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Bioactive compounds, Antioxidant and Antimicrobial Attributes of Cordia Sinensis Lam 冬虫夏草的生物活性化合物、抗氧化和抗菌特性
Pub Date : 2024-05-03 DOI: 10.38211/joarps.2024.05.239
Asghar Ali Shaikh, S. H. Naqvi, Komal Siddiqui, F. Korejo, Zameer Ali Palh, Abdul Sami Dahri, Muhammad Rafiq, Shayan Ali
The aim of this study is to discover the efficacy of various parts of Cordia sinensis plant against some particular pathogens that are recognized to cause diseases and to check antioxidant and bioactive compounds from different parts of plant C. sinensis. Plant extracts were examined by quantification of phytochemical compounds and antimicrobial activity. Phytochemicals and several secondary metabolites were quantified and also qualitatively analyzed in C. sinensis extracts such as phenolic compounds, flavonoids, alkaloids, tannins, steroids, glycosides, and saponins. Similarly, biochemical primary metabolites like protein, total sugar, and reducing sugar were also estimated in different parts of C. sinensis and these results correlated with antimicrobial activity. This study reveals that acetone extracts of stems, leaves, and roots of the C. sinensis showed excellent antibacterial activity against Staphylococcus aureus, Pseudomonas aeruginosa, and Klebsiella pneumonia and also against fungal species Aspergillus niger, Candida albicans, and Penicillium notatum.
本研究的目的是发现冬虫夏草不同部位对一些公认的致病病原体的功效,并检查冬虫夏草不同部位的抗氧化剂和生物活性化合物。对植物提取物进行了植物化学化合物定量和抗菌活性检测。对中华皂苷提取物中的植物化学物质和一些次生代谢物进行了定量和定性分析,如酚类化合物、黄酮类化合物、生物碱、单宁、甾体、苷类和皂苷。同样,还估算了中华皂苷不同部位的生化初级代谢产物,如蛋白质、总糖和还原糖,这些结果与抗菌活性相关。这项研究表明,中华皂草的茎、叶和根的丙酮提取物对金黄色葡萄球菌、铜绿假单胞菌和肺炎克雷伯氏菌以及真菌黑曲霉、白色念珠菌和烟青霉菌具有很好的抗菌活性。
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引用次数: 0
Combining Ability Analysis and Genetic Studies of Stripe Rust Resistance in Bread Wheat Genotypes 面包小麦基因型条锈病抗性的综合能力分析和遗传研究
Pub Date : 2024-03-30 DOI: 10.38211/joarps.2024.05.237
Mir Yar Mohammad Talpur, A. W. Baloch, M. Baloch, M. Asad
A set of eighteen F1 and F2 experimental crosses were grown in a randomized complete block design (RCBD) with three replications. From lines, TD-1 was on top by showing greater and significant general combining ability (GCA) effects for maximum number characters including grain yield in both (F1 and F2) populations, while tester Benazir remained exceptional by showing higher and significant specific combining ability (GCA) effects for majority traits including grain yield in F1 and F2 populations, as a result, both parents would contribute significantly to the improvement of the bread wheat. Regarding the SCA effects in F1 population, the hybrids TD-1 × Pakistan-2013, TJ-83 × Benazir, and NIA-Sundar × NIA-Sarang and from F2 populations, the crosses TD-1 × Benazir, TJ-83 × Benazir, Kiran-95 × NIA-Sarang and NIA-Amber × Pakistan-2013 expressed desirable and maximum SCA effects for number of traits including grain yield, thus may be preferred in future wheat breeding programs. Disease reaction on selected 18 F2 populations was performed, the introgression stripe rust resistance showed single dominant gene. The genetic analysis reported the involvement of major genes for stripe rust resistance. These findings could be used to grow high-yielding wheat lines that could have a profitable yield in stripe rust-prone areas.
采用随机完全区组设计(RCBD),三次重复,种植了 18 个 F1 和 F2 试验杂交种。在各品系中,TD-1 在 F1 和 F2 群体中对包括谷物产量在内的最大数量性状表现出更大且显著的一般结合能力(GCA)效应,居于首位;而试验者 Benazir 在 F1 和 F2 群体中对包括谷物产量在内的大多数性状表现出更大且显著的特异结合能力(GCA)效应,仍然出类拔萃。在F1群体的SCA效应方面,杂交种TD-1 × Pakistan-2013、TJ-83 × Benazir和NIA-Sundar × NIA-Sarang以及F2群体中的杂交种TD-1 × Benazir、TJ-83 × Benazir、Kiran-95 × NIA-Sarang和NIA-Amber × Pakistan-2013在包括谷粒产量在内的许多性状上表现出理想的最大SCA效应,因此在未来的小麦育种计划中可能会受到青睐。对所选的 18 个 F2 群体进行了病害反应研究,结果表明抗条锈病的引种表现为单显性基因。遗传分析表明,抗条锈病的主要基因参与了该基因的表达。这些发现可用于培育高产小麦品系,从而在条锈病多发地区提高产量。
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引用次数: 0
The Impact of Glucose-induced Priming on Nutrients Accumulation and Certain Primary Attributes of Brassica napus L. Under the Saline Regimes 盐碱地条件下葡萄糖诱导对甘蓝型油菜营养物质积累和某些主要特性的影响
Pub Date : 2024-02-14 DOI: 10.38211/joarps.2024.05.200
Ishtiaq Ahmad, Mohammad Hizar Kaleem, Rasheed Ahmad, Zeeshan Ahmad, Zahir Muhammad
In the present study, the response of primary attributes (seedling growth, percent emergence, seedling fresh and dry biomass, and seedling moisture contents) and nutrient contents (Calcium, Magneisum, Iron, Manganese, Zinc, Copper) of Brassica napus L. was evaluated as a function of glucose-induced priming and salinity. The priming comprised 30 minutes, 60 minutes and 90 minutes of seeds soaking in glucose solution (0.50 M) and salinity stress was simulated by the solutions of 0, 15, 18, 21, and 24 milli Molar (mM) NaCl. The results revealed that doses of salinity induced significant changes in the fresh weight of Brassica napus L. The response of selected nutrients (except Magnesium) as a function of salinity was also highly significant (P≤0.05 %). The salinity doses reduced plants' mineral contents (except Mn) compared to control. The priming of seeds for 90 minutes significantly (P≤0.05 %) enhanced certain early growth traits (plumule growth, radical growth, fresh weight and dry weight) of Brassica napus. On the other hand, for improving germination (%) and moisture contents (%) of Brassica napus, soaking durations of 30 minutes and 60 minutes are more suitable. The pre-soaking of seeds for 60 minutes increased the Calcium, Magnesium and Manganese contents (mg/litre) of Brassica napus. The Iron and Zinc contents (mg/litre) showed hype in seedlings raised from seeds primed for 30 minutes. The priming of seeds for 90 minutes was found to be stimulatory for Copper (mg/litre) only. The influence of factors interaction (treatments × priming durations) on the initial growth attributes and the studied minerals of Brassica napus L. was highly significant (P≤0.05 %). From the gathered evidence, the present study concludes glucose as a potent priming agent that can boost oil-yielding plants' performance under saline conditions.
本研究评估了甘蓝型油菜(Brassica napus L.)的主要属性(幼苗生长、出苗率、幼苗干鲜生物量和幼苗含水量)和养分含量(钙、镁、铁、锰、锌、铜)对葡萄糖诱导引诱和盐度的响应。诱导包括种子在葡萄糖溶液(0.50 M)中浸泡 30 分钟、60 分钟和 90 分钟,盐胁迫则通过 0、15、18、21 和 24 毫摩尔(mM)的氯化钠溶液来模拟。结果表明,不同剂量的盐分会导致甘蓝鲜重发生显著变化,某些营养元素(镁除外)对盐分的响应也非常显著(P≤0.05 %)。与对照相比,盐度降低了植物的矿物质含量(锰除外)。对种子进行 90 分钟的预处理可显著提高甘蓝型油菜的某些早期生长性状(羽叶生长、胚根生长、鲜重和干重)(P≤0.05 %)。另一方面,要提高甘蓝型油菜的发芽率(%)和含水量(%),浸种时间为 30 分钟和 60 分钟更为合适。浸种 60 分钟可提高油菜籽的钙、镁和锰含量(毫克/升)。用浸种 30 分钟的种子培育的幼苗中铁和锌的含量(毫克/升)有所提高。种子催芽 90 分钟仅对铜含量(毫克/升)有刺激作用。各因素交互作用(处理 × 引种时间)对甘蓝型油菜的初始生长属性和所研究的矿物质的影响非常显著(P≤0.05 %)。从收集到的证据来看,本研究认为葡萄糖是一种有效的引诱剂,可以提高植物在盐碱条件下的产油性能。
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引用次数: 0
The Impact of Glucose-induced Priming on Nutrients Accumulation and Certain Primary Attributes of Brassica napus L. Under the Saline Regimes 盐碱地条件下葡萄糖诱导对甘蓝型油菜营养物质积累和某些主要特性的影响
Pub Date : 2024-02-14 DOI: 10.38211/joarps.2024.05.200
Ishtiaq Ahmad, Mohammad Hizar Kaleem, Rasheed Ahmad, Zeeshan Ahmad, Zahir Muhammad
In the present study, the response of primary attributes (seedling growth, percent emergence, seedling fresh and dry biomass, and seedling moisture contents) and nutrient contents (Calcium, Magneisum, Iron, Manganese, Zinc, Copper) of Brassica napus L. was evaluated as a function of glucose-induced priming and salinity. The priming comprised 30 minutes, 60 minutes and 90 minutes of seeds soaking in glucose solution (0.50 M) and salinity stress was simulated by the solutions of 0, 15, 18, 21, and 24 milli Molar (mM) NaCl. The results revealed that doses of salinity induced significant changes in the fresh weight of Brassica napus L. The response of selected nutrients (except Magnesium) as a function of salinity was also highly significant (P≤0.05 %). The salinity doses reduced plants' mineral contents (except Mn) compared to control. The priming of seeds for 90 minutes significantly (P≤0.05 %) enhanced certain early growth traits (plumule growth, radical growth, fresh weight and dry weight) of Brassica napus. On the other hand, for improving germination (%) and moisture contents (%) of Brassica napus, soaking durations of 30 minutes and 60 minutes are more suitable. The pre-soaking of seeds for 60 minutes increased the Calcium, Magnesium and Manganese contents (mg/litre) of Brassica napus. The Iron and Zinc contents (mg/litre) showed hype in seedlings raised from seeds primed for 30 minutes. The priming of seeds for 90 minutes was found to be stimulatory for Copper (mg/litre) only. The influence of factors interaction (treatments × priming durations) on the initial growth attributes and the studied minerals of Brassica napus L. was highly significant (P≤0.05 %). From the gathered evidence, the present study concludes glucose as a potent priming agent that can boost oil-yielding plants' performance under saline conditions.
本研究评估了甘蓝型油菜(Brassica napus L.)的主要属性(幼苗生长、出苗率、幼苗干鲜生物量和幼苗含水量)和养分含量(钙、镁、铁、锰、锌、铜)对葡萄糖诱导引诱和盐度的响应。诱导包括种子在葡萄糖溶液(0.50 M)中浸泡 30 分钟、60 分钟和 90 分钟,盐胁迫则通过 0、15、18、21 和 24 毫摩尔(mM)的氯化钠溶液来模拟。结果表明,不同剂量的盐分会导致甘蓝鲜重发生显著变化,某些营养元素(镁除外)对盐分的响应也非常显著(P≤0.05 %)。与对照相比,盐度降低了植物的矿物质含量(锰除外)。对种子进行 90 分钟的预处理可显著提高甘蓝型油菜的某些早期生长性状(羽叶生长、胚根生长、鲜重和干重)(P≤0.05 %)。另一方面,要提高甘蓝型油菜的发芽率(%)和含水量(%),浸种时间为 30 分钟和 60 分钟更为合适。浸种 60 分钟可提高油菜籽的钙、镁和锰含量(毫克/升)。用浸种 30 分钟的种子培育的幼苗中铁和锌的含量(毫克/升)有所提高。种子催芽 90 分钟仅对铜含量(毫克/升)有刺激作用。各因素交互作用(处理 × 引种时间)对甘蓝型油菜的初始生长属性和所研究的矿物质的影响非常显著(P≤0.05 %)。从收集到的证据来看,本研究认为葡萄糖是一种有效的引诱剂,可以提高植物在盐碱条件下的产油性能。
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引用次数: 0
Estimation of Hetrosis in F1 Hybrids of Bread Wheat Genotypes 估算面包小麦基因型 F1 代杂交种的黑穗病发病率
Pub Date : 2024-01-09 DOI: 10.38211/joarps.2024.05.169
Ghulam Fareed, Dr. Ayaz Ali Keerio, S. Mari, M. A. Arain, Sana Ullah, A. A. Mastoi, Muhammad Adeel, Muhammad Aslam Mengal, Sajid Ali Shah, Muhammad Ilyas Badini
The experiment was conducted for estimation of the heterosis in F1 hybrids in wheat genotypes. The research was conducted at the experimental field of Seed Production Development Centre, Sindh Agriculture University Tandojam during Rabi growing season 2019-20. The experiment was laid-out in randomized complete block design (RCBD) using three replications. The performance of parents and their hybrids, and heterotic effects were measured in various aspects like earliness characters (days taken to 75% heading, days taken to 75% maturity), morphological and yield traits like plant height, tillers plant-1, yield plant-1, and a physiology related trait; relative water content. The study consisted of six varieties and their six F1 hybrids. The parents used were TD-1, Benazir-2013, TJ-83, Hamal, Imdad-2005, Kiran-95 and their crosses were as TD-1 × Hamal, TD-1 × Benazir-2013, Benazir-2013 × TD-1, TJ-83 × Benazir-2013, TJ-83 × Imdad, Kiran-95 ×Benazir-2013. Results revealed that all the parents and hybrids were highly significant for all the parameters studied at P<0.01 level. The F1 hybrids Benazir-2013 × TD-1, TD-1 × Benazir-2013, TD-1 × Hamal and Kiran -95 × Benazir-2013 had showed better response in mid parent heterosis and heterobeltiosis for most of the traits except plant height. On the basis of current findings, it could be concluded that these cross combinations may be used in bread wheat for yield improvement
该实验旨在估算小麦基因型 F1 杂交种的异质性。研究在信德农业大学种子生产发展中心的试验田进行,时间为 2019-20 年 Rabi 生长季节。实验采用随机完全区组设计(RCBD),三次重复。对亲本及其杂交种的表现和杂交效应进行了多方面的测定,如早熟性状(75% 发棵天数、75% 成熟天数)、形态和产量性状(株高、分蘖株数-1、产量株数-1)以及生理相关性状;相对含水量。研究包括六个品种及其六个 F1 代杂交种。亲本为 TD-1、Benazir-2013、TJ-83、Hamal、Imdad-2005、Kiran-95,杂交种为 TD-1×Hamal、TD-1×Benazir-2013、Benazir-2013×TD-1、TJ-83×Benazir-2013、TJ-83×Imdad、Kiran-95×Benazir-2013。结果表明,在 P<0.01 的水平上,所有亲本和杂交种对所有研究参数都具有高度显著性。F1 代杂交种 Benazir-2013×TD-1、TD-1×Benazir-2013、TD-1×Hamal 和 Kiran-95×Benazir-2013 在除株高外的大多数性状上表现出较好的亲本异交和异本同交反应。根据目前的研究结果,可以认为这些杂交组合可用于面包小麦的增产。
{"title":"Estimation of Hetrosis in F1 Hybrids of Bread Wheat Genotypes","authors":"Ghulam Fareed, Dr. Ayaz Ali Keerio, S. Mari, M. A. Arain, Sana Ullah, A. A. Mastoi, Muhammad Adeel, Muhammad Aslam Mengal, Sajid Ali Shah, Muhammad Ilyas Badini","doi":"10.38211/joarps.2024.05.169","DOIUrl":"https://doi.org/10.38211/joarps.2024.05.169","url":null,"abstract":"The experiment was conducted for estimation of the heterosis in F1 hybrids in wheat genotypes. The research was conducted at the experimental field of Seed Production Development Centre, Sindh Agriculture University Tandojam during Rabi growing season 2019-20. The experiment was laid-out in randomized complete block design (RCBD) using three replications. The performance of parents and their hybrids, and heterotic effects were measured in various aspects like earliness characters (days taken to 75% heading, days taken to 75% maturity), morphological and yield traits like plant height, tillers plant-1, yield plant-1, and a physiology related trait; relative water content. The study consisted of six varieties and their six F1 hybrids. The parents used were TD-1, Benazir-2013, TJ-83, Hamal, Imdad-2005, Kiran-95 and their crosses were as TD-1 × Hamal, TD-1 × Benazir-2013, Benazir-2013 × TD-1, TJ-83 × Benazir-2013, TJ-83 × Imdad, Kiran-95 ×Benazir-2013. Results revealed that all the parents and hybrids were highly significant for all the parameters studied at P<0.01 level. The F1 hybrids Benazir-2013 × TD-1, TD-1 × Benazir-2013, TD-1 × Hamal and Kiran -95 × Benazir-2013 had showed better response in mid parent heterosis and heterobeltiosis for most of the traits except plant height. On the basis of current findings, it could be concluded that these cross combinations may be used in bread wheat for yield improvement","PeriodicalId":197597,"journal":{"name":"Journal of Applied Research in Plant Sciences","volume":"19 S1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139628623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Zn Applied with or Without Palm Stearin Coated Urea on The Growth and Mineral Element Concentration of Maize (Zea mays L.) 施用或不施用棕榈硬脂包裹尿素的锌对玉米(Zea mays L.)生长和矿物质元素浓度的影响
Pub Date : 2024-01-08 DOI: 10.38211/joarps.2024.05.252
S. K. Babar, H. Akca, Süleyman Taban, Hassan Ali Shujrah
Inhibition of ammonia (NH3) volatilization by deaccelerating urea hydrolysis rate in Central Anatolian lands is the indispensable approach for eco-friendly fertilization and nitrogen use efficiency (NUE). Nitrogen (N) and zinc (Zn) are critically limited here in alkaline soils. An experiment was conducted under controlled conditions to determine the availability of Zn applied as a solution and bound with polymer palm stearin (PS) coating material as a urease inhibitor. The treatments consisted of urea as a commercial commodity, urea with PS only, urea impregnated with PS and Zn, Zn-coated urea, and Zn in solution (SOL) form. During winter, 2019-20, the experiment was conducted in the glasshouse of the department of Soil Science and Plant Nutrition at Ankara University, Türkiye. Data indicated that Zn with PS and in SOL form produced more growth traits i.e., plant height (130 cm), stem girth (13.2 mm), shoot dry matter (4.63 g plant-1), root dry matter yield (0.61 g plant-1), and chlorophyll (42.16 mg g-1) content (p<0.01). Similarly, we had higher concentration of N (3.19%) and Zn (50.46 mg kg-1) content in maize plants (p<0.01) as compared to control. In conclusion, Zn at the rate of 10 mg kg-1 either in solution or coated with urea seems highly effective to sustain better crop productivity and NUE. While concerning N and Zn content, coated urea with Zn markedly responded as compared to Zn in SOL. Synergism between N and Zn can lead to better fertilizer management
在安纳托利亚中部地区,通过降低尿素水解速度来抑制氨(NH3)的挥发,是生态友好型施肥和提高氮利用效率(NUE)不可或缺的方法。在碱性土壤中,氮(N)和锌(Zn)的用量非常有限。我们在受控条件下进行了一项实验,以确定以溶液形式施用并与作为脲酶抑制剂的聚合物棕榈硬脂(PS)涂层材料结合的锌的可用性。处理包括商品尿素、仅含 PS 的尿素、含 PS 和锌的尿素、锌包衣尿素以及溶液(SOL)形式的锌。实验于 2019-20 年冬季在土耳其安卡拉大学土壤科学与植物营养系的玻璃温室中进行。数据表明,PS和SOL形式的锌能产生更多的生长性状,即株高(130厘米)、茎围(13.2毫米)、芽干物质(4.63克/株-1)、根干物质产量(0.61克/株-1)和叶绿素(42.16毫克/克-1)含量(p<0.01)。同样,与对照组相比,玉米植株的氮浓度(3.19%)和锌含量(50.46 毫克/千克-1)也较高(p<0.01)。总之,10 毫克/千克的锌溶液或尿素包衣似乎对提高作物产量和氮利用效率非常有效。在氮和锌含量方面,与 SOL 中的锌相比,包膜尿素中的锌有明显的效果。氮和锌之间的协同作用可改善肥料管理
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引用次数: 0
Genome-wide Analysis of Plant Specific YABBY Transcription Factor Gene Family in Watermelon (Citrullus lanatus) and Arabidopsis 西瓜(Citrullus lanatus)和拟南芥中植物特异性 YABBY 转录因子基因家族的全基因组分析
Pub Date : 2023-11-13 DOI: 10.38211/joarps.2024.05.179
Mehr-ul- Nisa, Muhammad Shafiq, M. Manzoor, Muhammad Bilal, Tariq Manzoor, Malik Muazzam Anees, Muhammad Rizwan, Muhammad Zeeshan Haider, Adnan Sami, Muhammad Saleem Haider
The YABBY gene family is a specific transcription factor for plants and a DNA binding domain that carries out several different functions, such as regulating the length of blooming plant styles and the polarity of lateral organ development. The YABBY gene family members were identified in the watermelon (Citrullus lanatus subsp. vulgaris var. 97103 V1) genome using a set of bioinformatics techniques. Protein motifs, protein architectures, protein sequences, miRNA targets, and tissue-specific expression patterns were all examined. All chromosomes had an uneven distribution of about eight putative YABBY genes. Inner No Outer INO, CRC (Crabs Claw), YAB2, YAB3/AFO, and YAB5 were the five subgroups that the YABBY proteins in watermelon fall within, in accordance with the accepted Arabidopsis categorization which is based on International Standards of Nomenclature. Segmental duplication was more frequent than tandem duplication, and it was predominantly responsible for the growth of the YABBY gene family in watermelon. The results of tissue-specific expression profiling of ClYAABY genes showed that the vast majority of these genes were substantially expressed in roots and seedlings. In this study, cis-regulatory element (CRE) analyses were employed to identify elements in seedlings and roots that are highly responsive to light,wound, drought, auxin, stress, salicylic acid, and abscisic acid (ABA). The findings reveal specific CREs within the promoter regions of genes associated with these responses. Five groups or sub-families have also been identified by comparing the YABBY genes in watermelon and Arabidopsis, however the CRC and YAB2 groups do not share gene pairing among the other groups. This research contributes to a deeper understanding of plant adaptability and stress response mechanisms, with implications for agriculture and plant science.
YABBY 基因家族是植物的一种特异性转录因子,其 DNA 结合域具有多种不同的功能,如调节开花植物花柱的长度和侧生器官发育的极性。利用一套生物信息学技术,在西瓜(Citrullus lanatus subsp.研究人员对蛋白质基序、蛋白质结构、蛋白质序列、miRNA靶标和组织特异性表达模式进行了研究。所有染色体上的约八个假定 YABBY 基因分布不均。根据基于国际命名标准的拟南芥公认分类法,西瓜中的 YABBY 蛋白分为内无外 INO、CRC(蟹爪)、YAB2、YAB3/AFO 和 YAB5 五个亚组。片段重复比串联重复更为常见,它是西瓜中 YABBY 基因家族增长的主要原因。ClYAABY 基因的组织特异性表达谱分析结果表明,这些基因绝大多数在根和幼苗中大量表达。本研究利用顺式调控元件(CRE)分析,确定了幼苗和根系中对光照、伤口、干旱、辅助素、胁迫、水杨酸和脱落酸(ABA)高度敏感的元件。研究结果揭示了与这些反应相关的基因启动子区域中的特定 CRE。通过比较西瓜和拟南芥中的 YABBY 基因,还发现了五个组或亚族,但 CRC 和 YAB2 组与其他组的基因配对并不相同。这项研究有助于加深对植物适应性和胁迫响应机制的理解,对农业和植物科学具有重要意义。
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引用次数: 0
DNA Barcoding Based Identification of Rosa x Damascene and Prunus dulcis Herbs Using ITS2 Barcoding Gene Amplification. 利用 ITS2 条形码基因扩增技术,基于 DNA 条形码鉴定大马士革蔷薇和杜鹃花。
Pub Date : 2023-11-13 DOI: 10.38211/joarps.2024.05.219
Syed Rizwan, Hakim Abdul Bari, Arshad Saleem, Jingyuan Song, Tianyi Xin, Muhammad Jahanzeb
The considerable risk of adulteration in the herbs has raised commercial interest in the identification of medicinal herbs globally. DNA barcoding is the primary techniques for identifying the herbs at genetic level. This technique's key benefit is that it can identify the material's purity. This study focuses on the accurate identification of species utilizing Polymerase chain reaction-based nuclear universal internal transcribed spacer region (a barcode region) amplification and sequencing in 2 medically significant plants (Rosa damascene and Prunus dulcis) procured from the local herbal market of Karachi in the year 2022. Results suggest that the ribosomal nuclear ITS2 region of the selected plant species shows the 100 % identity with the reference genome, therefore it has shown a good rate of identification at the species level. The findings of this concludes that ITS2 is the novel standard barcode that involve in species identification, genomic conservation, and secure utilization of the medically significant plant species.
药材掺假的风险相当大,这引起了全球对药材鉴定的商业兴趣。DNA 条形码是在基因水平上鉴定药材的主要技术。这项技术的主要优点是可以鉴定材料的纯度。本研究的重点是利用基于聚合酶链式反应的核通用内部转录间隔区(条形码区)扩增和测序技术,对 2022 年从卡拉奇当地草药市场采购的 2 种具有重要药用价值的植物(大马士革蔷薇和杜梨)进行准确的物种鉴定。结果表明,所选植物物种的核糖体核 ITS2 区域与参考基因组显示出 100 % 的一致性,因此在物种水平上显示出良好的鉴定率。研究结果表明,ITS2 是一种新型的标准条形码,可用于物种鉴定、基因组保护以及对具有重要药用价值的植物物种的安全利用。
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引用次数: 0
Identification and Quantification of Phenolic Compounds by HPLC-DAD and Antioxidant activity from Cordia Gharaf Plant HPLC-DAD法鉴定、定量测定枸杞中酚类化合物及其抗氧化活性
Pub Date : 2023-05-26 DOI: 10.38211/joarps.2023.04.02.159
Munwar Hussain Laghari, J. Mangi, Mudasir Gorar, S. Sohu, Asma Hayat, S. Zahid, Abdul Waheed Chaudhary, Farooque Azam Khatri, Khadim Ali Gishkori, Muhammad Yousif Seelro, Arslan Qureshi, Sikandar Ali Soomro, A. R. Sidhu
Numerous indigenous plants may grow on Pakistan's soil due to its suitable climate. A few of these are now exploited as a source of expensive pharmaceuticals with therapeutic benefits. Since 4000-5000 B.C., people have used these native herbs as medicines. The Cordia gharaf plant is one of them. There are several phytochemicals found in plants, including flavonoids, alkaloids, tannins, phenolic compounds, and others, that can protect against disease. Many phytochemicals have a broad range of biological activities that help to protect against chronic illnesses, and around the world, they have been used to treat several different human ailments. According to a World Health Organization (WHO) report, more than 80% of the world's population uses traditional medicines for treatment and maintaining their health, and many valuable medications have been extracted from these medicinal plants. Therefore, scientific investigation is necessary to make enormous profits from these priceless medicinal plants. Due to the importance of the primary and secondary metabolites produced by these medicinal plants, our purpose includes the idea of examining biologically active components from these plants. Keeping in mind the foregoing, the goal of our research is to examine the phytochemicals from the selected species of Cordia gharaf that have disease-preventive properties.
由于气候适宜,许多本土植物可以在巴基斯坦的土壤上生长。其中一些现在被开发为具有治疗作用的昂贵药物的来源。自公元前4000-5000年以来,人们就开始使用这些本土草药作为药物。Cordia garaf植物就是其中之一。在植物中发现了几种植物化学物质,包括类黄酮、生物碱、单宁、酚类化合物等,它们可以预防疾病。许多植物化学物质具有广泛的生物活性,有助于预防慢性疾病,在世界各地,它们已被用于治疗几种不同的人类疾病。根据世界卫生组织(WHO)的一份报告,世界上80%以上的人口使用传统药物来治疗和维持健康,许多有价值的药物都是从这些药用植物中提取的。因此,为了从这些无价的药用植物中获取巨大的利润,进行科学调查是必要的。由于这些药用植物产生的初级和次级代谢物的重要性,我们的目的包括检查这些植物的生物活性成分的想法。记住上述内容,我们研究的目标是检查从选定的Cordia gharaf物种中具有预防疾病特性的植物化学物质。
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引用次数: 0
Assessment of Selected Trace Metals in Commonly Consumed Canned and Raw Food Products in Sindh, Pakistan 对巴基斯坦信德省常食用的罐头和生食品中选定微量金属的评估
Pub Date : 2023-05-26 DOI: 10.38211/joarps.2023.04.02.152
Amjad Ali Maitlo, Sanaullah Ansari, Amjad Hussain Soomro, A. Memon, Insaf Raza Mughal, G. Jaffar
The current study uses a flame atomic absorption spectrometer to quantify seven trace elements—Chromium (Cr), Cobalt (Co), Zinc (Zn), Manganese (Mn), Sodium (Na), Potassium (K), and Calcium (Ca) in canned and raw food products sold in Sukkur and Khairpur, Sindh, Pakistan. Current study results were compared to WHO/FAO maximum and minimum limits. Most samples were contaminated with trace elements (likely from anthropogenic activities, local industries, and brick kilns) and exceeded the allowable limits. Food products had varied metal content. In canned food products, Cr was max in peas (6.99 mg/kg) and min in pineapple (3.08 mg/kg), Co was max in pineapple (3.11 mg/kg) and min in honey (1.50 mg/kg), Zn was max in tamarind (5.77 mg/kg) and min in pineapple and mushroom (2.95 mg/kg), Mn was max in ginger (0.39 mg/kg) and min in pineapple and mangoes (0.24 mg/kg), Na was max in ginger (167 mg/kg) and min in honey (76.7 mg/kg), and K was max in tamarind (4942 mg/kg) and min in honey (76.7 mg In raw food products, Cr was highest in carrot (6.21 mg/kg) and lowest in pineapple (3.08 mg/kg), Co was highest in pineapple (3.24 mg/kg) and lowest in carrot (1.14 mg/kg), Zn was highest in carrot (6.33 mg/kg) and lowest in pineapple and honey (2.96 mg/kg), Mn was highest in ginger (0.41 mg/kg) and lowest in honey (0.24 mg/kg), Na was highest in garlic (202 mg/kg) and lowest in strawberries (65.1 mg/kg), and K was highest in tamarind (4963 mg/kg) and lowest in butter. In conclusion, to ensure public safety, these elements must be monitored in canned and raw food products
目前的研究使用火焰原子吸收光谱仪量化了在巴基斯坦信德省苏库尔和凯布尔销售的罐头和生食品中的七种微量元素——铬(Cr)、钴(Co)、锌(Zn)、锰(Mn)、钠(Na)、钾(K)和钙(Ca)。目前的研究结果与世卫组织/粮农组织的最高和最低限度进行了比较。大多数样品被微量元素污染(可能来自人为活动、当地工业和砖窑),超过了允许的限度。食品的金属含量各不相同。在罐头食品,Cr马克斯在豌豆(6.99毫克/公斤)和最小菠萝(3.08毫克/公斤),公司在菠萝马克斯(3.11毫克/公斤)和最小蜂蜜(1.50毫克/公斤),锌在罗望子马克斯(5.77毫克/公斤)和最小菠萝和蘑菇(2.95毫克/公斤),Mn马克斯在姜(0.39毫克/公斤)和最小菠萝和芒果(0.24毫克/公斤),Na马克斯在姜(167毫克/公斤)和最小的蜂蜜(76.7毫克/公斤),和K在罗望子马克斯(4942毫克/公斤)蜂蜜和最小(76.7毫克的生食产品,Cr含量在胡萝卜中最高(6.21 mg/kg),在菠萝中最低(3.08 mg/kg); Co含量在菠萝中最高(3.24 mg/kg),在胡萝卜中最低(1.14 mg/kg); Zn含量在胡萝卜中最高(6.33 mg/kg),在菠萝和蜂蜜中最低(2.96 mg/kg); Mn含量在生姜中最高(0.41 mg/kg),在蜂蜜中最低(0.24 mg/kg); Na含量在大蒜中最高(202 mg/kg),在草莓中最低(65.1 mg/kg);总之,为了确保公共安全,必须对罐头和生食品中的这些元素进行监测
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Journal of Applied Research in Plant Sciences
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