Pub Date : 2024-01-12DOI: 10.1007/s11557-023-01942-0
S. Ullah, N. Afshan, A. Ishaq, Maria Riaz, M. Fiaz, Abdul Nasir Khalid, M. C. Aime
{"title":"Notes on species of Sporisorium and Ustilago from Pakistan","authors":"S. Ullah, N. Afshan, A. Ishaq, Maria Riaz, M. Fiaz, Abdul Nasir Khalid, M. C. Aime","doi":"10.1007/s11557-023-01942-0","DOIUrl":"https://doi.org/10.1007/s11557-023-01942-0","url":null,"abstract":"","PeriodicalId":19111,"journal":{"name":"Mycological Progress","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139437864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-29DOI: 10.1007/s11557-023-01940-2
Marjorie Cedeño-Sanchez, Tian Cheng, Christopher Lambert, Miroslav Kolarík, Eric Kuhnert, Russell J. Cox, Jörn Kalinowski, Bart Verwaaijen, Marc Stadler
Sequencing ribosomal DNA loci, such as ITS and LSU, are among the first steps to characterize and identify newly isolated fungal strains. Substantial doubt has recently been raised concerning the unequivocal suitability of using this sequence information for inferring inter- and intraspecific relationships, as especially the ITS locus was repeatedly shown to harbor high intragenomic plasticity. The evolutionary mechanisms leading to this phenomenon are poorly understood, but this is about to change, owing to the availability of third generation sequencing techniques. The current study attempts a comprehensive analysis of rDNA cistrons in the genomes of over 40 selected species of Hypoxylaceae (Xylariales), using a combination of 2nd generation (Illumina) and 3rd generation sequencing technologies (Oxford Nanopore), along with extensive usage of bioinformatic tools to obtain high-quality genomes. Our findings revealed high intragenomic polymorphisms in distinct Hypoxylaceae species, indicating the presence of deep rDNA paralogs. Phylogenetic inference of the ITS rDNA showed low support for the primary clades within Hypoxylaceae, while inference using the protein encoding gene RPB2 showed better support. We also found that proteinogenic genes are not prone to intraspecific and intragenomic polymorphisms as much as the rDNA loci. Our study highlights the limitations of solely relying on the rDNA cistron as a barcode for fungi. We propose TUB2 as a new primary barcoding marker for Hypoxylaceae and other Xylariales. Moreover, we demonstrate the feasibility to retrieve some DNA loci that had hitherto not been used for classical phylogenetic studies (such as TEF1) in Xylariales directly from genomes without the necessity to work with primers.
对 ITS 和 LSU 等核糖体 DNA 位点进行测序，是鉴定和识别新分离真菌菌株的第一步。最近，人们对利用这些序列信息推断种间和种内关系的明确适宜性提出了大量质疑，因为 ITS 基因座被反复证明具有高度的基因组内可塑性。人们对导致这种现象的进化机制知之甚少，但由于第三代测序技术的出现，这种情况即将得到改变。目前的研究采用第二代测序技术（Illumina）和第三代测序技术（Oxford Nanopore）相结合的方法，并广泛使用生物信息学工具来获得高质量的基因组，尝试对 40 多个精选的木犀科（Xylariales）物种基因组中的 rDNA cistrons 进行全面分析。我们的研究结果表明，在不同的hypoxylaceae物种中存在较高的基因组内多态性，这表明存在深层的rDNA旁系。ITS rDNA的系统发育推断结果表明，次黄嘌呤科内主要支系的支持率较低，而使用蛋白质编码基因RPB2进行的推断则显示出较好的支持率。我们还发现，蛋白基因不像 rDNA 位点那样容易发生种内和基因组内多态性。我们的研究强调了仅仅依靠 rDNA 单链子作为真菌条形码的局限性。我们建议将 TUB2 作为次黄嘌呤科和其他木霉菌的新的主要条形码标记。此外，我们还证明了直接从基因组中检索木霉菌中一些迄今为止尚未用于经典系统发育研究的 DNA 位点（如 TEF1）的可行性，而无需使用引物。
{"title":"Unraveling intragenomic polymorphisms in the high-quality genome of Hypoxylaceae: a comprehensive study of the rDNA cistron","authors":"Marjorie Cedeño-Sanchez, Tian Cheng, Christopher Lambert, Miroslav Kolarík, Eric Kuhnert, Russell J. Cox, Jörn Kalinowski, Bart Verwaaijen, Marc Stadler","doi":"10.1007/s11557-023-01940-2","DOIUrl":"https://doi.org/10.1007/s11557-023-01940-2","url":null,"abstract":"<p>Sequencing ribosomal DNA loci, such as ITS and LSU, are among the first steps to characterize and identify newly isolated fungal strains. Substantial doubt has recently been raised concerning the unequivocal suitability of using this sequence information for inferring inter- and intraspecific relationships, as especially the ITS locus was repeatedly shown to harbor high intragenomic plasticity. The evolutionary mechanisms leading to this phenomenon are poorly understood, but this is about to change, owing to the availability of third generation sequencing techniques. The current study attempts a comprehensive analysis of rDNA cistrons in the genomes of over 40 selected species of <i>Hypoxylaceae</i> (<i>Xylariales</i>), using a combination of 2nd generation (Illumina) and 3rd generation sequencing technologies (Oxford Nanopore), along with extensive usage of bioinformatic tools to obtain high-quality genomes. Our findings revealed high intragenomic polymorphisms in distinct <i>Hypoxylaceae</i> species, indicating the presence of deep rDNA paralogs. Phylogenetic inference of the ITS rDNA showed low support for the primary clades within <i>Hypoxylaceae</i>, while inference using the protein encoding gene <i>RPB2</i> showed better support. We also found that proteinogenic genes are not prone to intraspecific and intragenomic polymorphisms as much as the rDNA loci. Our study highlights the limitations of solely relying on the rDNA cistron as a barcode for fungi. We propose <i>TUB2</i> as a new primary barcoding marker for <i>Hypoxylaceae</i> and other <i>Xylariales</i>. Moreover, we demonstrate the feasibility to retrieve some DNA loci that had hitherto not been used for classical phylogenetic studies (such as <i>TEF1</i>) in <i>Xylariales</i> directly from genomes without the necessity to work with primers.</p>","PeriodicalId":19111,"journal":{"name":"Mycological Progress","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139070519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-22DOI: 10.1007/s11557-023-01941-1
Winnie Chemutai Sum, Didsanutda Gonkhom, Mahmoud A. A. Ibrahim, Marc Stadler, Sherif S. Ebada
Preparative high-performance liquid chromatography (HPLC) purification of the ethyl acetate extract derived from dried basidiomes of the European mushroom Hericium coralloides led to the identification of two previously undescribed isoindolinone derivatives named corallocins D and E (1-2). The structures of the compounds were elucidated based on HR-ESI-MS (high-resolution electron spray ionization mass spectrometry), interpretation of 1D and 2D NMR spectra, electronic circular dichroism (ECD) experiments, and comparisons with published and theoretical data. The metabolites were tested for their cytotoxic and antimicrobial effects in vitro where weak to moderate biological effects were observed against HeLa cells (KB 3.1), Mucor hiemalis and Bacillus subtilis.
通过对从欧洲蘑菇珊瑚菇（Hericium coralloides）干燥基生菌中提取的乙酸乙酯提取物进行制备型高效液相色谱（HPLC）纯化，发现了两种以前未曾描述过的异吲哚啉酮衍生物，分别被命名为珊瑚菇素 D 和 E（1-2）。根据 HR-ESI-MS（高分辨率电子喷雾电离质谱）、一维和二维核磁共振光谱解读、电子圆二色性（ECD）实验以及与已发表数据和理论数据的比较，阐明了这两种化合物的结构。对代谢物的细胞毒性和抗菌效果进行了体外测试，观察到它们对 HeLa 细胞（KB 3.1）、Mucor hiemalis 和枯草芽孢杆菌有弱至中等的生物效应。
{"title":"New isoindolinone derivatives isolated from the fruiting bodies of the basidiomycete Hericium coralloides","authors":"Winnie Chemutai Sum, Didsanutda Gonkhom, Mahmoud A. A. Ibrahim, Marc Stadler, Sherif S. Ebada","doi":"10.1007/s11557-023-01941-1","DOIUrl":"https://doi.org/10.1007/s11557-023-01941-1","url":null,"abstract":"<p>Preparative high-performance liquid chromatography (HPLC) purification of the ethyl acetate extract derived from dried basidiomes of the European mushroom <i>Hericium coralloides</i> led to the identification of two previously undescribed isoindolinone derivatives named corallocins D and E (<b>1</b>-<b>2</b>). The structures of the compounds were elucidated based on HR-ESI-MS (high-resolution electron spray ionization mass spectrometry), interpretation of 1D and 2D NMR spectra, electronic circular dichroism (ECD) experiments, and comparisons with published and theoretical data. The metabolites were tested for their cytotoxic and antimicrobial effects <i>in vitro</i> where weak to moderate biological effects were observed against HeLa cells (KB 3.1), <i>Mucor hiemalis</i> and <i>Bacillus subtilis</i>.</p>","PeriodicalId":19111,"journal":{"name":"Mycological Progress","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139030206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nacobbus aberrans is a plant parasitic nematode that causes significant economic losses in the American Continent and is considered a quarantine pest in many countries. Some arbuscular mycorrhizal fungi have shown the ability to reduce the population of this pathogen; however, most studies do not consider other relevant aspects that have to do with the crop or the fungus. The purpose of this work was to select an isolated mycorrhizal fungus based on a comprehensive analysis of the Nematode–Plant–Mycorrhizal Fungus system. The test was conducted on Solanum lycopersicum L. grown in pots. The evaluated isolates were Funneliformis mosseae, Rhizoglomus intraradices A2, and Rhizoglomus intraradices B1. All the isolates reduced the nematode population significantly; however, differences in the content of photosynthetic pigments, soluble proteins, and osmoregulatory metabolites were identified. All of these impacted on the photosynthetic rates of the different treatments. There were also differences in the growth of the fungi within the roots, and in the viability of the fungal structures. The comprehensive analysis of the plant–nematode–fungus group allows us to conclude that the isolate with the greatest capacity to compensate for the negative effect of parasitism, and with the greatest possibility of lasting as a biocontrol agent is Funneliformis mosseae.
{"title":"Comprehensive analysis of Nematode–Tomato Plant–Mycorrhizal Fungus system for bio based product development","authors":"Sebastián Andrés Garita, Valeria Fernanda Bernardo, Matías González, María Cecilia Arango, Marcela Ruscitti","doi":"10.1007/s11557-023-01939-9","DOIUrl":"https://doi.org/10.1007/s11557-023-01939-9","url":null,"abstract":"<p><i>Nacobbus aberrans</i> is a plant parasitic nematode that causes significant economic losses in the American Continent and is considered a quarantine pest in many countries. Some arbuscular mycorrhizal fungi have shown the ability to reduce the population of this pathogen; however, most studies do not consider other relevant aspects that have to do with the crop or the fungus. The purpose of this work was to select an isolated mycorrhizal fungus based on a comprehensive analysis of the Nematode–Plant–Mycorrhizal Fungus system. The test was conducted on <i>Solanum lycopersicum</i> L. grown in pots. The evaluated isolates were <i>Funneliformis mosseae</i>, <i>Rhizoglomus intraradices A2</i>, and <i>Rhizoglomus intraradices B1</i>. All the isolates reduced the nematode population significantly; however, differences in the content of photosynthetic pigments, soluble proteins, and osmoregulatory metabolites were identified. All of these impacted on the photosynthetic rates of the different treatments. There were also differences in the growth of the fungi within the roots, and in the viability of the fungal structures. The comprehensive analysis of the plant–nematode–fungus group allows us to conclude that the isolate with the greatest capacity to compensate for the negative effect of parasitism, and with the greatest possibility of lasting as a biocontrol agent is <i>Funneliformis mosseae</i>.</p>","PeriodicalId":19111,"journal":{"name":"Mycological Progress","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138818479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-12DOI: 10.1007/s11557-023-01937-x
Mateus Oliveira da Cruz, Hyang Burm Lee, Thalline Rafhaella Leite Cordeiro, Francisca Robervânia Soares dos Santos, Maria Helena Alves, Thuong Thuong Thi Nguyen, Eleni Gentekaki, Vedprakash G. Hurdeal, André Luiz Cabral Monteiro de Azevedo Santiago
During the assessment of coprophilous mucoralean diversity in northeastern areas of Brazil, a Lichtheimia-like specimen (URM 8358) was isolated from rabbit dung. It was characterized based on morphological, physiological, and molecular analyses of the internal transcribed spacer (ITS), large subunit ribosomal (LSU) and actin DNA sequences. These analyses revealed a novel Lichtheimia species, which is described herein. The novel species forms abundantly branched sporangiophores, with most branches arising near the main sporangia, various-shaped columellae, and irregularly shaped sporangiospores. Furthermore, this manuscript provides notes of all Lichtheimia species, and an identification key for the genus is provided. These findings contribute to the existing knowledge on the taxonomy and distribution of Lichtheimia. Because of the clinical relevance of other Lichtheimia species, we encourage future research to determine whether URM 8358 can be pathogenic to humans.
{"title":"Description of a novel coprophilous Lichtheimia (Mucoromycotina, Mucorales) species with notes on Lichtheimia species and an identification key for the genus","authors":"Mateus Oliveira da Cruz, Hyang Burm Lee, Thalline Rafhaella Leite Cordeiro, Francisca Robervânia Soares dos Santos, Maria Helena Alves, Thuong Thuong Thi Nguyen, Eleni Gentekaki, Vedprakash G. Hurdeal, André Luiz Cabral Monteiro de Azevedo Santiago","doi":"10.1007/s11557-023-01937-x","DOIUrl":"https://doi.org/10.1007/s11557-023-01937-x","url":null,"abstract":"<p>During the assessment of coprophilous mucoralean diversity in northeastern areas of Brazil, a <i>Lichtheimia</i>-like specimen (URM 8358) was isolated from rabbit dung. It was characterized based on morphological, physiological, and molecular analyses of the internal transcribed spacer (ITS), large subunit ribosomal (LSU) and actin DNA sequences. These analyses revealed a novel <i>Lichtheimia</i> species, which is described herein. The novel species forms abundantly branched sporangiophores, with most branches arising near the main sporangia, various-shaped columellae, and irregularly shaped sporangiospores. Furthermore, this manuscript provides notes of all <i>Lichtheimia</i> species, and an identification key for the genus is provided. These findings contribute to the existing knowledge on the taxonomy and distribution of <i>Lichtheimia</i>. Because of the clinical relevance of other <i>Lichtheimia</i> species, we encourage future research to determine whether URM 8358 can be pathogenic to humans.</p>","PeriodicalId":19111,"journal":{"name":"Mycological Progress","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138581110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-05DOI: 10.1007/s11557-023-01936-y
G. H. Jerônimo, D. R. Simmons, J. E. Longcore, T. Y. James, K. R. Amses, K. Seto, C. L. A. Pires-Zottarelli
Cladochytriales comprises a taxonomic order and lineage of chytrid fungi that are primarily saprobes of organic matter in aquatic and terrestrial ecosystems. The order contains species from 12 genera, though molecular confirmations of almost all type species are lacking, and many genera are considered polyphyletic. We conducted phylogenetic analyses of ribosomal RNA genes and developmental morphology of Cladochytriales isolates from the Collection of Zoosporic Eufungi at the University of Michigan and noted some strains that were distinct from described genera. In our phylogenetic analysis, Endochytriaceae represented by only one strain of Endochytrium ramosum was sister to Septochytriaceae while the incertae sedis lineage formed by the new species Thomasia carolinae was sister to Nowakowskiellaceae. Additionally, the phylogenetic relatedness of the other two new species, Dogmamyces elongatus and Allochytrium aureum, was also highly supported. A thorough revision of the order is needed because some genera, such as Catenochytridium and Nephrochytrium, remain polyphyletic or paraphyletic. In addition to indicating these problems, our updated phylogeny supports the description of two new genera and three new species and thereby begins to bring the knowledge of the Cladochytriales up to date.
{"title":"Investigation of CZEUM cultures reveals new genera and species of Cladochytriales (Cladochytriomycetes, Chytridiomycota)","authors":"G. H. Jerônimo, D. R. Simmons, J. E. Longcore, T. Y. James, K. R. Amses, K. Seto, C. L. A. Pires-Zottarelli","doi":"10.1007/s11557-023-01936-y","DOIUrl":"https://doi.org/10.1007/s11557-023-01936-y","url":null,"abstract":"<p><i>Cladochytriales</i> comprises a taxonomic order and lineage of chytrid fungi that are primarily saprobes of organic matter in aquatic and terrestrial ecosystems. The order contains species from 12 genera, though molecular confirmations of almost all type species are lacking, and many genera are considered polyphyletic. We conducted phylogenetic analyses of ribosomal RNA genes and developmental morphology of <i>Cladochytriales</i> isolates from the Collection of Zoosporic Eufungi at the University of Michigan and noted some strains that were distinct from described genera. In our phylogenetic analysis, <i>Endochytriaceae</i> represented by only one strain of <i>Endochytrium ramosum</i> was sister to <i>Septochytriaceae</i> while the <i>incertae sedis</i> lineage formed by the new species <i>Thomasia carolinae</i> was sister to <i>Nowakowskiellaceae</i>. Additionally, the phylogenetic relatedness of the other two new species, <i>Dogmamyces elongatus</i> and <i>Allochytrium aureum</i>, was also highly supported. A thorough revision of the order is needed because some genera, such as <i>Catenochytridium</i> and <i>Nephrochytrium</i>, remain polyphyletic or paraphyletic. In addition to indicating these problems, our updated phylogeny supports the description of two new genera and three new species and thereby begins to bring the knowledge of the <i>Cladochytriales</i> up to date.</p>","PeriodicalId":19111,"journal":{"name":"Mycological Progress","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138509875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-02DOI: 10.1007/s11557-023-01933-1
Amanda Barreto Xavier-Leite, Bruno Tomio Goto, Robert Lücking, Marcela Eugenia da Silva Cáceres
Previous molecular phylogenetic analysis in comparison with phenotype characters of Gomphillaceae resolved a total of 38 genus-level clades among the studied species, 17 of which in need to be recognized and formally established as new or reinstated genera. The four reinstated genus names, including one validation, are Microxyphiomyces Bat., Valle & Peres, Psathyromyces Bat. & Peres, Spinomyces Bat. & Peres ex Xavier-Leite, M. Cáceres & Lücking, gen. nov., and Sporocybomyces H. Maia. The remaining 13 are newly described: Adelphomyces Xavier-Leite, M. Cáceres & Lücking, gen. nov., Aptrootidea Xavier-Leite, M. Cáceres & Lücking, gen. nov., Aulaxinella Xavier-Leite, M. Cáceres & Lücking, gen. nov., Batistomyces Xavier-Leite, M. Cáceres & Lücking, gen. nov., Bezerroplaca Xavier-Leite, M. Cáceres & Lücking, gen. nov., Caleniella Xavier-Leite, M. Cáceres & Lücking, gen. nov., Monocalenia Xavier-Leite, M. Cáceres & Lücking, gen. nov., Pseudocalenia Xavier-Leite, M. Cáceres & Lücking, gen. nov., Roselviria Xavier-Leite, M. Cáceres & Lücking, gen. nov., Santricharia Xavier-Leite, M. Cáceres & Lücking, gen. nov., Sipmanidea Xavier-Leite, M. Cáceres & Lücking, gen. nov., Verruciplaca Xavier-Leite, M. Cáceres & Lücking, gen. nov., and Vezdamyces Xavier-Leite, M. Cáceres & Lücking, gen. nov. The following 53 new combinations are introduced for the species included in the new and reinstated genera: Adelphomyces cochlearifer (Lücking & Sérus.) Xavier-Leite, M. Cáceres & Lücking, comb. nov., A. epithallina (Lücking) Xavier-Leite, M. Cáceres & Lücking, comb. nov., A. parvula (Hafellner & Vězda) Xavier-Leite, M. Cáceres & Lücking, comb. nov., Aptrootidea amapensis (Bat. & Poroca) Xavier-Leite, M. Cáceres & Lücking, comb. nov., A. atrofusca (R. Sant.) Xavier-Leite, M. Cáceres & Lücking, comb. nov., A. atromuralis (Lücking) Xavier-Leite, M. Cáceres & Lücking, comb. nov., A. marginata (Lücking) Xavier-Leite, M. Cáceres & Lücking, comb. nov., A. triseptata (Lücking) Xavier-Leite, M. Cáceres & Lücking, comb. nov., A. wilsoniorum (Lücking) Xavier-Leite, M. Cáceres & Lücking, comb. nov., Aulaxinella corticola (Kalb & Vězda) Xavier-Leite, M. Cáceres & Lücking, comb. nov., A. minuta (R. Sant.) Xavier-Leite, M. Cáceres & Lücking, comb. nov., A. multiseptata (R. Sant.) Xavier-Leite, M. Cáceres & Lücking, comb. nov., Batistomyces pallidus (Vězda) Xavier-Leite, M. Cáceres & Lücking, comb. nov., B. hyalinus (Kalb & Vězda) Xavier-Leite, M. Cáceres & Lücking, comb. nov., Bezerroplaca fusconitida (Lücking) Xavier-Leite, M. Cáceres & Lücking, comb. nov., B. incrustatociliata (Sérus.) Xavier-Leite, M.
{"title":"New genera in the lichenized family Gomphillaceae (Ascomycota: Graphidales) focusing on neotropical taxa","authors":"Amanda Barreto Xavier-Leite, Bruno Tomio Goto, Robert Lücking, Marcela Eugenia da Silva Cáceres","doi":"10.1007/s11557-023-01933-1","DOIUrl":"https://doi.org/10.1007/s11557-023-01933-1","url":null,"abstract":"<p>Previous molecular phylogenetic analysis in comparison with phenotype characters of <i>Gomphillaceae</i> resolved a total of 38 genus-level clades among the studied species, 17 of which in need to be recognized and formally established as new or reinstated genera. The four reinstated genus names, including one validation, are <i>Microxyphiomyces</i> Bat., Valle & Peres, <i>Psathyromyces</i> Bat. & Peres, <i>Spinomyces</i> Bat. & Peres ex Xavier-Leite, M. Cáceres & Lücking, gen. nov., and <i>Sporocybomyces</i> H. Maia. The remaining 13 are newly described: <i>Adelphomyces</i> Xavier-Leite, M. Cáceres & Lücking, gen. nov., <i>Aptrootidea</i> Xavier-Leite, M. Cáceres & Lücking, gen. nov., <i>Aulaxinella</i> Xavier-Leite, M. Cáceres & Lücking, gen. nov., <i>Batistomyces</i> Xavier-Leite, M. Cáceres & Lücking, gen. nov., <i>Bezerroplaca</i> Xavier-Leite, M. Cáceres & Lücking, gen. nov., <i>Caleniella</i> Xavier-Leite, M. Cáceres & Lücking, gen. nov., <i>Monocalenia</i> Xavier-Leite, M. Cáceres & Lücking, gen. nov., <i>Pseudocalenia</i> Xavier-Leite, M. Cáceres & Lücking, gen. nov., <i>Roselviria</i> Xavier-Leite, M. Cáceres & Lücking, gen. nov., <i>Santricharia</i> Xavier-Leite, M. Cáceres & Lücking, gen. nov., <i>Sipmanidea</i> Xavier-Leite, M. Cáceres & Lücking, gen. nov., <i>Verruciplaca</i> Xavier-Leite, M. Cáceres & Lücking, gen. nov., and <i>Vezdamyces</i> Xavier-Leite, M. Cáceres & Lücking, gen. nov. The following 53 new combinations are introduced for the species included in the new and reinstated genera: <i>Adelphomyces cochlearifer</i> (Lücking & Sérus.) Xavier-Leite, M. Cáceres & Lücking, comb. nov., <i>A. epithallina</i> (Lücking) Xavier-Leite, M. Cáceres & Lücking, comb. nov., <i>A. parvula</i> (Hafellner & Vězda) Xavier-Leite, M. Cáceres & Lücking, comb. nov., <i>Aptrootidea amapensis</i> (Bat. & Poroca) Xavier-Leite, M. Cáceres & Lücking, comb. nov., <i>A. atrofusca</i> (R. Sant.) Xavier-Leite, M. Cáceres & Lücking, comb. nov., <i>A. atromuralis</i> (Lücking) Xavier-Leite, M. Cáceres & Lücking, comb. nov., <i>A. marginata</i> (Lücking) Xavier-Leite, M. Cáceres & Lücking, comb. nov., <i>A. triseptata</i> (Lücking) Xavier-Leite, M. Cáceres & Lücking, comb. nov., <i>A. wilsoniorum</i> (Lücking) Xavier-Leite, M. Cáceres & Lücking, comb. nov., <i>Aulaxinella corticola</i> (Kalb & Vězda) Xavier-Leite, M. Cáceres & Lücking, comb. nov., <i>A. minuta</i> (R. Sant.) Xavier-Leite, M. Cáceres & Lücking, comb. nov., <i>A. multiseptata</i> (R. Sant.) Xavier-Leite, M. Cáceres & Lücking, comb. nov., <i>Batistomyces pallidus</i> (Vězda) Xavier-Leite, M. Cáceres & Lücking, comb. nov., <i>B. hyalinus</i> (Kalb & Vězda) Xavier-Leite, M. Cáceres & Lücking, comb. nov., <i>Bezerroplaca fusconitida</i> (Lücking) Xavier-Leite, M. Cáceres & Lücking, comb. nov., <i>B. incrustatociliata</i> (Sérus.) Xavier-Leite, M.","PeriodicalId":19111,"journal":{"name":"Mycological Progress","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138509864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-02DOI: 10.1007/s11557-023-01935-z
Akira Hashimoto, Saho Shibata, Moriya Ohkuma
Antherospora vaillantii is an anther smut fungus that was originally considered occurring on Muscari spp. around the world; however, cryptic species were revealed during recent taxonomic investigations, thus emphasizing the importance of host specificity. In Japan, A. vaillantii was identified on Barnardia japonica in 1936. Although the recent revelations indicate that it may be part of a complex based on the host plant genus, the phylogenetic placement of this Japanese species is currently unresolved. In this investigation, seven specimens of the A. vaillantii complex were collected from the Kanto area of Japan, and six new single teliospore isolates were established. Molecular phylogenetic analyses based on the internal transcribed spacer sequences and large subunit nuclear ribosomal DNA (28S) region indicated that the new samples formed a monophyletic clade with other Antherospora spp. among Floromycetaceae lineage. Our samples were separated from other known species of Antherospora and formed a robust monophyletic clade in any analysis. Detailed morphological observations revealed that the new samples could be distinguished from other known Antherospora species. As a result, it is proposed that Antherospora barnardiae can accommodate B. japonica parasites.
{"title":"Phylogenetic and morphological re-evaluation of Antherospora on Barnardia japonica collected in Japan","authors":"Akira Hashimoto, Saho Shibata, Moriya Ohkuma","doi":"10.1007/s11557-023-01935-z","DOIUrl":"https://doi.org/10.1007/s11557-023-01935-z","url":null,"abstract":"<p>\u0000<i>Antherospora vaillantii</i> is an anther smut fungus that was originally considered occurring on <i>Muscari</i> spp. around the world; however, cryptic species were revealed during recent taxonomic investigations, thus emphasizing the importance of host specificity. In Japan, <i>A. vaillantii</i> was identified on <i>Barnardia japonica</i> in 1936. Although the recent revelations indicate that it may be part of a complex based on the host plant genus, the phylogenetic placement of this Japanese species is currently unresolved. In this investigation, seven specimens of the <i>A. vaillantii</i> complex were collected from the Kanto area of Japan, and six new single teliospore isolates were established. Molecular phylogenetic analyses based on the internal transcribed spacer sequences and large subunit nuclear ribosomal DNA (28S) region indicated that the new samples formed a monophyletic clade with other <i>Antherospora</i> spp. among <i>Floromycetaceae</i> lineage. Our samples were separated from other known species of <i>Antherospora</i> and formed a robust monophyletic clade in any analysis. Detailed morphological observations revealed that the new samples could be distinguished from other known <i>Antherospora</i> species. As a result, it is proposed that <i>Antherospora barnardiae</i> can accommodate <i>B. japonica</i> parasites.</p>","PeriodicalId":19111,"journal":{"name":"Mycological Progress","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138509877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-30DOI: 10.1007/s11557-023-01938-w
Anthony T. Buaya, Ichen Tsai, Tatyana A. Klochkova, Marco Thines
Holocarpic oomycetes, which had fallen into relative scientific obscurity for quite some time, recently regained scientific interest. Among these organisms, some stand out for infecting commercially valuable red algae, such as the nori algae of the genus Pyropia, rendering them economically significant. After having observed simple holocarpic pathogens of green algae in previous sampling trips, most of which we could not fully characterise due to their scarcity, we now discovered a parasite of the widespread littoral green algae Urospora neglecta. This previously unnoticed parasite turned out to be distinct from another green algae-infesting species, Sirolpidium bryopsidis. This finding led us to formally describe it as Sirolpidium litorale, marking the first new species of holocarpic oomycetes of green algae to be found on the shores of Blávík, East Iceland. The ease of cultivation of U. neglecta offers a unique opportunity to establish a genetically tractable system for studying how Sirolpidium interacts with algae. This could help uncover the underlying mechanisms behind the pathogenicity of the species of this genus and provide valuable insights into their ecological roles and evolutionary history.
{"title":"Introducing a new pathosystem for marine pathogens – the green alga Urospora neglecta and its pathogen Sirolpidium litorale sp. nov.","authors":"Anthony T. Buaya, Ichen Tsai, Tatyana A. Klochkova, Marco Thines","doi":"10.1007/s11557-023-01938-w","DOIUrl":"https://doi.org/10.1007/s11557-023-01938-w","url":null,"abstract":"<p>Holocarpic oomycetes, which had fallen into relative scientific obscurity for quite some time, recently regained scientific interest. Among these organisms, some stand out for infecting commercially valuable red algae, such as the nori algae of the genus <i>Pyropia</i>, rendering them economically significant. After having observed simple holocarpic pathogens of green algae in previous sampling trips, most of which we could not fully characterise due to their scarcity, we now discovered a parasite of the widespread littoral green algae <i>Urospora neglecta</i>. This previously unnoticed parasite turned out to be distinct from another green algae-infesting species, <i>Sirolpidium bryopsidis</i>. This finding led us to formally describe it as <i>Sirolpidium litorale</i>, marking the first new species of holocarpic oomycetes of green algae to be found on the shores of Blávík, East Iceland. The ease of cultivation of <i>U. neglecta</i> offers a unique opportunity to establish a genetically tractable system for studying how <i>Sirolpidium</i> interacts with algae. This could help uncover the underlying mechanisms behind the pathogenicity of the species of this genus and provide valuable insights into their ecological roles and evolutionary history.</p>","PeriodicalId":19111,"journal":{"name":"Mycological Progress","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138529462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Three new Clavulina species, namely C. baiyunensis, C. minor, and C. lilaceorosea, were described from specimens collected from Baiyun Mountain, Guangzhou, China, based on detailed morphological studies and phylogenetic analyses of the nucleotide sequences of three loci (ITS, LSU, and RPB2). Clavulina baiyunensis is characterized by light brown to brown basidiomata with hyaline tramal hyphae. Clavulina minor has diminutive, dull white to pale brown basidiomata with hyaline tramal hyphae. Clavulina lilaceorosea has pale brown to ochre brown basidiomata with palmate branches, and light brown walls of tramal hyphae. All three new species have abundant clamp connections. A key to the known species of Clavulina from China is provided.
{"title":"Three new Clavulina species from Baiyun Mountain, Guangzhou, China","authors":"Xiao-Xia Huang, Yi-Xin Chen, Zhi-Jun Lin, Ying-Shan Liang, Li-Hong Qiu","doi":"10.1007/s11557-023-01929-x","DOIUrl":"https://doi.org/10.1007/s11557-023-01929-x","url":null,"abstract":"<p>Three new <i>Clavulina</i> species, namely <i>C. baiyunensis</i>, <i>C. minor</i>, and <i>C. lilaceorosea</i>, were described from specimens collected from Baiyun Mountain, Guangzhou, China, based on detailed morphological studies and phylogenetic analyses of the nucleotide sequences of three loci (ITS, LSU, and <i>RPB2</i>). <i>Clavulina baiyunensis</i> is characterized by light brown to brown basidiomata with hyaline tramal hyphae. <i>Clavulina minor</i> has diminutive, dull white to pale brown basidiomata with hyaline tramal hyphae. <i>Clavulina lilaceorosea</i> has pale brown to ochre brown basidiomata with palmate branches, and light brown walls of tramal hyphae. All three new species have abundant clamp connections. A key to the known species of <i>Clavulina</i> from China is provided.</p><h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>\u0000","PeriodicalId":19111,"journal":{"name":"Mycological Progress","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138529472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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