Golnaz Faezi, M. S. Moghadam, S. - Shadmehri, M. Fathalipour
Background: The pathway of mTORC1 is one of the most important pathway in protein synthesis, and type 2 diabetes and insulin resistance can lead to inhibit this pathway. The aim of this study was to investigate the effect of 4 weeks high-intensity interval training (HIIT) on the content of downstream and upstream mTORC1 pathways in gastrocnemius muscle in type 2 diabetic rats. Materials and methods: In this experimental study, 16 Sprague-Dawley male rats (with mean weight of 250 ± 20 gr) were selected and after induction of diabetes by streptozotocin and nicotinamide, the rats were randomly assigned into two groups, including diabetic HIIT training and diabetic control. The experimental group performed 4 days a week the exercise training for 4 weeks, while the control group did not have any training program. Independent T-test was used to analyze the data. Results: A significant change was not observed in the total content of AKT1 proteins (p<0.31), P70S6K1 (p<0.69) and 4EBP1 (p<0.84) in the HIIT training group compared to the control group, but the total protein content of mTOR (p<0.02) and the form of phosphorylation of AKT1 (p<0.03), mTOR (p<0.03), P70S6K1 (p<0.02) and 4EBP1 (p<0.009) proteins showed significant increase in training group compared to the control group. Conclusion: The HIIT training can probably activate the pathway of AKT1/mTOR/P70S6K1 and AKT1/mTOR/4EBP1 in the mTORC1 pathway; therefore, the HIIT training can lead to protein synthesis or muscle hypertrophy through this pathway in the gastrocnemius muscle of the trained rats
{"title":"The effect of 4 weeks high-intensity interval training (HIIT) on the content of downstream and upstream mTORC1 pathways gastrocnemius muscle of type 2 diabetic rats","authors":"Golnaz Faezi, M. S. Moghadam, S. - Shadmehri, M. Fathalipour","doi":"10.29252/iau.30.2.120","DOIUrl":"https://doi.org/10.29252/iau.30.2.120","url":null,"abstract":"Background: The pathway of mTORC1 is one of the most important pathway in protein synthesis, and type 2 diabetes and insulin resistance can lead to inhibit this pathway. The aim of this study was to investigate the effect of 4 weeks high-intensity interval training (HIIT) on the content of downstream and upstream mTORC1 pathways in gastrocnemius muscle in type 2 diabetic rats. Materials and methods: In this experimental study, 16 Sprague-Dawley male rats (with mean weight of 250 ± 20 gr) were selected and after induction of diabetes by streptozotocin and nicotinamide, the rats were randomly assigned into two groups, including diabetic HIIT training and diabetic control. The experimental group performed 4 days a week the exercise training for 4 weeks, while the control group did not have any training program. Independent T-test was used to analyze the data. Results: A significant change was not observed in the total content of AKT1 proteins (p<0.31), P70S6K1 (p<0.69) and 4EBP1 (p<0.84) in the HIIT training group compared to the control group, but the total protein content of mTOR (p<0.02) and the form of phosphorylation of AKT1 (p<0.03), mTOR (p<0.03), P70S6K1 (p<0.02) and 4EBP1 (p<0.009) proteins showed significant increase in training group compared to the control group. Conclusion: The HIIT training can probably activate the pathway of AKT1/mTOR/P70S6K1 and AKT1/mTOR/4EBP1 in the mTORC1 pathway; therefore, the HIIT training can lead to protein synthesis or muscle hypertrophy through this pathway in the gastrocnemius muscle of the trained rats","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78323539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: In recent years, the unique physical and chemical properties of carbon nanostructures has led to many advancements in various fields, including chemistry and pharmaceuticals. Graphene is one of the carbon nanostructures which have attracted significant attention from researchers in adsorption and release of various drugs. Due to the high surface area of graphene, it can be used as a biological carrier in drug delivery. In this study, the interaction of aspirin with a graphene sheet doped with aluminum (graphenealuminum) and possibility of stable complex formation between them were investigated using the theoretical study. Materials and methods: The performance of carbon nanostructures for adsorption of aspirin on graphenealuminum was evaluated using quantum computation. The calculations were performed using density functional theory modified with dispersion forces (DFT-D) and basic functions by using of ORCA software. Results: Adsorption energy and electronic structure of aspirin /graphene-aluminum system were calculated. The measured adsorption energy and bond distance were −53.08 (kcal/mol) and 1.888 Å, respectively. The distribution of electron charge also indicated the continuity of electron clouds between drugs and nanostructure. Conclusion: The results showed that a strong bond formed between aspirin and graphene-aluminum and the complex formed in the aqueous medium was thermodynamically stable. Regarding the possibility of stable complex formation, graphene-aluminum was expected to be suitable nanocarier for delivery of aspirin to target cells.
{"title":"Theoretical study of interaction between aspirine drug and Al-soped graphene nanostructure toward designing of suitable nanocarrier for drug delivery","authors":"سارا فرشاد, مسعود درویش گنجی","doi":"10.29252/iau.30.2.141","DOIUrl":"https://doi.org/10.29252/iau.30.2.141","url":null,"abstract":"Background: In recent years, the unique physical and chemical properties of carbon nanostructures has led to many advancements in various fields, including chemistry and pharmaceuticals. Graphene is one of the carbon nanostructures which have attracted significant attention from researchers in adsorption and release of various drugs. Due to the high surface area of graphene, it can be used as a biological carrier in drug delivery. In this study, the interaction of aspirin with a graphene sheet doped with aluminum (graphenealuminum) and possibility of stable complex formation between them were investigated using the theoretical study. Materials and methods: The performance of carbon nanostructures for adsorption of aspirin on graphenealuminum was evaluated using quantum computation. The calculations were performed using density functional theory modified with dispersion forces (DFT-D) and basic functions by using of ORCA software. Results: Adsorption energy and electronic structure of aspirin /graphene-aluminum system were calculated. The measured adsorption energy and bond distance were −53.08 (kcal/mol) and 1.888 Å, respectively. The distribution of electron charge also indicated the continuity of electron clouds between drugs and nanostructure. Conclusion: The results showed that a strong bond formed between aspirin and graphene-aluminum and the complex formed in the aqueous medium was thermodynamically stable. Regarding the possibility of stable complex formation, graphene-aluminum was expected to be suitable nanocarier for delivery of aspirin to target cells.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79464631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Nursing in intensive care units is associated with various stressors that can affect the psychological state. In this study, the effect of positive thinking training on the happiness and resiliency of nurses in intensive care units was investigated. Materials and methods: A total of 60 nurses from Shohadaye Tajrish and Shahid Taleghani hospitals of Tehran City were randomly divided into two groups. The intervention group received teaching positive thinking skills for 8 sessions (each session was 1.5 hours). Data were collected using a three-part questionnaire on demographic data, Oxford Happiness Index (OHI), and Connor-Davidson Resilience Scale (CD-RISC). Data were analyzed using SPSS version 23 software. Results: Demographic variables had no significant differences between two groups. There was no significant difference in the mean of happiness and resiliency in the pre-test stage between two groups. The results of covariance analysis indicated the effectiveness of intervention in increasing happiness and resiliency, so that 55% of changes in variance of happiness and 43% of changes in variance of resiliency were due to independent variable. Conclusion: Training of positive thinking skills improved nurses' happiness and resilience. These interventions are an uncomplicated and effective method that can be used extensively in nurses to improve psychological components.
{"title":"The effect of positive thinking training on happiness and resilience of nurses in intensive care units: a randomized clinical trial","authors":"پانته آ کیانی, سید محمد کاظم نائینی, محبوبه صفوی","doi":"10.29252/iau.30.2.193","DOIUrl":"https://doi.org/10.29252/iau.30.2.193","url":null,"abstract":"Background: Nursing in intensive care units is associated with various stressors that can affect the psychological state. In this study, the effect of positive thinking training on the happiness and resiliency of nurses in intensive care units was investigated. Materials and methods: A total of 60 nurses from Shohadaye Tajrish and Shahid Taleghani hospitals of Tehran City were randomly divided into two groups. The intervention group received teaching positive thinking skills for 8 sessions (each session was 1.5 hours). Data were collected using a three-part questionnaire on demographic data, Oxford Happiness Index (OHI), and Connor-Davidson Resilience Scale (CD-RISC). Data were analyzed using SPSS version 23 software. Results: Demographic variables had no significant differences between two groups. There was no significant difference in the mean of happiness and resiliency in the pre-test stage between two groups. The results of covariance analysis indicated the effectiveness of intervention in increasing happiness and resiliency, so that 55% of changes in variance of happiness and 43% of changes in variance of resiliency were due to independent variable. Conclusion: Training of positive thinking skills improved nurses' happiness and resilience. These interventions are an uncomplicated and effective method that can be used extensively in nurses to improve psychological components.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80156525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Jamali-Raeufy, M. Zeinivand, Mina Goudarzi, Sobhan Haghani
Background: Temporal lobe epilepsy is a chronic neurological disorder characterized by spontaneous seizures, learning and memory deficiency, loss of neurons, mossy fiber sprouting and tissue apoptosis. This study was to investigate the effect of NOP receptor agonist (MCOPPB) and antagonist (SB612111) on seizure and cognitive dysfunction and histological studies in experimental model of temporal lobe epilepsy in male rat. Materials and methods: in this study, 50 male rats were divided into six groups, including sham, epileptic, valperoic treated epileptic, NOP receptor agonist and antagonist treated epileptic. Finally, seizure behavior, short-term (Y-maze) and long-term (shuttle box) memory, GFAP value and also histologic finding (Nissel, Tim and Apoptosis staining) were evaluated. Results: Kinic acid induced seizures associated with significant seizure behavior, impairment of learning and memory and tissue damage. Pretreated epileptic rats with NOP receptor agonist decreased seizure attacks, but did not improve memory. Administration of NOP antagonist was not effective on the seizure behavior, but contribute to improve the memory and learning abilities following treatment. Also, administration of NOP agonist and antagonist increased neuron count, reduced increased sprouting of mossy fibers, cell death and the activity of astrocytes in the hippocampus. Conclusion: pre-treatment of epileptic rets with NOP receptor agonist and antagonist reduced seizures attacks and improved short-term spatial memory and tissue damage in rats.
{"title":"The effect of agonist and antagonist of Nociceptine/Orphanin FQ receptor on seizure and cognitive dysfunction in experimental model of temporal lobe epilepsy in male rat","authors":"N. Jamali-Raeufy, M. Zeinivand, Mina Goudarzi, Sobhan Haghani","doi":"10.29252/iau.30.2.107","DOIUrl":"https://doi.org/10.29252/iau.30.2.107","url":null,"abstract":"Background: Temporal lobe epilepsy is a chronic neurological disorder characterized by spontaneous seizures, learning and memory deficiency, loss of neurons, mossy fiber sprouting and tissue apoptosis. This study was to investigate the effect of NOP receptor agonist (MCOPPB) and antagonist (SB612111) on seizure and cognitive dysfunction and histological studies in experimental model of temporal lobe epilepsy in male rat. Materials and methods: in this study, 50 male rats were divided into six groups, including sham, epileptic, valperoic treated epileptic, NOP receptor agonist and antagonist treated epileptic. Finally, seizure behavior, short-term (Y-maze) and long-term (shuttle box) memory, GFAP value and also histologic finding (Nissel, Tim and Apoptosis staining) were evaluated. Results: Kinic acid induced seizures associated with significant seizure behavior, impairment of learning and memory and tissue damage. Pretreated epileptic rats with NOP receptor agonist decreased seizure attacks, but did not improve memory. Administration of NOP antagonist was not effective on the seizure behavior, but contribute to improve the memory and learning abilities following treatment. Also, administration of NOP agonist and antagonist increased neuron count, reduced increased sprouting of mossy fibers, cell death and the activity of astrocytes in the hippocampus. Conclusion: pre-treatment of epileptic rets with NOP receptor agonist and antagonist reduced seizures attacks and improved short-term spatial memory and tissue damage in rats.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78621989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Shahrasbi, Kosar Heydarzadeh, Sepideh Fahimi, Nazila Abbasi fard
Paraneoplastic syndromes are generally defined as clinical disorder associated with malignant diseases, and hypocalcemia as paraneoplastic manifestation associated with cancer is a rare condition. In patient with bone metastatic cancer, osteolytic lesions are more common than osteoblastic ones which give rise to hypercalcemia and hypocalcemia is a rare condition in this group of patients. We described a 56-year-old male with the history of gastric cancer and gastrectomy and chemotherapy since one year ego who came to clinic with complaints of nausea, vomiting and paresthesia of extremities which after diagnostic work up, paraneoplastic hypocalcemia was diagnosed. Hypocalcemia following bone metastasis is not a common phenomenon, but in patients with the history of gastric cancer and neurological symptoms (paresthesia, ...) evaluation of serum calcium value is recommended.
{"title":"Paraneoplastic hypocalcemia in a patient whit gastric cancer accompanied by osteoblastic metastasis","authors":"A. Shahrasbi, Kosar Heydarzadeh, Sepideh Fahimi, Nazila Abbasi fard","doi":"10.29252/iau.30.2.221","DOIUrl":"https://doi.org/10.29252/iau.30.2.221","url":null,"abstract":"Paraneoplastic syndromes are generally defined as clinical disorder associated with malignant diseases, and hypocalcemia as paraneoplastic manifestation associated with cancer is a rare condition. In patient with bone metastatic cancer, osteolytic lesions are more common than osteoblastic ones which give rise to hypercalcemia and hypocalcemia is a rare condition in this group of patients. We described a 56-year-old male with the history of gastric cancer and gastrectomy and chemotherapy since one year ego who came to clinic with complaints of nausea, vomiting and paresthesia of extremities which after diagnostic work up, paraneoplastic hypocalcemia was diagnosed. Hypocalcemia following bone metastasis is not a common phenomenon, but in patients with the history of gastric cancer and neurological symptoms (paresthesia, ...) evaluation of serum calcium value is recommended.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"86 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79370260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Dinarvand, M. Hashemi, Rasool Dinarvand, Shabnam Movaseghi, Mojtaba Jafarinia
Background : Most drugs used by pregnant women can pass through the placenta and expose embryos and developing embryos to teratogenic effects. Due to the increased cellular damage of Bax gene expression, the effect of cholorodiazepoxide use during pregnancy on Bax gene expression in the hippocampus of neonatal rats was investigated. Materials and methods : After confirmation of pregnancy in Wistar female rats, intraperitoneal injection of chlordiazepoxide was administered at a dose of 10 mg/ kg daily for 21 days in the experimental group. Two weeks after birth, the infant's brain was removed from the skull. After isolating the CA1 region of the neonatal hippocampus, the expression of the Bax proapoptotic gene was examined and compared with the control and carrier group (saline). Results : The level of gene expression was analyzed and propoptotic Bax gene showed significant increase in experimental group compared to control group. Conclusion : The results of this study indicated that the administration of chlorodiazepoxide during pregnancy can cause neuronal damage in the hippocampus of Wistar rats.
背景:孕妇使用的大多数药物可以通过胎盘，使胚胎和发育中的胚胎暴露于致畸作用中。由于Bax基因表达的细胞损伤增加，我们研究了妊娠期氯二氮环氧化物对新生大鼠海马Bax基因表达的影响。材料与方法:实验组Wistar雌性大鼠确认妊娠后，以10 mg/ kg / d的剂量腹腔注射氯二氮环氧乙烷，连续注射21 d。出生两周后，婴儿的大脑从头骨中取出。分离新生海马CA1区后，检测Bax促凋亡基因的表达，并与对照组和载体组(生理盐水)进行比较。结果:对基因表达水平进行分析，与对照组相比，实验组促生Bax基因表达水平明显升高。结论:本研究提示妊娠期给药氯二氮环氧化物可引起Wistar大鼠海马神经元损伤。
{"title":"The effects of chlorodiazepoxide on the Bax gene expression in CA1 pyramidal cells of the newborn rat hippocampus","authors":"A. Dinarvand, M. Hashemi, Rasool Dinarvand, Shabnam Movaseghi, Mojtaba Jafarinia","doi":"10.29252/iau.30.2.134","DOIUrl":"https://doi.org/10.29252/iau.30.2.134","url":null,"abstract":"Background : Most drugs used by pregnant women can pass through the placenta and expose embryos and developing embryos to teratogenic effects. Due to the increased cellular damage of Bax gene expression, the effect of cholorodiazepoxide use during pregnancy on Bax gene expression in the hippocampus of neonatal rats was investigated. Materials and methods : After confirmation of pregnancy in Wistar female rats, intraperitoneal injection of chlordiazepoxide was administered at a dose of 10 mg/ kg daily for 21 days in the experimental group. Two weeks after birth, the infant's brain was removed from the skull. After isolating the CA1 region of the neonatal hippocampus, the expression of the Bax proapoptotic gene was examined and compared with the control and carrier group (saline). Results : The level of gene expression was analyzed and propoptotic Bax gene showed significant increase in experimental group compared to control group. Conclusion : The results of this study indicated that the administration of chlorodiazepoxide during pregnancy can cause neuronal damage in the hippocampus of Wistar rats.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83303536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Masoodi, S. Rahimi, Golvash Tavakolian, S. A. Badi, Shahrbanoo Keshavarz Azizi Raftar, S. Siadat
Infantile colic is a common condition among infants in which inability of caregivers to console their infants can lead to shaken baby syndrome, child abuse, depression and etc. Therefore it can lay a great economic burden in healthcare and social welfare systems. Even though it is highly prevalent, little agreement has been reached on the definition, pathogenesis or the optimal management strategy for infantile colic. In the past decade major leaps in understanding infantile colic has been taken by researchers. Although our understanding is greater than before, but novel treatments has yet to be introduced. The current review aims to delineate the Rome IV criteria definition, which is the latest published diagnostic criteria. Moreover, gastrointestinal, neurogenic, microbial and psychosocial factors that might contribute to the pathophysiology of infant colic are explored. Also, clinical assessment of infant with suspected colic is reviewed. Important aspect of the management of infant colic is further highlighted by this review. Management strategies, including dietary, behavioral, pharmacological and alternative interventions are also discussed.
{"title":"Infantile colic: from basics to treatment","authors":"H. Masoodi, S. Rahimi, Golvash Tavakolian, S. A. Badi, Shahrbanoo Keshavarz Azizi Raftar, S. Siadat","doi":"10.29252/iau.30.1.1","DOIUrl":"https://doi.org/10.29252/iau.30.1.1","url":null,"abstract":"Infantile colic is a common condition among infants in which inability of caregivers to console their infants can lead to shaken baby syndrome, child abuse, depression and etc. Therefore it can lay a great economic burden in healthcare and social welfare systems. Even though it is highly prevalent, little agreement has been reached on the definition, pathogenesis or the optimal management strategy for infantile colic. In the past decade major leaps in understanding infantile colic has been taken by researchers. Although our understanding is greater than before, but novel treatments has yet to be introduced. The current review aims to delineate the Rome IV criteria definition, which is the latest published diagnostic criteria. Moreover, gastrointestinal, neurogenic, microbial and psychosocial factors that might contribute to the pathophysiology of infant colic are explored. Also, clinical assessment of infant with suspected colic is reviewed. Important aspect of the management of infant colic is further highlighted by this review. Management strategies, including dietary, behavioral, pharmacological and alternative interventions are also discussed.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"504 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77455397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Non-alcoholic fatty liver disease is one of the most important chronic liver disorders worldwide. Adipokines are polypeptide hormones that participate in the pathogenesis of non-alcoholic fatty liver disease. Adiponectin is an adipokine that stimulates fatty acid oxidation in muscle and plays a role in the development of insulin sensitivity. This study aimed to investigate the association between plasma adiponectin and nonalcoholic fatty liver disease. Materials and methods: This case and control study was conducted on 80 non-alcoholic fatty liver disease patients and 80 participants as control group. Plasma levels of adiponectin and insulin were measured by ELISA kit (Mercodia Company, Sweden) and other variables were determined by standard methods. Statistical analysis was performed using SPSS software, version 19. Results: Plasma adiponectin was significantly lower in non-alcoholic fatty liver disease patients than control (p-value<0.001). Whereas, subjects with non-alcoholic fatty liver disease had significantly higher levels of body mass index, serum transaminases, serum triglycerides, systolic blood pressure, serum insulin and insulin resistance compared to control group (p<0.01). An inverse correlation was observed between adiponectin with serum transaminases, triglycerides and insulin resistance (P<0.05), while it did not correlate with lipoproteins. Conclusion: The results indicate that adiponectin levels are reduced in individuals with non-alcoholic fatty liver disease. Adiponectin levels are inversely correlated with liver transaminases and insulin resistance.
{"title":"Association of adiponectin level with biochemical variables and insulin resistance in patients with non-alcoholic fatty liver disease in an Iranian population","authors":"مجتبی رحمانی, معصومه نژادعلی, جواد رستگارمقدم","doi":"10.29252/iau.29.4.329","DOIUrl":"https://doi.org/10.29252/iau.29.4.329","url":null,"abstract":"Background: Non-alcoholic fatty liver disease is one of the most important chronic liver disorders worldwide. Adipokines are polypeptide hormones that participate in the pathogenesis of non-alcoholic fatty liver disease. Adiponectin is an adipokine that stimulates fatty acid oxidation in muscle and plays a role in the development of insulin sensitivity. This study aimed to investigate the association between plasma adiponectin and nonalcoholic fatty liver disease. Materials and methods: This case and control study was conducted on 80 non-alcoholic fatty liver disease patients and 80 participants as control group. Plasma levels of adiponectin and insulin were measured by ELISA kit (Mercodia Company, Sweden) and other variables were determined by standard methods. Statistical analysis was performed using SPSS software, version 19. Results: Plasma adiponectin was significantly lower in non-alcoholic fatty liver disease patients than control (p-value<0.001). Whereas, subjects with non-alcoholic fatty liver disease had significantly higher levels of body mass index, serum transaminases, serum triglycerides, systolic blood pressure, serum insulin and insulin resistance compared to control group (p<0.01). An inverse correlation was observed between adiponectin with serum transaminases, triglycerides and insulin resistance (P<0.05), while it did not correlate with lipoproteins. Conclusion: The results indicate that adiponectin levels are reduced in individuals with non-alcoholic fatty liver disease. Adiponectin levels are inversely correlated with liver transaminases and insulin resistance.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77909086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Shivaee, Shahla Shahbazi, A. Gholami, Parham Kianoush Pour, F. Jazi
Background: Listeriosis can be fatal for vulnerable groups of society. The disease has been widespread in recent years due to the large consumption of dairy and meat products. There is little information about the susceptibility of antibiotics and the pattern of Listeria monostigenesis gene resistance in Iranian society. Accordingly, the present study was conducted to investigate the antibiotic susceptibility and genetic resistance pattern of Listeria monosteogenesis strains isolated from different clinical and environmental sources. Materials and methods: In this study, 55 isolates were tested for antibiotic susceptibility by disk diffusion in agar and genetic pattern by polymerase chain reaction (PCR). Results: 91% and 83% of the strains were resistant to streptomycin and Trimethoprim/sulfamethoxazole respectively. The result of PCR of antibiotic resistance genes showed that the prevalence of ermA, ermB, strA, tetA, tetS and ermC genes in isolates of Listeria monocytogenes was 50.90% (28/55), 21.81% (12/55), 89.9% (49/55), 0% (0/55), 21.81% (12/55) and 0% (0/55), respectively. Conclusion: Due to the presence of 1/2a and 1/2c serotypes in isolated isolates and the presence of marker virulence genes in these strains, these isolates have potential for biological risks and listeriosis disease. Existence of this genetic pattern and resistance pattern can be partly due to the use of antibiotics during the production of dairy products. Regarding results of this study, the manner and rate of using animal antibiotics can be managed.
{"title":"Investigating the prevalence of resistance genes in Listeria monocytogenes isolated from food and clinical samples","authors":"A. Shivaee, Shahla Shahbazi, A. Gholami, Parham Kianoush Pour, F. Jazi","doi":"10.29252/iau.29.4.322","DOIUrl":"https://doi.org/10.29252/iau.29.4.322","url":null,"abstract":"Background: Listeriosis can be fatal for vulnerable groups of society. The disease has been widespread in recent years due to the large consumption of dairy and meat products. There is little information about the susceptibility of antibiotics and the pattern of Listeria monostigenesis gene resistance in Iranian society. Accordingly, the present study was conducted to investigate the antibiotic susceptibility and genetic resistance pattern of Listeria monosteogenesis strains isolated from different clinical and environmental sources. Materials and methods: In this study, 55 isolates were tested for antibiotic susceptibility by disk diffusion in agar and genetic pattern by polymerase chain reaction (PCR). Results: 91% and 83% of the strains were resistant to streptomycin and Trimethoprim/sulfamethoxazole respectively. The result of PCR of antibiotic resistance genes showed that the prevalence of ermA, ermB, strA, tetA, tetS and ermC genes in isolates of Listeria monocytogenes was 50.90% (28/55), 21.81% (12/55), 89.9% (49/55), 0% (0/55), 21.81% (12/55) and 0% (0/55), respectively. Conclusion: Due to the presence of 1/2a and 1/2c serotypes in isolated isolates and the presence of marker virulence genes in these strains, these isolates have potential for biological risks and listeriosis disease. Existence of this genetic pattern and resistance pattern can be partly due to the use of antibiotics during the production of dairy products. Regarding results of this study, the manner and rate of using animal antibiotics can be managed.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"94 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91446398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abolfazl Nasirzadeh Vanhari, H. Sazgar, in Biology
Background: Positive effects of medicinal herbs on diabetes have been demonstrated in previous studies. The aim of the present study was to evaluate the effect of charantin on the treatment of diabetes and increase the expression of the Ngn3 gene in diabetes, which is a key gene for transcription of the pancreas. Materials and methods: In this experimental study, 42 adult male Wistar rats were randomly divided into 7 healthy control groups, diabetic control, control group, 150 control, and three groups receiving effective doses of charantin (50-100-200 mg/ Per kilogram). All groups except diabetic control group and control group with streptozotocin chemical injected intraperitoneally. Afterwards, they received a cartilage by gavage for four weeks (three times a week). Results: The lowest expression was observed in the diabetic group. Among those who received different doses of charantine, the highest expression was among group with dose of 100 mg/kg, and the lowest expression in 50/kg group. Conclusion: It can be concluded that increased expression of the Ngn3 gene was not dose-dependent. After the development of diabetes, the patient's severity declined sharply, and after the use of the charantin and metformin drug, this reduction was somewhat offset.
背景:草药对糖尿病的积极作用已经在以往的研究中得到证实。本研究的目的是评估charantin对糖尿病的治疗作用，并增加糖尿病中Ngn3基因的表达，该基因是胰腺转录的关键基因。材料与方法:将42只成年雄性Wistar大鼠随机分为7个健康对照组、糖尿病对照组、对照组、150只对照组和3组，给予有效剂量(50-100-200 mg/ Per kg)。各组除糖尿病对照组和对照组腹腔注射链脲佐菌素外。然后给予软骨灌胃4周(每周3次)。结果:糖尿病组表达最低。不同剂量夏陵组中，100 mg/kg剂量组表达量最高，50 mg/kg剂量组表达量最低。结论:Ngn3基因表达的增加不具有剂量依赖性。在发生糖尿病后，患者的严重程度急剧下降，在使用charantin和二甲双胍药物后，这种下降在一定程度上被抵消了。
{"title":"The effect of charantin on expression of Ngn3 gene in streptozotocin-induced diabetic rats","authors":"Abolfazl Nasirzadeh Vanhari, H. Sazgar, in Biology","doi":"10.29252/iau.29.4.303","DOIUrl":"https://doi.org/10.29252/iau.29.4.303","url":null,"abstract":"Background: Positive effects of medicinal herbs on diabetes have been demonstrated in previous studies. The aim of the present study was to evaluate the effect of charantin on the treatment of diabetes and increase the expression of the Ngn3 gene in diabetes, which is a key gene for transcription of the pancreas. Materials and methods: In this experimental study, 42 adult male Wistar rats were randomly divided into 7 healthy control groups, diabetic control, control group, 150 control, and three groups receiving effective doses of charantin (50-100-200 mg/ Per kilogram). All groups except diabetic control group and control group with streptozotocin chemical injected intraperitoneally. Afterwards, they received a cartilage by gavage for four weeks (three times a week). Results: The lowest expression was observed in the diabetic group. Among those who received different doses of charantine, the highest expression was among group with dose of 100 mg/kg, and the lowest expression in 50/kg group. Conclusion: It can be concluded that increased expression of the Ngn3 gene was not dose-dependent. After the development of diabetes, the patient's severity declined sharply, and after the use of the charantin and metformin drug, this reduction was somewhat offset.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"106 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73545708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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