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Reinventing the oilseeds processing to extract oil while preserving the protein 重新发明油籽的加工方法,在榨油的同时保留蛋白质
OCL
Pub Date : 2021-01-01 DOI: 10.1051/OCL/2021001
P. Carré
The move towards more sustainable production and consumption patterns is a goal shared by a large part of society. In the oil-mill sector, whether it be animal feed or human food, this development should make it possible to improve the protein autonomy of the territories and to use proteins more efficiently. Current methods make some of the rapeseed proteins indigestible and leave most of the undesirable compounds from the seeds in resulting meals. Residual oil, fibrous envelopes, glucosinolates, phytic acid, phenolic compounds and oligosaccharides, as well as overcooking, however, have negative effects on the value of proteins. An important part of the article is devoted to reviewing these effects. Their extraction, combined with milder heat treatments, would provide excellent quality proteins to the market and generate value for the entire value chain. Efficient dehulling and mechanical extraction would be the prerequisites for this development. An ethanolic extraction revisited to exploit the secondary metabolites would then complete this process with, however, a limited effect on phytic acid. All these technologies have the advantage of not posing major acceptability problems. This development requires new investments in applied research to make this new approach to processing oilseeds possible and efficient.
迈向更可持续的生产和消费模式是社会大部分人的共同目标。在石油加工部门,无论是动物饲料还是人类食品,这一发展应该可以改善领土的蛋白质自主权,并更有效地利用蛋白质。目前的方法使一些油菜籽蛋白质难以消化,并将大多数不需要的化合物从种子中留下。然而,残油、纤维包膜、硫代葡萄糖苷、植酸、酚类化合物和低聚糖以及过度烹饪对蛋白质的价值有负面影响。这篇文章的一个重要部分致力于回顾这些影响。它们的提取,结合温和的热处理,将为市场提供优质的蛋白质,并为整个价值链创造价值。有效的脱壳和机械提取将是这一发展的先决条件。然而,对植酸的影响有限,再进行乙醇提取以利用次生代谢物来完成这一过程。所有这些技术的优点是不会造成主要的可接受性问题。这一发展需要在应用研究方面进行新的投资,以使这种加工油籽的新方法成为可能和有效。
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引用次数: 6
Relative toxicity of two natural compounds compared to abamectin against some soybean pests under period rates 两种天然化合物与阿维菌素对不同时期大豆害虫的相对毒性比较
OCL
Pub Date : 2021-01-01 DOI: 10.1051/OCL/2021018
Hala H. Al-Akhdar, Zeinab E. Ghareeb
A sustainable pest management in agro-ecosystems requires parallel assessments of pesticide and natural compounds to control target pests. In the present study, a semi-field experiment was conducted to evaluate the relative toxicity of abamectin (Abamax), humic acid, and Chitosan Nano-Particles (C.N.Ps) against four soybean pests: Tetranychus urticae, Eutetranychus orientalis, Bemisia tabaci, and Phenacoccus solenopsis. The experimental treatments were arranged in a split-plot design with three replicates, where treatments were assigned to main plots and pest populations under different periods in the subplots. The obtained results and graphs demonstrated that there were considerable differences between the total numbers of pests after different periods of treatment. Generally, E. orientalis recorded the minimum pest number at all. Different tested compounds demonstrated a considerable correlation between the densities of the pests. T. urticae exhibited a significant correlation with the other three pests (E. orientalis, B. tabaci, and P. solenopsis). E. orientalis did not correlate with B. tabaci and P. solenopsis. The humic acid recorded the best effect on T. urticae after 3 days with a reduction of 85.45% and E. orientalis after 7 days 65.55%. However, Chitosan Nano-Particles (C.N.Ps) was the best for E. orientalis after 14 days (mortality 74.36%). In contrast, abamectin (Abamax) had a general mean of reduction of 91.17% against T. urticae, whenever, these compounds are promising for controlling T. urticae, E. orientalis, B. tabaci, and P. solenopsis. These results may be a supporting method to overcome some soybean pests. The findings are discussed within the context of integrated management of soybean pests under semi-field conditions.
农业生态系统中可持续的有害生物管理需要对农药和天然化合物进行平行评估,以控制目标有害生物。本研究通过半田间试验研究了阿维菌素(Abamax)、腐植酸和壳聚糖纳米颗粒(C.N.Ps)对4种大豆害虫:棉花叶螨、东方叶螨、烟粉虱和索理芬锥虫的相对毒性。试验处理采用3个重复的分样设计,在主样地分配处理,在子样地分配不同时期的害虫种群。结果和图表表明,不同处理时期的害虫总数存在较大差异。总体而言,东方蓟的害虫数量最少。不同的测试化合物表明害虫的密度之间有相当大的相关性。荨麻疹与其他3种害虫(东方伊蚊、烟粉虱和扶桑拟虫)呈极显著相关。东方人与烟粉虱、扶桑粉虱无亲缘关系。腐植酸对荨麻疹3 d和7 d的效果最好,分别为85.45%和65.55%。而壳聚糖纳米颗粒(C.N.Ps)对14 d后的东方蓟的死亡率最高,为74.36%。而阿维菌素(Abamax)对荨麻疹的平均抑菌率为91.17%,在防治荨麻疹、东方螟、烟粉虱和扶桑拟虫等方面具有较好的应用前景。这些结果可作为防治某些大豆害虫的辅助方法。在半田条件下大豆害虫综合管理的背景下讨论了这些发现。
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引用次数: 1
Porous granules formation from oil crops by extrusion process: a theoretical perspective 油料作物挤压成型多孔颗粒的理论研究
OCL
Pub Date : 2021-01-01 DOI: 10.1051/ocl/2021032
Z. Meretukov, Evgeny P. Koshevoy, I. Shorstkii, C. Okpala
The introduction of extruders, in particular how it works and its principles, must be started from the definition of the extrusion process, the description of its development and types, as well as its functions and advantages. In order to understand the process of extrusion technology, it is necessary to go into detailed descriptions and discussions together with theoretical insights of the subject. In the present article, we discuss a theoretical perspective of production of porous granules from oil crops by extrusion technology. Let’s divide this theoretical perspective into three steps. At first, we study the modeling of statics of oil crop mix compressibility together with carbon dioxide. At second, we study the heat exchange and phase transformation during the stroke of oil crop extruder filled with CO2. At third, we observe the deformation of the oil crops during the extrusion process. There is a probability that extrusion process of oil crop mix with the hard phase of carbon dioxide provides technological way that does not overheat the cellular structure. Potentially, the equation of Van der Waals is able to describe the change in volume of oil crops, which may be related with range of factors associated with volume expansion, calculated by equations as the expansion of granule material takes place at the outlet of extruder matrix. The extrusion processing of a mix of oil crops and carbon dioxide in hard phase at initial stage may likely occur as temperature declines. The resulting values show good compliance of theoretical results to experimental data on the example of coriander seeds.
介绍挤出机,特别是它的工作原理和原理,必须从挤出工艺的定义,对其发展和类型的描述,以及它的功能和优点开始。为了了解挤压技术的过程,有必要进行详细的描述和讨论,并结合该主题的理论见解。本文讨论了利用挤压技术生产油料作物多孔颗粒的理论前景。让我们把这个理论视角分成三步。首先,我们研究了油料作物混合压缩性与二氧化碳的静力学建模。其次,研究了油料膨化机充入CO2冲程过程中的热交换和相变。第三,我们观察了油料作物在挤压过程中的变形。油料作物与二氧化碳硬相混合的挤压工艺有可能提供不使细胞结构过热的技术途径。范德华方程潜在地能够描述油料作物体积的变化,这可能与体积膨胀相关的一系列因素有关,当颗粒物料在挤出机基质出口膨胀时,由方程计算得到。当温度下降时,油料作物和二氧化碳在初始阶段处于硬相的混合物可能发生挤压加工。结果表明,以芫荽籽为例,理论结果与实验数据吻合较好。
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引用次数: 1
Assessment of genetic diversity in Moroccan sesame (Sesamum indicum) using ISSR molecular markers 利用ISSR分子标记评价摩洛哥芝麻的遗传多样性
OCL
Pub Date : 2021-01-01 DOI: 10.1051/OCL/2020072
Meriem El Harfi, Jamal Charafi, Karim Houmanat, H. Hanine, A. Nabloussi
There is a need for sesame (Sesamum indicum L.) breeding in Morocco to release performant and adapted varieties, which requires a large genetic variability in the germplasm to be used. In this context, genetic diversity of sesame populations from different locations in Tadla area was investigated using ISSR (Inter Sequence Simple Repeats) markers as a simple method to reveal polymorphism among them. A total of 130 individuals representing 31 populations were sampled. Twenty-four ISSR primers were used for analysis of individuals representing the 31 different sesame populations grown in different agroclimatic zones of Tadla, accounting for 90% of sesame cultivation area in Morocco. Indeed, seven primers showed legible and reproducible genomic profiles with an interesting number of bands. A total of 57 bands were obtained with ISSR primers, of which 47 were polymorphic. PIC (Polymorphic Information Content) ranged from 0.002 to 0.350, showing that ISSR markers are informative and relevant for discriminating the populations evaluated. The similarity coefficient of ISSR data ranged from 0.509 to 1, with an average of 0.870. The results obtained showed that Moroccan sesame populations are characterized by a low genetic diversity, suggesting a genetic proximity among them. Therefore, new germplasm should be either introduced from diverse geographical origins or created through mutagenesis breeding in order to broaden the existing genetic variability.
摩洛哥需要对芝麻(Sesamum indicum L.)进行育种,以释放高性能和适应性品种,这需要使用的种质资源具有很大的遗传变异性。在此背景下,利用ISSR (Inter Sequence Simple Repeats)标记分析了Tadla地区不同地区芝麻群体的遗传多样性。共有代表31个种群的130个个体被抽样。利用24条ISSR引物对占摩洛哥芝麻种植面积90%的Tadla不同农业气候带的31个不同芝麻种群进行个体分析。事实上,有7个引物显示出清晰且可重复的基因组图谱,其中条带数量非常有趣。利用ISSR引物共获得57条条带,其中47条为多态性条带。多态性信息含量(PIC)在0.002 ~ 0.350之间,表明ISSR标记具有信息性和相关性。ISSR数据的相似系数为0.509 ~ 1,平均值为0.870。结果表明,摩洛哥芝麻居群具有遗传多样性低的特点,表明它们之间存在遗传接近。因此,为了扩大现有的遗传变异,应该从不同的地理来源引进新的种质资源,或者通过诱变育种创造新的种质资源。
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引用次数: 11
Strategies for increasing lipid accumulation and recovery from Y. lipolytica: A review 增加脂质积累和脂质恢复的策略:综述
OCL
Pub Date : 2021-01-01 DOI: 10.1051/ocl/2021038
S. El Kantar, A. Khelfa, E. Vorobiev, M. Koubaa
Microbial-based biodiesel is produced by transesterification of lipids extracted from microbial cells, and is considered as a potential replacement of fossil fuel due to its advantages in reducing greenhouse gas emissions. Yarrowia lipolytica is one of the most studied oleaginous yeasts able to produce lipids under some fermentation conditions and is considered as a potential industrial host for biodiesel production. Several approaches have been evaluated to increase the economical attraction of biodiesel production from Y. lipolytica lipids. In this review, we highlighted the different strategies reported in the literature, allowing this yeast to achieve high lipid accumulation. These include metabolic engineering strategies, the use of low-cost effective substrates, and the optimization of the cultivation conditions for higher lipid productivity and less operating cost. We also summarized the most effective cell disruption technologies that improve the extraction efficiencies of lipids from Y. lipolytica.
微生物生物柴油是通过从微生物细胞中提取的脂质进行酯交换反应而生产的,由于其在减少温室气体排放方面的优势,被认为是化石燃料的潜在替代品。解脂耶氏菌是研究最多的产油酵母之一,在某些发酵条件下能够产生脂质,被认为是生物柴油生产的潜在工业宿主。已经对几种方法进行了评估,以增加从聚脂菌脂生产生物柴油的经济吸引力。在这篇综述中,我们强调了在文献中报道的不同策略,使这种酵母实现高脂质积累。这些包括代谢工程策略,使用低成本的有效底物,以及优化培养条件以提高脂质产量和降低操作成本。我们还总结了提高脂质体提取效率的最有效的细胞破坏技术。
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引用次数: 5
Old and new adventures with fatty acids and their oxylipins: The road towards personalised clinical nutrition 脂肪酸及其氧化脂素的新旧冒险:通往个性化临床营养的道路
OCL
Pub Date : 2021-01-01 DOI: 10.1051/ocl/2021034
Robert Gibson
After paying homage to the work of E. Chevreul, Prof. Robert Gibson went on in a lighthearted way to find similarities in the way they both approached their respective fields of research, as well as their way of life. Prof. Robert Gibson, who was awarded the 2021 Chevreul Medal, reported that “his huge delight was to witness the massive growth of lipid research and to have played a role in elucidating the role of dietary fats in the health of mothers and their babies”. Prof. Gibson highlighted some of the major results he collected from Australian clinical studies conducted on the role of omega-3 fatty acids on the health outcomes of mothers and their infants. He first discussed the role of fish oil on visual acuity of babies and demonstrated that infant formulas supplying more than 1% of linolenic acid (ALA) seemed adequate to ensure optimal visual and cognitive development of term infants. However, in preterm infants, whether there is a specific need for DHA above the benefit provided by ALA, still needs to be clarified. He reported a small beneficial impact on the cognitive development of preterm infants receiving DHA enriched breast milk of their mothers (1% of total fatty acids). He then discussed data from his large randomised clinical trials conducted on pregnant women receiving a DHA dietary treatment (800 mg/d DHA) or placebo, that suggested that DHA may decrease the risk of preterm birth (DOMInO trial, 2400 women). This effect was confirmed in the ORIP trial (5400 women) which found that preterm birth could be prevented by a DHA supplement treatment mainly in women with a singleton pregnancy who had a low omega-3 status in the first trimester. In the last part of his review, Robert Gibson described the use of a new low cost, rapid and efficient method to monitor changes in blood levels of omega-3 fatty acids with clinical outcomes: the Dried Blood Spots (DBS) technology. The validation of this technique has been demonstrated in large trials like N3RO and ORIP involving large cohorts of women, which could not have been obtained easily by classical analysis of lipids. He went on to point out that free fatty acids (generally not explored despite their importance in many metabolic disorders) and oxylipins, are both stable and easily identified when they are preserved in a dry state on a paper matrix (DBS), thus opening new fields of research. To conclude, the major impact of Prof. Robert Gibson’s work was identifying and overcoming one of the causes of early preterm birth (omega-3 deficiency), developing a tool to rapidly assess omega-3 status (the DBS technique) that together is close to being implemented into the world health system.
在向E. Chevreul的工作致敬之后,Robert Gibson教授以一种轻松的方式继续寻找他们在各自研究领域的相似之处,以及他们的生活方式。被授予2021年雪佛兰勋章的罗伯特·吉布森教授报告说,“他非常高兴看到脂质研究的大规模发展,并在阐明膳食脂肪在母亲和婴儿健康中的作用方面发挥了作用”。Gibson教授强调了他从澳大利亚临床研究中收集到的一些主要结果,这些研究是关于omega-3脂肪酸对母亲及其婴儿健康结果的作用。他首先讨论了鱼油对婴儿视力的作用,并证明提供超过1%的亚麻酸(ALA)的婴儿配方奶粉似乎足以确保足月婴儿的最佳视觉和认知发育。然而,在早产儿中,是否有比ALA提供的益处更特殊的DHA需求,仍然需要澄清。他报告说,接受母亲富含DHA的母乳(占总脂肪酸的1%)对早产儿的认知发育有轻微的有益影响。然后,他讨论了他的大型随机临床试验的数据,这些试验是对接受DHA饮食治疗(800毫克/天DHA)或安慰剂的孕妇进行的,表明DHA可以降低早产的风险(DOMInO试验,2400名妇女)。这种效果在ORIP试验(5400名妇女)中得到了证实,该试验发现,主要是在妊娠早期omega-3含量较低的单胎妊娠妇女中,DHA补充剂治疗可以预防早产。在回顾的最后一部分,Robert Gibson描述了一种新的低成本,快速和有效的方法来监测血液中omega-3脂肪酸水平的变化和临床结果:干血斑(DBS)技术。这项技术的有效性已经在N3RO和ORIP等大型试验中得到证实,这些试验涉及大量女性,这是通过传统的脂质分析无法轻易获得的。他接着指出,游离脂肪酸(尽管它们在许多代谢紊乱中很重要,但通常没有被探索过)和氧化脂素在干燥状态下保存在纸基质(DBS)上时都是稳定的,而且很容易识别,从而开辟了新的研究领域。总之,Robert Gibson教授工作的主要影响是确定并克服早产的原因之一(欧米茄-3缺乏症),开发一种快速评估欧米茄-3状态的工具(DBS技术),这些工具即将在世界卫生系统中实施。
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引用次数: 0
Fortification of chocolate using Moringa oleifera extract encapsulated in microemulsions 用辣木提取物包封在微乳剂中的巧克力强化
OCL
Pub Date : 2021-01-01 DOI: 10.1051/ocl/2021026
O. Kaltsa, A. Alibade, Georgia Batra, Eleni Bozinou, D. Makris, S. Lalas
The aim of the present study was to evaluate the physical and antioxidant properties of microemulsions containing Moringa oleifera leaf extract (MLE) produced by the means of a deep eutectic solvent. Selected microemulsions containing MLE were incorporated in chocolate products to enrich them. Their color properties including CIE L*, a*, b* parameters and whitening index (WI) along with DPPH radical scavenging activity were assessed during a period of 8 months. The antioxidant activity of microemulsions depended on the oil phase used, while it was unaffected by the concentration of MLE. Samples prepared with soybean oil as oil phase containing MLE presented the highest radical inhibition percentage (I% = 26.8–27.8%). Coconut microemulsions were finally incorporated at 2 and 4% w/w concentration into chocolate products, as coconut oil is a known cocoa butter substitute. Although the incorporation of MLE microemulsions did not affect the color properties of most of the chocolates, enriched products did not exhibit superior antioxidant activity compared to control samples.
研究了用深度共晶溶剂法制备的辣木叶提取物微乳的物理性能和抗氧化性能。选择含有MLE的微乳液加入到巧克力产品中,使其丰富。在8个月的时间里,评估了它们的颜色性能,包括CIE L*、a*、b*参数和美白指数(WI)以及DPPH自由基清除能力。微乳的抗氧化活性取决于油相,而不受MLE浓度的影响。以大豆油为油相的MLE对自由基的抑制率最高(I% = 26.8 ~ 27.8%)。椰子微乳液最终以2%和4%的w/w浓度加入到巧克力产品中,因为椰子油是一种已知的可可脂替代品。虽然MLE微乳剂的掺入对大多数巧克力的颜色特性没有影响,但与对照样品相比,强化产品没有表现出优越的抗氧化活性。
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引用次数: 6
High stearic sunflower oil: Latest advances and applications 高硬脂葵花籽油:最新进展和应用
OCL
Pub Date : 2021-01-01 DOI: 10.1051/ocl/2021022
J. Salas, Miguel A. Bootello, Enrique Martínez-Force, Mónica Venegas Calerón, R. Garcés
Regular sunflower oil is rich in linoleic acid. To improve its properties for different applications several genotypes with modified fatty acid compositions have been developed. Amongst them, the most remarkable have been high oleic and high stearic types. High stearic sunflower lines reported to date have been produced by traditional methods of breeding and mutagenesis. The mutations affected the expression of enzymes responsible for stearate desaturation in developing seeds. This trait has been combined with standard and high oleic backgrounds, giving high stearic lines with high contents of linoleic or oleic acids and thus different physical properties, increasing their functionality and potential applications. Nevertheless, for applications requiring plastic or confectionery fats, the oils have to be fractionated to obtain derived fats and butters with higher levels of solids. In the present review we present recent advances for the above mentioned topics related to high stearic sunflower oils.
普通的葵花籽油富含亚油酸。为了提高其性能以适应不同的应用,人们开发了几种带有修饰脂肪酸组成的基因型。其中,最显著的是高油酸和高硬脂酸类型。迄今为止报道的高硬脂向日葵系是通过传统的育种和诱变方法生产的。这些突变影响了发育中的种子中负责硬脂酸去饱和的酶的表达。这一特性与标准和高油酸背景相结合,使高硬脂酸线具有高亚油酸或油酸含量,从而具有不同的物理性质,增加了它们的功能和潜在的应用。然而,对于需要塑料或糖果脂肪的应用,必须对油进行分馏,以获得固体含量较高的衍生脂肪和黄油。本文综述了高硬脂葵花籽油在上述研究领域的最新进展。
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引用次数: 9
Extended surfactants and their tailored applications for vegetable oils extraction: An overview 扩展表面活性剂及其在植物油提取中的应用综述
OCL
Pub Date : 2021-01-01 DOI: 10.1051/OCL/2020062
Yancie Gagnon, H. Mhemdi, F. Delbecq, E. Hecke
The vegetable oil extraction process from seeds and nuts depends on mechanical and solvent (usually n-hexane) extractions. Despite the efficiency of n-hexane, its use is nowadays questioned due to health, environmental, and technological issues. As an alternative to hexane extraction, several greener solvents and extraction techniques have been developed and tested during the last decades. Among these alternatives, the Surfactant-Aqueous Extraction Process (SAEP) appears as a promising method. Initially developed for the petroleum sector, this method was then tested and optimized for vegetable oil extraction. Successful implementations at the laboratory scale led to slightly more than 90% oil yield, mainly by using so-called “extended surfactants”. Compare to conventional surfactants, these surfactants can efficiently solubilize a large amount of vegetable oil in water, despite the structural diversity and the bulkiness of vegetable oil molecules. The present review is devoted to extended surfactant applications to SAEP. This review summarizes and discusses the main findings related to the extended surfactant structures and properties, as well as the main experimental results on the SAEP, and the advantages and the current limitations towards a scaling-up of this promising process.
从种子和坚果中提取植物油的过程依赖于机械和溶剂(通常是正己烷)提取。尽管正己烷的效率很高,但由于健康、环境和技术问题,它的使用现在受到质疑。在过去的几十年里,一些更环保的溶剂和萃取技术已经被开发和测试,作为己烷萃取的替代品。在这些替代方法中,表面活性剂-水萃取法(SAEP)是一种很有前途的方法。这种方法最初是为石油部门开发的,后来经过测试并优化用于植物油提取。通过使用所谓的“扩展表面活性剂”,在实验室规模上的成功实施使原油产量略高于90%。与传统表面活性剂相比,尽管植物油分子结构多样,体积较大,但这些表面活性剂仍能有效地将大量植物油溶于水中。本文综述了表面活性剂在SAEP中的扩展应用。本文综述和讨论了扩展表面活性剂的结构和性能方面的主要研究成果,SAEP的主要实验结果,以及扩展表面活性剂的优势和目前的限制。
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引用次数: 9
Examining the role of institutional support in enhancing smallholder oilseed producers’ adaptability to climate change in Limpopo Province, South Africa 研究机构支持在提高南非林波波省小农油籽生产者对气候变化的适应能力方面的作用
OCL
Pub Date : 2021-01-01 DOI: 10.1051/OCL/2021004
Priscilla Ntuchu Kephe, B. Petja, K. Ayisi
Smallholder oilseed production constitutes a crucial component of rural economies and continues to face the consequences of a changing climate despite the increased levels of vulnerability. This paper assesses how smallholder oilseed farmers’ adaptive capacity in Limpopo is enhanced through various institutional support schemes within the context of increased climate extremes and their need to sustain production. Six hundred farmers were interviewed across three agro-ecological zones of Limpopo Province. Results show that some of the institutions’ current operations aimed at providing support to farmers do not adequately satisfy the farmers’ needs. A strong linear relationship was observed between the number of support types received by farmers and grain yield, suggesting that farmers who received more support types were relatively less vulnerable. Educational support is ranked as the most significant contributor to enhancing farmers’ adaptive capacity. This emphasizes the need for proper linkages between farmers’ choices of adaptive methods and the types of support needed. Therefore, this study provided a diagnosis of the gaps in essential types of institutional support needed to increase farmers’ resilience, which can be used as an input to beef up the policy and positioning to improve the adaptive capacity.
小农油籽生产是农村经济的重要组成部分,尽管脆弱性有所增加,但仍继续面临气候变化的后果。本文评估了在极端气候增加及其维持生产需求的背景下,林波波省油籽小农如何通过各种制度支持计划提高其适应能力。对林波波省三个农业生态区的600名农民进行了采访。结果表明,目前一些机构以支持农民为目的的业务不能充分满足农民的需求。农民接受的支持类型数量与粮食产量之间存在很强的线性关系,这表明农民接受的支持类型越多,相对而言就越不容易受到伤害。教育支持是提高农民适应能力的最重要因素。这强调需要在农民选择适应方法和所需支助类型之间建立适当的联系。因此,本研究对提高农民抗灾能力所需的基本制度支持类型的差距进行了诊断,可作为加强政策和定位以提高适应能力的投入。
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引用次数: 2
期刊
OCL
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