NFS Journal最新文献_第3页

Oxidative stability and physical properties of model cream-type O/W emulsions structured with candelilla wax and stored at room temperature 以小蜡烛蜡为原料制备的乳状油/水乳状液模型的氧化稳定性和物理性质

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY

NFS Journal

Pub Date : 2025-06-07 DOI: 10.1016/j.nfs.2025.100233

Iwona Szymańska , Anna Żbikowska , Sylwia Onacik-Gür , Małgorzata Kowalska

Replacing animal-origin products with plant-based raw materials is an environmentally friendly approach and a recommended dietary shift due to its better lipid profile. This study aimed to evaluate the effects of storage time (28 days, 20 °C) on the oxidative stability and physical properties of model cream-type O/W (30/70 m/m) emulsions with candelilla wax-based oleogels (from rapeseed and linseed oils). These emulsions were compared with non-structured emulsions (0 % wax) and a palm oil-based emulsion. The emulsion's primary, secondary, and total degrees of lipid oxidative changes and the microstructure, rheological properties, and physical stability were examined. The oleogelation of the lipid phase in vegan emulsions increased oxidative changes during storage, particularly compared to the palm oil-based emulsion. However, the maximum permissible oxidation levels for refined vegetable oils were not exceeded. The emulsion containing 5 % wax showed the highest oxidative stability among the structured emulsions. Additionally, the changes in the physical parameters of all emulsions were reversible. Emulsions containing 3–5 % wax concentration demonstrated the greatest physical stability among all formulations analyzed. Conversely, 6 or 7 % wax significantly accelerated the emulsions' physical and oxidative destabilization. To preserve the high nutritional value of structured model cream-type emulsions, refrigerated storage and limiting candelilla wax usage to a maximum of 5 % as a stabilizer are suggested. Further research will focus on producing a food product and analyzing its quality and safety, explicitly considering the levels of volatile oxidation products.

用植物性原料代替动物源性产品是一种环保的方法，也是一种推荐的饮食转变，因为它具有更好的脂质特征。本研究旨在评价贮存时间（28天，20°C）对模型奶油型O/W （30/70 m/m）乳剂（从菜籽油和亚麻籽油中提取）的氧化稳定性和物理性能的影响。将这些乳剂与非结构化乳剂（0%蜡）和棕榈油乳剂进行比较。考察了乳状液的一级、二级和总脂质氧化变化程度以及微观结构、流变学性质和物理稳定性。纯素乳剂中脂质相的油凝胶化在储存过程中增加了氧化变化，特别是与棕榈油乳剂相比。然而，精炼植物油的最大允许氧化水平没有超过。在结构乳中，蜡含量为5%的乳状液表现出最高的氧化稳定性。此外，所有乳剂的物理参数变化都是可逆的。在所有分析的配方中，蜡浓度为3 - 5%的乳液表现出最大的物理稳定性。相反，6%或7%的蜡明显加速了乳液的物理和氧化不稳定。为了保持结构模型奶油型乳剂的高营养价值，建议将其冷藏，并将小烛台蜡的用量限制在5%以内作为稳定剂。进一步的研究将集中于生产食品并分析其质量和安全性，明确考虑挥发性氧化产物的水平。

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引用次数: 0

Comparison of the amino acid composition and biological value of protein in fallow deer meat from two farming systems 两种养殖方式休鹿肉中氨基酸组成及蛋白质生物学价值的比较

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY

NFS Journal

Pub Date : 2025-05-23 DOI: 10.1016/j.nfs.2025.100231

Anna Kasprzyk

The primary aim was to determine the protein content, amino acid profile and the biological value of protein in the longissimus lumborum and the semimembranosus muscles of fallow deer originating from an organic system and a conventional system. This is the first study to analyse the amino acid profile and nutritional value of proteins in fallow deer meat from organic farming system. The muscles were collected from 24 fallow deer carcasses with equal proportions of sex and age. The results demonstrated that the follow deer from the organic feeding grounds exhibited higher exogenous and endogenous amino acid contents and nutritional value. A significant effect of the muscle type on the histidine content and the feeding ground × muscle interaction for glycine was noted. In the proteins from fallow deer muscle tissue the content of exogenous amino acids was higher than in the FAO/WHO protein standard. As data regarding fallow deer meat is limited, the results of this study constitute a valuable contribution to knowledge of the meat protein quality. The data presented in this study can be used by dieticians and by breeders. In light of the high quality and nutritional value of this raw material analysed in this paper, it would be advisable to increase its use in the human diet. This meat can be of particular importance in the diets of children and adolescents as well as the elderly and athletes whose protein requirements are elevated.

主要目的是确定有机系统和常规系统的驼鹿腰最长肌和半膜肌中蛋白质含量、氨基酸分布和蛋白质的生物学价值。这是第一个分析有机养殖系统中休闲鹿肉中氨基酸分布和蛋白质营养价值的研究。肌肉取自24具性别、年龄等比例的休鹿尸体。结果表明，有机饲养的鹿茸具有较高的外源和内源氨基酸含量和营养价值。肌肉类型对组氨酸含量和饲粮对甘氨酸的相互作用有显著影响。马鹿肌肉组织蛋白质中外源氨基酸含量高于FAO/WHO蛋白质标准。由于有关休鹿肉的数据有限，本研究的结果对了解休鹿肉的蛋白质质量有重要贡献。本研究提供的数据可供营养学家和育种者使用。鉴于本文所分析的该原料的高品质和营养价值，建议在人类饮食中增加其使用。这种肉在儿童、青少年、老年人和运动员的饮食中尤其重要，因为他们对蛋白质的需求很高。

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引用次数: 0

Essential oil extracted from Hirami lemon (Citrus reticulata var. depressa) agricultural waste promotes mitochondrial biogenesis in C2C12 skeletal muscle cells 从Hirami柠檬（Citrus reticulata vara . depressa）农业废弃物中提取的精油促进C2C12骨骼肌细胞线粒体生物发生

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY

NFS Journal

Pub Date : 2025-05-23 DOI: 10.1016/j.nfs.2025.100232

Chung-Hsuan Wang , Ying-Hao Yeo , Chia-Hsin Lin , Mo-rong Xu , Sheng-Yang Wang

With increasing awareness of the circular economy, extracting valuable components from agricultural waste offers opportunities to create high-value products. Citrus reticulata var. depressa (CD), endemic to Taiwan, produces sour fruits mainly for juice, leaving significant peel waste. In this study, we extract essential oil (CDEO) from CD peels and examines its effects on skeletal muscle energy metabolism. ATP measurements and mitochondrial staining show that CDEO enhances ATP content, mitochondrial membrane potential, and mass in C₂C₁₂ cells. RT-PCR and Western blot analyses indicate that CDEO activates the p-AMPK/SIRT1/PGC-1α pathway and upregulates Nrf1, Nrf2, and TFAM expression. Among the identified constituents, γ-terpinene was found to be the major bioactive compound, capable of promoting ATP production and mitochondrial biogenesis. Future studies should further investigate mitochondrial function and evaluate its anti-fatigue potential using in vivo models.

随着循环经济意识的增强，从农业废弃物中提取有价值的成分为创造高价值产品提供了机会。台湾特有种柑橘（Citrus reticulata var. depressa， CD）产酸果，主要为榨汁用，果皮浪费严重。在这项研究中，我们从CD皮中提取精油（CDEO），并研究其对骨骼肌能量代谢的影响。ATP测量和线粒体染色显示，CDEO提高了C2C12细胞的ATP含量、线粒体膜电位和质量。RT-PCR和Western blot分析表明，CDEO激活p-AMPK/SIRT1/PGC-1α通路，上调Nrf1、Nrf2和TFAM的表达。在鉴定的成分中，γ-萜烯被发现是主要的生物活性化合物，能够促进ATP的产生和线粒体的生物发生。未来的研究应进一步研究线粒体功能，并利用体内模型评估其抗疲劳潜力。

引用次数: 0

Characterization of Fusarium crown rot pathogens and trichothecene genotypes in wheat crops of Hebei province, China 河北省小麦镰刀菌冠腐病病原菌和赤霉病菌基因型分析

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY

NFS Journal

Pub Date : 2025-05-16 DOI: 10.1016/j.nfs.2025.100230

Kahsay Tadesse Mawcha , Mewael Kiros Assefa , Yiying Xu , Na Zhang , Wenxiang Yang , Dennis Ndolo

This study identifies and characterizes pathogenic Fusarium species and their trichothecene genotypes in wheat crops grown in seven regions of Hebei Province, China, from 2019 to 2021. Species-specific primers confirmed the morphological identification of 689 Fusarium strains recovered from wheat seedlings with symptomatic crown/sub-crown tissues. The results suggest that Fusarium pseudograminearum was the most detected strain, accounting for 91 % of the identified strains. Other species, including F. graminearum, F. oxysporum, F. proliferatum, F. asiaticum, and F. culmorum, were detected at lower rates, ranging from 0.15 % to 8.56 %. Toxin genotype detection results showed that F. pseudograminearum and F. graminearum were the most common species linked to wheat crown rot, producing high detection rates of trichothecene genotypes. F. pseudograminearum strains had detection rates of 84.50 % for deoxynivalenol (DON) and other mycotoxins, including 3-acetyl-deoxynivalenol (3-AcDON) and 15-acetyl-deoxynivalenol (15-AcDON). On the other hand, strains (F. graminearum) of the 15-AcDON chemotype were detected at 15 L-AcDON, with detection rates of 34.37 %, 21.88 %, 21.88 %, and 18.75 % in Handan, Shijiazhuang, Xingtai, and Baoding, respectively. The study also indicated that F. pseudograminearum and F. graminearum had strong pathogenicity, while F. asiaticum and F. culmorum showed weak pathogenicity to wheat in Hebei Province. This study identifies F. pseudograminearum as the predominant cause of Fusarium crown rot in Hebei Province, with the majority of strains classified as DON genotypes.

本研究鉴定了2019 - 2021年河北省7个地区小麦作物中致病性镰刀菌及其毛菌基因型。物种特异性引物对689株镰刀菌进行了形态鉴定。结果表明，检出最多的菌株为伪谷物镰刀菌，占检出菌株的91%。其他病原菌的检出率较低，为0.15% ~ 8.56%，分别为谷草镰刀菌、尖孢镰刀菌、增生镰刀菌、亚洲镰刀菌和枯孢镰刀菌。毒素基因型检测结果表明，与小麦冠腐病相关的病原菌中，假谷草镰刀菌和谷草镰刀菌最为常见，产生了较高的毛霉基因型检出率。假谷草镰刀菌对脱氧雪腐镰刀菌醇（DON）和3-乙酰-脱氧雪腐镰刀菌醇（3-AcDON）、15-乙酰-脱氧雪腐镰刀菌醇（15-AcDON）等真菌毒素的检出率为84.50%。在15 L-AcDON处检出15- acdon化学型菌株（F. graminearum），邯郸、石家庄、邢台和保定的检出率分别为34.37%、21.88%、21.88%和18.75%。研究还表明，伪谷粒镰刀菌和谷粒镰刀菌对河北省小麦的致病性较强，而亚洲镰刀菌和culmorum对小麦的致病性较弱。本研究确定了河北省镰刀菌冠腐病的主要病原菌为伪谷粒镰刀菌，大部分菌株为DON基因型。

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引用次数: 0

Fortifying bread rolls with date fruit fiber: Effects on dietary fiber, antioxidant capacity, mineral content, and glycemic response 用枣果纤维强化面包卷：对膳食纤维、抗氧化能力、矿物质含量和血糖反应的影响

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY

NFS Journal

Pub Date : 2025-05-14 DOI: 10.1016/j.nfs.2025.100229

Alaa Almoumen , Huda Mohamed , Athira Subash , Ali H. Al-Marzouqi , Mutamed Ayyash , Ayesha S. Al Dhaheri , Afaf Kamal-Eldin

This study explored the effects of fortifying bread rolls with desugared date fruit pomace on their nutritional value. Bread rolls containing 0, 5, 10, 15, and 20 % date fruit pomace were analyzed for macro- and micronutrients, dietary fiber, phenolic content, and in vitro digestibility. In contrast, three variations (0, 10, and 20 %) were tested for their effect on the glycemic index. Fortification with date fruit pomace significantly increased the total dietary fiber content of the bread, from 5.7 g/100 g in the control to 22.4 g/100 g in the 20 % breads (P < 0.001). Antioxidant activity, indicated by FRAP, rose from 8.2 to 11.1 μmol Trolox equivalents (TE)/g. Similarly, hydrolysable phenolic activity, assessed through both FRAP and DPPH assays, showed an increase with fortification. In vitro digestibility analysis revealed that date pomace fortification progressively reduced glucose release during the oral, gastric, and intestinal digestive phases, with the most significant reduction observed in the oral and gastric phase for the 10 % bread. This effect could result from enzyme inhibition caused by components in date pomace, especially phenolics. Glycemic index testing with eight healthy participants revealed GI values of 93 and 85 for the 10 % and 20 % pomace breads. These results suggest that while desugared date fruit pomace improves the nutritional and antioxidant profile of bread rolls, it has a minimal effect on reducing the glycemic response.

本研究探讨了糖枣果渣强化面包卷对其营养价值的影响。对含有0%、5%、10%、15%和20%枣渣的面包卷进行了宏量营养素和微量营养素、膳食纤维、酚类含量和体外消化率的分析。相比之下，测试了三种变化（0%、10%和20%）对血糖指数的影响。添加枣果渣的强化显著提高了面包的总膳食纤维含量，从对照组的5.7 g/100 g增加到20%面包的22.4 g/100 g (P <；0.001)。FRAP测定抗氧化活性由8.2 μmol Trolox当量（TE /g）提高到11.1 μmol Trolox当量（TE /g）。同样，通过FRAP和DPPH测定，水解酚活性也随着强化而增加。体外消化率分析显示，强化枣渣逐渐减少了口服、胃和肠道消化阶段的葡萄糖释放，其中10%面包在口服和胃消化阶段的减少最为显著。这种影响可能是由于枣渣中成分，特别是酚类物质对酶的抑制作用所致。对8名健康参与者进行的血糖指数测试显示，10%和20%果渣面包的GI值分别为93和85。这些结果表明，虽然糖枣果渣改善了面包卷的营养和抗氧化特性，但它对降低血糖反应的作用很小。

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引用次数: 0

Optimisation of extraction method for quantification of free fatty acids in oat-, pea- and faba-bean-based protein sources by liquid chromatography coupled with mass spectrometry 液相色谱-质谱联用法测定燕麦、豌豆和蚕豆蛋白源中游离脂肪酸的提取方法优化

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY

NFS Journal

Pub Date : 2025-05-06 DOI: 10.1016/j.nfs.2025.100228

Anastassia Zeinatulina , Aleksei Kaleda , Maria Kuhtinskaja , Tiina Kriščiunaite

Plant-based protein sources, including oat, pea, and faba bean, are widely used in milk and meat alternatives. However, these crops can be inherently bitter, which limits their use in some food products. Processing into flours, protein concentrates, and isolates may cause changes in chemical composition and influence bitterness. We present an optimised and validated LC-MS method using isopropanol:methanol (1:1, v/v) solvent extraction to determine selected bitter-tasting free fatty acids (FFAs). Accurate quantification of FFAs (linolenic, myristic, palmitic, linoleic, oleic, and stearic acids) was achieved using isotopically labelled oat flour extract as an internal standard. The total FFA content in whole grain oat flour, oat protein concentrate, pea flour and protein isolate, and faba bean flour and protein isolate ranged from 4.4 to 3841 mg/100 g dry weight. FFA analysis aids in assessing bitterness and selecting processing techniques to reduce these compounds in plant-based foods.

植物性蛋白质来源，包括燕麦、豌豆和蚕豆，被广泛用于牛奶和肉类替代品。然而，这些作物本身就很苦，这限制了它们在某些食品中的使用。加工成面粉、蛋白质浓缩物和分离物可能会导致化学成分的变化并影响苦味。我们提出了一种优化和验证的LC-MS方法，使用异丙醇：甲醇（1:1,v/v）溶剂萃取来测定选定的苦味游离脂肪酸（FFAs）。使用同位素标记的燕麦面粉提取物作为内标，实现了FFAs（亚麻酸、肉豆蔻酸、棕榈酸、亚油酸、油酸和硬脂酸）的准确定量。全谷物燕麦粉、燕麦浓缩蛋白、豌豆粉和分离蛋白、蚕豆粉和分离蛋白的总游离脂肪酸含量为4.4 ~ 3841 mg/100 g干重。FFA分析有助于评估植物性食品中的苦味和选择加工技术以减少这些化合物。

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引用次数: 0

Encapsulation of mango peel bioactive compounds in milk, gum acacia, and maltodextrin improves its stability 芒果皮生物活性化合物在牛奶、金合树胶和麦芽糖糊精中的包封提高了其稳定性

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY

NFS Journal

Pub Date : 2025-04-30 DOI: 10.1016/j.nfs.2025.100227

Mahbuba Rahman Tripty, Asmaul Husna Nupur, Jannatul Ferdushi Jany, Maria Afroz Toma, Md. Anisur Rahman Mazumder

There is an increased interest and need to make our economy more circular and our diets healthier and more sustainable. Mango peel is often discarded as waste after consuming mango pulp, despite its excellent source of bioactive compounds, especially phenolic compounds. The aim of this study was to develop encapsulated milk powder containing mango peel polyphenol (MPP) and assess the effects of MPP on the physicochemical properties, antioxidant activity, sensory and storage stability of milk powder. MPP was encapsulated with milk, gum acacia (4, 6 and 8 % w/v), and maltodextrin (10 % w/v) using spray drying and cabinet drying. Nutritional analysis revealed that ripe mango peel powder provide total polyphenol of 64.37 ± 0.26 mg GAE/g, total flavonoid of 10.73 ± 0.11 (mg QuE/g), and antioxidant activity (DPPH) of 52.56 %. Regarding the physico-chemical characteristics of the MPP encapsulated spray-dried milk powder showed increases (p < 0.05) in bulk density, flowability, cohesiveness, hygroscopicity, and solubility, whereas cabinet-dried milk powder showed increases (p < 0.05) in moisture content and tapped density. Spray-dried milk powder had greater retention (p < 0.05) of color parameters and retained high phenolic compounds during storage at 4 or 25 °C for 60 days compared to cabinet-dried milk powder. No significant differences (p > 0.05) were observed between 4 % and 6 % w/v gum acacia concentrations in terms of encapsulation efficiency, physico-chemical properties and color retention. The results show that MPP polyphenol can be encapsulated by combining gum acacia (4 or 6 %), 10 % maltodextrin, and milk powder at 4 °C for longer shelf life.

让我们的经济更循环，让我们的饮食更健康、更可持续，人们的兴趣和需求都在增加。尽管芒果皮富含生物活性化合物，尤其是酚类化合物，但在食用芒果果肉后，芒果皮往往被作为废物丢弃。本研究旨在研制芒果皮多酚（MPP）胶囊奶粉，并考察MPP对奶粉理化性质、抗氧化活性、感官及贮存稳定性的影响。采用喷雾干燥和柜式干燥，用牛奶、金合木（4、6和8% w/v）和麦芽糖糊精（10% w/v）包封MPP。营养分析表明，成熟芒果果皮粉的总多酚含量为64.37±0.26 mg GAE/g，总黄酮含量为10.73±0.11 (mg QuE/g)，抗氧化活性（DPPH）为52.56%。在物理化学特性方面，MPP包封后的喷雾干燥奶粉表现出(p <；在容重、流动性、黏结性、吸湿性和溶解度等方面均有显著提高(p <；0.05)的含水率和出丝密度。喷雾干燥奶粉的保留率更高(p <；与柜干奶粉相比，在4°C或25°C条件下保存60天，其颜色参数和保留的高酚类化合物的差异为0.05)。无显著差异(p >；在w/v浓度为4% ~ 6%时，相思胶的包封率、理化性质和保色性均显著高于0.05)。结果表明，在4℃条件下，将相思胶（4%或6%）、10%麦芽糖糊精和奶粉混合可包封MPP多酚，延长了MPP多酚的保质期。

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引用次数: 0

Jatrorrhizine, an alkaloid isolated from Phellodendron amurense, reduces melanogenesis in mouse B16F10 melanoma cells 黄藤根碱是一种从黄柏中分离出来的生物碱，可减少小鼠B16F10黑色素瘤细胞的黑色素生成

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY

NFS Journal

Pub Date : 2025-03-01 DOI: 10.1016/j.nfs.2025.100221

Junhyo Cho , Eun-Ho Lee , Young-Je Cho , Yeonhwa Park

Jatrorrhizine isolated from Phellodendron amurense has been reported as one of the major phenolic alkaloid compounds. Previous studies indicated that jatrorrhizine has potential health benefits. In this study, we used jatrorrhizine isolated from P. amurense to determine its effects on melanogenesis using B16F10 mouse melanoma cells. Jatrorrhizine at 20–100 μM inhibited α-melanocyte-stimulating hormone-induced melanin biosynthesis without cytotoxicity. Treatment with jatrorrhizine inhibited melanocortin 1 receptor, increased transforming growth factor-β1, and inhibited microphthalmia transcription factor, tyrosinase-related protein-1, tyrosinase-related protein-2, tyrosinase expression, and tyrosinase activity compared to the α-MSH-treated control. In addition, treatment with jatrorrhizine inhibited RAB27A and MYO5A, which are key components of melanosome transport. The current results suggest that jatrorrhizine has the potential to be used in functional cosmetics and beauty foods to improve skin tone. Further in vivo studies using jatrorrhizine would be needed to support the current observations.

从黄柏中分离得到的黄根碱是黄柏中主要的酚类生物碱化合物之一。以前的研究表明，黄麻根碱具有潜在的健康益处。在这项研究中，我们使用从麻疯树中分离的麻疯根碱来研究其对B16F10小鼠黑色素瘤细胞黑色素生成的影响。在20 ~ 100 μM的剂量下，麻草酸对α-黑色素细胞刺激激素诱导的黑色素生物合成有抑制作用，但无细胞毒性。与α- msh对照相比，麻草酸根碱抑制黑素皮质素1受体，增加转化生长因子-β1，抑制小眼转录因子、酪氨酸酶相关蛋白-1、酪氨酸酶相关蛋白-2、酪氨酸酶表达和酪氨酸酶活性。此外，麻根碱处理抑制了黑色素小体运输的关键成分RAB27A和MYO5A。目前的研究结果表明，黄根碱有潜力用于功能性化妆品和美容食品中，以改善肤色。需要进一步使用麻风根碱进行体内研究来支持目前的观察结果。

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引用次数: 0

Tissue distribution and accumulation of cinnamic acid derivatives from Brazilian green propolis in mice 巴西绿蜂胶肉桂酸衍生物在小鼠体内的组织分布和积累

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY

NFS Journal

Pub Date : 2025-03-01 DOI: 10.1016/j.nfs.2025.100222

Masayuki Yamaga , Masakatsu Nohara , Akihisa Hata , Takashi Ito , Kayo Furumoto , Naomi Ohta , Jiro Miyamae , Hiroko Tani , Ayanori Yamaki , Noboru Fujitani

Cinnamic acid derivatives, particularly prenylated derivatives, are characteristic components of Brazilian green propolis (BGP) and have been attracting attention for their health benefits. However, few reports have investigated their tissue distribution. This study aimed to elucidate the tissue distribution of cinnamic acid derivatives and examine the effects of prenylation by administering BGP to mice. The distribution level of p-coumaric acid was higher in the kidneys than in the liver or intestine. Artepillin C was detected at higher levels in the liver and intestine than in the kidney, and was also distributed in the brain, muscle, spleen, pancreas, and adipose tissue. These results suggested that prenylation enhanced the tissue uptake of cinnamic acid derivatives, which may facilitate the understanding of their functional mechanisms and promote their use in pharmaceuticals and functional foods.

肉桂酸衍生物，特别是烯酰化衍生物，是巴西绿蜂胶（BGP）的特色成分，因其对健康的益处而备受关注。然而，研究其组织分布的报道很少。本研究旨在阐明肉桂酸衍生物的组织分布，并通过给药BGP检测戊烯酰化对小鼠的影响。对香豆酸在肾脏中的分布水平高于肝脏和肠道。肝、肠中Artepillin C含量高于肾，在脑、肌肉、脾、胰腺和脂肪组织中也有分布。这些结果表明，戊烯酰化促进了肉桂酸衍生物的组织吸收，这可能有助于了解其功能机制，并促进其在药物和功能食品中的应用。

引用次数: 0

High-pressure processing (HPP) alters tetracycline resistance in Listeria monocytogenes: A phenotypic and genotypic study 高压处理（HPP）改变单核增生李斯特菌的四环素耐药性：一项表型和基因型研究

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY

NFS Journal

Pub Date : 2025-03-01 DOI: 10.1016/j.nfs.2025.100223

Patryk Adamski, Arkadiusz Zakrzewski, Patryk Wiśniewski, Wioleta Chajęcka-Wierzchowska, Anna Zadernowska

This study insights into the effects of high-pressure processing (HPP) on the phenotypic resistance and expression of tetracycline resistance genes (tetA_1, tetA_2, tetA_3, tetC, tetR) in Listeria monocytogenes strains. A total of 29 strains were tested for minimum inhibitory concentration values (MIC) of tetracycline before and after HPP treatment (200 MPa/5 min). Results showed a significant increase in MIC values post-treatment, with 7 % of the strains exhibiting resistance. Gene expression analysis revealed overexpression of tetA_1 and tetA_2 genes, while tetA_3, tetC, and tetR showed varied responses, suggesting a strain specific expression pattern. These findings indicate that HPP induced stress may influence the development of antibiotic resistance in L. monocytogenes, highlighting the importance of monitoring resistance genes in food safety protocols.

本研究探讨了高压处理（HPP）对单核增生李斯特菌表型抗性及四环素抗性基因（tetA_1、tetA_2、tetA_3、tetC、tetR）表达的影响。检测29株菌株在HPP处理（200 MPa/5 min）前后对四环素的最低抑菌浓度（MIC）。结果显示，处理后的MIC值显著增加，7%的菌株表现出耐药性。基因表达分析显示tetA_1和tetA_2基因过表达，而tetA_3、tetC和tetR基因表现出不同的表达，提示菌株特异性表达模式。这些研究结果表明，HPP诱导的应激可能影响单核增生乳杆菌的耐药性发展，强调了在食品安全方案中监测抗性基因的重要性。

引用次数: 0