I. O. Ogbonna, J. Ikwebe, J. Ogbonna, C. Eze, J.B. Ndrimbula
Microalgae oil accumulation in their natural growing states hardly reaches the quantities needed to replace fossil-derived diesel. Therefore, for algae to be used as biofuel feedstock, their growths are manipulated with the aim of achieving high cell density and high lipid accumulation. Two major factors affecting microalgal biomass and lipid productivity are light intensity and photoperiod. In the present study, Desmodesmus subspicatus was grown photoautotrophically under varying light intensities and photoperiods with a view to assessing the growth, lipid accumulation potential and fatty acid composition. Whereas the optimal light intensity for biomass production of the microalga was 5000 lx, that for lipid productivity was 3500 lx. At 5000 lx and 18: 6 h light/dark cycle, biomass yield, lipid content and lipid productivity were highest, at values of 1.92 ± 0.03 g/L, 53% and 118.80 ± 2.04 mgL-1day -1 respectively. The major fatty acid of the alga was oleic acid irrespective of light changes. The quantities of lipid accumulated and the properties of the fatty acid methyl esters showed that Desmodesmus subspicatus LC172266 is an ideal feedstock for biodiesel production.
{"title":"Effects of light intensity and photoperiod on growth, lipid accumulation and fatty acid composition of Desmodesmus subspicatus LC172266 under photoautotrophic cultivation","authors":"I. O. Ogbonna, J. Ikwebe, J. Ogbonna, C. Eze, J.B. Ndrimbula","doi":"10.4314/njb.v38i2.1","DOIUrl":"https://doi.org/10.4314/njb.v38i2.1","url":null,"abstract":"Microalgae oil accumulation in their natural growing states hardly reaches the quantities needed to replace fossil-derived diesel. Therefore, for algae to be used as biofuel feedstock, their growths are manipulated with the aim of achieving high cell density and high lipid accumulation. Two major factors affecting microalgal biomass and lipid productivity are light intensity and photoperiod. In the present study, Desmodesmus subspicatus was grown photoautotrophically under varying light intensities and photoperiods with a view to assessing the growth, lipid accumulation potential and fatty acid composition. Whereas the optimal light intensity for biomass production of the microalga was 5000 lx, that for lipid productivity was 3500 lx. At 5000 lx and 18: 6 h light/dark cycle, biomass yield, lipid content and lipid productivity were highest, at values of 1.92 ± 0.03 g/L, 53% and 118.80 ± 2.04 mgL-1day -1 respectively. The major fatty acid of the alga was oleic acid irrespective of light changes. The quantities of lipid accumulated and the properties of the fatty acid methyl esters showed that Desmodesmus subspicatus LC172266 is an ideal feedstock for biodiesel production.","PeriodicalId":19168,"journal":{"name":"Nigerian Journal of Biotechnology","volume":"61 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86054604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F.A. Fashola, O. Fadipe, P. N. Nwagala, S. O. Olatope, C.P. Augustine, O. I. Ibidapo, I. James, F.B. Aderinwale, F. Orji, A. Lawal
Microbial alkaline protease is one of the dominant industrial enzymes which function in splitting polypeptides chain of protein into monomers of amino acids and peptides. This study aimed to identify alkaline protease produced by Bacillus sp. Soil samples were aseptically collected from dump sites in FIIRO, Lagos state, Nigeria. The samples were serially diluted, and bacteria were isolated using pour plate method. The resulting isolates were screened and morphologically characterized. The isolate with the highest protease production potential was subjected to biochemical characterization using Analytical Profile Index (API) identification kit system and 16S rRNA sequencing. The selected isolate was used to produce alkaline protease by solid state fermentation using rice bran as a substrate. Out of the 18 bacteria isolated, 11 isolates showed alkaline protease production potential. Isolate C3a-FIIRO was selected for its maximal alkaline protease produced as indicated by a 56 mm zone of clearance. Morphological and biochemical characterization revealed isolate C3a-FIIRO as a member of the genus Bacillus. The 16S rRNA gene sequencing confirmed the isolate as Bacillus subtilis C3a-FIIRO (MW577298) with closest homology to Bacillus subtilis Y17B. The enzyme activity of 6848.171 U/ml ± 0.11 and protein concentration of 152.13 mg/ml ± 0.003 showed that Bacillus subtilis C3a-FIIRO has potential for sustainable alkaline protease production.
{"title":"Characterization of Novel Alkaline Protease producing Bacillus subtilis C3a-FIIRO with Potential for Industrial Application","authors":"F.A. Fashola, O. Fadipe, P. N. Nwagala, S. O. Olatope, C.P. Augustine, O. I. Ibidapo, I. James, F.B. Aderinwale, F. Orji, A. Lawal","doi":"10.4314/njb.v38i2.6","DOIUrl":"https://doi.org/10.4314/njb.v38i2.6","url":null,"abstract":"Microbial alkaline protease is one of the dominant industrial enzymes which function in splitting polypeptides chain of protein into monomers of amino acids and peptides. This study aimed to identify alkaline protease produced by Bacillus sp. Soil samples were aseptically collected from dump sites in FIIRO, Lagos state, Nigeria. The samples were serially diluted, and bacteria were isolated using pour plate method. The resulting isolates were screened and morphologically characterized. The isolate with the highest protease production potential was subjected to biochemical characterization using Analytical Profile Index (API) identification kit system and 16S rRNA sequencing. The selected isolate was used to produce alkaline protease by solid state fermentation using rice bran as a substrate. Out of the 18 bacteria isolated, 11 isolates showed alkaline protease production potential. Isolate C3a-FIIRO was selected for its maximal alkaline protease produced as indicated by a 56 mm zone of clearance. Morphological and biochemical characterization revealed isolate C3a-FIIRO as a member of the genus Bacillus. The 16S rRNA gene sequencing confirmed the isolate as Bacillus subtilis C3a-FIIRO (MW577298) with closest homology to Bacillus subtilis Y17B. The enzyme activity of 6848.171 U/ml ± 0.11 and protein concentration of 152.13 mg/ml ± 0.003 showed that Bacillus subtilis C3a-FIIRO has potential for sustainable alkaline protease production.","PeriodicalId":19168,"journal":{"name":"Nigerian Journal of Biotechnology","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73700530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
U. Onwuzuruike, C. J. Okakpu, J. Ndife, U. Uzochukwu, O. Ubochi
Three different methods, including Soxhlet, traditional and screw press, were used to extract oil from African pear pulp. The oils were analysed for selected minerals and phytonutrients; stored for 4 weeks and analysed for oxidative changes. The micro-components results ranged from 0.00 to 41.50% for chlorophyll, 0.00 to 1.25 µg/100g for total carotenoids, 0.02 to 0.04 mg/100g for iron, 0.00 to 0.01 mg/100g for copper, and 18.25 to 208.50 mg/100g for vitamin E. The oxidative stability results showed that peroxide values ranged from 0.13 to 2.69 meq O2/kg in week 1 to 0.92 to 3.62 meq O2/kg in week 4; FFA ranged from 0.36 to 7.04% in week 1 to 0.39 to 11.47% in week 4; density ranged from 0.89 to 0.91 g/cm3 in week 1 to 0.91 to 0.98 g/cm3 in week 4; viscosity ranged from 17.17 to 51.57 RVU in week 1 to 20.31 to 55.05 RVU in week 4 and thiobarbituric acid level ranged from 0.02 to 1.11 mg/g in week 1 to 0.09 to 1.31 mg/g in week 4. Soxhlet extraction method produced oil of best quality with significantly (p < 0.05) higher mineral and phytonutrient contents, and of a longer storage stability.
{"title":"Effect of different extraction methods on micro-component composition and oxidative stability of oil produced from African pear (Dacryodes edulis) mesocarp Oil","authors":"U. Onwuzuruike, C. J. Okakpu, J. Ndife, U. Uzochukwu, O. Ubochi","doi":"10.4314/njb.v38i2.2","DOIUrl":"https://doi.org/10.4314/njb.v38i2.2","url":null,"abstract":"Three different methods, including Soxhlet, traditional and screw press, were used to extract oil from African pear pulp. The oils were analysed for selected minerals and phytonutrients; stored for 4 weeks and analysed for oxidative changes. The micro-components results ranged from 0.00 to 41.50% for chlorophyll, 0.00 to 1.25 µg/100g for total carotenoids, 0.02 to 0.04 mg/100g for iron, 0.00 to 0.01 mg/100g for copper, and 18.25 to 208.50 mg/100g for vitamin E. The oxidative stability results showed that peroxide values ranged from 0.13 to 2.69 meq O2/kg in week 1 to 0.92 to 3.62 meq O2/kg in week 4; FFA ranged from 0.36 to 7.04% in week 1 to 0.39 to 11.47% in week 4; density ranged from 0.89 to 0.91 g/cm3 in week 1 to 0.91 to 0.98 g/cm3 in week 4; viscosity ranged from 17.17 to 51.57 RVU in week 1 to 20.31 to 55.05 RVU in week 4 and thiobarbituric acid level ranged from 0.02 to 1.11 mg/g in week 1 to 0.09 to 1.31 mg/g in week 4. Soxhlet extraction method produced oil of best quality with significantly (p < 0.05) higher mineral and phytonutrient contents, and of a longer storage stability.","PeriodicalId":19168,"journal":{"name":"Nigerian Journal of Biotechnology","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82222553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Muhammad, F. Yusuf, F. A. Ahmad, U. Shehu, H. Yakasai
The widespread and long term use of atrazine results in high residue levels of atrazine in soil, which further causes water contamination, it is considered as an endocrine disruptor and is potentially carcinogenic. Microbial degradation of herbicide represents a time cost effective way of eco-restoration. This research was aimed at isolating and characterizing bacteria capable of degrading and utilizing atrazine as a sole carbon source. An enrichment method was used to isolate the bacteria on mineral salt media (MSM) following serial dilution. The isolate was identified morphologically, biochemically and molecularly as Bacillus safensis strain BUK_BCH_BTE6 based on 16S rRNA gene sequence and molecular phylogenetic analysis. The effect of pH and temperature on the degradation of atrazine was studied in MSM medium supplemented with atrazine as sole carbon source. Growth and degradation of atrazine in this isolate was optimal at pH 7.5 and temperature of 35 °C. Bacillus safensis is highly efficient in atrazine degradation with an optimum range of pH and temperature. Bacillus safensis could be a suitable candidate for bioremediation of atrazine polluted sites.
{"title":"Optimizing the Effect of pH and Temperature on Atrazine Degradation by Bacillus safensis strain BUK_BCH_BTE6 an Efficient Atrazine Tolerating Bacteria from an Agricultural Soil in Kura Local Government Area of Kano State, Nigeria","authors":"F. Muhammad, F. Yusuf, F. A. Ahmad, U. Shehu, H. Yakasai","doi":"10.4314/njb.v38i2.10","DOIUrl":"https://doi.org/10.4314/njb.v38i2.10","url":null,"abstract":"The widespread and long term use of atrazine results in high residue levels of atrazine in soil, which further causes water contamination, it is considered as an endocrine disruptor and is potentially carcinogenic. Microbial degradation of herbicide represents a time cost effective way of eco-restoration. This research was aimed at isolating and characterizing bacteria capable of degrading and utilizing atrazine as a sole carbon source. An enrichment method was used to isolate the bacteria on mineral salt media (MSM) following serial dilution. The isolate was identified morphologically, biochemically and molecularly as Bacillus safensis strain BUK_BCH_BTE6 based on 16S rRNA gene sequence and molecular phylogenetic analysis. The effect of pH and temperature on the degradation of atrazine was studied in MSM medium supplemented with atrazine as sole carbon source. Growth and degradation of atrazine in this isolate was optimal at pH 7.5 and temperature of 35 °C. Bacillus safensis is highly efficient in atrazine degradation with an optimum range of pH and temperature. Bacillus safensis could be a suitable candidate for bioremediation of atrazine polluted sites.","PeriodicalId":19168,"journal":{"name":"Nigerian Journal of Biotechnology","volume":"216 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85528059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
U. Onwuzuruike, K. G. Okakpu, J. Ndife, U. Uzochukwu, E.U. Abiamere
This study evaluated the quality characteristics of strips produced from blends (%) of soybean and cassava flours. Wholesome cassava roots and soybean grains were procured and processed into flours. Seven blends of soybean and cassava flour blends were formulated (0:100, 100:0, 50:50, 40:60, 20:80, 60:40 and 80:20) while 100% wheat flour served as control. Strips were produced from the blends. The composite flours were analyzed for their functional properties while the proximate, mineral, vitamin, antinutrient contents and sensory characteristics (assessed by 25 panelists) of the strips were assessed. The statistical analysis was carried out using one-way ANOVA at 95% confidence level. The functional properties ranged from 0.53 to 0.77 g/mL for bulk density, 3.76 to 12.88% for foam capacity, 1.92 to 49.00% for foam stability, 108.00 to 204.00 g/mL for oil absorption capacity, 81.00 to 140.50 g/mL for water absorption capacity and 61.07 to 87.00°C for gelatinization temperature. The results of proximate analysis ranged from 1.44 to 3.46% for moisture, 1.63 to 3.91% for ash, 2.04 to 10.76% for fat, 0.18 to 1.37% for crude fibre, 4.07 to 24.16% for protein and 60.46 to 88.10% for carbohydrate. The phytate, tannin, trypsin inhibitor, saponin and hydrogen cyanide ranged from 0.22 to 5.59 mg/100g, 0.34 to 4.13 mg/100g, 0.04 to 1.69 mg/100g, 0.02 to 0.36 mg/100g and 0.00 to 2.11 mg/100g respectively. Sensory analysis revealed that 80% soybean flour and 20% cassava flour possessed better organoleptic characteristics. Production of strips from blends of soybean and cassava flours improved the nutrient composition, sensory properties with acceptable/safe antinutrient limit which can be optimized for value addition.
{"title":"Quality Assessment of Strips produced from Soybean and Cassava Flour Blends","authors":"U. Onwuzuruike, K. G. Okakpu, J. Ndife, U. Uzochukwu, E.U. Abiamere","doi":"10.4314/njb.v38i2.3","DOIUrl":"https://doi.org/10.4314/njb.v38i2.3","url":null,"abstract":"This study evaluated the quality characteristics of strips produced from blends (%) of soybean and cassava flours. Wholesome cassava roots and soybean grains were procured and processed into flours. Seven blends of soybean and cassava flour blends were formulated (0:100, 100:0, 50:50, 40:60, 20:80, 60:40 and 80:20) while 100% wheat flour served as control. Strips were produced from the blends. The composite flours were analyzed for their functional properties while the proximate, mineral, vitamin, antinutrient contents and sensory characteristics (assessed by 25 panelists) of the strips were assessed. The statistical analysis was carried out using one-way ANOVA at 95% confidence level. The functional properties ranged from 0.53 to 0.77 g/mL for bulk density, 3.76 to 12.88% for foam capacity, 1.92 to 49.00% for foam stability, 108.00 to 204.00 g/mL for oil absorption capacity, 81.00 to 140.50 g/mL for water absorption capacity and 61.07 to 87.00°C for gelatinization temperature. The results of proximate analysis ranged from 1.44 to 3.46% for moisture, 1.63 to 3.91% for ash, 2.04 to 10.76% for fat, 0.18 to 1.37% for crude fibre, 4.07 to 24.16% for protein and 60.46 to 88.10% for carbohydrate. The phytate, tannin, trypsin inhibitor, saponin and hydrogen cyanide ranged from 0.22 to 5.59 mg/100g, 0.34 to 4.13 mg/100g, 0.04 to 1.69 mg/100g, 0.02 to 0.36 mg/100g and 0.00 to 2.11 mg/100g respectively. Sensory analysis revealed that 80% soybean flour and 20% cassava flour possessed better organoleptic characteristics. Production of strips from blends of soybean and cassava flours improved the nutrient composition, sensory properties with acceptable/safe antinutrient limit which can be optimized for value addition.","PeriodicalId":19168,"journal":{"name":"Nigerian Journal of Biotechnology","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81904694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nanoparticles are substances ranging from 1 – 100 nm in size and they have improved property such as increased surface area to volume ratio. In this study, in vitro antifungal activities of mancozeb/phytosynthesized zinc oxide nanoparticles against Eurotium sp. isolated from diseased cassava plants (Manihot esculenta crantz) were determined. Zinc oxide (ZnO) nanoparticles was synthesized using Moringa leaf extract and characterization of the biosynthesized nanoparticles was done using UV-Vis spectrophotometer. Different combinationsof zinc oxide nanoparticles and mancozeb were prepared corresponding to 25/75, 50/50 and 75/25 ZnO nanoparticles/mancozeb respectively. Antifungal testing using the test nanoparticles/mancozeb combinations was carried out using thefood poisoning method. The results obtained from this study indicate that zinc oxide nanoparticles/mancozeb combinations significantly inhibited the growth of the test pathogen with varying rates of inhibition. One hundred percent (100 %) inhibition of Eurotium sp. was obtained by 25/75, 75/25 and 100 % nanoparticles treatments. Future direction to this study is to investigate how ZnO nanoparticles/mancozeb combinations could be used for crop protection against phytopathogens. The mode of action of the test nanoparticles should be further investigated.
{"title":"In vitro antifungal activities of mancozeb/phytosynthesized zinc oxide nanoparticles against Eurotium sp. isolated from diseased cassava plant (Manihot esculenta Crantz)","authors":"O. H. Obiazikwor, J.I. Ojeile","doi":"10.4314/njb.v38i2.5","DOIUrl":"https://doi.org/10.4314/njb.v38i2.5","url":null,"abstract":"Nanoparticles are substances ranging from 1 – 100 nm in size and they have improved property such as increased surface area to volume ratio. In this study, in vitro antifungal activities of mancozeb/phytosynthesized zinc oxide nanoparticles against Eurotium sp. isolated from diseased cassava plants (Manihot esculenta crantz) were determined. Zinc oxide (ZnO) nanoparticles was synthesized using Moringa leaf extract and characterization of the biosynthesized nanoparticles was done using UV-Vis spectrophotometer. Different combinationsof zinc oxide nanoparticles and mancozeb were prepared corresponding to 25/75, 50/50 and 75/25 ZnO nanoparticles/mancozeb respectively. Antifungal testing using the test nanoparticles/mancozeb combinations was carried out using thefood poisoning method. The results obtained from this study indicate that zinc oxide nanoparticles/mancozeb combinations significantly inhibited the growth of the test pathogen with varying rates of inhibition. One hundred percent (100 %) inhibition of Eurotium sp. was obtained by 25/75, 75/25 and 100 % nanoparticles treatments. Future direction to this study is to investigate how ZnO nanoparticles/mancozeb combinations could be used for crop protection against phytopathogens. The mode of action of the test nanoparticles should be further investigated.","PeriodicalId":19168,"journal":{"name":"Nigerian Journal of Biotechnology","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80869451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The growing number of antimicrobial resistant bacteria have necessitated urgent need for newer and effective antimicrobials. Antimicrobial peptides (AMPs) which are also called host defense peptides are promising class of bioactives found in all living thing especially amphibians. A total of 355 Sclerophrys regularis were collected and each toad was weighed and 0.02mL/g of epinephrine (1mg/mL) was administered via the dorsal lymph sac of the toad. The skin secretions were washed, lyophilized and stored at -20°C. The secretion was purified using Sephadex G-50 gel purification column. The fractions showing antimicrobial activities against Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, Salmonella typhii ATCC 14028, Citrobacter freundii ATCC 8090, Staphylococcus aureus ATCC 25913 and Candida albicans ATCC 3147 were further purified by RP- HPFC. The purified fractions were lyophilized and m/z ratio peaks were determined using MALDI TOF/TOF MS. The sequences corresponding to the peaks were determined using Mascot Peptide Mass Fingerprint which revealed novel AMP sequences: IHAGKTVPIVK, MHLLWR and LTGQIKNGLSGR which were named Regularin-2, Regularin-3 and Regularin-4 respectively. The MICs of purified fractions were between 50 – 200 µg/mL and the antimicrobial activities were maintained when stored at -20 °C for 180 days and was significant at p < 0.05. Bioprospecting of Nigerian toad specie (S. regularis) could yield potential therapeutic AMP agents.
{"title":"Characterization of Some Novel Antimicrobial Peptides from African Common Toad, Sclerophrys regularis","authors":"A. Aliyu, Y. Ibrahim, B. Tytler","doi":"10.4314/njb.v38i2.9","DOIUrl":"https://doi.org/10.4314/njb.v38i2.9","url":null,"abstract":"The growing number of antimicrobial resistant bacteria have necessitated urgent need for newer and effective antimicrobials. Antimicrobial peptides (AMPs) which are also called host defense peptides are promising class of bioactives found in all living thing especially amphibians. A total of 355 Sclerophrys regularis were collected and each toad was weighed and 0.02mL/g of epinephrine (1mg/mL) was administered via the dorsal lymph sac of the toad. The skin secretions were washed, lyophilized and stored at -20°C. The secretion was purified using Sephadex G-50 gel purification column. The fractions showing antimicrobial activities against Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, Salmonella typhii ATCC 14028, Citrobacter freundii ATCC 8090, Staphylococcus aureus ATCC 25913 and Candida albicans ATCC 3147 were further purified by RP- HPFC. The purified fractions were lyophilized and m/z ratio peaks were determined using MALDI TOF/TOF MS. The sequences corresponding to the peaks were determined using Mascot Peptide Mass Fingerprint which revealed novel AMP sequences: IHAGKTVPIVK, MHLLWR and LTGQIKNGLSGR which were named Regularin-2, Regularin-3 and Regularin-4 respectively. The MICs of purified fractions were between 50 – 200 µg/mL and the antimicrobial activities were maintained when stored at -20 °C for 180 days and was significant at p < 0.05. Bioprospecting of Nigerian toad specie (S. regularis) could yield potential therapeutic AMP agents.","PeriodicalId":19168,"journal":{"name":"Nigerian Journal of Biotechnology","volume":"115 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89104423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
W. C. John, M. Ishaya, T. A. Ihum, A. Oladejo, J.O. Oyorinde, C. Ukanyirioha, O.C. Matthias
Insect pest are a major problem to food security, most especially in developing countries like Nigeria. There is an urgent need towards mitigating the effects of these group of insects, and botanical extracts had played an increasing role as an alternative insecticide. The aim of the present study is to investigate the anti-feeding effect of different dosage of Gmelina arborea stem bark and leaf powder against Dinoderus porcellus infesting yam chips. The study was conducted at the Entomology Laboratory of Federal College of Forestry, Jos. Soxhlet extraction using methanol was used to extract plant part samples. Presence of phytochemicals contained in the plant were examined following standard protocols. The antifeedant effects of different dosage of Gmelina arborea leaf and stem bark powder on Dinoderus porcellus was determined by monitoring yam chips weight loss. Data collected was subjected to analysis of variance (ANOVA) at 1 % level of significance and the means separated using Duncan test. The results revealed presence of tannin, flavoid, saponins, alkaloids, reducing sugar and phenol with absence of steroid. The results also revealed that the various dosages have remarkable antifeedant effect on the development of D. porcellus. The weight loss of treated yam chips was significantly different from those of untreated yam chips. Therefore, the results obtained in this work accounts for the use of G. arborea plant parts powders as bio-preservative for yam chips against D. porcellus.
{"title":"Pesticide Potential of Gmelina arborea Stem Bark and Leaf Powder against","authors":"W. C. John, M. Ishaya, T. A. Ihum, A. Oladejo, J.O. Oyorinde, C. Ukanyirioha, O.C. Matthias","doi":"10.4314/njb.v38i2.4","DOIUrl":"https://doi.org/10.4314/njb.v38i2.4","url":null,"abstract":"Insect pest are a major problem to food security, most especially in developing countries like Nigeria. There is an urgent need towards mitigating the effects of these group of insects, and botanical extracts had played an increasing role as an alternative insecticide. The aim of the present study is to investigate the anti-feeding effect of different dosage of Gmelina arborea stem bark and leaf powder against Dinoderus porcellus infesting yam chips. The study was conducted at the Entomology Laboratory of Federal College of Forestry, Jos. Soxhlet extraction using methanol was used to extract plant part samples. Presence of phytochemicals contained in the plant were examined following standard protocols. The antifeedant effects of different dosage of Gmelina arborea leaf and stem bark powder on Dinoderus porcellus was determined by monitoring yam chips weight loss. Data collected was subjected to analysis of variance (ANOVA) at 1 % level of significance and the means separated using Duncan test. The results revealed presence of tannin, flavoid, saponins, alkaloids, reducing sugar and phenol with absence of steroid. The results also revealed that the various dosages have remarkable antifeedant effect on the development of D. porcellus. The weight loss of treated yam chips was significantly different from those of untreated yam chips. Therefore, the results obtained in this work accounts for the use of G. arborea plant parts powders as bio-preservative for yam chips against D. porcellus.","PeriodicalId":19168,"journal":{"name":"Nigerian Journal of Biotechnology","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73038621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. C. Onyia, E. Onyeneke, T. Okunwaye, E. Okogbenin, N. U. Asiriuwa, J. U. Obibuzor, H. Anemene
Numerous metal ions and chemical compounds can influence the activity of some fungi. Some have inhibitory effects whereas others have enhancing effects. In this study, the effect of metallic chlorides; lead II chloride (PbCl2), potassium chloride (KCl), copper II chloride (CuCl2), calcium II chloride (CaCl2), manganese II chloride (MnCl2), iron III chloride (FeCl3) and mercury II chloride (HgCl2) were evaluated for their influence on the cellulolytic activity of seven (7) fungal isolates at a concentration of 0.005M at 30°C for four (4) days using 3, 5-dinitrosalicyclic acid (DNS) method. CaCl2 had the highest influence on the cellulolytic activity for all the fungal isolates as compared to the control. In the presence of CaCl2, Trichoderma species (C) showed a high cellulolytic activity of 2.28x10-5 mmol/min/ml, Rhizopus, Trichoderma and Aspergillus species (A, B and E) had activities of 1.72 x10-5 ,1.65 x10-5 and 1.68 x10-5 mmol/min/ml respectively. HgCl2 and CuCl2 had the least influenced on the cellulolytic activity of all the fungal isolates. This, therefore, suggests that calcium ion activates the cellulase activity of the fungal species while HgCl2, PbCl2, KCl, CuCl2, MnCl2 and FeCl3 had a retarding effect on the cellulolytic activity.
{"title":"The Influence Of Metal Ions On Cellulolytic Activities Of Fungal Isolates From Palm Biomass","authors":"D. C. Onyia, E. Onyeneke, T. Okunwaye, E. Okogbenin, N. U. Asiriuwa, J. U. Obibuzor, H. Anemene","doi":"10.4314/njb.v38i2.7","DOIUrl":"https://doi.org/10.4314/njb.v38i2.7","url":null,"abstract":"Numerous metal ions and chemical compounds can influence the activity of some fungi. Some have inhibitory effects whereas others have enhancing effects. In this study, the effect of metallic chlorides; lead II chloride (PbCl2), potassium chloride (KCl), copper II chloride (CuCl2), calcium II chloride (CaCl2), manganese II chloride (MnCl2), iron III chloride (FeCl3) and mercury II chloride (HgCl2) were evaluated for their influence on the cellulolytic activity of seven (7) fungal isolates at a concentration of 0.005M at 30°C for four (4) days using 3, 5-dinitrosalicyclic acid (DNS) method. CaCl2 had the highest influence on the cellulolytic activity for all the fungal isolates as compared to the control. In the presence of CaCl2, Trichoderma species (C) showed a high cellulolytic activity of 2.28x10-5 mmol/min/ml, Rhizopus, Trichoderma and Aspergillus species (A, B and E) had activities of 1.72 x10-5 ,1.65 x10-5 and 1.68 x10-5 mmol/min/ml respectively. HgCl2 and CuCl2 had the least influenced on the cellulolytic activity of all the fungal isolates. This, therefore, suggests that calcium ion activates the cellulase activity of the fungal species while HgCl2, PbCl2, KCl, CuCl2, MnCl2 and FeCl3 had a retarding effect on the cellulolytic activity.","PeriodicalId":19168,"journal":{"name":"Nigerian Journal of Biotechnology","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75660179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Edeh, E. Otto, N.E. Richard-Nnabu, S. G. Ugboaja, C. Umoke, D. Omachi
Science and technology have become essential components of healthcare. The emergence of Artificial intelligence (AI), Blockhain, mobile techs, Internet of Things (IoT) and Semantic Web Technologies (SWTs) etcetera have amplified the growing influence of technology in global health systems. IoT and SWTs in particular are seen as the next-generation technologies that can revolutionize global health systems. Thus, this paper examines the various potentials of IoT and SWTs in the health sector, with a view to creating more awareness regarding its prospects and associated risks, and also to assist stakeholders to maximize its possibilities to enrich healthcare delivery. We deduced that both IoT and SWTs harbour promising features that are now being deployed in the health sector to enhance quality service delivery, medical research and innovations, smart health, telemedicine, communications, patient care and effective health outcomes just to mention but a few. We conclude that the adoption and implementation of IoT-based devices and SWTs will be significant in sustainability and improvement of healthcare systems in the future. However, privacy and medical data security remains a major source of concern to many stakeholders.
{"title":"Potential of Internet of Things and Semantic Web Technologies in the Health Sector","authors":"M. Edeh, E. Otto, N.E. Richard-Nnabu, S. G. Ugboaja, C. Umoke, D. Omachi","doi":"10.4314/njb.v38i2.8","DOIUrl":"https://doi.org/10.4314/njb.v38i2.8","url":null,"abstract":"Science and technology have become essential components of healthcare. The emergence of Artificial intelligence (AI), Blockhain, mobile techs, Internet of Things (IoT) and Semantic Web Technologies (SWTs) etcetera have amplified the growing influence of technology in global health systems. IoT and SWTs in particular are seen as the next-generation technologies that can revolutionize global health systems. Thus, this paper examines the various potentials of IoT and SWTs in the health sector, with a view to creating more awareness regarding its prospects and associated risks, and also to assist stakeholders to maximize its possibilities to enrich healthcare delivery. We deduced that both IoT and SWTs harbour promising features that are now being deployed in the health sector to enhance quality service delivery, medical research and innovations, smart health, telemedicine, communications, patient care and effective health outcomes just to mention but a few. We conclude that the adoption and implementation of IoT-based devices and SWTs will be significant in sustainability and improvement of healthcare systems in the future. However, privacy and medical data security remains a major source of concern to many stakeholders.","PeriodicalId":19168,"journal":{"name":"Nigerian Journal of Biotechnology","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82968871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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