Suluh NORMASIWI, Siti S. HAFIZHAH, Ida ADVIANY, Muhammad I. SURYA
Prunus cerasoides have a high value in phytochemistry and pharmacology. It was classified as the Least Concern globally based on the IUCN red list due to its widespread distribution in eastern Asia. This research aims to evaluate the reproductive biology of P. cerasoides through the study of pollen morphology, pollen viability, stigma receptivity, and pollination in the Cibodas Botanical Garden, located in the Cibodas subdistrict of West Java, Indonesia. The pollen morphology was observed using SEM. Moreover, the pollen viability test was followed by the staining method (aceto-orcein 2%, I2KI 1%, TTC 1%) and in vitro pollen germination with thirteen treatments (aquadest [control]; 5-30% sucrose; and 5-30% sucrose + 5 ppm boric acid). Stigma receptivity was observed daily, from 7 days before anthesis until the anthesis stage. Furthermore, several types of pollination were evaluated, including open pollination, autogamy, geitonogamy, and allogamy. The results showed that the best staining method on P. cerasoides was aceto-orcein 2%, with pollen viability at 87.87%. The sucrose concentration of 25% at 72 hours of observation gave the highest pollen viability results, 52.48%. Stigma receptivity was optimal in the two days before anthesis until anthesis. The highest pollination efficiency was cross-pollination at 53.33%, with an average percentage of the total fruit set of 24.17%.
{"title":"Evaluation of reproduction biology of Prunus cerasoides","authors":"Suluh NORMASIWI, Siti S. HAFIZHAH, Ida ADVIANY, Muhammad I. SURYA","doi":"10.55779/nsb15311601","DOIUrl":"https://doi.org/10.55779/nsb15311601","url":null,"abstract":"Prunus cerasoides have a high value in phytochemistry and pharmacology. It was classified as the Least Concern globally based on the IUCN red list due to its widespread distribution in eastern Asia. This research aims to evaluate the reproductive biology of P. cerasoides through the study of pollen morphology, pollen viability, stigma receptivity, and pollination in the Cibodas Botanical Garden, located in the Cibodas subdistrict of West Java, Indonesia. The pollen morphology was observed using SEM. Moreover, the pollen viability test was followed by the staining method (aceto-orcein 2%, I2KI 1%, TTC 1%) and in vitro pollen germination with thirteen treatments (aquadest [control]; 5-30% sucrose; and 5-30% sucrose + 5 ppm boric acid). Stigma receptivity was observed daily, from 7 days before anthesis until the anthesis stage. Furthermore, several types of pollination were evaluated, including open pollination, autogamy, geitonogamy, and allogamy. The results showed that the best staining method on P. cerasoides was aceto-orcein 2%, with pollen viability at 87.87%. The sucrose concentration of 25% at 72 hours of observation gave the highest pollen viability results, 52.48%. Stigma receptivity was optimal in the two days before anthesis until anthesis. The highest pollination efficiency was cross-pollination at 53.33%, with an average percentage of the total fruit set of 24.17%.","PeriodicalId":19353,"journal":{"name":"Notulae Scientia Biologicae","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135097086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yaşar ÖZYİĞİT, Esra UÇAR, Nuraniye ERUYGUR, Mehmet ATAŞ, Merve İNANIR, Halil BAL, Danial KAHRIZI, Kenan TURGUT
This study examined the effects of different drying methods on the chemical content, antioxidant, antibacterial, enzymatic and anticancer activity values of stevia (Stevia rebaudiana Bert.) extract. Stevia leaves were dried using four different methods (in the sun, shade, air conditioner, and oven), and extracts were collected and analyzed. Based on extract content, 2-tetradecyl acrylate was the major ingredient in air-conditioned and oven-dried applications (25.00% and 21.47%, respectively). The same compound was detected in both sun-drying and shade-drying methods, but the content was low. The antioxidant activity values of the samples were evaluated with 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging tests. While the best result for DPPH (IC50 value 57.94 ± 0.63 µg/mL) was found in the shade-dried stevia samples, the best result in terms of ABTS test (IC50 value 44.03 ± 1.22 µg/mL) ml) was detected in oven-dried. When the samples were examined in terms of antimicrobial activity, it was seen that extracts of all drying methods were effective against Staphylococcus aureus. However, it was determined that the extracts obtained from plants dried in air conditioning and oven had a stronger effect. The cell viability assay was utilized to assess the antiproliferative effects of extract on L929 and MDA-MB-231 cell lines. The extracts did not significantly affect the L929 cell viability, while MDA-MB-231 remarkably reduced cell viability (sun drying, IC50 = 0.9 mg/mL; oven drying, IC50 = 0.65 mg/mL; shade drying, IC50 = 0.74 mg/mL; air conditioner drying, IC50 = 0.47 mg/mL). In particular, the extract obtained by the air conditioner drying method showed the most prominent cytotoxic effect. The results showed that drying using different methods had an impact on the quality standards of the stevia leaves.
{"title":"Comparison of different drying methods for phytochemical quality of stevia (Stevia rebaudiana Bert.)","authors":"Yaşar ÖZYİĞİT, Esra UÇAR, Nuraniye ERUYGUR, Mehmet ATAŞ, Merve İNANIR, Halil BAL, Danial KAHRIZI, Kenan TURGUT","doi":"10.55779/nsb15311527","DOIUrl":"https://doi.org/10.55779/nsb15311527","url":null,"abstract":"This study examined the effects of different drying methods on the chemical content, antioxidant, antibacterial, enzymatic and anticancer activity values of stevia (Stevia rebaudiana Bert.) extract. Stevia leaves were dried using four different methods (in the sun, shade, air conditioner, and oven), and extracts were collected and analyzed. Based on extract content, 2-tetradecyl acrylate was the major ingredient in air-conditioned and oven-dried applications (25.00% and 21.47%, respectively). The same compound was detected in both sun-drying and shade-drying methods, but the content was low. The antioxidant activity values of the samples were evaluated with 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging tests. While the best result for DPPH (IC50 value 57.94 ± 0.63 µg/mL) was found in the shade-dried stevia samples, the best result in terms of ABTS test (IC50 value 44.03 ± 1.22 µg/mL) ml) was detected in oven-dried. When the samples were examined in terms of antimicrobial activity, it was seen that extracts of all drying methods were effective against Staphylococcus aureus. However, it was determined that the extracts obtained from plants dried in air conditioning and oven had a stronger effect. The cell viability assay was utilized to assess the antiproliferative effects of extract on L929 and MDA-MB-231 cell lines. The extracts did not significantly affect the L929 cell viability, while MDA-MB-231 remarkably reduced cell viability (sun drying, IC50 = 0.9 mg/mL; oven drying, IC50 = 0.65 mg/mL; shade drying, IC50 = 0.74 mg/mL; air conditioner drying, IC50 = 0.47 mg/mL). In particular, the extract obtained by the air conditioner drying method showed the most prominent cytotoxic effect. The results showed that drying using different methods had an impact on the quality standards of the stevia leaves.","PeriodicalId":19353,"journal":{"name":"Notulae Scientia Biologicae","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135097088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
María E. GONZÁLEZ-VEGA, Merardo FERRER-VIVA, Yanelis CASTILLA-VALDÉS, Sandra PÉREZ-ÁLVAREZ, Esteban SÁNCHEZ, Víctor H. VILLARREAL-RAMÍREZ
The employment of biotechnological methods in conservation and multiplication of promissory genotypes of coffee is significant, but one of the limitations is the management of vitroplants during the adaptation phase. The aim of this research was to analyse the response of an F1 hybrid seedlings of coffee to the acclimatization phase with the application of new biofertilizers, respectively Pectimorf® and Viyoya®. As plant material seedlings of hybrid F1 H-434 obtained by somatic embryogenesis were used. The effect of Pectimorf® was determined in interaction with Rhizophagus intraradices and Funneliformis mosseae. In the second experiment, the growth and development of coffee seedlings was evaluated, with a biofertilizer based on efficient microorganisms (Viyoya®) combined with arbuscular mycorrhizal fungi. The study was carried out under greenhouse temperature of 25 °C and relative humidity of 75%. The results showed positive responses to the acclimatization of the seedlings with the bioproducts, in nutrition and growth. Concentrations of 10-20 mg L-1 of Pectimorf® guaranteed significant average increases for height, stem diameter and pairs of leaves, in combination with the R. intraradices strain (T3, T5). In addition, it had been seen that a favourable percentage of infestation was obtained in the soil used. The treatments with 1 and 3 mL of Viyoya® in combination with the AMF strains showed an increase in the indicators of plant height, leaf pairs and stem diameter, as well as the leaf area, the values being higher when applying 3 mL of Viyoya® (T3, T4, T5, T6). These results demonstrate the usefulness of plant growth promoting products in the acclimatization phase of an F1 hybrid seedlings of coffee.
{"title":"Response to the application of Pectimorf® and Viyoya® to coffee tree seedlings regenerated from germplasm conserved in vitro during the acclimatization phase","authors":"María E. GONZÁLEZ-VEGA, Merardo FERRER-VIVA, Yanelis CASTILLA-VALDÉS, Sandra PÉREZ-ÁLVAREZ, Esteban SÁNCHEZ, Víctor H. VILLARREAL-RAMÍREZ","doi":"10.55779/nsb15311518","DOIUrl":"https://doi.org/10.55779/nsb15311518","url":null,"abstract":"The employment of biotechnological methods in conservation and multiplication of promissory genotypes of coffee is significant, but one of the limitations is the management of vitroplants during the adaptation phase. The aim of this research was to analyse the response of an F1 hybrid seedlings of coffee to the acclimatization phase with the application of new biofertilizers, respectively Pectimorf® and Viyoya®. As plant material seedlings of hybrid F1 H-434 obtained by somatic embryogenesis were used. The effect of Pectimorf® was determined in interaction with Rhizophagus intraradices and Funneliformis mosseae. In the second experiment, the growth and development of coffee seedlings was evaluated, with a biofertilizer based on efficient microorganisms (Viyoya®) combined with arbuscular mycorrhizal fungi. The study was carried out under greenhouse temperature of 25 °C and relative humidity of 75%. The results showed positive responses to the acclimatization of the seedlings with the bioproducts, in nutrition and growth. Concentrations of 10-20 mg L-1 of Pectimorf® guaranteed significant average increases for height, stem diameter and pairs of leaves, in combination with the R. intraradices strain (T3, T5). In addition, it had been seen that a favourable percentage of infestation was obtained in the soil used. The treatments with 1 and 3 mL of Viyoya® in combination with the AMF strains showed an increase in the indicators of plant height, leaf pairs and stem diameter, as well as the leaf area, the values being higher when applying 3 mL of Viyoya® (T3, T4, T5, T6). These results demonstrate the usefulness of plant growth promoting products in the acclimatization phase of an F1 hybrid seedlings of coffee.","PeriodicalId":19353,"journal":{"name":"Notulae Scientia Biologicae","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135453439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chinedum U. EMELIKE, Ojichukwuka E. CHIJIOKE-AGU, Favour-Ann K. NWOKE, Romanus OYIBE, Chinwendu G. ENWEREUZO, Eze F. AHUEKWE
Diabetes mellitus is a metabolic dysfunction of insulin secretion exhibiting hyperglycemia with abnormalities in protein, fat and carbohydrate metabolism. The present study aimed to investigate the effect of Combretum dolichopetalum (CD) on hepatic parameters, renal indices and antioxidant markers of alloxan-induced diabetic rats. Twenty (20) male rats were used, and they were divided into four groups of five rats each. Group 1- Normal control received distilled water (non-diabetic control); Group 2- Diabetic control received distilled water; Group 3 and Group 4 are Diabetic rats treated with 200 mg/kg and 400 mg/kg of CD extract. There were significant decreases in the final body weights of Group 2 compared with Group 1 and an elevation of the body weights of Groups 3 and 4 compared with Group 2. There were significant increases in the blood glucose concentration of Group 2 compared with Group 1 and a reduction in blood glucose concentration of Groups 3 and 4 compared with Group 2. There were increases in the alanine amino transaminase (ALT), alkaline phosphatase (ALP) activities and serum total and conjugated bilirubin levels of Groups 2 and 3 compared with Group 1, but a reduction in the ALP, ALT activities and total bilirubin in Group 4. There was an elevation in the superoxide dismutase (SOD) the activity of Group 4. There were reductions in catalase activities and no change in the renal indices. The study showed ameliorating potentials on hyperglycemia, hepatoprotective activity and antioxidation effects.
{"title":"Modulation of antioxidant activities, markers of hepatic and renal dysfunctions in alloxan-induced diabetic rats by Combretum dolichopetalum","authors":"Chinedum U. EMELIKE, Ojichukwuka E. CHIJIOKE-AGU, Favour-Ann K. NWOKE, Romanus OYIBE, Chinwendu G. ENWEREUZO, Eze F. AHUEKWE","doi":"10.55779/nsb15311315","DOIUrl":"https://doi.org/10.55779/nsb15311315","url":null,"abstract":"Diabetes mellitus is a metabolic dysfunction of insulin secretion exhibiting hyperglycemia with abnormalities in protein, fat and carbohydrate metabolism. The present study aimed to investigate the effect of Combretum dolichopetalum (CD) on hepatic parameters, renal indices and antioxidant markers of alloxan-induced diabetic rats. Twenty (20) male rats were used, and they were divided into four groups of five rats each. Group 1- Normal control received distilled water (non-diabetic control); Group 2- Diabetic control received distilled water; Group 3 and Group 4 are Diabetic rats treated with 200 mg/kg and 400 mg/kg of CD extract. There were significant decreases in the final body weights of Group 2 compared with Group 1 and an elevation of the body weights of Groups 3 and 4 compared with Group 2. There were significant increases in the blood glucose concentration of Group 2 compared with Group 1 and a reduction in blood glucose concentration of Groups 3 and 4 compared with Group 2. There were increases in the alanine amino transaminase (ALT), alkaline phosphatase (ALP) activities and serum total and conjugated bilirubin levels of Groups 2 and 3 compared with Group 1, but a reduction in the ALP, ALT activities and total bilirubin in Group 4. There was an elevation in the superoxide dismutase (SOD) the activity of Group 4. There were reductions in catalase activities and no change in the renal indices. The study showed ameliorating potentials on hyperglycemia, hepatoprotective activity and antioxidation effects.","PeriodicalId":19353,"journal":{"name":"Notulae Scientia Biologicae","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135453440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Maazouzi, Jalila Aoujdad, K. Selmaoui, M. Chliyeh, N. Mouden, S. Msairi, S. Elantry, Mustapha Azeroual, Mohamed Ouajdi, Mohamed Karimi, A. Ouazzani Touhami, A. Douira
The argan tree, an endemic species in Morocco, has been exhaustively studied in its southwestern range but neglected in its northeastern territory. Thus, the present study sets as objectives the identification of arbuscular mycorrhizal fungi and the evaluation of the argan tree’s roots’ mycorrhization level in two stations: one in the region of Béni Snassène (Berkane): Jebel Takermine with 7 prospected sites and the other at Jebel Aklim Alkabir with 2 sites. At the first station (Jebel Takermine), the mycorrhizal frequency of Argania spinosa roots varied between 64% and 100% with the root mycorrhizal intensity in the range of 30.77% and 66%. The arbuscular contents are higher at sites 2 (50.46%), 4 (50.33%), 7 (39.44%) and 1 (30.5%) against 18% and 20% at sites 6, 5 and 3. Argan trees from Jbel Aklim Alkbir, exhibited a high mycorrhization frequency and intensity ranging from 88% to 100% and between 39.4% and 73.4% respectively. Regarding arbuscular and vesicular rates, the highest values were associated to the roots of site 1 with 59.3% and 29.4% respectively compared to the lowest rates of 20% and 14% in those of site 2. Spore densities in the rhizosphere of the studied argan trees in the two stations were in the range of 78 and 697 spores/100 g of soil. The identification of isolated mycorrhizal spores revealed the presence of 28 species encompassing 7 genera: Acaulospora, Dentiscutata Claroideoglomus, Funneliformis, Glomus, Rhizophagus, Pacispora, and 5 Families: Glomeraceae (7 species), Acaulosporaceae (10 species), Pacisporaceae (2 species), Claroideoglomeraceae (2 species), Gigasporaceae (1 species).
{"title":"Mycorrhizal status of Argania spinosa (L.) Skeels in northeastern of Morocco","authors":"S. Maazouzi, Jalila Aoujdad, K. Selmaoui, M. Chliyeh, N. Mouden, S. Msairi, S. Elantry, Mustapha Azeroual, Mohamed Ouajdi, Mohamed Karimi, A. Ouazzani Touhami, A. Douira","doi":"10.55779/nsb15211405","DOIUrl":"https://doi.org/10.55779/nsb15211405","url":null,"abstract":" The argan tree, an endemic species in Morocco, has been exhaustively studied in its southwestern range but neglected in its northeastern territory. Thus, the present study sets as objectives the identification of arbuscular mycorrhizal fungi and the evaluation of the argan tree’s roots’ mycorrhization level in two stations: one in the region of Béni Snassène (Berkane): Jebel Takermine with 7 prospected sites and the other at Jebel Aklim Alkabir with 2 sites. At the first station (Jebel Takermine), the mycorrhizal frequency of Argania spinosa roots varied between 64% and 100% with the root mycorrhizal intensity in the range of 30.77% and 66%. The arbuscular contents are higher at sites 2 (50.46%), 4 (50.33%), 7 (39.44%) and 1 (30.5%) against 18% and 20% at sites 6, 5 and 3. Argan trees from Jbel Aklim Alkbir, exhibited a high mycorrhization frequency and intensity ranging from 88% to 100% and between 39.4% and 73.4% respectively. Regarding arbuscular and vesicular rates, the highest values were associated to the roots of site 1 with 59.3% and 29.4% respectively compared to the lowest rates of 20% and 14% in those of site 2. Spore densities in the rhizosphere of the studied argan trees in the two stations were in the range of 78 and 697 spores/100 g of soil. The identification of isolated mycorrhizal spores revealed the presence of 28 species encompassing 7 genera: Acaulospora, Dentiscutata Claroideoglomus, Funneliformis, Glomus, Rhizophagus, Pacispora, and 5 Families: Glomeraceae (7 species), Acaulosporaceae (10 species), Pacisporaceae (2 species), Claroideoglomeraceae (2 species), Gigasporaceae (1 species).","PeriodicalId":19353,"journal":{"name":"Notulae Scientia Biologicae","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43934273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Notulae Scientia Biologicae (http://www.notulaebiologicae.ro), Issue 2, Volume 15, 2023: The papers published in this issue represent interesting novelties in different topics of life science. Among the exciting research or reviews, we invite readers to find news about: Neuroprotective role of a protoberberine alkaloid against aluminum-induced neuroinflammation and excitotoxicity; Evaluation of cross-genus transferability of SSR markers from other legumes to two closely related Onobrychis (Fabaceae) taxa; Morphological characterization and cytological studies of the green-stemmed and the red-stemmed Basella alba; Wickerhamomyces anomalus WO2 inoculation protects Suaeda fruticosa from salt stress by the stimulation of the production of carotenoids and sugars and the augmentation of sodium translocation; Mycorrhizal status of Argania spinosa (L.) Skeels in northeastern of Morocco; Evaluation of two sampling methods for even-age Pinus forest in northern Mexico.
《Notulae Scientia Biologicae》(http://www.notulaebiologicae.ro)，第2期，第15卷，2023年:这期发表的论文代表了生命科学不同主题的有趣的新奇事物。在这些令人兴奋的研究或评论中，我们邀请读者发现以下新闻:原小檗碱生物碱对铝诱导的神经炎症和兴奋性毒性的神经保护作用;其他豆科植物SSR标记在两个近缘豆科单藓属分类群间的可转移性评价绿茎和红茎白葵的形态特征和细胞学研究接种异常柳杉WO2通过刺激类胡萝卜素和糖的产生和增加钠的转运来保护盐胁迫下的Suaeda frutisa;麻蚶菌根状况研究摩洛哥东北部的骨骸;墨西哥北部等龄松林两种取样方法的评价。
{"title":"Introduction pages, Notulae Scientia Biologicae 15(2), 2023","authors":"Radu E. Sestras","doi":"10.55779/nsb15211611","DOIUrl":"https://doi.org/10.55779/nsb15211611","url":null,"abstract":"Notulae Scientia Biologicae (http://www.notulaebiologicae.ro), Issue 2, Volume 15, 2023: The papers published in this issue represent interesting novelties in different topics of life science. Among the exciting research or reviews, we invite readers to find news about: Neuroprotective role of a protoberberine alkaloid against aluminum-induced neuroinflammation and excitotoxicity; Evaluation of cross-genus transferability of SSR markers from other legumes to two closely related Onobrychis (Fabaceae) taxa; Morphological characterization and cytological studies of the green-stemmed and the red-stemmed Basella alba; Wickerhamomyces anomalus WO2 inoculation protects Suaeda fruticosa from salt stress by the stimulation of the production of carotenoids and sugars and the augmentation of sodium translocation; Mycorrhizal status of Argania spinosa (L.) Skeels in northeastern of Morocco; Evaluation of two sampling methods for even-age Pinus forest in northern Mexico.","PeriodicalId":19353,"journal":{"name":"Notulae Scientia Biologicae","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135050262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ping Wang, Lingjuan Man, Li Ma, Jiaxin Qi, Yanping Ren, Z. Yao, Bo Wang, Cong Cheng, Hua Zhang
Haloxylon ammodendron (C.A.Mey) Bunge is one of the important species of arid desert vegetation in China, and it is also an ideal material for studying plant stress resistance, which plays an extremely important role in protecting desert ecosystems and preventing land desertification. However, there are relatively few studies on the regeneration of the fusiform, and the genetic transformation system has not been successfully reported, which restricts the in-depth study of the molecular mechanism of shuttle propagation and stress resistance. In the present study, the seeds, hypocotyls, cotyledons, cotyledon nodes, terminal buds and fixed buds were used as explants, and a set of tissue culture and plant regeneration system was established by inducing adventitious buds, adventitious bud rooting and transplanting. The results showed that amongst different H. ammodendron explants, i.e., seeds, hypocotyls, cotyledons and cotyledon nodes, the last induced budding effect was better. The optimal medium for inducing clandine buds by cotyledon segment differentiation is 0.5 mg·L-1 NAA+0.5 mg·L-1 6-BA induced budding rate was high, reaching 61.90%, rooting medium was 1/2 MS+1 mg·L-1 NAA +1 mg·L-1 IBA + 1 mg·L-1 IAA with a rooting rate of 50%. The results of this study will provide a theoretical basis for the genetic transformation of H. ammodendron.
{"title":"In vitro regeneration of Haloxylon ammodendron","authors":"Ping Wang, Lingjuan Man, Li Ma, Jiaxin Qi, Yanping Ren, Z. Yao, Bo Wang, Cong Cheng, Hua Zhang","doi":"10.55779/nsb15211585","DOIUrl":"https://doi.org/10.55779/nsb15211585","url":null,"abstract":"Haloxylon ammodendron (C.A.Mey) Bunge is one of the important species of arid desert vegetation in China, and it is also an ideal material for studying plant stress resistance, which plays an extremely important role in protecting desert ecosystems and preventing land desertification. However, there are relatively few studies on the regeneration of the fusiform, and the genetic transformation system has not been successfully reported, which restricts the in-depth study of the molecular mechanism of shuttle propagation and stress resistance. In the present study, the seeds, hypocotyls, cotyledons, cotyledon nodes, terminal buds and fixed buds were used as explants, and a set of tissue culture and plant regeneration system was established by inducing adventitious buds, adventitious bud rooting and transplanting. The results showed that amongst different H. ammodendron explants, i.e., seeds, hypocotyls, cotyledons and cotyledon nodes, the last induced budding effect was better. The optimal medium for inducing clandine buds by cotyledon segment differentiation is 0.5 mg·L-1 NAA+0.5 mg·L-1 6-BA induced budding rate was high, reaching 61.90%, rooting medium was 1/2 MS+1 mg·L-1 NAA +1 mg·L-1 IBA + 1 mg·L-1 IAA with a rooting rate of 50%. The results of this study will provide a theoretical basis for the genetic transformation of H. ammodendron.","PeriodicalId":19353,"journal":{"name":"Notulae Scientia Biologicae","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43922099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Viridiana S. Galván-Moreno, J. M. Olivas-García, Joel Rascón-Solano, J. Hernández-Salas
The objective was to evaluate and compare the precision of estimation of dasometric variables, survey times and costs of two sampling methods versus a census, applied to a natural even-age mass of Pinus arizonica Engelm. in the municipality of Guachochi, Chihuahua, Mexico. Nine fixed-dimension and nine variable-dimension sites were performed with Bitterlich's angular sampling at coincident sample points. Times from start to finish were taken by site and technicians from the State were surveyed to determine sampling costs. ANOVA tests were developed at a significance level of 0.05, comparing both methods with the census. The results indicate that, in both samplings, the number of trees per hectare does not present statistical differences with respect to the census. The basal area by both methods was not statistically different, however, fixed dimensions present an error greater than 5%. Finally, the volume per hectare estimated was similar to that of the census, according to the rates carried out, greater precision was found by the variable area method (error=0.03%). The execution time showed significant differences (p=0.0001), the fixed dimension site required a mean time of 44 minutes and the variable dimension one 10 minutes 26 seconds. The total costs are 679.90 and 654.33 Euro to sample nine fixed-dimension and nine variable-dimension sites respectively, showing significant differences (p=0.0008). It is concluded that both methods are statistically acceptable for the variables evaluated in the type of forest mass studied.
{"title":"Evaluation of two sampling methods for even-age Pinus forest in northern Mexico","authors":"Viridiana S. Galván-Moreno, J. M. Olivas-García, Joel Rascón-Solano, J. Hernández-Salas","doi":"10.55779/nsb15211564","DOIUrl":"https://doi.org/10.55779/nsb15211564","url":null,"abstract":"The objective was to evaluate and compare the precision of estimation of dasometric variables, survey times and costs of two sampling methods versus a census, applied to a natural even-age mass of Pinus arizonica Engelm. in the municipality of Guachochi, Chihuahua, Mexico. Nine fixed-dimension and nine variable-dimension sites were performed with Bitterlich's angular sampling at coincident sample points. Times from start to finish were taken by site and technicians from the State were surveyed to determine sampling costs. ANOVA tests were developed at a significance level of 0.05, comparing both methods with the census. The results indicate that, in both samplings, the number of trees per hectare does not present statistical differences with respect to the census. The basal area by both methods was not statistically different, however, fixed dimensions present an error greater than 5%. Finally, the volume per hectare estimated was similar to that of the census, according to the rates carried out, greater precision was found by the variable area method (error=0.03%). The execution time showed significant differences (p=0.0001), the fixed dimension site required a mean time of 44 minutes and the variable dimension one 10 minutes 26 seconds. The total costs are 679.90 and 654.33 Euro to sample nine fixed-dimension and nine variable-dimension sites respectively, showing significant differences (p=0.0008). It is concluded that both methods are statistically acceptable for the variables evaluated in the type of forest mass studied.","PeriodicalId":19353,"journal":{"name":"Notulae Scientia Biologicae","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46478775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of the research was to determine the influence of varietal characteristics, pre-sowing seed treatment and irrigation on the yield of winter wheat (Triticum aestivum L.). Experimental studies were carried out in the conditions of Mykolayiv National Agrarian University (Ukraine) during 2019-2022 yrs. It was found that among the studied variety of winter wheat, the highest grain yield was distinguished by the variety ‘Ozerna’ and ‘Duma Odes’ka’. On average, for pre – sowing seed treatment and irrigation options, these variety formed yields at the level of 6.78 and 6.95 tha–1, which was higher than the grain yield of ‘Anatoliya’ variety by 4.1 up to 6.5%, and then the yield of ‘Ovidii’ variety by 3.9 up to 6.3%. It was proved that preparations for pre-sowing seed treatment contributed to the formation of more productive stems and grain mass from one ear in winter wheat plants. So, on average, over the years of research, variety and irrigation options, for the use of Azotofit-R, the number of productive stems increased by 34-74 PCs./ m2 compared to other seed treatment options. At the same time, the yield of winter wheat grain was also slightly higher with the use of Azotofit-R biologics. It was found that the use of irrigation provided an increase in grain yield by 1.66-2.19 tha–1 or 23.1-27.2%, depending on the studied winter wheat variety. At the same time, the ‘Duma Odes’ka’ variety had a higher grain yield under irrigation (8.06 tha–1), while in conditions without irrigation, a slightly higher grain yield was formed by plants of the ‘Ozerna’ variety (5.87 tha–1). On average, over the years of research, the highest yield of winter wheat grain was provided by the cultivation of the ‘Duma Odes’ka’ variety for pre-sowing seed treatment with Azotofit - R biologics and irrigation–8.38 tha–1.
{"title":"Influence of biologics on the productivity of winter wheat varieties under irrigation conditions","authors":"A. Panfilova, M. Korkhova, N. Markova","doi":"10.55779/nsb15211352","DOIUrl":"https://doi.org/10.55779/nsb15211352","url":null,"abstract":"The aim of the research was to determine the influence of varietal characteristics, pre-sowing seed treatment and irrigation on the yield of winter wheat (Triticum aestivum L.). Experimental studies were carried out in the conditions of Mykolayiv National Agrarian University (Ukraine) during 2019-2022 yrs. It was found that among the studied variety of winter wheat, the highest grain yield was distinguished by the variety ‘Ozerna’ and ‘Duma Odes’ka’. On average, for pre – sowing seed treatment and irrigation options, these variety formed yields at the level of 6.78 and 6.95 tha–1, which was higher than the grain yield of ‘Anatoliya’ variety by 4.1 up to 6.5%, and then the yield of ‘Ovidii’ variety by 3.9 up to 6.3%. It was proved that preparations for pre-sowing seed treatment contributed to the formation of more productive stems and grain mass from one ear in winter wheat plants. So, on average, over the years of research, variety and irrigation options, for the use of Azotofit-R, the number of productive stems increased by 34-74 PCs./ m2 compared to other seed treatment options. At the same time, the yield of winter wheat grain was also slightly higher with the use of Azotofit-R biologics. It was found that the use of irrigation provided an increase in grain yield by 1.66-2.19 tha–1 or 23.1-27.2%, depending on the studied winter wheat variety. At the same time, the ‘Duma Odes’ka’ variety had a higher grain yield under irrigation (8.06 tha–1), while in conditions without irrigation, a slightly higher grain yield was formed by plants of the ‘Ozerna’ variety (5.87 tha–1). On average, over the years of research, the highest yield of winter wheat grain was provided by the cultivation of the ‘Duma Odes’ka’ variety for pre-sowing seed treatment with Azotofit - R biologics and irrigation–8.38 tha–1.","PeriodicalId":19353,"journal":{"name":"Notulae Scientia Biologicae","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48192820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Enterococcus faecium is emerging as an important cause of multidrug resistance and hospital acquired infections, special attention being paid to the vancomycin resistant species. Therefore, the characterization of pathogenic strains/isolates plays an important role in the epidemiology of infectious diseases. The enterococcal rate was determined from wastewaters in Cluj-Napoca area. As presence of E. faecium was detected, a number of isolates from wastewater, birds and humans were epidemiologically analyzed according to the MLST website. Comparisons were performed against a collection of available isolates, with multiple origins, contained in the MLST database. Out of the Enterococcus isolates collected from wastewater, 11 were identified as E. faecalis (40.74%); 8 as E. casseliflavus (29.62%); 5 as E. faecium (18.50%); 2 as E. gallinarum (7.40%) and one isolate as E. durans. Based on the MLST data and using the eBURST algorithm, the isolates of E. faecium sampled from Romania were split in three groups: one group comprised isolates from human hosts and wastewater (Cj316, 106/6, Cj197, Cj22, 129/6, Cj117, Cj24, 284/7, and 43/7), while the second (G9, G10-2, G7, G3-2, and G9-1) and the third group (G8, G6, and 40/7) originated from bird hosts. The rest of the isolates were not joined in a particular group, assuming the lack of a phylogenetic bond between these isolates. The obtained data suggested the existence of at least two phylogenetic lines of E. faecium in Romania: a line that had mainly human host prevalence, while in the other line the animal hosts dominated.
{"title":"Epidemiology of Enterococcus faecium isolates sampled from different sources in Romania using MLST technique and eBURST algorithm","authors":"I. Băcilă, E. Jakab, Dana Șuteu, O. Popescu","doi":"10.55779/nsb15211546","DOIUrl":"https://doi.org/10.55779/nsb15211546","url":null,"abstract":"Enterococcus faecium is emerging as an important cause of multidrug resistance and hospital acquired infections, special attention being paid to the vancomycin resistant species. Therefore, the characterization of pathogenic strains/isolates plays an important role in the epidemiology of infectious diseases. The enterococcal rate was determined from wastewaters in Cluj-Napoca area. As presence of E. faecium was detected, a number of isolates from wastewater, birds and humans were epidemiologically analyzed according to the MLST website. Comparisons were performed against a collection of available isolates, with multiple origins, contained in the MLST database. Out of the Enterococcus isolates collected from wastewater, 11 were identified as E. faecalis (40.74%); 8 as E. casseliflavus (29.62%); 5 as E. faecium (18.50%); 2 as E. gallinarum (7.40%) and one isolate as E. durans. Based on the MLST data and using the eBURST algorithm, the isolates of E. faecium sampled from Romania were split in three groups: one group comprised isolates from human hosts and wastewater (Cj316, 106/6, Cj197, Cj22, 129/6, Cj117, Cj24, 284/7, and 43/7), while the second (G9, G10-2, G7, G3-2, and G9-1) and the third group (G8, G6, and 40/7) originated from bird hosts. The rest of the isolates were not joined in a particular group, assuming the lack of a phylogenetic bond between these isolates. The obtained data suggested the existence of at least two phylogenetic lines of E. faecium in Romania: a line that had mainly human host prevalence, while in the other line the animal hosts dominated. ","PeriodicalId":19353,"journal":{"name":"Notulae Scientia Biologicae","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45543943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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