NFS Journal最新文献

英文中文

Corrigendum to “Bulgarian yogurt relieved symptoms and distress and increased fecal short-chain fatty acids in healthy constipated women: A randomized, blinded crossover controlled trial” [NFS Journal, Volume 22, March 2021, Pages 20–31] “保加利亚酸奶缓解了健康便秘妇女的症状和痛苦，增加了粪便短链脂肪酸：一项随机、盲法交叉对照试验”的勘误表[NFS Journal， vol . 22, March 2021, Pages 20-31]

IF 3.3 Q2 FOOD SCIENCE & TECHNOLOGY

NFS Journal

Pub Date : 2025-08-23 DOI: 10.1016/j.nfs.2025.100243

Rata Khuropakhonphong , Kanyawee Whanmek , Sasiumphai Purttiponthanee , Wimonphan Chathiran , Warangkana Srichamnong , Chalat Santivarangkna , Dunyaporn Trachootham

引用次数: 0

Protective effects of immature Citrus nippokoreana fruit extract on TNF-α/IFN-γ-induced intestinal epithelial barrier disruption 鲜柑桔果实提取物对TNF-α/IFN-γ诱导的肠上皮屏障破坏的保护作用

IF 3.3 Q2 FOOD SCIENCE & TECHNOLOGY

NFS Journal

Pub Date : 2025-08-21 DOI: 10.1016/j.nfs.2025.100242

Ulfah Dwi Kurniawati , Chi Heung Cho , Young Sung Jung , Mingyeong Kim , Ho-Young Park , Sang-Hoon Lee

This study investigated the protective effects of immature Citrus nippokoreana fruit extract (CFE) against tumor necrosis factor (TNF)-α/interferon (IFN)-γ-induced barrier disruption in human intestinal epithelial cells. CFE demonstrated anti-inflammatory effects by suppressing the increased production of the proinflammatory cytokines interleukin (IL)-1β, IL-6, IL-8, and monocyte chemoattractant protein (MCP)-1. Moreover, CFE maintained barrier integrity, which was evidenced by its ability to prevent reductions in trans-epithelial electrical resistance (TEER) and increments in fluorescein isothiocyanate (FITC)-dextran permeability. The protective activity of CFE was supported by the preservation of the expression and localization of the tight junction proteins zonula occludens (ZO)-1, occludin, and claudin-1. CFE prevented nuclear factor (NF)-κB activation and translocation, myosin light chain kinase (MLCK) upregulation, and myosin light chain II (MLC-2) phosphorylation. The presence of flavonoids, namely didymin, sinensetin, eriocitrin, naringin, narirutin, and hesperidin, was confirmed in CFE. These findings indicate that CFE can serve as a protective agent for maintaining intestinal barrier integrity by suppressing inflammation and preserving tight junctions.

本研究探讨了柑桔未成熟果实提取物（CFE）对肿瘤坏死因子(TNF)-α/干扰素(IFN)-γ-诱导的人肠上皮细胞屏障破坏的保护作用。CFE通过抑制促炎细胞因子白细胞介素(IL)-1β、IL-6、IL-8和单核细胞化学引诱蛋白(MCP)-1的增加而显示出抗炎作用。此外，CFE保持了屏障的完整性，这可以通过其防止跨上皮电阻（TEER）的降低和异硫氰酸荧光素(FITC)-葡聚糖渗透性的增加来证明。CFE的保护作用是由于紧密连接蛋白ZO -1、occludin和claudin-1的表达和定位得以保存。CFE可抑制核因子(NF)-κB活化和易位、肌球蛋白轻链激酶（MLCK）上调和肌球蛋白轻链II （MLC-2）磷酸化。在CFE中证实了黄酮类化合物的存在，即双草胺、三叶草苷、苦皮苷、柚皮苷、narirutin和橙皮苷。这些发现表明，CFE可以作为一种保护剂，通过抑制炎症和保持紧密连接来维持肠道屏障的完整性。

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引用次数: 0

Exploring the pros and cons of plant-based egg alternatives – A review 探索植物性鸡蛋替代品的利弊-综述

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY

NFS Journal

Pub Date : 2025-07-19 DOI: 10.1016/j.nfs.2025.100241

Ahmadullah Zahir , Shazia Akhter

The global population is projected to surpass 10 billion by 2050, intensifying the demand for agricultural food production to nourish this expanding number. Amidst rising climate change, pollution, and global crises, the increasing need for plant-based food items, including plant-based eggs, presents a viable path for environmental protection and health enhancement, fostering the development and adoption of plant-based egg alternatives. The food industry's growing interest in these egg substitutes is influenced by several factors, including consumer preference, reduced allergen content, improved food safety, healthier nutritional profiles, greater ease of handling and storage, enhanced functionality, cost-effectiveness with reduced price volatility, and a focus on environmental sustainability. These alternatives are commonly derived from various plant-based ingredients, including mung beans, chickpeas, soy, and various starches. Despite the market's expansion, significant challenges for researchers include ensuring the versatility and superior rheological and nutritional properties of these substitutes. Additionally, it is crucial to consider antinutritional factors and allergens present in the plant compounds used for egg alternatives when developing these products. This review will assess the advantages and disadvantages of plant-based egg substitutes, examining key aspects, including nutritional value, environmental impact, taste and texture, cost, and their effect on consumer acceptance and overall health.

到2050年，全球人口预计将超过100亿，这将加大对农业粮食生产的需求，以养活这一不断增长的人口。随着气候变化、污染和全球危机的加剧，对植物性食品（包括植物性鸡蛋）的需求日益增加，为环境保护和增进健康提供了一条可行的途径，促进了植物性鸡蛋替代品的开发和采用。食品行业对这些鸡蛋替代品日益增长的兴趣受到几个因素的影响，包括消费者偏好、减少过敏原含量、提高食品安全、更健康的营养状况、更容易处理和储存、增强功能、降低价格波动的成本效益，以及对环境可持续性的关注。这些替代品通常来源于各种植物性成分，包括绿豆、鹰嘴豆、大豆和各种淀粉。尽管市场在扩大，但研究人员面临的重大挑战包括确保这些替代品的多功能性和优越的流变学和营养特性。此外，在开发这些产品时，考虑用于鸡蛋替代品的植物化合物中存在的抗营养因子和过敏原是至关重要的。本综述将评估植物性鸡蛋替代品的优缺点，审查关键方面，包括营养价值、环境影响、味道和质地、成本以及它们对消费者接受度和整体健康的影响。

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引用次数: 0

Comparing methods for the analysis of peptides from in vitro digestion for evaluating the digestibility of plant proteins 比较体外消化多肽分析方法对植物蛋白消化率的评价

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY

NFS Journal

Pub Date : 2025-07-11 DOI: 10.1016/j.nfs.2025.100240

Benno F. Zimmermann , Eva Maria Daniels , Marieke Katharina Leurs , Linus Rode , Ute Schweiggert-Weisz

In this article, two analytical topics and one nutritional topic are covered. 1. Analytical: the effects of formic acid and trifluoroacetic acid (TFA) at different concentrations in size exclusion chromatography (SEC) for peptide analysis were evaluated. 2. Analytical: three methods for the analysis of peptides after in vitro digestion according to the INFOGEST protocol were compared: photometric quantification after OPA derivatization, SDS–PAGE and SEC. 3. Nutritional: using the most suitable and optimized analytical method, the digestibility of nine protein concentrates from plants and two animal proteins was evaluated.

The results are as follows: 1. Alternative additives or low TFA concentrations compromise peak resolution. 2. When the digestibility of proteins is evaluated, it is necessary to determine the small peptides that are bioaccessible. This was possible only by SEC. 3. The digestibility of plant proteins – as determined by SEC after in vitro digestion – was found to be in the same range or higher than that of animal proteins.

在这篇文章中，包括两个分析主题和一个营养主题。1. 分析：评价了不同浓度的甲酸和三氟乙酸（TFA）在尺寸排除色谱（SEC）中对多肽分析的影响。2. 分析：根据INFOGEST方案，比较了三种分析体外消化后肽的方法：OPA衍生化后的光度法、SDS-PAGE法和SEC法。营养方面：采用最适合和优化的分析方法，对9种植物浓缩蛋白和2种动物浓缩蛋白的消化率进行了评价。研究结果如下：1。替代添加剂或低TFA浓度损害峰分辨率。2. 当评估蛋白质的消化率时，有必要确定生物可及的小肽。这只有通过第3条才有可能。植物蛋白的消化率——用体外消化后的SEC测定——与动物蛋白的消化率相同或更高。

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引用次数: 0

A comparative review on novel-assisted extraction techniques for retrieving protein from some potential plant sources 从一些潜在植物源中提取蛋白质的新辅助提取技术的比较综述

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY

NFS Journal

Pub Date : 2025-06-26 DOI: 10.1016/j.nfs.2025.100239

Paul Ndubuisi Anyiam , Suphat Phongthai , Passakorn Kingwascharapong , Jaksuma Pongsetkul , Wanli Zhang , Young Hoon Jung , Saroat Rawdkuen

Global protein demand is rising as populations grow and consumers seek sustainable and nutritious alternatives. Plant-based proteins are favored for their consumer acceptance, lower environmental impact, and health benefits. However, the main challenge in extracting high-quality and functional plant proteins for food applications is to select the most suitable extraction method. Conventional protein extraction methods (acid-alkaline and salt extraction) have several drawbacks. Novel-assisted extraction techniques such as enzyme-assisted, ultrasound-assisted, microwave-assisted, high-pressure-assisted, and pulse electric field-assisted extractions, have proven effective in enhancing the yield of high-quality protein. This review examines recent data on novel protein extraction technologies from six promising plant protein sources under optimized conditions, focusing on their yield, protein quality, and functional properties, as well as mechanisms, drawbacks and future perspectives compared to conventional techniques. The final section explores recent trends in enriching foods with protein isolated from some potential plant sources, highlighting their ability to improve food quality and tackle protein deficiencies. The available data indicate that under optimized conditions, cell-disruptive techniques have the potential to improve protein recovery, and functional properties from plant sources, while minimizing environmental pollution. Given the recent surge in interest in alternative plant-based proteins, this review will offer insights and references for future research and development in plant-based protein processing.

随着人口增长和消费者寻求可持续和有营养的替代品，全球蛋白质需求正在上升。基于植物的蛋白质因其消费者接受度，对环境影响较小和健康益处而受到青睐。然而，为食品应用提取高质量和功能性植物蛋白的主要挑战是选择最合适的提取方法。传统的蛋白质提取方法（酸碱和盐提取）有几个缺点。新的辅助提取技术，如酶辅助、超声辅助、微波辅助、高压辅助和脉冲电场辅助提取，已被证明在提高高质量蛋白质的产量方面是有效的。本文综述了在优化条件下从六种有前途的植物蛋白来源中提取蛋白质的新技术的最新数据，重点介绍了它们的产量、蛋白质质量和功能特性，以及与传统技术相比的机制、缺点和未来展望。最后一节探讨了用从某些潜在植物来源分离的蛋白质来丰富食品的最新趋势，强调了它们提高食品质量和解决蛋白质缺乏问题的能力。现有的数据表明，在优化的条件下，细胞破坏技术有可能提高蛋白质的回收率和植物来源的功能特性，同时最大限度地减少环境污染。鉴于近年来对替代植物蛋白的兴趣激增，本文综述将为植物蛋白加工的未来研究和发展提供见解和参考。

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引用次数: 0

Editorial: Food-to-food fortification: Where food science and nutrition join forces to create locally sustainable and effective nutritional solutions 社论：食品到食品强化：食品科学和营养联合起来创造当地可持续和有效的营养解决方案

IF 3.3 Q2 FOOD SCIENCE & TECHNOLOGY

NFS Journal

Pub Date : 2025-06-13 DOI: 10.1016/j.nfs.2025.100234

Johanita Kruger , Mario Ferruzzi

引用次数: 0

Effects of plasmin on camel and bovine model cheeses: Protein degradation, texture, and rheology 纤溶蛋白对骆驼和牛模型奶酪的影响：蛋白质降解、质地和流变学

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY

NFS Journal

Pub Date : 2025-06-13 DOI: 10.1016/j.nfs.2025.100235

Santhoshani Warakaulle , Delphine Vincent , Basim Abu-Jdayil , Mutamed M. Ayyash , Afaf Kamal-Eldin

This study investigated the effects of plasmin addition on the physicochemical, textural, and rheological properties throughout the ripening process of cheeses made of camel and bovine milk. Model cheeses were produced with varying plasmin concentrations (0, 0.15, 0.3, and 0.6 U/100 mL milk) to assess changes in physicochemical attributes, casein hydrolysis, hardness, and rheological behavior. Three-way ANOVA demonstrated that the type of milk, plasmin level, ripening duration, and their interactions significantly influenced titratable acidity (%), pH, concentrations of free amino groups, and hardness (p < 0.05). Urea-PAGE analysis revealed changes in CM and BM cheeses during storage, most notably a reduction in the intensity of the β-casein band indicating progressive proteolysis. Ripening resulted in elevated concentrations of free amino groups in both camel and bovine milk cheeses (p < 0.05). The proteolysis of casein during ripening, subsequently leads to increased water retention, which contributes to softer cheese and reduction in both G´ and G´´ values over time.

本研究考察了在驼奶和牛奶制成的奶酪成熟过程中，加入纤溶酶对其理化、质地和流变学特性的影响。用不同的纤溶酶浓度（0、0.15、0.3和0.6 U/100 mL牛奶）制作模型奶酪，以评估其理化属性、酪蛋白水解、硬度和流变行为的变化。三因素方差分析表明，牛奶类型、血浆蛋白水平、成熟时间及其相互作用显著影响可滴定酸度（%）、pH、游离氨基浓度和硬度(p <；0.05)。尿素- page分析显示CM和BM奶酪在储存过程中发生了变化，最明显的是β-酪蛋白带强度的降低，表明蛋白质水解正在进行。成熟导致骆驼奶和牛奶奶酪中游离氨基浓度升高(p <；0.05)。成熟过程中酪蛋白的蛋白质水解，随后导致水分潴留增加，这有助于奶酪变软，并随着时间的推移降低G ‘和G ’值。

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引用次数: 0

Oxidative stability and physical properties of model cream-type O/W emulsions structured with candelilla wax and stored at room temperature 以小蜡烛蜡为原料制备的乳状油/水乳状液模型的氧化稳定性和物理性质

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY

NFS Journal

Pub Date : 2025-06-07 DOI: 10.1016/j.nfs.2025.100233

Iwona Szymańska , Anna Żbikowska , Sylwia Onacik-Gür , Małgorzata Kowalska

Replacing animal-origin products with plant-based raw materials is an environmentally friendly approach and a recommended dietary shift due to its better lipid profile. This study aimed to evaluate the effects of storage time (28 days, 20 °C) on the oxidative stability and physical properties of model cream-type O/W (30/70 m/m) emulsions with candelilla wax-based oleogels (from rapeseed and linseed oils). These emulsions were compared with non-structured emulsions (0 % wax) and a palm oil-based emulsion. The emulsion's primary, secondary, and total degrees of lipid oxidative changes and the microstructure, rheological properties, and physical stability were examined. The oleogelation of the lipid phase in vegan emulsions increased oxidative changes during storage, particularly compared to the palm oil-based emulsion. However, the maximum permissible oxidation levels for refined vegetable oils were not exceeded. The emulsion containing 5 % wax showed the highest oxidative stability among the structured emulsions. Additionally, the changes in the physical parameters of all emulsions were reversible. Emulsions containing 3–5 % wax concentration demonstrated the greatest physical stability among all formulations analyzed. Conversely, 6 or 7 % wax significantly accelerated the emulsions' physical and oxidative destabilization. To preserve the high nutritional value of structured model cream-type emulsions, refrigerated storage and limiting candelilla wax usage to a maximum of 5 % as a stabilizer are suggested. Further research will focus on producing a food product and analyzing its quality and safety, explicitly considering the levels of volatile oxidation products.

用植物性原料代替动物源性产品是一种环保的方法，也是一种推荐的饮食转变，因为它具有更好的脂质特征。本研究旨在评价贮存时间（28天，20°C）对模型奶油型O/W （30/70 m/m）乳剂（从菜籽油和亚麻籽油中提取）的氧化稳定性和物理性能的影响。将这些乳剂与非结构化乳剂（0%蜡）和棕榈油乳剂进行比较。考察了乳状液的一级、二级和总脂质氧化变化程度以及微观结构、流变学性质和物理稳定性。纯素乳剂中脂质相的油凝胶化在储存过程中增加了氧化变化，特别是与棕榈油乳剂相比。然而，精炼植物油的最大允许氧化水平没有超过。在结构乳中，蜡含量为5%的乳状液表现出最高的氧化稳定性。此外，所有乳剂的物理参数变化都是可逆的。在所有分析的配方中，蜡浓度为3 - 5%的乳液表现出最大的物理稳定性。相反，6%或7%的蜡明显加速了乳液的物理和氧化不稳定。为了保持结构模型奶油型乳剂的高营养价值，建议将其冷藏，并将小烛台蜡的用量限制在5%以内作为稳定剂。进一步的研究将集中于生产食品并分析其质量和安全性，明确考虑挥发性氧化产物的水平。

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引用次数: 0

Comparison of the amino acid composition and biological value of protein in fallow deer meat from two farming systems 两种养殖方式休鹿肉中氨基酸组成及蛋白质生物学价值的比较

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY

NFS Journal

Pub Date : 2025-05-23 DOI: 10.1016/j.nfs.2025.100231

Anna Kasprzyk

The primary aim was to determine the protein content, amino acid profile and the biological value of protein in the longissimus lumborum and the semimembranosus muscles of fallow deer originating from an organic system and a conventional system. This is the first study to analyse the amino acid profile and nutritional value of proteins in fallow deer meat from organic farming system. The muscles were collected from 24 fallow deer carcasses with equal proportions of sex and age. The results demonstrated that the follow deer from the organic feeding grounds exhibited higher exogenous and endogenous amino acid contents and nutritional value. A significant effect of the muscle type on the histidine content and the feeding ground × muscle interaction for glycine was noted. In the proteins from fallow deer muscle tissue the content of exogenous amino acids was higher than in the FAO/WHO protein standard. As data regarding fallow deer meat is limited, the results of this study constitute a valuable contribution to knowledge of the meat protein quality. The data presented in this study can be used by dieticians and by breeders. In light of the high quality and nutritional value of this raw material analysed in this paper, it would be advisable to increase its use in the human diet. This meat can be of particular importance in the diets of children and adolescents as well as the elderly and athletes whose protein requirements are elevated.

主要目的是确定有机系统和常规系统的驼鹿腰最长肌和半膜肌中蛋白质含量、氨基酸分布和蛋白质的生物学价值。这是第一个分析有机养殖系统中休闲鹿肉中氨基酸分布和蛋白质营养价值的研究。肌肉取自24具性别、年龄等比例的休鹿尸体。结果表明，有机饲养的鹿茸具有较高的外源和内源氨基酸含量和营养价值。肌肉类型对组氨酸含量和饲粮对甘氨酸的相互作用有显著影响。马鹿肌肉组织蛋白质中外源氨基酸含量高于FAO/WHO蛋白质标准。由于有关休鹿肉的数据有限，本研究的结果对了解休鹿肉的蛋白质质量有重要贡献。本研究提供的数据可供营养学家和育种者使用。鉴于本文所分析的该原料的高品质和营养价值，建议在人类饮食中增加其使用。这种肉在儿童、青少年、老年人和运动员的饮食中尤其重要，因为他们对蛋白质的需求很高。

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引用次数: 0

Essential oil extracted from Hirami lemon (Citrus reticulata var. depressa) agricultural waste promotes mitochondrial biogenesis in C2C12 skeletal muscle cells 从Hirami柠檬（Citrus reticulata vara . depressa）农业废弃物中提取的精油促进C2C12骨骼肌细胞线粒体生物发生

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY

NFS Journal

Pub Date : 2025-05-23 DOI: 10.1016/j.nfs.2025.100232

Chung-Hsuan Wang , Ying-Hao Yeo , Chia-Hsin Lin , Mo-rong Xu , Sheng-Yang Wang

With increasing awareness of the circular economy, extracting valuable components from agricultural waste offers opportunities to create high-value products. Citrus reticulata var. depressa (CD), endemic to Taiwan, produces sour fruits mainly for juice, leaving significant peel waste. In this study, we extract essential oil (CDEO) from CD peels and examines its effects on skeletal muscle energy metabolism. ATP measurements and mitochondrial staining show that CDEO enhances ATP content, mitochondrial membrane potential, and mass in C₂C₁₂ cells. RT-PCR and Western blot analyses indicate that CDEO activates the p-AMPK/SIRT1/PGC-1α pathway and upregulates Nrf1, Nrf2, and TFAM expression. Among the identified constituents, γ-terpinene was found to be the major bioactive compound, capable of promoting ATP production and mitochondrial biogenesis. Future studies should further investigate mitochondrial function and evaluate its anti-fatigue potential using in vivo models.

随着循环经济意识的增强，从农业废弃物中提取有价值的成分为创造高价值产品提供了机会。台湾特有种柑橘（Citrus reticulata var. depressa， CD）产酸果，主要为榨汁用，果皮浪费严重。在这项研究中，我们从CD皮中提取精油（CDEO），并研究其对骨骼肌能量代谢的影响。ATP测量和线粒体染色显示，CDEO提高了C2C12细胞的ATP含量、线粒体膜电位和质量。RT-PCR和Western blot分析表明，CDEO激活p-AMPK/SIRT1/PGC-1α通路，上调Nrf1、Nrf2和TFAM的表达。在鉴定的成分中，γ-萜烯被发现是主要的生物活性化合物，能够促进ATP的产生和线粒体的生物发生。未来的研究应进一步研究线粒体功能，并利用体内模型评估其抗疲劳潜力。

引用次数: 0

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