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Drug resistance in chronic myeloid leukemia: the involvement of Galectin-1 and Galectin-3. 慢性髓系白血病耐药:半乳糖凝集素-1和半乳糖凝集素-3的参与。
IF 2.8 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-20 DOI: 10.1007/s11033-026-11473-y
Cansu Yıldırım

Tyrosine kinase inhibitors (TKIs) have proven effective in treating chronic myeloid leukemia (CML), but some patients do not benefit from these drugs because TKI-resistant CML cells persist. Galectin-1 (Gal-1) and Galectin-3 (Gal-3) have emerged as critical modulators of CML cell drug resistance. High intracellular Gal-1 levels promote multidrug resistance in vitro by inducing MDR1 expression through p38 MAPK and NF-κB activation, enhancing drug efflux and diminishing anticancer drug efficacy. The effects of Gal-1 have so far been investigated only in in vitro systems; data from in vivo mouse and human studies are not available. Activation of GSK-3β induces Gal-3, which stabilizes anti-apoptotic proteins belonging to the Bcl-2 family and confers resistance to apoptosis-inducing agents in vitro. However, this pathway has not yet been evaluated in CML mouse models or in patients, particularly in the context of TKI resistance mechanisms. Gal-3 is induced by the bone marrow microenvironment (BMME) and promotes AKT and ERK activation to support CML cell proliferation, multidrug resistance (MDR), chemotaxis, and BM lodgment. However, it has not yet been elucidated how Gal-3 is induced by BM stromal cells in CML cells. Furthermore, Gal-3 suppresses the formation of the SERPINA1-albumin complex in vitro, abolishing its growth-inhibitory effects and enhancing paracrine proliferation of CML cells. Although preclinical evidence shows that elevated intracellular Gal-3 protein levels promote drug resistance in CML, these findings have not yet been confirmed at the clinical level. This review underscores that both galectins are critical regulators of CML pathophysiology and highlight their potential as therapeutic targets to overcome TKI resistance.

酪氨酸激酶抑制剂(TKIs)已被证明对治疗慢性髓性白血病(CML)有效,但由于tki耐药的CML细胞持续存在,一些患者不能从这些药物中获益。半乳糖凝集素-1 (Gal-1)和半乳糖凝集素-3 (Gal-3)已成为CML细胞耐药的关键调节剂。高水平的细胞内Gal-1通过p38 MAPK和NF-κB活化诱导MDR1表达,增强药物外排,降低抗癌药物疗效,从而促进体外多药耐药。迄今为止,Gal-1的作用仅在体外系统中进行了研究;没有来自小鼠和人类体内研究的数据。GSK-3β的激活诱导Gal-3,其稳定属于Bcl-2家族的抗凋亡蛋白,并在体外对凋亡诱导药物产生抗性。然而,这一途径尚未在CML小鼠模型或患者中进行评估,特别是在TKI耐药机制的背景下。Gal-3由骨髓微环境(BMME)诱导,促进AKT和ERK活化,支持CML细胞增殖、多药耐药(MDR)、趋化性和骨髓沉积。然而,目前尚不清楚Gal-3是如何在CML细胞中被BM基质细胞诱导的。此外,Gal-3在体外抑制serpina1 -白蛋白复合物的形成,消除其生长抑制作用,增强CML细胞的旁分泌增殖。尽管临床前证据表明细胞内Gal-3蛋白水平升高可促进CML的耐药,但这些发现尚未在临床水平上得到证实。这篇综述强调了这两种凝集素都是CML病理生理的关键调节因子,并强调了它们作为克服TKI耐药性的治疗靶点的潜力。
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引用次数: 0
Nipah and chandipura viruses: Emerging neurotropic zoonotic viruses in south asia - a comparative review. 尼帕病毒和钱迪普拉病毒:南亚新出现的嗜神经人畜共患病毒——比较综述。
IF 2.8 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-20 DOI: 10.1007/s11033-026-11469-8
Mustak Ahamed, Zuber Khan, Mumtaz, Sidharth Mehan, Nainsi Kumari, Aditya Raj, Manjeet Kumar
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引用次数: 0
Interplay between NOD1 polymorphisms and gene expression in the pathogenesis of gallstone disease. NOD1多态性与基因表达在胆结石发病机制中的相互作用
IF 2.8 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-20 DOI: 10.1007/s11033-026-11465-y
Sanjana Murali, Shanthi Vijayaraghavan, Rajesh Kumar Shanmugam, Nandini Krishnamurthy, R B Devi Krishna, Pradeep Narahari Ram, Jashwanth Balu, Andrea Mary Francis
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引用次数: 0
Exosomal crosstalk between breast cancer cells and tumor-associated macrophages: mechanisms and therapeutic implications. 乳腺癌细胞和肿瘤相关巨噬细胞之间的外泌体串扰:机制和治疗意义。
IF 2.8 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-20 DOI: 10.1007/s11033-026-11483-w
Rong-Quan Gong, An Xu, Xiao Huang, Deyuan Fu
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引用次数: 0
EGFR mutation spectrum and clinical correlations in Tunisian Non-Small cell lung cancer patients. 突尼斯非小细胞肺癌患者EGFR突变谱及临床相关性
IF 2.8 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-20 DOI: 10.1007/s11033-026-11472-z
Rania Abdelmaksoud-Dammak, Nihel Ammous-Boukhris, Slim Charfi, Nesrine Kallel, Rim Khemakhem, Ameni Feki, Tahya Sellami Boudawara, Ilhem Yangui, Afef Khanfir, Raja Mokdad Gargouri
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引用次数: 0
Molecular identification of pelagic fish eggs with oil globule. 含油球的远洋鱼卵的分子鉴定。
IF 2.8 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-20 DOI: 10.1007/s11033-026-11481-y
Nicolás Valladares, Paola González-Kother, Guillermo Herrera, Oscar Venegas, Luisana Lugo-Pérez, Maribet Gamboa

Background: Marine fish eggs exhibit transversal morphological characteristics that complicate their identification. It is estimated that 75% of pelagic eggs possess oil globule(s). Identifying these eggs provides valuable information about spawning biomass and potential spawning areas for commercially important and threatened species. Molecular markers, such as the FISH (COI gene), enable a precise identification and exploration of the genetic variability within fish populations. San Vicente Bay, located in south-central Chile is partially protected from the effects of the Humboldt current, making it a notable spawning and nursery ground for various fish species. This study aims to identify and characterize, oil-globule-containing eggs present in the bay during October, a period that aligns with the spawning season of many species.

Methods and results: This study seek to standardize the DNA extraction method and PCR process, addressing the challenges posed by the low quantities of tissue in these eggs. We employed 2 methods, by a DNA extraction kit and Chelex resin. Out of 94 eggs, 24 samples yielded positive PCR results, corresponding to a 25% of success. All these eggs were successfully identified using genetic databases, revealing a total of 9 genera and 8 species. The observed genetic distances at the species level were low, supporting the hypothesis that the eggs originated from the same female or gene mutation rate.

Conclusion: The methods employed successfully identified fish eggs and documented species whose early life stages have not been previously reported in the bay.

背景:海鱼卵表现出横向形态特征,使其识别复杂化。据估计,75%的远洋鱼卵含有油球。识别这些卵提供了关于产卵生物量和潜在产卵区域的有价值的信息,这些产卵区域对商业上重要的和受威胁的物种来说是潜在的。分子标记,如FISH (COI基因),可以精确识别和探索鱼类种群内的遗传变异性。圣维森特湾位于智利中南部,受到洪堡洋流的部分保护,使其成为各种鱼类的产卵和苗场。这项研究的目的是识别和表征十月海湾中含有油球的卵,这段时间与许多物种的产卵季节一致。方法和结果:本研究旨在标准化DNA提取方法和PCR过程,解决这些卵子中组织数量少所带来的挑战。我们采用了两种方法,DNA提取试剂盒和Chelex树脂。在94个卵子中,24个样本的PCR结果呈阳性,对应于25%的成功率。所有卵均通过遗传数据库成功鉴定,共鉴定出9属8种。在物种水平上观察到的遗传距离较低,支持了卵来自同一雌性或基因突变率的假设。结论:所采用的方法成功地鉴定了该海湾早期生活阶段未被报道的鱼卵和记录的物种。
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引用次数: 0
Process and analytical strategies for the safe production of mRNA vaccines and therapeutics. mRNA疫苗和疗法安全生产的过程和分析策略。
IF 2.8 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-20 DOI: 10.1007/s11033-026-11455-0
Cinderella J A Nowak, Sha Liu, Robert J Falconer, Lukas Gerstweiler
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引用次数: 0
The role of immune cell crosstalk and signaling pathways in the pathogenesis of pulmonary fibrosis. 免疫细胞串扰和信号通路在肺纤维化发病机制中的作用。
IF 2.8 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-19 DOI: 10.1007/s11033-026-11437-2
Xia Liu, Haixu Chen, Changying Guo, Yu Lin, Fei Wang, Zhi Lan
{"title":"The role of immune cell crosstalk and signaling pathways in the pathogenesis of pulmonary fibrosis.","authors":"Xia Liu, Haixu Chen, Changying Guo, Yu Lin, Fei Wang, Zhi Lan","doi":"10.1007/s11033-026-11437-2","DOIUrl":"https://doi.org/10.1007/s11033-026-11437-2","url":null,"abstract":"","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"53 1","pages":"299"},"PeriodicalIF":2.8,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145998610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isolation, whole genome sequencing, and comparative genomics of Corynebacterium pseudotuberculosis to identify biovar ovis specific biomarkers. 假结核棒状杆菌的分离、全基因组测序和比较基因组学鉴定卵巢生物变异特异性生物标志物。
IF 2.8 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-19 DOI: 10.1007/s11033-026-11471-0
Kumaragurubaran Karthik, Subbaiyan Anbazhagan, Murugesan Ananda Chitra, Ramaswamy Sridhar
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引用次数: 0
Novel mega-deletion in BFSP1 causing autosomal recessive juvenile cataract in a Pakistani consanguineous family. 在巴基斯坦的一个近亲家庭中,新的巨大缺失的BFSP1导致常染色体隐性青少年白内障。
IF 2.8 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-19 DOI: 10.1007/s11033-025-11427-w
Neelam Saba, Ambreen Kanwal, Saba Irshad

Background: Juvenile cataract is characterized by the blurredness in eye lens which develops typically before the age of 18 years. Objective of the study was to delineate the underlying causative genetic defect for autosomal recessive juvenile cataract (ARJC) in an affected consanguineous multiplex, multigenerational Pakistani consanguineous family.

Methods and results: Whole-exome sequencing (WES) in an affected family member revealed a homozygous, intragenic 15.40 kb mega-deletion NG_012423.2; g.17485448_17500744del (chr20: 17,485,448-17,500,744-GRCh37/hg19) including three consecutive exons 3, 4, and 5, encompassing two domains of beaded filament structural protein 1 (BFSP1). Sanger sequencing and PCR were performed for all participants of the family which revealed the absence of the exons (3, 4, 5) in affected individuals and their presence in unaffected parents and siblings confirming the segregation of the mutation as autosomal recessive in the family under study. The large deletion potentially forced the junction of exon 2 with exon 6 at mRNA level and resulting in the shift of open reading frame. The mutation was not observed in 200 unrelated in-house controls.

Conclusion: Up to our knowledge, this is the first study reporting the largest mega-deletion encompassing 15.40 kb removal (consisting of 99 amino acids) and reveals the significant role of BFSP1 for physiologic lens function and optical properties. Our findings extend the genotypic spectrum of BFSP1-related cataract.

背景:青少年白内障的特点是眼晶状体模糊,通常发生在18岁之前。本研究的目的是在一个受影响的多代巴基斯坦近亲家庭中描述常染色体隐性遗传性青少年白内障(ARJC)的潜在致病遗传缺陷。方法和结果:在一个受影响的家族成员中进行全外显子组测序(WES),发现一个纯合子,基因内15.40 kb的大缺失NG_012423.2;g.17485448_17500744del (chr20: 17,485,448-17,500,744-GRCh37/hg19),包含三个连续的外显子3,4,5,包含珠状丝结构蛋白1 (BFSP1)的两个结构域。对该家族的所有参与者进行Sanger测序和PCR,结果显示受影响个体中没有外显子(3,4,5),而未受影响的父母和兄弟姐妹中存在外显子,证实该突变在研究家族中分离为常染色体隐性突变。大缺失可能在mRNA水平上迫使外显子2与外显子6的连接,导致开放阅读框的移位。在200个不相关的内部对照中未观察到该突变。结论:据我们所知,这是第一个报道最大的巨型缺失,包括15.40 kb的移除(包括99个氨基酸),并揭示了BFSP1在生理晶状体功能和光学特性中的重要作用。我们的发现扩展了bfsp1相关白内障的基因型谱。
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