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Ozonation of Bovine Peritoneal Membrane for Preservation: Preliminary Investigation 臭氧氧化保存牛腹膜的初步研究
IF 2.7 4区 环境科学与生态学 Q3 ENGINEERING, ENVIRONMENTAL Pub Date : 2021-09-26 DOI: 10.1080/01919512.2021.1984205
L. H. Moreira, M. K. Salomão, H. C. Carvalho, André Luis Silva Mendes, R. A. Zângaro, A. B. Fernandes, Carlos José de Lima
ABSTRACT A dynamic fluid system using ozonated water flowing over the bovine peritoneal membrane was developed. Histological analyses were performed to verify the maximum time of ozone exposure to maintain the viability of the biological material. The results indicate that the biological viability of the peritoneum tissue was impaired after 15 minutes of exposure (5.5 mg O3 (cm2)−1) to ozonated water in a dynamic condition. Thus, this biological sample can be submitted to the dynamic fluid system with ozonated water for 10 minutes at an applied ozone dose of 3.7 mg O3 (cm2)−1, without inducing significant changes to the tissue.
建立了一种利用臭氧化水在牛腹膜上流动的动态流体系统。进行了组织学分析,以验证臭氧暴露的最长时间,以维持生物材料的活力。结果表明,在动态条件下(5.5 mg O3 (cm2)−1)暴露于臭氧化水15分钟后,腹膜组织的生物活力受损。因此,该生物样品可以在3.7 mg O3 (cm2)−1的臭氧剂量下,在含臭氧水的动态流体系统中放置10分钟,而不会引起组织的显著变化。
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引用次数: 3
Intermittent Root Flushing with Ozonated Water Promotes Growth of Japanese Mustard Spinach (Brassica rapa var. perviridis) grown in a Nutrient Film Technique Hydroponic Culture—Preliminary Results 臭氧水间歇根冲洗促进营养膜技术水培培养的日本芥菜菠菜(Brassica rapa var. perviridis)的生长-初步结果
IF 2.7 4区 环境科学与生态学 Q3 ENGINEERING, ENVIRONMENTAL Pub Date : 2021-09-07 DOI: 10.1080/01919512.2021.1967723
M. Ishii, Vu Phong Lam, K. Fujiwara, J. Park
ABSTRACT Japanese mustard spinach with 4–5 unfolded leaves were transplanted in a nutrient film technique hydroponic system to investigate the effects of washing roots intermittently with ozonated water (OW) on plant growth. The high concentration of OW generated electrolytically was mixed in the nutrient solution tank, showed 5.8 mg.L−1, and circulated through the culture beds for 1 h every week. After washing the roots with OW or tap water (TW) as a control for 1 h, Otsuka house A solution (NO3-N: 16.8 me.L−1, NH4-N: 1.8 me.L−1, P: 5.1 me.L−1, K: 8.6 me.L−1, Ca: 8.2 me.L−1, Mg: 3.0 me.L−1) at 1.2 dS.m–1 was provided for plant cultivation in hydroponic culture systems. The OW or TW was replaced every week. The fresh and dry weights of the leaves, number of leaves, leaf length, and leaf width of plants treated with OW were significantly higher than those of the plants treated with TW at 1, 2, and 3 weeks after transplanting (WAT). The ratio of the shoot and root dry weights at 1, 2, and 3 WAT was significantly higher in plants grown in the OW flushing treatment compared with the control treatment. However, the roots dry weights were not significantly different between the OW- and TW-treated plants. Moreover, the number of coliform bacteria and aerobic plate count (APC) in the solution and plants were not significantly different between the two treatments. These results indicate that washing roots intermittently with 5.8 mg.L−1 dissolved ozone concentration of OW for 1 h per week promotes aerial plant growth without physiological disorder and is not accompanied by a decrease in the number of coliform bacteria and APC in the solution and plants.
摘要采用营养膜技术水培系统,研究臭氧水间歇洗根对4 ~ 5片未展开叶片的芥菜菠菜生长的影响。在营养液槽中混合电解生成的高浓度OW,为5.8 mg。L−1,每周在培养床上循环1h。在用OW或自来水(TW)作为对照洗根1小时后,大冢将一种溶液(NO3-N: 16.8 me)放入室内。L−1,NH4-N: 1.8 me。L−1,P: 5.1 m。L−1,K: 8.6 me。L−1,Ca: 8.2 me。L−1,Mg: 3.0 .L−1)在1.2 dS。M-1用于水培栽培系统。OW或TW每周更换一次。移栽后1、2、3周,OW处理植株的鲜重、干重、叶数、叶长、叶宽均显著高于TW处理植株。在1、2、3个WAT时,OW冲洗处理植株的茎、根干重比显著高于对照处理。而根系干重在OW和tw处理间差异不显著。此外,两种处理间溶液和植株中大肠菌群数量和需氧平板计数(APC)无显著差异。这些结果表明,间歇洗根5.8 mg。L−1溶解臭氧浓度的OW每周处理1 h,可促进地上植物生长,无生理障碍,且不伴有溶液和植物中大肠菌群和APC数量的减少。
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引用次数: 3
Antifungal Action of Ozone on Chicken Eggshell Cuticles: A Preliminary Study 臭氧对鸡蛋壳表皮抑菌作用的初步研究
IF 2.7 4区 环境科学与生态学 Q3 ENGINEERING, ENVIRONMENTAL Pub Date : 2021-08-24 DOI: 10.1080/01919512.2021.1967722
C. Soares, Cláudio Eduardo Cartabiano Leite, F. Dahlke, A. Maiorka, Marília Miotto, V. Scussel, J. De Dea Lindner
ABSTRACT Ozone gas (O3) is used as an oxidizing agent and can be applied as a sanitizer to control microorganisms and degrade toxic compounds, such as pesticides and mycotoxins, in food industries. Some studies investigated the use of O3 as an agent to reduce pathogenic bacteria such as Salmonella enteritidis. The action of O3 on the eggshell cuticle and structures of filamentous fungi is still not yet well studied. In this work, scanning electron microscopy (SEM) was used to assess the hens’ eggshell structure after the exposition to O3, mainly to obtain information about the potential antifungal action. The SEM micrographs obtained shown that the application of O3 during 120 min damaged fungi hyphae adhered to the eggshell cuticle without severely compromising the external structure of the eggshell. Further studies are necessary to establish an ideal O3 treatment for the fungal decontamination of chicken eggs.
臭氧气体(O3)在食品工业中被用作氧化剂和消毒剂,以控制微生物和降解有毒化合物,如农药和真菌毒素。一些研究调查了使用臭氧作为减少肠炎沙门氏菌等致病菌的药剂。O3对丝状真菌蛋壳角质层和结构的作用尚未得到很好的研究。本研究采用扫描电镜(SEM)对O3处理后母鸡的蛋壳结构进行了研究,主要是为了了解O3潜在的抗真菌作用。SEM显微图显示,O3在120 min的时间内破坏了真菌菌丝粘附在蛋壳角质层上,但没有严重损害蛋壳的外部结构。需要进一步的研究来确定理想的O3处理方法来去除鸡蛋的真菌污染。
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引用次数: 2
Degradation Kinetics Study of Fast Ozonation Reaction in a Rotating Packed Bed 旋转填料床快速臭氧化反应降解动力学研究
IF 2.7 4区 环境科学与生态学 Q3 ENGINEERING, ENVIRONMENTAL Pub Date : 2021-08-22 DOI: 10.1080/01919512.2021.1967724
Dan-qiao Wang, Taoran Liu, Lei Ma, Feng Wang, Jimmy Yun, L. Shao
ABSTRACT The degradation kinetics of different organic compounds in a rotating packed bed (RPB) were investigated in this work. Phenol, catechol, hydroquinone, and aniline that can react rapidly with ozone were chosen to be the model pollutants, and the lumped parameter models were established to characterize the reaction rate of fast ozonation of those organic compounds in the RPB. Correlations for the lumped parameters of the organic compounds degradation (A 1) and COD removal (A 2) were obtained by fitting the experimental data. The deviations between experimental and predicted A 1 and A 2 were generally within 25%, indicating that the ozonation rate model has a good predictability for fast ozonation between ozone and organic compounds in the RPB.
研究了不同有机化合物在旋转填料床(RPB)中的降解动力学。选择苯酚、儿茶酚、对苯二酚和苯胺等能与臭氧快速反应的污染物作为模型污染物,建立了集总参数模型,表征了这些有机化合物在RPB中快速臭氧化的反应速率。通过对实验数据的拟合,得到了有机化合物降解(a1)和COD去除率(a2)的集总参数的相关性。实验a1和a2与预测值的偏差一般在25%以内,表明臭氧化速率模型对RPB中臭氧与有机物之间的快速臭氧化具有较好的可预测性。
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引用次数: 2
Intensification of Ozone Mass Transfer and Generation of Hydroxyl Radicals by Ceramic Membrane 陶瓷膜强化臭氧传质及羟基自由基的生成
IF 2.7 4区 环境科学与生态学 Q3 ENGINEERING, ENVIRONMENTAL Pub Date : 2021-08-09 DOI: 10.1080/01919512.2021.1960149
Shu Zhu, G. Cheng, X. Quan, Facheng Qiu, Weiyang Bai, Yijuan Tian
ABSTRACT The strengthening ability of ozone to treat the reverse osmosis concentrate (ROC) of landfill leachate is a crucial issue. From the standpoint of coupling intensification, a ceramic membrane/ozone (CM/O3) system was developed to improve the oxidation performance of ozone. The results showed that CM/O3 system could significantly improve the ozone concentration and the generation of hydroxyl radicals (·OH) when compared to the traditional O3 bubble system. The reaction condition, such as inlet ozone concentration and ozone flow, ceramic membrane pore size, transmembrane pressure, and temperature on the concentration of ozone in the liquid phase and the formation of ·OH, has been considered. Experimental results showed that the concentration of ozone and OH in the liquid increased with the increase in the inlet ozone concentration and ozone flow. Moreover, a certain degree of reduction in membrane pores was facilitated to intensify the mass transfer of ozone. The increase of pressure was able to raise the concentration of ozone. However, there was a slight effect on ·OH concentration. As for the temperature, a low-temperature situation was conducive to strengthen ozone dissolution. In terms of the generation of ·OH, a high-temperature situation was a better favorable environment. In order to evaluate the performance of the reactor system, three treatment processes (CM/O3, CM alone and O3 alone) were compared to treat ROC. In the CM/O3 system, the final removal efficiency of Chemical Oxygen Demand (COD) was 81.9%, and the final removal efficiency of Total Organic Carbon (TOC) was 62.6%.
臭氧对垃圾渗滤液反渗透浓缩液(ROC)的强化处理能力是一个关键问题。从耦合强化的角度出发,开发了陶瓷膜/臭氧(CM/O3)体系,以提高臭氧的氧化性能。结果表明,与传统的O3气泡体系相比,CM/O3体系能显著提高臭氧浓度和羟基自由基(·OH)的生成。考虑了进口臭氧浓度和臭氧流量、陶瓷膜孔径、跨膜压力、温度等反应条件对液相臭氧浓度和·OH生成的影响。实验结果表明,随着进口臭氧浓度和臭氧流量的增加,液体中臭氧和OH的浓度增加。此外,膜孔的一定程度的减少有助于加强臭氧的传质。压力的增加能够提高臭氧的浓度。而对·OH浓度的影响较小。在温度方面,低温有利于加强臭氧的溶解。就生成·OH而言,高温条件是较有利的环境。为了评价反应器系统的性能,比较了三种处理工艺(CM/O3、CM单独和O3单独)处理ROC。在CM/O3体系中,化学需氧量(COD)的最终去除率为81.9%,总有机碳(TOC)的最终去除率为62.6%。
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引用次数: 1
Effect of Magnetic Field on Multi-parameters of Needle Plate DBD Ozone Generator 磁场对针板式DBD臭氧发生器多参数的影响
IF 2.7 4区 环境科学与生态学 Q3 ENGINEERING, ENVIRONMENTAL Pub Date : 2021-08-04 DOI: 10.1080/01919512.2021.1960146
Shukai Sun, Ying Chen, P. Yuan, Longsheng Xu, Yafang Zhang, Linsheng Wei
ABSTRACT The oxidation of ozone is very strong, and the products of oxidative decomposition will not cause secondary pollution, so the ozone has become widely used. Dielectric barrier discharge (DBD) is the main method of ozone generation. In recent years, the addition of outfield in the DBD is used to improve ozone generation. In this paper, a needle-plate DBD ozone generator with magnetic field enhanced was designed and manufactured. The authors found the magnetic field could significantly increase the number and amplitude of current pulses, discharge current and the discharge power. The magnetic field did not change the initial discharge voltage. With the discharge voltage increased, the enhancement of magnetic field on ozone concentration was more obvious while on ozone yield was very small. The maximum increment of ozone concentration was 35.4%. With the discharge frequency increased, the magnetic field had a more positive impact on the ozone concentration. And with the same gas flow rate increased, the ozone concentration was increased by the magnetic field. Keeping the SIE constant, the ozone concentration and ozone yield at higher discharge frequency was lower than those at lower discharge frequency. With the increase of SIE, the enhancement effect of magnetic field on ozone concentration would increase.
臭氧的氧化性很强,氧化分解后的产物不会造成二次污染,因此臭氧得到了广泛的应用。介质阻挡放电(DBD)是臭氧生成的主要方法。近年来,在DBD中增加外场是为了改善臭氧的产生。本文设计并制造了一种磁场增强针板式DBD臭氧发生器。研究发现,磁场能显著增加电流脉冲数和幅值,增加放电电流和放电功率。磁场没有改变初始放电电压。随着放电电压的增加,磁场对臭氧浓度的增强更明显,而对臭氧产率的影响很小。臭氧浓度最大增幅为35.4%。随着放电频率的增加,磁场对臭氧浓度的影响越显著。在相同气体流速增大的情况下,磁场对臭氧浓度的影响增大。在SIE一定的条件下,高频率放电时臭氧浓度和臭氧产率均低于低频率放电时。随着SIE的增大,磁场对臭氧浓度的增强作用增强。
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引用次数: 0
Detection of Cryptosporidium Parvum Oocysts in Artificially Contaminated Filter Backwash Water and Ozone Treatment at Pilot Scale 人工污染滤池反冲洗水中细小隐孢子虫卵囊的检测及臭氧中试处理
IF 2.7 4区 环境科学与生态学 Q3 ENGINEERING, ENVIRONMENTAL Pub Date : 2021-08-02 DOI: 10.1080/01919512.2021.1960148
A. P. Ogura, L. P. Sabogal-Paz
ABSTRACT Waterborne diseases are a relevant concern for public health systems since commonly applied treatment techniques may not remove all water contaminants. Cryptosporidium spp. oocysts are an issue in water treatment plants due to their reduced size and resistance to the disinfection process (e.g., chlorination). Moreover, oocysts retained on the filter media might recirculate amongst the filter backwash water (FBW). This study aimed to detect Cryptosporidium parvum oocysts artificially inoculated on the FBW and evaluate the ozone treatment performance. A synthetic FBW underwent three concentration methods followed by immunomagnetic separation: calcium carbonate flocculation, direct centrifugation, and direct centrifugation with the 7X ICN dispersion solution. The latter method was selected as it presented less interference on oocyst viability (37.2% reduction) and higher recovery (22.1%) on preliminary assays. The recovery for the commercial suspensions was 15.4 ± 3.3%, although the analytical quality performed with EasySeed® suspension obtained a recovery of 2.8 ± 0.8%. These limitations and methodologies for protozoan detection are challenging due to low recoveries, especially in complex matrices. Finally, FBW ozonation was performed on a pilot scale, and the propidium iodide dye indicated oocyst viability decreased after treatment. Oocyst inactivation was 2.83 log and 3.44 log for dosages of 7.5 mg O3 L−1 for 10 min (i.e., 75 mg min L−1) and 10 mg O3 L−1 for 5 min (i.e., 50 mg min L−1), respectively. Disinfection is a crucial pathway for addressing outbreak scenarios, and ozone treatment should be further studied. Graphical abstract
水传播疾病是公共卫生系统的一个相关问题,因为通常应用的处理技术可能无法去除所有的水污染物。隐孢子虫卵囊是水处理厂的一个问题,因为它们体积小,对消毒过程(如氯化)有抵抗力。此外,保留在过滤介质上的卵囊可能在过滤器反冲洗水中再循环。本研究旨在检测人工接种的小隐孢子虫卵囊,并评价臭氧处理效果。采用碳酸钙絮凝法、直接离心法和7X ICN分散液直接离心法三种浓缩后的免疫磁分离方法制备合成的FBW。选择后一种方法,是因为其对卵囊活力的干扰较小(降低37.2%),初步测定回收率较高(22.1%)。商用混悬液的回收率为15.4±3.3%,而EasySeed®混悬液的分析质量为2.8±0.8%。由于回收率低,特别是在复杂的基质中,原生动物检测的这些限制和方法具有挑战性。最后,在中试规模上进行了FBW臭氧化,碘化丙啶染料显示处理后卵囊活力下降。7.5 mg o3l - 1作用10分钟(即75 mg min L - 1)和10 mg o3l - 1作用5分钟(即50 mg min L - 1)时,卵囊失活率分别为2.83 log和3.44 log。消毒是解决疫情的关键途径,臭氧处理应进一步研究。图形抽象
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引用次数: 2
Inactivation of Multidrug-Resistant Salmonella Heidelberg Isolated from Pigs by Ozonated Water under Different Conditions 不同条件下臭氧水对猪源多重耐药海德堡沙门氏菌的灭活研究
IF 2.7 4区 环境科学与生态学 Q3 ENGINEERING, ENVIRONMENTAL Pub Date : 2021-08-01 DOI: 10.1080/01919512.2021.1960147
Eduardo de Paula Nascente, Úrsula Nunes Rauecker, A. V. Teles, Lorena Dias do Amor Divino, Sarah Rodrigues Chagas, Luan Mendes Elias, M. P. Matos, M. A. Andrade, L. M. Pascoal
ABSTRACT Salmonella Heidelberg is frequently associated with diseases transmitted by food of animal origin, so that the cleaning and disinfection of pigs farms are important for the control of this pathogen. This research was aimed to test the biocidal potential of ozonated water in different concentrations for the inactivation of a Salmonella Heidelberg isolated from pigs. We analyzed the strain antibiotic sensitivity profile to 17 antimicrobials and exposed the strain to disinfectant and three different concentrations of ozonated water (0.5 mg/L, 2.4 mg/L, and 4.4 mg/L) for one, five, and 10 minutes, in presence and absence of organic matter (OM), and performed a quantitative assessment of the bacterial genomic DNA. Salmonella Heidelberg was characterized as multidrug-resistant. Ozonated water inhibited bacterial growth in the absence of OM after one minute, with changes in bacterial DNA concentration.
摘要海德堡沙门氏菌常与动物源性食品传播的疾病有关,因此猪场的清洁和消毒对控制这种病原体非常重要。本研究旨在测试不同浓度臭氧化水对猪分离的海德堡沙门氏菌的灭活能力。我们分析了菌株对17种抗菌素的抗生素敏感性,并将菌株暴露于消毒剂和三种不同浓度的臭氧水(0.5 mg/L, 2.4 mg/L和4.4 mg/L)中1分钟,5分钟和10分钟,存在和不存在有机物(OM),并对细菌基因组DNA进行定量评估。海德堡沙门氏菌具有多重耐药特征。在没有OM的情况下,臭氧水在1分钟后抑制细菌生长,细菌DNA浓度发生变化。
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引用次数: 2
Ozone: An Advanced Oxidation Technology to Enhance Sustainable Food Consumption through Mycotoxin Degradation 臭氧:通过真菌毒素降解促进可持续食品消费的先进氧化技术
IF 2.7 4区 环境科学与生态学 Q3 ENGINEERING, ENVIRONMENTAL Pub Date : 2021-07-15 DOI: 10.1080/01919512.2021.1948388
O. J. Sujayasree, A. Chaitanya, R. Bhoite, R. Pandiselvam, Anjineyulu Kothakota, Mohsen Gavahian, A. Khaneghah
ABSTRACT Mycotoxins are health-threatening fungal metabolites that have been found in several foods around the world. Although agricultural, transportation, and storage management strategies have been employed to reduce the production of mycotoxins, they are not effective in eliminating mycotoxins. In this context, the application of ozone has emerged for the degradation of mycotoxins. Ozone has a strong oxidation rate and generates more free radicals, which can counter the functional groups of the mycotoxin by changing their molecular structures and forming products having lower molecular weight reduced number of double bonds, and reduced toxicity. Research indicates that ozone could be able to destroy mycotoxins without leaving any residues in the commodities. The ozone processing parameters, surface and nutritional properties of food, and fungal species are the primary determinants affecting the processing efficacy. Ozone can contribute to sustainable food consumption through mycotoxin degradation to achieve sustainable development goals (SDGs).
真菌毒素是一种威胁健康的真菌代谢物,已在世界各地的几种食物中发现。尽管已采用农业、运输和储存管理策略来减少真菌毒素的产生,但它们在消除真菌毒素方面并不有效。在这种情况下,臭氧在真菌毒素降解方面的应用已经出现。臭氧具有较强的氧化速率,产生较多的自由基,可以通过改变霉菌毒素的分子结构来对抗其官能团,形成分子量更低、双键数减少、毒性降低的产物。研究表明,臭氧可以消灭真菌毒素,而不会在商品中留下任何残留物。臭氧处理参数、食品表面和营养特性以及真菌种类是影响臭氧处理效果的主要决定因素。臭氧可以通过真菌毒素降解促进可持续粮食消费,从而实现可持续发展目标。
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引用次数: 29
Effects of High Concentration Ozone Gas Fumigation on the Quality and Shelf-life of Longan Fruit 高浓度臭氧气体熏蒸对桂圆果实品质和保质期的影响
IF 2.7 4区 环境科学与生态学 Q3 ENGINEERING, ENVIRONMENTAL Pub Date : 2021-07-07 DOI: 10.1080/01919512.2021.1948387
Saranyapak Chamnan, J. Varith, S. Jaturonglumlert, J. Phimphimol, Narathip Sujinda
ABSTRACT The objective of this research was to study the effects of high concentration ozone (HCO) fumigation on the shelf-life of fresh longan. Longan was exposed to ozone gas at varying inlet concentrations of 4,000, 8,500, and 13,000 ppm with holding times of 5 and 15 min. Results showed that the longan exposed to ozone gas at 8,500 ppm for 5 min was considered a suitable treatment to extend shelf-life up to 35 days, which is 57% longer than the shelf-life of non-ozonated longan. The SEM revealed that the ozonated longan had less surface epidermal hairs than the non-ozonated longan.
摘要本研究旨在研究高浓度臭氧(HCO)熏蒸对鲜龙眼保质期的影响。将龙眼分别暴露于进口浓度为4000、8500和13000 ppm的臭氧气体中,保温时间分别为5和15 min。结果表明,臭氧浓度为8500 ppm的龙眼保温时间为5 min,可将龙眼的保质期延长至35天,比未臭氧化的龙眼保质期延长57%。扫描电镜显示,臭氧化后的龙眼表皮毛比未臭氧化的龙眼少。
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引用次数: 3
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Ozone: Science & Engineering
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