The study was carried out for six (6) weeks at the Department of Fisheries Teaching and Research farm, Modibbo Adama University, Yola, to compare the reproductive performance of Clarias gariepinus using synthetic (ovaprim) and natural (C. gariepinus pituitary extract) hormones . At the end of the feeding trial, the fecundity, percentage fertilization, hatchability, and survival rates of the hatchlings were estimated using standard procedure. The hatchlings were further raised for six weeks and the increase in weight was determined. The results showed that female broodstock injected with C. gariepinus pituitary extract (CgPE) had a higher fecundity (124,000) while the female injected with Ovaprim had 65,000 eggs. The weight of the testes significantly differed (p<0.05) across the treatments, the right lobe weighed 1.63g while the left lobe weighed 1.98g . Subsequently, a higher fertilization rate was recorded in CgPE (91.1%) while Ovaprim had 85.55% . However, Ovaprim had a higher percentage hatchability of 82.93% while CgPE had 82.73% . At the end of the six (6) weeks feeding period, the survival rate was higher in the fish treated with CgPE (82.31%) while the fish injected with Ovaprim had 79.68% . The research exposed the efficacy of using CgPE in the absence of synthetic hormone, CgPE is a preferred substitute that Hatchery managers can utilize for fingerlings production.
{"title":"Comparative Study on the Natural and Synthetic Hormones ofClarias gariepinus Broodstock","authors":"L. Onyia, H. Ali, H. Bello, E. Onyia, M. Musa","doi":"10.4314/njb.v38i1.5","DOIUrl":"https://doi.org/10.4314/njb.v38i1.5","url":null,"abstract":"The study was carried out for six (6) weeks at the Department of Fisheries Teaching and Research farm, Modibbo Adama University, Yola, to compare the reproductive performance of Clarias gariepinus using synthetic (ovaprim) and natural (C. gariepinus pituitary extract) hormones . At the end of the feeding trial, the fecundity, percentage fertilization, hatchability, and survival rates of the hatchlings were estimated using standard procedure. The hatchlings were further raised for six weeks and the increase in weight was determined. The results showed that female broodstock injected with C. gariepinus pituitary extract (CgPE) had a higher fecundity (124,000) while the female injected with Ovaprim had 65,000 eggs. The weight of the testes significantly differed (p<0.05) across the treatments, the right lobe weighed 1.63g while the left lobe weighed 1.98g . Subsequently, a higher fertilization rate was recorded in CgPE (91.1%) while Ovaprim had 85.55% . However, Ovaprim had a higher percentage hatchability of 82.93% while CgPE had 82.73% . At the end of the six (6) weeks feeding period, the survival rate was higher in the fish treated with CgPE (82.31%) while the fish injected with Ovaprim had 79.68% . The research exposed the efficacy of using CgPE in the absence of synthetic hormone, CgPE is a preferred substitute that Hatchery managers can utilize for fingerlings production.","PeriodicalId":19168,"journal":{"name":"Nigerian Journal of Biotechnology","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75293293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Poultry eggs are perishable, hence could lose their quality rapidly between storage and consumption. Maintaining fresh quality is a major problem which is aggravated by temperature, humidity, storage method and time. Several methods of altering these environmental conditions to prolong the shelf-life of the eggs have been reported, yet little is known about kiln, solar and oven methods of drying boiled fowl eggs. This study was targeted at creating awareness, evaluating the organoleptic properties and determining the shelf-life of kiln-, solar- and oven-dried fowl eggs, stored under different environmental conditions. A total of 160 Isa brown freshly laid eggs were obtained and weighed, and were divided into four different groups of 40 eggs each, designated as control, kiln-, solar- and oven-dried. The eggs were boiled for 30 minutes and dried at 100 – 120oC overnight (kiln dried), 45 – 50oC for 5 days (solar dried) and 100oC for 24 hours (oven dried), but those in the control group were not dried. Panelists were used in obtaining information on awareness of fowl eggs drying/drying methods and organoleptic evaluation. Twenty-seven eggs each from the control, kiln-, solar- and oven-dried groups were kept in locally fabricated egg packs and stored for 30 days in a sales outlet, deep freezer and kitchen for shelf-life determination. Results showed that egg weight loss was highest (52.3%) in the kiln-dried, followed by oven-dried (38.9%), solar-dried (27.4%) and control (20.6%) groups. All the panelists (100%) were aware of the different forms of storing boiled fowl eggs and have eaten fowl eggs in different forms, but none (0.0%) was aware of kiln, solar and oven method of drying fowl eggs, or has eaten kiln-, solar- and oven-dried fowl eggs before then. Some of the panelists liked the appearance, taste, aroma, mouth feel, after-taste and overall acceptability of the dried eggs. All (100%) of the eggs in the sales outlet spoilt, but none (0.0%) among the dried eggs stored in the deep freezer was spoilt. In the kitchen, all the control eggs (100%), 22.2% (kiln dried), 88.9% (solar dried) and 66.7% (oven dried) spoilt. Kiln- and oven-dried eggs had more water loss, apparently implying better effectiveness of the drying methods. Therefore, fowl eggs could be boiled, dried and refrigerated to elongate their shelf-lives.
{"title":"Organoleptic properties and shelf-lives of differently dried fowl eggs","authors":"K. Idahor","doi":"10.4314/njb.v38i1.14","DOIUrl":"https://doi.org/10.4314/njb.v38i1.14","url":null,"abstract":"Poultry eggs are perishable, hence could lose their quality rapidly between storage and consumption. Maintaining fresh quality is a major problem which is aggravated by temperature, humidity, storage method and time. Several methods of altering these environmental conditions to prolong the shelf-life of the eggs have been reported, yet little is known about kiln, solar and oven methods of drying boiled fowl eggs. This study was targeted at creating awareness, evaluating the organoleptic properties and determining the shelf-life of kiln-, solar- and oven-dried fowl eggs, stored under different environmental conditions. A total of 160 Isa brown freshly laid eggs were obtained and weighed, and were divided into four different groups of 40 eggs each, designated as control, kiln-, solar- and oven-dried. The eggs were boiled for 30 minutes and dried at 100 – 120oC overnight (kiln dried), 45 – 50oC for 5 days (solar dried) and 100oC for 24 hours (oven dried), but those in the control group were not dried. Panelists were used in obtaining information on awareness of fowl eggs drying/drying methods and organoleptic evaluation. Twenty-seven eggs each from the control, kiln-, solar- and oven-dried groups were kept in locally fabricated egg packs and stored for 30 days in a sales outlet, deep freezer and kitchen for shelf-life determination. Results showed that egg weight loss was highest (52.3%) in the kiln-dried, followed by oven-dried (38.9%), solar-dried (27.4%) and control (20.6%) groups. All the panelists (100%) were aware of the different forms of storing boiled fowl eggs and have eaten fowl eggs in different forms, but none (0.0%) was aware of kiln, solar and oven method of drying fowl eggs, or has eaten kiln-, solar- and oven-dried fowl eggs before then. Some of the panelists liked the appearance, taste, aroma, mouth feel, after-taste and overall acceptability of the dried eggs. All (100%) of the eggs in the sales outlet spoilt, but none (0.0%) among the dried eggs stored in the deep freezer was spoilt. In the kitchen, all the control eggs (100%), 22.2% (kiln dried), 88.9% (solar dried) and 66.7% (oven dried) spoilt. Kiln- and oven-dried eggs had more water loss, apparently implying better effectiveness of the drying methods. Therefore, fowl eggs could be boiled, dried and refrigerated to elongate their shelf-lives.","PeriodicalId":19168,"journal":{"name":"Nigerian Journal of Biotechnology","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78108401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Ezaka, O. Nchedo, E. N. Ugbo, A. Adediran, O. Ayanda
Yeast is one of the organisms that grow and survive in diverse conditions. The influence of environmental factors on its growth and proliferation were evaluated. The yeast used for the study was isolated from honey using Sabouraud Dextrose Agar and pour plate methods. The effects of temperature, pH and ultraviolet radiation on the growth of Rhodotorula, Debaryomyces, Zygosaccharomyces and Candida species were determined using standard methods. The growths of all the yeast isolates were greatly affected at 80oC. Rhodotorulla species showed least growth at 50oC, but optimal growth was recorded at 30oC after 96 h of incubation. A similar trend was observed in Debaromyces species. Zygosaccharomyces species recorded high reduction in growth at 60oC after 96 h of incubation, though there were no significant differences in the growths of the isolates at 60oC and 80oC. Candida species recorded the least effect of temperature at 30oC and highest at 60oC and 80oC after 96 h of incubation. The results of the effect of pH on growth of the yeasts showed that Rhodotorula and Zygosaccharomyces had optimum growth at pH of 5 and 4 respectively. All the isolates showed increase in growth with increase in incubation time. Exposure of the isolates to UV-rays negatively affected the growths of the isolates. The growths of the yeasts decreased with increase in exposure time. This study showed that yeast can survive adverse temperature and pH and can as well survive UV-ray exposure.
{"title":"Effects of Environmental factors on the Growth and Proliferation of Yeasts","authors":"E. Ezaka, O. Nchedo, E. N. Ugbo, A. Adediran, O. Ayanda","doi":"10.4314/njb.v38i1.20","DOIUrl":"https://doi.org/10.4314/njb.v38i1.20","url":null,"abstract":"Yeast is one of the organisms that grow and survive in diverse conditions. The influence of environmental factors on its growth and proliferation were evaluated. The yeast used for the study was isolated from honey using Sabouraud Dextrose Agar and pour plate methods. The effects of temperature, pH and ultraviolet radiation on the growth of Rhodotorula, Debaryomyces, Zygosaccharomyces and Candida species were determined using standard methods. The growths of all the yeast isolates were greatly affected at 80oC. Rhodotorulla species showed least growth at 50oC, but optimal growth was recorded at 30oC after 96 h of incubation. A similar trend was observed in Debaromyces species. Zygosaccharomyces species recorded high reduction in growth at 60oC after 96 h of incubation, though there were no significant differences in the growths of the isolates at 60oC and 80oC. Candida species recorded the least effect of temperature at 30oC and highest at 60oC and 80oC after 96 h of incubation. The results of the effect of pH on growth of the yeasts showed that Rhodotorula and Zygosaccharomyces had optimum growth at pH of 5 and 4 respectively. All the isolates showed increase in growth with increase in incubation time. Exposure of the isolates to UV-rays negatively affected the growths of the isolates. The growths of the yeasts decreased with increase in exposure time. This study showed that yeast can survive adverse temperature and pH and can as well survive UV-ray exposure.","PeriodicalId":19168,"journal":{"name":"Nigerian Journal of Biotechnology","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83714924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. I. Woghiren, O. AwodoyinR., C. I. Antiabong, E. N. Ngonadi, O. Jeminiwa, S. Olaoti-Laaro
Weed interference is a major constraint in maize cultivation. Living mulch as an alternative weed control strategy has been established to be environmentally safe but has not been widely used in maize cultivation. The aim of this research was to evaluate the weed management attributes of Vigna unguiculata in maize cropping. A field study was carried out in the crop garden of the Department of Crop Protection and Environmental Biology, University of Ibadan, Nigeria. The treatments were maize interplanted with Cowpea at 20,000 (M1), 30,000 (M2), 40,000 (M3) plants/hectare, hoe weeding (M4), weedy check (M5) and Primextra-2.5 L/ha (M6). The treatments were arranged in randomized complete block design, each replicated four times. Weed Dry Weight (WDW) and Weed Control Efficiency–WCE (%) were calculated following standardized methods. Data were analysed using descriptive statistics and ANOVA at α0.05. The treatment plots were dominated by weed species in the Asteraceae, Fabaceae and Poaceae families. The M5 accounted for the highest WDW (126.30 g). The WCE was highest in M3 (94.8%) and least in M5 (66.4%). Maize and cowpea interplant at 40,000 plants/hectare suppressed weed. Hence, cowpea is an ideal weed suppressant and can be inter-planted as a cover crop in maize cropping systems.
{"title":"Effects of cowpea (Vigna unguiculata L. Walp.) as a Live mulch on weed management in maize cropping","authors":"A. I. Woghiren, O. AwodoyinR., C. I. Antiabong, E. N. Ngonadi, O. Jeminiwa, S. Olaoti-Laaro","doi":"10.4314/njb.v38i1.7","DOIUrl":"https://doi.org/10.4314/njb.v38i1.7","url":null,"abstract":"Weed interference is a major constraint in maize cultivation. Living mulch as an alternative weed control strategy has been established to be environmentally safe but has not been widely used in maize cultivation. The aim of this research was to evaluate the weed management attributes of Vigna unguiculata in maize cropping. A field study was carried out in the crop garden of the Department of Crop Protection and Environmental Biology, University of Ibadan, Nigeria. The treatments were maize interplanted with Cowpea at 20,000 (M1), 30,000 (M2), 40,000 (M3) plants/hectare, hoe weeding (M4), weedy check (M5) and Primextra-2.5 L/ha (M6). The treatments were arranged in randomized complete block design, each replicated four times. Weed Dry Weight (WDW) and Weed Control Efficiency–WCE (%) were calculated following standardized methods. Data were analysed using descriptive statistics and ANOVA at α0.05. The treatment plots were dominated by weed species in the Asteraceae, Fabaceae and Poaceae families. The M5 accounted for the highest WDW (126.30 g). The WCE was highest in M3 (94.8%) and least in M5 (66.4%). Maize and cowpea interplant at 40,000 plants/hectare suppressed weed. Hence, cowpea is an ideal weed suppressant and can be inter-planted as a cover crop in maize cropping systems.","PeriodicalId":19168,"journal":{"name":"Nigerian Journal of Biotechnology","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84554709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In many parts of Africa especially in Nigeria, ready-to-eat rice is commonly sold in several locations within the big cities and villages. In this study, one hundred samples of ready-to-eat rice were analyzed in some selected areas in Abeokuta, Ogun State, Nigeria. The effects of wrapping materials on the microbial quality of the rice samples were determined from seven different locations. The total bacterial counts ranged from 5.66 to 7.30 log10 cfu/g ,total coliform counts ranged from 5.07 to 7.33 log10 cfu/g and total fungal counts ranged from 5.19 to 6.03 log10 cfu/g. The b acterial species isolated from these samples were Bacillus subtilis, Klebsiella pneumonia and Staphylococcus aureus while the fungal species isolated include Aspergillus fumigatus, Mucor spp., and Penicillium sp. Factors such as improper storage as well as handling of the wrapping-materials of the food coupled with the attitude and practices of the vendors are likely to be responsible for the growth of these microorganisms. It was also discovered that improper cooking may contribute to microbial growth. Provision and enforcement of strict hygienic practices would go a long way in improving the quality of the ready-to-eat foods.
{"title":"Effect of Wrapping Materials on the Microbial Quality of some Street Vended Ready- to - Eat Rice","authors":"C. O. Ojesola, O. Afolabi, A. Oloyede","doi":"10.4314/njb.v38i1.6","DOIUrl":"https://doi.org/10.4314/njb.v38i1.6","url":null,"abstract":"In many parts of Africa especially in Nigeria, ready-to-eat rice is commonly sold in several locations within the big cities and villages. In this study, one hundred samples of ready-to-eat rice were analyzed in some selected areas in Abeokuta, Ogun State, Nigeria. The effects of wrapping materials on the microbial quality of the rice samples were determined from seven different locations. The total bacterial counts ranged from 5.66 to 7.30 log10 cfu/g ,total coliform counts ranged from 5.07 to 7.33 log10 cfu/g and total fungal counts ranged from 5.19 to 6.03 log10 cfu/g. The b acterial species isolated from these samples were Bacillus subtilis, Klebsiella pneumonia and Staphylococcus aureus while the fungal species isolated include Aspergillus fumigatus, Mucor spp., and Penicillium sp. Factors such as improper storage as well as handling of the wrapping-materials of the food coupled with the attitude and practices of the vendors are likely to be responsible for the growth of these microorganisms. It was also discovered that improper cooking may contribute to microbial growth. Provision and enforcement of strict hygienic practices would go a long way in improving the quality of the ready-to-eat foods.","PeriodicalId":19168,"journal":{"name":"Nigerian Journal of Biotechnology","volume":"139 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76732450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Okunwaye, P. Uadia, B. O. Okogbenin, E. Okogbenin, D. C. Onyia, J. U. Obibuzor
Amylases are enzymes that catalyze the hydrolysis of glycosidic bonds present in starch to release simple sugars. They are one of the most important enzymes in numerous commercial processes. In this investigation, fungal and bacterial strains from the following agro-industrial wastes were isolated and screened for amylolytic ability: soil from oil palm plantation, shea seed, date fruit, coconut meat, cassava effluent, cassava peel, cassava tubers, yam and potato tubers, starch medium, parboiled water from noodles and rice. The results revealed the presence of Geotrichum, Aspergillus, Penicillium, Trichoderma, Rhizopus and Fusarium spp. Five major genera of bacterial species namely Corynebacterium, Pseudomonas, Lactobacillus, Micrococcus and Bacillus were isolated and screened for amylase activity. Cassava soil had the highest heterotrophic bacterial count of 5.7 x105cfu/g and coconut meat waste had the lowest heterotrophic bacterial count of 1.3 x105cfu/g. All isolated microorganisms had the amylolytic ability. The fungal isolates had higher amylase activity when compared with the bacterial isolates. This investigation reveals organisms with high amylase activity.
{"title":"Amylase-Producing Fungi and Bacteria Associated with Some Food Processing Wastes","authors":"T. Okunwaye, P. Uadia, B. O. Okogbenin, E. Okogbenin, D. C. Onyia, J. U. Obibuzor","doi":"10.4314/njb.v38i1.9","DOIUrl":"https://doi.org/10.4314/njb.v38i1.9","url":null,"abstract":"Amylases are enzymes that catalyze the hydrolysis of glycosidic bonds present in starch to release simple sugars. They are one of the most important enzymes in numerous commercial processes. In this investigation, fungal and bacterial strains from the following agro-industrial wastes were isolated and screened for amylolytic ability: soil from oil palm plantation, shea seed, date fruit, coconut meat, cassava effluent, cassava peel, cassava tubers, yam and potato tubers, starch medium, parboiled water from noodles and rice. The results revealed the presence of Geotrichum, Aspergillus, Penicillium, Trichoderma, Rhizopus and Fusarium spp. Five major genera of bacterial species namely Corynebacterium, Pseudomonas, Lactobacillus, Micrococcus and Bacillus were isolated and screened for amylase activity. Cassava soil had the highest heterotrophic bacterial count of 5.7 x105cfu/g and coconut meat waste had the lowest heterotrophic bacterial count of 1.3 x105cfu/g. All isolated microorganisms had the amylolytic ability. The fungal isolates had higher amylase activity when compared with the bacterial isolates. This investigation reveals organisms with high amylase activity.","PeriodicalId":19168,"journal":{"name":"Nigerian Journal of Biotechnology","volume":"176 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88466954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study examined antifungal efficacy of Vitellaria paradoxa fractions and compounds in the control of some wood degrading fungi. Stem bark and heartwood parts of Vitellaria paradoxa were collected, dried, pulverised and macerated sequentially in n- hexane, methanol and ethyl acetate solvents. The mixtures were filtered, evaporated and the dried samples were mixed and run over silica gel in column chromatography with a mixture of n -hexane and ethyl acetate solvents to obtain fractions. The fractions collected were evaporated and those with white needles were subjected to Magnetic Resonance (NMR) spectroscopic analysis. Spinasterol was isolated and characterised from the heartwood fraction while the stem bark fractions were fatty. Vitellaria paradoxa fractions were active against Serpula lacrymans, Sclerotium rolfsii, Aspergillus fumigatus, Fomitopsis pinicoca, Phaeolus schweinitzii, Rhizopus sp., Coniophora puteana, Gloeophyllum sepiarium, and Fibroporia vaillantii at zones of inhibition (ZOI) of 18 mm - 24 mm. Although the antibiotics were active (25 – 31 mm), they were found inactive against the Fomitopsis pinicoca fungus which was sensitive to all the V. paradoxa fractions at zones of inhibition of 18 - 24 mm. The minimum inhibitory concentrations (MIC) of the V. paradoxa fractions were active at 50 μg/mL against all test fungi. At minimum fungicidal concentration (MFC) of between 50 - 200 μg/mL, all the test fungi were killed. Based on the ZOI, MIC and MFC, the V. paradoxa stem bark heartwood fractions have been proven to be very efficient in inhibiting the growth of test wood rot fungi; hence the species could be explored as a potential source of bioactive fungicides.
{"title":"Antimicrobial Efficacy of Vitellaria paradoxa fractions and compounds on some wood Fungi and Bacteria","authors":"D. Ekhuemelo, J. Anyam, C. Ekhuemelo","doi":"10.4314/njb.v38i1.1","DOIUrl":"https://doi.org/10.4314/njb.v38i1.1","url":null,"abstract":"This study examined antifungal efficacy of Vitellaria paradoxa fractions and compounds in the control of some wood degrading fungi. Stem bark and heartwood parts of Vitellaria paradoxa were collected, dried, pulverised and macerated sequentially in n- hexane, methanol and ethyl acetate solvents. The mixtures were filtered, evaporated and the dried samples were mixed and run over silica gel in column chromatography with a mixture of n -hexane and ethyl acetate solvents to obtain fractions. The fractions collected were evaporated and those with white needles were subjected to Magnetic Resonance (NMR) spectroscopic analysis. Spinasterol was isolated and characterised from the heartwood fraction while the stem bark fractions were fatty. Vitellaria paradoxa fractions were active against Serpula lacrymans, Sclerotium rolfsii, Aspergillus fumigatus, Fomitopsis pinicoca, Phaeolus schweinitzii, Rhizopus sp., Coniophora puteana, Gloeophyllum sepiarium, and Fibroporia vaillantii at zones of inhibition (ZOI) of 18 mm - 24 mm. Although the antibiotics were active (25 – 31 mm), they were found inactive against the Fomitopsis pinicoca fungus which was sensitive to all the V. paradoxa fractions at zones of inhibition of 18 - 24 mm. The minimum inhibitory concentrations (MIC) of the V. paradoxa fractions were active at 50 μg/mL against all test fungi. At minimum fungicidal concentration (MFC) of between 50 - 200 μg/mL, all the test fungi were killed. Based on the ZOI, MIC and MFC, the V. paradoxa stem bark heartwood fractions have been proven to be very efficient in inhibiting the growth of test wood rot fungi; hence the species could be explored as a potential source of bioactive fungicides.","PeriodicalId":19168,"journal":{"name":"Nigerian Journal of Biotechnology","volume":"68 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83713197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A total of twenty-five crossbred rabbit bucks with a mean weight of 628.73g were utilized to investigate their nutrient digestibility, visceral organ changes, and carcass yield when fed with diets containing graded levels of rumen filtrate-fermented rice offal (FRO) for 10 weeks. Five rabbits were grouped into five treatments with each rabbit serving as a replicate in a completely randomized design experiment. The control group was fed with a diet containing no rumen filtrate fermented-rice offal meal while the other four groups were fed with diets in which the FRO replaced the maize at 5%, 10%, 15%, and 20%, respectively. The results of the analyzed nutrient composition and energy content of the experimental diets showed no trend across the treatments except for crude protein and metabolizable energy whose values appeared to increase across treatments as the levels of FRO increased from 0% to 20%. Dry Matter (DM), Crude Fiber (CF), Ether Extract (EE) and Nitrogen Free Extract (NFE) digestibility values were affected by the inclusion level of the fermented rice offal in the diets of the rabbits, except Crude Protein (CP). Crude fiber values appeared to increase across the treatments as the levels of FRO increased from 0% to 20%. Dressed weight showed significantly (p<0.05) higher values for rabbits fed with diets containing 20% FRO and prime cuts were significantly (p<0.05) influenced by treatments. The result of visceral organ and GIT weights showed treatment effect (p<0.05), except fasted live weights, spleen, and visceral fats. Of the visceral organ lengths, only the esophagus and stomach were similar (p>0.05) across treatment groups. It was therefore concluded that the digestibility of nutrients was not adversely affected; such that 20% inclusion of rumen filtrate-fermented rice offal showed no modification on visceral organ changes and a better carcass yield observed in rabbits on the diets. It was suggested that up to 20% rumen filtrate-fermented rice offal meal can be integrated into rabbit diets without impairing their digestibility, visceral organ characteristics, and carcass yield.
{"title":"Nutrient digestibility, visceral organ changes and carcass yield of rabbits fed with diets containing graded levels of rumen filtrate-fermented rice offal","authors":"A. T. Kpehe, C. D. Tuleun, F. G. Kaankuka","doi":"10.4314/njb.v38i1.2","DOIUrl":"https://doi.org/10.4314/njb.v38i1.2","url":null,"abstract":"A total of twenty-five crossbred rabbit bucks with a mean weight of 628.73g were utilized to investigate their nutrient digestibility, visceral organ changes, and carcass yield when fed with diets containing graded levels of rumen filtrate-fermented rice offal (FRO) for 10 weeks. Five rabbits were grouped into five treatments with each rabbit serving as a replicate in a completely randomized design experiment. The control group was fed with a diet containing no rumen filtrate fermented-rice offal meal while the other four groups were fed with diets in which the FRO replaced the maize at 5%, 10%, 15%, and 20%, respectively. The results of the analyzed nutrient composition and energy content of the experimental diets showed no trend across the treatments except for crude protein and metabolizable energy whose values appeared to increase across treatments as the levels of FRO increased from 0% to 20%. Dry Matter (DM), Crude Fiber (CF), Ether Extract (EE) and Nitrogen Free Extract (NFE) digestibility values were affected by the inclusion level of the fermented rice offal in the diets of the rabbits, except Crude Protein (CP). Crude fiber values appeared to increase across the treatments as the levels of FRO increased from 0% to 20%. Dressed weight showed significantly (p<0.05) higher values for rabbits fed with diets containing 20% FRO and prime cuts were significantly (p<0.05) influenced by treatments. The result of visceral organ and GIT weights showed treatment effect (p<0.05), except fasted live weights, spleen, and visceral fats. Of the visceral organ lengths, only the esophagus and stomach were similar (p>0.05) across treatment groups. It was therefore concluded that the digestibility of nutrients was not adversely affected; such that 20% inclusion of rumen filtrate-fermented rice offal showed no modification on visceral organ changes and a better carcass yield observed in rabbits on the diets. It was suggested that up to 20% rumen filtrate-fermented rice offal meal can be integrated into rabbit diets without impairing their digestibility, visceral organ characteristics, and carcass yield.","PeriodicalId":19168,"journal":{"name":"Nigerian Journal of Biotechnology","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80836597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
W. C. John, M. Ishaya, A. Oladejo, O. Olori-Oke, T. A. Ihum, C. Ukanyirioha
The study aims at evaluating the efficacy of different doses of powdered stem and leaf parts of Gmelina arborea on Dinoderus porcellus infesting yam chips in Jos metropolis. The experiment was conducted at the Entomology Laboratory of the Federal College of Forestry, Jos. Samples of infested yam chips and Gmelina arborea were collected in Jos metropolis. Newly emerged adult Dinoderus porcellus were exposed to the yam chips which were mixed with different levels of Gmelina arborea parts powder (0, 5, 10, 15 and 20 g). The setup was monitored for 120 hours and mortality recorded. The study was carried out in a randomized complete design with treatments replicated thrice. Data was analysed using analysis of variance at 1 and 5 percent confidence level and Duncan test was used to separate the means. The results obtained reveal significant differences at p value=0.05 and 0.01. The results reveal that in all the doses, an increase in the amount of the powder resulted in increased incidence of D. porcellus mortality. After 120 hours of exposure, 100 % mortality was recorded from either 15 or 20 g stem bark + leaf powder treatment. From the results obtained, the use of 15 g stem bark+leaf powder of Gmelina arborea could be effective in yam chips preservation which could in turn strengthen food security.
{"title":"Effects of Gmelina arborea Powdered Parts on Mortality of Dinoderus porcellus in Yam Chips","authors":"W. C. John, M. Ishaya, A. Oladejo, O. Olori-Oke, T. A. Ihum, C. Ukanyirioha","doi":"10.4314/NJB.V37I2.19","DOIUrl":"https://doi.org/10.4314/NJB.V37I2.19","url":null,"abstract":"The study aims at evaluating the efficacy of different doses of powdered stem and leaf parts of Gmelina arborea on Dinoderus porcellus infesting yam chips in Jos metropolis. The experiment was conducted at the Entomology Laboratory of the Federal College of Forestry, Jos. Samples of infested yam chips and Gmelina arborea were collected in Jos metropolis. Newly emerged adult Dinoderus porcellus were exposed to the yam chips which were mixed with different levels of Gmelina arborea parts powder (0, 5, 10, 15 and 20 g). The setup was monitored for 120 hours and mortality recorded. The study was carried out in a randomized complete design with treatments replicated thrice. Data was analysed using analysis of variance at 1 and 5 percent confidence level and Duncan test was used to separate the means. The results obtained reveal significant differences at p value=0.05 and 0.01. The results reveal that in all the doses, an increase in the amount of the powder resulted in increased incidence of D. porcellus mortality. After 120 hours of exposure, 100 % mortality was recorded from either 15 or 20 g stem bark + leaf powder treatment. From the results obtained, the use of 15 g stem bark+leaf powder of Gmelina arborea could be effective in yam chips preservation which could in turn strengthen food security.","PeriodicalId":19168,"journal":{"name":"Nigerian Journal of Biotechnology","volume":"4 1","pages":"194-202"},"PeriodicalIF":0.0,"publicationDate":"2021-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82927342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The high cost of animal protein sources has made it necessary to evaluate the use of alternative plant protein sources especially unconventional plant proteins. Leucaena leucocephala (white lead tree) is a leguminous plant, the leaves of which have the potential of being used as a plant protein source. Leucaena leaf has been analysed to have a crude protein value of 15.234.3%. The plant leaves were collected from the forage section of the College of Agriculture and Animal Sciences (CAAS) Mando, Kaduna. The first group of the leaves was divided into three and sun-dried for 24 hours, 48 hours and 72 hours respectively. The second group was further divided into three subgroups which were soaked in water for 24 hours, 48 hours and 72 hours then sun-dried. The two groups of the leaves were ground into leaf powder. The effects of sun-drying and soaking on amino acid composition of L. leucocephala leaves was investigated. The amino acid analysis was done by ion-exchange chromatography (IEC) using the Technicon Sequential Multisample Amino Acid Analyser (TSM) Technicon Instruments Corporation, New York. The essential amino acids detected in L. leucocephala leaf were arginine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine and valine. Alanine, cysteine, tyrosine, aspartic acid, glutamic acid, glycine, serine and proline were the non-essential amino acids detected. Sun-drying and soaking led to significant (P≤0.05) losses in the concentration of all the amino acids in the leaf samples (Sundried for 24 hours SD1, Sundried for 48 hours SD2, Sundried for 72 hours SD3, soaked in water for 24 hours – SW1, soaked in water for 48 hours SW2, soaked in water for 72 hours SW3) when compared with the raw leaf samples (SU). Soaking caused higher reduction in the amino acid levels when compared to sun-drying in the test leaf meals which was significant at P≤0.05. The amino acid scores revealed that lysine and methionine were the limiting amino acids in L. leucocephala leaves relative to WHO/FAO/UNU standard for preschool aged children. Therefore, processed L. leucocephala leaves should be supplemented with other feed ingredients rich in these amino acids for animal feed production.
{"title":"Effects of processing on amino acids composition of Leucaena leucocephala (Lam De Wit) leaf meal","authors":"A. Agbo","doi":"10.4314/NJB.V37I2.16","DOIUrl":"https://doi.org/10.4314/NJB.V37I2.16","url":null,"abstract":"The high cost of animal protein sources has made it necessary to evaluate the use of alternative plant protein sources especially unconventional plant proteins. Leucaena leucocephala (white lead tree) is a leguminous plant, the leaves of which have the potential of being used as a plant protein source. Leucaena leaf has been analysed to have a crude protein value of 15.234.3%. The plant leaves were collected from the forage section of the College of Agriculture and Animal Sciences (CAAS) Mando, Kaduna. The first group of the leaves was divided into three and sun-dried for 24 hours, 48 hours and 72 hours respectively. The second group was further divided into three subgroups which were soaked in water for 24 hours, 48 hours and 72 hours then sun-dried. The two groups of the leaves were ground into leaf powder. The effects of sun-drying and soaking on amino acid composition of L. leucocephala leaves was investigated. The amino acid analysis was done by ion-exchange chromatography (IEC) using the Technicon Sequential Multisample Amino Acid Analyser (TSM) Technicon Instruments Corporation, New York. The essential amino acids detected in L. leucocephala leaf were arginine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine and valine. Alanine, cysteine, tyrosine, aspartic acid, glutamic acid, glycine, serine and proline were the non-essential amino acids detected. Sun-drying and soaking led to significant (P≤0.05) losses in the concentration of all the amino acids in the leaf samples (Sundried for 24 hours SD1, Sundried for 48 hours SD2, Sundried for 72 hours SD3, soaked in water for 24 hours – SW1, soaked in water for 48 hours SW2, soaked in water for 72 hours SW3) when compared with the raw leaf samples (SU). Soaking caused higher reduction in the amino acid levels when compared to sun-drying in the test leaf meals which was significant at P≤0.05. The amino acid scores revealed that lysine and methionine were the limiting amino acids in L. leucocephala leaves relative to WHO/FAO/UNU standard for preschool aged children. Therefore, processed L. leucocephala leaves should be supplemented with other feed ingredients rich in these amino acids for animal feed production.","PeriodicalId":19168,"journal":{"name":"Nigerian Journal of Biotechnology","volume":"10 2 1","pages":"157-164"},"PeriodicalIF":0.0,"publicationDate":"2021-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78344736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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