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List of reviewers 审稿人列表。
IF 2.6 4区 医学 Q3 Immunology and Microbiology Pub Date : 2023-12-07 DOI: 10.1111/1348-0421.13105
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引用次数: 0
Association of ATG16L1 and ATG5 gene polymorphisms with susceptibility to hepatitis B virus infection and progression to HCC in central China 中国中部地区ATG16L1和ATG5基因多态性与乙型肝炎病毒感染易感性和HCC进展的关系
IF 2.6 4区 医学 Q3 Immunology and Microbiology Pub Date : 2023-11-22 DOI: 10.1111/1348-0421.13104
Qiaoyu Wu, Yaoling Ouyang

Hepatitis B virus (HBV) infection is a severe public health problem worldwide. The relationship between polymorphisms of autophagy-related 16-like 1 gene (ATG16L1) and autophagy-related gene 5 (ATG5) with susceptibility to the stage of HBV infection has been reported in different populations. Nevertheless, this association is not seen in the population of central China. This study recruited 452 participants, including 246 HBV-infected patients (139 chronically infected HBV without hepatocellular carcinoma [HCC] and 107 HBV-related HCC patients) and 206 healthy controls. Genotyping of ATG16L1 rs2241880 and ATG5 rs688810 were performed using Sanger sequencing and polymerase chain reaction-restriction fragment length polymorphism, respectively. Our results indicated that the G allele of ATG16L1 rs2241880 was more frequent in healthy controls than in patients with chronicHBV infection. After adjusting for age and sex, an association between the ATG16L1 rs2241880 polymorphism and HBV infection was significant under the dominant and allele models (p = 0.009 and 0.003, respectively). However, no association between the ATG5 polymorphisms and HBV infection was observed. We also did not find a significant association between ATG16L1 and ATG5 polymorphisms and the progression of HBV-related HCC. Therefore, the genetic polymorphism of ATG16L1 rs2241880 may be associated with susceptibility to HBV infection in the population of central China.

乙型肝炎病毒(HBV)感染是世界范围内严重的公共卫生问题。自噬相关16-样1基因(ATG16L1)和自噬相关基因5 (ATG5)多态性与HBV感染阶段易感性的关系在不同人群中已有报道。然而,这种关联在中国中部人群中未见。该研究招募了452名参与者,包括246名HBV感染患者(139名慢性HBV感染无肝细胞癌[HCC]和107名HBV相关HCC患者)和206名健康对照。ATG16L1 rs2241880和ATG5 rs688810分别采用Sanger测序和聚合酶链反应-限制性片段长度多态性进行基因分型。我们的研究结果表明,ATG16L1 rs2241880的G等位基因在健康对照中比在慢性乙型肝炎感染患者中更常见。在调整年龄和性别后,在显性和等位基因模型下,ATG16L1 rs2241880多态性与HBV感染之间存在显著关联(p分别= 0.009和0.003)。然而,ATG5多态性与HBV感染之间没有关联。我们也没有发现ATG16L1和ATG5多态性与hbv相关HCC进展之间的显著关联。因此,ATG16L1 rs2241880基因多态性可能与中国中部人群对HBV感染的易感性有关。
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引用次数: 0
Biological characteristics and pathogenicity of a Staphylococcus aureus strain with an incomplete hemolytic phenotype isolated from bovine milk 牛乳中分离的一株不完全溶血性金黄色葡萄球菌的生物学特性和致病性。
IF 2.6 4区 医学 Q3 Immunology and Microbiology Pub Date : 2023-11-20 DOI: 10.1111/1348-0421.13102
Xiuhua Xu, Tingting Zhou, Xueyao Fang, Longhua Hu, Jin Zhu, Feng Zheng

Staphylococcus aureus is a common pathogen capable of infecting both humans and animals and causing various severe diseases. Here, we aimed to determine the biological features and pathogenicity of S. aureus strain Sa9, of the incomplete hemolysis phenotype, isolated from bovine milk. Sa9 was classified as ST97 by multilocus sequence typing, and it showed increased β-hemolysin expression and lower Hla and Hld expression levels compared with that in the S. aureus USA300 strain LAC. RT-PCR and ELISA results showed that the expression levels of inflammatory cytokines were higher in Sa9-induced mouse primary peritoneal macrophages compared with those induced by the LAC strain. However, the Sa9 strain also mediated anti-inflammatory effects by upregulating IL-10 and IFN-β in macrophages, which were not apparently induced by S. aureus culture supernatants. Phagocytosis and whole-blood survival assays were also performed to assess the in vitro survival of bacteria, and the virulence was evaluated in mice. Although the Sa9 strain showed lower ability of intracellular survival in macrophages than LAC, similar multiplication in human whole blood and pathogenicity toward mice were observed. Taken together, we report that the distinctive immune response induced by the S. aureus strain with an incomplete hemolysis phenotype occurs in cattle, and its potential pathogenicity and risk of transmission to humans require attention.

金黄色葡萄球菌是一种常见的病原体,能够感染人和动物,引起各种严重疾病。在这里,我们旨在确定从牛乳中分离的不完全溶血表型金黄色葡萄球菌Sa9菌株的生物学特性和致病性。通过多位点序列分型将Sa9归为ST97,与金黄色葡萄球菌USA300菌株LAC相比,Sa9 β-溶血素表达升高,Hla和Hld表达水平降低。RT-PCR和ELISA结果显示,sa9诱导的小鼠原代腹腔巨噬细胞中炎症因子的表达水平高于LAC诱导的小鼠。然而,Sa9菌株也通过上调巨噬细胞中的IL-10和IFN-β介导抗炎作用,而金黄色葡萄球菌培养上清液对这一作用的诱导作用并不明显。通过吞噬和全血存活试验评估细菌的体外存活,并对小鼠进行毒力评估。虽然Sa9菌株在巨噬细胞中的细胞内存活能力低于LAC,但在人全血中的增殖和对小鼠的致病性相似。综上所述,我们报告了由具有不完全溶血表型的金黄色葡萄球菌菌株诱导的独特免疫反应发生在牛身上,其潜在的致病性和传播给人类的风险需要引起注意。
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引用次数: 0
Pneumococcus downregulates the molecular weight of the extracellular domain of the epidermal growth factor receptor of alveolar epithelial cells 肺炎球菌下调肺泡上皮细胞表皮生长因子受体胞外结构域的分子量。
IF 2.6 4区 医学 Q3 Immunology and Microbiology Pub Date : 2023-11-17 DOI: 10.1111/1348-0421.13103
Toshihito Isono, Satoru Hirayama, Hisanori Domon, Yutaka Terao

Pneumococcus is themajor cause of bacterial and invasive pneumococcal infections. Disrupting the alveolarepithelial barrier is an important step in the pathogenesis of invasivepneumococcal infections. The epidermal growth factor receptor (EGFR) maintainsthe integrity of the alveolar epithelial barrier. In this study, we showed that secretory pneumococcal molecules decrease the molecular weight of EGFR without peptide degradation and inhibit alveolar epithelial cell proliferation via EGFR.

肺炎球菌是细菌性和侵袭性肺炎球菌感染的主要原因。破坏肺泡上皮屏障是侵袭性肺炎球菌感染发病机制的重要步骤。表皮生长因子受体(EGFR)维持肺泡上皮屏障的完整性。在这项研究中,我们发现,分泌性肺炎球菌分子降低EGFR的分子量而不降解肽,并通过EGFR抑制肺泡上皮细胞的增殖。
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引用次数: 0
Modulatory effects of supplementation of Lentinula edodes mycelia extract and l-arginine on the therapeutic efficacy of immunogenic chemotherapy in colon cancer-bearing mice 补充香菇菌丝提取物和l-精氨酸对结肠癌小鼠免疫原性化疗疗效的调节作用。
IF 2.6 4区 医学 Q3 Immunology and Microbiology Pub Date : 2023-11-14 DOI: 10.1111/1348-0421.13101
Takahito Taniura, Kazunari Ishitobi, Masaaki Hidaka, Mamoru Harada

Some chemotherapeutic drugs can induce cancer cell death and enhance antitumor T-cell immunity in cancer-bearing hosts. Immunomodulatory reagents could augment such chemotherapy-induced effects. We previously reported that oral digestion of Lentinula edodes mycelia (L.E.M.) extract or l-arginine supplementation can augment antitumor T-cell responses in cancer-bearing mice. In this study, the effects of L.E.M. extract with or without l-arginine on the therapeutic efficacy of immunogenic chemotherapy by 5-fluorouracil (5-FU)/oxaliplatin (L-OHP) and/or cyclophosphamide (CP) are examined using two mouse colon cancer models. In MC38 and CT26 cancer models, therapy with 5-FU/L-OHP/CP significantly suppressed tumor growth, and supplementation with L.E.M. extract halved the tumor volumes. However, the modulatory effect of L.E.M. extract was not significant. In the CT26 cancer model, supplementation with L.E.M. extract and l-arginine had no clear effect on tumor growth. In contrast, their addition to chemotherapy halved the tumor volumes, although the effect was not significant. There was no difference in the cytotoxicity of tumor-specific cytotoxic T cells generated from CT26-cured mice treated by chemotherapy alone versus chemotherapy combined with L.E.M. extract/l-arginine. These results indicate that the antitumor effects of immunogenic chemotherapy were too strong to ascertain the effects of supplementation of L.E.M. extract and l-arginine, but these reagents nonetheless have immunomodulatory effects on the therapeutic efficacy of immunogenic chemotherapy in colon cancer-bearing mice.

一些化疗药物可以诱导癌细胞死亡,增强肿瘤宿主的抗肿瘤t细胞免疫。免疫调节试剂可以增强这种化疗诱导的效应。我们之前报道过,口服消化香菇菌丝体(L.E.M.)提取物或补充l-精氨酸可以增强患癌小鼠的抗肿瘤t细胞反应。本研究采用两种小鼠结肠癌模型,研究了加或不加l-精氨酸对5-氟尿嘧啶(5-FU)/奥沙利铂(L-OHP)和/或环磷酰胺(CP)免疫原性化疗疗效的影响。在MC38和CT26癌症模型中,5-FU/L-OHP/CP治疗可显著抑制肿瘤生长,补充leem提取物可使肿瘤体积减半。而枸杞提取物的调节作用不显著。在CT26肿瘤模型中,补充leem提取物和l-精氨酸对肿瘤生长无明显影响。相比之下,他们在化疗的基础上使肿瘤体积减半,尽管效果并不显著。单独化疗与化疗联合e.m.提取物/ l-精氨酸治疗ct26治愈小鼠产生的肿瘤特异性细胞毒性T细胞的细胞毒性没有差异。这些结果表明,免疫原性化疗的抗肿瘤作用太强,无法确定补充leemm提取物和l-精氨酸的效果,但这些试剂对免疫原性化疗对结肠癌小鼠的治疗效果具有免疫调节作用。
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引用次数: 0
Issue Information – Cover 发行资料-封面
IF 2.6 4区 医学 Q3 Immunology and Microbiology Pub Date : 2023-11-08 DOI: 10.1111/1348-0421.13008

Cover photograph: Confocal fluorescence pictures of cultured macrophages stained using different clones of CD169 (blue: DAPI; green: HSn7D2; red: SP216). Microbiol Immunol: 67:490–500. Article link here

封面图片:用不同克隆CD169染色的培养巨噬细胞的共聚焦荧光图片(蓝色:DAPI;绿色:HSn7D2;红色:SP216)。中华微生物学杂志(英文版):67:490-500。文章链接
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引用次数: 0
Genomic characterization of New Delhi metallo-beta-lactamase–producing species of Morganellaceae, Yersiniaceae, and Enterobacteriaceae (other than Klebsiella) from Brazil over 2013–2022 2013-2022年来自巴西的Morganellaceae、Yersiniaceae和肠杆菌科(克雷伯菌除外)新德里金属β-内酰胺酶产生种的基因组特征。
IF 2.6 4区 医学 Q3 Immunology and Microbiology Pub Date : 2023-10-19 DOI: 10.1111/1348-0421.13100
Carlos Henrique Camargo, Amanda Yaeko Yamada, Andreia Rodrigues de Souza, Claudio Tavares Sacchi, Alex Domingos Reis, Marlon Benedito Nascimento Santos, Denise Brandão de Assis, Eneas de Carvalho, Elizabeth Harummyy Takagi, Marcos Paulo Vieira Cunha, Monique Ribeiro Tiba-Casas

Over the last decade, New Delhi metallo-beta-lactamase (NDM) carbapenemase has silently spread in Brazil. In this study, we analyzed a large collection of Enterobacterales other than Klebsiella spp. received in our reference laboratory between 2013 and 2022. A total of 32 clinical isolates displaying different pulsed-field gel electrophoresis profiles, and represented by 11 species in the families Enterobacteriaceae (Citrobacter freundii, Citrobacter portucalensis, Enterobacter hormaechei, and Escherichia coli), Morganellaceae (Morganella morganii, Proteus mirabilis, Proteus vulgaris, Providencia rettgeri, Providencia stuartii, and Raoultella ornithinolytica), and Yersiniaceae (Serratia marcescens) had their whole genomes sequenced and further analyzed. Antimicrobial susceptibility was determined by disk diffusion, except for polymyxin B, assessed by broth microdilution. The blaNDM-1 allele was predominant (n = 29), but blaNDM-5 was identified in an E. coli specimen with a novel ST, and the blaNDM-7 allele was found in E. hormaechei ST45 and E. coli ST1049. Polymyxin was active against all but one Enterobacteriaceae isolate: an mcr-1–producing E. coli presenting minimal inhibitory concentration (4 mg/L). Isolates producing extended-spectrum β-lactamases were common: cefotaximase from Munich (CTX-M)-15 (n = 10), CTX-M-2 (n = 4), and CTX-M-8 (n = 3) were detected, and the mcr-1–producing E. coli was found to co-produce both CTX-M-8 and CTX-M-55 β-lactamases. The mcr-9 gene was found in 5/8 E. hormaechei isolates, distributed in four different sequence types, all of them presenting susceptibility to polymyxin. This study showed that NDM-producing Enterobacterales other than Klebsiella are already spread in Brazil, in diversified species, and cocarrying important resistance genes. Prompt detection and effective implementation of measures to prevent further spread are mandatory for mitigating the dissemination of NDM carbapenemase in hospital settings and preserving the already limited antimicrobial therapy options.

在过去的十年里,新德里金属β-内酰胺酶(NDM)碳青霉烯酶在巴西悄然传播。在这项研究中,我们分析了2013年至2022年间在我们的参考实验室收到的除克雷伯菌属以外的大量肠杆菌。共有32个临床分离株显示出不同的脉冲场凝胶电泳图谱,以肠杆菌科的11个种为代表(弗氏柠檬杆菌、葡萄牙柠檬杆菌、霍马切肠杆菌和大肠杆菌),Morganellaceae(Morganella morganii、奇异变形菌、普通变形菌、Providencia rettgeri、Providenciastuartii和Raoultella ornithnolytica)和耶尔森菌科(粘质沙雷氏菌)的全基因组测序并进一步分析。除多粘菌素B外,通过纸片扩散法测定抗菌药物敏感性,通过肉汤微量稀释法评估。blaNDM-1等位基因占优势(n = 29),但在具有新ST的大肠杆菌样本中鉴定出blaNDM-5,并且在E.hormaechei ST45和E.coli ST1049中发现了blaNDM-7等位基因。多粘菌素对除一种外的所有肠杆菌科分离株都有活性:一种mcr-1产生的大肠杆菌,具有最低的抑制浓度(4 mg/L)。产生超广谱β-内酰胺酶的分离株很常见:来自慕尼黑的头孢噻肟酶(CTX-M)-15(n = 10) ,CTX-M-2(n = 4) 和CTX-M-8(n = 3) 并且发现产生mcr-1的大肠杆菌同时产生CTX-M-8和CTX-M-55β-内酰胺酶。mcr-9基因在5/8个荷玛氏大肠杆菌分离株中发现,分布在四种不同的序列类型中,均表现出对多粘菌素的易感性。这项研究表明,除克雷伯菌外,产生NDM的肠杆菌已经在巴西以多样化的物种传播,并共同携带重要的抗性基因。为了减少NDM碳青霉烯酶在医院的传播,并保留已经有限的抗菌治疗选择,必须及时检测并有效实施预防进一步传播的措施。
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引用次数: 0
Natto extract inhibits infection caused by the Aujeszky's disease virus in mice 纳豆提取物可抑制小鼠感染奥耶斯基病病毒。
IF 2.6 4区 医学 Q3 Immunology and Microbiology Pub Date : 2023-10-10 DOI: 10.1111/1348-0421.13099
Junya Kobayashi, Rongduo Wen, Takanobu Nishikawa, Yuka Nunomura, Takehito Suzuki, Yudai Sejima, Toshiya Gokan, Makio Furukawa, Tomoko Yokota, Nanako Osawa, Yoko Sato, Yutaka Nibu, Tetsuya Mizutani, Mami Oba

Aujeszky's disease virus (ADV), also known as Suid alphaherpesvirus 1, which mainly infects swine, causes life-threatening neurological disorders. This disease is a serious global risk factor for economic losses in the swine industry. The development of new anti-ADV drugs is highly anticipated and required. Natto, a traditional Japanese fermented food made from soybeans, is a well-known health food. In our previous study, we confirmed that natto has the potential to inhibit viral infections by severe acute respiratory syndrome coronavirus 2 and bovine alphaherpesvirus 1 through their putative serine protease(s). In this study, we found that an agent(s) in natto functionally impaired ADV infection in cell culture assays. In addition, ADV treated with natto extract lost viral infectivity in the mice. We conducted an HPLC gel-filtration analysis of natto extract and molecular weight markers and confirmed that Fraction No. 10 had ADV-inactivating ability. Furthermore, the antiviral activity of Fraction No. 10 was inhibited by the serine protease inhibitor 4-(2-Aminoethyl) benzene sulfonyl fluoride hydrochloride (AEBSF). These results also suggest that Fraction No. 10, adjacent to the 12.5 kDa peak of the marker in natto extract, may inactivate ADV by proteolysis. Our findings provide new avenues of research for the prevention of Aujeszky's disease.

奥耶斯基病病毒(ADV),也称为Suidα疱疹病毒1型,主要感染猪,会导致危及生命的神经系统疾病。这种疾病是造成养猪业经济损失的严重全球风险因素。抗ADV新药的开发备受期待和要求。纳豆是一种由大豆制成的日本传统发酵食品,是一种著名的健康食品。在我们之前的研究中,我们证实纳豆有可能通过其假定的丝氨酸蛋白酶抑制严重急性呼吸综合征冠状病毒2型和牛α疱疹病毒1型的病毒感染。在这项研究中,我们发现纳豆中的一种制剂在细胞培养试验中功能性地损害了ADV感染。此外,用纳豆提取物处理的ADV在小鼠中失去了病毒感染性。我们对纳豆提取物和分子量标记物进行了HPLC凝胶过滤分析,并确认馏分10具有ADV灭活能力。此外,组分10的抗病毒活性被丝氨酸蛋白酶抑制剂4-(2-氨基乙基)苯磺酰氟盐酸盐(AEBSF)抑制。这些结果还表明,与12.5 纳豆提取物中标记物的kDa峰可能通过蛋白水解失活ADV。我们的发现为预防Aujeszky病提供了新的研究途径。
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引用次数: 0
Bioinformatic investigation of Nipah virus surface protein mutations: Molecular docking with Ephrin B2 receptor, molecular dynamics simulation, and structural impact analysis 尼帕病毒表面蛋白突变的生物信息学研究:与Ephrin B2受体的分子对接、分子动力学模拟和结构影响分析。
IF 2.6 4区 医学 Q3 Immunology and Microbiology Pub Date : 2023-10-09 DOI: 10.1111/1348-0421.13098
Emre Aktaş, İrem Saygılı, Elif Kahveci, Zeynep Tekbıyık, Nehir Özdemir Özgentürk
The SARS‐CoV‐2 outbreak resulted in significant challenges and loss of life. The Nipah virus, known for its high infectivity and severity, was designated an emergency concern by the World Health Organization. To understand its mutations, the Nipah virus proteins were analyzed extensively, with a focus on the essential G and F proteins responsible for viral entry into host cells. Our bioinformatics analysis unveiled multiple mutations, including simultaneous mutations within a single sequence. Notably, the G273S mutation in the F protein was identified as a potential cause of structural damage, which carries significant implications for vaccine development. Comparing the docking scores of G and F proteins with the Ephrin B2 receptor, it was found that the Y228H mutation in the G protein and the D252G mutation in the F protein likely affect virus entry into host cells. Moreover, our investigation into stability and deformability highlighted the impact of the Y228H mutation in the G protein complex. Molecular dynamics simulations revealed increased flexibility and conformational changes in the G protein complex with the Y228H mutation compared with the known complex. Furthermore, evaluating the root mean square deviation variation demonstrated greater dynamic behavior in the G protein complex and the Ephrin B2 receptor complex. This comprehensive study provides valuable insights into Nipah virus mutations, their significance for vaccine development, and the importance of understanding protein complex behavior in drug discovery. The identified mutations, especially G273S and Y228H, hold crucial implications for future research and potential interventions against the Nipah virus.
严重急性呼吸系统综合征冠状病毒2型的爆发带来了重大挑战和生命损失。尼帕病毒以其高传染性和严重性而闻名,被世界卫生组织指定为紧急关注对象。为了了解其突变,对尼帕病毒蛋白进行了广泛分析,重点是负责病毒进入宿主细胞的必需G和F蛋白。我们的生物信息学分析揭示了多个突变,包括单个序列中的同时突变。值得注意的是,F蛋白中的G273S突变被确定为结构损伤的潜在原因,这对疫苗开发具有重要意义。比较G和F蛋白与Ephrin B2受体的对接得分,发现G蛋白中的Y228H突变和F蛋白中的D252G突变可能影响病毒进入宿主细胞。此外,我们对稳定性和可变形性的研究强调了G蛋白复合体中Y228H突变的影响。分子动力学模拟显示,与已知的复合物相比,具有Y228H突变的G蛋白复合物的灵活性和构象变化增加。此外,评估均方根偏差变化表明G蛋白复合物和Ephrin B2受体复合物具有更大的动态行为。这项全面的研究为尼帕病毒突变、其对疫苗开发的意义以及了解蛋白质复合物行为在药物发现中的重要性提供了有价值的见解。已确定的突变,特别是G273S和Y228H,对未来的研究和针对尼帕病毒的潜在干预措施具有重要意义。
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引用次数: 0
Issue Information – Cover 问题信息-封面
IF 2.6 4区 医学 Q3 Immunology and Microbiology Pub Date : 2023-10-04 DOI: 10.1111/1348-0421.13005

Cover photograph: Inflammasome activation by Listeria monocytogenes infection. Microbiol Immunol: 67:429–437. Article link here

封面照片:单核细胞增多性李斯特菌感染引起的炎症小体活化。微生物免疫学:67:429-437。此处的文章链接
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引用次数: 0
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Microbiology and Immunology
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