M. Novosak, D. L. Winnik, Margarita Ester Laczeski, M. Quiroga
Streptococcus agalactiae, group B Streptococcus (GBS), infects and causes severe diseases in humans and numerous animal species, including fish, given its ability to cross the host-specific barrier. The emergence of antibiotic resistant GBS strains makes it necessary to look for alternatives to treat and prevent infections that it produces. The aim of the present study was to determine the antibacterial activity of ethanolic and aqueous extracts of medicinal plants from Misiones province, northeast Argentina, against GBS from humans and fish. We used human Streptococcus agalactiae ATCC® BAA-611™ and tilapia Streptococcus agalactiae ATCC® 51487™ strains. Minimum Inhibitory Dose (MID) was determined by the disc diffusion method. Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), MBC/MIC ratio, drug synergism with commercial antibiotics, and resistance assays were determined with extracts that showed antibacterial activity. Medium Lethal Dose 50 (LD50) was determined by the Artemia salina assay. For ethanolic and aqueous Eugenia uniflora L. extracts, we obtained a MID = 0.5 mg.disc-1. For both extracts of Eugenia uniflora L., the MIC and MBC values were 1 mg.mL-1 and 5 mg.mL-1, respectively. The MICI (MBC/MIC ratio) = 5 qualified the action of these extracts as bacteriostatic. The drug synergism assay with ampicillin, erythromycin, and clindamycin combination and extracts showed indifference. The LD50 of the aqueous extract was 0.82 mg.mL-1 indicating moderate toxicity. This work is a first step to identify chemical compounds in native medicinal plants of Misiones, Argentina, that could mean an alternative for the treatment of Streptococcus agalactiae infections.
{"title":"Antibacterial activity of medicinal plants against Streptococcus agalactiae","authors":"M. Novosak, D. L. Winnik, Margarita Ester Laczeski, M. Quiroga","doi":"10.36547/nbc.1264","DOIUrl":"https://doi.org/10.36547/nbc.1264","url":null,"abstract":"Streptococcus agalactiae, group B Streptococcus (GBS), infects and causes severe diseases in humans and numerous animal species, including fish, given its ability to cross the host-specific barrier. The emergence of antibiotic resistant GBS strains makes it necessary to look for alternatives to treat and prevent infections that it produces. The aim of the present study was to determine the antibacterial activity of ethanolic and aqueous extracts of medicinal plants from Misiones province, northeast Argentina, against GBS from humans and fish. We used human Streptococcus agalactiae ATCC® BAA-611™ and tilapia Streptococcus agalactiae ATCC® 51487™ strains. Minimum Inhibitory Dose (MID) was determined by the disc diffusion method. Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), MBC/MIC ratio, drug synergism with commercial antibiotics, and resistance assays were determined with extracts that showed antibacterial activity. Medium Lethal Dose 50 (LD50) was determined by the Artemia salina assay. For ethanolic and aqueous Eugenia uniflora L. extracts, we obtained a MID = 0.5 mg.disc-1. For both extracts of Eugenia uniflora L., the MIC and MBC values were 1 mg.mL-1 and 5 mg.mL-1, respectively. The MICI (MBC/MIC ratio) = 5 qualified the action of these extracts as bacteriostatic. The drug synergism assay with ampicillin, erythromycin, and clindamycin combination and extracts showed indifference. The LD50 of the aqueous extract was 0.82 mg.mL-1 indicating moderate toxicity. This work is a first step to identify chemical compounds in native medicinal plants of Misiones, Argentina, that could mean an alternative for the treatment of Streptococcus agalactiae infections.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49666688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Z. Alkay, Ezgi Metin Sagir, Muhammed Ozgolet, M. Durak
In this study, the technological properties of five different lactic acid bacteria (LAB) isolated from sourdough collected from three cities of Turkey (Gümüşhane, Manisa, Ankara) and Cyprus were investigated. For this purpose, antimicrobial, antifungal, phytase, and proteolytic activities of these bacteria and their effect on pH were examined. The pH of the prepared solutions decreased to 3.8 and 4.4 from 6.3 by LAB addition following 24 h incubation. Lactiplantibacillus plantarum O6F-25 strain showed the best inhibitory effect against four gram-positive and four gram-negative bacteria. In terms of proteolytic activity, Lactiplantibacillus plantarum 45MK-32 was the most effective strain. The antifungal effects of LAB were tested against Aspergillus flavus, Aspergillus niger, and Penicillium carneum. Levilactobacillus brevis KCO-48 was the most effective LAB strain. Phytase activities (710.40 – 840.37 U.mL-1) of LAB studied except Limosilactobacillus fermentum (29GT-19), which has the lowest phytase activity, were not significantly different (P < 0.05). This study revealed that sourdough LABs have the potential to be used as biopreservative and produce functional food products.
本研究研究了从土耳其三个城市(g姆哈内、马尼萨、安卡拉)和塞浦路斯的酵母中分离得到的五种不同乳酸菌的工艺特性。为此，研究了这些细菌的抗菌、抗真菌、植酸酶和蛋白水解活性及其对pH的影响。在24 h的孵育过程中，通过添加LAB，制备的溶液的pH值由6.3降低到3.8和4.4。植物乳杆菌O6F-25菌株对4种革兰氏阳性菌和4种革兰氏阴性菌的抑制效果最好。在蛋白水解活性方面，植物乳杆菌45MK-32是最有效的菌株。研究了乳酸菌对黄曲霉、黑曲霉和棕榈青霉的抑菌作用。短乳酸杆菌KCO-48是最有效的LAB菌株。除发酵limmosilactobacillus fermentum (29GT-19)植酸酶活性最低外，各组间植酸酶活性(710.40 ~ 840.37 u - ml -1)差异不显著(P < 0.05)。本研究表明，酵母实验室具有作为生物防腐剂和生产功能性食品的潜力。
{"title":"Characterization of functional effects of different LAB isolated from sourdoughs in Turkey","authors":"Z. Alkay, Ezgi Metin Sagir, Muhammed Ozgolet, M. Durak","doi":"10.36547/nbc.1155","DOIUrl":"https://doi.org/10.36547/nbc.1155","url":null,"abstract":"In this study, the technological properties of five different lactic acid bacteria (LAB) isolated from sourdough collected from three cities of Turkey (Gümüşhane, Manisa, Ankara) and Cyprus were investigated. For this purpose, antimicrobial, antifungal, phytase, and proteolytic activities of these bacteria and their effect on pH were examined. The pH of the prepared solutions decreased to 3.8 and 4.4 from 6.3 by LAB addition following 24 h incubation. Lactiplantibacillus plantarum O6F-25 strain showed the best inhibitory effect against four gram-positive and four gram-negative bacteria. In terms of proteolytic activity, Lactiplantibacillus plantarum 45MK-32 was the most effective strain. The antifungal effects of LAB were tested against Aspergillus flavus, Aspergillus niger, and Penicillium carneum. Levilactobacillus brevis KCO-48 was the most effective LAB strain. Phytase activities (710.40 – 840.37 U.mL-1) of LAB studied except Limosilactobacillus fermentum (29GT-19), which has the lowest phytase activity, were not significantly different (P < 0.05). This study revealed that sourdough LABs have the potential to be used as biopreservative and produce functional food products.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42884172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Mourad, Omrane Toumatia, S. Medjekal, B. Guessas, S. Cheriet, N. Bouras
A total of 33 lactic acid bacteria (LAB) were isolated from 5 samples of traditional dairy product (Jben) collected from various livestock farms in Oued El Malha, Djelfa, the Ouled Naïl Range of north-central Algeria. The taxonomic study of the strain B04 using a multiphase approach based on morphology, physiology, molecular (16S rRNA) and phylogenetic analyses allowed correlating this strain to Enterococcaceae. The difference of the 16S rRNA gene sequence of the isolate toward the most closely related genus Enterococcus was more than 6 % suggesting that the strain B04 represents a new genus. The results of the evaluation of some physiological tests indicated that B04 exhibited good biological activities including acidifying, proteolytic and bacterial inhibition. The strain B04 was antagonistic toward Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 25922, and Bacillus cereus ATCC 10876.
从阿尔及利亚中北部Oued El Malha、Djelfa和Oued Naïl山脉的各个畜牧场采集的5份传统乳制品样本中，共分离出33种乳酸菌（LAB）。B04菌株的分类学研究采用了基于形态学、生理学、分子（16S rRNA）和系统发育分析的多阶段方法，使该菌株与肠球菌科相关联。分离株的16S rRNA基因序列与亲缘关系最密切的肠球菌属的差异超过6%，表明菌株B04代表了一个新属。一些生理测试的评价结果表明，B04具有良好的生物活性，包括酸化、蛋白水解和细菌抑制。菌株B04对金黄色葡萄球菌ATCC 6538、大肠杆菌ATCC 25922和蜡样芽孢杆菌ATCC 10876具有拮抗作用。
{"title":"Phenotypic, genotypic, and technological characterization of new lactic acid bacterium isolated from traditional dairy product in Djelfa, Algeria","authors":"G. Mourad, Omrane Toumatia, S. Medjekal, B. Guessas, S. Cheriet, N. Bouras","doi":"10.36547/nbc.904","DOIUrl":"https://doi.org/10.36547/nbc.904","url":null,"abstract":"A total of 33 lactic acid bacteria (LAB) were isolated from 5 samples of traditional dairy product (Jben) collected from various livestock farms in Oued El Malha, Djelfa, the Ouled Naïl Range of north-central Algeria. The taxonomic study of the strain B04 using a multiphase approach based on morphology, physiology, molecular (16S rRNA) and phylogenetic analyses allowed correlating this strain to Enterococcaceae. The difference of the 16S rRNA gene sequence of the isolate toward the most closely related genus Enterococcus was more than 6 % suggesting that the strain B04 represents a new genus. The results of the evaluation of some physiological tests indicated that B04 exhibited good biological activities including acidifying, proteolytic and bacterial inhibition. The strain B04 was antagonistic toward Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 25922, and Bacillus cereus ATCC 10876.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49448724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present work aimed to study some pharmacological effects of extracts of the aerial parts of Polycarpon polycarpoides Fiori subsp. catalaunicum O. Bolòs & Vigo (Caryophyllaceae) widely distributed in the Mediterranean basin. The crude extracts were prepared using four solvents (petroleum ether, ethyl acetate, methanol, and methanol: water 1 : 1, v/v), and then examined for their phenolic contents using spectrophotometric methods and for their in vitro antioxidant activity and ability to inhibit urease, acetylcholinesterase, and α-amylase. Low levels of total phenolic were recorded ranging from 14.39 ± 3.40 to 101.84 μg.GAE.mg-1 of extract corresponding to the petroleum ether and ethyl acetate extracts, respectively. As for the flavonoids, very low values varying from 0.37 ± 0.13 to 4.22 ± 0.83 μg.GAE.mg-1 of extract were obtained. A moderate antioxidant effect was exerted by the extracts most often the methanol and ethyl acetate extracts were the most potent probably due to their polyphenolic content. Remarkable inhibitory effect has been exhibited by the extracts against α-amylase and more specifically, the petroleum ether extract displayed the strongest capacity with a percentage inhibition of 48.19 ± 2.99 % at 400 µg.mL-1. However, all extracts were inactive against urease and acetylcholinesterase. These results could constitute a starting point for carrying out more studies on the plant in order to assess the possibility of valuing it as a source of bioactive compounds.
{"title":"Phenolic contents, antioxidant, and enzyme inhibitory effects of crude extracts of Polycarpon polycarpoides Fiori subsp. catalaunicum O. Bolòs & Vigo","authors":"Madiha Arioua, Asma Cheribet, Lynda Gali","doi":"10.36547/nbc.1228","DOIUrl":"https://doi.org/10.36547/nbc.1228","url":null,"abstract":"The present work aimed to study some pharmacological effects of extracts of the aerial parts of Polycarpon polycarpoides Fiori subsp. catalaunicum O. Bolòs & Vigo (Caryophyllaceae) widely distributed in the Mediterranean basin. The crude extracts were prepared using four solvents (petroleum ether, ethyl acetate, methanol, and methanol: water 1 : 1, v/v), and then examined for their phenolic contents using spectrophotometric methods and for their in vitro antioxidant activity and ability to inhibit urease, acetylcholinesterase, and α-amylase. Low levels of total phenolic were recorded ranging from 14.39 ± 3.40 to 101.84 μg.GAE.mg-1 of extract corresponding to the petroleum ether and ethyl acetate extracts, respectively. As for the flavonoids, very low values varying from 0.37 ± 0.13 to 4.22 ± 0.83 μg.GAE.mg-1 of extract were obtained. A moderate antioxidant effect was exerted by the extracts most often the methanol and ethyl acetate extracts were the most potent probably due to their polyphenolic content. Remarkable inhibitory effect has been exhibited by the extracts against α-amylase and more specifically, the petroleum ether extract displayed the strongest capacity with a percentage inhibition of 48.19 ± 2.99 % at 400 µg.mL-1. However, all extracts were inactive against urease and acetylcholinesterase. These results could constitute a starting point for carrying out more studies on the plant in order to assess the possibility of valuing it as a source of bioactive compounds.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45265953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xylan is a complex hetero-polysaccharide consisting of different monosaccharides held together by glycosidic and ester bonds. Extracellular xylanase fashioned by numerous microbes principally from bacterial species such as Bacillus species are responsible for cleaving the glycosidic linkages. Microbial xylanases exhibit different substrate specificities and biochemical peculiarities. This study was carried out for optimization of cultivation conditions for xylanase production using the bacterium Lysinibacillus fusiformis and Kolanut husk as a component of cultivation medium. The bacterium was isolated from Kolanut plantation waste soil and screened for the production of xylanase qualitatively on xylan nutrient agar and quantitatively under submerged fermentation. The different conditions optimized included substrate concentration, additional sugars, incubation period, temperature, initial pH, nitrogen supplementation and inoculum mass through one factor at a time approach. Maximum xylanase production was obtained at substrate concentration of (1 % xylan and 1.5 % Kolanut husk), nitrogen source (yeast extract plus peptone), carbon source (sucrose), incubation period (24 h), pH (5.0), temperature (35 oC) and inoculum size (1 %). Lysinibacillus fusiformis has been proven to be a promising bacterium for xylanase production using Kolanut husk as substrate. The use of Kolanut husk as foremost carbon source is predominantly precious as being an agricultural waste, affordable, and locally available compared to expensive commercially sold xylan.
{"title":"Optimization of procedural factors for advanced xylanase synthesis by Lysinibacillus fusiformis using Kolanut husk as substrate","authors":"Suliat Olatidayo Omisore, D. Arotupin, M. Bayode","doi":"10.36547/nbc.1226","DOIUrl":"https://doi.org/10.36547/nbc.1226","url":null,"abstract":"Xylan is a complex hetero-polysaccharide consisting of different monosaccharides held together by glycosidic and ester bonds. Extracellular xylanase fashioned by numerous microbes principally from bacterial species such as Bacillus species are responsible for cleaving the glycosidic linkages. Microbial xylanases exhibit different substrate specificities and biochemical peculiarities. This study was carried out for optimization of cultivation conditions for xylanase production using the bacterium Lysinibacillus fusiformis and Kolanut husk as a component of cultivation medium. The bacterium was isolated from Kolanut plantation waste soil and screened for the production of xylanase qualitatively on xylan nutrient agar and quantitatively under submerged fermentation. The different conditions optimized included substrate concentration, additional sugars, incubation period, temperature, initial pH, nitrogen supplementation and inoculum mass through one factor at a time approach. Maximum xylanase production was obtained at substrate concentration of (1 % xylan and 1.5 % Kolanut husk), nitrogen source (yeast extract plus peptone), carbon source (sucrose), incubation period (24 h), pH (5.0), temperature (35 oC) and inoculum size (1 %). Lysinibacillus fusiformis has been proven to be a promising bacterium for xylanase production using Kolanut husk as substrate. The use of Kolanut husk as foremost carbon source is predominantly precious as being an agricultural waste, affordable, and locally available compared to expensive commercially sold xylan.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43900272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Different resistance mechanisms are involved in exhibiting resistance to different groups of antibiotics. Researchers are searching for new therapeutic options to encounter the emerging trend of microbial resistance. Bacteria were isolated from the extreme environment of Cholistan Desert and were screened for characterization. Potential metabolites that showed broad-spectrum activity were partially purified using silica gel chromatography and determined their minimum inhibitory concentration. A collection of 50 bacterial isolates from soil samples was screened for metabolite production and among them isolate R19 of Bacillus sp. and isolate A8 of Alcaligenes sp. had high similarity with strong antimicrobial metabolite producers. The growth of A8 was stable at slight acidic pH while R19 was best at neutral pH. Similarly, the best growth of A8 was observed at 37 °C while R19 at 35 °C. Minimum inhibitory concentration of purified compounds of Bacillus sp. were determined at concentration range of (3.12 – 100 %) against multidrug-resistant (MDR) strains of Shigella, Enterobacter aerogenes, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Klebsiella pneumonia and produced 10 – 25 mm zone of inhibition. Metabolites of Alcaligenes sp. were sufficient to inhibit the growth of all selected MDR bacteria at concentrations 12.25 – 100 % and shows 10 – 20 mm zone of inhibition. Bacillus sp. and Alcaligenes sp. can be used as producers of potential antibacterial metabolites. Proper utilization of selected metabolites can be helpful in combating emerging drug resistant pathogenic bacteria. In addition, further proteomic analysis and structural insight should be considered to elaborate their active ingredients and its efficacy.
{"title":"Elucidation of antibacterial activity of Bacillus sp. and Alcaligenes sp. metabolites against multidrug-resistant bacteria","authors":"Faheem Ullah, Sadir Zaman, Waheed Ullah, Shandana Ali, Muhammed Qasim, Niaz Muhammad, Momina Mehmood, Navid Ali, Niamat Khan","doi":"10.36547/nbc.1337","DOIUrl":"https://doi.org/10.36547/nbc.1337","url":null,"abstract":"Different resistance mechanisms are involved in exhibiting resistance to different groups of antibiotics. Researchers are searching for new therapeutic options to encounter the emerging trend of microbial resistance. Bacteria were isolated from the extreme environment of Cholistan Desert and were screened for characterization. Potential metabolites that showed broad-spectrum activity were partially purified using silica gel chromatography and determined their minimum inhibitory concentration. A collection of 50 bacterial isolates from soil samples was screened for metabolite production and among them isolate R19 of Bacillus sp. and isolate A8 of Alcaligenes sp. had high similarity with strong antimicrobial metabolite producers. The growth of A8 was stable at slight acidic pH while R19 was best at neutral pH. Similarly, the best growth of A8 was observed at 37 °C while R19 at 35 °C. Minimum inhibitory concentration of purified compounds of Bacillus sp. were determined at concentration range of (3.12 – 100 %) against multidrug-resistant (MDR) strains of Shigella, Enterobacter aerogenes, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Klebsiella pneumonia and produced 10 – 25 mm zone of inhibition. Metabolites of Alcaligenes sp. were sufficient to inhibit the growth of all selected MDR bacteria at concentrations 12.25 – 100 % and shows 10 – 20 mm zone of inhibition. Bacillus sp. and Alcaligenes sp. can be used as producers of potential antibacterial metabolites. Proper utilization of selected metabolites can be helpful in combating emerging drug resistant pathogenic bacteria. In addition, further proteomic analysis and structural insight should be considered to elaborate their active ingredients and its efficacy.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44085903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
W. Ansari, Asifa Irshad Kayani, Amjid Khan, A. Khan, Saman Shahid, Zainab Shahzadi, Hanan Mukhtar
Industrial biotechnology has a great emerging demand and sustainable expansion for mankind to utilize a variety of biodegradable material for the production of various alternative energy resources such as biogas and bioethanol. Researchers are interested in exploitation of novel fungal strains for the production of extracellular cellulase from the last few decades. This study was designed to assess the extracellular cellulase production potential of novel fungal strains of Sordaria fimicola first isolated from the evolution canyon, three located on South facing slope with xeric (S1, S2, S3) and other three on North facing slope with mesic (N5, N6, N7) environmental conditions. Based on initial and secondary screening two hyper producer strains from each slope were selected. The best activity for S2 was 3.125 U/ml and N6 exhibited 2.829 U/mL under optimized conditions of 14 d of incubation at 30 oC, pH 6.0, 1 mL inoculum and with 2% substrate (carboxy-methyl cellulose) concentration. Among the tested carbon and nitrogen sources, glucose proved to be best for both strains with S2 exhibiting maximum activity. Peptone and beef extract proved to be the best nitrogen sources for S2 and N6 respectively. The cellulase after characterization for temperature and pH showed slightly thermophilic nature. The cellulase was partial purification the highest cellulase activities as surviving in more xeric conditions which contribute more resistive and productive features in those microorganisms living in the harsh environment.
{"title":"Evaluation of extracellular cellulolytic potential of selected natural strains of a novel fungus Sordaria fimicola isolated from evolutionary canyon under submerged fermentation","authors":"W. Ansari, Asifa Irshad Kayani, Amjid Khan, A. Khan, Saman Shahid, Zainab Shahzadi, Hanan Mukhtar","doi":"10.36547/nbc.1319","DOIUrl":"https://doi.org/10.36547/nbc.1319","url":null,"abstract":"Industrial biotechnology has a great emerging demand and sustainable expansion for mankind to utilize a variety of biodegradable material for the production of various alternative energy resources such as biogas and bioethanol. Researchers are interested in exploitation of novel fungal strains for the production of extracellular cellulase from the last few decades. This study was designed to assess the extracellular cellulase production potential of novel fungal strains of Sordaria fimicola first isolated from the evolution canyon, three located on South facing slope with xeric (S1, S2, S3) and other three on North facing slope with mesic (N5, N6, N7) environmental conditions. Based on initial and secondary screening two hyper producer strains from each slope were selected. The best activity for S2 was 3.125 U/ml and N6 exhibited 2.829 U/mL under optimized conditions of 14 d of incubation at 30 oC, pH 6.0, 1 mL inoculum and with 2% substrate (carboxy-methyl cellulose) concentration. Among the tested carbon and nitrogen sources, glucose proved to be best for both strains with S2 exhibiting maximum activity. Peptone and beef extract proved to be the best nitrogen sources for S2 and N6 respectively. The cellulase after characterization for temperature and pH showed slightly thermophilic nature. The cellulase was partial purification the highest cellulase activities as surviving in more xeric conditions which contribute more resistive and productive features in those microorganisms living in the harsh environment.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43121434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Bellache, K. Moussaceb, J. Bollinger, F. Boudrahem
A local bentonite clay from Maghnia (Algeria) was activated with chemical method characterized and tested for its ability to adsorb Zinc (II) from refinery wastewater. Batch experiments were conducted to study the effects of the main parameters such as contact time, initial metal concentration and agitation speed on the adsorption of Zn (II) by local bentonite clay. Experiences have led to the following results: an adsorption rate of the order of 98 % with operating conditions of pH = 4.5, agitation speed of 400 rpm and temperature of 25 ºC. Pseudo-first-order, pseudo-second-order and intraparticle diffusion models were used to analyze the kinetic data obtained at different concentrations. The pseudo-second-order kinetic model agrees very well with the experimental results. In order to determine the best-fit isotherm, in the studied concentration range of Zn(II) at 25 ºC, the experimental equilibrium data were analyzed using tow adsorption isotherm models: Langmuir and Freundlich models, these two models give a good fit.
{"title":"Adsorption of Zn (II) ions from refinery wastewater by sulfuric acid-modified bentonite: Kinetic and isotherm studies","authors":"D. Bellache, K. Moussaceb, J. Bollinger, F. Boudrahem","doi":"10.36547/nbc.1267","DOIUrl":"https://doi.org/10.36547/nbc.1267","url":null,"abstract":"A local bentonite clay from Maghnia (Algeria) was activated with chemical method characterized and tested for its ability to adsorb Zinc (II) from refinery wastewater. Batch experiments were conducted to study the effects of the main parameters such as contact time, initial metal concentration and agitation speed on the adsorption of Zn (II) by local bentonite clay. Experiences have led to the following results: an adsorption rate of the order of 98 % with operating conditions of pH = 4.5, agitation speed of 400 rpm and temperature of 25 ºC. Pseudo-first-order, pseudo-second-order and intraparticle diffusion models were used to analyze the kinetic data obtained at different concentrations. The pseudo-second-order kinetic model agrees very well with the experimental results. In order to determine the best-fit isotherm, in the studied concentration range of Zn(II) at 25 ºC, the experimental equilibrium data were analyzed using tow adsorption isotherm models: Langmuir and Freundlich models, these two models give a good fit.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45744495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The lactic acid bacteria (LAB) isolated from human breast milk is known as probiotics and comprises numerous health benefits. This study aims to select and determine the species name of LAB based on the 16S rRNA gene, which has the potential to be the best indigenous probiotic. The method used included analysis of LAB resistance at acidic pH 2.0 and bile salts (0.5 %), antimicrobial activity against pathogenic microorganisms, and determining the autoaggregation properties. LAB isolates with the best ability in the analysis were then identified using a partial sequence of the 16S rRNA gene. The isolation and purification revealed eight LAB isolates with different parameters named as L19A, L19B, L19C, L19D, L19E, L19F, L19G, and L19H. Isolates L19A, L19E, and L19H have good tolerance ability against acid pH and bile salts, compared to others. Meanwhile, the L19H isolate had the strongest antimicrobial activity against pathogenic microorganisms E. coli ATCC 25922, S. aureus ATCC 25923, and C. albicans ATCC 11778, while the L19A had the highest hydrophobicity, autoaggregation, and coaggregation ability. Based on the partial sequence analysis of the 16S rRNA gene, the L19A, L19E, and L19H have similar values with L. casei, L. rhamnosus, and L. paracasei, respectively. These isolates belong to the L. casei group (LCG) from human breast milk, which can be used as an indigenic probiotic.
{"title":"Isolation and characterization of probiotic lactic acid bacteria from human breast milk","authors":"Nur Kusmiyati, Septian Tri Wicaksono, A. Sukarno","doi":"10.36547/nbc.1053","DOIUrl":"https://doi.org/10.36547/nbc.1053","url":null,"abstract":"The lactic acid bacteria (LAB) isolated from human breast milk is known as probiotics and comprises numerous health benefits. This study aims to select and determine the species name of LAB based on the 16S rRNA gene, which has the potential to be the best indigenous probiotic. The method used included analysis of LAB resistance at acidic pH 2.0 and bile salts (0.5 %), antimicrobial activity against pathogenic microorganisms, and determining the autoaggregation properties. LAB isolates with the best ability in the analysis were then identified using a partial sequence of the 16S rRNA gene. The isolation and purification revealed eight LAB isolates with different parameters named as L19A, L19B, L19C, L19D, L19E, L19F, L19G, and L19H. Isolates L19A, L19E, and L19H have good tolerance ability against acid pH and bile salts, compared to others. Meanwhile, the L19H isolate had the strongest antimicrobial activity against pathogenic microorganisms E. coli ATCC 25922, S. aureus ATCC 25923, and C. albicans ATCC 11778, while the L19A had the highest hydrophobicity, autoaggregation, and coaggregation ability. Based on the partial sequence analysis of the 16S rRNA gene, the L19A, L19E, and L19H have similar values with L. casei, L. rhamnosus, and L. paracasei, respectively. These isolates belong to the L. casei group (LCG) from human breast milk, which can be used as an indigenic probiotic.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48586758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. M. Cheban, A. Shcherbakov, N. Zholobak, M. Marchenko
The effect of various concentrations (1 μM – 100 mM) of citrate-stabilized cerium dioxide nanoparticles or cerium ions (3+) on the biomass production of two species of unicellular green algae Desmodesmus armatus (Chod.) Hegew and Acutodesmus dimorphus (Turpin) Tsarenko was studied, the amount of chlorophyll, proteins and lipids in the algae biomass was determined. It was shown that at the concentrations of 0.01 M to 0.1 M nanoparticles and cerium salt have pronounced toxic effects on the algal cultures, manifested by a sharp increase in the level of lipids in the biomass combined with the decrease in chlorophyll and protein. At lower concentrations, cerium dioxide nanoparticles stimulate algae biomass accumulation, probably due to a change in key metabolic pathways, accompanied by an increase in the accumulation of carbohydrates in the biomass. For cerium salt, these effects are less pronounced. Thus, depending on the concentration of the objects used, it is possible to obtain an increase in the food biomass production enriched with lipids or carbohydrates as appropriate to the biotechnological objectives.
{"title":"The specificity of changes in key performance indicators of green algae of the family Scenedesmaceae under the influence of cerium","authors":"L. M. Cheban, A. Shcherbakov, N. Zholobak, M. Marchenko","doi":"10.36547/nbc.954","DOIUrl":"https://doi.org/10.36547/nbc.954","url":null,"abstract":"The effect of various concentrations (1 μM – 100 mM) of citrate-stabilized cerium dioxide nanoparticles or cerium ions (3+) on the biomass production of two species of unicellular green algae Desmodesmus armatus (Chod.) Hegew and Acutodesmus dimorphus (Turpin) Tsarenko was studied, the amount of chlorophyll, proteins and lipids in the algae biomass was determined. It was shown that at the concentrations of 0.01 M to 0.1 M nanoparticles and cerium salt have pronounced toxic effects on the algal cultures, manifested by a sharp increase in the level of lipids in the biomass combined with the decrease in chlorophyll and protein. At lower concentrations, cerium dioxide nanoparticles stimulate algae biomass accumulation, probably due to a change in key metabolic pathways, accompanied by an increase in the accumulation of carbohydrates in the biomass. For cerium salt, these effects are less pronounced. Thus, depending on the concentration of the objects used, it is possible to obtain an increase in the food biomass production enriched with lipids or carbohydrates as appropriate to the biotechnological objectives.","PeriodicalId":19210,"journal":{"name":"Nova Biotechnologica et Chimica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46528781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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