Pub Date : 2020-10-01DOI: 10.22038/NMJ.2020.07.00008
H. Vahidi, F. Kobarfard, Zahra Kosar, M. Mahjoub, M. Saravanan, H. Barabadi
Objective(s): This study deals with mycosynthesis and characterization of selenium nanoparticles (SeNPs) using the Penicillium chrysogenum PTCC 5031 and evaluating their antibacterial activity. Materials and Methods: The formation of SeNPs was confirmed with the color change from pale yellow to orange. Tyndall effect also confirmed the formation of colloidal systems through the samples. The SeNPs were characterized using different analytical techniques including photon correlation spectroscopy (PCS), Scanning Electron Microscope (SEM), Atomic Force Microscope (AFM), Energy Dispersive X-ray (EDX), X-ray diffraction (XRD) and Fourier Transform Infrared (FT-IR) analysis.Results: Our findings revealed that SeNPs were fairly uniformed with good monodispersity and the lesser aggregation of particles in pH value of 7 with the average hydrodynamic size of 24.65 nm, polydispersity index (PdI) of 0.392 and zeta potential of -34 mV. The SeNPs revealed antibacterial activity against gram positive bacteria including Staphylococcus aureus, and Listeria monocytogenes with the zone of inhibition (ZOI) of 10 and 13 mm, respectively. Conclusion: The results of this study provided a potential solution to the growing need for the development of cost-effective and eco-friendly ways of nanoparticle synthesis to overcome the microbial resistance and control the infectious diseases. However, further investigations are required to demonstrate the efficacy of SeNPs through in vivo models.
{"title":"Mycosynthesis and characterization of selenium nanoparticles using standard penicillium chrysogenum PTCC 5031 and their antibacterial activity: A novel approach in microbial nanotechnology","authors":"H. Vahidi, F. Kobarfard, Zahra Kosar, M. Mahjoub, M. Saravanan, H. Barabadi","doi":"10.22038/NMJ.2020.07.00008","DOIUrl":"https://doi.org/10.22038/NMJ.2020.07.00008","url":null,"abstract":"Objective(s): This study deals with mycosynthesis and characterization of selenium nanoparticles (SeNPs) using the Penicillium chrysogenum PTCC 5031 and evaluating their antibacterial activity. Materials and Methods: The formation of SeNPs was confirmed with the color change from pale yellow to orange. Tyndall effect also confirmed the formation of colloidal systems through the samples. The SeNPs were characterized using different analytical techniques including photon correlation spectroscopy (PCS), Scanning Electron Microscope (SEM), Atomic Force Microscope (AFM), Energy Dispersive X-ray (EDX), X-ray diffraction (XRD) and Fourier Transform Infrared (FT-IR) analysis.Results: Our findings revealed that SeNPs were fairly uniformed with good monodispersity and the lesser aggregation of particles in pH value of 7 with the average hydrodynamic size of 24.65 nm, polydispersity index (PdI) of 0.392 and zeta potential of -34 mV. The SeNPs revealed antibacterial activity against gram positive bacteria including Staphylococcus aureus, and Listeria monocytogenes with the zone of inhibition (ZOI) of 10 and 13 mm, respectively. Conclusion: The results of this study provided a potential solution to the growing need for the development of cost-effective and eco-friendly ways of nanoparticle synthesis to overcome the microbial resistance and control the infectious diseases. However, further investigations are required to demonstrate the efficacy of SeNPs through in vivo models.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":"7 1","pages":"315-323"},"PeriodicalIF":1.5,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46790077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-10-01DOI: 10.22038/NMJ.2020.07.00003
Karthigadevi Guruviah, S. Annamalai, Arulvel Ramaswamy, C. Sivasankaran, Subbaiya Ramasamy, H. Barabadi, M. Saravanan
Objective(s): This study was aimed to investigate the synthesis of novel zinc oxide (ZnO) nanoparticles (NPs) using Solanum trilobatum leaf extract as the reducing and capping agents, called green synthesized zinc oxide nanoparticles (GS-ZnONPs). Materials and Methods: Chemically synthesized zinc oxide nanoparticles (CS-ZnONPs) were synthesized using precipitation method with zinc nitrates hexahydrate as reducing precursors. The synthesized GS- and CS-ZnONPs were examined and characterized using UV-visible spectroscopy, Transmission Electron Microscopy (TEM), Scanning Electron microscopy (SEM), Energy dispersive X-ray analysis (EDAX), and X-ray diffraction (XRD) analysis, respectively. Results: GS-ZnONPs exhibited a higher zone of inhibition of 28.6 mm, 27.63 mm, and 29.33 mm for Bacillus subtilis, Escherichia coli, and Klebsiella pneumoniae, respectively compared to CS-ZnONPs. From the growth inhibition experiments with E. coli and Staphylococcus aureus, it was evident that GS-ZnONPs have exhibited higher growth inhibition as compared to CS-ZnONPs. The IC50 for CS-ZnONPs in MCF-7 cell line was found at 136.16 µg/mL and for GS-ZnONPs was found at 85.05 µg/mL. The proliferation of cancer cells were directly proportional to the concentration of NPs. As compared to CS-ZnONPs, GS-ZnONPs have exhibited higher cytotoxic effects on MCF-7 cell line. Conclusion: It was concluded that GS-ZnONPs represented much enhanced anticancer and antibacterial activity compared to CS-ZnONPs.
{"title":"Comparative antimicrobial and anticancer activity of biologically and chemically synthesized zinc oxide nanoparticles toward breast cancer cells","authors":"Karthigadevi Guruviah, S. Annamalai, Arulvel Ramaswamy, C. Sivasankaran, Subbaiya Ramasamy, H. Barabadi, M. Saravanan","doi":"10.22038/NMJ.2020.07.00003","DOIUrl":"https://doi.org/10.22038/NMJ.2020.07.00003","url":null,"abstract":"Objective(s): This study was aimed to investigate the synthesis of novel zinc oxide (ZnO) nanoparticles (NPs) using Solanum trilobatum leaf extract as the reducing and capping agents, called green synthesized zinc oxide nanoparticles (GS-ZnONPs). Materials and Methods: Chemically synthesized zinc oxide nanoparticles (CS-ZnONPs) were synthesized using precipitation method with zinc nitrates hexahydrate as reducing precursors. The synthesized GS- and CS-ZnONPs were examined and characterized using UV-visible spectroscopy, Transmission Electron Microscopy (TEM), Scanning Electron microscopy (SEM), Energy dispersive X-ray analysis (EDAX), and X-ray diffraction (XRD) analysis, respectively. Results: GS-ZnONPs exhibited a higher zone of inhibition of 28.6 mm, 27.63 mm, and 29.33 mm for Bacillus subtilis, Escherichia coli, and Klebsiella pneumoniae, respectively compared to CS-ZnONPs. From the growth inhibition experiments with E. coli and Staphylococcus aureus, it was evident that GS-ZnONPs have exhibited higher growth inhibition as compared to CS-ZnONPs. The IC50 for CS-ZnONPs in MCF-7 cell line was found at 136.16 µg/mL and for GS-ZnONPs was found at 85.05 µg/mL. The proliferation of cancer cells were directly proportional to the concentration of NPs. As compared to CS-ZnONPs, GS-ZnONPs have exhibited higher cytotoxic effects on MCF-7 cell line. Conclusion: It was concluded that GS-ZnONPs represented much enhanced anticancer and antibacterial activity compared to CS-ZnONPs.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":"7 1","pages":"272-283"},"PeriodicalIF":1.5,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42645707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-10-01DOI: 10.22038/NMJ.2020.07.00002
farnaz-sadat Fattahi, T. Zamani
The usage of clinical devices in the cardiovascular treatment, hemodialyze system and other biomedical applications has improved recently. Direct contacts of biomaterials like poly(lactic acid) biopolymer with blood result in the activating of platelets, white blood cells , coagulation structure and complement cascades. Poly(lactic acid) is a sustainable, renewable, compostable, biobased, biodegradable, bioabsorbable , biocompatible polymer. This polymer has many applications in the synthesis of blood contacting mats like nanofibrous vascular scaffolds and hemodialyze nanosheets.Mechanical interruption of the blood vessel wall throughout grafting of cardiovascular devices starts local hemostatic replies. Improving the safety of the blood contacting nanostructure grafts is a main necessity. The controlling of the interactions of proteins and platelets to the surface of a blood contacting biomaterial is a significant factor. So, the assessments of these material’s influences on blood are necessary.This article references more than 80 articles published in the last decade and reviews the latest hemocompatibility assays of poly(lactic acid) nanostructures used in the blood contacting field.
{"title":"Hemocompatibility poly (lactic acid) nanostructures: A bird’s eye view","authors":"farnaz-sadat Fattahi, T. Zamani","doi":"10.22038/NMJ.2020.07.00002","DOIUrl":"https://doi.org/10.22038/NMJ.2020.07.00002","url":null,"abstract":"The usage of clinical devices in the cardiovascular treatment, hemodialyze system and other biomedical applications has improved recently. Direct contacts of biomaterials like poly(lactic acid) biopolymer with blood result in the activating of platelets, white blood cells , coagulation structure and complement cascades. Poly(lactic acid) is a sustainable, renewable, compostable, biobased, biodegradable, bioabsorbable , biocompatible polymer. This polymer has many applications in the synthesis of blood contacting mats like nanofibrous vascular scaffolds and hemodialyze nanosheets.Mechanical interruption of the blood vessel wall throughout grafting of cardiovascular devices starts local hemostatic replies. Improving the safety of the blood contacting nanostructure grafts is a main necessity. The controlling of the interactions of proteins and platelets to the surface of a blood contacting biomaterial is a significant factor. So, the assessments of these material’s influences on blood are necessary.This article references more than 80 articles published in the last decade and reviews the latest hemocompatibility assays of poly(lactic acid) nanostructures used in the blood contacting field.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":"7 1","pages":"263-271"},"PeriodicalIF":1.5,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44117109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The usage of clinical devices in the cardiovascular treatment, hemodialyze system and other biomedical applications has improved recently. Direct contacts of biomaterials like poly(lactic acid) biopolymer with blood result in the activating of platelets, white blood cells , coagulation structure and complement cascades. Poly(lactic acid) is a sustainable, renewable, compostable, biobased, biodegradable, bioabsorbable , biocompatible polymer. This polymer has many applications in the synthesis of blood contacting mats like nanofibrous vascular scaffolds and hemodialyze nanosheets.Mechanical interruption of the blood vessel wall throughout grafting of cardiovascular devices starts local hemostatic replies. Improving the safety of the blood contacting nanostructure grafts is a main necessity. The controlling of the interactions of proteins and platelets to the surface of a blood contacting biomaterial is a significant factor. So, the assessments of these material’s influences on blood are necessary.This article references more than 80 articles published in the last decade and reviews the latest hemocompatibility assays of poly(lactic acid) nanostructures used in the blood contacting field.
{"title":"Hemocompatibility assays of poly (lactic acid) nanostructures: A bird’s eye view","authors":"farnaz-sadat Fattahi, T. Zamani","doi":"10.22038/NMJ.2020.16452","DOIUrl":"https://doi.org/10.22038/NMJ.2020.16452","url":null,"abstract":"The usage of clinical devices in the cardiovascular treatment, hemodialyze system and other biomedical applications has improved recently. Direct contacts of biomaterials like poly(lactic acid) biopolymer with blood result in the activating of platelets, white blood cells , coagulation structure and complement cascades. Poly(lactic acid) is a sustainable, renewable, compostable, biobased, biodegradable, bioabsorbable , biocompatible polymer. This polymer has many applications in the synthesis of blood contacting mats like nanofibrous vascular scaffolds and hemodialyze nanosheets.Mechanical interruption of the blood vessel wall throughout grafting of cardiovascular devices starts local hemostatic replies. Improving the safety of the blood contacting nanostructure grafts is a main necessity. The controlling of the interactions of proteins and platelets to the surface of a blood contacting biomaterial is a significant factor. So, the assessments of these material’s influences on blood are necessary.This article references more than 80 articles published in the last decade and reviews the latest hemocompatibility assays of poly(lactic acid) nanostructures used in the blood contacting field.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":"1 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2020-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45555358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-01DOI: 10.22038/NMJ.2020.07.0008
H. Ghorbani, A. H. Kasgari, P. Valipour, F. Jafari
Objective(s): A combination of biological and microemulsion methods was used to synthesize silver nanoparticles for the first time. The applied method could be referred to as the biomicroemulsion method, which has the advantages of both biological and the microemulsion methods.Materials and Methods: In the present study, silver nanoparticles were synthesized in a water-in-oil biomicroemulsion using silver nitrate, which was solubilized in the water core of one microemulsion as the source of silver ions. In addition, a bacterial culture supernatant solubilized in the water core of another microemulsion was employed as the biological reducing agent, dodecane was used as the oil phase, and sodium bis(2-ethylhexyl) sulfosuccinate was applied as the surfactant. Moreover, the antibacterial activity of the nanoparticles was investigated against gram-positive and gram-negative bacteria by disc-diffusion method.Results: The UV-Vis absorption spectra, dynamic light scattering, and transmission electron microscopy were employed to characterize the presence, size distribution, and morphology of the nanoparticles, respectively. According to the results, the nanoparticles had the optimal conditions in terms of the size and distribution at the silver nitrate concentration of 0.001 M. In addition, the analysis of antibacterial activity indicated that the inhibition zone diameter of Staphylococcus aureus was higher compared to Escherichia coli.Conclusion: Silver nanoparticles were synthesized successfully using biomicroemulsion method and showed significant anti-bacterial activities against S. aureus and E. coli.
{"title":"The synthesis of silver nanoparticles using the water-in-oil biomicroemulsion method","authors":"H. Ghorbani, A. H. Kasgari, P. Valipour, F. Jafari","doi":"10.22038/NMJ.2020.07.0008","DOIUrl":"https://doi.org/10.22038/NMJ.2020.07.0008","url":null,"abstract":"Objective(s): A combination of biological and microemulsion methods was used to synthesize silver nanoparticles for the first time. The applied method could be referred to as the biomicroemulsion method, which has the advantages of both biological and the microemulsion methods.Materials and Methods: In the present study, silver nanoparticles were synthesized in a water-in-oil biomicroemulsion using silver nitrate, which was solubilized in the water core of one microemulsion as the source of silver ions. In addition, a bacterial culture supernatant solubilized in the water core of another microemulsion was employed as the biological reducing agent, dodecane was used as the oil phase, and sodium bis(2-ethylhexyl) sulfosuccinate was applied as the surfactant. Moreover, the antibacterial activity of the nanoparticles was investigated against gram-positive and gram-negative bacteria by disc-diffusion method.Results: The UV-Vis absorption spectra, dynamic light scattering, and transmission electron microscopy were employed to characterize the presence, size distribution, and morphology of the nanoparticles, respectively. According to the results, the nanoparticles had the optimal conditions in terms of the size and distribution at the silver nitrate concentration of 0.001 M. In addition, the analysis of antibacterial activity indicated that the inhibition zone diameter of Staphylococcus aureus was higher compared to Escherichia coli.Conclusion: Silver nanoparticles were synthesized successfully using biomicroemulsion method and showed significant anti-bacterial activities against S. aureus and E. coli.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":"7 1","pages":"237-242"},"PeriodicalIF":1.5,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42290699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-01DOI: 10.22038/NMJ.2020.07.0005
Aditya N. Pandey, Kuldeep Rajpoot, Sunil K. Jain
Objective(s): Colorectal cancer (CRC) is a prevalent cancer worldwide. The present study aimed to synthesize and investigate the potential of wheat germ agglutinin (WGA) conjugated with polylactic-co-glycolic acid (PLGA) nanoparticles (NPs) incorporating 5-fluorouracil (5-FU). Materials and Methods: The NPs were investigated in terms of various characteristics, such as the particle size, surface charge, surface morphology, entrapment efficiency rate, and in-vitro drug release profile in simulated gastric and intestinal fluids. The optimized NPs were conjugated with WGA and characterized for the WGA conjugation efficiency, mucoadhesion, and cytotoxicity studies. Results: The zeta potential of the WGA-conjugated NPs decreased (-17.9±1.4 mV) possibly due to the conjugation of the NPs with WGA, which reduced the zeta potential. The WGA-conjugated NPs exhibited sustained drug release effects (p<0.05) compared to the marketed formulation containing 5-FU after 24 hours. In addition, the optimized NPs followed the Higuchi kinetics, showing diffusion-controlled drug release mechanisms. Finally, the WGA-conjugated PLGA NPs could significantly inhibit the growth of colon cancer cells (HT-29 and COLO-205) compared to the non-conjugated NPs and pure drug solution (P<0.05). Conclusion: According to the results, the WGA-conjugated NPs could be potential carrier systems compared to the non-conjugated NPs for the effective management of CRC.
{"title":"Using 5-fluorouracil-encored plga nanoparticles for the treatment of colorectal cancer: the in-vitro characterization and cytotoxicity studies","authors":"Aditya N. Pandey, Kuldeep Rajpoot, Sunil K. Jain","doi":"10.22038/NMJ.2020.07.0005","DOIUrl":"https://doi.org/10.22038/NMJ.2020.07.0005","url":null,"abstract":"Objective(s): Colorectal cancer (CRC) is a prevalent cancer worldwide. The present study aimed to synthesize and investigate the potential of wheat germ agglutinin (WGA) conjugated with polylactic-co-glycolic acid (PLGA) nanoparticles (NPs) incorporating 5-fluorouracil (5-FU). Materials and Methods: The NPs were investigated in terms of various characteristics, such as the particle size, surface charge, surface morphology, entrapment efficiency rate, and in-vitro drug release profile in simulated gastric and intestinal fluids. The optimized NPs were conjugated with WGA and characterized for the WGA conjugation efficiency, mucoadhesion, and cytotoxicity studies. Results: The zeta potential of the WGA-conjugated NPs decreased (-17.9±1.4 mV) possibly due to the conjugation of the NPs with WGA, which reduced the zeta potential. The WGA-conjugated NPs exhibited sustained drug release effects (p<0.05) compared to the marketed formulation containing 5-FU after 24 hours. In addition, the optimized NPs followed the Higuchi kinetics, showing diffusion-controlled drug release mechanisms. Finally, the WGA-conjugated PLGA NPs could significantly inhibit the growth of colon cancer cells (HT-29 and COLO-205) compared to the non-conjugated NPs and pure drug solution (P<0.05). Conclusion: According to the results, the WGA-conjugated NPs could be potential carrier systems compared to the non-conjugated NPs for the effective management of CRC.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":"7 1","pages":"211-224"},"PeriodicalIF":1.5,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47549951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-01DOI: 10.22038/NMJ.2020.07.0006
M. Mohammadi, M. Alibolandi, K. Abnous, Zahra Salmasi, M. Jaafari, M. Ramezani
Objective(s)Fabricating a biomimetic scaffold platform combined with controlled release of bioactive agents is a practical approach for bone tissue engineering. Controlled delivery of peptides and growth factors which play a significant role in osteogenesis is an important issue reducing the associated adverse effects and leading to cost-effectiveness.Materials and MethodsWe developed two liposomal formulations of bone morphogenetic protein-2 (BMP-2) peptide designated as F1 and F2 with controlled release properties. Due to high negative zeta potential of F1 formulation, the surface of the liposomes was decorated with positively charged BMP-2 peptide while the peptide was encapsulated in F2 formulation. Then, we evaluated the hypothesis that whether the electrostatically loaded peptide could act as a ligand and improve the cellular uptake and osteogenic differentiation of mesenchymal stem cells.ResultsBoth formulations were less than 100 nm in size. The release study revealed that both formulations showed a sustained release pattern for 21 days. However, the cumulative releases were 60% and 40% in F1 and F2 formulations, respectively. Flow cytometry analysis indicated that cell internalization of F1 liposomes was more than the other formulation. In the next step, F1 and F2 formulations were attached covalently to our previously developed nanofibrous electrospun scaffold and biocompatibility and osteogenic differentiation of each formulation were studied. The results indicated that the proliferation of the cells seeded on F1 liposcaffold was significantly more than F2 liposcaffold at days 1 and 3. Furthermore, F1 liposcaffold showed superior osteogenic differentiation through measurement of alkaline phosphatase activity which could be due to the higher release pattern of F1 liposomes and their improved cellular uptake.ConclusionOur findings revealed that controlled release BMP-2 decorated liposomal formulations immobilized on nanofibrous electrospun scaffold platform could be a promising candidate for bone regeneration therapeutics and merits further investigation.
{"title":"Comparison of liposomal formulations incorporating BMP-2 peptide to induce bone tissue engineering","authors":"M. Mohammadi, M. Alibolandi, K. Abnous, Zahra Salmasi, M. Jaafari, M. Ramezani","doi":"10.22038/NMJ.2020.07.0006","DOIUrl":"https://doi.org/10.22038/NMJ.2020.07.0006","url":null,"abstract":"Objective(s)Fabricating a biomimetic scaffold platform combined with controlled release of bioactive agents is a practical approach for bone tissue engineering. Controlled delivery of peptides and growth factors which play a significant role in osteogenesis is an important issue reducing the associated adverse effects and leading to cost-effectiveness.Materials and MethodsWe developed two liposomal formulations of bone morphogenetic protein-2 (BMP-2) peptide designated as F1 and F2 with controlled release properties. Due to high negative zeta potential of F1 formulation, the surface of the liposomes was decorated with positively charged BMP-2 peptide while the peptide was encapsulated in F2 formulation. Then, we evaluated the hypothesis that whether the electrostatically loaded peptide could act as a ligand and improve the cellular uptake and osteogenic differentiation of mesenchymal stem cells.ResultsBoth formulations were less than 100 nm in size. The release study revealed that both formulations showed a sustained release pattern for 21 days. However, the cumulative releases were 60% and 40% in F1 and F2 formulations, respectively. Flow cytometry analysis indicated that cell internalization of F1 liposomes was more than the other formulation. In the next step, F1 and F2 formulations were attached covalently to our previously developed nanofibrous electrospun scaffold and biocompatibility and osteogenic differentiation of each formulation were studied. The results indicated that the proliferation of the cells seeded on F1 liposcaffold was significantly more than F2 liposcaffold at days 1 and 3. Furthermore, F1 liposcaffold showed superior osteogenic differentiation through measurement of alkaline phosphatase activity which could be due to the higher release pattern of F1 liposomes and their improved cellular uptake.ConclusionOur findings revealed that controlled release BMP-2 decorated liposomal formulations immobilized on nanofibrous electrospun scaffold platform could be a promising candidate for bone regeneration therapeutics and merits further investigation.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":"7 1","pages":"225-230"},"PeriodicalIF":1.5,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49506323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-01DOI: 10.22038/NMJ.2020.07.0009
M. Ansari, H. Eslami
Objective(s) Artemisia absinthium is an aromatic, perennial small shrub that shows multiple medical benefits, including anticancerous, neuroprotective, antifungal, hepatoprotective, antidepressant and antioxidant properties. One of the effective approaches to treat Alzheimer’s disease is targeting amyloid aggregation by antiamyloid drugs. In the current research study, an excellent grouping of niosomal, lipid nano-carriers drugs containing artemisia absinthium is advanced and characterized to inhibit amyloid aggregation.Materials and Methods Niosomal vesicles were made employing phosphatidylcholine, span 60, cholesterol and DSPE-PEG2000 by the thin-film method. Then artemisia absinthium was loaded into the niosomes. Their physico-chemical attributes were analyzed utilizing Zeta-Sizer, FTIR, and SEM, and the amount of drug release was measured at 37° C. Finally, the inhibitory effect of artemisia absinthium that loaded niosomal vesicles on the aggregation of amyloid-β peptides was investigated using Thioflavin T fluorescence measurements and atomic force microscopy.Results Niosomes containing artemisia absinthium have a size of 174±2.56nm, the encapsulation efficiency of 66.73%, zeta potential of -26.5±1/42 mV and polydispersity index (PDI) of 0.373±0/02. The release of the drug is controlled in this nano-carrier and FTIR and SEM investigations showed that the drug and nano-carrier did not interact and their particles had a spherical structure. In the end, the inhibitory effect of artemisia absinthium that loaded niosomal vesicles on the aggregation of amyloid-β peptides was examined and confirmed through Thioflavin T fluorescence measurements and atomic force microscopy.Conclusion Meanwhile, the findings of the current study, confirm the appropriate physicochemical features of the system, a slow-release system, show that this nano-carrier inhibits amyloid aggregation, thus, the nano-niosomes containing essential oil from artemisia absinthium has the capability to preclude amyloid development.
{"title":"Preparation and study of the inhibitory effect of nano-niosomes containing essential oil from artemisia absinthium on amyloid fibril formation","authors":"M. Ansari, H. Eslami","doi":"10.22038/NMJ.2020.07.0009","DOIUrl":"https://doi.org/10.22038/NMJ.2020.07.0009","url":null,"abstract":"Objective(s) Artemisia absinthium is an aromatic, perennial small shrub that shows multiple medical benefits, including anticancerous, neuroprotective, antifungal, hepatoprotective, antidepressant and antioxidant properties. One of the effective approaches to treat Alzheimer’s disease is targeting amyloid aggregation by antiamyloid drugs. In the current research study, an excellent grouping of niosomal, lipid nano-carriers drugs containing artemisia absinthium is advanced and characterized to inhibit amyloid aggregation.Materials and Methods Niosomal vesicles were made employing phosphatidylcholine, span 60, cholesterol and DSPE-PEG2000 by the thin-film method. Then artemisia absinthium was loaded into the niosomes. Their physico-chemical attributes were analyzed utilizing Zeta-Sizer, FTIR, and SEM, and the amount of drug release was measured at 37° C. Finally, the inhibitory effect of artemisia absinthium that loaded niosomal vesicles on the aggregation of amyloid-β peptides was investigated using Thioflavin T fluorescence measurements and atomic force microscopy.Results Niosomes containing artemisia absinthium have a size of 174±2.56nm, the encapsulation efficiency of 66.73%, zeta potential of -26.5±1/42 mV and polydispersity index (PDI) of 0.373±0/02. The release of the drug is controlled in this nano-carrier and FTIR and SEM investigations showed that the drug and nano-carrier did not interact and their particles had a spherical structure. In the end, the inhibitory effect of artemisia absinthium that loaded niosomal vesicles on the aggregation of amyloid-β peptides was examined and confirmed through Thioflavin T fluorescence measurements and atomic force microscopy.Conclusion Meanwhile, the findings of the current study, confirm the appropriate physicochemical features of the system, a slow-release system, show that this nano-carrier inhibits amyloid aggregation, thus, the nano-niosomes containing essential oil from artemisia absinthium has the capability to preclude amyloid development.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":"7 1","pages":"243-250"},"PeriodicalIF":1.5,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41804853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-01DOI: 10.22038/NMJ.2020.07.0004
N. Akentieva, Аrthur R. Gizatullin, N. Sanina, N. Dremova, Vladimír, I. Torbov, N. Shkondina, N. Zhelev, S. Aldoshin
Objective(s)Currently, the development of nanoparticles for the stabilization and targeted delivery of cardiac drugs has gained significance. The present study aimed to develop nontoxic nanoparticles based on chitosan-hyaluronic acid (HA), encapsulate dinitrosyl iron complexes (DNICs, donors NO) into the nanoparticles to increase the stability and effectiveness of their action, and assess the effect of the nanoparticle-DNIC complex on the cell viability of cardiomyocytes.Materials and MethodsNanoparticles were obtained from chitosan-HA using the ionotropic gelation technology, and the morphology and size of the nanoparticles were determined using electron microscopy. The DNICs were built into the nanoparticles using the physical association method, and the stability of the nanoparticle-DNIC complexes and NO release was investigated using the electrochemical method.ResultsAnalysis by the electron microscopy showed that the nanoparticles were homogeneous in terms of shape and had an optimal size of ~100 nanometers. In addition, the incorporation of the DNICs into the composition of the nanoparticles significantly increased the stability of the DNICs, while also prolonging the generation of NO and enhancing the yield of nitrogen monoxide. Fluorescence analysis indicated that the chitosan-HA nanoparticles increased the cell viability of rat cardiomyocytes.ConclusionThe nanoparticles were fabricated from chitosan and HA. The encapsulation of the DNICs into the composition of the nanoparticles could stabilize these compounds, while prolonging and increasing the generated nitric oxide. The nanoparticle-DNICs were water-soluble, biocompatible, biodegradable, and nontoxic, which could be used as potential cardiac drugs for the treatment of cardiovascular diseases.
{"title":"Fabrication of chitosan-hyaluronic acid nanoparticles and encapsulation into nanoparticles of dinitrosyl iron complexes as potential cardiological drugs","authors":"N. Akentieva, Аrthur R. Gizatullin, N. Sanina, N. Dremova, Vladimír, I. Torbov, N. Shkondina, N. Zhelev, S. Aldoshin","doi":"10.22038/NMJ.2020.07.0004","DOIUrl":"https://doi.org/10.22038/NMJ.2020.07.0004","url":null,"abstract":"Objective(s)Currently, the development of nanoparticles for the stabilization and targeted delivery of cardiac drugs has gained significance. The present study aimed to develop nontoxic nanoparticles based on chitosan-hyaluronic acid (HA), encapsulate dinitrosyl iron complexes (DNICs, donors NO) into the nanoparticles to increase the stability and effectiveness of their action, and assess the effect of the nanoparticle-DNIC complex on the cell viability of cardiomyocytes.Materials and MethodsNanoparticles were obtained from chitosan-HA using the ionotropic gelation technology, and the morphology and size of the nanoparticles were determined using electron microscopy. The DNICs were built into the nanoparticles using the physical association method, and the stability of the nanoparticle-DNIC complexes and NO release was investigated using the electrochemical method.ResultsAnalysis by the electron microscopy showed that the nanoparticles were homogeneous in terms of shape and had an optimal size of ~100 nanometers. In addition, the incorporation of the DNICs into the composition of the nanoparticles significantly increased the stability of the DNICs, while also prolonging the generation of NO and enhancing the yield of nitrogen monoxide. Fluorescence analysis indicated that the chitosan-HA nanoparticles increased the cell viability of rat cardiomyocytes.ConclusionThe nanoparticles were fabricated from chitosan and HA. The encapsulation of the DNICs into the composition of the nanoparticles could stabilize these compounds, while prolonging and increasing the generated nitric oxide. The nanoparticle-DNICs were water-soluble, biocompatible, biodegradable, and nontoxic, which could be used as potential cardiac drugs for the treatment of cardiovascular diseases.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":"7 1","pages":"199-210"},"PeriodicalIF":1.5,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44774336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-01DOI: 10.22038/NMJ.2020.07.0002
Elnaz Bagheri, Legha Ansari, K. Abnous, S. M. Taghdisi, Mahshid Naserifar, M. Ramezani, M. Alibolandi
Several hybrid sensing materials, which are organized by interaction of organic molecules onto inorganic supports, have been developed as a novel and hopeful class of hybrid sensing probes. The hybrid silica-magnetic based sensors provide perfect properties for production of various devices in sensing technology. The hybridization of silica and magnetic NPs as biocompatible, biodegradable and superparamagnetic structures provides the opportunity to produce capable sensing materials. The fluorescence, electrochemical and calorimetric sensors based on silica-magnetic materials can be applied in quantitative detection of various analytes. This review touches upon a subject of the design and synthesis of different sensors based on magnetic-silica hybrid nanomaterials and discusses their applications for improved detection of analytes in environmental and biological fields.
{"title":"Silica -magnetic inorganic hybrid nanomaterials as versatile sensing platform","authors":"Elnaz Bagheri, Legha Ansari, K. Abnous, S. M. Taghdisi, Mahshid Naserifar, M. Ramezani, M. Alibolandi","doi":"10.22038/NMJ.2020.07.0002","DOIUrl":"https://doi.org/10.22038/NMJ.2020.07.0002","url":null,"abstract":"Several hybrid sensing materials, which are organized by interaction of organic molecules onto inorganic supports, have been developed as a novel and hopeful class of hybrid sensing probes. The hybrid silica-magnetic based sensors provide perfect properties for production of various devices in sensing technology. The hybridization of silica and magnetic NPs as biocompatible, biodegradable and superparamagnetic structures provides the opportunity to produce capable sensing materials. The fluorescence, electrochemical and calorimetric sensors based on silica-magnetic materials can be applied in quantitative detection of various analytes. This review touches upon a subject of the design and synthesis of different sensors based on magnetic-silica hybrid nanomaterials and discusses their applications for improved detection of analytes in environmental and biological fields.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":"7 1","pages":"183-193"},"PeriodicalIF":1.5,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48452947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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