N. Bahador, Niusha Baseri Salehi, Paniz Baseri Salehi, Masoumeh Samimi, Mitra Adib
Worldwide epidemic had a specific role on human health and economic;Corona, for example, as a huge pandemic had an expand effect on human health, social relation, economic, politic and environment with severe respiratory disorder and high mortality rate in the world. Due to importance of SARS-Cov-2, the present paper deals with the route of transmission, their relation with sex, prevention and control. Research shows that sex-linked immunological differences as well as sex hormones are effective in responding to infectious diseases. Since COVID- 19 is a critical global treat, therefore, in this article, factors such as restricting populations, appropriate medicine, forestry, population growth control, global ban on wildlife trade and nutrition, which are important factors in the spread of this organism are discussed. Results illustrated thatsex hormones and presence of ACE2 receptors in male are the most important factors for developing the disease. Hence, it is recommended that they take more responsibility for the use of masks, regular hand washing with disinfectants, reducing the use of cigarettes and alcohol, and using foods containing vitamins such as A, C, E, zinc, Selenium and omega-3s and reduced presence in populations can prevent virus transmission.
{"title":"COVID- 19: disease, progress, control and their relation with sex","authors":"N. Bahador, Niusha Baseri Salehi, Paniz Baseri Salehi, Masoumeh Samimi, Mitra Adib","doi":"10.52547/iau.32.1.1","DOIUrl":"https://doi.org/10.52547/iau.32.1.1","url":null,"abstract":"Worldwide epidemic had a specific role on human health and economic;Corona, for example, as a huge pandemic had an expand effect on human health, social relation, economic, politic and environment with severe respiratory disorder and high mortality rate in the world. Due to importance of SARS-Cov-2, the present paper deals with the route of transmission, their relation with sex, prevention and control. Research shows that sex-linked immunological differences as well as sex hormones are effective in responding to infectious diseases. Since COVID- 19 is a critical global treat, therefore, in this article, factors such as restricting populations, appropriate medicine, forestry, population growth control, global ban on wildlife trade and nutrition, which are important factors in the spread of this organism are discussed. Results illustrated thatsex hormones and presence of ACE2 receptors in male are the most important factors for developing the disease. Hence, it is recommended that they take more responsibility for the use of masks, regular hand washing with disinfectants, reducing the use of cigarettes and alcohol, and using foods containing vitamins such as A, C, E, zinc, Selenium and omega-3s and reduced presence in populations can prevent virus transmission.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83414734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Chronic bipolar disorder (CBD) impairs cognition, leads to depression, and deteriorates patients’ quality of life. This study assessed the usefulness of RehaCom cognitive rehabilitation software on attention and working memory functions in patients with CBD. Materials and methods: This was a pre-test, post-test, quasi-experimental study. In this study, 50 patients with CBD at Mahan Rehabilitation Center, Tehran, Iran were selected using purposive sampling technique and were randomly divided into the control and intervention group. Both groups were pre-tested using Beck Depression Inventory, SDMT, PASAT, and n-BACK tests. The intervention group received 10 sessions (45 min each) of RehaCom software intervention. The participants in the control group were waiting-list controls. Data analyzed using descriptive statistics and multivariate analysis of covariance. Significance level was set at 0.05. Results: Cognitive rehabilitation by RehaCom software improved attention (F = 13.92, P = 0.006, = 0.53) and working memory (F = 43.58, P = 0.002, = 0.77) as compared to the control group. Conclusion: The results showed that cognitive rehabilitation program by RehaCom software can be used to improve attention function and working memory in patients with chronic bipolar disorder. Therefore, in order to use the software to improve cognitive functions, it is suggested that more detailed studies be conducted on a larger number of these types of patients.
背景:慢性双相情感障碍(CBD)损害认知，导致抑郁，并恶化患者的生活质量。本研究评估了RehaCom认知康复软件对CBD患者注意力和工作记忆功能的有效性。材料与方法:本研究为前测、后测、准实验研究。本研究采用目的抽样方法，选取伊朗德黑兰Mahan康复中心的50例CBD患者，随机分为对照组和干预组。两组均采用贝克抑郁量表、SDMT、PASAT和n-BACK测试进行预测。干预组接受10次RehaCom软件干预，每次45分钟。对照组的参与者是等候名单控制组。数据分析采用描述性统计和多变量协方差分析。显著性水平设为0.05。结果:采用RehaCom软件进行认知康复治疗，患者的注意力(F = 13.92, P = 0.006, 0.53)和工作记忆(F = 43.58, P = 0.002, 0.77)较对照组有明显改善。结论:采用RehaCom软件进行认知康复治疗可改善慢性双相情感障碍患者的注意功能和工作记忆。因此，为了使用该软件改善认知功能，建议对更多的这类患者进行更详细的研究。
{"title":"Cognitive rehabilitation of attention and working memory in patients with chronic bipolar disorder using RehaCom software","authors":"Atefe Saeb, Peyman Hassani-Abharian, Shahram Zarrabian","doi":"10.52547/iau.32.1.75","DOIUrl":"https://doi.org/10.52547/iau.32.1.75","url":null,"abstract":"Background: Chronic bipolar disorder (CBD) impairs cognition, leads to depression, and deteriorates patients’ quality of life. This study assessed the usefulness of RehaCom cognitive rehabilitation software on attention and working memory functions in patients with CBD. Materials and methods: This was a pre-test, post-test, quasi-experimental study. In this study, 50 patients with CBD at Mahan Rehabilitation Center, Tehran, Iran were selected using purposive sampling technique and were randomly divided into the control and intervention group. Both groups were pre-tested using Beck Depression Inventory, SDMT, PASAT, and n-BACK tests. The intervention group received 10 sessions (45 min each) of RehaCom software intervention. The participants in the control group were waiting-list controls. Data analyzed using descriptive statistics and multivariate analysis of covariance. Significance level was set at 0.05. Results: Cognitive rehabilitation by RehaCom software improved attention (F = 13.92, P = 0.006, = 0.53) and working memory (F = 43.58, P = 0.002, = 0.77) as compared to the control group. Conclusion: The results showed that cognitive rehabilitation program by RehaCom software can be used to improve attention function and working memory in patients with chronic bipolar disorder. Therefore, in order to use the software to improve cognitive functions, it is suggested that more detailed studies be conducted on a larger number of these types of patients.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75214555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Aboutalebi, Nafiseh Yazdchi, hadi Smkhani akbarinejhad
Background: Because multiple sclerosis (MS) affects both the body and the mind, in addition to pharmacological and physiological interventions, psychological interventions should be considered in the treatment of these patients. The aim of this study was to determine the effectiveness of compassion-focused therapy on death anxiety and quality of life in women with multiple sclerosis. Materials and methods: This study was a semi-experimental with a pre-test, post-test control group design. This study consisted of all women with MS in Esfahan who were members of the MS Society in 2019. In total, 30 women were selected and randomly divided into two groups by a purposive sampling method. Members of the experimental group received their treatment in eight sessions of 1.5 hours; however, the control group did not receive any treatment. Data were analyzed using ANOVA with repeated measures. Results: In the pre-test, mean and standard deviation of death anxiety score and quality of life were not significantly different in the experimental and control groups (p>0.05). Compassion-focused therapy reduced patients' death anxiety post-test, and increased their quality of life (p<0.001). At the follow-up stage, the effect of this treatment was lasting (p<0.001). Conclusion: Compassion-focused therapy can reduce death anxiety and thus better cope with the disease and increase the quality of life of women with MS.
{"title":"Effectiveness of compassion-focused therapy on death anxiety and quality of life among women with multiple sclerosis","authors":"H. Aboutalebi, Nafiseh Yazdchi, hadi Smkhani akbarinejhad","doi":"10.52547/iau.32.1.92","DOIUrl":"https://doi.org/10.52547/iau.32.1.92","url":null,"abstract":"Background: Because multiple sclerosis (MS) affects both the body and the mind, in addition to pharmacological and physiological interventions, psychological interventions should be considered in the treatment of these patients. The aim of this study was to determine the effectiveness of compassion-focused therapy on death anxiety and quality of life in women with multiple sclerosis. Materials and methods: This study was a semi-experimental with a pre-test, post-test control group design. This study consisted of all women with MS in Esfahan who were members of the MS Society in 2019. In total, 30 women were selected and randomly divided into two groups by a purposive sampling method. Members of the experimental group received their treatment in eight sessions of 1.5 hours; however, the control group did not receive any treatment. Data were analyzed using ANOVA with repeated measures. Results: In the pre-test, mean and standard deviation of death anxiety score and quality of life were not significantly different in the experimental and control groups (p>0.05). Compassion-focused therapy reduced patients' death anxiety post-test, and increased their quality of life (p<0.001). At the follow-up stage, the effect of this treatment was lasting (p<0.001). Conclusion: Compassion-focused therapy can reduce death anxiety and thus better cope with the disease and increase the quality of life of women with MS.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74266659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Shakerzadeh, M. Movahedin, A. Eidi, N. Hayati Roodbari, K. Parivar
Background: Mice are the most commonly used animal in reproductive research and following the urgent need for these type of studies and also due to the increased interest in the ethical principle of animal rights, four various inbred and outbred strains of the laboratory mouse were evaluated to select the more efficient one for reproductive research. Materials and methods: 60 female and 16 male of strains (C57, CD1, NMRI, and Balb/c) weighing 25 to 30g and aged 6 to 8 weeks were evaluated under same conditions at different stages of mature oocyte collection, fertilization and in vitro embryo development up to the blastocyst stage. The data were analyzed using a chi-square test, and the selected significance level was p<0.05. Results: Among the four strains, the highest to lowest fetal survival rates were for the CD1, NMRI, Balb /C and C57 mice, respectively and their values were 38.9, 14.4, 9.1 and 3.1%, individually. Conclusion: Considering the results, we conclude that it is not possible to obtain optimal results for some strains due to using same instructions. The results showed that the highest rate of fertilization and embryo development up to the 8-cell stage was observed in the outbred CD1 mice. It seems that this strain is more applicable than others for reproductive research. In addition, we believe that using different medium during fertilization and embryo development as well as laboratory conditions, probably assist in improving the embryo production while minimized the required number of animals and allowed the achievement of the desired result.
{"title":"Evaluation of different strain mice during in vitro fertilization and embryo development in response to superovulation","authors":"J. Shakerzadeh, M. Movahedin, A. Eidi, N. Hayati Roodbari, K. Parivar","doi":"10.52547/iau.32.1.53","DOIUrl":"https://doi.org/10.52547/iau.32.1.53","url":null,"abstract":"Background: Mice are the most commonly used animal in reproductive research and following the urgent need for these type of studies and also due to the increased interest in the ethical principle of animal rights, four various inbred and outbred strains of the laboratory mouse were evaluated to select the more efficient one for reproductive research. Materials and methods: 60 female and 16 male of strains (C57, CD1, NMRI, and Balb/c) weighing 25 to 30g and aged 6 to 8 weeks were evaluated under same conditions at different stages of mature oocyte collection, fertilization and in vitro embryo development up to the blastocyst stage. The data were analyzed using a chi-square test, and the selected significance level was p<0.05. Results: Among the four strains, the highest to lowest fetal survival rates were for the CD1, NMRI, Balb /C and C57 mice, respectively and their values were 38.9, 14.4, 9.1 and 3.1%, individually. Conclusion: Considering the results, we conclude that it is not possible to obtain optimal results for some strains due to using same instructions. The results showed that the highest rate of fertilization and embryo development up to the 8-cell stage was observed in the outbred CD1 mice. It seems that this strain is more applicable than others for reproductive research. In addition, we believe that using different medium during fertilization and embryo development as well as laboratory conditions, probably assist in improving the embryo production while minimized the required number of animals and allowed the achievement of the desired result.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82033344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nazanin Baghaipour, M. Mohammadi, Yasamin Khosravani-Nezhad
Background: Vitamin D deficiency is related to acute myocardial infarction (MI) directly and indirectly. Therefore, this study evaluated serum level of vitamin D in patients with acute MI. Materials and methods: This is a cross-sectional and descriptive study on 84 patients with acute MI in Amiralmomenin hospital since May 2018 to May 2019. The demographic information of patients including risk factors for MI as: age, gender, body mass index, hypertension, hyperlipidemia, diabetes, cigarettes use or drugs abuse, history of cardiovascular diseases, inpatient time, and vitamin D status are gathered as questionnaires. The data was added to SPSS version 25, and the P-value of 0.05 was considered significant. Results: 52.4% of patients were female. All the patients were 36 to 90 years old, and the mean (±standard deviation) age was 67.51±12.90 years old. 25% of the patients had vitamin D deficiency, 23.8% insufficiency, 11.9% severe deficiency, and 51.2% normal value. However, none of the risk factors were related to the vitamin D status significantly. Conclusion: In this study, none of the risk factors were related to vitamin D status in MI patients, yet cohort studies with major samples are required because there are several studies confirm that vitamin D status has an important role in development of acute MI.
{"title":"Evaluation of serum level of vitamin D in patients with acute myocardial infarction","authors":"Nazanin Baghaipour, M. Mohammadi, Yasamin Khosravani-Nezhad","doi":"10.52547/iau.32.1.102","DOIUrl":"https://doi.org/10.52547/iau.32.1.102","url":null,"abstract":"Background: Vitamin D deficiency is related to acute myocardial infarction (MI) directly and indirectly. Therefore, this study evaluated serum level of vitamin D in patients with acute MI. Materials and methods: This is a cross-sectional and descriptive study on 84 patients with acute MI in Amiralmomenin hospital since May 2018 to May 2019. The demographic information of patients including risk factors for MI as: age, gender, body mass index, hypertension, hyperlipidemia, diabetes, cigarettes use or drugs abuse, history of cardiovascular diseases, inpatient time, and vitamin D status are gathered as questionnaires. The data was added to SPSS version 25, and the P-value of 0.05 was considered significant. Results: 52.4% of patients were female. All the patients were 36 to 90 years old, and the mean (±standard deviation) age was 67.51±12.90 years old. 25% of the patients had vitamin D deficiency, 23.8% insufficiency, 11.9% severe deficiency, and 51.2% normal value. However, none of the risk factors were related to the vitamin D status significantly. Conclusion: In this study, none of the risk factors were related to vitamin D status in MI patients, yet cohort studies with major samples are required because there are several studies confirm that vitamin D status has an important role in development of acute MI.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"140 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77748662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Arezoo Nazari, M. Mohammadi, Masoumeh Nezhadali Lamfjani, T. Naji
Background: Diabetes is one of the most common chronic metabolic diseases in the world that is caused by decreased insulin secretion or insulin resistance and fat accumulation in visceral adipose tissue (IR). Omentin is a protein inferred from adipose tissue that is associated with the rate of diabetes. The aim of this study was to investigate the relationship between rs2274907 polymorphism of amentine-1 gene with diabetes. Materials and methods: This case-control study was conducted on 70 patients with diabetes and 70 healthy individuals as control. FBS, TG, CHOL, HDL, and LDL were measured in the subjects and genotype determination was performed using PCR-RFLP. Results: Age, body mass index, LDL, cholesterol, triglyceride, fasting high sugar and insulin resistance were significantly different between patients and healthy individuals (p<0.05). No significant difference was observed in omentin level in diabetic compared to control patients (p>0.05). Omentin levels in different genotypes rs2274907 were different in healthy subjects, but the difference was not significant. Omentin levels in different genotypes rs2274907 were different in healthy subjects (p<0.05). Genotype regression analysis showed that genotypes in rs2274907 polymorphism were not related to diabetes (p>0.05). Conclusion: This study revealed that there was no significant relationship between rs2274907 genotype and type 2 diabetes.
{"title":"Association of rs2274907 polymorphism of omentin gene with type 2 diabetes in Iranian population","authors":"Arezoo Nazari, M. Mohammadi, Masoumeh Nezhadali Lamfjani, T. Naji","doi":"10.52547/iau.32.1.44","DOIUrl":"https://doi.org/10.52547/iau.32.1.44","url":null,"abstract":"Background: Diabetes is one of the most common chronic metabolic diseases in the world that is caused by decreased insulin secretion or insulin resistance and fat accumulation in visceral adipose tissue (IR). Omentin is a protein inferred from adipose tissue that is associated with the rate of diabetes. The aim of this study was to investigate the relationship between rs2274907 polymorphism of amentine-1 gene with diabetes. Materials and methods: This case-control study was conducted on 70 patients with diabetes and 70 healthy individuals as control. FBS, TG, CHOL, HDL, and LDL were measured in the subjects and genotype determination was performed using PCR-RFLP. Results: Age, body mass index, LDL, cholesterol, triglyceride, fasting high sugar and insulin resistance were significantly different between patients and healthy individuals (p<0.05). No significant difference was observed in omentin level in diabetic compared to control patients (p>0.05). Omentin levels in different genotypes rs2274907 were different in healthy subjects, but the difference was not significant. Omentin levels in different genotypes rs2274907 were different in healthy subjects (p<0.05). Genotype regression analysis showed that genotypes in rs2274907 polymorphism were not related to diabetes (p>0.05). Conclusion: This study revealed that there was no significant relationship between rs2274907 genotype and type 2 diabetes.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85022053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sahar Abbasi Geravand, R. Khajavi, M. Rahimi, Manouchehr Shamsini Ghiyasvand, A. Meftahi
Background: Nanomicrobial cellulose is an important biopolymer with a three-dimensional structure that is produced by some microorganisms and has been widely used in medicine. One of the unique properties of microbial cellulose is its very high water absorption, which can be used to produce modern wound dressings. But after drying, it’s three-dimensional structure collapses and the amount of water reabsorption decreases. Accordingly, the aim of this project was to preserve the three-dimensional structure of nanomicrobial cellulose by networking it and improving the water reabsorption properties of this biopolymer. In addition, the cell viability, proliferation, and cell growth of the modified structure and untreated microbial cellulose were also studied. Materials and methods: In this study, microbial cellulose was produced, purified, and neutralized using stationary culture. The samples were then treated with different concentrations of citric acid/sodium hypophosphite and crosslinked. Finally, the characteristics of treated and raw samples were studied by various tests including ATR-FTIR, MTT, SEM, water absorption and in vitro and in vitro tests. Results: According to the results, it was found that the cross-linking operation prevents the collapse of the structure and not only does not cause toxicity, but in addition to increasing water uptake, it also increases viability, adhesion, and cell proliferation in the modified cellulose. Conclusion: Cross-linked nanomicrobial cellulose has high potential as a modern wound dressing.
{"title":"Investigation on cross-linked nanomicrobial cellulose properties as modern wound dressing","authors":"Sahar Abbasi Geravand, R. Khajavi, M. Rahimi, Manouchehr Shamsini Ghiyasvand, A. Meftahi","doi":"10.52547/iau.32.1.11","DOIUrl":"https://doi.org/10.52547/iau.32.1.11","url":null,"abstract":"Background: Nanomicrobial cellulose is an important biopolymer with a three-dimensional structure that is produced by some microorganisms and has been widely used in medicine. One of the unique properties of microbial cellulose is its very high water absorption, which can be used to produce modern wound dressings. But after drying, it’s three-dimensional structure collapses and the amount of water reabsorption decreases. Accordingly, the aim of this project was to preserve the three-dimensional structure of nanomicrobial cellulose by networking it and improving the water reabsorption properties of this biopolymer. In addition, the cell viability, proliferation, and cell growth of the modified structure and untreated microbial cellulose were also studied. Materials and methods: In this study, microbial cellulose was produced, purified, and neutralized using stationary culture. The samples were then treated with different concentrations of citric acid/sodium hypophosphite and crosslinked. Finally, the characteristics of treated and raw samples were studied by various tests including ATR-FTIR, MTT, SEM, water absorption and in vitro and in vitro tests. Results: According to the results, it was found that the cross-linking operation prevents the collapse of the structure and not only does not cause toxicity, but in addition to increasing water uptake, it also increases viability, adhesion, and cell proliferation in the modified cellulose. Conclusion: Cross-linked nanomicrobial cellulose has high potential as a modern wound dressing.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79086020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: In this study, we aimed to evaluate the effects of GW9508 as an unsaturated fatty acid on inducing oxidative stress in HT-29 cancer cell line and non-cancer HUVEC cells. Materials and methods: The effects of GW9508 on cell viability were determined by performing MTT assay after 1, 3 and 5 days. The anti-oxidant superoxide dismutase (SOD) and catalase enzymes' assay was performed to evaluate the apoptosis. Moreover, inverted microscope and DAPI staining were applied to measure the changes in cell and nucleus morphology. Results: The IC50 dose of GW9508 was 500 μM in both cancer and normal cells. Results of enzymatic activity assay exhibited the increscent of activity of catalase and SOD in treated cells led to promoting apoptosis in cells. The amount of activities of SOD and catalase in HT-29 treated cells that were calculated 1.254 and 0.338 mU/mg respectively, which had a significant increase compared to activities of these two enzymes in control cells (0.85 and 0.206 mU/mg, respectively) (p≤0.05). There was also a significant increase in the activities of SOD and catalase in HUVEC treated cells (1.111 and 0.517 mU/mg) compared to the activities of the two enzymes in the control group, which were calculated 0.755 and 0.184 mU/mg, respectively. Conclusion: GW9508 small molecule induces oxidative stress and ROS production in HT-29 and HUVEC cells, leading to cytotoxicity and thus promoting apoptotic cell death in cancer cells. However, these effects are contingent upon dose and the type of cell lineages.
{"title":"Effects of GW9508 small molecule on oxidative stress enzymes in colorectal cancer and non-cancerous HUVEC cells","authors":"Behnoosh Rafienia, E. Hoveizi, A. Shahriari","doi":"10.52547/iau.32.1.31","DOIUrl":"https://doi.org/10.52547/iau.32.1.31","url":null,"abstract":"Background: In this study, we aimed to evaluate the effects of GW9508 as an unsaturated fatty acid on inducing oxidative stress in HT-29 cancer cell line and non-cancer HUVEC cells. Materials and methods: The effects of GW9508 on cell viability were determined by performing MTT assay after 1, 3 and 5 days. The anti-oxidant superoxide dismutase (SOD) and catalase enzymes' assay was performed to evaluate the apoptosis. Moreover, inverted microscope and DAPI staining were applied to measure the changes in cell and nucleus morphology. Results: The IC50 dose of GW9508 was 500 μM in both cancer and normal cells. Results of enzymatic activity assay exhibited the increscent of activity of catalase and SOD in treated cells led to promoting apoptosis in cells. The amount of activities of SOD and catalase in HT-29 treated cells that were calculated 1.254 and 0.338 mU/mg respectively, which had a significant increase compared to activities of these two enzymes in control cells (0.85 and 0.206 mU/mg, respectively) (p≤0.05). There was also a significant increase in the activities of SOD and catalase in HUVEC treated cells (1.111 and 0.517 mU/mg) compared to the activities of the two enzymes in the control group, which were calculated 0.755 and 0.184 mU/mg, respectively. Conclusion: GW9508 small molecule induces oxidative stress and ROS production in HT-29 and HUVEC cells, leading to cytotoxicity and thus promoting apoptotic cell death in cancer cells. However, these effects are contingent upon dose and the type of cell lineages.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79463317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Due to the importance of investigating nosocomial infections and drug resistance in bacteria causing these infections, the use of herbs can be very helpful in treatment. In the present study, the effect of silver nanoparticles obtained from portulaca oleracea extract on bacteria causing nosocomial infections and effective parameters in the synthesis of these nanoparticles were investigated. Materials and methods: First, an aqueous extract of portulaca oleracea was added to a 1 mM silver nitrate solution. The extract was used as a reducing and stabilizing agent for nanoparticles. The effect of effective parameters for the optimization of silver nanoparticles was evaluated by ultraviolet-visible spectroscopy and transmission electron microscopy. The antioxidant and antibacterial effect of the synthesized nanoparticles was determined by studying the activity of catalase, MIC, MBC, disk, and well method. Results: The results showed the effect of alkaline pH as well as silver salt concentration on the quality of synthesized nanoparticles. Also, the results of the antimicrobial test showed that gram-negative bacteria revealed significant sensitivity to synthetic nanoparticles and Staphylococcus aureus showed the highest resistance to nanoparticles. Antioxidant properties were also observed against gram-negative strains. Conclusion: The results showed that silver nanoparticles synthesized with portulaca oleracea had antibacterial and antioxidant properties. Therefore, photosynthesis of nanoparticles can be a good option for the treatment of infectious diseases caused by gram-negative bacteria.
{"title":"Phytosynthesis of silver nanoparticles with the aqueous extract of portulaca oleracea and its antimicrobial and antioxidant effect against gram-positive and gram-negative bacteria","authors":"N. Elyasi, S. Nabizadeh, Z. Shafiei","doi":"10.52547/iau.32.1.21","DOIUrl":"https://doi.org/10.52547/iau.32.1.21","url":null,"abstract":"Background: Due to the importance of investigating nosocomial infections and drug resistance in bacteria causing these infections, the use of herbs can be very helpful in treatment. In the present study, the effect of silver nanoparticles obtained from portulaca oleracea extract on bacteria causing nosocomial infections and effective parameters in the synthesis of these nanoparticles were investigated. Materials and methods: First, an aqueous extract of portulaca oleracea was added to a 1 mM silver nitrate solution. The extract was used as a reducing and stabilizing agent for nanoparticles. The effect of effective parameters for the optimization of silver nanoparticles was evaluated by ultraviolet-visible spectroscopy and transmission electron microscopy. The antioxidant and antibacterial effect of the synthesized nanoparticles was determined by studying the activity of catalase, MIC, MBC, disk, and well method. Results: The results showed the effect of alkaline pH as well as silver salt concentration on the quality of synthesized nanoparticles. Also, the results of the antimicrobial test showed that gram-negative bacteria revealed significant sensitivity to synthetic nanoparticles and Staphylococcus aureus showed the highest resistance to nanoparticles. Antioxidant properties were also observed against gram-negative strains. Conclusion: The results showed that silver nanoparticles synthesized with portulaca oleracea had antibacterial and antioxidant properties. Therefore, photosynthesis of nanoparticles can be a good option for the treatment of infectious diseases caused by gram-negative bacteria.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75586477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Afsaneh Mostagim Ahmadabadi, F. Sahbaeiroy, S. Hejazi
Background: The incidence of neural bladder is increasing today and these people need catheterization. Intermittent clean urinary catheterization is a low-risk method for the patients. Materials and methods: This was a descriptive methodological and validation research. For content validity, 10 people were selected by purposive method. For face validity, 25 people were chosen by convenience method Aimed at structural validity, gradual method was used from sounding conductors. For the reliability of the questionnaire, 10 people completed the questionnaire. Results: First, a draft was developed and two qualitative and quantitative methods were used for face validity. The effect scores of the items were not less than 1.5. None of the items less than 62% were deleted according to the table. In construct validity, exploratory factor analysis and varimax rotation methods were used and the adequacy of sample size was 0.78 by KMO test, and the results of Bartlett sphericity test were significant (4017/486 p <0.001). 5 items with a share of less than 0.4 were removed and factor analysis was performed again and the number of factors was reduced to four with a variance of 40.136. The results of internal consistency of Cronbach's alpha coefficient of the whole questionnaire with 32 items were equal to 0.862 and 0.859 for women and men respectively. The results of the stability of the verification tools and the result of the ICC test (0.949) showed a good correlation coefficient for each structure. Conclusion: The tools designed in this study are valid and reliable and can be used in various studies.
背景:神经性膀胱的发病率呈上升趋势，需要导尿。间歇清洁导尿是一种低风险的方法。材料和方法:本研究为描述性方法学和验证性研究。内容效度采用目的法选取10人。对于面孔效度，采用便利法选取25人;对于结构效度，采用从测音者开始的渐进法。为保证问卷的信度，共10人完成问卷。结果:首先，制定了一份草案，并采用了定性和定量两种方法进行了人脸效度测试。各题效分均不低于1.5分。根据该表，小于62%的项均未被删除。结构效度采用探索性因子分析和变量旋转法，KMO检验的样本量充分性为0.78,Bartlett球形检验的结果具有显著性(4017/486 p <0.001)。剔除份额小于0.4的5项，重新进行因子分析，将因子数量减少到4个，方差为40.136。整个32项问卷的Cronbach’s alpha系数的内部一致性结果，女性为0.862，男性为0.859。验证工具的稳定性与ICC检验结果(0.949)表明，各结构均具有良好的相关系数。结论:本研究设计的工具有效可靠，可用于各种研究。
{"title":"Psychometrics of the self-efficacy instrument of clean intermittent urinary catheterization in patients with neural bladder admitted to rehabilitation hospitals in Tehran in 2019","authors":"Afsaneh Mostagim Ahmadabadi, F. Sahbaeiroy, S. Hejazi","doi":"10.52547/iau.31.4.450","DOIUrl":"https://doi.org/10.52547/iau.31.4.450","url":null,"abstract":"Background: The incidence of neural bladder is increasing today and these people need catheterization. Intermittent clean urinary catheterization is a low-risk method for the patients. Materials and methods: This was a descriptive methodological and validation research. For content validity, 10 people were selected by purposive method. For face validity, 25 people were chosen by convenience method Aimed at structural validity, gradual method was used from sounding conductors. For the reliability of the questionnaire, 10 people completed the questionnaire. Results: First, a draft was developed and two qualitative and quantitative methods were used for face validity. The effect scores of the items were not less than 1.5. None of the items less than 62% were deleted according to the table. In construct validity, exploratory factor analysis and varimax rotation methods were used and the adequacy of sample size was 0.78 by KMO test, and the results of Bartlett sphericity test were significant (4017/486 p <0.001). 5 items with a share of less than 0.4 were removed and factor analysis was performed again and the number of factors was reduced to four with a variance of 40.136. The results of internal consistency of Cronbach's alpha coefficient of the whole questionnaire with 32 items were equal to 0.862 and 0.859 for women and men respectively. The results of the stability of the verification tools and the result of the ICC test (0.949) showed a good correlation coefficient for each structure. Conclusion: The tools designed in this study are valid and reliable and can be used in various studies.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89691057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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