Microvascular research最新文献_第8页

A cost-effective vessel-on-a-chip for high shear stress applications in vascular biology 一种具有成本效益的血管芯片，用于血管生物学中的高剪切应力应用

IF 2.9 4区医学 Q2 PERIPHERAL VASCULAR DISEASE

Microvascular research

Pub Date : 2025-05-03 DOI: 10.1016/j.mvr.2025.104814

Clarissa Becher , Martin Frauenlob , Florian Selinger , Peter Ertl , Marie-José Goumans , Gonzalo Sanchez-Duffhues

The vascular endothelium is constantly subjected to hemodynamic forces, including tangential shear stress, which are crucial for maintaining vascular homeostasis. Pathological shear stress levels, such as those observed in pulmonary arterial hypertension (PAH) or atherosclerosis, disrupt this balance, driving vascular remodeling and endothelial dysfunction. Current microfluidic platforms for studying these conditions are limited by high costs, excessive reagent requirements, and non-physiological channel geometries. Here we introduce a novel microfluidic chip system, a Nylon Vessel-on-a-Chip (NVoC) which represents a cost-effective and straightforward fabrication platform that eliminates the need for specialized equipment and enables a physiologically relevant round channel geometry. The NVoC was fabricated using Polydimethylsiloxane (PDMS) and nylon threads, with surface activation achieved through polydopamine and collagen-I coating, enabling robust endothelial cell (EC) attachment and long-term culture. Immortalized endothelial colony-forming cells (iECFCs) and human umbilical vein EC (HUVECs) were used to optimize and validate the platform, demonstrating its compatibility with high shear stress conditions (up to 90 dyne/cm²) and various molecular biology techniques, including RT-qPCR, Western blotting, and immunofluorescent staining. With fabrication costs six times lower than commercial alternatives and overall experimental costs reduced threefold, the NVoC offers the ability to expose endothelial cells to physiological and pathological shear stress levels in a reproducible, accessible, and scalable manner. Its versatility and affordability make it a valuable tool for investigating shear stress-related mechanisms in microvascular diseases, particularly PAH, with potential applications in drug discovery and translational research.

血管内皮不断受到血流动力学力的影响，包括切向剪切应力，这对维持血管稳态至关重要。病理性剪切应力水平，如在肺动脉高压（PAH）或动脉粥样硬化中观察到的水平，破坏了这种平衡，驱动血管重塑和内皮功能障碍。目前用于研究这些条件的微流控平台受到高成本、过多试剂需求和非生理通道几何形状的限制。在这里，我们介绍了一种新的微流控芯片系统，尼龙容器芯片（NVoC），它代表了一种具有成本效益和直接的制造平台，消除了对专门设备的需求，并实现了生理相关的圆形通道几何形状。NVoC由聚二甲基硅氧烷（PDMS）和尼龙线制成，通过聚多巴胺和胶原- i涂层实现表面激活，使内皮细胞（EC）附着和长期培养稳定。利用永生化内皮细胞集落形成细胞（iecfc）和人脐静脉细胞集落形成细胞（HUVECs）对平台进行优化和验证，证明其与高剪切应力条件（高达90 dyne/cm2）和各种分子生物学技术（包括RT-qPCR， Western blotting和免疫荧光染色）的兼容性。NVoC的制造成本比商业替代品低6倍，总体实验成本降低了3倍，能够以可复制、可获取和可扩展的方式将内皮细胞暴露在生理和病理剪切应力水平下。它的多功能性和可负担性使其成为研究微血管疾病（特别是多环芳烃）中剪切应力相关机制的宝贵工具，在药物发现和转化研究中具有潜在的应用前景。

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引用次数: 0

Proteomics suggests the role of Cxcl12 secreted by hucMSCs in the treatment of lipopolysaccharide-acute lung injury 蛋白质组学提示humscs分泌的Cxcl12在脂多糖急性肺损伤治疗中的作用

IF 2.9 4区医学 Q2 PERIPHERAL VASCULAR DISEASE

Microvascular research

Pub Date : 2025-04-30 DOI: 10.1016/j.mvr.2025.104815

Jinfeng Cui , Liqing Luo , Hongmei Geng , Yunxiu Gao , Yuanyuan Chen , Qilin Yu , Xiao Huang , Xiaozhi Wang , Ting Sun

Acute respiratory distress syndrome (ARDS) is a clinical syndrome characterized by a high mortality rate, and its treatment is relatively straightforward. The application of human umbilical cord mesenchymal stem cells (hucMSCs) for the treatment of ARDS has emerged as a novel therapeutic approach and has been the subject of extensive research. In this study, a mouse model of acute lung injury (ALI) was established, and hucMSCs were administered via tail vein injection to investigate the pathogenesis of ARDS and the protein alterations following hucMSC treatment. Data-independent acquisition (DIA) was employed for the proteomic analysis of lung tissue, which included the identification of differentially expressed proteins (DEPs) and their associated pathways. The relevant DEPs identified in the lung tissues of the three groups of mice included Arid5a, Mrpl4, Cxcl12, and Rnf121 (P <0.05). Silencing the expression of Cxcl12 in hucMSCs could significantly inhibit the therapeutic effect of hucMSCs in reducing the permeability of lung tissue and endothelial cells (P < 0.05). Additionally, the signaling pathways associated with the relevant DEPs were analyzed. The DEPs and the enriched pathways discussed herein provide valuable insights into the pathogenesis of ARDS and the potential applications of hucMSCs.

急性呼吸窘迫综合征（Acute respiratory distress syndrome， ARDS）是一种死亡率高的临床综合征，治疗方法相对简单。应用人脐带间充质干细胞（hucMSCs）治疗ARDS已成为一种新的治疗方法，并已成为广泛研究的主题。本研究建立小鼠急性肺损伤（ALI）模型，通过尾静脉注射给药humscs，探讨ARDS的发病机制及humscs治疗后蛋白的改变。采用数据独立采集（DIA）对肺组织进行蛋白质组学分析，包括鉴定差异表达蛋白（DEPs）及其相关途径。在三组小鼠肺组织中发现的相关DEPs包括Arid5a、Mrpl4、Cxcl12和Rnf121 （P <0.05）。沉默Cxcl12在hucMSCs中的表达可显著抑制hucMSCs降低肺组织和内皮细胞通透性的治疗作用(P <；0.05)。此外，我们还分析了与相关dep相关的信号通路。本文讨论的dep和富集通路为ARDS的发病机制和hucMSCs的潜在应用提供了有价值的见解。

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引用次数: 0

Effects of veno-arterial extracorporeal membrane oxygenation on skeletal muscle function and interstitial PO2 in contracting muscle of normal rats 静脉-动脉体外膜氧合对正常大鼠骨骼肌功能及收缩肌间质PO2的影响

IF 2.9 4区医学 Q2 PERIPHERAL VASCULAR DISEASE

Microvascular research

Pub Date : 2025-04-23 DOI: 10.1016/j.mvr.2025.104813

Kazuki Hotta , Yutaka Fujii , Naoki Hitosugi , Ren Takamizawa , Tatsuro Inoue , Hajime Tamiya , Atsuhiro Tsubaki

Background

This study aimed to clarify the effects of veno-arterial extracorporeal membrane oxygenation (VA-ECMO) on skeletal muscle oxygen pressure and function in rats.

Methods

Male Sprague-Dawley rats (2–3 months old, n = 17) were randomized into control and VA-ECMO groups. All animals were anesthetized and mechanically ventilated. The VA-ECMO circuit was established by cannulating the right jugular vein and left carotid artery. Interstitial PO₂ in the tibialis anterior (TA) muscle was measured using a phosphorescence quenching technique during electrically induced muscle contractions. Muscle tension was analyzed to evaluate the rate of force development (RFD) and relaxation rate.

Results

Compared to controls, arterial oxygen pressure (PaO₂) was significantly higher, while hemoglobin levels were significantly lower in the VA-ECMO group (both p < 0.01). Interstitial PO₂ was significantly reduced at rest and during contractions in the VA-ECMO group (both p < 0.01). Muscle relaxation was delayed, and peak tension was lower in the VA-ECMO group compared to controls (both p < 0.01).

Conclusions

VA-ECMO impairs skeletal muscle function and reduces interstitial PO2 in contracting muscles, effects that appear independent of hyperoxemia. These findings provide insight into the microcirculatory and functional consequences of VA-ECMO on skeletal muscle.

本研究旨在阐明静脉-动脉体外膜氧合（VA-ECMO）对大鼠骨骼肌氧压和功能的影响。方法选取2 ~ 3月龄的雄性sd大鼠17只，随机分为对照组和VA-ECMO组。所有动物均麻醉并机械通气。通过右颈静脉和左颈动脉插管建立VA-ECMO回路。在电诱导肌肉收缩时，采用磷光猝灭技术测量胫骨前肌间质PO2。分析肌肉张力，评估力发展率（RFD）和松弛率。结果与对照组相比，VA-ECMO组动脉氧压（PaO2）显著升高，血红蛋白水平显著降低(p <；0.01)。VA-ECMO组间质PO2在静息和收缩时均显著降低(p <；0.01)。与对照组相比，VA-ECMO组肌肉松弛延迟，峰值张力较低(p <；0.01)。结论sva - ecmo损害骨骼肌功能，降低收缩肌间质PO2，其作用与高氧血症无关。这些发现为VA-ECMO对骨骼肌的微循环和功能影响提供了见解。

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引用次数: 0

Glial and blood-brain barrier cell-derived exosomes: Implications in stroke 神经胶质和血脑屏障细胞衍生的外泌体：对中风的影响

IF 2.9 4区医学 Q2 PERIPHERAL VASCULAR DISEASE

Microvascular research

Pub Date : 2025-04-15 DOI: 10.1016/j.mvr.2025.104812

Khiany Mathias , Richard Simon Machado , Taise Petronilho , Victor Augusto Rodrigues Sulzbacher , Victoria Linden de Rezende , Josiane Somariva Prophiro , Fabricia Petronilho

Exosomes are small extracellular vesicles released by cells that play a pivotal role in intercellular communication, significantly influencing both the pathophysiology and potential treatment of ischemic stroke (IS). This review examines exosomes derived from key brain cell types, including microglia, astrocytes, oligodendrocytes, oligodendrocyte precursor cells (NG2+ cells), endothelial cells, and pericytes, emphasizing their molecular cargo and functional impact in IS. Microglia-derived exosomes regulate neuroinflammation, with M2-type exosomes exhibiting neuroprotective effects, while astrocyte-derived exosomes modulate pathways involved in pyroptosis and autophagy, influencing neuronal survival. Oligodendrocyte and NG2+ cell-derived exosomes contribute to remyelination, axonal growth, and inflammatory modulation. Endothelial and pericyte-derived exosomes play critical roles in BBB integrity, neurovascular remodeling, and drug transport across the BBB. This synthesis highlights recent advances in understanding how exosome-mediated communication impacts IS recovery and explores their translational potential for biomarker development and targeted therapies. By manipulating exosomal composition and delivery mechanisms, novel therapeutic strategies may emerge, offering hope for improved IS treatment outcomes.

外泌体是细胞释放的小细胞外囊泡，在细胞间通讯中起关键作用，显著影响缺血性卒中（IS）的病理生理和潜在治疗。本文综述了来自主要脑细胞类型的外泌体，包括小胶质细胞、星形胶质细胞、少突胶质细胞、少突胶质细胞前体细胞（NG2+细胞）、内皮细胞和周细胞，强调了它们的分子货物和在IS中的功能影响。小胶质细胞来源的外泌体调节神经炎症，其中m2型外泌体具有神经保护作用，而星形胶质细胞来源的外泌体调节参与焦亡和自噬的途径，影响神经元存活。少突胶质细胞和NG2+细胞衍生的外泌体有助于髓鞘再生、轴突生长和炎症调节。内皮细胞和周细胞来源的外泌体在血脑屏障完整性、神经血管重塑和药物跨血脑屏障运输中发挥关键作用。本综述强调了外泌体介导的通讯如何影响IS恢复的最新进展，并探索了它们在生物标志物开发和靶向治疗方面的转化潜力。通过操纵外泌体组成和传递机制，可能会出现新的治疗策略，为改善IS治疗结果提供希望。

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引用次数: 0

The influence of cuff location on the oxygenation and reperfusion of the foot during ischemic preconditioning: A reliability study 在缺血预处理中袖带位置对足部氧合和再灌注的影响：一项可靠性研究

IF 2.9 4区医学 Q2 PERIPHERAL VASCULAR DISEASE

Microvascular research

Pub Date : 2025-04-15 DOI: 10.1016/j.mvr.2025.104811

Chloe French , Dan Robbins , Marie Gernigon , Dan Gordon

Ischemic preconditioning (IPC) involves the application of occlusion cycles, typically prior to exercise. IPC is commonly applied at the arm or thigh for improving exercise performance, which can be combined with near-infrared spectroscopy (NIRS) to assess the microcirculation and tissue oxygenation. Despite the use of NIRS during IPC, few studies have investigated the reliability of NIRS during lower limb IPC with no relevant publications investigating IPC at the ankle. Therefore, the purpose of this study was to investigate the intra-session reliability in the NIRS measurements during repeated IPC at the thigh, ankle and arm. Eighteen participants volunteered. IPC was applied at the thigh (220 mmHg), ankle (individualized arterial occlusion pressure: 212 ± 24 mmHg) and arm (220 mmHg) in a randomized order involving 3 repeated cycles of 5-min occlusion and reperfusion, within a session. NIRS recorded tissue oxygen saturation (SO₂), oxygenated (O₂Hb) and deoxygenated hemoglobin (HHb) at the abductor hallucis muscle. Reliability was assessed using intraclass correlation coefficients. For all NIRS measurements assessed, there was excellent reliability (All ICC > 0.94) for the average, minimum and maximum values. The results indicate that IPC can successfully be applied at the ankle, offering reliable measures between three repeated occlusions within a session.

缺血预处理（IPC）涉及闭塞循环的应用，通常在运动之前。IPC通常应用于手臂或大腿，以提高运动表现，可与近红外光谱（NIRS）结合评估微循环和组织氧合。尽管在IPC期间使用了近红外光谱，但很少有研究调查近红外光谱在下肢IPC期间的可靠性，没有相关的出版物调查踝关节的IPC。因此，本研究的目的是研究在大腿、脚踝和手臂重复IPC期间NIRS测量的会话内可靠性。18名参与者自愿参加。IPC应用于大腿（220 mmHg）、脚踝（个体化动脉闭塞压：212±24 mmHg）和手臂（220 mmHg），随机顺序为3个重复周期，在一个疗程内进行5分钟的闭塞和再灌注。近红外光谱记录了幻觉外展肌组织氧饱和度（SO2）、氧合血红蛋白（O2Hb）和脱氧血红蛋白（hbb）。用类内相关系数评估信度。对于所有评估的近红外测量，都有极好的可靠性(all ICC >；0.94)表示平均值、最小值和最大值。结果表明，IPC可以成功地应用于踝关节，在一个疗程内提供三次重复闭塞之间的可靠测量。

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引用次数: 0

Localized pulmonary vascular changes in a mouse model of subarachnoid hemorrhage created by combining filament perforation and blood injection 蛛网膜下腔出血小鼠模型的局部肺血管改变

IF 2.9 4区医学 Q2 PERIPHERAL VASCULAR DISEASE

Microvascular research

Pub Date : 2025-04-13 DOI: 10.1016/j.mvr.2025.104810

Ryota Tochinai , Takaya Suzuki , Kenji Tomita , Shin-ichi Sekizawa , Yoshinori Okada , Yasuyuki Taki , Masayoshi Kuwahara , Tatsushi Mutoh

Introduction

Subarachnoid hemorrhage (SAH) results in neurogenic pulmonary edema (NPE), a condition with a high mortality rate arising from increased hydrostatic pressure and vascular permeability. Two possible mechanisms of NPE are increased hydrostatic pressure and increased vascular permeability, and it is possible that increased permeability of capillaries in the lungs may contribute to the exacerbation of NPE. Recent research has highlighted the importance of the glycocalyx, a gel-like layer that lines blood vessels, in regulating vascular permeability in various diseases. However, its role in NPE after SAH has not been previously explored. This study investigated the involvement of the glycocalyx in the development of NPE by developing a mouse model of SAH.

Methods

The SAH model was developed by combining internal carotid artery (ICA) perforation and blood infusion into the cisterna magna of mice. The histological structure of the lungs was confirmed using micro-CT, histopathological examination, and scanning electron microscopy.

Results

Despite no obvious micro-CT findings indicating pulmonary edema, histopathological changes in hematoxylin and eosin-stained lung were detected. Scanning electron microscopy revealed glycocalyx exfoliation within the pulmonary microvascular wall. A trend toward higher plasma syndecan-1 levels was also observed.

Conclusion

The combination of ICA perforation and blood infusion into the cisterna magna can produce pulmonary findings in mice that mimic NPE after SAH. The results also suggest that glycocalyx loss is involved in the development of NPE after SAH.

蛛网膜下腔出血（SAH）导致神经源性肺水肿（NPE），这是一种由静水压力和血管渗透性增加引起的高死亡率的疾病。NPE的两种可能机制是静水压力增加和血管通透性增加，肺部毛细血管通透性增加可能导致NPE的加重。最近的研究强调了糖萼（排列在血管上的凝胶状层）在调节各种疾病的血管通透性方面的重要性。然而，其在SAH后NPE中的作用尚未被探讨。本研究通过建立小鼠SAH模型来探讨糖萼在NPE发生中的作用。方法采用颈动脉穿孔和大池输血相结合的方法建立小鼠SAH模型。显微ct、组织病理学检查及扫描电镜检查证实肺组织结构。结果微ct未见明显肺水肿，但苏木精染色及伊红染色肺组织病理改变。扫描电镜显示肺微血管壁内糖萼脱落。血浆中syndecan-1水平也有升高的趋势。结论在SAH后，ICA穿孔和大池输注联合可引起小鼠模拟NPE的肺部表现。结果还表明，糖萼丢失参与了SAH后NPE的发展。

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引用次数: 0

Increase in endothelial microparticles is negatively correlated with decrease in renal microperfusion in septic rats 内皮微粒的增加与脓毒症大鼠肾微灌注的减少呈负相关。

IF 2.9 4区医学 Q2 PERIPHERAL VASCULAR DISEASE

Microvascular research

Pub Date : 2025-04-03 DOI: 10.1016/j.mvr.2025.104809

Xinjie Guo , Jingfeng Liu , Meili Duan

Introduction

Endothelial dysfunction is an important pathophysiological mechanism of septic acute kidney injury, and endothelial microparticles (EMPs) can directly reflect the endothelial damage. However, the relationship between EMPs and renal microperfusion remains unclear. In this study, contrast-enhanced ultrasound (CEUS) imaging and side-stream dark field imaging were used to evaluate the renal microcirculatory perfusion in septic rats.

Methods

A cecal ligation and puncture model was established for inducing septic kidney injury in Sprague-Dawley rats. Later, the changes in mean arterial pressure (MAP), lactate level, renal artery blood flow (RBF) and mean renal artery velocity were measured. Flow cytometry was conducted to measure EMPs, CEUS imaging was performed to evaluate cortical and medullary perfusion enhancement, and side-stream dark-field imaging was carried out to detect the perfused small vessel density (PVD) and microvascular flow index of the renal cortex.

Results

In the sepsis group, EMPs and lactate levels increased at 12 h, macrohemodynamics (MAP and RBF) did not change, and the mean artery velocity (547.76 ± 28.40 mm/s) increased compared with the sham group (421.78 ± 34.58 mm/s). Meanwhile, cortical peak echointensity (PE), medullary PE, PVD, and microvascular flow index (MFI) decreased at 12 h. The decreases in pulsatility index (PI) and resistance index (RI) suggested the damage of vascular appearance. The pathological results revealed erythrocyte stasis in the capillaries. At 24 h, macrodynamics decreased compared with that at 12 h. The EMPs and lactate levels reached a peak at 24 h. Glomerular vascular endothelium was locally thickened. Moreover, EMPs were negatively correlated with the decreased renal microcirculatory perfusion.

Conclusions

This study shows that endothelial microparticles (EMPs) are closely associated with renal microcirculatory dysfunction in septic acute kidney injury (S-AKI). CEUS can sensitively reflect changes in renal microperfusion, providing earlier indications of kidney injury compared to macrocirculatory changes, and holds potential for early diagnosis of S-AKI.

内皮功能障碍是脓毒性急性肾损伤的重要病理生理机制，内皮微粒（Endothelial microparticles, EMPs）可直接反映内皮损伤情况。然而，EMPs与肾微灌注之间的关系尚不清楚。本研究采用超声造影（CEUS）和侧流暗场成像技术评价脓毒症大鼠肾脏微循环灌注。方法：建立sd - dawley大鼠败血性肾损伤盲肠结扎穿刺模型。测定各组平均动脉压（MAP）、乳酸水平、肾动脉血流量（RBF）和平均肾动脉流速的变化。流式细胞术测量emp，超声造影评估皮质和髓质灌注增强，侧流暗场成像检测肾皮质灌注小血管密度（PVD）和微血管流动指数。结果：脓毒症组EMPs和乳酸水平在12 h时升高，大血流动力学（MAP和RBF）无变化，平均动脉流速（547.76 ± 28.40 mm/s）高于假手术组（421.78 ± 34.58 mm/s）。同时，皮质峰值回波强度（PE）、髓质PE、PVD和微血管血流指数（MFI）在12 h时下降。搏动指数（PI）和阻力指数（RI）下降提示血管外观损伤。病理结果显示毛细血管内红细胞淤积。与12 h相比，24 h时宏观动力学降低。emp和乳酸水平在24 h时达到峰值。肾小球血管内皮局部增厚。EMPs与肾微循环灌注降低呈负相关。结论：本研究表明，在脓毒性急性肾损伤（S-AKI）中，内皮微粒（EMPs）与肾微循环功能障碍密切相关。超声造影能敏感地反映肾脏微灌注的变化，与大循环变化相比，能提供更早的肾损伤指示，具有早期诊断S-AKI的潜力。

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引用次数: 0

KATP channel inhibition-induced hyporemia in skeletal muscle: No evidence for pre-capillary sphincter action KATP 通道抑制诱导的骨骼肌低血流量：没有证据表明毛细血管前括约肌起作用。

IF 2.9 4区医学 Q2 PERIPHERAL VASCULAR DISEASE

Microvascular research

Pub Date : 2025-03-29 DOI: 10.1016/j.mvr.2025.104808

Kiana M. Schulze , Daniel M. Hirai , Trenton D. Colburn , Jesse C. Craig , Timothy I. Musch , David C. Poole

Introduction

Whether pre-capillary sphincters are present and regulate red blood cell (RBC) flux at the individual capillary level, especially in skeletal muscle, is controversial. Recently, blockade of K_ATP channels using the sulphonylurea glibenclamide (GLI) was demonstrated to reduce muscle blood flow and lower vascular conductance. The present investigation tested the hypothesis that, if pre-capillary sphincters were involved in GLI-induced blood flow reductions, a defined luminal narrowing would be evident in the proximate region of the capillaries.

Methods

Videomicroscopy of the spinotrapezius capillary bed was performed under control (Krebs-Henseleit) and GLI (200 μM in Krebs-Henseleit) superfusion. Capillary RBC flux was measured within individual capillaries and their luminal diameter was measured using a calibrated digital ruler at the branch-point and subsequently downstream.

Results

GLI reduced capillary RBC flux by 31% (p = 0.004). Despite the presence of a reduced RBC flux, no detectable reduction or, indeed, any change in capillary luminal diameter was present at any measurement site. The average diameter at the branching point was 4.9 ± 0.3 μm, and at 5, 10, 20 and 50 μm downstream, the average diameters were 4.8 ± 0.4, 4.8 ± 0.5, 5.0 ± 0.7, and 5.2 ± 0.4 μm, respectively and were unchanged by GLI (all P > 0.05).

Conclusions

Accordingly, the absence of any evidence for capillary luminal narrowing or constriction in these data support that the GLI-induced reductions in capillary RBC flux and muscle blood flow occur via upstream effects within the arteriolar bed. Decreases in skeletal muscle microcirculatory RBC flux with this K_ATP channel blocker were not regulated by any detectable capillary structural alterations.

导言：毛细血管前括约肌是否存在并在个体毛细血管水平上调节红细胞（RBC）通量，特别是在骨骼肌中，是有争议的。最近，使用磺脲类格列本脲（GLI）阻断KATP通道被证明可以减少肌肉血流量和降低血管传导。目前的研究验证了这样一个假设，即如果毛细血管前括约肌参与了胶质细胞诱导的血流减少，那么毛细血管近端区域明显存在明确的管腔狭窄。方法：在对照（Krebs-Henseleit）和GLI（200 μM in Krebs-Henseleit）灌流下对斜方肌毛细血管床进行视频显微镜观察。在单个毛细血管内测量毛细血管红细胞通量，在分支点和随后的下游使用校准的数字尺测量其管径。结果：GLI使毛细血管红细胞通量降低31% % （p = 0.004）。尽管存在红细胞通量减少，但在任何测量部位均未发现毛细血管管腔直径的减少或变化。在分支点的平均直径为4.9 ±0.3  μm,在5、10、20、50 μm下游,平均直径是4.8 ± 0.4,4.8 ± 0.5,5.0 ± 0.7,和5.2 ±0.4  μm,分别和持平GLI(所有P > 0.05)。结论：因此，在这些数据中没有任何毛细血管管腔狭窄或收缩的证据，这支持了glii诱导的毛细血管红细胞通量和肌肉血流量的减少是通过小动脉床内的上游效应发生的。这种KATP通道阻滞剂对骨骼肌微循环红细胞通量的降低不受任何可检测到的毛细血管结构改变的调节。

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引用次数: 0

Corrigendum to “Morphometrics of polypoidal choroidal vasculopathy lesions and choroidal vascular associated with treatment response using swept-source optical coherence tomography angiography” [Microvasc. Res. 157 (2025) 104759] 多形性脉络膜血管病病变的形态计量学以及使用扫源光学相干断层血管造影与治疗反应相关的脉络膜血管》[Microvasc. Res. 157 (2025) 104759]的更正。

IF 2.9 4区医学 Q2 PERIPHERAL VASCULAR DISEASE

Microvascular research

Pub Date : 2025-03-22 DOI: 10.1016/j.mvr.2025.104807

Yue Zhang , Jianing Wang , Zhaoxia Zheng , Shuang Song , Xiaoya Gu , Xiaobing Yu

引用次数: 0

Lymphatics in the chick embryo chorioallantoic membrane 鸡胚绒毛尿囊膜中的淋巴管

IF 2.9 4区医学 Q2 PERIPHERAL VASCULAR DISEASE

Microvascular research

Pub Date : 2025-03-21 DOI: 10.1016/j.mvr.2025.104806

Domenico Ribatti

The chick embryo chorioallantoic membrane (CAM) has been used as an experimental in vivo model to study angiogenesis and anti-angiogenesis. Moreover, due to the lack of a fully developed immunocompetent system, the CAM is suitable to study various aspects of tumor angiogenesis and metastatic potential. In this article, we emphasize the important role of the CAM also in the study of lymphangiogenesis and tumor lymphangiogenesis in vivo. This experimental model is more advantageous than other assays because it is a relatively simple, quick, and low-cost. Finally, it does not require administrative procedures to obtain ethics committee approval for animal experimentation.

以鸡胚绒毛尿囊膜（CAM）作为体内血管生成和抗血管生成的实验模型。此外，由于缺乏成熟的免疫系统，CAM适合研究肿瘤血管生成和转移潜能的各个方面。在本文中，我们强调了CAM在体内淋巴管生成和肿瘤淋巴管生成研究中的重要作用。该实验模型比其他分析方法更有优势，因为它相对简单、快速和低成本。最后，它不需要行政程序来获得伦理委员会对动物实验的批准。

引用次数: 0