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Analgesic, muscle relaxant, and anti-inflammatory potential of of flavonoids isolated from Euphorbia pulcherrima: Insights from molecular docking ligand retrieval, protein retrieval, and visualization and analysis investigation 大戟黄酮类化合物的镇痛、肌肉松弛和抗炎潜能:来自分子对接配体检索、蛋白质检索、可视化和分析研究的见解
IF 3.3 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-03-01 Epub Date: 2026-01-16 DOI: 10.1016/j.nfs.2026.100261
Abdur Rauf , Naveed Muhammad , Muhammad Umer Khan , Maha Munir , Yasir Anwar , Bassam Oudh Aljohny , Kamala Badalova , Saima Naz , Zuneera Akram

Background

Euphorbia pulcherrima Willd Ex Koltz is traditionally used for toothache, fracture, traumatic haemorrhage, etc. Nevertheless, although it is used, the most obvious gap is a knowledge of the mechanism underlying the described effects and bioactive compounds. The objective of this study was to prepare flavonoids in the methanolic bark extract of E. pulcherrima and determine their analgesic, anti-inflammatory, and muscle-relaxant properties, and to discuss their binding affinity to COX-2, TRPV1, and GABA-A receptors using molecular docking. Compound 1 (5,7,8,3′,4′-pentahydroxy-3-methoxyflavone) was more effective as an analgesic, with a significant (p < 0.001) increase in latency time (22.70–24.12 s) over compound 2 (kaempferol-3-B-D-glucopyranoside). The analgesic action of compound 1 implies the involvement with the central pain receptors similar to the opioid and GABAergic systems. Both compounds demonstrated dose-effective muscle relaxant activities, with compound 1 demonstrating 70% inhibition in the inclined plane model. The effects on anti-inflammatory effects were also notable with compound 1 reducing paw edema by 57.54% in the initial phase and 80% inhibition in the late phase, whereas compound 2 showed an inhibition rate of 87% in the third hour after induction. The docking experiments established that the two compounds displayed high binding affinities with COX-2, TRPV1, and GABA-A receptors and confirmed their potential as multi-target agents. The findings highlight the value of E. pulcherrima-derived flavonoids as multi-target therapeutics against pain, inflammation, and muscle-related disorders, which can be used in the development of new plant-based drugs with reduced side effects and improved effects.
传统上用于牙痛、骨折、外伤性出血等。然而,尽管它被使用了,但最明显的差距是对所描述的作用和生物活性化合物的机制的了解。本研究的目的是通过分子对接的方法,从白参树皮甲醇提取物中制备黄酮类化合物,并测定其镇痛、抗炎和肌肉松弛的特性,探讨其与COX-2、TRPV1和GABA-A受体的结合亲和力。化合物1(5,7,8,3 ',4 ' -五羟基-3-甲氧基黄酮)作为镇痛药更有效,潜伏期(22.70-24.12 s)比化合物2(山奈酚-3- b - d -葡萄糖吡喃苷)显著(p < 0.001)增加。化合物1的镇痛作用与中枢疼痛受体有关,类似于阿片和gaba能系统。两种化合物均显示出剂量有效的肌肉松弛活性,其中化合物1在斜面模型中显示出70%的抑制作用。抗炎作用也很明显,化合物1在诱导后第3 h对足跖水肿的抑制率为87%,而化合物1在诱导后第3 h对足跖水肿的抑制率为57.54%,后期抑制率为80%。对接实验证实,这两种化合物与COX-2、TRPV1和GABA-A受体具有较高的结合亲和力,证实了它们作为多靶点药物的潜力。这些发现突出了白参黄酮作为治疗疼痛、炎症和肌肉相关疾病的多靶点药物的价值,可用于开发副作用更小、疗效更好的新型植物性药物。
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引用次数: 0
Preparation of Korean Kimchi-seasoned laver (Neopyropia yezoensis) and Nutritional characteristics with Shelf-life evaluation through physicochemical, sensory and microbiological methods 用理化、感官和微生物学方法对泡菜紫菜的制备、营养特性和保质期进行评价
IF 3.3 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-03-01 Epub Date: 2025-12-10 DOI: 10.1016/j.nfs.2025.100254
Ramakrishna Chilakala , Kang Ho Ko , Min Seouk Jung , Jong Won Han , Eun-Sik Kim , Sun Hee Cheong
Seaweed is a nutritious and healthy functional food, while preparing seaweed food poses a number of difficulties due to its highly perishable and their limited processing techniques. This study aimed to develop kimchi seasoned laver (KSL) to meet the global demand for innovative seasoned laver products. In this preparation method, different proportions of kimchi (6.75–15.75 %), kimchi stew (6.75–15.75 %) are used, along with 4.5 % red chili, 28.4 % sunflower oil, and 44.6 % seaweed. To ascertain the food quality and shelf-life of roasted control laver (CON) and KSL samples during storage for seven weeks, the physicochemical, sensory and microbiological attributes were analyzed periodically. The results showed that moisture (1.1–1.5 %), ash (7–8 %), crude fat (40.1–45.1 %) contents were significantly not different between CON and KSL samples. However, crude protein levels decreased significantly from 22 % in CON to 16.5–18 % in KSL samples. Total polyunsaturated fatty acids (PUFAs) increased significantly from 11.5 % (CON) to 48.2–48.4 % (KSL), and the PUFA/SFA ratios improved from 1.21 to 4.05. In KSL samples. The amount of free glutamic acid increased from 17.4 % (CON) to 28.9–34.4 %, which improved the umami flavor. During storage, peroxide levels increased from 10.3 to 14.61 mEq/kg in CON samples and from 16.14–19.76 to 32.57–34.1 mEq/kg in KSL samples. Acid values remained below 1.0 mg/g and the TBARS levels ranged from 1.25 to 1.59 μg MDA/g, all of which were within acceptable ranges. Moreover, E-coli was not detected, and aerobic plate counts for both CON and KSL laver samples remained within acceptable range (4.72–5.41 log CFU/g after 7 weeks). Sensory evaluation revealed that KSL samples displayed comparatively better sensory attributes (6–7 weeks) than CON laver (3–4 weeks), with KSL-2 (11.25 % kimchi +11.25 % kimchi stew) shows highest overall acceptance. The results indicated that kimchi-seasoned laver with optimal formulation demonstrated improved oxidative stability, enhanced nutritional properties, and extended shelf-life, supporting quality control in the food sector and enabling the industrialization of the KSL laver product market.
海藻是一种营养健康的功能性食品,但由于其极易腐烂和加工技术有限,制备海藻食品面临许多困难。本研究旨在开发泡菜调味紫菜(KSL),以满足全球对创新调味紫菜产品的需求。用不同比例的泡菜(6.75 ~ 15.75%)、泡菜炖菜(6.75 ~ 15.75%)、4.5%的红辣椒、28.4%的葵花籽油、44.6%的海藻制作。为确定烤制对照紫菜(CON)和KSL样品在贮藏7周内的食品质量和保质期,对其理化、感官和微生物特性进行了定期分析。结果表明:CON与KSL样品的水分(1.1 ~ 1.5%)、灰分(7 ~ 8%)、粗脂肪(40.1 ~ 45.1%)含量差异不显著;然而,粗蛋白质水平从CON的22%显著下降到KSL样品的16.5 - 18%。总多不饱和脂肪酸(PUFAs)从11.5% (CON)显著增加到48.2 ~ 48.4% (KSL), PUFA/SFA比值从1.21提高到4.05。在KSL样品中。游离谷氨酸含量由17.4% (CON)提高到28.9 ~ 34.4%,改善了鲜味。在储存过程中,CON样品的过氧化物水平从10.3 mEq/kg增加到14.61 mEq/kg, KSL样品的过氧化物水平从16.14-19.76增加到32.57-34.1 mEq/kg。酸值保持在1.0 mg/g以下,TBARS水平在1.25 ~ 1.59 μg MDA/g之间,均在可接受范围内。此外,没有检测到大肠杆菌,CON和KSL紫菜样品的好氧平板计数在7周后保持在可接受范围内(4.72-5.41 log CFU/g)。感官评价显示,KSL样品的感官属性(6-7周)比CON紫菜(3-4周)要好,其中KSL-2(11.25%泡菜+ 11.25%泡菜炖菜)的总体接受度最高。结果表明,优化配方后的泡菜酱菜氧化稳定性提高,营养特性增强,保质期延长,为食品行业的质量控制提供了支持,有利于泡菜酱菜产品市场的产业化。
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引用次数: 0
Effect of composite blend of mushroom (Pleurotus ostreatus) and pumpkin seed (Cucurbita moschata) powder fortification on the physicochemical, textural, and sensory properties of wheat bread 香菇(Pleurotus ostreatus)和南瓜子(Cucurbita moschata)粉复合强化对小麦面包理化、质地和感官特性的影响
IF 3.3 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-03-01 Epub Date: 2026-02-08 DOI: 10.1016/j.nfs.2026.100264
Sushmita Kabir , Md. Abdullah Al Noman , Anannya Barua Nijhum , Iqbal Hossain , Md. Asadujjaman Robin , Saydur Rahman , Juwel Rana , Afzal Rahman , Poly Karmoker , Md. Anisur Rahman Mazumder
This study investigated the effects of composite blend of oyster mushroom (Pleurotus ostreatus) and pumpkin seed (Cucurbita moschata) powder on the physicochemical, textural, and sensory properties of whole-wheat bread. Whole-wheat flour was substituted with the composite blend consisting of a 1:1 ratio (w/w) at three levels: 5% (S2), 10% (S3), and 15% (S4) total replacement, and compared against a 100% whole-wheat control (S1). This significantly (p ≤ 0.05) enhanced the nutritional profile of the bread, with protein content increasing from 9.46% in the control to 13.43% in S4, and dietary fiber increasing from 0.51% to 1.40%. However, fortification introduced a dose-dependent trade-off, leading to a denser loaf with reduced specific volume and increased hardness, which rose from 55.02 N in S1 to 99.88 N in S4. Sensory analysis revealed that the 10% substitution level (S3) achieved the highest scores for flavor and overall acceptability, surpassing the control bread. This formulation was identified as the most suitable, successfully balancing substantial nutritional improvements with superior consumer preference. These findings demonstrate that a 10% (S3) substitution is a recommended level for developing a commercially viable, nutrient-enriched bread that meets both health interests and sensory standards.
本研究考察了平菇和南瓜籽粉复合配料对全麦面包理化、质构和感官特性的影响。在5% (S2)、10% (S3)和15% (S4)三个水平上,用1:1比例(w/w)的复合混合物替代全麦面粉,并与100%全麦对照(S1)进行比较。这显著(p≤0.05)提高了面包的营养成分,蛋白质含量从对照组的9.46%提高到S4的13.43%,膳食纤维从0.51%提高到1.40%。然而,强化带来了剂量依赖性的权衡,导致面包更致密,比体积减小,硬度增加,从S1的55.02 N上升到S4的99.88 N。感官分析显示,10%替代水平(S3)的面包在风味和总体可接受性方面得分最高,超过了对照面包。这种配方被认为是最合适的,成功地平衡了大量的营养改善和优越的消费者偏好。这些发现表明,10% (S3)的替代是开发商业上可行的、营养丰富的面包的推荐水平,既符合健康利益,又符合感官标准。
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引用次数: 0
Effect of the addition of water extract from guelder rose (Viburnum opulus) flowers on physicochemical and sensory properties of veal burgers 枸杞花水提物的加入对牛肉汉堡理化及感官特性的影响
IF 3.3 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-03-01 Epub Date: 2026-02-11 DOI: 10.1016/j.nfs.2026.100265
Marek Kowalczyk , Piotr Domaradzki , Mariusz Florek , Małgorzata Materska , Paweł Żółkiewski , Piotr Skałecki , Anna Teter , Agnieszka Kaliniak-Dziura , Monika Pecio , Małgorzata Dmoch , Ewa Poleszak
The use of plant extracts is gaining increasing popularity among both food producers and consumers. Extracts can impart health-promoting properties and improve the antioxidant properties of food products, while stabilising sensitive compounds can positively extend the shelf life of food. The objective of the study was to assess the effect of the addition of water extract from guelder rose flowers (GRE) at concentrations of 0.05%, 0.1% and 0.4% on the physicochemical properties (pH, water activity, texture, colour, lipid oxidation - TBARS, α-tocopherol content), sensory properties (beef aroma, off-odours, juiciness, tenderness, beef flavour, off-flavours) and overall quality of veal burgers stored refrigerated for 15 days. Burgers containing 0.1% and 0.4% GRE showed a significantly lower pH (on average, 5.56 in both GRE treatments vs. 5.61 in control samples) and higher purge loss after 10 days (2.39% and 2.90%, respectively, vs. 1.00% in control samples) and 15 days of storage (3.04% and 3.80%, respectively, vs. 1.22% in control samples; P < 0.05). The addition of the extract, irrespective of its concentration, slowed down lipid oxidation and significantly increased α-tocopherol retention (2.15–2.21 μg/g in GRE treatments vs. 1.83 μg/g in control samples; P < 0.05). However, at concentrations of 0.1% and 0.4%, it worsened the sensory properties while increasing the intensity of off-odours and off-tastes, thus lowering the overall quality rating (on average, 6.75 and 5.75, respectively, vs. 7.37 in control samples; P < 0.05). Among the tested GRE levels, a concentration of 0.05% was associated with the most favourable overall outcomes, without significant negative effects on water-holding capacity or sensory characteristics (P > 0.05), while improving α-tocopherol retention and reducing TBARS values. The use of water extract from guelder rose flowers at an appropriate concentration can improve the shelf life and oxidative stability of burgers without adversely affecting their water-holding capacity and sensory quality.
植物提取物的使用越来越受到食品生产商和消费者的欢迎。提取物具有促进健康和提高食品抗氧化性能的作用,而稳定敏感化合物可以积极延长食品的保质期。本研究的目的是评估添加0.05%、0.1%和0.4%浓度的白骨精玫瑰水提取物(GRE)对冷藏15天的小牛肉汉堡的理化性质(pH值、水活度、质地、颜色、脂质氧化- TBARS、α-生育酚含量)、感官性质(牛肉香气、异味、多汁性、嫩度、牛肉风味、异味)和整体质量的影响。含有0.1%和0.4% GRE的汉堡在10天(分别为2.39%和2.90%,对照样品为1.00%)和15天(分别为3.04%和3.80%,对照样品为1.22%,P < 0.05)后,pH值显著降低(平均为5.56,对照样品为5.61),吹扫损失较高。无论提取物浓度如何,其添加均能减缓脂质氧化,显著提高α-生育酚保留率(GRE组为2.15 ~ 2.21 μg/g,对照组为1.83 μg/g, P < 0.05)。然而,在0.1%和0.4%的浓度下,它恶化了感官性能,同时增加了异味和异味的强度,从而降低了整体质量等级(平均分别为6.75和5.75,而对照样品为7.37;P < 0.05)。在测试的GRE水平中,0.05%的浓度与最有利的总体结果相关,对持水能力或感官特征没有显著的负面影响(P > 0.05),同时改善α-生育酚潴留并降低TBARS值。在不影响汉堡保水能力和感官品质的前提下,适当浓度的金玫瑰水提物可提高汉堡的保存期和氧化稳定性。
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引用次数: 0
Atmospheric cold plasma-assisted extraction of basil (Ocimum basilicum) leaves bioactives for yogurt fortification: A food engineering approach 常压冷等离子体辅助提取罗勒叶的生物活性,用于酸奶强化:食品工程方法
IF 3.3 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-03-01 Epub Date: 2026-03-03 DOI: 10.1016/j.nfs.2026.100266
Basheer M. Iqdiam, Ali Nazar Abdul-Ghaffar, Entisar Dawood Mustafa
The study evaluated the effectiveness of dielectric barrier atmospheric cold plasma (DBD-ACP) as a sustainable, non-thermal pretreatment to enhance the extraction of bioactive compounds from basil leaves and improve the quality of fortified yogurt during refrigerated storage. A 10 g sample of basil powder was exposed to DBD-ACP for 60s, 90s, and 120 s. The plasma aqueous extraction efficiency was compared with ethanol extraction. The best plasma-treated extract was incorporated into yogurt, and enriched yogurt was assessed for microbiological, chemical, and sensory properties. Results illustrated that DBD-ACP pretreatment significantly increased total phenolic content (25.57 mg GAE/g), total flavonoid content (14.33 mg QE/g), and antioxidant activity (78.4% DPPH) after 120 s of exposure, along with elevated levels of eugenol and linalool. Engineering analysis demonstrated high efficiency, with specific energy input (900–1800 J/g), extraction yield (6.12–24.46%), system performance coefficient (0.022–0.037 g/J), and energy efficiency (0.0068–0.0136 mg/J) increasing with treatment time. Incorporating plasma-treated basil extracts (TY) into yogurt significantly reduced total viable counts (2.53–3.92 log CFU/g) and yeast/mold counts (1.09–3.12 log CFU/g), while maintaining a higher pH (4.48 ± 0.02) and lower titratable acidity (0.84 ± 0.02%). Sensory evaluations confirmed enhanced flavor, color, and overall acceptability, particularly in TY5 samples. Overall, DBD-ACP proved to be an energy-efficient, solvent-free technology that enhanced bioactive recovery and produced stability, offering a promising green approach for developing functional, clean-label dairy products.
本研究评估了介质阻挡大气冷等离子体(DBD-ACP)作为一种可持续的非热预处理的有效性,以提高罗勒叶中生物活性化合物的提取,并提高强化酸奶在冷藏期间的质量。将10 g罗勒粉样品分别暴露于DBD-ACP中60、90和120 s。比较了血浆水萃取和乙醇萃取的效率。将最好的血浆处理提取物掺入酸奶中,并对浓缩酸奶的微生物学、化学和感官特性进行评估。结果表明,经DBD-ACP预处理,120s后总酚含量(25.57 mg GAE/g)、总黄酮含量(14.33 mg QE/g)和抗氧化活性(78.4% DPPH)显著升高,丁香酚和芳樟醇含量显著升高。工程分析表明,随着处理时间的延长,系统比能输入(900 ~ 1800 J/g)、萃取率(6.12 ~ 24.46%)、系统性能系数(0.022 ~ 0.037 g/J)、系统能效(0.0068 ~ 0.0136 mg/J)均呈递增趋势。将经血浆处理的罗勒提取物(TY)加入酸奶中,可显著降低总活菌数(2.53-3.92 log CFU/g)和酵母/霉菌数(1.09-3.12 log CFU/g),同时保持较高的pH值(4.48±0.02)和较低的可滴定酸度(0.84±0.02%)。感官评估证实了风味、颜色和整体可接受性的增强,特别是TY5样品。总的来说,DBD-ACP被证明是一种节能、无溶剂的技术,提高了生物活性回收率和生产稳定性,为开发功能性、清洁标签的乳制品提供了一种有前途的绿色方法。
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引用次数: 0
Anti-adhesion bioactivity of soybean tempeh against enterotoxigenic Escherichia coli F4 through supplementation with exopolysaccharide-producing Leuconostoc mesenteroides 大豆豆豉对产肠毒素大肠杆菌F4的抗黏附生物活性研究
IF 3.3 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-03-01 Epub Date: 2026-01-19 DOI: 10.1016/j.nfs.2026.100262
Theodorus Eko Pramudito , Cynthia E. Klostermann , Dennis T.M. van den Berg , Eddy J. Smid , Henk A. Schols
Soybean tempeh can reduce the adhesion of diarrheagenic enterotoxigenic Escherichia coli (ETEC) to the intestinal surface due to the presence of carbohydrates that can bind to the pathogen's fimbrial adhesin. Exopolysaccharides (EPS) produced by lactic acid bacteria (LAB) such as Leuconostoc mesenteroides also exhibit anti-adhesion bioactivity against ETEC. Tempeh supplemented with EPS-producing L. mesenteroides strains (LmWA and LmWN) to improve its anti-adhesion bioactivity was produced. Both strains persisted in the fermentation process and produced dextran (α-1,6-linked glucan) in the supplemented tempeh. LmWN supplementation resulted in a higher amount of dextran in tempeh (0.44% w/w) compared to LmWA (0.22% w/w). The dextrans obtained from both LAB supplementations consisted of two molecular weight (Mw) populations of ∼750 to >1000 kDa and ∼ 5 kDa. Although both strains are known to also produce levan (β-2,6-linked fructan), such EPS was not detected in LAB-supplemented tempeh. LAB-supplemented tempeh reduced adhesion of pig-associated ETEC F4 to porcine mucin by three to four times compared to non-supplemented tempeh. The role of dextran in such bioactivity was confirmed by increased adhesion upon treatment of the tempeh extract with dextranase. In conclusion, the production and presence of bioactive dextran with anti-adhesion bioactivity against ETEC in tempeh made with the supplementation of EPS-producing LAB strain to soybean fermentation has been demonstrated. This study demonstrates that co-fermentation with EPS-producing LAB is a viable strategy to develop food products with an anti-adhesion bioactivity against ETEC.
大豆豆豉可以减少致泻性肠毒素大肠杆菌(ETEC)对肠道表面的粘附,因为其存在的碳水化合物可以与病原体的菌毛粘附素结合。乳酸菌(LAB)产生的胞外多糖(EPS)对ETEC也具有抗黏附的生物活性。在豆豉中添加产eps的肠系膜乳杆菌(LmWA和LmWN)以提高其抗黏附活性。这两种菌株在发酵过程中都持续存在,并在添加豆豉后产生葡聚糖(α-1,6-链葡聚糖)。添加LmWN可使豆豉中葡聚糖含量(0.44% w/w)高于添加LmWA (0.22% w/w)。从两种LAB添加物中获得的右旋糖酐由两个分子量(Mw)群体组成,分别为~ 750至>;1000 kDa和~ 5 kDa。虽然已知这两种菌株也产生利凡(β-2,6-链果聚糖),但在实验室补充的豆豉中未检测到这种EPS。与未添加的豆豉相比,实验室添加的豆豉减少了猪相关ETEC F4对猪粘蛋白的粘附,减少了三到四倍。葡聚糖在这种生物活性中的作用被葡聚糖酶处理后豆豉提取物的粘附性增加所证实。综上所述,在大豆发酵过程中添加产eps的LAB菌株,在豆豉中产生并存在具有抗ETEC生物活性的葡聚糖。该研究表明,与产eps的乳酸菌共发酵是开发具有抗粘附生物活性的ETEC食品的可行策略。
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引用次数: 0
Nutritional and health-promoting quality of organic milk of Saanen goats and Jersey cows and yoghurt produced from it 萨南山羊和泽西奶牛有机奶的营养和健康品质及其生产的酸奶
IF 3.3 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-03-01 Epub Date: 2026-01-27 DOI: 10.1016/j.nfs.2026.100263
Aldona Kawęcka, Marta Pasternak
The aim of the study was to assess the nutritional and health-promoting value of goat and cow milk and yoghurt from organic production, with particular emphasis on their antioxidant properties. The research material consisted of milk obtained from Saanen goats and Jersey cows and yoghurt produced from it. The study showed that the products differed in composition depending on the species and month of raw material acquisition and production. The milk of Jersey cows was characterised by a higher content of dry matter, protein and fat and was of higher caloric value than the milk of Saanen goats, while the content of minerals was higher in goat milk. The protein fraction in goat and cow milk was similar, but the differences in proportions may result in different functional properties. Yoghurts made from cow milk contained more dry matter, protein and ash, while yoghurts made from goat milk were characterised by a more favourable fatty acid profile. The phenolic compounds content were higher in yoghurts than in milk of both species, and the antioxidant potential of yoghurts was higher in cow products. The obtained results indicate that the products can be consumed by various groups of consumers with different health and nutritional needs.
这项研究的目的是评估有机生产的羊奶、牛奶和酸奶的营养和健康促进价值,特别强调它们的抗氧化特性。研究材料包括从萨宁山羊和泽西奶牛获得的牛奶以及从中生产的酸奶。研究表明,不同原料获取和生产的品种和月份不同,产品的成分也不同。泽西奶牛乳的干物质、蛋白质和脂肪含量高于萨宁山羊乳,热值也高于萨宁山羊乳,矿物质含量高于萨宁山羊乳。羊奶和牛奶中的蛋白质含量相似,但比例的差异可能导致其功能特性的不同。由牛奶制成的酸奶含有更多的干物质、蛋白质和灰分,而由羊奶制成的酸奶具有更有利的脂肪酸特征。酸奶中的酚类化合物含量高于两种牛奶,酸奶的抗氧化潜力在奶牛产品中更高。所得结果表明,该产品适合不同健康和营养需求的消费者群体。
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引用次数: 0
Safety evaluation of a soluble granular food containing quercetin glycosides for healthy adults: A randomized controlled trial 含槲皮素糖苷的可溶性颗粒食品对健康成人的安全性评价:一项随机对照试验
IF 3.3 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-03-01 Epub Date: 2025-12-23 DOI: 10.1016/j.nfs.2025.100258
Mui Iida , Misaki Onozato , Hideyuki Sasaki , Izumi Yamasaki , Takao Tanaka , Hidenori Obata , Takayuki Izumo , Yoshihiro Nakao , Ikuo Fukuhara

Background

Quercetin glycosides (QG), enzymatically modified isoquercitrin derivatives, are widely used in functional foods for their antioxidant, antihypertensive, and lipid metabolism benefits. Previous safety studies have confirmed tolerability at intake levels up to 480 mg/day for four weeks. However, Japanese regulatory guidelines require overdose safety evaluations at approximately five times the effective dose (110 mg/day), and data on higher intake levels remain limited.

Objective

To evaluate the safety of daily consumption of a food product containing 550 mg/day of QG approximately five times the effective dose for four weeks in healthy adults.

Methods

A randomized, double-blind, placebo-controlled, parallel-group trial was conducted at a single clinical site in Japan. Healthy adults aged 20–64 years with BMI 18.5–29.9 kg/m2 were enrolled and randomized (1:1) to receive either soluble granular food containing QG (550 mg/day) or placebo for four weeks. Randomization was stratified by sex, age, and BMI using blocked allocation. Both participants and investigators were blinded to group assignments. The primary endpoint was the incidence of adverse reactions. Secondary endpoints included the incidence of adverse events and changes in physiological and clinical parameters. Safety assessments were performed at baseline, week 2, and week 4.

Results

Of 149 screened individuals, 48 were randomized and 47 completed the study(QG group: n = 23, control group: n = 24). No adverse reactions attributable to the test food occurred. Ten adverse events were reported (QG: 4 events in 4 participants, placebo: 5 events in 4 participants), all mild and unrelated to the intervention. In addition, no abnormal changes were observed in clinical test values. In the QG group, minor intragroup changes in biochemical parameters were noted, but all values remained within standard ranges. Also, no significant intergroup differences were detected.

Conclusion

Daily intake of 550 mg/day QG for four weeks was safe in healthy adults, with no adverse reactions or clinically meaningful changes in safety parameters. These findings support the safety of QG at doses exceeding previously tested levels. Further research is needed to assess long-term safety and higher dosages. Powered by Editorial Manager® and ProduXion Manager® from Aries Systems Corporation.
Trial registration: The clinical trial was registration with the University Hospital Medical Information Network Clinical Trials Registry (UMIN-CTR, UMIN ID: UMIN000053632); https://center6.umin.ac.jp/cgi-open-bin/ctr_e/ctr_view.cgi?recptno=R000061206.
槲皮素糖苷(QG)是酶修饰的异槲皮素衍生物,因其抗氧化、抗高血压和脂质代谢的益处而被广泛应用于功能性食品中。先前的安全性研究已经证实,在摄入480毫克/天的水平下,持续四周的耐受性。然而,日本的监管指南要求在大约5倍于有效剂量(110毫克/天)的剂量下进行过量安全性评估,关于更高摄入量的数据仍然有限。目的评价健康成人连续4周每日食用含550mg /天QG(约为有效剂量的5倍)的食品的安全性。方法在日本进行一项随机、双盲、安慰剂对照、平行组试验。年龄在20-64岁、体重指数为18.5-29.9 kg/m2的健康成年人被招募并随机(1:1)接受含有QG (550 mg/天)的可溶性颗粒食物或安慰剂,为期四周。随机化按性别、年龄和BMI采用阻塞分配进行分层。参与者和调查人员都对小组分配不知情。主要终点是不良反应的发生率。次要终点包括不良事件的发生率以及生理和临床参数的变化。在基线、第2周和第4周进行安全性评估。结果149例筛选个体中,48例随机入选,47例完成研究(QG组:n = 23,对照组:n = 24)。未发生与试验食品有关的不良反应。报告了10个不良事件(QG组:4名受试者发生4次不良事件,安慰剂组:4名受试者发生5次不良事件),均为轻度,与干预无关。临床试验值未见异常变化。在QG组中,注意到组内生化参数的微小变化,但所有值都保持在标准范围内。此外,没有发现显著的组间差异。结论健康成人每日摄入550 mg/d QG,连续4周是安全的,无不良反应,安全参数无临床意义变化。这些发现支持了QG在剂量超过先前测试水平时的安全性。需要进一步的研究来评估长期安全性和更高的剂量。由Aries系统公司的Editorial Manager®和producxion Manager®提供支持。试验注册:临床试验在大学医院医学信息网络临床试验注册中心注册(UMIN- ctr, UMIN ID: UMIN000053632);https://center6.umin.ac.jp/cgi-open-bin/ctr_e/ctr_view.cgi?recptno=R000061206。
{"title":"Safety evaluation of a soluble granular food containing quercetin glycosides for healthy adults: A randomized controlled trial","authors":"Mui Iida ,&nbsp;Misaki Onozato ,&nbsp;Hideyuki Sasaki ,&nbsp;Izumi Yamasaki ,&nbsp;Takao Tanaka ,&nbsp;Hidenori Obata ,&nbsp;Takayuki Izumo ,&nbsp;Yoshihiro Nakao ,&nbsp;Ikuo Fukuhara","doi":"10.1016/j.nfs.2025.100258","DOIUrl":"10.1016/j.nfs.2025.100258","url":null,"abstract":"<div><h3>Background</h3><div>Quercetin glycosides (QG), enzymatically modified isoquercitrin derivatives, are widely used in functional foods for their antioxidant, antihypertensive, and lipid metabolism benefits. Previous safety studies have confirmed tolerability at intake levels up to 480 mg/day for four weeks. However, Japanese regulatory guidelines require overdose safety evaluations at approximately five times the effective dose (110 mg/day), and data on higher intake levels remain limited.</div></div><div><h3>Objective</h3><div>To evaluate the safety of daily consumption of a food product containing 550 mg/day of QG approximately five times the effective dose for four weeks in healthy adults.</div></div><div><h3>Methods</h3><div>A randomized, double-blind, placebo-controlled, parallel-group trial was conducted at a single clinical site in Japan. Healthy adults aged 20–64 years with BMI 18.5–29.9 kg/m<sup>2</sup> were enrolled and randomized (1:1) to receive either soluble granular food containing QG (550 mg/day) or placebo for four weeks. Randomization was stratified by sex, age, and BMI using blocked allocation. Both participants and investigators were blinded to group assignments. The primary endpoint was the incidence of adverse reactions. Secondary endpoints included the incidence of adverse events and changes in physiological and clinical parameters. Safety assessments were performed at baseline, week 2, and week 4.</div></div><div><h3>Results</h3><div>Of 149 screened individuals, 48 were randomized and 47 completed the study(QG group: <em>n</em> = 23, control group: <em>n</em> = 24). No adverse reactions attributable to the test food occurred. Ten adverse events were reported (QG: 4 events in 4 participants, placebo: 5 events in 4 participants), all mild and unrelated to the intervention. In addition, no abnormal changes were observed in clinical test values. In the QG group, minor intragroup changes in biochemical parameters were noted, but all values remained within standard ranges. Also, no significant intergroup differences were detected.</div></div><div><h3>Conclusion</h3><div>Daily intake of 550 mg/day QG for four weeks was safe in healthy adults, with no adverse reactions or clinically meaningful changes in safety parameters. These findings support the safety of QG at doses exceeding previously tested levels. Further research is needed to assess long-term safety and higher dosages. Powered by Editorial Manager® and ProduXion Manager® from Aries Systems Corporation.</div><div>Trial registration: The clinical trial was registration with the University Hospital Medical Information Network Clinical Trials Registry (UMIN-CTR, UMIN ID: UMIN000053632); <span><span>https://center6.umin.ac.jp/cgi-open-bin/ctr_e/ctr_view.cgi?recptno=R000061206</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":19294,"journal":{"name":"NFS Journal","volume":"42 ","pages":"Article 100258"},"PeriodicalIF":3.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145841179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative evaluation of coated and uncoated rainbow trout (Oncorhynchus mykiss) fillets treated with phycocyanin: Antioxidant, antimicrobial, and shelf-life properties 藻蓝蛋白处理虹鳟鱼(Oncorhynchus mykiss)涂膜和未涂膜虹鳟鱼鱼片的比较评价:抗氧化、抗菌和保质期特性
IF 3.3 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-03-01 Epub Date: 2025-12-29 DOI: 10.1016/j.nfs.2025.100259
Bahareh Nowruzi , Seyed Amir Ali Anvar
The rainbow trout (Oncorhynchus mykiss), recognized globally as a widelycultivated fish species, is prized for its exceptional sensory characteristics and nutritional benefits. However, aquaculture products such as trout fillets are often treated with synthetic colorants to enhance visual appeal, raising consumer concerns about their potential toxicity. This has created growing interest in natural, non-toxic alternatives. Phycobiliproteins, particularly phycocyanin (PC) are natural pigments with recognized antimicrobial and antioxidant activity, making them suitable candidates for safer food preservation. The present research evaluated the antimicrobial and antioxidant activity of PC and explored its capability to prolong the freshness and shelf life of trout fillets. Trout samples were treated with PC enriched with citric acid and stored at 4 °C and 8 °C for 21 days. The findings indicated that incorporating both PC and citric acid markedly improved the antioxidant potential of the fillets relative to the untreated controls. Furthermore, the application of PC effectively prolonged the shelf life of rainbow trout fillets to 7 days at 4 °C. Treated fillets remained within acceptable quality limits according to the National Standard of Iran No. 1–2394 for up to 21 days at 4 °C and 14 days at 8 °C, confirming the maintenance of freshness. Sensory evaluation indicated that most attributes of the control fillets, except texture, were acceptable up to day 7. Overall, the use of PC as a natural pigment and bioactive compound presents a promising approach to enhance fish product quality, safety, and shelf life.
虹鳟鱼(Oncorhynchus mykiss)是全球公认的广泛养殖的鱼类,因其独特的感官特征和营养价值而受到重视。然而,鳟鱼鱼片等水产养殖产品通常用合成色素处理,以增强视觉吸引力,这引起了消费者对其潜在毒性的担忧。这引起了人们对天然无毒替代品的兴趣。藻胆蛋白,特别是藻蓝蛋白(PC)是公认的具有抗菌和抗氧化活性的天然色素,使其成为更安全的食品保存的合适人选。本研究评价了PC的抗菌和抗氧化活性,并探讨了其延长鳟鱼鱼片新鲜度和保质期的能力。鳟鱼样品用富含柠檬酸的PC处理,在4°C和8°C保存21天。结果表明,与未处理的对照组相比,添加PC和柠檬酸显著提高了鱼片的抗氧化能力。此外,PC的应用有效地延长了虹鳟鱼片在4°C下的保质期至7天。根据伊朗国家标准No. 1-2394,处理后的鱼片在4°C和8°C条件下可保持21天和14天的可接受质量限制,确认保持新鲜度。感官评价表明,到第7天,除纹理外,对照鱼片的大多数属性都是可以接受的。总之,PC作为一种天然色素和生物活性化合物的使用为提高鱼类产品的质量、安全性和保质期提供了一种很有前途的方法。
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引用次数: 0
Enhancing the characteristics of bitter vetch (Vicia ervilia) starch aerogels through cold plasma treatment and physical cross-linking 通过冷等离子体处理和物理交联提高苦紫薇淀粉气凝胶的特性
IF 3.3 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-03-01 Epub Date: 2026-01-07 DOI: 10.1016/j.nfs.2026.100260
Mahsa Rastehmanfard , Sara Hedayati , Seyed Mohammad Hashem Hosseini , Mahboubeh Fazaeli , Mehrdad Niakousari
This study concentrated on the fabrication and evaluation of four types of aerogels derived from bitter vetch (Vicia ervilia) starch (BVS). These include the conventional native starch aerogel (CNSA), the conventional plasma-treated starch aerogel (CPSA), the physically cross-linked native starch aerogel (PNSA), and the physically cross-linked plasma-treated starch aerogel (PPSA). Cold plasma treatment caused notable modifications in the properties of BVS starch, while leaving its functional groups unchanged, as verified by Raman and Fourier-transform infrared (FT-IR) analysis. Rheological assessments (G′ and G″) indicated that starch treated with plasma developed a weak gel, whereas scanning electron microscopy (SEM) images displayed surface cavities and fissures caused by plasma etching. Despite the crystalline structure being preserved, there was a slight reduction in crystallinity from 21% to 19%. Textural evaluation demonstrated a decrease in springiness following plasma treatment. Subsequent to these processes, aerogels were produced utilizing both native and cold plasma-treated BVS via conventional and physical cross-linking methods. SEM images revealed a porous structure in aerogels that was developed due to water evaporation during the freeze-drying process. FT-IR spectra exhibited the majority of characteristic bands, although some were absent, likely as a result of gelatinization. X-ray diffraction revealed that the aerogels were more amorphous in nature compared to their starch precursors. PPSA demonstrated the highest hardness, the greatest surface area, the least mass loss following a week of water immersion, and exceptional thermal properties. These results indicate that the integration of cold plasma treatment with physical cross-linking markedly enhances the functional and structural attributes of BVS aerogels, highlighting their potential for advanced material applications.
本文主要研究了苦紫薇淀粉(BVS)制备的四种气凝胶的制备及性能评价。这些包括传统的天然淀粉气凝胶(CNSA)、传统的等离子体处理淀粉气凝胶(CPSA)、物理交联的天然淀粉气凝胶(PNSA)和物理交联的等离子体处理淀粉气凝胶(PPSA)。通过拉曼和傅里叶变换红外(FT-IR)分析证实,冷等离子体处理引起了BVS淀粉性质的显著改变,而其官能团不变。流变学评估(G '和G″)表明,等离子体处理的淀粉形成了弱凝胶,而扫描电子显微镜(SEM)图像显示等离子体蚀刻引起的表面空洞和裂缝。尽管晶体结构被保留了下来,但结晶度从21%略微下降到19%。质地评价显示血浆处理后弹性降低。在这些工艺之后,通过常规和物理交联方法,利用原生和冷等离子体处理的BVS生产气凝胶。扫描电镜图像显示气凝胶中的多孔结构是由于冷冻干燥过程中水分蒸发而形成的。FT-IR光谱显示了大多数特征波段,尽管有些特征波段不存在,可能是糊化的结果。x射线衍射显示,与淀粉前体相比,气凝胶在性质上更无定形。PPSA表现出最高的硬度、最大的表面积、浸泡一周后的最小质量损失和优异的热性能。这些结果表明,冷等离子体处理与物理交联的结合显著增强了BVS气凝胶的功能和结构属性,突出了其在先进材料应用中的潜力。
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引用次数: 0
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