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IGF2BP2 inhibits invasion and migration of clear cell renal cell carcinoma via targeting Netrin-4 in an m6A-dependent manner. IGF2BP2 以 m6A 依赖性方式通过靶向 Netrin-4 抑制透明细胞肾细胞癌的侵袭和迁移。
IF 3 2区 医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-08-01 Epub Date: 2024-05-23 DOI: 10.1002/mc.23746
Gui Wang, Tao Zhuang, Fei Zhen, Chu Zhang, Qichao Wang, Xu Miao, Nienie Qi, Ruiqin Yao

Clear cell renal cell carcinoma (ccRCC), the most common subtype of renal cell carcinoma, often leads to a poor prognosis due to metastasis. The investigation of N6-methyladenosine (m6A) methylation, a crucial RNA modification, and its role in ccRCC, particularly through the m6A reader insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2), revealed significant insights. We found that IGF2BP2 was notably downregulated in ccRCC, which correlated with tumor aggressiveness and poor prognosis. Thus, IGFBP2 has emerged as an independent prognostic factor of ccRCC. Moreover, a strong positive correlation was observed between the expression of IGF2BP2 and Netrin-4. Netrin-4 was also downregulated in ccRCC, and its lower levels were associated with increased malignancy and poor prognosis. Overexpression of IGF2BP2 and Netrin-4 suppressed the invasion and migration of ccRCC cells, while Netrin-4 knockdown reversed these effects in ccRCC cell lines. RNA immunoprecipitation (RIP)-quantitative polymerase chain reaction validated the robust enrichment of Netrin-4 mRNA in anti-IGF2BP2 antibody immunoprecipitates. MeRlP showed significantly increased Netrin4 m6A levels after lGF2BP2 overexpression. Moreover, we found that IGF2BP2 recognized and bound to the m6A site within the coding sequence of Netrin-4, enhancing its mRNA stability. Collectively, these results showed that IGF2BP2 plays a suppressive role in the invasion and migration of ccRCC cells by targeting Netrin-4 in an m6A-dependent manner. These findings underscore the potential of IGF2BP2/Netrin-4 as a promising prognostic biomarker and therapeutic target in patients with ccRCC metastasis.

透明细胞肾细胞癌(ccRCC)是肾细胞癌中最常见的亚型,往往因转移而导致预后不良。N6-甲基腺苷(m6A)甲基化是一种关键的RNA修饰,研究它在ccRCC中的作用,特别是通过m6A读取胰岛素样生长因子2 mRNA结合蛋白2(IGF2BP2)的甲基化,揭示了重要的见解。我们发现,IGF2BP2 在 ccRCC 中明显下调,这与肿瘤的侵袭性和预后不良有关。因此,IGFBP2 已成为 ccRCC 的一个独立预后因素。此外,还观察到 IGF2BP2 的表达与 Netrin-4 之间存在很强的正相关性。Netrin-4也在ccRCC中下调,其水平较低与恶性程度增加和预后不良有关。在ccRCC细胞系中,过表达IGF2BP2和Netrin-4可抑制ccRCC细胞的侵袭和迁移,而Netrin-4的敲除可逆转这些效应。RNA免疫沉淀(RIP)-定量聚合酶链反应验证了抗IGF2BP2抗体免疫沉淀物中Netrin-4 mRNA的强力富集。MeRlP显示,lGF2BP2过表达后,Netrin4 m6A水平明显增加。此外,我们还发现 IGF2BP2 能识别并结合 Netrin-4 编码序列中的 m6A 位点,从而增强其 mRNA 的稳定性。总之,这些结果表明,IGF2BP2 通过以 m6A 依赖性方式靶向 Netrin-4 在 ccRCC 细胞的侵袭和迁移中起抑制作用。这些发现强调了IGF2BP2/Netrin-4作为ccRCC转移患者预后生物标志物和治疗靶点的潜力。
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引用次数: 0
LDHA-mediated M2-type macrophage polarization via tumor-derived exosomal EPHA2 promotes renal cell carcinoma progression. LDHA通过肿瘤外泌体EPHA2介导的M2型巨噬细胞极化促进了肾细胞癌的进展。
IF 3 2区 医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-08-01 Epub Date: 2024-05-23 DOI: 10.1002/mc.23737
Xinxin Gan, Jiatao Hu, Qingyang Pang, Rui Yan, Yi Bao, Ying Liu, Jiaao Song, Zheng Wang, Weihao Sun, Fuzhao Huang, Chen Cai, Linhui Wang

Lactate dehydrogenase A (LDHA) is known to promote the growth and invasion of various types of tumors, affects tumor resistance, and is associated with tumor immune escape. But how LDHA reshapes the tumor microenvironment and promotes the progression of renal cell carcinoma (RCC) remains unclear. In this study, we found that LDHA was highly expressed in clear cell RCC (ccRCC), and this high expression was associated with macrophage infiltration, while macrophages were highly infiltrated in ccRCC, affecting patient prognosis via M2-type polarization. Our in vivo and in vitro experiments demonstrated that LDHA and M2-type macrophages could enhance the proliferation, invasion, and migration abilities of ccRCC cells. Mechanistically, high expression of LDHA in ccRCC cells upregulated the expression of EPHA2 in exosomes derived from renal cancer. Exosomal EPHA2 promoted M2-type polarization of macrophages by promoting activation of the PI3K/AKT/mTOR pathway in macrophages, thereby promoting the progression of ccRCC. All these findings suggest that EPHA2 may prove to be a potential therapeutic target for advanced RCC.

众所周知,乳酸脱氢酶A(LDHA)可促进各类肿瘤的生长和侵袭,影响肿瘤的抗药性,并与肿瘤免疫逃逸有关。但LDHA如何重塑肿瘤微环境并促进肾细胞癌(RCC)的进展仍不清楚。在这项研究中,我们发现LDHA在透明细胞RCC(ccRCC)中高表达,这种高表达与巨噬细胞浸润有关,而巨噬细胞在ccRCC中高度浸润,通过M2型极化影响患者的预后。我们的体内和体外实验证明,LDHA和M2型巨噬细胞能增强ccRCC细胞的增殖、侵袭和迁移能力。从机理上讲,LDHA在ccRCC细胞中的高表达会上调肾癌外泌体中EPHA2的表达。外泌体EPHA2通过促进巨噬细胞中PI3K/AKT/mTOR通路的活化来促进巨噬细胞的M2型极化,从而促进ccRCC的进展。所有这些发现表明,EPHA2可能被证明是晚期RCC的潜在治疗靶点。
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引用次数: 0
Curcuminoid PBPD induces cuproptosis and endoplasmic reticulum stress in cervical cancer via the Notch1/RBP-J/NRF2/FDX1 pathway. 姜黄素PBPD通过Notch1/RBP-J/NRF2/FDX1途径诱导宫颈癌的杯突症和内质网应激反应
IF 3 2区 医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-08-01 Epub Date: 2024-05-27 DOI: 10.1002/mc.23735
Min-Jie Zhang, Mengna Shi, Yang Yu, Rongying Ou, Ren-Shan Ge, Ping Duan

Curcumin has been shown to have antitumor properties, but its low potency and bioavailability has limited its clinical application. We designed a novel curcuminoid, [1-propyl-3,5-bis(2-bromobenzylidene)-4-piperidinone] (PBPD), which has higher antitumor strength and improves bioavailability. Cell counting kit-8 was used to detect cell activity. Transwell assay was used to detect cell invasion and migration ability. Western blot and quantitative polymerase chain reaction were used to detect protein levels and their messenger RNA expression. Immunofluorescence was used to detect the protein location. PBPD significantly inhibited the proliferation of cervical cancer cells, with an IC50 value of 4.16 μM for Hela cells and 3.78 μM for SiHa cells, leading to the induction of cuproptosis. Transcriptome sequencing analysis revealed that PBPD significantly inhibited the Notch1/Recombination Signal Binding Protein for Immunoglobulin kappa J Region (RBP-J) and nuclear factor erythroid 2-related factor 2 (NRF2) signaling pathways while upregulating ferredoxin 1 (FDX1) expression. Knockdown of Notch1 or RBP-J significantly inhibited NRF2 expression and upregulated FDX1 expression, leading to the inhibition of nicotinamide adenine dinucleotide phosphate activity and the induction of oxidative stress, which in turn activated endoplasmic reticulum stress and induced cell death. The overexpression of Notch1 or RBP-J resulted in the enrichment of RBP-J within the NRF2 promoter region, thereby stimulating NRF2 transcription. NRF2 knockdown resulted in increase in FDX1 expression, leading to cuproptosis. In addition, PBPD inhibited the acidification of tumor niche and reduced cell metabolism to inhibit cervical cancer cell invasion and migration. In conclusion, PBPD significantly inhibits the proliferation, invasion, and migration of cervical cancer cells and may be a novel potential drug candidate for treatment of cervical cancer.

姜黄素已被证明具有抗肿瘤特性,但其低效力和低生物利用度限制了其临床应用。我们设计了一种新型姜黄素[1-丙基-3,5-双(2-溴亚苄基)-4-哌啶酮](PBPD),它具有更强的抗肿瘤能力并提高了生物利用度。细胞计数试剂盒-8 用于检测细胞活性。Transwell 试验用于检测细胞的侵袭和迁移能力。采用 Western 印迹和定量聚合酶链反应检测蛋白质水平及其信使 RNA 表达。免疫荧光用于检测蛋白质的位置。PBPD能明显抑制宫颈癌细胞的增殖,对Hela细胞的IC50值为4.16 μM,对SiHa细胞的IC50值为3.78 μM,从而诱导杯状突变。转录组测序分析表明,PBPD 能显著抑制 Notch1/Recombination Signal Binding Protein for Immunoglobulin kappa J Region (RBP-J) 和核因子红细胞 2 相关因子 2 (NRF2) 信号通路,同时上调铁毒素 1 (FDX1) 的表达。敲除Notch1或RBP-J可显著抑制NRF2的表达,上调FDX1的表达,从而抑制烟酰胺腺嘌呤二核苷酸磷酸酯的活性,诱导氧化应激,进而激活内质网应激,诱导细胞死亡。Notch1或RBP-J的过表达导致RBP-J在NRF2启动子区域富集,从而刺激NRF2的转录。敲除 NRF2 会导致 FDX1 表达增加,从而导致杯突。此外,PBPD 还能抑制肿瘤龛的酸化,降低细胞代谢,从而抑制宫颈癌细胞的侵袭和迁移。总之,PBPD 能显著抑制宫颈癌细胞的增殖、侵袭和迁移,可能是治疗宫颈癌的一种新型潜在候选药物。
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引用次数: 0
Multifaceted roles of PD-1 in tumorigenesis: From immune checkpoint to tumor cell-intrinsic function. PD-1 在肿瘤发生中的多重作用:从免疫检查点到肿瘤细胞内在功能
IF 3 2区 医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-08-01 Epub Date: 2024-05-15 DOI: 10.1002/mc.23740
Huiqing Chen, Jiayu Wei, Zhen Zhu, Yongzhong Hou

Programmed cell death 1 (PD-1), a key immune checkpoint receptor, has been extensively studied for its role in regulating immune responses in cancer. However, recent research has unveiled a complex and dual role for PD-1 in tumorigenesis. While PD-1 is traditionally associated with immune cells, this article explores its expression in various cancer cells and its impact on cancer progression. PD-1's functions extend beyond immune regulation, as it has been found to both promote and suppress tumor growth, depending on the cancer type. These findings have significant implications for the future of cancer treatment and our understanding of the immune response in the context of cancer. This article calls for further research into the multifaceted roles of PD-1 to optimize its therapeutic potential and improve patient outcomes in the fight against cancer.

程序性细胞死亡 1(PD-1)是一种关键的免疫检查点受体,它在调节癌症免疫反应方面的作用已被广泛研究。然而,最近的研究揭示了 PD-1 在肿瘤发生中的复杂和双重作用。虽然 PD-1 传统上与免疫细胞有关,但本文探讨了它在各种癌细胞中的表达及其对癌症进展的影响。PD-1 的功能超出了免疫调节的范畴,因为它既能促进肿瘤生长,也能抑制肿瘤生长,这取决于癌症的类型。这些发现对未来的癌症治疗以及我们对癌症免疫反应的理解具有重要意义。本文呼吁进一步研究 PD-1 的多方面作用,以优化其治疗潜力,改善患者的抗癌疗效。
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引用次数: 0
KNTC1 knockdown inhibits the proliferation and migration of osteosarcoma cells by MCM2. 敲除 KNTC1 可通过 MCM2 抑制骨肉瘤细胞的增殖和迁移。
IF 3 2区 医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-08-01 Epub Date: 2024-05-31 DOI: 10.1002/mc.23748
Lei Zhong, Yuanwei Dong, Shuqin Liu

Osteosarcoma (OS) is a common primary malignant bone tumor, and it is necessary to further investigate the molecular mechanism of OS progression. The expression of kinetochore associated protein 1 (KNTC1) and minichromosome maintenance 2 (MCM2) was detected by immunohistochemistry, quantitative PCR (qPCR) and Western blot. Gene knockdown or overexpression cell models were constructed and the proliferation, apoptosis, cell cycle and migration were detected in vitro, besides, xenograft models were established to explore the effects of KNTC1 downregulation in vivo. Public databased and bioinformatics analysis were performed to screen the downstream molecules and determine the expression of MCM2 in cancers. KNTC1 was overexpressed in OS tissues and positively correlated with overall survival of OS patients. KNTC1 knockdown inhibited the proliferation and migration, and arrested G2 phase, and induced apoptosis. Besides, KNTC1 downregulation restricted the xenograft tumor formation. MCM2, one of the coexpressed genes, was highly expressed in sarcoma and downregulated after KNTC1 knockdown. MCM2 overexpression heightened the proliferation and migration ability of OS cells, which was reversed the inhibiting effects of KNTC1 knockdown. KNTC1 was overexpressed in OS and promoted the progression of OS by upregulating MCM2.

骨肉瘤(Osteosarcoma,OS)是一种常见的原发性恶性骨肿瘤,有必要进一步研究OS进展的分子机制。本研究通过免疫组化、定量 PCR(qPCR)和 Western 印迹检测了动点相关蛋白 1(KNTC1)和迷你染色体维护 2(MCM2)的表达。此外,还建立了异种移植模型,以探讨 KNTC1 下调在体内的影响。通过公共数据库和生物信息学分析筛选下游分子,确定MCM2在癌症中的表达。KNTC1在OS组织中过表达,并与OS患者的总生存率呈正相关。敲除 KNTC1 可抑制细胞的增殖、迁移、G2 期停滞并诱导细胞凋亡。此外,KNTC1 的下调还限制了异种移植肿瘤的形成。MCM2是共表达基因之一,在肉瘤中高表达,在KNTC1敲除后下调。MCM2 的过表达增强了 OS 细胞的增殖和迁移能力,从而逆转了 KNTC1 的抑制作用。KNTC1在OS中过表达,并通过上调MCM2促进OS的进展。
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引用次数: 0
RETRACTION: "LncAPC Drives Wnt/Β-Catenin Activation and Liver TIC Self-Renewal Through EZH2 Mediated APC Transcriptional Inhibition". 返回:《LncAPC 通过 EZH2 介导的 APC 转录抑制驱动 Wnt/Β-Catenin 激活和肝脏 TIC 自我更新》。
IF 3 2区 医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-08-01 Epub Date: 2024-06-19 DOI: 10.1002/mc.23780

Retraction: X. Fu, J. Lin, F. Qin, Z. Yang, Y. Ding, Y. Zhang, L. Han, X. Zhu and Q. Zhang, "LncAPC Drives Wnt/Β-Catenin Activation and Liver TIC Self-Renewal Through EZH2 Mediated APC Transcriptional Inhibition," Molecular Carcinogenesis 57, no. 3 (2018): 408-418, https://doi.org/10.1002/mc.22764. The above article, published online on 16 November 2017 in Wiley Online Library (wileyonlinelibrary.com), and its correction, https://doi.org/10.1002/mc.23443, have been retracted by Wiley Periodicals, LLC. The retractions have been agreed following an investigation into concerns raised by a third party, which revealed inappropriate duplications of images with overlapping field of view between this and another article that was previously published in a different scientific context. Thus, the conclusions of this manuscript are substantially compromised. The authors and their institute were informed of the decision to retract but remained unresponsive.

撤回:X. Fu, J. Lin, F. Qin, Z. Yang, Y. Ding, Y. Zhang, L. Han, X. Zhu and Q. Zhang, "LncAPC Drives Wnt/Β-Catenin Activation and Liver TIC Self-Renewal Through EZH2 Mediated APC Transcriptional Inhibition," Molecular Carcinogenesis 57, no.3 (2018):408-418, https://doi.org/10.1002/mc.22764.2017年11月16日在线发表于Wiley Online Library(wileyonlinelibrary.com)的上述文章及其更正,https://doi.org/10.1002/mc.23443,已被Wiley Periodicals, LLC撤回。在对第三方提出的疑虑进行调查后,我们同意撤回上述稿件,因为调查显示,这篇文章与之前在不同科学背景下发表的另一篇文章之间存在视野重叠的不当重复图像。因此,本稿件的结论大打折扣。我们已将撤稿决定告知作者及其所在研究所,但他们仍未做出回应。
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引用次数: 0
The serine protease CORIN promotes progression of gastric cancer by mediating the ERK1/2 MAPK pathway. 丝氨酸蛋白酶 CORIN 通过介导 ERK1/2 MAPK 通路促进胃癌的进展。
IF 3 2区 医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-08-01 Epub Date: 2024-05-15 DOI: 10.1002/mc.23739
Runqi Hong, Xiaotian Zhang, Yi Zhang, Lanxin Bei, Ju Yang, Jie Xia, Zhiqing Hu, Zhipeng Cao, Rui Chen, Liang Chen, Gengming Niu, Chongwei Ke

The serine protease CORIN catalyzes pro-atrial natriuretic peptide (pro-ANP) into an active ANP and maintains homeostasis of the internal environment. However, it is unclear whether CORIN participates in the regulation of tumor progression. We analyzed the expression profile of CORIN in gastric cancer tissues (GCs) and adjacent nontumoral tissues (NTs). We investigated the prognostic value of CORIN in GC patients. We characterized the in vitro and in vivo activity of CORIN in cultured GC cells with gain-of-function and loss-of-function experiments. The underlying mechanism was explored by using bioinformatics, a signaling antibody array, and confirmative western blot analyses, as well as rescue experiments with highly selective small-molecule inhibitors targeting the ERK1/2 MAPK signaling pathway. CORIN was upregulated in GCs than in NTs. Overexpression of CORIN was correlated with unfavorable prognoses in patients with GC. Ectopic expression of CORIN was promoted, whereas silencing of CORIN suppressed proliferation, colony formation, migration and invasion of GC cells, and tumor growth in vivo. Overexpression of CORIN-induced epithelial-mesenchymal transition (EMT) and activation of the ERK1/2 MAPK signaling pathway, while silencing of CORIN yielded opposite results. The in vitro tumor-promoting potency of CORIN could be antagonized by selective inhibitors targeting the ERK1/2 MAPK pathway. In conclusion, CORIN is a potential prognostic marker and therapeutic target for GC patients, which may promote tumor progression by mediating the ERK1/2 MAPK signaling pathway and EMT in GC cells.

丝氨酸蛋白酶 CORIN 可将原心房钠尿肽(pro-ANP)催化成活性 ANP,并维持内环境的平衡。然而,CORIN 是否参与肿瘤进展的调控尚不清楚。我们分析了CORIN在胃癌组织(GCs)和邻近非肿瘤组织(NTs)中的表达谱。我们研究了 CORIN 在胃癌患者中的预后价值。我们通过功能增益和功能缺失实验鉴定了CORIN在培养的胃癌细胞中的体外和体内活性。我们利用生物信息学、信号传导抗体阵列、Western印迹确证分析以及针对ERK1/2 MAPK信号传导通路的高选择性小分子抑制剂进行了挽救实验,从而探索了其潜在机制。CORIN在GCs中比在NTs中上调。CORIN的过表达与GC患者的不良预后相关。异位表达CORIN可促进GC细胞的增殖、集落形成、迁移和侵袭以及体内肿瘤的生长,而沉默CORIN则可抑制GC细胞的增殖、集落形成、迁移和侵袭。过量表达CORIN会诱导上皮-间质转化(EMT)并激活ERK1/2 MAPK信号通路,而沉默CORIN则会产生相反的结果。针对ERK1/2 MAPK通路的选择性抑制剂可拮抗CORIN的体外肿瘤促进效力。总之,CORIN是GC患者的潜在预后标志物和治疗靶点,它可能通过介导ERK1/2 MAPK信号通路和GC细胞的EMT促进肿瘤进展。
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引用次数: 0
Alkaliptosis induction counteracts paclitaxel-resistant ovarian cancer cells via ATP6V0D1-mediated ABCB1 inhibition. 碱性诱导通过 ATP6V0D1 介导的 ABCB1 抑制作用抵消了卵巢癌细胞对紫杉醇的耐药性。
IF 3 2区 医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-08-01 Epub Date: 2024-05-15 DOI: 10.1002/mc.23741
Fangquan Chen, Junhao Lin, Rui Kang, Daolin Tang, Jiao Liu

Paclitaxel serves as the cornerstone chemotherapy for ovarian cancer, yet its prolonged administration frequently culminates in drug resistance, presenting a substantial challenge. Here we reported that inducing alkaliptosis, rather than apoptosis or ferroptosis, effectively overcomes paclitaxel resistance. Mechanistically, ATPase H+ transporting V0 subunit D1 (ATP6V0D1), a key regulator of alkaliptosis, plays a pivotal role by mediating the downregulation of ATP-binding cassette subfamily B member 1 (ABCB1), a multidrug resistance protein. Both ATP6V0D1 overexpression through gene transfection and pharmacological enhancement of ATP6V0D1 protein stability using JTC801 effectively inhibit ABCB1 upregulation, resulting in growth inhibition in drug-resistant cells. Additionally, increasing intracellular pH to alkaline (pH 8.5) via sodium hydroxide application suppresses ABCB1 expression, whereas reducing the pH to acidic conditions (pH 6.5) with hydrochloric acid amplifies ABCB1 expression in drug-resistant cells. Collectively, these results indicate a potentially effective therapeutic strategy for targeting paclitaxel-resistant ovarian cancer by inducing ATP6V0D1-dependent alkaliptosis.

紫杉醇是卵巢癌的基础化疗药物,但长期用药往往会产生耐药性,这是一个巨大的挑战。在这里,我们报告了诱导碱中毒,而不是诱导凋亡或铁中毒,可有效克服紫杉醇耐药性。从机理上讲,ATP酶H+转运V0亚基D1(ATP6V0D1)是碱中毒的关键调控因子,它通过介导多药耐药蛋白ATP结合盒B亚家族成员1(ABCB1)的下调而发挥关键作用。通过基因转染过量表达 ATP6V0D1 和使用 JTC801 通过药物增强 ATP6V0D1 蛋白的稳定性都能有效抑制 ABCB1 的上调,从而抑制耐药细胞的生长。此外,通过氢氧化钠将细胞内 pH 值升至碱性(pH 值 8.5)可抑制 ABCB1 的表达,而用盐酸将 pH 值降至酸性(pH 值 6.5)则可扩大耐药细胞中 ABCB1 的表达。总之,这些结果表明,通过诱导 ATP6V0D1 依赖性碱中毒,可以针对紫杉醇耐药的卵巢癌采取一种潜在有效的治疗策略。
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引用次数: 0
Upregulation of collagen type X alpha 1 promotes the progress of triple-negative breast cancer via Wnt/β-catenin signaling. 胶原蛋白X型α1的上调通过Wnt/β-catenin信号转导促进三阴性乳腺癌的进展。
IF 3 2区 医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-08-01 Epub Date: 2024-05-23 DOI: 10.1002/mc.23747
Jing Peng, Xiangping Liu, Yan Mao, Meng Lv, Teng Ma, Jiaxiu Liu, Quan Zhou, Yafei Han, Xin Li, Haibo Wang

Triple-negative breast cancer (TNBC) is a malignant tumor with high degree of malignancy and lack of effective target treatment. The research aims to explore the role and mechanism of X collagen alpha-1 chain protein (COL10A1 gene) in TNBC. UALCAN and Kaplan-Meier were used to detect the expression of COL10A1 and its role in the prognosis of breast cancer patients. The cells with stably expressing high levels of COL10A1 were obtained by recombinant lentivirus infection. The expression of COL10A1 in cells was temporarily downregulated by siRNA interference fragments. Real-time quantitative polymerase chain reaction and western blot analysis were utilized to detect the changes of COL10A1 mRNA and protein expression. The biological functions of the cells were evaluated by colony formation, cell counting kit-8, cell invasion and wound healing experiments. In addition, the effect of COL10A1 on angiogenesis was investigated by tube formation assay. Xenograft tumor model was used to confirm the effect of COL10A1 on tumorigenicity in vivo and multiplex fluorescent immunohistochemistry to detect multiple proteins simultaneously. The possible molecular mechanism of the function of COL10A1 was speculated through the detection of proteins in functionally related pathways. COL10A1 is highly expressed and is significantly associated with worse overall survival (OS) and recurrence-free survival (RFS) in TNBC. Overexpression of COL10A1 increased the clone formation rate and cell migration capacity of TNBC cells. In the COL10A1 overexpression group, the clone formation rates of MD-MB-231 and BT-549 cells (21.5 ± 0.62, 27.83 ± 3.72)% were significantly higher than those in the control group(15.23 ± 2.79, 19.4 ± 1.47)%, and the relative migration ratio (47.40 ± 3.09, 41.26 ± 4.33)% were higher than those in the control group (34.48 ± 2.03, 21.80 ± 1.03)%. When the expression of COL10A1 was downregulated, the ability of clone formation and wound-healing migration capacity in TNBC cells was weakened. Upregulated COL10A1 in TNBC cells generated more junctions and longer total segments between vascular endothelial cells, and promoted angiogenesis of the cells, and thus enhanced the tumorigenesis. In TNBC, it was found that COL10A1 might affect epithelial-mesenchymal transition (EMT) of the cells through Wnt/β-catenin signaling pathway by the detection of the related pathway proteins. COL10A1 is highly expressed in TNBC, and its high expression leads to poor OS and RFS. COL10A1 may enhance TNBC cell proliferation, migration and tumor-related angiogenesis, and promote tumorigenesis in vivo via Wnt/β-catenin signaling.

三阴性乳腺癌(TNBC)是一种恶性程度高且缺乏有效靶向治疗的恶性肿瘤。本研究旨在探讨X胶原蛋白α-1链蛋白(COL10A1基因)在TNBC中的作用和机制。研究采用 UALCAN 和 Kaplan-Meier 方法检测 COL10A1 的表达及其在乳腺癌患者预后中的作用。通过重组慢病毒感染获得稳定表达高水平COL10A1的细胞。通过 siRNA 干扰片段暂时下调细胞中 COL10A1 的表达。利用实时定量聚合酶链反应和 Western 印迹分析检测 COL10A1 mRNA 和蛋白表达的变化。通过集落形成、细胞计数试剂盒-8、细胞侵袭和伤口愈合实验评估了细胞的生物学功能。此外,还通过试管形成实验研究了 COL10A1 对血管生成的影响。利用异种移植肿瘤模型证实了 COL10A1 对体内致瘤性的影响,并利用多重荧光免疫组化技术同时检测了多种蛋白。通过检测功能相关通路中的蛋白,推测了COL10A1功能的可能分子机制。COL10A1的高表达与TNBC患者较差的总生存期(OS)和无复发生存期(RFS)显著相关。COL10A1的过表达增加了TNBC细胞的克隆形成率和细胞迁移能力。在COL10A1过表达组,MD-MB-231和BT-549细胞的克隆形成率(21.5±0.62,27.83±3.72)%明显高于对照组(15.23±2.79,19.4±1.47)%,相对迁移率(47.40±3.09,41.26±4.33)%高于对照组(34.48±2.03,21.80±1.03)%。当 COL10A1 表达下调时,TNBC 细胞的克隆形成能力和伤口愈合迁移能力减弱。COL10A1在TNBC细胞中上调后,血管内皮细胞之间的连接更多,总段更长,促进了细胞的血管生成,从而增强了肿瘤的发生。在TNBC中,通过检测相关通路蛋白发现,COL10A1可能通过Wnt/β-catenin信号通路影响细胞的上皮-间质转化(EMT)。COL10A1在TNBC中高表达,其高表达导致不良的OS和RFS。COL10A1可能通过Wnt/β-catenin信号增强TNBC细胞的增殖、迁移和肿瘤相关的血管生成,并在体内促进肿瘤发生。
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引用次数: 0
HER3 V104 mutations regulate cell signaling, growth, and drug sensitivity in cancer. HER3 V104 突变调节癌症的细胞信号、生长和药物敏感性。
IF 3 2区 医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-08-01 Epub Date: 2024-05-15 DOI: 10.1002/mc.23743
Rosalin Mishra, Mary Kate Kilroy, Wasim Feroz, Hima Patel, Joan T Garrett

HER3 is mutated in ~2%-10% of cancers depending on the cancer type. We found the HER3-V104L mutation to be activating from patient-derived mutations introduced via lentiviral transduction in HER3KO HER2 + HCC1569 breast cancer cells in which endogenous HER3 was eliminated by CRISPR/Cas9. Cells expressing HER3-V104L showed higher p-HER3 and p-ERK1/2 expression versus cells expressing wild-type HER3 or HER3-V104M. Patients whose tumor expressed the HER3 V104L variant had a reduced probability of overall survival compared to patients lacking a HER3 mutation whereas we did not find a statistically significant difference in overall survival of various cancer patients with the HER3 V104M mutation. Our data showed that HER2 inhibitors suppressed cell growth of HCC1569HER3KO cells stably expressing the HER3-V104L mutation. Cancer cell lines (SNU407, UC15 and DV90) with endogenous HER3-V104M mutation showed reduced cell proliferation and p-HER2/p-ERK1/2 expression with HER2 inhibitor treatment. Knock down of HER3 abrogated cell proliferation in the above cell lines which were overall more sensitive to the ERK inhibitor SCH779284 versus PI3K inhibitors. HER3-V104L mutation stabilized HER3 protein expression in COS7 and SNUC5 cells. COS7 cells transiently transfected with the HER3-V104L mutation in the presence of HER binding partners showed higher expression of p-HER3, p-ERK1/2 versus HER3-WT in a NRG-independent manner without any change in AKT signaling. Overall, this study shows the clinical relevance of the HER3 V104L and the V104M mutations and its response to HER2, PI3K and ERK inhibitors.

根据癌症类型的不同,约有 2%-10% 的癌症发生 HER3 突变。我们发现,在通过 CRISPR/Cas9 消除内源性 HER3 的 HER3KO HER2 + HCC1569 乳腺癌细胞中,通过慢病毒转导引入的患者来源突变可激活 HER3-V104L 突变。与表达野生型 HER3 或 HER3-V104M 的细胞相比,表达 HER3-V104L 的细胞显示出更高的 p-HER3 和 p-ERK1/2 表达。与没有HER3突变的患者相比,肿瘤表达HER3 V104L变体的患者的总生存概率较低,而我们没有发现HER3 V104M突变的各种癌症患者的总生存率有显著的统计学差异。我们的数据显示,HER2抑制剂抑制了稳定表达HER3-V104L突变的HCC1569HER3KO细胞的生长。内源性HER3-V104M突变的癌细胞株(SNU407、UC15和DV90)在接受HER2抑制剂治疗后,细胞增殖和p-HER2/p-ERK1/2表达均有所下降。敲除 HER3 可抑制上述细胞株的细胞增殖,总体而言,这些细胞株对 ERK 抑制剂 SCH779284 比对 PI3K 抑制剂更敏感。HER3-V104L 突变稳定了 COS7 和 SNUC5 细胞中 HER3 蛋白的表达。在 HER 结合伴侣存在的情况下,瞬时转染了 HER3-V104L 突变基因的 COS7 细胞与 HER3-WT 细胞相比,p-HER3、p-ERK1/2 的表达量更高,且不依赖于 NRG,AKT 信号转导没有发生任何变化。总之,这项研究表明了HER3 V104L和V104M突变的临床意义及其对HER2、PI3K和ERK抑制剂的反应。
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Molecular Carcinogenesis
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