, the mean of dimensions of personal competence/strength, trust in personal instincts, tolerance of negative emotions, control and spirituality in individuals were 3.37, 3.17, 3.43, 3.45 and 3.33, respectively. The resilience of mothers of children undergoing hemodialysis was significantly higher than children under peritoneal dialysis (p=0.047). There was no significant association between maternal age, maternal education, maternal status of morbidity, child age, child gender, number of children in the family and duration of dialysis and mean maternal resilience. Conclusion: The resilience situation was at a good level. Due to the importance of resilience, it is recommended to provide educational and empowerment interventions for parents of children with chronic diseases.
{"title":"Investigation of resilience status among mothers of children undergoing dialysis referred to selected hospitals of medical sciences universities of Tehran","authors":"Z. Jafari, Shadi Abdollah Zargar, Zahra Abdolreza Gharebagh","doi":"10.52547/iau.32.1.83","DOIUrl":"https://doi.org/10.52547/iau.32.1.83","url":null,"abstract":"Background: Children on dialysis need long-term care. The child's illness and the conditions associated with his/her treatment and care expose the mother to various psychological stresses. Resilience maintains good decision-making power and performance in stressful situations. The aim of this study was to investigate of resilience status among mothers of children on dialysis. Materials and methods: This descriptive cross-sectional study was performed on all mothers of children undergoing dialysis in Children's Medical Center, Mofid Hospital, Ali Asghar Hospital and Bahrami Hospital. A total of 166 eligible mothers were selected by census and completed a two-part questionnaire including demographic information and Connor-Davidson Resilience Scale. Data analysis was performed using SPSS software version 22 at a significance level of 0.05. Results: The mean of resilience was 3.35 out of 4 points and was at strong level. Also, the mean of dimensions of personal competence/strength, trust in personal instincts, tolerance of negative emotions, control and spirituality in individuals were 3.37, 3.17, 3.43, 3.45 and 3.33, respectively. The resilience of mothers of children undergoing hemodialysis was significantly higher than children under peritoneal dialysis (p=0.047). There was no significant association between maternal age, maternal education, maternal status of morbidity, child age, child gender, number of children in the family and duration of dialysis and mean maternal resilience. Conclusion: The resilience situation was at a good level. Due to the importance of resilience, it is recommended to provide educational and empowerment interventions for parents of children with chronic diseases.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77949850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Mesgarian, Termeh Tarjoman, M. Karimloo, Mahnaz Valizadeh, Zahra Hanifezadeh, O. Ameli, Mohsen Alijani, Behnam Farhoodi, M. Zangeneh
Background: Today, the coronavirus disease 2019 (COVID-19) pandemic and its control are the global priority. Therefore, identifying the epidemiological and clinical patterns of this disease is critical. We aimed to evaluate the clinical and epidemiological characteristics of patients diagnosed with COVID-19 and its relationship with disease severity. Materials and methods: In this cross- sectional study, we recruited patients referred to Amir-al-Momenin Hospital, Tehran, Iran, diagnosed with COVID-19 from February 20, 2020 to April 19, 2020. Data related to the clinical and epidemiological characteristics of COVID-19 admitted patients were collected through their hospital records and were analyzed using descriptive methods by ANOVA, Chi-square tests, multivariable logistic regression, and Tukey post hoc tests.
{"title":"Evaluation of clinical, epidemiological and paraclinical characteristics of patients diagnosed with COVID-19 and its relationship with disease severity in Amir Al-Momenin Hospital in Tehran","authors":"M. Mesgarian, Termeh Tarjoman, M. Karimloo, Mahnaz Valizadeh, Zahra Hanifezadeh, O. Ameli, Mohsen Alijani, Behnam Farhoodi, M. Zangeneh","doi":"10.52547/iau.32.1.64","DOIUrl":"https://doi.org/10.52547/iau.32.1.64","url":null,"abstract":"Background: Today, the coronavirus disease 2019 (COVID-19) pandemic and its control are the global priority. Therefore, identifying the epidemiological and clinical patterns of this disease is critical. We aimed to evaluate the clinical and epidemiological characteristics of patients diagnosed with COVID-19 and its relationship with disease severity. Materials and methods: In this cross- sectional study, we recruited patients referred to Amir-al-Momenin Hospital, Tehran, Iran, diagnosed with COVID-19 from February 20, 2020 to April 19, 2020. Data related to the clinical and epidemiological characteristics of COVID-19 admitted patients were collected through their hospital records and were analyzed using descriptive methods by ANOVA, Chi-square tests, multivariable logistic regression, and Tukey post hoc tests.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74785306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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