Nuclear medicine and biology最新文献

{"title":"Modular molecular and nuclear design of scaffold protein -mediated PNA-based radionuclide pre-targeting: application for DARPins","authors":"Vladimir Tolmachev , Eleftherios Papalanis , Zhengyue Wang , Ivan Zelepukin , Yongsheng Liu , Anna Orlova , Kristina Westerlund , Amelie Eriksson Karlström , Maryam Oroujeni","doi":"10.1016/j.nucmedbio.2025.109162","DOIUrl":"10.1016/j.nucmedbio.2025.109162","url":null,"abstract":"","PeriodicalId":19363,"journal":{"name":"Nuclear medicine and biology","volume":"150 ","pages":"Article 109162"},"PeriodicalIF":3.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145473739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corticolimbic activation patterns during aversive audiogenic stress assessed by cerebral perfusion using [99mTc]HMPAO SPECT/CT","authors":"Angel Moldes-Anaya ,&nbsp;Lorenzo Ragazzi ,&nbsp;Torgil R. Vangberg ,&nbsp;Samuel Kuttner ,&nbsp;Vegard H. Brun ,&nbsp;Kirsten B. Kjelstrup","doi":"10.1016/j.nucmedbio.2025.109562","DOIUrl":"10.1016/j.nucmedbio.2025.109562","url":null,"abstract":"<div><h3>Background</h3><div>Emotional experiences modulate behavior by influencing the central and peripheral nervous systems, playing a crucial role in the pathophysiology of neuropsychiatric disorders. This study investigates the neural mechanisms underlying anxiety-like responses induced by an aversive acoustic stimulus in Wistar rats. Using [^99mTc]HMPAO single photon emission computed tomography / computed tomography (SPECT/CT) imaging, we assessed regional cerebral blood flow (rCBF) changes and correlated them with behavioral responses.</div></div><div><h3>Methods</h3><div>Male rats were subjected to either an unpredictable acoustic stressor or control conditions in an open field paradigm (OFP). Behavioral assessments included freezing, grooming, rearing, and escape responses, with blood samples collected to measure change in corticosterone levels. SPECT/CT imaging was performed after both stimulation and retention sessions to analyze changes in rCBF.</div></div><div><h3>Results</h3><div>Our results demonstrate significant increased brain activity of the ventral hippocampus, amygdala, periaqueductal gray, hypothalamus and entorhinal/perirhinal areas with engagement of retrosplenial, frontal association and auditory cortices during the presentation of the acoustic aversive stimulus. Additionally, decreased brain activity of the orbitofrontal cortex, ventral striatum, basal forebrain and motor/somatosensory and cerebellar clusters are also observed, all consistent with a rapidly assembled defensive state integrated across the uptake window. Behaviorally, stressed animals exhibited escape responses as well as increased freezing and reduced grooming and exploratory rearing during exposure, indicating heightened anxiety. Upon context re-exposure, we observed lower activation patterns in retrosplenial cortex and dorsal hippocampus and higher activity in cingulate and orbitofrontal cortices, with small right-lateral amygdala/bed nucleus of stria terminalis clusters. Zona incerta, hypothalamus, piriform cortex and basal forebrain showed relative increased activation, while periaqueductal gray and inferior colliculus activation were reduced, indicating early-stage monitoring and vigilance with limited context-map engagement, rather than robust context fear retrieval. Corticosterone levels rose from pre- to post-session in both groups without intergroup differences.</div></div><div><h3>Conclusion</h3><div>Time-anchored perfusion SPECT delineates a threat-responsive mesocorticolimbic-midbrain circuitry during unexpected acoustic stress, prominently implicating the ventral hippocampus, and reveals a shift at context re-entry toward prefrontal monitoring with attenuated hippocampal/retrosplenial and midbrain defensive signals. These findings contribute to understanding the neural substrates of anxiety disorders, with implications for developing targeted interventions.</div></div>","PeriodicalId":19363,"journal":{"name":"Nuclear medicine and biology","volume":"150 ","pages":"Article 109562"},"PeriodicalIF":3.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145346252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative Flash Deprotection in Large-Scale Production of [131I]I-SGMIB labeled single-domain antibody targeting HER2","authors":"Laurent Navarro,&nbsp;Nicolas Verbrugge,&nbsp;Jos Eersels,&nbsp;Vaiva Gaspariunaite,&nbsp;Aleksandra Kotwicka,&nbsp;Matthias Friebe,&nbsp;Dimitrios Mantzilas,&nbsp;Matthias D’Huyvetter","doi":"10.1016/j.nucmedbio.2025.109157","DOIUrl":"10.1016/j.nucmedbio.2025.109157","url":null,"abstract":"","PeriodicalId":19363,"journal":{"name":"Nuclear medicine and biology","volume":"150 ","pages":"Article 109157"},"PeriodicalIF":3.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145473834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and Preclinical Evaluation of GRPR Antagonist [18F]MeTz-PEG2-RM26 for Position Emission Tomography","authors":"Anna Orlova ,&nbsp;Panagiotis Kanellopoulos ,&nbsp;Fanny Lundmark ,&nbsp;Ayman Abouzayed ,&nbsp;Lorenzo Balestri ,&nbsp;Esther Olaniran Håkansson ,&nbsp;Karim Obeid ,&nbsp;Luke R. Odell ,&nbsp;Vladimir Tolmachev ,&nbsp;Ulrika Rosenström ,&nbsp;Jonas Eriksson","doi":"10.1016/j.nucmedbio.2025.109142","DOIUrl":"10.1016/j.nucmedbio.2025.109142","url":null,"abstract":"","PeriodicalId":19363,"journal":{"name":"Nuclear medicine and biology","volume":"150 ","pages":"Article 109142"},"PeriodicalIF":3.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145473959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Studies towards the copper-mediated radiofluorination of a nitrogen-containing anti-cancer drug candidate in the presence of ligands","authors":"Kevin Bajerke ,&nbsp;Kalman Szabo ,&nbsp;Gunnar Antoni ,&nbsp;Fredrik Lehmann","doi":"10.1016/j.nucmedbio.2025.109185","DOIUrl":"10.1016/j.nucmedbio.2025.109185","url":null,"abstract":"","PeriodicalId":19363,"journal":{"name":"Nuclear medicine and biology","volume":"150 ","pages":"Article 109185"},"PeriodicalIF":3.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145474168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radiolabelling bacteria with 89Zr-DBN for PET/MRI: comparing [89Zr]Zr oxalate and [89Zr]Zr phosphate","authors":"Diksha ,&nbsp;Sarah C. Donnelly ,&nbsp;Mojmir Suchy ,&nbsp;Neil Cockburn ,&nbsp;Frank S. Prato ,&nbsp;Michael S. Kovacs ,&nbsp;Donna E. Goldhawk","doi":"10.1016/j.nucmedbio.2025.109577","DOIUrl":"10.1016/j.nucmedbio.2025.109577","url":null,"abstract":"<div><h3>Purpose</h3><div>Our bodies host a diverse and complex community of bacteria, known as the microbiota, which play a crucial role in overall wellness. The gut microbiota perform essential functions, such as food fermentation, pathogen protection, and vitamin production. Dysbiosis, or the imbalance of these bacterial communities, can disrupt these processes, leading to diseases like cancer, respiratory infections, and neurological disorders. To enable non-invasive tracking of microbial distribution <em>in vivo</em>, this study investigates [⁸⁹Zr]Zr oxalate as a more efficient and cost-effective alternative to [⁸⁹Zr]Zr phosphate for radiolabelling diverse bacterial species for PET/MRI.</div></div><div><h3>Methods</h3><div>To test radiolabelling efficiency, the bifunctional chelator DBN, consisting of desferrioxamine (DFO) to chelate ⁸⁹Zr and a lysine-reactive group (isothiocyanate) for cell surface protein attachment, was used. The bifunctional chelator was mixed with either neutralized [⁸⁹Zr]Zr phosphate (titrated to pH 7 with 1 M K₂CO₃) in HEPES buffer or [⁸⁹Zr]Zr oxalate (titrated to pH 7 with 2 M Na₂CO₃) in phosphate buffered saline (PBS). The efficiency of isotope chelation was evaluated by radio thin layer chromatography (radio-TLC). The efficiency of bacterial conjugation was examined with commensal <em>Lactobacillus crispatus</em> ATCC33820 and probiotic <em>Escherichia coli</em> Nissle 1917. A <em>t</em>-test with unequal variances assessed statistical differences in chelation and bacterial labelling efficiencies.</div></div><div><h3>Results</h3><div>[⁸⁹Zr]Zr oxalate showed significantly higher chelation efficiency to DBN (94.8 ± 5.5 %) compared to [⁸⁹Zr]Zr phosphate (66.6 ± 16.3 %; <em>p</em> &lt; 0.05). Labelling efficiency for <em>L. crispatus</em> improved when [⁸⁹Zr]Zr oxalate was used either after one half-life had passed since production or within 1 half-life but diluted with water in a 1:1 ratio. Optimal labelling provided an average activity per live cell (colony-forming unit, CFU) between 0.003 and 0.12 Bq/CFU, with activities above 0.01 Bq/CFU causing significant bacterial death, while lower activities (below 0.006 Bq/CFU) effectively maintained bacterial viability. Comparable bacterial labelling efficiency results were obtained with <em>Escherichia coli</em> Nissle 1917.</div></div><div><h3>Conclusion</h3><div>This study establishes the first standardized protocol for radiolabelling viable bacteria using ⁸⁹Zr-DBN derived from [⁸⁹Zr]Zr oxalate. This method enables high chelation and labelling efficiency while preserving cell viability and is applicable to both <em>L. crispatus</em> (Gram-positive, facultative anaerobe) and <em>E.coli</em> (Gram-negative, aerobe), supporting its use in PET/MRI based bacterial imaging.</div></div>","PeriodicalId":19363,"journal":{"name":"Nuclear medicine and biology","volume":"150 ","pages":"Article 109577"},"PeriodicalIF":3.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145416267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of Aliphatic Sulfamoyl [18F]Fluorides ([18F]ASAFs) Using the \"Minimalist Green\" Approach","authors":"Jan Bertram ,&nbsp;Bernd Neumaier ,&nbsp;Boris Zlatopolskiy ,&nbsp;Christian Weisselberg","doi":"10.1016/j.nucmedbio.2025.109119","DOIUrl":"10.1016/j.nucmedbio.2025.109119","url":null,"abstract":"","PeriodicalId":19363,"journal":{"name":"Nuclear medicine and biology","volume":"150 ","pages":"Article 109119"},"PeriodicalIF":3.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145473330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Multimodal Metamaterial Contrast Agent for Cerenkov Luminescence Imaging Enhancement","authors":"Natalie Boykoff ,&nbsp;Samhita Kattekola ,&nbsp;Ilona Kretzschmar ,&nbsp;Jan Grimm ,&nbsp;Stephen O'Brien ,&nbsp;Vinod Menon","doi":"10.1016/j.nucmedbio.2025.109146","DOIUrl":"10.1016/j.nucmedbio.2025.109146","url":null,"abstract":"","PeriodicalId":19363,"journal":{"name":"Nuclear medicine and biology","volume":"150 ","pages":"Article 109146"},"PeriodicalIF":3.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145473566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selective CXCR4-Targeting Antagonists Derived from LY2510924: [¹⁸F]AlF-NOTA-SC, [¹⁷⁷Lu]Lu-BL02, and [¹⁶¹Tb]Tb-BL02 as a Promising Trio for Precision Imaging and Therapy","authors":"Muriel Spahn,&nbsp;Dominique Schols,&nbsp;Tom Van Loy,&nbsp;Sofie Celen,&nbsp;Michel Koole,&nbsp;Christophe Deroose,&nbsp;Christopher Cawthorne,&nbsp;Guy Bormans,&nbsp;Frederik Cleeren","doi":"10.1016/j.nucmedbio.2025.109099","DOIUrl":"10.1016/j.nucmedbio.2025.109099","url":null,"abstract":"","PeriodicalId":19363,"journal":{"name":"Nuclear medicine and biology","volume":"150 ","pages":"Article 109099"},"PeriodicalIF":3.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145473533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Umpolung C-H (Radio)fluorination of Hydroxamide Derivatives via Strained Aziridinone Intermediates Enabled by N-O Scission","authors":"Siran Qian ,&nbsp;Surendra Reddy Gundam ,&nbsp;Jay Wright","doi":"10.1016/j.nucmedbio.2025.109168","DOIUrl":"10.1016/j.nucmedbio.2025.109168","url":null,"abstract":"","PeriodicalId":19363,"journal":{"name":"Nuclear medicine and biology","volume":"150 ","pages":"Article 109168"},"PeriodicalIF":3.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145473648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}