Kawan Lama Group has been established since 1955 and until now has several business fields engaged in the Retail, Industrial, Food and Beverage, Service, Property and E-commerce sectors. For more than half a century, Kawan Lama continues to grow and develop to employ more than 40,000 employees. Kawan Lama Group's vision is more than just a family business, we are a business for families. Our mission is to bring values to a better life through business development and sustainable growth. PT. Home Center Kawan Lama or Informa is a furniture company starting from home furniture, office furniture, to commercial space furniture. Informa provides more than 35,000 quality items that can meet the needs of customers to have their dream furniture. The NASA-TLX method was developed by Sandra. G Hart from NASA-Ames Research Center and Lowell E. Staveland from San Jose State University in 1981. This method performs multidimensional measurements by considering weights and grades. The level of the first factor relates to the work performed, while the other three factors relate to the subject. The average WWL (weighted workload) mental workload of customer service employees prior to the proposed work shift improvement, application of music therapy and motivation is the morning shift of 76.78, the afternoon shift of 77.97 and the night shift of 80. Average Results WWL (weighted workload) mental workload of employees in the morning, afternoon and evening shifts shows a high mental workload. The morning, afternoon and evening shifts have significant differences in physical needs, mental needs, time requirements, performance, stress levels and average WWL (weighted workload).
卡旺喇嘛集团成立于1955年，目前拥有零售、工业、餐饮、服务、房地产和电子商务等多个业务领域。半个多世纪以来，卡旺喇嘛不断发展壮大，拥有超过40,000名员工。卡旺喇嘛集团的愿景不仅仅是一个家族企业，我们是一个为家庭服务的企业。我们的使命是通过业务发展和可持续增长为更美好的生活带来价值。PT. Home Center Kawan Lama或Informa是一家从家居家具，办公家具，到商业空间家具的家具公司。Informa提供超过35,000件优质产品，可以满足客户拥有梦想家具的需求。NASA-TLX方法是桑德拉开发的。NASA-Ames研究中心的G Hart和圣何塞州立大学的Lowell E. Staveland在1981年。该方法通过考虑权重和等级执行多维度量。第一个因素的水平与完成的工作有关，而其他三个因素与主题有关。在建议的工作班次改善、应用音乐疗法和激励之前，客服员工的平均WWL(加权工作量)心理工作量为早班76.78，下午77.97，夜班80。平均结果早、中、晚班员工的WWL(加权工作量)心理工作量表现出较高的心理工作量。早班、中班和晚班在生理需求、心理需求、时间要求、工作表现、压力水平和平均加权工作量方面存在显著差异。
{"title":"Mental Workload Analysis Using NASA-TLX Method on Customer Service Employees in Strategist Informa Social Media Division (PT Home Center Kawan Lama)","authors":"Ratih Permata Sari, Rini Setiowati, Anggi Oktaviani","doi":"10.37010/nuc.v3i1.671","DOIUrl":"https://doi.org/10.37010/nuc.v3i1.671","url":null,"abstract":"Kawan Lama Group has been established since 1955 and until now has several business fields engaged in the Retail, Industrial, Food and Beverage, Service, Property and E-commerce sectors. For more than half a century, Kawan Lama continues to grow and develop to employ more than 40,000 employees. Kawan Lama Group's vision is more than just a family business, we are a business for families. Our mission is to bring values to a better life through business development and sustainable growth. PT. Home Center Kawan Lama or Informa is a furniture company starting from home furniture, office furniture, to commercial space furniture. Informa provides more than 35,000 quality items that can meet the needs of customers to have their dream furniture. The NASA-TLX method was developed by Sandra. G Hart from NASA-Ames Research Center and Lowell E. Staveland from San Jose State University in 1981. This method performs multidimensional measurements by considering weights and grades. The level of the first factor relates to the work performed, while the other three factors relate to the subject. The average WWL (weighted workload) mental workload of customer service employees prior to the proposed work shift improvement, application of music therapy and motivation is the morning shift of 76.78, the afternoon shift of 77.97 and the night shift of 80. Average Results WWL (weighted workload) mental workload of employees in the morning, afternoon and evening shifts shows a high mental workload. The morning, afternoon and evening shifts have significant differences in physical needs, mental needs, time requirements, performance, stress levels and average WWL (weighted workload).","PeriodicalId":19392,"journal":{"name":"Nucleus","volume":"25 1","pages":""},"PeriodicalIF":3.7,"publicationDate":"2022-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83519111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tujuan penelitian ini adalah membuat sistem terkomputerisasi pada Bunda Laundry yang dapat membantu dalam proses administrasi dan pembuatan laporan keuangan, memudahkan pegawai untuk melihat ketersediaan stock bahan baku yang menunjang proses cuci setrika, membantu pegawai Bunda Laundry untuk meningkatkan kualitas pelayanan administrasi terhadap pelanggan sehingga dapat meningkatkan penjualan, dan mengetahui proses perancangan sistem informasi akuntansi pada Bunda Laundry . Data yang digunakan adalah data primer. Metode pengumpulan data berupa observasi langsung ke objek penelitian yaitu Bunda Laundry. Metode pengembangan sistem meliputi tahap perencanaan sistem, tahap analisis sistem, tahap perancangan sistem, tahap implementasi sistem, serta tahap pengujian dan penggunaan sistem. Hasil penelitian yaitu proses pelayanan konsumen dan pencatatan pada Bunda Laundry masih menggunakan sistem yang manual sehingga dibuatkan sistem yang terkomputerisasi. Prosedur pelayanan konsumen dimulai dari konsumen menaruh pakaiannya pada Bunda Laundry lalu bagian administrasi memberikan kuitansi pembayaran dan setelah itu pakaian konsumen diberikan kepada bagian produksi untuk di proses. Bila sudah selesai konsumen dapat mengambilnya dan membayarnya. Bagian administrasi akan merekap pembayaran tersebut dan membuat laporan keuangan yang akan diperiksa oleh pemilik laundry . Perancangan sistem ini dilakukan dengan mendeskripsikan masalah dan menganalisisnya menggunakan analisis PIECES. Setelah mengetahui kendala yang terjadi pada Bunda Laundry, penulis melakukan rancangan sistem menggunakan alur data flow diagram (DFD) dan membuat database dengan entity relationship diagram (ERD). Setelah semua hal dilalui, proses selanjutnya adalah menggambarkan rancangannya secara sederhana.
{"title":"Perancangan Sistem Informasi Akuntansi pada Bunda Laundry","authors":"Hapsari Widayani","doi":"10.37010/nuc.v3i1.716","DOIUrl":"https://doi.org/10.37010/nuc.v3i1.716","url":null,"abstract":"Tujuan penelitian ini adalah membuat sistem terkomputerisasi pada Bunda Laundry yang dapat membantu dalam proses administrasi dan pembuatan laporan keuangan, memudahkan pegawai untuk melihat ketersediaan stock bahan baku yang menunjang proses cuci setrika, membantu pegawai Bunda Laundry untuk meningkatkan kualitas pelayanan administrasi terhadap pelanggan sehingga dapat meningkatkan penjualan, dan mengetahui proses perancangan sistem informasi akuntansi pada Bunda Laundry . Data yang digunakan adalah data primer. Metode pengumpulan data berupa observasi langsung ke objek penelitian yaitu Bunda Laundry. Metode pengembangan sistem meliputi tahap perencanaan sistem, tahap analisis sistem, tahap perancangan sistem, tahap implementasi sistem, serta tahap pengujian dan penggunaan sistem. Hasil penelitian yaitu proses pelayanan konsumen dan pencatatan pada Bunda Laundry masih menggunakan sistem yang manual sehingga dibuatkan sistem yang terkomputerisasi. Prosedur pelayanan konsumen dimulai dari konsumen menaruh pakaiannya pada Bunda Laundry lalu bagian administrasi memberikan kuitansi pembayaran dan setelah itu pakaian konsumen diberikan kepada bagian produksi untuk di proses. Bila sudah selesai konsumen dapat mengambilnya dan membayarnya. Bagian administrasi akan merekap pembayaran tersebut dan membuat laporan keuangan yang akan diperiksa oleh pemilik laundry . Perancangan sistem ini dilakukan dengan mendeskripsikan masalah dan menganalisisnya menggunakan analisis PIECES. Setelah mengetahui kendala yang terjadi pada Bunda Laundry, penulis melakukan rancangan sistem menggunakan alur data flow diagram (DFD) dan membuat database dengan entity relationship diagram (ERD). Setelah semua hal dilalui, proses selanjutnya adalah menggambarkan rancangannya secara sederhana.","PeriodicalId":19392,"journal":{"name":"Nucleus","volume":"60 1","pages":""},"PeriodicalIF":3.7,"publicationDate":"2022-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77267081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anisa Rahmawati, Nayla Desviona, Tiara Puapita Sari
Unemployment is an employment problem that is often faced in every region, especially in Jambi Province. The indicator used to determine the number of unemployed is the open unemployment rate (TPT). High unemployment can be a major source of poverty that can lead to crime and hinder the process of economic development. As an initial precaution for the government before the increase in unemployment, which is to estimate the future TPT in Jambi Province. One of the methods used in estimating TPT in Jambi Province is the Double Exponential Smoothing Method from Holt. The measure of the forecast error uses the Mean Absolute Percent Error (MAPE). The aim is to obtain estimated TPT results in Jambi Province in the coming year, using Holt's Double Exponential Smoothing Method. The estimation results obtained, for TPT data there is a MAPE value of 18.96% with parameters = 0.9 and = 0.2. The estimation model obtained is = . The estimation results show that the TPT rate will continue to increase next year, it can be taken into consideration for the government in determining future plans to reduce TPT in order to accelerate economic development in Jambi Province.
{"title":"Estimation of the Open Unemployn Rate on Province Jambi Using the Double Exponential Smootthing","authors":"Anisa Rahmawati, Nayla Desviona, Tiara Puapita Sari","doi":"10.37010/nuc.v3i1.675","DOIUrl":"https://doi.org/10.37010/nuc.v3i1.675","url":null,"abstract":"Unemployment is an employment problem that is often faced in every region, especially in Jambi Province. The indicator used to determine the number of unemployed is the open unemployment rate (TPT). High unemployment can be a major source of poverty that can lead to crime and hinder the process of economic development. As an initial precaution for the government before the increase in unemployment, which is to estimate the future TPT in Jambi Province. One of the methods used in estimating TPT in Jambi Province is the Double Exponential Smoothing Method from Holt. The measure of the forecast error uses the Mean Absolute Percent Error (MAPE). The aim is to obtain estimated TPT results in Jambi Province in the coming year, using Holt's Double Exponential Smoothing Method. The estimation results obtained, for TPT data there is a MAPE value of 18.96% with parameters = 0.9 and = 0.2. The estimation model obtained is = . The estimation results show that the TPT rate will continue to increase next year, it can be taken into consideration for the government in determining future plans to reduce TPT in order to accelerate economic development in Jambi Province. ","PeriodicalId":19392,"journal":{"name":"Nucleus","volume":"1 1","pages":""},"PeriodicalIF":3.7,"publicationDate":"2022-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80006916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tujuan penelitian adalah untuk mengetahui seberapa besar pengaruh metode pembelajaran daring terhadap prestasi belajar Bahasa Inggris pada siswa kelas XI di SMKN 1 Bogor. Metode penelitian yang digunakan dalam penelitian ini adalah metode kuantitatif dengan pendekatan deskriptif untuk melihat seberapa besar pengaruh variabel bebas metode pembelajaran daring (X) terhadap variabel terikat prestasi belajar Bahasa Inggris (Y). Selanjutnya, teknik analisis yang digunakan yaitu teknik analisis koefisien korelasi, analisis koefisien determinasi, dan analisis regresi linear sederhana. Hasil penelitian ini menunjukkan bahwa nilai koefisien korelasi r sebesar -0,3178 yang berarti terdapat pengaruh antara metode pembelajaran daring terhadap prestasi belajar Bahasa Inggris pada siswa kelas XI di SMKN 1 Bogor. Koefisien determinasi diperoleh nilai sebesar 10% yang berarti variabel bebas metode pembelajaran daring (X) memberikan kontribusi sebesar 10% terhadap prestasi belajar Bahasa Inggris, sedangkan sisanya sebesar 90% dipengaruhi oleh variabel lain yang tidak diikutsertakan dalam penelitian ini. Persamaan linear sederhana dalam penelitian ini adalah Y = 96,889 - 0,137 X. Setelah itu, penelitian dilakukan dengan uji hipotesis menggunakan uji t secara parsial yang diperoleh nilai thitung = 2,743 dan ttabel pada df 67 (69-2) dengan taraf signifikan 0,05 secara dua arah sebesar 1,996 maka thitung > ttabel (2,743 > 1,996). Dengan adanya uji hipotesis ini, dapat disimpulkan bahwa terdapat pengaruh antara metode pembelajaran daring terhadap prestasi belajar Bahasa Inggris pada siswa kelas XI di SMKN 1 Bogor
{"title":"Pengaruh Metode Pembelajaran Daring terhadap Prestasi Belajar Bahasa Inggris pada Siswa Kelas XI di SMKN 1 Bogor","authors":"Wahyu Utama","doi":"10.37010/nuc.v3i1.899","DOIUrl":"https://doi.org/10.37010/nuc.v3i1.899","url":null,"abstract":"Tujuan penelitian adalah untuk mengetahui seberapa besar pengaruh metode pembelajaran daring terhadap prestasi belajar Bahasa Inggris pada siswa kelas XI di SMKN 1 Bogor. Metode penelitian yang digunakan dalam penelitian ini adalah metode kuantitatif dengan pendekatan deskriptif untuk melihat seberapa besar pengaruh variabel bebas metode pembelajaran daring (X) terhadap variabel terikat prestasi belajar Bahasa Inggris (Y). Selanjutnya, teknik analisis yang digunakan yaitu teknik analisis koefisien korelasi, analisis koefisien determinasi, dan analisis regresi linear sederhana. Hasil penelitian ini menunjukkan bahwa nilai koefisien korelasi r sebesar -0,3178 yang berarti terdapat pengaruh antara metode pembelajaran daring terhadap prestasi belajar Bahasa Inggris pada siswa kelas XI di SMKN 1 Bogor. Koefisien determinasi diperoleh nilai sebesar 10% yang berarti variabel bebas metode pembelajaran daring (X) memberikan kontribusi sebesar 10% terhadap prestasi belajar Bahasa Inggris, sedangkan sisanya sebesar 90% dipengaruhi oleh variabel lain yang tidak diikutsertakan dalam penelitian ini. Persamaan linear sederhana dalam penelitian ini adalah Y = 96,889 - 0,137 X. Setelah itu, penelitian dilakukan dengan uji hipotesis menggunakan uji t secara parsial yang diperoleh nilai thitung = 2,743 dan ttabel pada df 67 (69-2) dengan taraf signifikan 0,05 secara dua arah sebesar 1,996 maka thitung > ttabel (2,743 > 1,996). Dengan adanya uji hipotesis ini, dapat disimpulkan bahwa terdapat pengaruh antara metode pembelajaran daring terhadap prestasi belajar Bahasa Inggris pada siswa kelas XI di SMKN 1 Bogor","PeriodicalId":19392,"journal":{"name":"Nucleus","volume":"61 1","pages":""},"PeriodicalIF":3.7,"publicationDate":"2022-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82184411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
PT Home Center Indonesia atau Informa merupakan sebuah perusahaan perabot dimulai dari perabot rumah, kantor, hingga perabot ruang komersial. Tujuan penelitian ini untuk mengetahui jumlah respons pelanggan yang puas dan tidak puas dalam pelayanan jasa yang diberikan perusahaan perabot dan mengetahui faktor yang mempengaruhi kepuasan dan loyalitas untuk menyesuaikan pelayanan jasa yang diharapkan pelanggan. Dengan memberikan peningkatan loyalitas terhadap pelanggan dan menggunakan metode yang tepat, untuk mengukur seberapa peningkatan kepuasan dan loyalitas konsumen menggunakan metode data mining, Algoritma C4.5 dan CSI (Customer Satisfaction Index) yang menggunakan atribut kepuasan dan loyalitas pelanggan dalam penelitian yang mencakup dari harga, fasilitas, pelayanan dan loyalitas. atribut pendukung seperti kepentingan dan kepuasan dari (bukti fisik, kehandalan, empati, daya tanggap, kepastian) Adanya metode ini diharapkan dapat mengatasi masalah yang ada pada perusahaan agar dapat memprediksi dan mengevaluasi untuk terciptanya kepuasan dan loyalitas pelanggan yang diharapkan konsumen sesuai dengan target perusahaan. Hasil penelitian pengambilan responden pada pelanggan hasil dari nilai entropy dan gain sebesar entropy 0.971 dan gain sebesar 0,821 dalam perhitungan Algoritma C4.5. hasil perhitungan tingkat kepuasan pelanggan nilai CSI di PT Home Center Indonesia mendapatkan skor sebesar 42,14% yang berada pada rentang angka nilai indeks kepuasan pelanggan 35 – 50%. Dengan hasil kepuasan pelanggan secara keseluruhan berada pada kriteria “kurang puas”.
PT Home Center印度尼西亚或Informa是一家家具公司，从家具、办公室到商业空间家具不等。本研究的目的是确定家具公司对服务服务的满意和不满意客户的反应数量，并确定影响满意和忠诚以适应客户期望的服务的因素。通过增加对客户的忠诚并使用适当的方法，来衡量消费者使用数据挖掘方法、算法C4.5和CSI(算法C4.5)的满意度和忠诚度如何增加，这种方法在价格、设施、服务和忠诚方面利用了客户满意度和忠诚的属性。这种方法的支持属性，如利息、可靠性、同理心、感知能力和确定性，被认为可以解决公司内部存在的问题，以便预测和评估消费者期望的满意和忠诚的基础。客户调查结果显示，在计算算法C4.5的算法中，熵值和增益值为0.971，增益为0.821。印度尼西亚PT Home Center的CSI客户满意度计算结果在客户满意度指数35 - 50%范围内得分为42.14%。客户的总体满意度符合“不满意”的标准。
{"title":"Peningkatan Loyalitas Pelanggan pada PT Home Center Indonesia Menggunakan Metode Algoritma C4.5 dan Metode CSI (Customer Satisfaction Index)","authors":"Riri Cornellia, Anggi Oktaviani, Deny Wibisono","doi":"10.37010/nuc.v3i1.636","DOIUrl":"https://doi.org/10.37010/nuc.v3i1.636","url":null,"abstract":"PT Home Center Indonesia atau Informa merupakan sebuah perusahaan perabot dimulai dari perabot rumah, kantor, hingga perabot ruang komersial. Tujuan penelitian ini untuk mengetahui jumlah respons pelanggan yang puas dan tidak puas dalam pelayanan jasa yang diberikan perusahaan perabot dan mengetahui faktor yang mempengaruhi kepuasan dan loyalitas untuk menyesuaikan pelayanan jasa yang diharapkan pelanggan. Dengan memberikan peningkatan loyalitas terhadap pelanggan dan menggunakan metode yang tepat, untuk mengukur seberapa peningkatan kepuasan dan loyalitas konsumen menggunakan metode data mining, Algoritma C4.5 dan CSI (Customer Satisfaction Index) yang menggunakan atribut kepuasan dan loyalitas pelanggan dalam penelitian yang mencakup dari harga, fasilitas, pelayanan dan loyalitas. atribut pendukung seperti kepentingan dan kepuasan dari (bukti fisik, kehandalan, empati, daya tanggap, kepastian) Adanya metode ini diharapkan dapat mengatasi masalah yang ada pada perusahaan agar dapat memprediksi dan mengevaluasi untuk terciptanya kepuasan dan loyalitas pelanggan yang diharapkan konsumen sesuai dengan target perusahaan. Hasil penelitian pengambilan responden pada pelanggan hasil dari nilai entropy dan gain sebesar entropy 0.971 dan gain sebesar 0,821 dalam perhitungan Algoritma C4.5. hasil perhitungan tingkat kepuasan pelanggan nilai CSI di PT Home Center Indonesia mendapatkan skor sebesar 42,14% yang berada pada rentang angka nilai indeks kepuasan pelanggan 35 – 50%. Dengan hasil kepuasan pelanggan secara keseluruhan berada pada kriteria “kurang puas”.","PeriodicalId":19392,"journal":{"name":"Nucleus","volume":"21 1","pages":""},"PeriodicalIF":3.7,"publicationDate":"2022-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85419328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-29DOI: 10.1080/19491034.2022.2069071
Alexander F. Palazzo, Jomon Joseph, M. Lim, K. Thakur
ABSTRACT Dominant missense mutations in RanBP2/Nup358 cause Acute Necrotizing Encephalopathy (ANE), a pediatric disease where seemingly healthy individuals develop a cytokine storm that is restricted to the central nervous system in response to viral infection. Untreated, this condition leads to seizures, coma, long-term neurological damage and a high rate of mortality. The exact mechanism by which RanBP2 mutations contribute to the development of ANE remains elusive. In November 2021, a number of clinicians and basic scientists presented their work on this disease and on the interactions between RanBP2/Nup358, viral infections, the innate immune response and other cellular processes.
{"title":"Workshop on RanBP2/Nup358 and acute necrotizing encephalopathy","authors":"Alexander F. Palazzo, Jomon Joseph, M. Lim, K. Thakur","doi":"10.1080/19491034.2022.2069071","DOIUrl":"https://doi.org/10.1080/19491034.2022.2069071","url":null,"abstract":"ABSTRACT Dominant missense mutations in RanBP2/Nup358 cause Acute Necrotizing Encephalopathy (ANE), a pediatric disease where seemingly healthy individuals develop a cytokine storm that is restricted to the central nervous system in response to viral infection. Untreated, this condition leads to seizures, coma, long-term neurological damage and a high rate of mortality. The exact mechanism by which RanBP2 mutations contribute to the development of ANE remains elusive. In November 2021, a number of clinicians and basic scientists presented their work on this disease and on the interactions between RanBP2/Nup358, viral infections, the innate immune response and other cellular processes.","PeriodicalId":19392,"journal":{"name":"Nucleus","volume":"3 1","pages":"154 - 169"},"PeriodicalIF":3.7,"publicationDate":"2022-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79753279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-17DOI: 10.1080/19491034.2022.2047289
Ulrike Schweigel, Petros Batsios, A. Müller-Taubenberger, R. Gräf, Marianne Grafe
ABSTRACT Dictyostelium amoebae perform a semi-closed mitosis, in which the nuclear envelope is fenestrated at the insertion sites of the mitotic centrosomes and around the central spindle during karyokinesis. During late telophase the centrosome relocates to the cytoplasmic side of the nucleus, the central spindle disassembles and the nuclear fenestrae become closed. Our data indicate that Dictyostelium spastin (DdSpastin) is a microtubule-binding and severing type I membrane protein that plays a role in this process. Its mitotic localization is in agreement with a requirement for the removal of microtubules that would hinder closure of the fenestrae. Furthermore, DdSpastin interacts with the HeH/ LEM-family protein Src1 in BioID analyses as well as the inner nuclear membrane protein Sun1, and shows subcellular co-localizations with Src1, Sun1, the ESCRT component CHMP7 and the IST1-like protein filactin, suggesting that the principal pathway of mitotic nuclear envelope remodeling is conserved between animals and Dictyostelium amoebae.
{"title":"Dictyostelium spastin is involved in nuclear envelope dynamics during semi-closed mitosis","authors":"Ulrike Schweigel, Petros Batsios, A. Müller-Taubenberger, R. Gräf, Marianne Grafe","doi":"10.1080/19491034.2022.2047289","DOIUrl":"https://doi.org/10.1080/19491034.2022.2047289","url":null,"abstract":"ABSTRACT Dictyostelium amoebae perform a semi-closed mitosis, in which the nuclear envelope is fenestrated at the insertion sites of the mitotic centrosomes and around the central spindle during karyokinesis. During late telophase the centrosome relocates to the cytoplasmic side of the nucleus, the central spindle disassembles and the nuclear fenestrae become closed. Our data indicate that Dictyostelium spastin (DdSpastin) is a microtubule-binding and severing type I membrane protein that plays a role in this process. Its mitotic localization is in agreement with a requirement for the removal of microtubules that would hinder closure of the fenestrae. Furthermore, DdSpastin interacts with the HeH/ LEM-family protein Src1 in BioID analyses as well as the inner nuclear membrane protein Sun1, and shows subcellular co-localizations with Src1, Sun1, the ESCRT component CHMP7 and the IST1-like protein filactin, suggesting that the principal pathway of mitotic nuclear envelope remodeling is conserved between animals and Dictyostelium amoebae.","PeriodicalId":19392,"journal":{"name":"Nucleus","volume":"28 1","pages":"144 - 154"},"PeriodicalIF":3.7,"publicationDate":"2022-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73426864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-03DOI: 10.1080/19491034.2022.2043608
R. Pathak, Ashish Bihani, Rahul Sureka, Parul Varma, R. Mishra
ABSTRACT The study of nuclear matrix (NuMat) over the last 40 years has been limited to either isolated nuclei from tissues or cells grown in culture. Here, we provide a protocol for NuMat preparation in intact Drosophila melanogaster embryos and its use in dissecting the components of nuclear architecture. The protocol does not require isolation of nuclei and therefore maintains the three-dimensional milieu of an intact embryo, which is biologically more relevant compared to cells in culture. One of the advantages of this protocol is that only a small number of embryos are required. The protocol has been extended to larval tissues like salivary glands with little modification. Taken together, it becomes possible to carry out such studies in parallel to genetic experiments using mutant/transgenic flies. This protocol, therefore, opens the powerful field of fly genetics to cell biology in the study of nuclear architecture. Summary: Nuclear Matrix is a biochemically defined entity and a basic component of the nuclear architecture. Here we present a protocol to isolate and visualize Nuclear Matrix in situ in the Drosophila melanogaster and its potential applications.
{"title":"In situ nuclear matrix preparation in Drosophila melanogaster embryos/tissues and its use in studying the components of nuclear architecture","authors":"R. Pathak, Ashish Bihani, Rahul Sureka, Parul Varma, R. Mishra","doi":"10.1080/19491034.2022.2043608","DOIUrl":"https://doi.org/10.1080/19491034.2022.2043608","url":null,"abstract":"ABSTRACT The study of nuclear matrix (NuMat) over the last 40 years has been limited to either isolated nuclei from tissues or cells grown in culture. Here, we provide a protocol for NuMat preparation in intact Drosophila melanogaster embryos and its use in dissecting the components of nuclear architecture. The protocol does not require isolation of nuclei and therefore maintains the three-dimensional milieu of an intact embryo, which is biologically more relevant compared to cells in culture. One of the advantages of this protocol is that only a small number of embryos are required. The protocol has been extended to larval tissues like salivary glands with little modification. Taken together, it becomes possible to carry out such studies in parallel to genetic experiments using mutant/transgenic flies. This protocol, therefore, opens the powerful field of fly genetics to cell biology in the study of nuclear architecture. Summary: Nuclear Matrix is a biochemically defined entity and a basic component of the nuclear architecture. Here we present a protocol to isolate and visualize Nuclear Matrix in situ in the Drosophila melanogaster and its potential applications.","PeriodicalId":19392,"journal":{"name":"Nucleus","volume":"51 1","pages":"116 - 128"},"PeriodicalIF":3.7,"publicationDate":"2022-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86152385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-27DOI: 10.1080/19491034.2021.2024948
K. Raina, B. Rao
ABSTRACT Nuclear Speckles (NS) are phase-separated condensates of protein and RNA whose components dynamically coordinate RNA transcription, splicing, transport and DNA repair. NS, probed largely by imaging studies, remained historically well known as Interchromatin Granule Clusters, and biochemical properties, especially their association with Chromatin have been largely unexplored. In this study, we tested whether NS exhibit any stable association with chromatin and show that limited DNAse-1 nicking of chromatin leads to the collapse of NS into isotropic distribution or aggregates of constituent proteins without affecting other nuclear structures. Further biochemical probing revealed that NS proteins were tightly associated with chromatin, extractable only by high-salt treatment just like histone proteins. NS were also co-released with solubilised mono-dinucleosomal chromatin fraction following the MNase digestion of chromatin. We propose a model that NS-chromatin constitutes a “putative stable association” whose coupling might be subject to the combined regulation from both chromatin and NS changes. Abbreviations: NS: Nuclear speckles; DSB: double strand breaks; PTM: posttranslational modifications; DDR: DNA damage repair; RBP-RNA binding proteins; TAD: topologically associated domains; LCR: low complexity regions; IDR: intrinsically disordered regions.
{"title":"Mammalian nuclear speckles exhibit stable association with chromatin: a biochemical study","authors":"K. Raina, B. Rao","doi":"10.1080/19491034.2021.2024948","DOIUrl":"https://doi.org/10.1080/19491034.2021.2024948","url":null,"abstract":"ABSTRACT Nuclear Speckles (NS) are phase-separated condensates of protein and RNA whose components dynamically coordinate RNA transcription, splicing, transport and DNA repair. NS, probed largely by imaging studies, remained historically well known as Interchromatin Granule Clusters, and biochemical properties, especially their association with Chromatin have been largely unexplored. In this study, we tested whether NS exhibit any stable association with chromatin and show that limited DNAse-1 nicking of chromatin leads to the collapse of NS into isotropic distribution or aggregates of constituent proteins without affecting other nuclear structures. Further biochemical probing revealed that NS proteins were tightly associated with chromatin, extractable only by high-salt treatment just like histone proteins. NS were also co-released with solubilised mono-dinucleosomal chromatin fraction following the MNase digestion of chromatin. We propose a model that NS-chromatin constitutes a “putative stable association” whose coupling might be subject to the combined regulation from both chromatin and NS changes. Abbreviations: NS: Nuclear speckles; DSB: double strand breaks; PTM: posttranslational modifications; DDR: DNA damage repair; RBP-RNA binding proteins; TAD: topologically associated domains; LCR: low complexity regions; IDR: intrinsically disordered regions.","PeriodicalId":19392,"journal":{"name":"Nucleus","volume":"65 1","pages":"58 - 73"},"PeriodicalIF":3.7,"publicationDate":"2022-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91053833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-27DOI: 10.1080/19491034.2022.2038868
R. Jha, D. Levens, Fedor Kouzine
ABSTRACT The compaction of linear DNA into micrometer-sized nuclear boundaries involves the establishment of specific three-dimensional (3D) DNA structures complexed with histone proteins that form chromatin. The resulting structures modulate essential nuclear processes such as transcription, replication, and repair to facilitate or impede their multi-step progression and these contribute to dynamic modification of the 3D-genome organization. It is generally accepted that protein–protein and protein–DNA interactions form the basis of 3D-genome organization. However, the constant generation of mechanical forces, torques, and other stresses produced by various proteins translocating along DNA could be playing a larger role in genome organization than currently appreciated. Clearly, a thorough understanding of the mechanical determinants imposed by DNA transactions on the 3D organization of the genome is required. We provide here an overview of our current knowledge and highlight the importance of DNA and chromatin mechanics in gene expression.
{"title":"Mechanical determinants of chromatin topology and gene expression","authors":"R. Jha, D. Levens, Fedor Kouzine","doi":"10.1080/19491034.2022.2038868","DOIUrl":"https://doi.org/10.1080/19491034.2022.2038868","url":null,"abstract":"ABSTRACT The compaction of linear DNA into micrometer-sized nuclear boundaries involves the establishment of specific three-dimensional (3D) DNA structures complexed with histone proteins that form chromatin. The resulting structures modulate essential nuclear processes such as transcription, replication, and repair to facilitate or impede their multi-step progression and these contribute to dynamic modification of the 3D-genome organization. It is generally accepted that protein–protein and protein–DNA interactions form the basis of 3D-genome organization. However, the constant generation of mechanical forces, torques, and other stresses produced by various proteins translocating along DNA could be playing a larger role in genome organization than currently appreciated. Clearly, a thorough understanding of the mechanical determinants imposed by DNA transactions on the 3D organization of the genome is required. We provide here an overview of our current knowledge and highlight the importance of DNA and chromatin mechanics in gene expression.","PeriodicalId":19392,"journal":{"name":"Nucleus","volume":"21 1","pages":"94 - 115"},"PeriodicalIF":3.7,"publicationDate":"2022-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74343377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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