Pub Date : 2022-03-07DOI: 10.1080/01919512.2022.2040353
F. Oliveira, Laerte Marlon Conceição dos Santos, E. S. da Silva, Letícia de Alencar Pereira Rodrigues, Paulo Roberto Freitas Neves, Greta Almeida Fernandes Moreira, Gabriela Matheus Monteiro Lobato, Carlos Nascimento, Marcelo Gerhardt, Alex Álisson Bandeira Santos, Luís Alberto Brêda Mascarenhas, Bruna Aparecida Souza Machado
ABSTRACT Due to the high recurrence of microbial infections, developing new technologies for preventing the dissemination of pathogens is essential, especially to prevent infection in humans. Thus, devices for the decontamination of surfaces reduce not only the spread of pathogens in the environment, but provide greater security and protection for communities. Ozone (O3) is a substance capable of reducing or eliminating several types of microorganisms owing to its biocidal capacity, including when it is dissolved in water. The objective of this study was to develop an instant decontamination device using ozonated water. To confirm its biocidal action and verify the device’s efficacy, the reduction of the microbial load of important pathogens on personal protective equipment (PPE) was assessed. In addition, in order to confirm the biocidal action of ozonated water against SARS-CoV-2, in vitro tests on a viral model of Gammacoronavirus were performed. The results showed the efficacy of ozonated water in the disinfection device at concentration ranges of 0.3–0.6 mg/L and 0.7–0.9 mg/L of ozonated water, with growth reductions above 2 log10 for both concentration ranges tested and inactivation fractions above 60% (0.3–0.6 mg/L) and 80% (0.7–0.9 mg/L), with a high proportion of the tested PPE showing 100% microbial reduction. In vitro results for the evaluation of ozonated water in a viral model showed a 99.9% reduction percentage in the concentration range of 0.3 to 0.5 mg/L and a 99% reduction in the concentration range of 0.6 to 0.8 mg/L, with a 5.10 log EDI50/mL and 6.95 log EDI50/mL reduction, respectively. The instant decontamination system developed in this study proved effective for microbial reduction, and we confirmed the potential of ozonated water as a biocidal agent. Therefore, the proposed decontamination device could be considered as a tool for reducing contamination on surfaces using ozonated water.
{"title":"Disinfecting Efficacy of an Ozonated Water Spray Chamber: Scientific Evidence of the Total and Partial Biocidal Effect on Personal Protective Equipment and in Vitro Analysis of a Viral Experimental Model","authors":"F. Oliveira, Laerte Marlon Conceição dos Santos, E. S. da Silva, Letícia de Alencar Pereira Rodrigues, Paulo Roberto Freitas Neves, Greta Almeida Fernandes Moreira, Gabriela Matheus Monteiro Lobato, Carlos Nascimento, Marcelo Gerhardt, Alex Álisson Bandeira Santos, Luís Alberto Brêda Mascarenhas, Bruna Aparecida Souza Machado","doi":"10.1080/01919512.2022.2040353","DOIUrl":"https://doi.org/10.1080/01919512.2022.2040353","url":null,"abstract":"ABSTRACT Due to the high recurrence of microbial infections, developing new technologies for preventing the dissemination of pathogens is essential, especially to prevent infection in humans. Thus, devices for the decontamination of surfaces reduce not only the spread of pathogens in the environment, but provide greater security and protection for communities. Ozone (O3) is a substance capable of reducing or eliminating several types of microorganisms owing to its biocidal capacity, including when it is dissolved in water. The objective of this study was to develop an instant decontamination device using ozonated water. To confirm its biocidal action and verify the device’s efficacy, the reduction of the microbial load of important pathogens on personal protective equipment (PPE) was assessed. In addition, in order to confirm the biocidal action of ozonated water against SARS-CoV-2, in vitro tests on a viral model of Gammacoronavirus were performed. The results showed the efficacy of ozonated water in the disinfection device at concentration ranges of 0.3–0.6 mg/L and 0.7–0.9 mg/L of ozonated water, with growth reductions above 2 log10 for both concentration ranges tested and inactivation fractions above 60% (0.3–0.6 mg/L) and 80% (0.7–0.9 mg/L), with a high proportion of the tested PPE showing 100% microbial reduction. In vitro results for the evaluation of ozonated water in a viral model showed a 99.9% reduction percentage in the concentration range of 0.3 to 0.5 mg/L and a 99% reduction in the concentration range of 0.6 to 0.8 mg/L, with a 5.10 log EDI50/mL and 6.95 log EDI50/mL reduction, respectively. The instant decontamination system developed in this study proved effective for microbial reduction, and we confirmed the potential of ozonated water as a biocidal agent. Therefore, the proposed decontamination device could be considered as a tool for reducing contamination on surfaces using ozonated water.","PeriodicalId":19580,"journal":{"name":"Ozone: Science & Engineering","volume":"69 1","pages":"111 - 129"},"PeriodicalIF":2.7,"publicationDate":"2022-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90417988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-03DOI: 10.1080/01919512.2022.2041392
Amaury de Souza, José Francisco Júnior, M. Abreu, F. Aristone, W. A. Fernandes, Raquel Soares Casaes Nunes, G. Cavazzana, Cícero Manoel dos Santos, Carlos José Reis, U. Dumka
ABSTRACT The tropospheric ozone column (O3) plays a fundamental role in terrestrial life, particularly in climate variability, and protects the terrestrial biosphere from ultraviolet (UV) radiation. The study investigates the variation of the O3 concentration column in the Pantanal based on probability distributions. The adequacy of the Log Normal (LN), GUM (Gumbel) and GEV (Generalized Extreme Values) distributions were tested using the Kolmogorov-Smirnov (KS), D (Anderson-Darling), AIC (Akaike Information Criterion), BIC (Bayesian Information Criterion), R2(coefficient of determination) and RMSE (root mean square error) tests in the period from 2005 to 2020. In most months investigated, the Pantanal follows the GUM and GEV probability distributions. In addition, monthly means of O3 and standard deviations were calculated. It is worth mentioning the variability of O3 and the impact on climate change.
{"title":"Variation of Ozone in the Pantanal Environment Based on Probability Distributions","authors":"Amaury de Souza, José Francisco Júnior, M. Abreu, F. Aristone, W. A. Fernandes, Raquel Soares Casaes Nunes, G. Cavazzana, Cícero Manoel dos Santos, Carlos José Reis, U. Dumka","doi":"10.1080/01919512.2022.2041392","DOIUrl":"https://doi.org/10.1080/01919512.2022.2041392","url":null,"abstract":"ABSTRACT The tropospheric ozone column (O3) plays a fundamental role in terrestrial life, particularly in climate variability, and protects the terrestrial biosphere from ultraviolet (UV) radiation. The study investigates the variation of the O3 concentration column in the Pantanal based on probability distributions. The adequacy of the Log Normal (LN), GUM (Gumbel) and GEV (Generalized Extreme Values) distributions were tested using the Kolmogorov-Smirnov (KS), D (Anderson-Darling), AIC (Akaike Information Criterion), BIC (Bayesian Information Criterion), R2(coefficient of determination) and RMSE (root mean square error) tests in the period from 2005 to 2020. In most months investigated, the Pantanal follows the GUM and GEV probability distributions. In addition, monthly means of O3 and standard deviations were calculated. It is worth mentioning the variability of O3 and the impact on climate change.","PeriodicalId":19580,"journal":{"name":"Ozone: Science & Engineering","volume":"46 1","pages":"130 - 146"},"PeriodicalIF":2.7,"publicationDate":"2022-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90907946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACT This study investigates modeling the ozone removal of the Reactive Black 5 (RB5) dye from an aqueous solution using a combination of nonlinear regression (NLR), multiple linear regression (MLR), and Intrinsically multiple linear regression (IMLR) models. Lack of use and evaluation of the IMLR method in estimating RB5 removal by ozonation attract attention. Experimental data were used in the R Core Team software for the development of the models and estimate of RB5 removal by ozone. The effects of variables such as pH, contact time, initial dye concentration, and applied ozone dosage on RB5 removal by ozone were investigated. Maximum 92% RB5 removal rate was obtained at pH 8, 60 min contact time, 100 mg/L initial RB5 concentration, and applied ozone dosage of 66 mgO3/L. Under these conditions, the amount of specific ozone consumption was 0.678 gO3/gRB5. In order to compare the models, coefficient of determination (R2) and mean square error (MSE) were utilized as reliability and precision criteria. The best R2 and MSE values for the IMLR model were calculated as 0.8940 and 0.098, respectively. To determine the appropriate model and coefficients, analysis of variance (ANOVA), and t-test were used, respectively. Whether the model is within the confidence interval was determined by the significance value (p) and the variation was <5% for the IMLR model. As a result, it was found that the best method for modeling RB5 removal from aqueous solution by ozone was the IMLR method. Detailed explanations on results were introduced in the study.
{"title":"Reactive Black 5 Removal with Ozone on Lab-scale and Modeling","authors":"Bulent Sari, Hakan Güney, Selman Türkeş, Olcayto Keskinkan","doi":"10.1080/01919512.2022.2035211","DOIUrl":"https://doi.org/10.1080/01919512.2022.2035211","url":null,"abstract":"ABSTRACT This study investigates modeling the ozone removal of the Reactive Black 5 (RB5) dye from an aqueous solution using a combination of nonlinear regression (NLR), multiple linear regression (MLR), and Intrinsically multiple linear regression (IMLR) models. Lack of use and evaluation of the IMLR method in estimating RB5 removal by ozonation attract attention. Experimental data were used in the R Core Team software for the development of the models and estimate of RB5 removal by ozone. The effects of variables such as pH, contact time, initial dye concentration, and applied ozone dosage on RB5 removal by ozone were investigated. Maximum 92% RB5 removal rate was obtained at pH 8, 60 min contact time, 100 mg/L initial RB5 concentration, and applied ozone dosage of 66 mgO3/L. Under these conditions, the amount of specific ozone consumption was 0.678 gO3/gRB5. In order to compare the models, coefficient of determination (R2) and mean square error (MSE) were utilized as reliability and precision criteria. The best R2 and MSE values for the IMLR model were calculated as 0.8940 and 0.098, respectively. To determine the appropriate model and coefficients, analysis of variance (ANOVA), and t-test were used, respectively. Whether the model is within the confidence interval was determined by the significance value (p) and the variation was <5% for the IMLR model. As a result, it was found that the best method for modeling RB5 removal from aqueous solution by ozone was the IMLR method. Detailed explanations on results were introduced in the study.","PeriodicalId":19580,"journal":{"name":"Ozone: Science & Engineering","volume":"5 1","pages":"50 - 64"},"PeriodicalIF":2.7,"publicationDate":"2022-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83034579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-06DOI: 10.1080/01919512.2022.2030636
K. Nakamuro, Hirotaka Naitou, Y. Nishiki, Hideo Nakata, M. Kanai, J. Hirotsuji
ABSTRACT There are many issues in the evaluation protocols based on CT (mg min/L) values, which have been used to assess the germicidal effect of highly oxidative and unstable ozonated water. The major problems include the carryover of culture medium components in virus inactivation assays and the reaction volume ratio between the virus suspension and ozonated water. Furthermore, it is essential to correct the CT value with the decay curve of dissolved ozone under the same conditions as the inactivation assay. In this study, these concerns were reexamined to obtain quantitative CT values. Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) inactivation test using ozonated water prepared from pure water was assessed by determining the corrected concentration time (CCT) values. Moreover, a possible inactivation mechanism of SARS-CoV-2 was discussed with the aid of findings from this study and previous reports. The findings revealed that the CCT value required for 99.95% inactivation of SARS-CoV-2 with ozonated water was 0.97 mg·min/L. To quantitatively evaluate the SARS-CoV-2 inactivation test, the virus purification procedure during the pretreatment and the CT value correction using a dissolved ozone decay curve obtained under the same condition as the inactivation test were demonstrated to be essential. We proposed a possible mechanism of SARS-CoV-2 inactivation with ozonated water. Amino acids such as tyrosine, tryptophan, methionine, cysteine, and histidine in the SARS-CoV-2 spike protein are susceptible to oxidative attack by the ozone dissolved in water. This attack may induce structural destruction of the spike protein and inhibit its binding to the angiotensin converting enzyme 2 (ACE2) receptor, an essential host receptor for viral infection, resulting in viral inactivation and contributing to infection suppression.
{"title":"Quantitative evaluation of the inactivation effect of ozonated water on SARS-CoV-2 based on corrected CT values","authors":"K. Nakamuro, Hirotaka Naitou, Y. Nishiki, Hideo Nakata, M. Kanai, J. Hirotsuji","doi":"10.1080/01919512.2022.2030636","DOIUrl":"https://doi.org/10.1080/01919512.2022.2030636","url":null,"abstract":"ABSTRACT There are many issues in the evaluation protocols based on CT (mg min/L) values, which have been used to assess the germicidal effect of highly oxidative and unstable ozonated water. The major problems include the carryover of culture medium components in virus inactivation assays and the reaction volume ratio between the virus suspension and ozonated water. Furthermore, it is essential to correct the CT value with the decay curve of dissolved ozone under the same conditions as the inactivation assay. In this study, these concerns were reexamined to obtain quantitative CT values. Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) inactivation test using ozonated water prepared from pure water was assessed by determining the corrected concentration time (CCT) values. Moreover, a possible inactivation mechanism of SARS-CoV-2 was discussed with the aid of findings from this study and previous reports. The findings revealed that the CCT value required for 99.95% inactivation of SARS-CoV-2 with ozonated water was 0.97 mg·min/L. To quantitatively evaluate the SARS-CoV-2 inactivation test, the virus purification procedure during the pretreatment and the CT value correction using a dissolved ozone decay curve obtained under the same condition as the inactivation test were demonstrated to be essential. We proposed a possible mechanism of SARS-CoV-2 inactivation with ozonated water. Amino acids such as tyrosine, tryptophan, methionine, cysteine, and histidine in the SARS-CoV-2 spike protein are susceptible to oxidative attack by the ozone dissolved in water. This attack may induce structural destruction of the spike protein and inhibit its binding to the angiotensin converting enzyme 2 (ACE2) receptor, an essential host receptor for viral infection, resulting in viral inactivation and contributing to infection suppression.","PeriodicalId":19580,"journal":{"name":"Ozone: Science & Engineering","volume":"21 1","pages":"148 - 156"},"PeriodicalIF":2.7,"publicationDate":"2022-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78448841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-03DOI: 10.1080/01919512.2022.2031103
G. N. Silva, J. A. Saraiva-Grossi, M. S. Carvalho, L. Faroni, Osdnéia Pereira Lopes, M. S. Oliveira, D. R. S. Barbosa, Y. Silva
ABSTRACT Macaúba (Acrocomia aculeata) is an oleaginous plant for use in biodiesel production. After harvest, the fruits of this palm tree have high water content, thus attracting microorganisms. Therefore, it is necessary to adopt appropriate fruit storage strategies to ensure oil quality. The objective of this study was to evaluate the effect of the combined use of drying and ozonation on the quality of macaúba fruit oil used to produce biodiesel. The fruits were dried at 60 °C and subsequently subjected to ozonation (treatments with different dosages and application intervals). The treatments with the combined use of drying and ozonation were efficient in maintaining FFA content below 5% for up to 45 days of storage. Furthermore, these treatments did not affect the oil content in the mesocarp of macauba fruits during 180 days of storage. Thus, the combined technique of drying and ozonation of macauba fruits without epicarps can become a viable alternative post-harvest.
{"title":"Macauba fruits preserved by combining drying and ozonation methods for biodiesel production","authors":"G. N. Silva, J. A. Saraiva-Grossi, M. S. Carvalho, L. Faroni, Osdnéia Pereira Lopes, M. S. Oliveira, D. R. S. Barbosa, Y. Silva","doi":"10.1080/01919512.2022.2031103","DOIUrl":"https://doi.org/10.1080/01919512.2022.2031103","url":null,"abstract":"ABSTRACT Macaúba (Acrocomia aculeata) is an oleaginous plant for use in biodiesel production. After harvest, the fruits of this palm tree have high water content, thus attracting microorganisms. Therefore, it is necessary to adopt appropriate fruit storage strategies to ensure oil quality. The objective of this study was to evaluate the effect of the combined use of drying and ozonation on the quality of macaúba fruit oil used to produce biodiesel. The fruits were dried at 60 °C and subsequently subjected to ozonation (treatments with different dosages and application intervals). The treatments with the combined use of drying and ozonation were efficient in maintaining FFA content below 5% for up to 45 days of storage. Furthermore, these treatments did not affect the oil content in the mesocarp of macauba fruits during 180 days of storage. Thus, the combined technique of drying and ozonation of macauba fruits without epicarps can become a viable alternative post-harvest.","PeriodicalId":19580,"journal":{"name":"Ozone: Science & Engineering","volume":"35 1","pages":"41 - 49"},"PeriodicalIF":2.7,"publicationDate":"2022-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81076215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-02DOI: 10.1080/01919512.2022.2035212
Yanfang Liu, Wei Gao, Sijie Yin, X. Lou, Gong Li, Haoyun Liu, Zaixing Li
ABSTRACT Since phosphorus (P) is a nonrenewable element essential for life, it is extremely important to understand its potential supply sources. This paper describes the behavior of P during the ozonation of waste activated sludge (WAS) as it is an important pool of P. Also, we investigated whether ozonation of WAS could be an effective method for both P recovery in solution and its bio- and plant-availability in solid residues based on the analysis by the Standards, Measurements, and Testing Program. The results showed that P was enriched in WAS at concentrations above 23 mg/g. Phosphorus transferred from the solid phase to the liquid phase, and the total P content in the liquid phase increased from 1.67 to 40.68 mg/L as the ozone dosage increased from 0 to 0.20 g O3/g MLSS. PO4 3–P was the main form of P in the liquid phase after ozonation, which is beneficial for P recovery. The P fraction analysis revealed that the released P mainly came from apatite P (AP) during ozonation. Additionally, P was more available in solid residues as the proportion of bio-available P (non-apatite inorganic P (NAIP) + organic P (OP)) improved from 73.6% in the raw WAS to 91.7% at an ozone dosage of 0.20 O3/g MLSS. In addition, the solid residues were suitable for plant availability at a moderate Fe/P ratio (0.91) after ozonation. Thus, ozonation is recommended as the preferable solution to achieve an advanced P supply in both the liquid phase and solid residues.
摘要磷是一种不可再生的生命必需元素，因此了解其潜在的供应来源至关重要。由于废活性污泥(WAS)是一个重要的磷池，本文描述了它在臭氧化过程中的行为。此外，基于标准、测量和测试程序的分析，我们研究了WAS的臭氧化是否可以成为一种有效的方法，既可以回收溶液中的磷，也可以回收固体残留物中的生物和植物可利用性。结果表明，磷在浓度大于23 mg/g时富集。随着臭氧用量从0 g O3/g MLSS增加到0.20 g O3/g MLSS，磷从固相转移到液相，液相中总磷含量从1.67 mg/L增加到40.68 mg/L。臭氧氧化后液相中磷的主要形态为PO4 - 3-P，有利于磷的回收。磷组分分析表明，臭氧氧化释放的磷主要来自磷灰石磷(AP)。此外，当臭氧用量为0.20 O3/g MLSS时，固体残渣中生物有效磷(非磷灰石无机磷(NAIP) +有机磷(OP))的比例从原was的73.6%提高到91.7%，磷在固体残渣中的可利用性更高。另外，臭氧化处理后的固体残渣在中等铁磷比(0.91)下适合植物利用。因此，臭氧氧化被推荐为在液相和固体残留物中实现高级P供应的首选解决方案。
{"title":"Behavior of Phosphorus during Sewage Sludge Ozonation","authors":"Yanfang Liu, Wei Gao, Sijie Yin, X. Lou, Gong Li, Haoyun Liu, Zaixing Li","doi":"10.1080/01919512.2022.2035212","DOIUrl":"https://doi.org/10.1080/01919512.2022.2035212","url":null,"abstract":"ABSTRACT Since phosphorus (P) is a nonrenewable element essential for life, it is extremely important to understand its potential supply sources. This paper describes the behavior of P during the ozonation of waste activated sludge (WAS) as it is an important pool of P. Also, we investigated whether ozonation of WAS could be an effective method for both P recovery in solution and its bio- and plant-availability in solid residues based on the analysis by the Standards, Measurements, and Testing Program. The results showed that P was enriched in WAS at concentrations above 23 mg/g. Phosphorus transferred from the solid phase to the liquid phase, and the total P content in the liquid phase increased from 1.67 to 40.68 mg/L as the ozone dosage increased from 0 to 0.20 g O3/g MLSS. PO4 3–P was the main form of P in the liquid phase after ozonation, which is beneficial for P recovery. The P fraction analysis revealed that the released P mainly came from apatite P (AP) during ozonation. Additionally, P was more available in solid residues as the proportion of bio-available P (non-apatite inorganic P (NAIP) + organic P (OP)) improved from 73.6% in the raw WAS to 91.7% at an ozone dosage of 0.20 O3/g MLSS. In addition, the solid residues were suitable for plant availability at a moderate Fe/P ratio (0.91) after ozonation. Thus, ozonation is recommended as the preferable solution to achieve an advanced P supply in both the liquid phase and solid residues.","PeriodicalId":19580,"journal":{"name":"Ozone: Science & Engineering","volume":"133 1","pages":"65 - 74"},"PeriodicalIF":2.7,"publicationDate":"2022-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86204261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-26DOI: 10.1080/01919512.2021.2006561
Kohei Kawaguchi, Taira Hidaka, Fumitake Nishimura
ABSTRACT 4-Chlorobenzoic acid (p-CBA) is frequently used as a hydroxyl radical (HO·) probe substance in studies of ozonation and advanced oxidation processes. However, the temperature dependence of the reaction between HO· and p-CBA remains unclear. In this context, we identified the relationship between temperature ( , K) and the apparent second-order reaction rate constant of HO· with p- CBA ( , M−1 s−1): . They were measured by a novel competitive method using 2-methylpropan-2-ol (tert-butyl alcohol) as a reference substance in the range of 1.0–40.0℃. The linear regression equation was more appropriate than the exponential regression equation to express this relationship. More generally, our simulation shows that the linear regression equation can be more accurate than the exponential regression equation to express the relationship between temperature and apparent reaction rate constants of HO.
{"title":"Linear Relationship between Temperature and the Apparent Reaction Rate Constant of Hydroxyl Radical with 4-chlorobenzoic Acid","authors":"Kohei Kawaguchi, Taira Hidaka, Fumitake Nishimura","doi":"10.1080/01919512.2021.2006561","DOIUrl":"https://doi.org/10.1080/01919512.2021.2006561","url":null,"abstract":"ABSTRACT 4-Chlorobenzoic acid (p-CBA) is frequently used as a hydroxyl radical (HO·) probe substance in studies of ozonation and advanced oxidation processes. However, the temperature dependence of the reaction between HO· and p-CBA remains unclear. In this context, we identified the relationship between temperature ( , K) and the apparent second-order reaction rate constant of HO· with p- CBA ( , M−1 s−1): . They were measured by a novel competitive method using 2-methylpropan-2-ol (tert-butyl alcohol) as a reference substance in the range of 1.0–40.0℃. The linear regression equation was more appropriate than the exponential regression equation to express this relationship. More generally, our simulation shows that the linear regression equation can be more accurate than the exponential regression equation to express the relationship between temperature and apparent reaction rate constants of HO.","PeriodicalId":19580,"journal":{"name":"Ozone: Science & Engineering","volume":"19 1","pages":"274 - 280"},"PeriodicalIF":2.7,"publicationDate":"2022-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82921603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-10DOI: 10.1080/01919512.2021.2021849
Vera Lúcia Taba, H. C. Carvalho, Túlia de Souza Botelho, A. B. Fernandes, Carlos José de Lima, A. Villaverde
ABSTRACT Ethylene oxide and gamma irradiation are two well-established sterilizing processes for surgical materials; however, they present toxicity and high cost, respectively. Ozone Gas, O3(g), has been used effectively for disinfection and sterilization. Low pressure (LP) can promote water vaporization whenever the vapor pressure drops below the water saturation pressure, which at 20 °C (experimental temperature) is 2337 Pa. The microorganism used to contaminate the surgical needles (SN) was Staphylococcus aureus, a gram-positive bacterium whose cytoplasm is mostly water. Sixty SN samples were divided into six groups of 10: negative control (sterile SN), positive control (experimentally contaminated SN), LP group, two groups associating LP and O3(g), and O3(g) alone group. The CFU/mL count demonstrated microbicide efficacy of LP+O3(g) and O3(g) groups. This is an innovative protocol for microbial inactivation, mainly because of LP application. In the future, this technique can be evaluated for sterilization of SN and other medical devices.
{"title":"Inactivation of Staphylococcus aureus in Surgical Needles by Exposure to Ozone Gas and Low Pressure","authors":"Vera Lúcia Taba, H. C. Carvalho, Túlia de Souza Botelho, A. B. Fernandes, Carlos José de Lima, A. Villaverde","doi":"10.1080/01919512.2021.2021849","DOIUrl":"https://doi.org/10.1080/01919512.2021.2021849","url":null,"abstract":"ABSTRACT Ethylene oxide and gamma irradiation are two well-established sterilizing processes for surgical materials; however, they present toxicity and high cost, respectively. Ozone Gas, O3(g), has been used effectively for disinfection and sterilization. Low pressure (LP) can promote water vaporization whenever the vapor pressure drops below the water saturation pressure, which at 20 °C (experimental temperature) is 2337 Pa. The microorganism used to contaminate the surgical needles (SN) was Staphylococcus aureus, a gram-positive bacterium whose cytoplasm is mostly water. Sixty SN samples were divided into six groups of 10: negative control (sterile SN), positive control (experimentally contaminated SN), LP group, two groups associating LP and O3(g), and O3(g) alone group. The CFU/mL count demonstrated microbicide efficacy of LP+O3(g) and O3(g) groups. This is an innovative protocol for microbial inactivation, mainly because of LP application. In the future, this technique can be evaluated for sterilization of SN and other medical devices.","PeriodicalId":19580,"journal":{"name":"Ozone: Science & Engineering","volume":"21 1","pages":"19 - 27"},"PeriodicalIF":2.7,"publicationDate":"2022-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83909150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-06DOI: 10.1080/01919512.2021.2022452
Sílvia Móbille Awoyama, H. C. Carvalho, Túlia de Souza Botelho, Sandra Irene Sprogis dos Santos, Debora Alicia Buendía Palacios, Sebastian San Martín Henríque, R. Zângaro, Carlos José de Lima, A. B. Fernandes
ABSTRACT Human amniotic membrane (hAM) has a great potential in regenerative medicine as it can be disinfected, preserved, and stored. The present study evaluated a hydrodynamic system that uses ozonated water for disinfecting hAM and assessed possible morphological alterations using histological analysis and scanning electron microscopy (SEM). hAM fragments were experimentally contaminated with Staphylococcus aureus (103 CFU/mL) and submitted to disinfection processes for 5, 10 and 15 minutes (1.95, 3.9 and 5.85 mg/cm2 (O3), respectively). The results of the water microbiological analysis showed that no microbial growth was detected in all water samples. The hAM surface monitoring with swab method and the analysis of fragment inoculated directly into the culture media revealed that no microbial growth was detected after 10 and 15 minutes. The SEM and histological analysis of hAM revealed that morphological alterations in the epithelium began after 10 minutes 3.9 mg/cm2 (O3) of the disinfection process, and significant changes in the epithelial cells were observed after 15 minutes (5.85 mg/cm2 (O3)). These findings indicates that the structural integrity of hAM was maintained during the process and suggest that the technique is a promising system to disinfect this biological material.
{"title":"Disinfection of Human Amniotic Membrane Using a Hydrodynamic System with Ozonated Water","authors":"Sílvia Móbille Awoyama, H. C. Carvalho, Túlia de Souza Botelho, Sandra Irene Sprogis dos Santos, Debora Alicia Buendía Palacios, Sebastian San Martín Henríque, R. Zângaro, Carlos José de Lima, A. B. Fernandes","doi":"10.1080/01919512.2021.2022452","DOIUrl":"https://doi.org/10.1080/01919512.2021.2022452","url":null,"abstract":"ABSTRACT Human amniotic membrane (hAM) has a great potential in regenerative medicine as it can be disinfected, preserved, and stored. The present study evaluated a hydrodynamic system that uses ozonated water for disinfecting hAM and assessed possible morphological alterations using histological analysis and scanning electron microscopy (SEM). hAM fragments were experimentally contaminated with Staphylococcus aureus (103 CFU/mL) and submitted to disinfection processes for 5, 10 and 15 minutes (1.95, 3.9 and 5.85 mg/cm2 (O3), respectively). The results of the water microbiological analysis showed that no microbial growth was detected in all water samples. The hAM surface monitoring with swab method and the analysis of fragment inoculated directly into the culture media revealed that no microbial growth was detected after 10 and 15 minutes. The SEM and histological analysis of hAM revealed that morphological alterations in the epithelium began after 10 minutes 3.9 mg/cm2 (O3) of the disinfection process, and significant changes in the epithelial cells were observed after 15 minutes (5.85 mg/cm2 (O3)). These findings indicates that the structural integrity of hAM was maintained during the process and suggest that the technique is a promising system to disinfect this biological material.","PeriodicalId":19580,"journal":{"name":"Ozone: Science & Engineering","volume":"2 1","pages":"28 - 40"},"PeriodicalIF":2.7,"publicationDate":"2022-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83694220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-03DOI: 10.1080/01919512.2021.2004877
Dalanda Hamdi, Lobna Mansouri, Yu-In Park, V. Srivastava, Mika Sillanpaa, L. Bousselmi
ABSTRACT A continuous system for photo-catalytic/ozonation was designed at laboratory scale and evaluated for water treatment using Amido Black 10B dye (AB) as a pollutant model. The effect of single ozonation (O3), photolytic ozonation (UV/O3) and combined photocatalytic/ozonation treatment (UV/O3/TiO2) were investigated. TiO2 nanocatalysts films were coated on stainless steel grids (AISI 304) by Atomic Layer Deposition technique (ALD) at 350 °C. An experimental hydrodynamic study of the designed reactor was conducted based on dry and total pressure drop. Three flow regimes were observed by increasing the total pressure drop against gas load factor (Fs). Depollution experiments conducted on the developed reactor showed that reaction rates and efficiency of studied processes were dependent on the studied operational parameters (initial dye concentration, ozone dose, pH). The maximum AB dye ([AB]0 = 100 mg. L−1) conversion efficiency, using the designed (UV/O3/TiO2) system, was about 40.3% of initial TOC and 100% AB color removal was achieved within 10 min at an optimal ozone dose of 132 mg. L−1. A significant improvement in AB removal using UV/TiO2/O3 process (koverall = 0.056 min−1, 40.3%) compared to O3 alone (koverall = 0.032 min−1, 26.87). Results shows also that the addition of tert-butanol (TB), as radical scavenger ([TB] = 0.2 M and 0.4 M), greatly affects the reaction mechanisms of a photocatalytic ozonation process within 5 min of reaction, by decreasing the Amido Black conversion from 99.67% ([TB] = 0 M) to 74.63% and 31.35%, respectively, in presence of 0.2 M and 0.4 M of tert-butanol. The remarkable decrease in AB removal rate observed in the presence of a radical scavenger expresses that there was an obvious enhancement of hydroxyl radical OH• generation due to the synergetic effect between ozone and photocatalytic processes. Graphical abstract
{"title":"Development of a Continuous Photo-catalytic/Ozonation System: Application on Amido Black Removal from Water","authors":"Dalanda Hamdi, Lobna Mansouri, Yu-In Park, V. Srivastava, Mika Sillanpaa, L. Bousselmi","doi":"10.1080/01919512.2021.2004877","DOIUrl":"https://doi.org/10.1080/01919512.2021.2004877","url":null,"abstract":"ABSTRACT A continuous system for photo-catalytic/ozonation was designed at laboratory scale and evaluated for water treatment using Amido Black 10B dye (AB) as a pollutant model. The effect of single ozonation (O3), photolytic ozonation (UV/O3) and combined photocatalytic/ozonation treatment (UV/O3/TiO2) were investigated. TiO2 nanocatalysts films were coated on stainless steel grids (AISI 304) by Atomic Layer Deposition technique (ALD) at 350 °C. An experimental hydrodynamic study of the designed reactor was conducted based on dry and total pressure drop. Three flow regimes were observed by increasing the total pressure drop against gas load factor (Fs). Depollution experiments conducted on the developed reactor showed that reaction rates and efficiency of studied processes were dependent on the studied operational parameters (initial dye concentration, ozone dose, pH). The maximum AB dye ([AB]0 = 100 mg. L−1) conversion efficiency, using the designed (UV/O3/TiO2) system, was about 40.3% of initial TOC and 100% AB color removal was achieved within 10 min at an optimal ozone dose of 132 mg. L−1. A significant improvement in AB removal using UV/TiO2/O3 process (koverall = 0.056 min−1, 40.3%) compared to O3 alone (koverall = 0.032 min−1, 26.87). Results shows also that the addition of tert-butanol (TB), as radical scavenger ([TB] = 0.2 M and 0.4 M), greatly affects the reaction mechanisms of a photocatalytic ozonation process within 5 min of reaction, by decreasing the Amido Black conversion from 99.67% ([TB] = 0 M) to 74.63% and 31.35%, respectively, in presence of 0.2 M and 0.4 M of tert-butanol. The remarkable decrease in AB removal rate observed in the presence of a radical scavenger expresses that there was an obvious enhancement of hydroxyl radical OH• generation due to the synergetic effect between ozone and photocatalytic processes. Graphical abstract","PeriodicalId":19580,"journal":{"name":"Ozone: Science & Engineering","volume":"1 1","pages":"545 - 565"},"PeriodicalIF":2.7,"publicationDate":"2022-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89775365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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