Pub Date : 2023-08-07DOI: 10.1590/s1678-3921.pab2023.v58.03153
Daniela Rocha da Silva, O. A. D. Costa, A. C. Fluck, B. Menezes, A. Baungratz, V. P. Macedo
Abstract The objective of this work was to compare the zootechnical performance of Dorper x Santa Inês crossbred lambs finished in grazing systems on Megathyrsus maximus Aruana cultivar pasture, with and without shading, and in a feedlot. Twenty-four non-castrated lambs were used in a completely randomized experimental design, with the three following treatments with eight replicates each: 'Aruana' pasture without shading + supplement; 'Aruana' pasture with shading + supplement; and feedlot, with diet containing ryegrass hay and concentrate at a 20:80 ratio. The treatments did not influence lamb age at slaughter, birth weight, leg compactness, true yield, and main cut yield, except that of breast tip + flank that was lower for the pasture with shading. However, average daily gain, body condition score, live weight at slaughter, carcass compactness index, cold carcass and empty body weights, and cold carcass yield differed between treatments, showing higher values in the feedlot. Termination in the feedlot increases the zootechnical performance of Dorper x Santa Inês sheep and favors a lower age at slaughter, as well as a greater weight of the main commercial cuts, but not their yield.
{"title":"Zootechnical performance of Dorper x Santa Inês lambs raised in different production systems","authors":"Daniela Rocha da Silva, O. A. D. Costa, A. C. Fluck, B. Menezes, A. Baungratz, V. P. Macedo","doi":"10.1590/s1678-3921.pab2023.v58.03153","DOIUrl":"https://doi.org/10.1590/s1678-3921.pab2023.v58.03153","url":null,"abstract":"Abstract The objective of this work was to compare the zootechnical performance of Dorper x Santa Inês crossbred lambs finished in grazing systems on Megathyrsus maximus Aruana cultivar pasture, with and without shading, and in a feedlot. Twenty-four non-castrated lambs were used in a completely randomized experimental design, with the three following treatments with eight replicates each: 'Aruana' pasture without shading + supplement; 'Aruana' pasture with shading + supplement; and feedlot, with diet containing ryegrass hay and concentrate at a 20:80 ratio. The treatments did not influence lamb age at slaughter, birth weight, leg compactness, true yield, and main cut yield, except that of breast tip + flank that was lower for the pasture with shading. However, average daily gain, body condition score, live weight at slaughter, carcass compactness index, cold carcass and empty body weights, and cold carcass yield differed between treatments, showing higher values in the feedlot. Termination in the feedlot increases the zootechnical performance of Dorper x Santa Inês sheep and favors a lower age at slaughter, as well as a greater weight of the main commercial cuts, but not their yield.","PeriodicalId":19826,"journal":{"name":"Pesquisa Agropecuaria Brasileira","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67610628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-07DOI: 10.1590/s1678-3921.pab2023.v58.03026
Gustavo Sosa-Pérez, S. López-Ortiz, P. Pérez-Hernández, H. Vaquera-Huerta, M. C. Galván, J. Gallegos-Sánchez
Abstract The objective of this work was to determine the nutritional content and the degradability of the dry matter and protein of fruits of Chloroleucon mangense and Acacia cochliacantha and of a 1:1 mixture of both, offered as supplements to Rambouillet sheep. In situ ruminal degradation was evaluated in three adult rams, fitted with a rumen cannula, with different incubation times of 0, 6, 12, 24, 48, and 72 hours. Protein intestinal degradability was quantified with a three-step procedure: in situ ruminal incubation, in vitro enzymatic digestion, and abomasal-intestinal digestion. The fruits of C. mangense and A. cochliacantha contain 21 and 12% crude protein, 47 and 56% neutral detergent fiber, 31 and 43% acid detergent fiber, and 0.9 and 6.0% condensed tannins, respectively. The fruits of C. mangense showed a higher nutritional value and a higher dry matter and crude protein degradability (p<0.05) than those of A. cochliacantha and the 1:1 mixture. The amount of protein that reaches the small intestine is higher for the 1:1, which is an indicative that its tannin concentration is enough to increase the bypass protein that can be absorbed in the small intestine.
{"title":"Degradability of the dry matter and crude protein of fruits of Chloroleucon mangense and Acacia cochliacantha in sheep","authors":"Gustavo Sosa-Pérez, S. López-Ortiz, P. Pérez-Hernández, H. Vaquera-Huerta, M. C. Galván, J. Gallegos-Sánchez","doi":"10.1590/s1678-3921.pab2023.v58.03026","DOIUrl":"https://doi.org/10.1590/s1678-3921.pab2023.v58.03026","url":null,"abstract":"Abstract The objective of this work was to determine the nutritional content and the degradability of the dry matter and protein of fruits of Chloroleucon mangense and Acacia cochliacantha and of a 1:1 mixture of both, offered as supplements to Rambouillet sheep. In situ ruminal degradation was evaluated in three adult rams, fitted with a rumen cannula, with different incubation times of 0, 6, 12, 24, 48, and 72 hours. Protein intestinal degradability was quantified with a three-step procedure: in situ ruminal incubation, in vitro enzymatic digestion, and abomasal-intestinal digestion. The fruits of C. mangense and A. cochliacantha contain 21 and 12% crude protein, 47 and 56% neutral detergent fiber, 31 and 43% acid detergent fiber, and 0.9 and 6.0% condensed tannins, respectively. The fruits of C. mangense showed a higher nutritional value and a higher dry matter and crude protein degradability (p<0.05) than those of A. cochliacantha and the 1:1 mixture. The amount of protein that reaches the small intestine is higher for the 1:1, which is an indicative that its tannin concentration is enough to increase the bypass protein that can be absorbed in the small intestine.","PeriodicalId":19826,"journal":{"name":"Pesquisa Agropecuaria Brasileira","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67609691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-26DOI: 10.1590/s1678-3921.pab2023.v58.03084
Júlio Daronco Berlezi, I. Carvalho, J. P. D. Silva, Murilo Vieira Loro, Inaê Carolina Sfalcin, Leonardo Cesar Pradebon, E. D. Port, Rafael Soares Ourique, João Pedro Dalla Roza
Abstract The objective of this work was to select white oat (Avena sativa) genotypes for environments with and without fungicide application, as well as to identify relationships between meteorological variables and agronomic traits. Two experiments were carried out: one without and the other with the use of the tebuconazole fungicide. In each experiment, 26 genotypes were evaluated, conducted during ten years in the same experimental area; cultivation years were considered as one environment. The methodologies of genotype and of the genotypes x environments interaction were used to define the ideal genotype. 'URS Corona' is considered an ideal genotype due to its excellent responses in the environment with tebuconazole. The 'Brisasul', 'IPR Aphrodite', and 'URS Taura' genotypes respond better in the environment without the fungicide. However, based on multitraits, the 'URS Taura' and 'IPR Afrodite' genotypes can be positioned in environments with or without the use of tebuconazole. Genotypes with a rust incidence lower than 15% and a height higher than 93 cm in an environment with a relative humidity lower than 70.75% have a higher grain yield regardless of fungicide use. Greater magnitudes of air temperature and solar radiation result in a higher grain yield of white oat, regardless of fungicide use.
{"title":"Selection of white oat genotypes for contrasting fungicide management conditions","authors":"Júlio Daronco Berlezi, I. Carvalho, J. P. D. Silva, Murilo Vieira Loro, Inaê Carolina Sfalcin, Leonardo Cesar Pradebon, E. D. Port, Rafael Soares Ourique, João Pedro Dalla Roza","doi":"10.1590/s1678-3921.pab2023.v58.03084","DOIUrl":"https://doi.org/10.1590/s1678-3921.pab2023.v58.03084","url":null,"abstract":"Abstract The objective of this work was to select white oat (Avena sativa) genotypes for environments with and without fungicide application, as well as to identify relationships between meteorological variables and agronomic traits. Two experiments were carried out: one without and the other with the use of the tebuconazole fungicide. In each experiment, 26 genotypes were evaluated, conducted during ten years in the same experimental area; cultivation years were considered as one environment. The methodologies of genotype and of the genotypes x environments interaction were used to define the ideal genotype. 'URS Corona' is considered an ideal genotype due to its excellent responses in the environment with tebuconazole. The 'Brisasul', 'IPR Aphrodite', and 'URS Taura' genotypes respond better in the environment without the fungicide. However, based on multitraits, the 'URS Taura' and 'IPR Afrodite' genotypes can be positioned in environments with or without the use of tebuconazole. Genotypes with a rust incidence lower than 15% and a height higher than 93 cm in an environment with a relative humidity lower than 70.75% have a higher grain yield regardless of fungicide use. Greater magnitudes of air temperature and solar radiation result in a higher grain yield of white oat, regardless of fungicide use.","PeriodicalId":19826,"journal":{"name":"Pesquisa Agropecuaria Brasileira","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67609971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-26DOI: 10.1590/s1678-3921.pab2023.v58.03058
P. Goergen, S. J. Lopes, A. Zanon, I. Lago, Valéria Pohlmann, Menigui Spanevello Dalcin, Pâmela Nunes Bittencourt, Vanessa Gonçalves Saccol
Abstract The objective of this work was to evaluate the tolerance of soybean (Glycine max) cultivars to flooding stress at different growth stages. The experiment was arranged in a 2x2x5 factorial arrangement, with two sowing dates (October and November), two soybean cultivars (TECIRGA 6070RR and NA 5909 RG), and five growth stages (SE-EM, EM-VC, VC-V2, V2-V4, and V6-V8) in the 2018/2019 and 2019/2020 crop years. The experimental design was completely randomized with four replicates. For both evaluated soybean cultivars, the growth stage most sensitive to soil flooding was SE-EM. After seedling emergence, the highest reductions in leaf area and shoot dry matter were observed in the V2-V4 stage, regardless of cultivar, sowing date, and crop year. From the V4 stage onwards, soybean shows a higher tolerance to flooding conditions. However, after seedling emergence, the TECIRGA 6070RR cultivar has a higher tolerance to flooding stress than NA 5909 RG. Sowing in October tends to reduce the impact of flooding stress on the plants.
{"title":"Tolerance of soybean cultivars to flooding stress in vegetative growth stages","authors":"P. Goergen, S. J. Lopes, A. Zanon, I. Lago, Valéria Pohlmann, Menigui Spanevello Dalcin, Pâmela Nunes Bittencourt, Vanessa Gonçalves Saccol","doi":"10.1590/s1678-3921.pab2023.v58.03058","DOIUrl":"https://doi.org/10.1590/s1678-3921.pab2023.v58.03058","url":null,"abstract":"Abstract The objective of this work was to evaluate the tolerance of soybean (Glycine max) cultivars to flooding stress at different growth stages. The experiment was arranged in a 2x2x5 factorial arrangement, with two sowing dates (October and November), two soybean cultivars (TECIRGA 6070RR and NA 5909 RG), and five growth stages (SE-EM, EM-VC, VC-V2, V2-V4, and V6-V8) in the 2018/2019 and 2019/2020 crop years. The experimental design was completely randomized with four replicates. For both evaluated soybean cultivars, the growth stage most sensitive to soil flooding was SE-EM. After seedling emergence, the highest reductions in leaf area and shoot dry matter were observed in the V2-V4 stage, regardless of cultivar, sowing date, and crop year. From the V4 stage onwards, soybean shows a higher tolerance to flooding conditions. However, after seedling emergence, the TECIRGA 6070RR cultivar has a higher tolerance to flooding stress than NA 5909 RG. Sowing in October tends to reduce the impact of flooding stress on the plants.","PeriodicalId":19826,"journal":{"name":"Pesquisa Agropecuaria Brasileira","volume":"42 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67609924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-26DOI: 10.1590/s1678-3921.pab2023.v58.03042
F. E. C. Costa, Samanda López Peña, W. A. Pereira
Abstract The objective of this work was to evaluate the effects of the application of the glyphosate herbicide on seed reserve mobilization and seedling morphology of genetically modified soybean. Two herbicide-tolerant (TMG 1264RR and P98Y11) and two herbicide-sensitive (Emgopa 315 and UFUS 7415) cultivars were selected for the study and subjected to germination, seedling length, and reserve mobilization tests after treatments with glyphosate solutions at the concentrations of 0.00, 0.06, and 0.12%. The hypocotyl/radicle ratio and the efficiency of conversion of reserves to seedlings were also determined. The higher the concentration of glyphosate, the lower the percentage of normal seedlings and the shorter seedling length, mainly in the herbicide-sensitive cultivars. The 'TMG 1264RR' glyphosate-tolerant genotype mobilized more reserves and was more efficient in converting biomass into seedlings. Herbicide application reduced the average length of the seedlings and caused the roots to become shorter than the hypocotyls. During germination, the herbicide changes seedling morphology since the seedling hypocotyl becomes proportionally larger than the radicle. Although, when applied, glyphosate altered the length, weight, and reserve mobilization of the four evaluated genotypes, the most affected were 'Emgopa 315' and 'UFUS 7415', the glyphosate-sensitive ones.
{"title":"Seed reserve mobilization and seedling morphology of genetically modified soybean treated with glyphosate","authors":"F. E. C. Costa, Samanda López Peña, W. A. Pereira","doi":"10.1590/s1678-3921.pab2023.v58.03042","DOIUrl":"https://doi.org/10.1590/s1678-3921.pab2023.v58.03042","url":null,"abstract":"Abstract The objective of this work was to evaluate the effects of the application of the glyphosate herbicide on seed reserve mobilization and seedling morphology of genetically modified soybean. Two herbicide-tolerant (TMG 1264RR and P98Y11) and two herbicide-sensitive (Emgopa 315 and UFUS 7415) cultivars were selected for the study and subjected to germination, seedling length, and reserve mobilization tests after treatments with glyphosate solutions at the concentrations of 0.00, 0.06, and 0.12%. The hypocotyl/radicle ratio and the efficiency of conversion of reserves to seedlings were also determined. The higher the concentration of glyphosate, the lower the percentage of normal seedlings and the shorter seedling length, mainly in the herbicide-sensitive cultivars. The 'TMG 1264RR' glyphosate-tolerant genotype mobilized more reserves and was more efficient in converting biomass into seedlings. Herbicide application reduced the average length of the seedlings and caused the roots to become shorter than the hypocotyls. During germination, the herbicide changes seedling morphology since the seedling hypocotyl becomes proportionally larger than the radicle. Although, when applied, glyphosate altered the length, weight, and reserve mobilization of the four evaluated genotypes, the most affected were 'Emgopa 315' and 'UFUS 7415', the glyphosate-sensitive ones.","PeriodicalId":19826,"journal":{"name":"Pesquisa Agropecuaria Brasileira","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67609769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-26DOI: 10.1590/s1678-3921.pab2023.v58.03102
D. Moreno, R. M. Ortega, M. F. Paulino, L. N. Rennó, E. Detmann
Abstract The objective of this work was to evaluate the effect of energy-protein supplementation in preand postpartum periods on the productive and metabolic responses of grazing beef cows on tropical pasture. A group of 48 multiparous Nellore cows, with an initial gestation period of 6.4 months, body weight (BW) of 514.9 kg, and body condition score (BCS) of 5.4, was distributed in a completely randomized design, in a 2×2 factorial arrangement. The evaluated treatments were: UNS-UNS, unsupplemented during prepartum and postpartum; UNS-SUP, unsupplemented during prepartum and supplemented during postpartum; SUP-UNS, supplemented during prepartum and unsupplemented during postpartum; and SUP-SUP, supplemented during preand postpartum. The energy-protein supplement was offered at an amount of 1.5 kg per animal per day. Prepartum supplementation increased average daily gain and BCS at calving. Prepartum supplementation reduced non-esterified fatty acids (NEFA) and increased serum concentrations of total proteins and globulins during postpartum. Postpartum supplementation increased the BCS of cows and BW of calves at the end of the experiment. Postpartum supplementation increased blood concentrations of progesterone, while NEFA decreased. Supplementation with 1.5 kg per day of the energy-protein supplement during the last 84 days of gestation improves productive performance and metabolic status during the preand postpartum periods of grazing cows.
{"title":"Pre-and postpartum supplementation strategies on the performance and metabolic status of grazing beef cows","authors":"D. Moreno, R. M. Ortega, M. F. Paulino, L. N. Rennó, E. Detmann","doi":"10.1590/s1678-3921.pab2023.v58.03102","DOIUrl":"https://doi.org/10.1590/s1678-3921.pab2023.v58.03102","url":null,"abstract":"Abstract The objective of this work was to evaluate the effect of energy-protein supplementation in preand postpartum periods on the productive and metabolic responses of grazing beef cows on tropical pasture. A group of 48 multiparous Nellore cows, with an initial gestation period of 6.4 months, body weight (BW) of 514.9 kg, and body condition score (BCS) of 5.4, was distributed in a completely randomized design, in a 2×2 factorial arrangement. The evaluated treatments were: UNS-UNS, unsupplemented during prepartum and postpartum; UNS-SUP, unsupplemented during prepartum and supplemented during postpartum; SUP-UNS, supplemented during prepartum and unsupplemented during postpartum; and SUP-SUP, supplemented during preand postpartum. The energy-protein supplement was offered at an amount of 1.5 kg per animal per day. Prepartum supplementation increased average daily gain and BCS at calving. Prepartum supplementation reduced non-esterified fatty acids (NEFA) and increased serum concentrations of total proteins and globulins during postpartum. Postpartum supplementation increased the BCS of cows and BW of calves at the end of the experiment. Postpartum supplementation increased blood concentrations of progesterone, while NEFA decreased. Supplementation with 1.5 kg per day of the energy-protein supplement during the last 84 days of gestation improves productive performance and metabolic status during the preand postpartum periods of grazing cows.","PeriodicalId":19826,"journal":{"name":"Pesquisa Agropecuaria Brasileira","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67610150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-26DOI: 10.1590/s1678-3921.pab2023.v58.02863
Ezequiel Luís Vidal, S. D. S. Camargo, F. Cardoso
Abstract The objective of this work was to propose a new stopping criterion to shorten the computing time of the PampaPlus genetic improvement software, while maximizing the genetic qualification index (GQI) of the progeny, controlling inbreeding, and avoiding unintended culling. Data from two beef-cattle herds integrating PampaPlus were used. Five mating scenarios were built using different numbers of sires (9 to 37) and dams (142 to 568). The analyzed algorithm inputs were: expected progeny differences, pedigree information, maximum inbreeding, maximum and minimum number of matches for each sire, and penalty weights for poor performance. The analyzed response variables were computing time and the GQI of the progenies. Three stopping criteria were used: original stopping criterion fixed at 1,000 iterations; saturation stopping criterion (SSC), based on GQI variance; and Bhandari’s stopping criterion (BSC), which includes the generation interval parameter. SSC and BSC reduced processing time in 24.43-53.64% and in 14.32-50.87%, respectively. BSC reaches solution in less time, without losses in GQI quality. BSC is generalizable and effective to reduce the processing time of mating recommendations.
{"title":"Stopping criteria for genetic improvement software for beef-cattle mating selection","authors":"Ezequiel Luís Vidal, S. D. S. Camargo, F. Cardoso","doi":"10.1590/s1678-3921.pab2023.v58.02863","DOIUrl":"https://doi.org/10.1590/s1678-3921.pab2023.v58.02863","url":null,"abstract":"Abstract The objective of this work was to propose a new stopping criterion to shorten the computing time of the PampaPlus genetic improvement software, while maximizing the genetic qualification index (GQI) of the progeny, controlling inbreeding, and avoiding unintended culling. Data from two beef-cattle herds integrating PampaPlus were used. Five mating scenarios were built using different numbers of sires (9 to 37) and dams (142 to 568). The analyzed algorithm inputs were: expected progeny differences, pedigree information, maximum inbreeding, maximum and minimum number of matches for each sire, and penalty weights for poor performance. The analyzed response variables were computing time and the GQI of the progenies. Three stopping criteria were used: original stopping criterion fixed at 1,000 iterations; saturation stopping criterion (SSC), based on GQI variance; and Bhandari’s stopping criterion (BSC), which includes the generation interval parameter. SSC and BSC reduced processing time in 24.43-53.64% and in 14.32-50.87%, respectively. BSC reaches solution in less time, without losses in GQI quality. BSC is generalizable and effective to reduce the processing time of mating recommendations.","PeriodicalId":19826,"journal":{"name":"Pesquisa Agropecuaria Brasileira","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67609957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-29DOI: 10.1590/s1678-3921.pab2023.v58.03000
A. Koltun, Nathalia Volpi e Silva, Jéssika Angelotti-Mendonça, S. R. Marin, L. Gonçalves, A. Nepomuceno, L. M. Mertz-Henning
Abstract The objective of this work was to develop a method to create and validate CRISPR-Cas systems and different gRNAs in soybean (Glycine max) embryos. Two model genes were used for simple mutation with one gRNA or partial gene deletion with two guides. The gRNAs were inserted into the CRISPR transformation vectors by a type IIS restriction enzyme or by subcloning and inserting the promoter + gRNA2 in the final transformation vector using the classic restriction enzyme cloning method. The vectors were successfully constructed for one and two gRNAs. Agrobacterium-mediated transient transformation in soybean was carried out to test the quality of gRNAs and of the system itself (expression cassette). Simple mutation and gene deletion were detected in the embryos transformed after DNA enrichment by enzyme digestion followed by polymerase chain reaction and sequencing, which indicates that the CRISPR-Cas system and guides were working. This protocol can be used to accelerate CRISPR-based genome editing strategies for genetic transformation in soybean.
{"title":"CRISPR-transient expression in soybean for simplified gRNA screening in planta","authors":"A. Koltun, Nathalia Volpi e Silva, Jéssika Angelotti-Mendonça, S. R. Marin, L. Gonçalves, A. Nepomuceno, L. M. Mertz-Henning","doi":"10.1590/s1678-3921.pab2023.v58.03000","DOIUrl":"https://doi.org/10.1590/s1678-3921.pab2023.v58.03000","url":null,"abstract":"Abstract The objective of this work was to develop a method to create and validate CRISPR-Cas systems and different gRNAs in soybean (Glycine max) embryos. Two model genes were used for simple mutation with one gRNA or partial gene deletion with two guides. The gRNAs were inserted into the CRISPR transformation vectors by a type IIS restriction enzyme or by subcloning and inserting the promoter + gRNA2 in the final transformation vector using the classic restriction enzyme cloning method. The vectors were successfully constructed for one and two gRNAs. Agrobacterium-mediated transient transformation in soybean was carried out to test the quality of gRNAs and of the system itself (expression cassette). Simple mutation and gene deletion were detected in the embryos transformed after DNA enrichment by enzyme digestion followed by polymerase chain reaction and sequencing, which indicates that the CRISPR-Cas system and guides were working. This protocol can be used to accelerate CRISPR-based genome editing strategies for genetic transformation in soybean.","PeriodicalId":19826,"journal":{"name":"Pesquisa Agropecuaria Brasileira","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67610047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-29DOI: 10.1590/s1678-3921.pab2023.v58.03099
A. S. O. Santos, H. P. Pereira, G. N. Fogaça, V. M. Meurer, M. Furtado, Cristiano Amâncio Vieira Borges, M. Weller, M. Martins
Abstract The objective of this work was to evaluate microfluidic chip electrophoresis, known as lab-on-a-chip technique, for the detection of milk adulteration using cheese whey in comparison with SDS-PAGE. Raw, pasteurized, processed at an ultra-high temperature (UHT), and powdered milk samples received increasing concentrations of cheese whey (0, 1, 2.5, 5, 10, 20, 30, 50, and 100% v/v), and were subjected to lab-on-a-chip electrophoresis and SDS-PAGE to detect their mixtures. The lab-on-a-chip methodology was able to separate and quantify milk proteins. In addition, the tested technique is easy, rapid, sensitive, and can detect the addition of cheese whey in milk from the lowest level tested (1%) for milk proteins α-casein and β-casein.
{"title":"Separation and quantification of milk proteins with the addition of cheese whey by lab-on-a-chip","authors":"A. S. O. Santos, H. P. Pereira, G. N. Fogaça, V. M. Meurer, M. Furtado, Cristiano Amâncio Vieira Borges, M. Weller, M. Martins","doi":"10.1590/s1678-3921.pab2023.v58.03099","DOIUrl":"https://doi.org/10.1590/s1678-3921.pab2023.v58.03099","url":null,"abstract":"Abstract The objective of this work was to evaluate microfluidic chip electrophoresis, known as lab-on-a-chip technique, for the detection of milk adulteration using cheese whey in comparison with SDS-PAGE. Raw, pasteurized, processed at an ultra-high temperature (UHT), and powdered milk samples received increasing concentrations of cheese whey (0, 1, 2.5, 5, 10, 20, 30, 50, and 100% v/v), and were subjected to lab-on-a-chip electrophoresis and SDS-PAGE to detect their mixtures. The lab-on-a-chip methodology was able to separate and quantify milk proteins. In addition, the tested technique is easy, rapid, sensitive, and can detect the addition of cheese whey in milk from the lowest level tested (1%) for milk proteins α-casein and β-casein.","PeriodicalId":19826,"journal":{"name":"Pesquisa Agropecuaria Brasileira","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67610076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-29DOI: 10.1590/s1678-3921.pab2023.v58.03126
A. Lazzari, J. C. Corrêa, M. M. Higarashi
Abstract The objective of this work was to evaluate the use of bentonite as an additive in the composting of wastewater sludge from the poultry agroindustry regarding nitrogen conservation, carbon degradation, and nutrient content increase. The experimental design was completely randomized with five treatments with bentonite at 0, 0.5, 1.0, 3.0, and 6.0%, with three replicates. The organic compost used in the treatments consisted of pine sawdust and wastewater sludge from the treatment plant. During and after the composting process, the following were analyzed, respectively: pH, dry matter, temperature, and C, N, NH4+, NO2-, and NO3- contents; and P, K, Ca, Mg, Cu, and Zn contents and humic and fulvic acid concentrations. Bentonite at 6.0% increased C degradation, N losses, Mg content, and humification, but decreased Zn content and humidity. Bentonite, as an additive in the composting of sludge from the poultry agroindustry, promotes humification, decreases Zn content and humidity, but does not affect pH and P, K, Cu, NH4+, NO2-, and NO3- contents.
{"title":"Bentonite as an additive in the composting of wastewater sludge from the poultry agroindustry","authors":"A. Lazzari, J. C. Corrêa, M. M. Higarashi","doi":"10.1590/s1678-3921.pab2023.v58.03126","DOIUrl":"https://doi.org/10.1590/s1678-3921.pab2023.v58.03126","url":null,"abstract":"Abstract The objective of this work was to evaluate the use of bentonite as an additive in the composting of wastewater sludge from the poultry agroindustry regarding nitrogen conservation, carbon degradation, and nutrient content increase. The experimental design was completely randomized with five treatments with bentonite at 0, 0.5, 1.0, 3.0, and 6.0%, with three replicates. The organic compost used in the treatments consisted of pine sawdust and wastewater sludge from the treatment plant. During and after the composting process, the following were analyzed, respectively: pH, dry matter, temperature, and C, N, NH4+, NO2-, and NO3- contents; and P, K, Ca, Mg, Cu, and Zn contents and humic and fulvic acid concentrations. Bentonite at 6.0% increased C degradation, N losses, Mg content, and humification, but decreased Zn content and humidity. Bentonite, as an additive in the composting of sludge from the poultry agroindustry, promotes humification, decreases Zn content and humidity, but does not affect pH and P, K, Cu, NH4+, NO2-, and NO3- contents.","PeriodicalId":19826,"journal":{"name":"Pesquisa Agropecuaria Brasileira","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67610365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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