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Wine acidification methods: a review 葡萄酒酸化方法综述
IF 2.9 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2023-08-11 DOI: 10.20870/oeno-one.2023.57.3.7476
Claire Payan, A. Gancel, Michaël Jourdes, M. Christmann, Pierre-Louis Teissèdre
Global warming is directly linked to a lower concentration in organic acids in grape berries, leading to higher pHs in wine. Because of this lack of acidity, many important factors are impacted, as wine acidity and pH play a crucial role in various equilibriums. Indeed, the lower acidity and the higher pH modify the parameters of wine, such as free and molecular sulfur dioxide availability, colour and sensory aspects. Therefore, it is an ongoing challenge for winemakers to deal with wine acidification and thus preserve wine physico-chemical properties and prevent early spoilage due to microbiological instability induced by high pH. Different acidification methods are allowed by the OIV, chemical acidification being one the most common, followed by physical acidification and microbiological acidification. This review examines these three methods of acidification. The first part details chemical acidification and gives a complete description of various organic acids used in winemaking, and their different properties and regulations; the second part focuses on physical acidification, such as cation exchange resins and electrodialysis; and the last part briefly reviews the novelty of microbiological acidification in wine.
全球变暖与葡萄浆果中有机酸浓度降低直接相关,从而导致葡萄酒的pH值升高。由于酸度不足,许多重要因素都会受到影响,因为葡萄酒的酸度和pH在各种平衡中起着至关重要的作用。事实上,较低的酸度和较高的pH值改变了葡萄酒的参数,如游离和分子二氧化硫的可用性、颜色和感官方面。因此,酿酒师面临的一个持续挑战是处理葡萄酒酸化,从而保持葡萄酒的物理化学特性,防止由于高pH引起的微生物不稳定性而导致的早期腐败。OIV允许不同的酸化方法,化学酸化是最常见的酸化方法之一,其次是物理酸化和微生物酸化。这篇综述考察了这三种酸化方法。第一部分详细介绍了化学酸化,并对酿酒中使用的各种有机酸及其不同的性质和规律进行了完整的描述;第二部分着重介绍了物理酸化,如阳离子交换树脂和电渗析;最后简要评述了葡萄酒中微生物酸化的新颖性。
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引用次数: 1
Impact of harvest date on aroma compound composition of Merlot and Cabernet-Sauvignon must and wine in a context of climate change: a focus on cooked fruit molecular markers 气候变化背景下采收日期对梅洛、赤霞珠must和葡萄酒香气化合物组成的影响:熟果分子标记的研究
IF 2.9 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2023-07-31 DOI: 10.20870/oeno-one.2023.57.3.7458
Lucile Allamy, C. van Leeuwen, A. Pons
Choice of harvest date can modulate the aroma intensity of herbaceous/vegetal, fresh fruit and cooked fruit nuances, thus helping the winemaker to produce wine of different styles and balance. Relationships between the sensory attributes and aroma compounds of Merlot and Cabernet-Sauvignon grapes that were harvested sequentially and the resulting wines were evaluated for two vintages: 2012 and 2014. The fine-tuning of the harvest date modulated the aromas of the young wine and impacted the intensity of the cooked fruit aromas for both Merlot and Cabernet-Sauvignon. No correlation was observed between the must and wine in terms of the intensity of the cooked fruit aroma. In order to observe an impact on the intensity of the cooked fruit aroma it was necessary to delay the harvest date of Cabernet-Sauvignon by 4 to 12 days in 2012 and 2014 respectively. This value was 7 days for Merlot wines (2014 vintage). Furanones, lactone and ketones were well correlated with the perceived intensity of the cooked fruit aroma in the young wine. In addition, the highest concentrations of γ-nonalactone, 3-methyl-2,4-nonanedione, massoia lactone and furaneol were detected in Merlot wines made using late-harvested grapes. At the measured concentrations, these compounds can explain the aroma of cooked fruit detected in the red wines. Similar results were obtained for the Cabernet-Sauvignon wines made from grapes from a later harvest. The volatile compounds produced from the lipoxygenase pathway in the grapes were putatively involved in the evolution of the aroma of the red wines from sequential harvest dates, opening up the possibility of managing aroma profiles through harvest date decisions. These findings are important, as the identification of measurable key chemical parameters in grapes could provide grape growers and winemakers objective indicators for predicting final wine style and quality.
采收日期的选择可以调节草本/植物、新鲜水果和熟水果的香气强度,从而帮助酿酒师酿造出不同风格和平衡的葡萄酒。梅洛(Merlot)和赤霞珠(Cabernet-Sauvignon)葡萄的感官属性和香气化合物之间的关系,是顺序采收的,并对两个年份的葡萄酒进行了评估:2012年和2014年。采收日期的微调调节了年轻葡萄酒的香气,并影响了梅洛和赤霞珠熟果香气的强度。在煮熟的水果香气的强度方面,葡萄汁和葡萄酒之间没有观察到相关性。为了观察对熟果香气强度的影响,有必要将2012年和2014年赤霞珠的采收日期分别推迟4至12天。对于梅洛葡萄酒(2014年份),这个值是7天。呋喃酮、内酯和酮类与年轻葡萄酒中熟果香气的感知强度密切相关。此外,用晚采葡萄酿造的梅洛葡萄酒中γ-非内酯、3-甲基-2,4-壬二酮、马尾内酯和呋喃醇的含量最高。在测量的浓度下,这些化合物可以解释在红酒中检测到的煮熟水果的香气。用晚熟葡萄酿造的赤霞珠葡萄酒也得到了类似的结果。据推测,葡萄中脂氧合酶途径产生的挥发性化合物参与了连续采收日期后红酒香气的演变,这为通过采收日期决定管理香气谱提供了可能性。这些发现很重要,因为确定葡萄中可测量的关键化学参数可以为葡萄种植者和酿酒师提供预测最终葡萄酒风格和质量的客观指标。
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引用次数: 2
The transcriptionally active bacterial communities of grapevine rhizosphere in dependence on rootstock and scion variety 葡萄根际转录活性细菌群落对砧木和接穗品种的依赖
IF 2.9 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2023-07-25 DOI: 10.20870/oeno-one.2023.57.3.5547
L. Dries, S. Ratering, Simone Bussotti, O. Löhnertz, A. Vortkamp, S. Schnell
The rhizosphere is where crucial processes for the productivity of viticultural systems occur. The composition of the bacterial communities associated with the rhizosphere of grapevines is known to depend on plant genotype. However, the genotype of grafted grapevines differs between scion and rootstock; the role of each genotype is unclear. To untangle the effect of scion and rootstock, the rRNA (V4–V5 region of 16S rRNA) extracted from the rhizosphere of the grape varieties Riesling and Mueller-Thurgau ungrafted vs grafted on different rootstocks was sequenced. The bioinformatic analysis with tools designed to be robust for compositional data showed that the investigated rootstocks or scions or combinations, respectively, recruited bacterial communities with distinguishable traits. Statistical differences were revealed between ungrafted Riesling vs Mueller-Thurgau, between grafted Riesling vs ungrafted Riesling, and between ungrafted Mueller-Thurgau vs grafted Mueller-Thurgau. Thus, confirming the role of scion and rootstock genotype as a driver of the structure and composition of bacterial communities in the rhizosphere of grapevines.
根际是葡萄栽培系统生产力的关键过程发生的地方。已知与葡萄根际相关的细菌群落的组成取决于植物的基因型。然而,嫁接葡萄的基因型在接穗和砧木之间存在差异;每种基因型的作用尚不清楚。为了弄清接穗和砧木的影响,从葡萄品种雷司令和穆勒-图尔高的根际提取的rRNA (16S rRNA的V4-V5区)进行了测序。生物信息学分析表明,所研究的砧木、接穗或组合分别招募了具有可区分性状的细菌群落。未嫁接的雷司令与穆勒-图尔高之间、嫁接的雷司令与未嫁接的雷司令之间、未嫁接的穆勒-图尔高与嫁接的穆勒-图尔高之间存在统计学差异。由此,证实了接穗和砧木基因型对葡萄根际细菌群落结构和组成的驱动作用。
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引用次数: 0
Study of the effects of climatic conditions on the phenolic content and antioxidant activity of Austrian and Montenegrin red wines 气候条件对奥地利和黑山红葡萄酒酚类物质含量和抗氧化活性影响的研究
IF 2.9 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2023-07-21 DOI: 10.20870/oeno-one.2023.57.3.7450
R. Eder, Radmila Pajović Šćepanović, Danijela Raičević, T. Popović, K. Korntheuer, S. Wendelin, A. Forneck, C. Philipp
We evaluated the chemical composition and antioxidant activity of 30 red wines from Austria (cv. Cabernet-Sauvignon, Blaufraenkisch and Zweigelt) and 20 wines from Montenegro (cv. Cabernet-Sauvignon and Vranac) from three consecutive vintages: 2014, 2015 and 2016. We determined spectrophotometrically the contents of total phenol (TP), total anthocyanin (TA) and low- and high-molecular-weight proanthocyanidins (LMP and HMP respectively). We identified and quantified 18 phenolic compounds (hydroxycinnamic acids, hydroxybenzoic acids, stilbenes and flavan-3-ols) by high-performance liquid chromatography. In addition, we used Fourier-transform infrared spectroscopy for chemical analyses of the main wine parameters. Austrian autochthonous wines exhibited a similar chemical composition (alcohol 12.4 vol%, pH 3.38). Blaufraenkisch wines showed higher TP, HMP and LMP than Zweigelt wines; however, TA content was similar. Blaufraenkisch wines also showed higher phenolic acid, flavan-3-ol and stilbene contents than Zweigelt wines. Montenegrin autochthonous Vranac wines showed a typical chemical composition (alcohol 13.0 vol%, pH 3.42), and medium to high levels of phenols: TP, HMP, LMP, TA, HCA and flavan-3-ols. On the other hand, they showed a moderate stilbene content. Cabernet-Sauvignon wines from Austria and Montenegro exhibited some similarities in phenolic composition: TP, HMP, LMP and TA. There were notable variations in the phenolic acid and flavan-3-ol contents, especially the stilbene content, which was much higher in the Austrian wines than in the Montenegrin wines. These findings evidence an important impact of climatic conditions on these compounds. The antioxidant activity in all investigated wines was high and correlated strongly with TP, total phenol index, HMP and LMP in wines. The vintage influenced the chemical composition and content of all examined phenolic groups, except flavan-3-ols. Wines from the vintage with the best maturity (2015) contained the highest content of spectrophotometrically determined phenolic compounds and lowest level of phenolic acids and stilbenes. Principle component analysis showed that wines were mainly discriminated by variety and origin but not by vintage.
我们评估了奥地利30款红葡萄酒(赤霞珠、布劳夫兰基什和茨威格尔特)和黑山20款红葡萄酒的化学成分和抗氧化活性,这些葡萄酒分别来自2014年、2015年和2016年这三个连续年份。分光光度法测定了总酚(TP)、总花青素(TA)和低分子量和高分子量原花青素(分别为LMP和HMP)的含量。我们用高效液相色谱法鉴定并定量了18种酚类化合物(羟基肉桂酸、羟基苯甲酸、二苯乙烯和黄烷-3-醇)。此外,我们使用傅立叶变换红外光谱对葡萄酒的主要参数进行了化学分析。奥地利本土葡萄酒的化学成分相似(酒精含量12.4vol%,pH 3.38)。Blaufraenkisch葡萄酒的TP、HMP和LMP高于Zweigelt葡萄酒;但TA含量相近。Blaufraenkisch葡萄酒的酚酸、黄烷-3-醇和二苯乙烯含量也高于Zweigelt葡萄酒。黑山本土Vranc葡萄酒显示出典型的化学成分(酒精13.0体积%,pH 3.42),以及中高水平的酚类物质:TP、HMP、LMP、TA、HCA和黄烷-3-醇。另一方面,它们显示出中等的二苯乙烯含量。来自奥地利和黑山的赤霞珠葡萄酒在酚类成分上表现出一些相似之处:TP、HMP、LMP和TA。酚酸和黄烷-3-醇含量存在显著差异,尤其是二苯乙烯含量,奥地利葡萄酒的二苯乙烯含量远高于黑山葡萄酒。这些发现证明了气候条件对这些化合物的重要影响。所有葡萄酒的抗氧化活性都很高,并且与葡萄酒中的TP、总酚指数、HMP和LMP密切相关。年份影响了除黄烷-3-醇外的所有酚类化合物的化学组成和含量。成熟度最佳年份(2015年)的葡萄酒中,分光光度法测定的酚类化合物含量最高,酚酸和二苯乙烯含量最低。主成分分析表明,葡萄酒主要受品种和产地的影响,而不受年份的影响。
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引用次数: 0
Xylem water transport is influenced by age and winter pruning characteristics in grapevine (Vitis vinifera) 葡萄(Vitis vinifera)树龄和冬季修剪特征对木质部水分运输的影响
IF 2.9 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2023-07-20 DOI: 10.20870/oeno-one.2023.57.3.7452
Marion Claverie, P. Lecomte, Gaël Delorme, V. Dumot, O. Jacquet, H. Cochard
In order to investigate the effect of age and pruning characteristics on grapevine hydraulic conduction, a study was carried out between 2017 and 2020. Two pruning regimes (respecting or not sap flow pathways) and two vine age levels (older and younger vine plants) were considered and compared on two different vineyard plots located in French north-east Jura region and in south-west Bordeaux one. The assessment of pruning characteristics in relation to sap flow pathway was based on a visual characterization of the external wood aspect of the trunk and arms and consisted of a set of criteria involving the number, size and position of pruning wounds.Sap flow measurements of entire vine plants were carried out using the Xyl’em® tool, as well as an assessment of the necrotized, living and conductive xylem area in the trunks and arms after Phloxine staining. The biomass of the vegetation was also assessed.Results showed that vines pruned without considering the sap flow pathways had a 40-to-50% less conductive sap flow than vines pruned taking into account the sap pathway. No difference was observed with vine age within each pruning regime. However, for the vineyard plot where the amount of conductive xylem area was assessed, older vines that were not pruned to respect the sap pathways showed a smaller area of living wood as well as conductive wood than the older ones pruned to respect the sap flows. The amount of living and conductive areas of these vines was equivalent to that of younger vines pruned to respect sap pathways. These older vines also showed less vegetative biomass.These results show that pruning without taking into account the sap pathways has a negative impact on the conduction of xylem sap pathways in grapevines, both in terms of hydraulic efficiency and quantity of living and conducting tissues. However, the possible consequences of these reductions on grapevine physiological functions still need to be further investigated.
为了研究树龄和修剪特征对葡萄水分传导的影响,在2017年至2020年间进行了一项研究。在法国东北部汝拉地区和波尔多西南部的两个不同葡萄园地块上,考虑并比较了两种修剪制度(尊重或不尊重树液流动路径)和两种葡萄树龄水平(较老和较年轻的葡萄植株)。与树液流动路径相关的修剪特征评估是基于树干和手臂外部木材外观的视觉特征,并包括一组涉及修剪伤口数量、大小和位置的标准。使用Xyl’em®工具对整个藤蔓植物的汁液流量进行了测量,并在Phloxine染色后对树干和枝条中坏死、存活和导电的木质部区域进行了评估。还对植被的生物量进行了评估。结果表明,在不考虑树液流动路径的情况下修剪的葡萄藤的传导性树液流动比考虑树液路径的葡萄藤少40-50%。在每个修剪制度下,没有观察到葡萄藤年龄的差异。然而,对于评估传导木质部面积的葡萄园地块,未按照树液路径修剪的较老葡萄藤显示出比按照树液流动修剪的较旧葡萄藤更小的活木和传导木面积。这些葡萄藤的生活区和传导区的数量相当于为尊重树液路径而修剪的年轻葡萄藤的面积。这些较老的葡萄藤也显示出较少的营养生物量。这些结果表明,在不考虑树液通道的情况下进行修剪,无论是从水力效率还是活组织和传导组织的数量来看,都会对葡萄木质部树液通道传导产生负面影响。然而,这些减少对葡萄生理功能的可能影响仍有待进一步研究。
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引用次数: 0
Impact of phytosterol addition on fermentation progress and volatile compounds synthesis during alcoholic fermentation in synthetic and natural grape musts 添加植物甾醇对合成和天然葡萄汁酒精发酵过程中发酵过程和挥发性化合物合成的影响
IF 2.9 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2023-07-19 DOI: 10.20870/oeno-one.2023.57.3.7479
E. Casalta, Carla Sabatier, Giovana Girardi-Piva, Gabriel Dournes, A. Roland, J. Mouret
Lipid nutrition is an important factor for yeast during alcoholic fermentation. Although recent research reports have revisited the role of sterols during alcoholic fermentation, our knowledge of lipids assimilation and volatile compound biogenesis remains partial. This study aimed to find out more about the impact of grape must phytosterol content on fermentative kinetics, nitrogen assimilation by yeast and fermentative aroma synthesis. To that end, experimental fermentations were performed in synthetic and Chardonnay musts supplemented with different phytosterol concentrations (0, 1, 3 and 5 mg/L). Sterols addition significantly increased the maximum CO2 production rate while reducing fermentation duration. This can be explained by higher nitrogen assimilation by yeast due to sterols, which leads to higher yeast growth and better viability at the end of the fermentation process. Regarding the aromatic profile, sterol addition also significantly increased acetate esters, ethyl esters, fusel alcohols and medium-chain fatty acids production. These new advances highlight the major role of phytosterols in fermentation control and wine aroma profile.
油脂营养是酵母在酒精发酵过程中的一个重要因素。尽管最近的研究报告重新探讨了甾醇在酒精发酵过程中的作用,但我们对脂质同化和挥发性化合物生物发生的了解仍然是部分的。本研究旨在了解葡萄须植物甾醇含量对发酵动力学、酵母氮同化和发酵香气合成的影响。为此,在添加了不同植物甾醇浓度(0、1、3和5 mg/L)的合成和霞多丽霉中进行了实验发酵。甾醇的添加显著提高了最大CO2生产速率,同时缩短了发酵时间。这可以通过固醇引起的酵母对氮的更高同化来解释,固醇导致酵母生长更高,在发酵过程结束时具有更好的生存能力。关于芳香族特征,添加甾醇也显著增加了乙酸酯、乙酯、杂醇和中链脂肪酸的产量。这些新进展突出了植物甾醇在发酵控制和葡萄酒香气特征方面的主要作用。
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引用次数: 0
How to better estimate bunch number at vineyard level? 如何更好地估计葡萄园级别的葡萄串数量?
IF 2.9 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2023-07-17 DOI: 10.20870/oeno-one.2023.57.3.7404
B. Oger, C. Laurent, P. Vismara, B. Tisseyre
Despite the extensive use of sampling to estimate the average number of grape bunches per vine, there is no clearly established sampling protocol that can be used as a reference when performing these estimations. Each practitioner therefore has their own sampling protocol. This study characterised the effect of differences between sampling protocols in terms of estimation errors. The goal was to identify the most efficient practices that will improve the early estimation of an important yield component: average bunch number. First, the appropriateness of including non-productive vines (i.e., dead and missing vines) in the sampling protocol was tested; the objective was to determine whether it is relevant to estimate two yield components simultaneously. Second, sampling protocols with sampling sites of varying size were compared to determine how the spatial distribution of observations and potential spatial autocorrelation affect estimation error. Third, a new confidence interval for estimation error was determined to express expected error as a percentage. It aimed at designing a new tool for finding the best sample size in an operational context. Tests were performed on two vineyards in the South of France, in which the number of bunches per vine had been exhaustively determined on all the plants before flowering. The results show that the simultaneous estimation of number of bunches and proportion of dead and missing vines increased the estimation errors by a factor of 2. Despite the low spatial autocorrelation of bunch number, the results show that the observation must be spread across at least 2 or 3 sampling sites to reduce estimation errors. Finally, the confidence intervals expressed as a percentage were validated and used to define an adequate sample size based on a compromise between the expected precision and the variability observed in the first measurements.
尽管广泛使用采样来估计每株葡萄的平均葡萄串数量,但在进行这些估计时,没有明确制定的采样协议可作为参考。因此,每个从业者都有自己的采样方案。这项研究从估计误差的角度描述了采样协议之间差异的影响。目标是确定最有效的实践,以改进对一个重要产量组成部分的早期估计:平均束数。首先,测试了将非生产性葡萄藤(即死亡和缺失的葡萄藤)纳入采样方案的适当性;目的是确定同时估计两个产量成分是否相关。其次,比较了具有不同大小采样点的采样协议,以确定观测的空间分布和潜在的空间自相关如何影响估计误差。第三,确定了一个新的估计误差置信区间,将预期误差表示为百分比。它旨在设计一种新的工具,以便在操作环境中找到最佳样本量。在法国南部的两个葡萄园进行了测试,在开花前,对所有植物的每株葡萄的束数进行了详尽的测定。结果表明,同时估计葡萄串的数量和死亡葡萄藤和缺失葡萄藤的比例会使估计误差增加2倍。尽管束数的空间自相关较低,但结果表明,观测必须分布在至少2或3个采样点,以减少估计误差。最后,验证了以百分比表示的置信区间,并根据第一次测量中观察到的预期精度和变异性之间的折衷,使用置信区间来定义适当的样本量。
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引用次数: 1
Migration of polyphenols from natural and microagglomerated cork stoppers to hydroalcoholic solutions and their sensory impact 多酚从天然和微凝集软木塞向水醇溶液的迁移及其感官影响
IF 2.9 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2023-07-10 DOI: 10.20870/oeno-one.2023.57.3.7393
A. Gancel, Michaël Jourdes, A. Pons, Pierre-Louis Teissèdre
During bottling aging, the wine comes into contact with the cork stopper due to the horizontal position of the bottle. The release of compounds, such as cork phenolic compounds, thus take place between the cork and the wine, depending on the type of cork stopper and the surface treatments applied. Many publications describe the extraction of these phenolic compounds in wine or hydroalcoholic solutions from natural corks, but few address microagglomerated corks, which are increasingly used by winemakers to seal their bottles. The aim of this study was therefore to compare the polyphenols, mainly hydrolysable tannins, transferred from natural and microagglomerated corks treated with supercritical CO2 into hydroalcoholic solutions. For this purpose, polyphenols released in macerates of natural and microagglomerated cork stoppers were identified and quantified by HPLC-DAD-ESI-QQQ. Suberic acid was also quantified. In this study, despite the high intra-“natural cork stopper” variability, significant differences were found between both types of stoppers for all polyphenols, the agglomerated corks releasing significantly less polyphenols; i.e., 25 times less. In contrast, suberic acid was extracted from both types of corks in similar concentrations; therefore, its extractability was not impacted by the type of stopper. A sensory profile was also carried out on the macerates. Macerates of natural cork stoppers were perceived with notes of “cardboard, dust, plank, wood” and “cork taint” significantly higher than supercritical CO2 treated microagglomerated cork stopper macerates. Moreover, the natural cork macerate with the highest content in polyphenol was perceived as being more bitter than that of microagglomerated cork stoppers.
在装瓶陈化过程中,由于瓶子的水平位置,葡萄酒会与软木塞接触。因此,化合物的释放,如软木酚类化合物,发生在软木塞和葡萄酒之间,这取决于软木塞的类型和所应用的表面处理。许多出版物描述了从天然软木塞中提取葡萄酒或水醇溶液中的这些酚类化合物,但很少涉及微凝集软木塞,酿酒师越来越多地使用微凝集软木来密封瓶子。因此,本研究的目的是比较用超临界CO2处理的天然和微凝聚软木塞转移到水醇溶液中的多酚,主要是可水解的单宁。为此,通过HPLC-DAD-ESI-QQQ对天然软木塞和微凝集软木塞的浸渍液中释放的多酚进行了鉴定和定量。亚基酸也进行了定量。在这项研究中,尽管“天然软木塞”内部的变异性很高,但两种类型的软木塞在所有多酚方面都存在显著差异,结块的软木塞释放的多酚明显较少;即减少25倍。相反,木栓酸是以相似的浓度从两种类型的软木塞中提取的;因此,其可提取性不受塞子类型的影响。还对显微组分进行了感官分析。天然软木塞的Macerates带有“纸板、灰尘、木板、木材”和“软木污渍”的痕迹,显著高于超临界CO2处理的微凝聚软木塞浸渍物。此外,多酚含量最高的天然软木浸渍剂比微凝集软木塞更苦。
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引用次数: 0
Investigation of powdery & downy mildew segregation in Bogazkere hybrids of Turkish wine grape 土耳其酿酒葡萄Bogazkere杂交品种白粉病与霜霉病分离的研究
IF 2.9 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2023-07-04 DOI: 10.20870/oeno-one.2023.57.3.7064
Metehan Gunhan, A. Jermakow, Nayana Arunasiri, I. Dry
Powdery mildew (Erysiphe necator syn. Uncinula necator) and downy mildew (Plasmapora viticola) are the most harmful diseases to grapevine production in Turkey. The aim of this study was to generate hybrids between the susceptible Turkish wine grape variety ‘Bogazkere’ and powdery and downy mildew resistant microvine breeding lines and evaluate their resistance to these important diseases. Marker Assisted Selection (MAS) was used to determine the inheritance of powdery and downy mildew resistance and flower sex loci of the 36 F1 progeny plants obtained by cross-pollination at genotypic level. Based on the PCR results, phenotyping and leaf bioassay were performed to validate initial MAS for 11 and 9 representative progeny with four resistant and susceptible controls. As a result, two tall powdery and downy mildew-resistant vines, three powdery mildew-resistant microvines and two downy mildew-resistant microvines were obtained. The MAS results were also validated by leaf bioassays, which confirmed that resistance genes segregated to the F1 progeny are functional and provide resistance. To the best of our knowledge, this research provides the first photographic validation of the powdery and downy mildew resistance of the Turkish variety ‘Bogazkere’. Our findings could potentially provide the wine industry with a resistant wine grape cultivar and an addition to the wine grape germplasm, which could contribute to future breeding programs. Most importantly, the success of the resistance genes segregated into the candidate progeny could help significantly reduce the use of chemicals against grapevine pathogens.
白粉菌(Erysiphe necator syn.Uncinula necator)和霜霉菌(Plasmapora viticola)是土耳其葡萄生产中危害最大的疾病。本研究的目的是在易感的土耳其酿酒葡萄品种“Bogazkere”和抗白粉霜霉微绒毛育种系之间产生杂交种,并评估它们对这些重要疾病的抗性。利用标记辅助选择(MAS)技术,在基因型水平上测定了36个F1代异花授粉植株的抗白粉霜霉性和花性基因座的遗传。基于PCR结果,进行表型分析和叶片生物测定,以验证具有4个抗性和易感对照的11个和9个代表性后代的初始MAS。结果获得了两株高大的抗白粉霜葡萄藤、三株抗白粉菌微绒毛和两株抗霜霉微绒毛。MAS结果也通过叶片生物测定进行了验证,证实分离到F1后代的抗性基因具有功能并提供抗性。据我们所知,这项研究首次用照片验证了土耳其品种“Bogazkere”的抗粉状和霜霉菌性。我们的发现可能为葡萄酒行业提供一种具有抗性的酿酒葡萄品种,并为酿酒葡萄种质提供补充,这可能有助于未来的育种计划。最重要的是,将抗性基因分离到候选后代中的成功可能有助于显著减少对抗葡萄病原体的化学物质的使用。
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引用次数: 0
An operational model for capturing grape ripening dynamics to support harvest decisions 捕获葡萄成熟动态以支持收获决策的操作模型
IF 2.9 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2023-06-26 DOI: 10.20870/oeno-one.2023.57.2.7399
C. van Leeuwen, A. Destrac-Irvine, M. Gowdy, Laura Farris, P. Pieri, Laura Marolleau, G. Gambetta
Grape ripening is a critical phenological phase during which many metabolites that impact wine quality accumulate in the berries. Major changes in berry composition include a rapid increase in sugar and a decrease in malic acid content and concentration. Its duration is highly variable depending on grapevine variety, climatic parameters, soil type and management practices. Together with the timing of mid-veraison, this duration determines when grapes can be harvested.Viticulturists and winemakers monitor the sugar-to-total acidity ratio (S/TA) during grape ripening and start harvesting grapes when this ratio reaches the optimum value for the desired wine style. The S/TA ratio evolves linearly as a function of thermal summation during the first four weeks following the onset of ripening. The linearity of the evolution of the S/TA ratio as a function of thermal time during the first four weeks following mid-veraison is applied in this study on two large data sets encompassing (1) 53 varieties studied during 10 years with two to four replicates for each combination of year and cultivar and (2) two varieties, cultivated on three soil types over 13 years. Grape ripening speed is highly variable. The effects of the year impact ripening speed more than the effects of the soil or the variety, although all three effects are highly significant. Grape ripening speed decreases with berry weight and also varies with vine water status. By using this approach, viticulturists and winemakers can assess four weeks after mid-veraison, for each individual vineyard parcel, at what speed grape ripening progresses. Combined with precise mid-veraison scoring, expertise from previous vintages and complementary approaches like sensory assessment of berries, it allows harvest date estimates to be fine-tuned. The results of this study can also be used to identify slow ripening varies, which are better performing in warm climates and, thus, better adapted to climate change.
葡萄成熟是一个关键的酚期,在此期间,许多影响葡萄酒质量的代谢产物在浆果中积累。浆果成分的主要变化包括糖的快速增加以及苹果酸含量和浓度的降低。其持续时间因葡萄品种、气候参数、土壤类型和管理实践而异。再加上葡萄成熟中期的时间,这个持续时间决定了葡萄何时可以收获。葡萄种植者和酿酒师在葡萄成熟过程中监测糖与总酸度的比值(S/TA),并在该比值达到所需葡萄酒风格的最佳值时开始收获葡萄。在成熟开始后的前四周内,S/TA比率作为热总和的函数线性演变。在这项研究中,S/TA比率作为中期后前四周热时间函数的线性演变应用于两个大数据集,包括(1)在10年内研究的53个品种,年份和品种的每个组合有两到四个重复;(2)在三种土壤类型上培养13年的两个品种。葡萄成熟速度变化很大。年份的影响比土壤或品种的影响更能影响成熟速度,尽管这三种影响都非常显著。葡萄成熟速度随浆果重量的增加而降低,也随葡萄水分状况的变化而变化。通过使用这种方法,葡萄种植者和酿酒师可以评估葡萄成熟中期后四周,每个葡萄园地块的葡萄成熟速度。结合精确的中白葡萄酒评分、以前年份的专业知识以及浆果感官评估等互补方法,它可以对收获日期的估计进行微调。这项研究的结果也可以用来识别慢熟品种,这些品种在温暖的气候中表现更好,从而更好地适应气候变化。
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引用次数: 1
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OENO One
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