D. I. Astuti, I. Taufik, Dini Achnafani, Ezra Suci Priscila
Terasi or shrimp paste is an Indonesian traditional seasoning made from fermented small shrimp or krill. Different indigenous microorganism community exhibit different physiological function due to lack standard in its materials and processing. This study aimed to determine physiological profiles and microorganism community in Cirebon shrimp paste fermentation. BIOLOG TM EcoPlate was used to obtain microbial physiological function of the krill and 2-months old shrimp paste. Microorganisms were later isolated from EcoPlate substrate to determine its community structure. Average Well Color Development (AWCD) from krill was thirty times higher than shrimp paste. Interestingly, this study revealed a shift of carbon source utilization at day-28 of fermentation from amino acid and polymer to phenolic compound. In addition, AWCD index increased in accordance with increased of microorganism community complexity at day-28. Within 56 days of fermentation there was a slightly increase in water, fat, and carbohydrate content. In contrast, there was decrease in protein, ash content, and acidity level from neutral to acid, with salinity level resulted in between 16.26% to 21.42%. We conclude that there is a change of microorganism community within shrimp paste fermentation corresponding to metabolism activity which affects the product quality.
{"title":"Physiological Profiling and Microorganism Community Analysis of Cirebon Shrimp Paste Fermentation “Terasi” using BIOLOGTM EcoPlate","authors":"D. I. Astuti, I. Taufik, Dini Achnafani, Ezra Suci Priscila","doi":"10.5454/mi.12.1.3","DOIUrl":"https://doi.org/10.5454/mi.12.1.3","url":null,"abstract":"Terasi or shrimp paste is an Indonesian traditional seasoning made from fermented small shrimp or krill. Different indigenous microorganism community exhibit different physiological function due to lack standard in its materials and processing. This study aimed to determine physiological profiles and microorganism community in Cirebon shrimp paste fermentation. BIOLOG TM EcoPlate was used to obtain microbial physiological function of the krill and 2-months old shrimp paste. Microorganisms were later isolated from EcoPlate substrate to determine its community structure. Average Well Color Development (AWCD) from krill was thirty times higher than shrimp paste. Interestingly, this study revealed a shift of carbon source utilization at day-28 of fermentation from amino acid and polymer to phenolic compound. In addition, AWCD index increased in accordance with increased of microorganism community complexity at day-28. Within 56 days of fermentation there was a slightly increase in water, fat, and carbohydrate content. In contrast, there was decrease in protein, ash content, and acidity level from neutral to acid, with salinity level resulted in between 16.26% to 21.42%. We conclude that there is a change of microorganism community within shrimp paste fermentation corresponding to metabolism activity which affects the product quality.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":"12 1","pages":"3"},"PeriodicalIF":0.0,"publicationDate":"2018-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86008908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Six selected lactic acid bacteria (LAB) isolates from pickled Yellow Betung bamboo shoots were grown in de Mann Rogosa Sharpe-Broth (MRSB) media with different supplementation combination. The cell-free supernatant were evaluated for their ability to produce bacteriocin by adjusting its pH to 6.0 in order to remove organic acid effects. The bacteriocin activity was assayed by agar-well diffusion method. The inhibitory activity calculated in Activity Unit (AU in mm 2 mL -1 ) of bacteriocins. The aims of this paper is to explore the effect of different medium compositions on bacteriocin production and its inhibitory activity against pathogenic bacteria ( Listeria monocytogenes FNCC 0156, S taphylococcus aureus FNCC 0047, and E scherichia coli FNCC 0091).Supplementations of carbon and nitrogen sources induced production of bacteriocins. LAB isolates grown in media without supplementation could not produce bacteriocins. Growth of isolate D44 in the presence of 2% of glucose and 2% of yeast extract yielded the largest bacteriocin inhibitory activity levels of 3179 AU mL -1 against Listeria monocytogenes FNCC 0156, 4663 AU mL -1 against S taphylococcus aureus FNCC 0047, and 3109 AU mL -1 against E scherichia coli FNCC 0091.
从腌制黄竹笋中筛选出6株乳酸菌(LAB)，在不同添加组合的MRSB培养基中进行培养。为了去除有机酸的影响，将无细胞上清液的pH调节到6.0，以评估其产生细菌素的能力。采用琼脂孔扩散法测定菌素活性。细菌素的抑菌活性以活性单位(AU单位:mm 2 mL -1)计算。本文旨在探讨不同培养基组成对细菌素产生的影响及其对病原菌(单核增生李斯特菌FNCC 0156、金黄色葡萄球菌FNCC 0047和大肠杆菌FNCC 0091)的抑制活性。补充碳源和氮源诱导细菌素的产生。培养基中培养的乳酸菌不产生细菌素。分离物D44在2%葡萄糖和2%酵母提取物的条件下生长，对单核增生李斯特菌FNCC 0156、金黄色葡萄球菌FNCC 0047和大肠杆菌FNCC 0091的抑菌活性最高，分别为3179 AU mL -1、4663 AU mL -1和3109 AU mL -1。
{"title":"THE EFFECT OF CARBON AND NITROGEN SUPPLEMENTATION ON BACTERIOCIN PRODUCTION OF LACTIC ACID BACTERIA FROM PICKLED YELLOW BAMBOO SHOOTS (Dendrocalamus asper)","authors":"Laksmi Hartajanie, L. Lindayani, Lorentia Santoso","doi":"10.5454/MI.12.1.2","DOIUrl":"https://doi.org/10.5454/MI.12.1.2","url":null,"abstract":"Six selected lactic acid bacteria (LAB) isolates from pickled Yellow Betung bamboo shoots were grown in de Mann Rogosa Sharpe-Broth (MRSB) media with different supplementation combination. The cell-free supernatant were evaluated for their ability to produce bacteriocin by adjusting its pH to 6.0 in order to remove organic acid effects. The bacteriocin activity was assayed by agar-well diffusion method. The inhibitory activity calculated in Activity Unit (AU in mm 2 mL -1 ) of bacteriocins. The aims of this paper is to explore the effect of different medium compositions on bacteriocin production and its inhibitory activity against pathogenic bacteria ( Listeria monocytogenes FNCC 0156, S taphylococcus aureus FNCC 0047, and E scherichia coli FNCC 0091).Supplementations of carbon and nitrogen sources induced production of bacteriocins. LAB isolates grown in media without supplementation could not produce bacteriocins. Growth of isolate D44 in the presence of 2% of glucose and 2% of yeast extract yielded the largest bacteriocin inhibitory activity levels of 3179 AU mL -1 against Listeria monocytogenes FNCC 0156, 4663 AU mL -1 against S taphylococcus aureus FNCC 0047, and 3109 AU mL -1 against E scherichia coli FNCC 0091.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":"20 1","pages":"2-2"},"PeriodicalIF":0.0,"publicationDate":"2018-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84816890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Chaerun, Frideni Yushandiana Putri, Wahyudin Prawita Minwal, Z. T. Ichlas, M. Z. Mubarok
The bioleaching of an Indonesian complex copper sulfide ore was studied in shake flasks over a period of 14 days using an iron-oxidizing indigenous bacterium at room temperature (28 o C) and various pulp densities (5% and 20%). The bioleaching suspensions were periodically analyzed for Cu and Fe concentrations as well as Eh, pH and DO values. Cu bioleaching efficiencies at 5% pulp density were higher than those at 20% pulp density, which correlated with Fe concentration in solution. Over a period of 14 days, the pH of bioleaching suspension was in the range of 5 ~ 9, indicating that Cu bioleaching was greatly influenced not only by proton H + dan ferric ion but also by extracellular polymeric substances (EPS) generated by the bacterium. The current study may improve our better understanding on the bacterial action for bioleaching of complex copper sulfide ores that remains debated so far as refractory ores.
{"title":"Bacterial leaching of an Indonesian complex copper sulfide ore using an iron-oxidizing indigenous bacterium","authors":"S. Chaerun, Frideni Yushandiana Putri, Wahyudin Prawita Minwal, Z. T. Ichlas, M. Z. Mubarok","doi":"10.5454/MI.12.1.1","DOIUrl":"https://doi.org/10.5454/MI.12.1.1","url":null,"abstract":"The bioleaching of an Indonesian complex copper sulfide ore was studied in shake flasks over a period of 14 days using an iron-oxidizing indigenous bacterium at room temperature (28 o C) and various pulp densities (5% and 20%). The bioleaching suspensions were periodically analyzed for Cu and Fe concentrations as well as Eh, pH and DO values. Cu bioleaching efficiencies at 5% pulp density were higher than those at 20% pulp density, which correlated with Fe concentration in solution. Over a period of 14 days, the pH of bioleaching suspension was in the range of 5 ~ 9, indicating that Cu bioleaching was greatly influenced not only by proton H + dan ferric ion but also by extracellular polymeric substances (EPS) generated by the bacterium. The current study may improve our better understanding on the bacterial action for bioleaching of complex copper sulfide ores that remains debated so far as refractory ores.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":"68 1","pages":"1-1"},"PeriodicalIF":0.0,"publicationDate":"2018-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85137403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"ITA REGISTRATION FORM AND BACK COVER","authors":"I. Helianti","doi":"10.5454/mi.11.2.%p","DOIUrl":"https://doi.org/10.5454/mi.11.2.%p","url":null,"abstract":"","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":"181 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80284660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
W. Widanarni, Savini Retalia Sababalat, M. Yuhana, Diah A.S Utami
This study aimed to evaluate the effectiveness of the supplementation of Pseudoalteromonas piscisida 1UBthrough Artemia sp. to enhance the growth performance, immune response and the resistance of white shrimp ( Litopenaeus vannamei ) larvae to the infection of Vibrio harveyi . The natural feed given to the white shrimp larvae was Artemia sp. enriched with P. piscisida 1UB R at concentrations of 10 6 CFU mL -1 , 10 7 CFU mL -1 , 10 8 CFU mL -1 and a control ( Artemia sp. without any enrichment). The experimental shrimps (0.25±0.02 mg shrimp -1 ) were reared in the aquarium (25 × 20 × 30 cm) containing 4 L sea water with a stocking density of 30 shrimps L -1 . The experimental shrimps were fed the experimental feed from mysis 3 to PL12, and after that they were challenged with V. harveyi (10 7 CFU mL -1 )through an immersion method. The results of this study revealed that the administration of Artemia sp. enriched with P. piscisida 1UB could improve the survival, daily growth rate and absolute growth of length of white shrimp larvae. The activities of protease, lipase and amylase of white shrimp larvae treated with probiotic were higher (p<0.05) than those of the control. After the challenge test, white shrimp larvae treated with probiotic also had better survival and immune response (total hemocyte count, phagocytic activity, phenoloxidase activity and respiratory burst activity) than those of the the positive control. The best results were obtained in the probiotic application with a concentration of 10 8 CFU mL -1 .
本试验旨在评价通过青蒿素添加piscisida假异单胞菌1ubb对凡纳滨对虾(Litopenaeus vannamei)幼虾生长性能、免疫应答及对哈维弧菌(Vibrio harveyi)抗性的影响。白对虾幼虫的天然饲料为富含piscisida 1UB R的Artemia sp.，浓度分别为106 CFU mL - 1,107 CFU mL - 1,10 8 CFU mL -1和未富集的Artemia sp.。试验对虾(0.25±0.02 mg -虾-1)饲养在25 × 20 × 30 cm水族箱中，水族箱中含4 L海水，放养密度为30只虾-1。试验对虾从3号开始饲喂试验饲料至12号，然后通过浸渍法攻毒哈维氏弧菌(10 7 CFU mL -1)。本研究结果表明，添加富含piscisida 1UB的Artemia sp.可以提高白对虾的存活率、日生长率和绝对长高。经益生菌处理的白对虾幼虫蛋白酶、脂肪酶和淀粉酶活性均高于对照组(p<0.05)。攻毒试验后，经益生菌处理的白对虾幼虫的存活率和免疫应答(血细胞总数、吞噬活性、酚氧化酶活性和呼吸爆发活性)均高于阳性对照。以10 8 CFU mL -1浓度的益生菌施用效果最佳。
{"title":"The Administration of Pseudoalteromonas piscisida 1UB through Artemia sp. to Enhance Growth Performance, Immune Response and Resistance of White Shrimp (Litopenaeus vannamei) Larvae against Vibrio harveyi","authors":"W. Widanarni, Savini Retalia Sababalat, M. Yuhana, Diah A.S Utami","doi":"10.5454/MI.12.3.%P","DOIUrl":"https://doi.org/10.5454/MI.12.3.%P","url":null,"abstract":"This study aimed to evaluate the effectiveness of the supplementation of Pseudoalteromonas piscisida 1UBthrough Artemia sp. to enhance the growth performance, immune response and the resistance of white shrimp ( Litopenaeus vannamei ) larvae to the infection of Vibrio harveyi . The natural feed given to the white shrimp larvae was Artemia sp. enriched with P. piscisida 1UB R at concentrations of 10 6 CFU mL -1 , 10 7 CFU mL -1 , 10 8 CFU mL -1 and a control ( Artemia sp. without any enrichment). The experimental shrimps (0.25±0.02 mg shrimp -1 ) were reared in the aquarium (25 × 20 × 30 cm) containing 4 L sea water with a stocking density of 30 shrimps L -1 . The experimental shrimps were fed the experimental feed from mysis 3 to PL12, and after that they were challenged with V. harveyi (10 7 CFU mL -1 )through an immersion method. The results of this study revealed that the administration of Artemia sp. enriched with P. piscisida 1UB could improve the survival, daily growth rate and absolute growth of length of white shrimp larvae. The activities of protease, lipase and amylase of white shrimp larvae treated with probiotic were higher (p<0.05) than those of the control. After the challenge test, white shrimp larvae treated with probiotic also had better survival and immune response (total hemocyte count, phagocytic activity, phenoloxidase activity and respiratory burst activity) than those of the the positive control. The best results were obtained in the probiotic application with a concentration of 10 8 CFU mL -1 .","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":"41 1","pages":"92-98"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75237304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Pambudi, E. Mardliyati, S. Rahmani, D. Setyawati, T. Widayanti, A. Gill, A. Sulfianti, Whinie Lestari
The potency of aluminum hydroxide as an adjuvant in vaccine development is considered to depend on its particle size. In previous studies, we have successfully prepared two size particle, micro, and nano, aluminum hydroxide gel (alum) adjuvants. The potency of those particles as a candidate of adjuvant is needed to be characterized. In this study, we formulated our adjuvants with purified DENV3 pre Membrane Envelope (prM-E) recombinant protein and evaluated the induction of nitric oxide level in mouse macrophage RAW 264.7 cells. We prepared the alum adjuvant by precipitation-homogenization methods with an agitation rate at 11,000xg. Secreted prM-E recombinant protein was collected from Pichia pastoris X-33 fermentation which produced using bioreactor. Recombinant protein purification was carried out by anion exchange chromatography followed with size exclusion chromatography. The purified prM-E recombinant protein was observed as a single band around 70 -1k Da with a concentration of 105 mg mL . Complex nanoparticles alum with prM-E protein significantly (p<0.05) induced the nitric oxide level. Further analysis should be conducted in order to discover the detail molecular mechanism of nanoparticle alum adjuvant, recombinant protein, and cellular immune response.
{"title":"The Potency of Aluminum Hydroxide Nanoparticles for Dengue Subunit Vaccine Adjuvant","authors":"S. Pambudi, E. Mardliyati, S. Rahmani, D. Setyawati, T. Widayanti, A. Gill, A. Sulfianti, Whinie Lestari","doi":"10.5454/MI.12.3.5","DOIUrl":"https://doi.org/10.5454/MI.12.3.5","url":null,"abstract":"The potency of aluminum hydroxide as an adjuvant in vaccine development is considered to depend on its particle size. In previous studies, we have successfully prepared two size particle, micro, and nano, aluminum hydroxide gel (alum) adjuvants. The potency of those particles as a candidate of adjuvant is needed to be characterized. In this study, we formulated our adjuvants with purified DENV3 pre Membrane Envelope (prM-E) recombinant protein and evaluated the induction of nitric oxide level in mouse macrophage RAW 264.7 cells. We prepared the alum adjuvant by precipitation-homogenization methods with an agitation rate at 11,000xg. Secreted prM-E recombinant protein was collected from Pichia pastoris X-33 fermentation which produced using bioreactor. Recombinant protein purification was carried out by anion exchange chromatography followed with size exclusion chromatography. The purified prM-E recombinant protein was observed as a single band around 70 -1k Da with a concentration of 105 mg mL . Complex nanoparticles alum with prM-E protein significantly (p<0.05) induced the nitric oxide level. Further analysis should be conducted in order to discover the detail molecular mechanism of nanoparticle alum adjuvant, recombinant protein, and cellular immune response.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":"207 1","pages":"99-105"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83611833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The use of bacterial calcium carbonate (calcite) precipitation (biocementation) has recently become popular as a ground-improvement technique. Ureolytic bacteria having highly urease activities were known to have important roles in calcium carbonate precipitation process. One of our research objectives is to isolate and to select as many as possible such ureolytic bacteria from Indonesian soils to be further utilized for calcium carbonate (calcite) precipitation process in the soil for strengthening the soil structure. Isolation was performed anaerobically in selective media containing 40% urea. Four isolates with different morphologies were purified and coded as TK1, TK2, TK3, and TK4. Each of them was tested for its urease activity either as a pure culture or as a mixture of several cultures. The urease activity was measured based on the ammonia concentration produced in the growth media up to 7 x 24 hours. It was known that isolate TK4 had the highest urease activity on week 6, whilst a mixture of isolate cultures coded as TKC did not show a better urease activity than the isolate TK4. Hence, it could be concluded that the isolate TK4 was the best candidate to be used for further research on the calcium carbonate (calcite) precipitation process (biocementation) to strengthen the soil structure.
{"title":"Isolation and Urease Activity Test of Bacteria for Calcium Carbonate (Calcite) Precipitation (Biocementation) in Soil","authors":"H. Ambarsari, A. Ridlo","doi":"10.5454/MI.12.3.2","DOIUrl":"https://doi.org/10.5454/MI.12.3.2","url":null,"abstract":"The use of bacterial calcium carbonate (calcite) precipitation (biocementation) has recently become popular as a ground-improvement technique. Ureolytic bacteria having highly urease activities were known to have important roles in calcium carbonate precipitation process. One of our research objectives is to isolate and to select as many as possible such ureolytic bacteria from Indonesian soils to be further utilized for calcium carbonate (calcite) precipitation process in the soil for strengthening the soil structure. Isolation was performed anaerobically in selective media containing 40% urea. Four isolates with different morphologies were purified and coded as TK1, TK2, TK3, and TK4. Each of them was tested for its urease activity either as a pure culture or as a mixture of several cultures. The urease activity was measured based on the ammonia concentration produced in the growth media up to 7 x 24 hours. It was known that isolate TK4 had the highest urease activity on week 6, whilst a mixture of isolate cultures coded as TKC did not show a better urease activity than the isolate TK4. Hence, it could be concluded that the isolate TK4 was the best candidate to be used for further research on the calcium carbonate (calcite) precipitation process (biocementation) to strengthen the soil structure.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":"19 1","pages":"74-82"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76731985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Oil recovery test has been done by using crude biosurfactant from Brevundimonas diminuta and Bhurkholderia glumae indigenous halo tolerant bacteria with the vatiation of NaCl salt concentration 0; 1.5; 3; 4.5; 6; and 7.5%. Oil recovery test was obtained by determining % TPH (Total Petrolem Hidrocarbon). The sample concentration was 28.19% TPH, it was extracted by using biosurfactant of Brevundimonas diminuta and Bhurkholderia glumae bacteria, the optimal salitnity conditions were at 3, 4.5% salt concentrations with the value oil recovery as much as 50.41, 69.97 % respectively. Oil components which extraction by biosurfactant were analyzed by using GC-MS ( Gas Chromatography-Mass Spectrophotometry) . The result from analyzes GC-MS could be concluded that bacteria Brevundimonas diminuta could dissolve hydrocarbon compounds short chain carbon atom at fraction C 22. C 22 according to the retention time.
{"title":"Oil Recovery Test Using Bio surfactant of Halo tolerant Bacteria Brevundimonas diminuta and Bhurkholderia glumae at variation of NaCl Salt Concentrations","authors":"B. Yudono, M. Said, S. Estuningsih, A. Karima","doi":"10.5454/mi.11.3.2","DOIUrl":"https://doi.org/10.5454/mi.11.3.2","url":null,"abstract":"Oil recovery test has been done by using crude biosurfactant from Brevundimonas diminuta and Bhurkholderia glumae indigenous halo tolerant bacteria with the vatiation of NaCl salt concentration 0; 1.5; 3; 4.5; 6; and 7.5%. Oil recovery test was obtained by determining % TPH (Total Petrolem Hidrocarbon). The sample concentration was 28.19% TPH, it was extracted by using biosurfactant of Brevundimonas diminuta and Bhurkholderia glumae bacteria, the optimal salitnity conditions were at 3, 4.5% salt concentrations with the value oil recovery as much as 50.41, 69.97 % respectively. Oil components which extraction by biosurfactant were analyzed by using GC-MS ( Gas Chromatography-Mass Spectrophotometry) . The result from analyzes GC-MS could be concluded that bacteria Brevundimonas diminuta could dissolve hydrocarbon compounds short chain carbon atom at fraction C 22. C 22 according to the retention time.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":"56 1","pages":"2"},"PeriodicalIF":0.0,"publicationDate":"2017-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78941850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Staphylococcus aureus is one of major pathogens causing serious infection. Penicillin antibiotic is one of therapies against Staphylococcus infection. However, inadequate and irrational use of antibiotic causes resistance and emerges incidence of methicillin-resistant Staphylococcus aureus (MRSA). Herbal medicine from pineapple core extract is hopefully can reduce the incidence of antibiotics resistance. This research was conducted to investigate the antibacterial activity of pineapple core extract against MRSA. This research is true experimental with post-test controlled group design. Pineapple core was extracted by maceration method. Ethanol extract of pineapple core is dissolved with sterilized water and obtained concentration of 750, 500, 250, 187.5, 125, and 62.5 mg/ml. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by broth dilution test with five replications. Vancomycin was used as control group. MIC was observed visually by comparing turbidity of solutions after incubation at 37 o C for 24 hours. Then these solutions were cultured on nutrient agar plates at 37 o C for 24 hours. MBC was observed visually by inspecting the presence of bacterial colonies growth. The Minimum Inhibitory Concentration (MIC) could not be determined due to no turbidity changes. Vancomycin cannot be used for determining MIC. Cultures on nutrient agar plates had no colonies growth in concentrations of 750 and 500 mg/ml. Thus, the Minimum Bactericidal Concentration (MBC) was 500 mg/ml. Pineapple core extract contains bromelain, flavonoid, saponin, and tanin, which have antibacterial effect. In summary, pineapple core extract has antibacterial effect against methicillin-resistant Staphylococcus aureus (MRSA) with MBC of 500 mg/ml.
{"title":"Antibacterial Potentiality Testing of Pineapple Core Extract (Ananas comosus (L.) Merr) Against Methicillin-resistant Staphylococcus aureus (MRSA) with Vancomycin Control","authors":"B. P. Putra, D. Indiastuti, Deby Kusumaningrum","doi":"10.5454/MI.11.3.3","DOIUrl":"https://doi.org/10.5454/MI.11.3.3","url":null,"abstract":"Staphylococcus aureus is one of major pathogens causing serious infection. Penicillin antibiotic is one of therapies against Staphylococcus infection. However, inadequate and irrational use of antibiotic causes resistance and emerges incidence of methicillin-resistant Staphylococcus aureus (MRSA). Herbal medicine from pineapple core extract is hopefully can reduce the incidence of antibiotics resistance. This research was conducted to investigate the antibacterial activity of pineapple core extract against MRSA. This research is true experimental with post-test controlled group design. Pineapple core was extracted by maceration method. Ethanol extract of pineapple core is dissolved with sterilized water and obtained concentration of 750, 500, 250, 187.5, 125, and 62.5 mg/ml. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by broth dilution test with five replications. Vancomycin was used as control group. MIC was observed visually by comparing turbidity of solutions after incubation at 37 o C for 24 hours. Then these solutions were cultured on nutrient agar plates at 37 o C for 24 hours. MBC was observed visually by inspecting the presence of bacterial colonies growth. The Minimum Inhibitory Concentration (MIC) could not be determined due to no turbidity changes. Vancomycin cannot be used for determining MIC. Cultures on nutrient agar plates had no colonies growth in concentrations of 750 and 500 mg/ml. Thus, the Minimum Bactericidal Concentration (MBC) was 500 mg/ml. Pineapple core extract contains bromelain, flavonoid, saponin, and tanin, which have antibacterial effect. In summary, pineapple core extract has antibacterial effect against methicillin-resistant Staphylococcus aureus (MRSA) with MBC of 500 mg/ml.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":"24 1","pages":"3"},"PeriodicalIF":0.0,"publicationDate":"2017-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87969116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lactobacillus plantarum and Saccharomyces cerevisiae possess several of extracellular and intracellular of enzymes beneficial to cassava fermentation. Tapioka (cassava starch) has limited uses in food industries due to its low pasting properties, therefore, biomodification by the use of fermentation is needed. The research was aimed to monitor the growth of Saccharomyces cerevisiae and L. plantarum during tapioca fermentation, and to evaluate the chemical change, of the fermented tapioka. Mixed cultures was inoculated at the designed concentration into tapioca suspension and incubated at room temperature (30±2 o C) in facultative aerobic condition for 0, 24, 48, 60, 72, 96, 120, and 144 h. The growth change of S . cerevisiae and L. plantarum was monitored, and the change of pH, residual sugar, and starch granule was investigate. The result showed that S. cerevisiae had longer lag phase as well as stationary than L. plantarum was ; nevertheless , they both reached log phase at the same time. Co-inoculated mixed cultures did not affect the change on pH and reducing sugar but increased pronouncely protein content at stationary period. Besides, there was sign of erosion to the structure of cassava starch granules which was an indication of changes in the pasting property of the cassava starch.
{"title":"The Dynamic Growth and Chemical Change of Mixed Cultures Inoculation on Tapioka Fermentation","authors":"M. E. Kustyawati, A. Rangga, S. Setyani","doi":"10.5454/MI.11.3.5","DOIUrl":"https://doi.org/10.5454/MI.11.3.5","url":null,"abstract":"Lactobacillus plantarum and Saccharomyces cerevisiae possess several of extracellular and intracellular of enzymes beneficial to cassava fermentation. Tapioka (cassava starch) has limited uses in food industries due to its low pasting properties, therefore, biomodification by the use of fermentation is needed. The research was aimed to monitor the growth of Saccharomyces cerevisiae and L. plantarum during tapioca fermentation, and to evaluate the chemical change, of the fermented tapioka. Mixed cultures was inoculated at the designed concentration into tapioca suspension and incubated at room temperature (30±2 o C) in facultative aerobic condition for 0, 24, 48, 60, 72, 96, 120, and 144 h. The growth change of S . cerevisiae and L. plantarum was monitored, and the change of pH, residual sugar, and starch granule was investigate. The result showed that S. cerevisiae had longer lag phase as well as stationary than L. plantarum was ; nevertheless , they both reached log phase at the same time. Co-inoculated mixed cultures did not affect the change on pH and reducing sugar but increased pronouncely protein content at stationary period. Besides, there was sign of erosion to the structure of cassava starch granules which was an indication of changes in the pasting property of the cassava starch.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":"33 1","pages":"2"},"PeriodicalIF":0.0,"publicationDate":"2017-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89459711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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