Pub Date : 2013-08-01DOI: 10.1109/NANO.2013.6720883
Hui Tang, Yangmin Li, Yuchun Song
Recently, biomanipulation microrobots have attracted much attention in both academia and industry since they have become a promising tool to perform the practical biological micromanipulation tasks. In this field, biological micromanipulation on micro-fluidic chip emerge as a new focus since it can be used to improve the biomanipulation efficiency and stability. This study aims to develop a micromanipulation system with magnetic driven microrobots to implement the practical biomanipulations on a micro-fluidic chip. A novel flexure-based micromanipulator with large-workspace and micro/nano scale motion accuracy is proposed in this paper. After a series of mechanism optimal designs and analytic modeling, the positioning performance of the micromanipulators are evaluated by using the ANSYS Workbench Platform. After the mechanism fabrication, with the consideration of rate-dependent hysteresis effect inherent in piezoelectric ceramics (PZT) actuators, a novel hybrid visual servo control (HVSC) strategy combining with the Extreme Learning Machine (ELM) and the Artificial Neural Network (ANN) is proposed. Afterwards, a series of practical biomanipulation experiments are successfully implemented by using the designed control strategy. Both theoretical analysis and visual tracking results uniformly demonstrate the satisfactory performance of the developed system.
{"title":"Development and implementation of a biomanipulation system with magnetic-driven microrobots","authors":"Hui Tang, Yangmin Li, Yuchun Song","doi":"10.1109/NANO.2013.6720883","DOIUrl":"https://doi.org/10.1109/NANO.2013.6720883","url":null,"abstract":"Recently, biomanipulation microrobots have attracted much attention in both academia and industry since they have become a promising tool to perform the practical biological micromanipulation tasks. In this field, biological micromanipulation on micro-fluidic chip emerge as a new focus since it can be used to improve the biomanipulation efficiency and stability. This study aims to develop a micromanipulation system with magnetic driven microrobots to implement the practical biomanipulations on a micro-fluidic chip. A novel flexure-based micromanipulator with large-workspace and micro/nano scale motion accuracy is proposed in this paper. After a series of mechanism optimal designs and analytic modeling, the positioning performance of the micromanipulators are evaluated by using the ANSYS Workbench Platform. After the mechanism fabrication, with the consideration of rate-dependent hysteresis effect inherent in piezoelectric ceramics (PZT) actuators, a novel hybrid visual servo control (HVSC) strategy combining with the Extreme Learning Machine (ELM) and the Artificial Neural Network (ANN) is proposed. Afterwards, a series of practical biomanipulation experiments are successfully implemented by using the designed control strategy. Both theoretical analysis and visual tracking results uniformly demonstrate the satisfactory performance of the developed system.","PeriodicalId":189707,"journal":{"name":"2013 13th IEEE International Conference on Nanotechnology (IEEE-NANO 2013)","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123189064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We find that optical anisotropy of graphene films which play an important role as an alternative quality factor for the rapid characterization of large-area graphene films. Angle-variable spectroscopy method is developed to rapidly determine the optical anisotropy of graphene films. Unlike other approaches such like Raman scattering spectroscopy, this method allows ready, cheap, and large-area characterization of the structural qualities of CVD graphene films without the application of complicated optical setups.
{"title":"Rapidly characterize structural qualities of large-area graphene by optical anisotropy","authors":"Yu-Lun Liu, Hsuen‐Li Chen, Yu Chen-Chieh, Cheng-Yi Fang","doi":"10.1109/NANO.2013.6720914","DOIUrl":"https://doi.org/10.1109/NANO.2013.6720914","url":null,"abstract":"We find that optical anisotropy of graphene films which play an important role as an alternative quality factor for the rapid characterization of large-area graphene films. Angle-variable spectroscopy method is developed to rapidly determine the optical anisotropy of graphene films. Unlike other approaches such like Raman scattering spectroscopy, this method allows ready, cheap, and large-area characterization of the structural qualities of CVD graphene films without the application of complicated optical setups.","PeriodicalId":189707,"journal":{"name":"2013 13th IEEE International Conference on Nanotechnology (IEEE-NANO 2013)","volume":"104 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123378766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-08-01DOI: 10.1109/NANO.2013.6721054
Xiaocong Yang, Xiuxia Zhang, Yi Yang
Nano-diamond slurry was got by mixing with Nano-diamond powder, ethyl cellulose and solvent in a certain quality. Then the mixed solvent was dropped on ultra-white glass which fixed on sol-gel glue rejection machine. The machine was set to 3000 r/ min. After sintering, Nano-diamond film was got. Sintered glass was treated as the window layer of solar battery, and then conversion efficiency and impact factor were tested through IV characteristics system. As Nano-diamond have many excellent properties, such as the stability of chemical properties, resistance of high temperature and corrosion. So Nano-diamond film was chose as the window of solar cell because of. The different window layers of solar cells were contrasted and analyzed. And the result of Nano-diamond film's self-cleaning function could be got. What's more, the conversion efficiency of the solar cell could be improved.
{"title":"Research of nano-diamond film prepared on solar cell window","authors":"Xiaocong Yang, Xiuxia Zhang, Yi Yang","doi":"10.1109/NANO.2013.6721054","DOIUrl":"https://doi.org/10.1109/NANO.2013.6721054","url":null,"abstract":"Nano-diamond slurry was got by mixing with Nano-diamond powder, ethyl cellulose and solvent in a certain quality. Then the mixed solvent was dropped on ultra-white glass which fixed on sol-gel glue rejection machine. The machine was set to 3000 r/ min. After sintering, Nano-diamond film was got. Sintered glass was treated as the window layer of solar battery, and then conversion efficiency and impact factor were tested through IV characteristics system. As Nano-diamond have many excellent properties, such as the stability of chemical properties, resistance of high temperature and corrosion. So Nano-diamond film was chose as the window of solar cell because of. The different window layers of solar cells were contrasted and analyzed. And the result of Nano-diamond film's self-cleaning function could be got. What's more, the conversion efficiency of the solar cell could be improved.","PeriodicalId":189707,"journal":{"name":"2013 13th IEEE International Conference on Nanotechnology (IEEE-NANO 2013)","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121666915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-08-01DOI: 10.1109/NANO.2013.6720798
Yonghai Sun, J. Yeow, D. Jaffray
Ballast resistor under layer can improve reliability and stability of field emission cathode. Utilization of ballast resistor in Spindt cathode has been studied thoroughly. However, due to the significant difference between the Spindt cathode and CNT (carbon nanotube) emitter, studies of ballasted CNT emitter array are rare. Ballasted CNT emitter array has been demonstrated in our early studies. In this article, we use the finite element method to analyses the effect of the geometry parameters of the emission array. Optimized parameters, for example: intertube pitch, connection pad diameter, resistor layer thickness, and height of the CNT, are calculated. These optimized parameters are suitable for ballasted CNT emitter arrays for flat panel X-ray sources.
{"title":"Optimization of ballasted carbon nanotube array for X-ray source","authors":"Yonghai Sun, J. Yeow, D. Jaffray","doi":"10.1109/NANO.2013.6720798","DOIUrl":"https://doi.org/10.1109/NANO.2013.6720798","url":null,"abstract":"Ballast resistor under layer can improve reliability and stability of field emission cathode. Utilization of ballast resistor in Spindt cathode has been studied thoroughly. However, due to the significant difference between the Spindt cathode and CNT (carbon nanotube) emitter, studies of ballasted CNT emitter array are rare. Ballasted CNT emitter array has been demonstrated in our early studies. In this article, we use the finite element method to analyses the effect of the geometry parameters of the emission array. Optimized parameters, for example: intertube pitch, connection pad diameter, resistor layer thickness, and height of the CNT, are calculated. These optimized parameters are suitable for ballasted CNT emitter arrays for flat panel X-ray sources.","PeriodicalId":189707,"journal":{"name":"2013 13th IEEE International Conference on Nanotechnology (IEEE-NANO 2013)","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132458749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-08-01DOI: 10.1109/NANO.2013.6720830
Sarah J. Dempsey, A. Webb, A. Graham, D. Bloor, D. Atkinson, M. Szablewski
This paper presents an unusual conductive polymer composite, produced by Peratech Ltd under the trademark QTC™, which has many vapor sensing applications. Nickel particles are intimately coated by an elastomeric binder such that no percolative conduction can occur. However, the nickel particles are shown to possess spiky nanoscale surface features, which promote conduction by a field-assisted quantum tunneling mechanism. Granular QTC™ can be dispersed into a polymer matrix to produce a vapor sensor. Under exposure to vapor, the polymer swells and the resistance of the composite increases. In this work, granular sensors are subjected to acetone and tetrahydrofuran (THF) vapors. The response for THF shows an increase in resistance of a factor of 108, over a time-scale of a few seconds. This response is larger and faster than many conventional vapor sensing composites. This is a significantly larger response than that obtained historically for the same sensor, suggesting that some degree of sensor aging is desirable. The response and subsequent recovery can be explained by a case II diffusion model, and linked to Hildebrand solubility parameters of the vapor and polymer components.
{"title":"Vapor sensing properties of a conductive polymer composite containing Nickel particles with nano-scale surface features","authors":"Sarah J. Dempsey, A. Webb, A. Graham, D. Bloor, D. Atkinson, M. Szablewski","doi":"10.1109/NANO.2013.6720830","DOIUrl":"https://doi.org/10.1109/NANO.2013.6720830","url":null,"abstract":"This paper presents an unusual conductive polymer composite, produced by Peratech Ltd under the trademark QTC™, which has many vapor sensing applications. Nickel particles are intimately coated by an elastomeric binder such that no percolative conduction can occur. However, the nickel particles are shown to possess spiky nanoscale surface features, which promote conduction by a field-assisted quantum tunneling mechanism. Granular QTC™ can be dispersed into a polymer matrix to produce a vapor sensor. Under exposure to vapor, the polymer swells and the resistance of the composite increases. In this work, granular sensors are subjected to acetone and tetrahydrofuran (THF) vapors. The response for THF shows an increase in resistance of a factor of 108, over a time-scale of a few seconds. This response is larger and faster than many conventional vapor sensing composites. This is a significantly larger response than that obtained historically for the same sensor, suggesting that some degree of sensor aging is desirable. The response and subsequent recovery can be explained by a case II diffusion model, and linked to Hildebrand solubility parameters of the vapor and polymer components.","PeriodicalId":189707,"journal":{"name":"2013 13th IEEE International Conference on Nanotechnology (IEEE-NANO 2013)","volume":"405 30","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134289650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-08-01DOI: 10.1109/NANO.2013.6720955
Liangliang Chen, N. Xi, Ruiguo Yang, Bo Song, Zhanxin Zhou, Zhiyong Sun
Infrared (IR) sensors have been widely used for military and civil industry, while carbon nanotube (CNT) research progresses have been made in recent years. This led to investigation and development of CNT based IR detectors. In this paper, CNT-metal based Schottky barrier structure was designed to detect IR irradiance, and different interfacial layer, including SiO2, parylene-C, Si3N4, quartz, and polyimide, was used under CNT-metal contact to isolate substrate noise. The experimental results have shown that, the polymer based interfacial layer has strong substrate noise reduction on CNT IR sensor. The dark current is less than 0.3 nA under the condition of photocurrent 170 nA, noise at 0.5 nA when CNT-metal contact was fabricated on polyimide polymer due to substrate isolation and noise reduction.
{"title":"Substrate effect on single carbon nanotube based infrared sensors","authors":"Liangliang Chen, N. Xi, Ruiguo Yang, Bo Song, Zhanxin Zhou, Zhiyong Sun","doi":"10.1109/NANO.2013.6720955","DOIUrl":"https://doi.org/10.1109/NANO.2013.6720955","url":null,"abstract":"Infrared (IR) sensors have been widely used for military and civil industry, while carbon nanotube (CNT) research progresses have been made in recent years. This led to investigation and development of CNT based IR detectors. In this paper, CNT-metal based Schottky barrier structure was designed to detect IR irradiance, and different interfacial layer, including SiO2, parylene-C, Si3N4, quartz, and polyimide, was used under CNT-metal contact to isolate substrate noise. The experimental results have shown that, the polymer based interfacial layer has strong substrate noise reduction on CNT IR sensor. The dark current is less than 0.3 nA under the condition of photocurrent 170 nA, noise at 0.5 nA when CNT-metal contact was fabricated on polyimide polymer due to substrate isolation and noise reduction.","PeriodicalId":189707,"journal":{"name":"2013 13th IEEE International Conference on Nanotechnology (IEEE-NANO 2013)","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131893104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-08-01DOI: 10.1109/NANO.2013.6721008
H. Nguyen, B. Q. Ta, N. Hoivik, E. Halvorsen, K. Aasmundtveit
With increasing pressure on food industry to reduce waste while meeting the demand for high food quality and safety, there is a strong motivation for research into sensors for monitoring freshness and general food quality. Degradation of fresh meat and produce is related to metabolic activities of microorganisms which commonly result in formation of gaseous compounds such as CO2. Monitoring the freshness and quality of the food can therefore be accomplished by monitoring the release of these gases in the food package. In this study, an approach to create sensors for detecting gases released by perished food is described. Sensor test is conducted with CO2 which is a common gas related to the degradation process of food. The sensor is based on Carbon Nanotubes (CNTs) as sensing elements. A local synthesis technique is used to directly integrate the CNTs onto silicon-based circuits at room temperature. Fabricated CNT-based structures have successfully demonstrated the ability to detect CO2. The sensitivity and selectivity of gas sensors to different gas compounds were also studied. The results indicate that this approach is promising for fabrication of CNT-based gas sensors at low cost for determination of food freshness and quality, making possible complete gas sensors including CNT sensing elements, CMOS signal processing and RF communication on the same chip, manufactured and integrated at the wafer-level scale.
{"title":"Carbon nanotube based gas sensor for expiration detection of perishable food","authors":"H. Nguyen, B. Q. Ta, N. Hoivik, E. Halvorsen, K. Aasmundtveit","doi":"10.1109/NANO.2013.6721008","DOIUrl":"https://doi.org/10.1109/NANO.2013.6721008","url":null,"abstract":"With increasing pressure on food industry to reduce waste while meeting the demand for high food quality and safety, there is a strong motivation for research into sensors for monitoring freshness and general food quality. Degradation of fresh meat and produce is related to metabolic activities of microorganisms which commonly result in formation of gaseous compounds such as CO2. Monitoring the freshness and quality of the food can therefore be accomplished by monitoring the release of these gases in the food package. In this study, an approach to create sensors for detecting gases released by perished food is described. Sensor test is conducted with CO2 which is a common gas related to the degradation process of food. The sensor is based on Carbon Nanotubes (CNTs) as sensing elements. A local synthesis technique is used to directly integrate the CNTs onto silicon-based circuits at room temperature. Fabricated CNT-based structures have successfully demonstrated the ability to detect CO2. The sensitivity and selectivity of gas sensors to different gas compounds were also studied. The results indicate that this approach is promising for fabrication of CNT-based gas sensors at low cost for determination of food freshness and quality, making possible complete gas sensors including CNT sensing elements, CMOS signal processing and RF communication on the same chip, manufactured and integrated at the wafer-level scale.","PeriodicalId":189707,"journal":{"name":"2013 13th IEEE International Conference on Nanotechnology (IEEE-NANO 2013)","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132792950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-08-01DOI: 10.1109/NANO.2013.6720917
Long-Xian Ding, C. Durkan
Ferroelectric thin films have been intensively studied at the nanometre scale due to the application in many fields, such as non-volatile memories. Enhanced piezo-response force microscopy (E-PFM) was used to investigate the evolution of ferroelectric and ferroelastic nanodomains in a polycrystalline thin film of the simple multi-ferroic PbZr0.3Ti0.7O3 (PZT). By applying a d.c. voltage between the atomic force microscopy (AFM) tip and the bottom substrate of the sample, we created an electric field to switch the domain orientation. Reversible switching of both ferroelectric and ferroelastic domains towards particular directions with predominantly (111) domain orientations are observed. We also showed that along with the ferroelectric/ferroelastic domain switch, there are defects that also switch. Finally, we proposed the possible explanation of this controllable defect in terms of flexoelectricity and defect pinning.
{"title":"Controllable nanodomain defects in ferroelectric/ferroelastic biferroic thin films","authors":"Long-Xian Ding, C. Durkan","doi":"10.1109/NANO.2013.6720917","DOIUrl":"https://doi.org/10.1109/NANO.2013.6720917","url":null,"abstract":"Ferroelectric thin films have been intensively studied at the nanometre scale due to the application in many fields, such as non-volatile memories. Enhanced piezo-response force microscopy (E-PFM) was used to investigate the evolution of ferroelectric and ferroelastic nanodomains in a polycrystalline thin film of the simple multi-ferroic PbZr0.3Ti0.7O3 (PZT). By applying a d.c. voltage between the atomic force microscopy (AFM) tip and the bottom substrate of the sample, we created an electric field to switch the domain orientation. Reversible switching of both ferroelectric and ferroelastic domains towards particular directions with predominantly (111) domain orientations are observed. We also showed that along with the ferroelectric/ferroelastic domain switch, there are defects that also switch. Finally, we proposed the possible explanation of this controllable defect in terms of flexoelectricity and defect pinning.","PeriodicalId":189707,"journal":{"name":"2013 13th IEEE International Conference on Nanotechnology (IEEE-NANO 2013)","volume":"48 11","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133657224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-08-01DOI: 10.1109/NANO.2013.6720907
Jianjun Zhang, Xiaohui Wang, W. Liu, K. Qi, Weimin Li
Parallel micro-manipulators adopt flexible joints and the flexible deformation makes the up-platform move. Considering the counter-force/moment of the flexible joints, we concern the statics of a 6-PSS parallel micro-manipulator with perpendicular structures(PMMWPS), the construction of a relationship model between force output of PZT actuators and outer load on the up-platform, and the definition of driving stiffness matrix. As the particularity of the micro-manipulator, its stiffness is defined and stiffness matrix is derived. Finally, giving numerical value of the micro-manipulator parameters, we figure out the result that was verified by aid of Ansys software. The result of this paper is very useful for the design and dynamics analysis of the parallel micro-manipulator.
{"title":"On the study of statics and stiffness of a 6-PSS parallel micro-manipulator with perpendicular structures","authors":"Jianjun Zhang, Xiaohui Wang, W. Liu, K. Qi, Weimin Li","doi":"10.1109/NANO.2013.6720907","DOIUrl":"https://doi.org/10.1109/NANO.2013.6720907","url":null,"abstract":"Parallel micro-manipulators adopt flexible joints and the flexible deformation makes the up-platform move. Considering the counter-force/moment of the flexible joints, we concern the statics of a 6-PSS parallel micro-manipulator with perpendicular structures(PMMWPS), the construction of a relationship model between force output of PZT actuators and outer load on the up-platform, and the definition of driving stiffness matrix. As the particularity of the micro-manipulator, its stiffness is defined and stiffness matrix is derived. Finally, giving numerical value of the micro-manipulator parameters, we figure out the result that was verified by aid of Ansys software. The result of this paper is very useful for the design and dynamics analysis of the parallel micro-manipulator.","PeriodicalId":189707,"journal":{"name":"2013 13th IEEE International Conference on Nanotechnology (IEEE-NANO 2013)","volume":"67 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132762550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-08-01DOI: 10.1109/NANO.2013.6720846
W. Maneeprakorn, C. Apiwat, T. Dharakul A
A lateral flow colorimetric immunoassay (LFA) based on gold nanoparticles (AuNP) for detecting the antibody-antigen interaction and gold enhancement for amplifying the specific binding signal has been developed. Influenza A was used as the model study to determine the performance of the system. Nucleoprotein (NP) of the influaza A virus was selected as the targeting molecule. Two different sizes of AuNP conjugated to antibody against NP were prepared and applied to the assay. The 1st conjugate was AuNP immobilized with an anti-NP antibody and secondary-immobilized with biotinylated bovine serum albumin (B-BSA). The 2nd conjugate was AuNP immobilized with an anti-biotin antibody. The detection sensitivity of the enhanced sensitivity LFA increased about 3-folds and 8-folds compared with conventional LFA and commercial influenza A LFA, respectively. The signal intensity of the test spot/line at the capture test zone was increased dramatically. With this method, nucleoprotein of influenza A virus can be detected within 15 minutes and was highly reproducible. The results indicated that the technique can facilitate the more sensitive LFA test for diagnosis of many diseases.
{"title":"Enhancing sensitivity in colorimetric immunoassay by using secondary antibody-gold nanoparticle conjugate","authors":"W. Maneeprakorn, C. Apiwat, T. Dharakul A","doi":"10.1109/NANO.2013.6720846","DOIUrl":"https://doi.org/10.1109/NANO.2013.6720846","url":null,"abstract":"A lateral flow colorimetric immunoassay (LFA) based on gold nanoparticles (AuNP) for detecting the antibody-antigen interaction and gold enhancement for amplifying the specific binding signal has been developed. Influenza A was used as the model study to determine the performance of the system. Nucleoprotein (NP) of the influaza A virus was selected as the targeting molecule. Two different sizes of AuNP conjugated to antibody against NP were prepared and applied to the assay. The 1st conjugate was AuNP immobilized with an anti-NP antibody and secondary-immobilized with biotinylated bovine serum albumin (B-BSA). The 2nd conjugate was AuNP immobilized with an anti-biotin antibody. The detection sensitivity of the enhanced sensitivity LFA increased about 3-folds and 8-folds compared with conventional LFA and commercial influenza A LFA, respectively. The signal intensity of the test spot/line at the capture test zone was increased dramatically. With this method, nucleoprotein of influenza A virus can be detected within 15 minutes and was highly reproducible. The results indicated that the technique can facilitate the more sensitive LFA test for diagnosis of many diseases.","PeriodicalId":189707,"journal":{"name":"2013 13th IEEE International Conference on Nanotechnology (IEEE-NANO 2013)","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127823714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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