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Determination and classification of pollutants in waste water 废水中污染物的测定和分类
Q4 Agricultural and Biological Sciences Pub Date : 2021-11-22 DOI: 10.36547/nbc.1281
Zuzana Kiralyova, Katja Šnuderl, F. Kraic, J. Mocák
This paper deals with waste waters produced by industrial producers during recent three years. Its main purpose is to evaluate the data monitored from discharges of three leather plants where eight traditional variables (COD, BOD, insoluble matters, pH, and the concentrations of ammonia, total nitrogen, chromium and sulphides) were regularly analyzed and quantified. Chemometrical and statistical methods were approved as very useful tools for characterization and classification of various kinds of water samples considering the environmental and metrological aspects. For this purpose, multivariate (multidimensional) techniques of data analysis and correlation analysis were especially very useful. The mentioned techniques are used in this work to (1) reveal the concealed interrelations of the monitored characteristics of waste waters, (2) compare the results of individual waste water producers and find the most important polluting source during a long time period, and (3) derive some generally valid conclusions regarding the observed results.
本文论述了近三年来工业生产者产生的废水。其主要目的是评估三家皮革厂排放的监测数据,定期分析和量化八个传统变量(COD、BOD、不溶物、pH以及氨、总氮、铬和硫化物的浓度)。考虑到环境和计量方面,化学计量和统计方法被批准为对各种水样进行表征和分类的非常有用的工具。为此,数据分析和相关性分析的多变量(多维)技术特别有用。在这项工作中,上述技术用于(1)揭示废水监测特征之间隐藏的相互关系,(2)比较单个废水生产者的结果,并在长时间内找到最重要的污染源,以及(3)得出一些关于观测结果的普遍有效结论。
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引用次数: 0
Conversion of corn fiber into fuel ethanol 玉米纤维转化为燃料乙醇
Q4 Agricultural and Biological Sciences Pub Date : 2021-11-22 DOI: 10.36547/nbc.1276
V. Horváthová
Corn fiber due to its chemical composition (up to 20% starch, 50 - 60% non-starch polysaccharides) and availability has potential to serve as a substrate for manufacture of various products, including fuel ethanol. This paper deals with assessment of fiber-to-ethanol conversion. The water/dry fiber ratio in suspensions was 10/1. Enzyme liquefaction and saccharification of residual starch in corn fiber was carried out in two steps with thermostable α-amylase (20 min, 120°C) and mixture of pullulanase and glucomalyse (24 hours, 60°C). Procedures resulted in release of 57.7±1.6 mg of glucose per gram of dry fiber basis. It responds to the dextrose equivalent expression to 96.7±2.2%. By fermentation of the starch hydrolysates by yeasts Saccharomyces cerevisiae CCY-11-3 (5% v/v inoculum, 28°C, 72 hours) 0.48 g of ethanol per gram of glucose in hydrolysates was obtained. The solids after starch hydrolysis were separated by filtration and processed by acid pretreatment (0.1 g of conc. HCl/g of biomass/5 ml of water, 120°C, 20 min) with subsequent enzyme hydrolysis (24 hours, 60°C) by the multienzyme preparations containing cellulases and hemicellulases. Overall yield of reducing sugars after these two steps was 740.7±3.9 mg/gram of dry corn fiber basis. Fermentation of lignocellulosic hydrolysates by yeasts Pichia stipitis CCY-39-50-1 and Candida shehatea CCY-29-68-4 (in both cases 5% v/v inoculum, 28°C, 72 hours) resulted in 0.38 and 0.12 g of ethanol per gram of reducing sugars. The results indicate that applied pretreatment methods and used microorganisms are able to produce ethanol from corn fiber.
玉米纤维由于其化学成分(高达20%的淀粉,50 - 60%的非淀粉多糖)和可用性,有潜力作为制造各种产品的底物,包括燃料乙醇。本文讨论了纤维制乙醇转化的评价。悬浮液的水干比为10/1。采用耐热α-淀粉酶(20 min, 120℃)和葡聚糖酶-糖解酶(24 h, 60℃)两步对玉米纤维中残留淀粉进行酶液化和糖化。实验结果显示,每克干纤维基释放57.7±1.6毫克葡萄糖。对葡萄糖当量表达的响应为96.7±2.2%。通过酵母Saccharomyces cerevisiae CCY-11-3 (5% v/v接种量,28°C, 72小时)发酵淀粉水解产物,每克水解产物中乙醇含量为0.48 g。淀粉水解后的固体经过滤分离,经酸预处理(0.1 g conc)处理。HCl/g生物量/5 ml水,120℃,20 min),随后用含有纤维素酶和半纤维素酶的多酶制剂进行酶水解(24小时,60℃)。这两步后的还原糖总产率为740.7±3.9 mg/g。毕赤酵母(Pichia stipitis) CCY-39-50-1和假丝酵母(Candida shehatea) CCY-29-68-4发酵木质纤维素水解物(在两种情况下,5% v/v接种量,28°C, 72小时),每克还原糖产生0.38和0.12 g乙醇。结果表明,采用预处理方法和利用的微生物能够从玉米纤维中生产乙醇。
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引用次数: 0
Chlorine production for water disinfection by the means of photovoltaic panels 利用光伏板生产氯用于水消毒
Q4 Agricultural and Biological Sciences Pub Date : 2021-11-22 DOI: 10.36547/nbc.1279
S. Hostin, Peter Benedikovič, A. Michalíková
In this contribution a possibility of electrochemical production of chlorine for water disinfection, by using photovoltaic panels from solar energy, is described. A simple way of chloride production by means of a photovoltaic panel, comparable with classical electrical power source was performed on an experimental device. By using photovoltaic panel with nominal output 50 W and solar irrigation 380 – 550 W/m2 chlorine production was 0.3 mg/min, which represents amount of chlorine sufficient for disinfections of approximately 4000 l water per day.
在这篇贡献中,描述了利用太阳能光伏板电化学生产水消毒用氯的可能性。在实验装置上进行了一种可与传统电源相媲美的光电板生产氯化物的简单方法。通过使用标称输出功率为50 W的光伏板和380 - 550 W/m2的太阳能灌溉,氯产量为0.3 mg/min,这代表了每天约4000升水的消毒氯量。
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引用次数: 0
The possibility to enhance flavonoids production in Rubia tinctorum L. callus cultures 提高紫檀愈伤组织黄酮类化合物产量的可能性
Q4 Agricultural and Biological Sciences Pub Date : 2021-11-22 DOI: 10.36547/nbc.1277
D. Kákoniová, E. Űrgeová, Zuzana Juráková
Production of flavonoids in madder callus culture (Rubia tinctorum L.) was dependent on culture conditions and culture media composition. The content of flavonoids increased in calli maintained on media supplemented with NAA (4 mg.l-1) or NAA:BAP (4 mg.l-1 a 1 mg.l-1) in 16 h photoperiod. Flavonoids represented 2.08 – 2.25 % of callus dry mass. The presence of Cd(NO3)2 (3.1 or 31.0 mg.l-1 concentrations) negatively influenced callus growth, but enhaced the percentage of dry mass in callus cells. During 42 days of culture an increase of cadmium accumulation and even of flavonoids has been observed. The most considerable influence of CdCl2 or Cd(NO3)2 on flavonoids content has been shown in short-term experiments after 48 h of callus culture. More distinct influence has been observed under the treatment with CdCl2 (0.005 mg.l-1) in comparison with Cd(NO3)2.
茜草愈伤组织黄酮类化合物的产生受培养条件和培养基组成的影响。在添加NAA (4 mg.l-1)或NAA:BAP (4 mg)培养基上维持的愈伤组织中黄酮类化合物含量增加。L-1 (1 mg.l-1), 16 h光周期。黄酮类化合物占愈伤组织干质量的2.08 ~ 2.25%。Cd(NO3)2(3.1或31.0 mg)的存在。L-1浓度对愈伤组织生长有负面影响,但对愈伤组织细胞干质量百分比有提高作用。在42 d的培养过程中,观察到镉的积累增加,甚至黄酮类化合物的积累也增加。CdCl2和Cd(NO3)2对愈伤组织培养48 h后黄酮类化合物含量的影响最为显著。与Cd(NO3)2相比,CdCl2 (0.005 mg.l-1)处理下观察到更明显的影响。
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引用次数: 2
Comparison of ten biochemical laboratory tests before and after treatment by statins 他汀类药物治疗前后10项生化实验室指标的比较
Q4 Agricultural and Biological Sciences Pub Date : 2021-11-22 DOI: 10.36547/nbc.1215
T. Ďurčeková, J. Mocák, J. Balla, Gabriela Gromanová, K. Boronová
Results of 10 biochemical tests of 172 patient data (among them 84 men data and 88 women data, resp.) before and after administration of statins were thoroughly studied. All monitored patients are characterized by disorders of lipoprotein metabolism or other kind of dislipidaemia. The calculations were performed using four chemometrical methods facilitating quantification and visualization of the statin effect upon most important biochemical parameters, mainly lipid markers, and allowing classification of the patient blood samples taking into account whether the patient has been or has not been medicated by a statin drug.
对172例患者资料(其中男性84例,女性88例)给予他汀类药物前后的10项生化检查结果进行了全面研究。所有监测的患者均以脂蛋白代谢紊乱或其他类型的二脂血症为特征。使用四种化学计量学方法进行计算,便于量化和可视化他汀类药物对最重要生化参数(主要是脂质标志物)的影响,并允许根据患者是否服用过他汀类药物对患者血液样本进行分类。
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引用次数: 0
Cr and Ni simultaneous phytotoxicity and mutagenicity assay 铬和镍同时植物毒性和致突变性试验
Q4 Agricultural and Biological Sciences Pub Date : 2021-11-22 DOI: 10.36547/nbc.1156
A. Fargašová, Jana Lištiaková
For genotoxicity study simultaneous phytotoxicity and mutagenicity assay with Vicia sativa L. var. Klára was used. For phytotoxicity the following rank orders of growth inhibition can be arranged: for roots: Ni(II) > Cr(VI) > Cr(III); for shoots: Ni(II) > Cr(VI) ≥ Cr (III). For mutagenicity assay root tips of V. sativa were used and chromosome aberrations were determined at least in 500-anatelophases. All tested metals exerted in V. sativa a significant increase of chromosomal aberration rate in applied concentrations. Maximum of aberrations invoked Cr(VI) and the rank order of aberrations fall was: Cr(VI) > Ni(II) > Cr(III). Genotoxic effects of metals were determined by analysis of micronuclei frequency in the pollen tetrads of Tradescantia plants. None of tested metal significantly stimulated micronuclei frequency and genotoxic effect was decreased in order: Cr(VI) ≥ Ni(II) > Cr(III).
在遗传毒性研究中,同时进行了紫豌豆Klára的植物毒性和诱变性试验。对于植物毒性,生长抑制可按以下顺序排列:对于根:Ni(II) > Cr(VI) > Cr(III);芽部:Ni(II) > Cr(VI)≥Cr(III)。致突变性试验以苜蓿根尖为材料,至少在500个突变期检测到染色体畸变。不同浓度的金属对苜蓿染色体畸变率均有显著提高。Cr(VI)引起的像差最大,像差排列顺序为:Cr(VI) > Ni(II) > Cr(III)。通过对三棱草属植物花粉四分体微核频率的分析,确定了金属的遗传毒性效应。各试验金属对微核的刺激频率和基因毒性作用均无显著降低,其顺序为:Cr(VI)≥Ni(II) > Cr(III)。
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引用次数: 0
Use of phytoremediation techniques for elimination of lead from polluted soils 利用植物修复技术去除污染土壤中的铅
Q4 Agricultural and Biological Sciences Pub Date : 2021-11-22 DOI: 10.36547/nbc.1217
A. Hegedȕsová, Silvia Jakabová, Andrea Vargová, O. Hegedűs, T. Pernyeszi
The effect of chelating agent – EDTA (ethylene-diamine-tetra-acetic acid) was used for induced phytoextraction to increase intensity of lead transfer from roots to aboveground parts of garden pea. Pot experiments with contaminated soil substrata (50 mg Pb.kg-1 and 100 mg Pb.kg-1) were established for experimental purposes in growth chamber. The results showed that application of 5 and 10 mmol EDTA.kg-1 to experimental variants with 100 mg Pb.kg-1 doubled the increase of lead uptake by pea roots in comparison with variants without EDTA addition, which was statistically confirmed. Intensive lead transfer was observed from roots to aboveground parts of pea after application of 5 and 10 mmol EDTA.kg-1 in variant with 50 mg Pb.kg-1 (40-fold increase), as well as in variant with 100 mg Pb.kg-1 (17-fold increase). The results showed that induced phytoextraction can improve the mobility of lead from soil to plant roots. Application of 5 mmol EDTA.kg-1 resulted to 40-fold increase of lead transfer to green plant parts, despite the fact, that garden pea does not belong to conventional metal hyperaccumulating plant species. Following the results, pea could be used for decontamination of arable soil. The optimal EDTA concentration seems to be 5 mmol.kg-1. Therefore, application of 10 mmol EDTA.kg-1 decreased root mass about 55%, which resulted to decrease the intensity of lead uptake.
采用螯合剂EDTA(乙二胺-四乙酸)诱导植物提取,提高了豌豆根部向地上部分铅的迁移强度。污染土壤基质(50 mg Pb)盆栽试验。kg-1和100 mg pb (kg-1)在生长室中进行实验。结果表明,施用5和10 mmol EDTA后,kg-1到100 mg铅的实验变种。与未添加EDTA的变异相比,kg-1豌豆根系对铅的吸收增加了一倍,这在统计学上得到了证实。施用5和10 mmol EDTA后,豌豆根部向地上部的铅转移明显。kg-1的变体,含50mg铅。kg-1(增加40倍),以及100 mg Pb的变体。Kg-1(增加17倍)。结果表明,诱导植物提取能提高铅从土壤到植物根系的流动性。5 mmol EDTA的应用。Kg-1导致绿色植物部分铅转移增加40倍,尽管事实上,豌豆不属于传统的金属超积累植物物种。结果表明,豌豆可用于耕地土壤的净化。最佳的EDTA浓度似乎是5 mmol.kg-1。因此,应用10 mmol EDTA。Kg-1减少了约55%的根质量,导致铅的吸收强度降低。
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引用次数: 6
Microbial dechlorination of polychrolinated biphenyls 多氯联苯的微生物脱氯
Q4 Agricultural and Biological Sciences Pub Date : 2021-11-22 DOI: 10.36547/nbc.1158
V. Dudková, K. Demnerova, D. Bedard
Polychlorinated biphenyls (PCBs) are organic xenobiotics contaminating environment for at least 50 years. They could be eventually eliminated by various organisms under different conditions. The degree of chlorine substitution per biphenyl molecule influences biodegradability which decreases with increasing chlorination. Our work is focused on the PCBs biodegradation under anaerobic conditions. The suitable high chlorinated biphenyls are converted via reductive dechlorination to the chlorinated biphenyls with lower extent of chlorine, which could be eventually fully mineralized by aerobic bacteria. Microbial consortium was isolated from sediment of Strážský Creek (located near by plant producing PCBs in the past). This consortium was able to dechlorinate polychlorinated biphenyls under anoxic conditions. The effectiveness of this process was tested under different cultivation condition – different energetic sources (Aroclor 1248 or Aroclor 1260 or Delor 103 or Delor 106), addition of potential electron donors (pyruvate, lactate or acetate with hydrogen) and further if there is necessary to add yeast extract into fresh low sulphur cultivation media. Our microbial consortia so far do not need supplementation by non-contaminated sediment to maintain dechlorination activity. Addition of yeast extract is non essential, but needs to be further proved in serial transfers. In all cases (except acetate without yeast extract) dechlorination proceeds at meta- and flanked paraposition.
多氯联苯(PCBs)是污染环境至少50年的有机外生物质。它们最终会在不同的条件下被各种生物消灭。每个联苯分子的氯取代度影响生物降解性,随氯化程度的增加而降低。我们的工作主要集中在厌氧条件下多氯联苯的生物降解。合适的高氯联苯通过还原脱氯转化为低氯度的氯联苯,最终可被好氧菌完全矿化。从Strážský Creek(过去位于多氯联苯生产工厂附近)沉积物中分离出微生物联合体。该联合体能够在缺氧条件下脱氯多氯联苯。在不同的培养条件下测试了该过程的有效性-不同的能量源(Aroclor 1248或Aroclor 1260或Delor 103或Delor 106),添加潜在的电子供体(丙酮酸盐,乳酸盐或醋酸盐),如果有必要在新鲜的低硫培养培养基中添加酵母提取物。到目前为止,我们的微生物群落不需要补充未污染的沉积物来维持脱氯活性。酵母提取物的添加是不必要的,但需要在连续转移中进一步证明。在所有情况下(除了不含酵母提取物的醋酸盐)脱氯都是在中间和侧翼位置进行的。
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引用次数: 0
Antimicrobial effects of essential oils from Tanacetum vulgare L. and Salvia officinalis L., growing in Slovakia 产自斯洛伐克的丹参和鼠尾草精油的抗菌作用
Q4 Agricultural and Biological Sciences Pub Date : 2021-11-22 DOI: 10.36547/nbc.1273
M. Mikulášová, Š. Vaverková
Possible antimicrobial properties of essential oils isolated from Tanacetum vulgare L., and Salvia officinalis L., harvested from five different locations in Slovakia, were examined using the disc agar diffusion method and by the microdilution method. GC/MS analysis of the essential oil from Tanacetum vulgare L. resulted in the identification of 16 compounds constituting 82.1% of the total oil. Gram-positive bacteria, mainly Bacillus subtilis, were more susceptible to essential oils from both plants than were gramnegative species. Tested essential oils posses also anti-yeast activity. The shares of the constituents in the essential oils as well as their antimicrobial activity differed in dependence on the locality.
采用碟式琼脂扩散法和微量稀释法,研究了从斯洛伐克五个不同地点收获的Tanacetum vulgare L.和Salvia officinalis L.分离的精油可能的抗菌性能。GC/MS分析鉴定出16种化合物,占总挥发油的82.1%。革兰氏阳性菌,主要是枯草芽孢杆菌,比革兰氏阴性菌对两种植物的精油更敏感。经测试的精油还具有抗酵母菌活性。精油中各成分的含量及其抑菌活性因地而异。
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引用次数: 6
Chemometric characterization of textile waste waters from different processes 不同工艺纺织废水的化学计量学特征
Q4 Agricultural and Biological Sciences Pub Date : 2021-11-22 DOI: 10.36547/nbc.1272
T. Jerič, Darinka Brodnjak Vončina, Alenka Majcen Le Maréchal, Darja Kavšek
The aim of this work is focused on water quality classification of the textile waste water streams and evaluation of pollution. Data from the chemical characterization of the effluents were elaborated to identify a useful separation in potentially treatment for reuse. This was done with the aim of realizing a full scale characterization of effluents. In the two textile companies analyzed, machineries are used to carry out different production processes such as sizing and desizing, weaving, scouring, bleaching, mercerizing, carbonizing, fulling, dying and finishing. Different process effluents from the same machinery were found to be very diverse in pollution level. 25 and 49 samples of textile waste waters from two different textile companies were analysed and physical chemical measurements were performed. The following physicochemical and chemical water quality parameters were controlled: absorbance measured at three different wavelengths, pH, conductivity, turbidity, total suspended solids, volatile suspended solids, chemical oxygen demand, metals content (Ba, Ca, Cu, Mn, K, Sr, Fe, Al, Na) and total nitrogen content. For handling the results, basic statistical methods for the determination of mean and median values, standard deviations, minimal and maximal values of measured parameters and their mutual correlation coefficients, were performed. Different chemometric methods, namely, principal component analysis (PCA), cluster analysis (CA), and linear discriminant analysis (LDA) were used to find hidden information about textile waste water quality.
本文主要研究纺织废水流的水质分类和污染评价。阐述了废水化学特性的数据,以确定在可能的回用处理中有用的分离。这样做的目的是实现污水的全面表征。在分析的两家纺织公司中,机械被用来进行不同的生产过程,如上浆和退浆、织造、练、漂、丝光、碳化、填充、染色和整理。研究发现,同一台机器的不同工艺废水的污染水平差异很大。对来自两家不同纺织公司的25个和49个纺织废水样本进行了分析,并进行了物理化学测量。控制了三种不同波长下的吸光度、pH值、电导率、浊度、总悬浮物、挥发性悬浮物、化学需氧量、金属含量(Ba、Ca、Cu、Mn、K、Sr、Fe、Al、Na)和总氮含量。为了处理结果,采用了确定测量参数的平均值和中位数、标准差、最小值和最大值及其相互相关系数的基本统计方法。采用主成分分析(PCA)、聚类分析(CA)和线性判别分析(LDA)等不同的化学计量学方法发现纺织废水水质的隐藏信息。
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引用次数: 0
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Nova Biotechnologica et Chimica
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