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Antimicrobial activity of products based on potassium monopersulfate on bacteria associated with avian infections 过硫酸钾产品对禽类感染相关细菌的抑菌活性研究
4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-01-01 DOI: 10.1590/s1678-3921.pab2023.v58.03127
Miguel Augusto Moraes, Mariana Oliveira-Silva, Rafael Silva Goulart, Manoel Henrique Cintra Gabarra, Carlos Eduardo Saraiva Miranda, Paulo Garcia de Almeida, André Pitondo-Silva
Abstract The objective of this work was to evaluate the antimicrobial activity of four new potassium monopersulfate-based products on bacterial biofilms associated with avian infections, in order to disinfect drinking fountains in poultry farms. Initially, tests were performed in planktonic bacterial cells, to verify the antimicrobial activity and the minimum inhibitory concentration of the products, named PA, PB, PC, and PD. These products were tested on mature biofilms of the avian pathogens Escherichia coli, Salmonella enterica subsp. enterica serovar Enteritidis, and Listeria monocytogenes, grown on specimens preserved in acrylic blocks, by counting colony-forming units, scanning electron microscopy, and confocal fluorescence microscopy. All products were effective against the evaluated bacterial species. The PA and PB products inhibited the bacterial growth at ≤ 0.13% concentrations, and PC and PD showed the same effect at ≤ 0.25% concentrations. Furthermore, the PA product was able to eliminate mature biofilms of S. enterica subsp. enterica serovar Enteritidis and L. monocytogenes. The evaluated monopersulfate-based products, notably PA, are effective against bacterial biofilms associated with avian infections and show potential as sanitizers and disinfectants for drinking fountains in poultry farms.
摘要本研究旨在评价四种新型单过硫酸钾基产品对禽类感染相关细菌生物膜的抑菌活性,以期对家禽养殖场饮水机进行消毒。最初,实验在浮游细菌细胞中进行,以验证抗菌活性和产品的最低抑制浓度,命名为PA, PB, PC和PD。这些产品在禽致病菌大肠杆菌、肠沙门氏菌亚种的成熟生物膜上进行了检测。通过计数菌落形成单位,扫描电子显微镜和共聚焦荧光显微镜,在丙烯酸块保存的标本上生长的血清型肠炎肠杆菌和单核增生李斯特菌。所有产品对所评价的细菌种类均有效。PA和PB产物在≤0.13%的浓度下抑制细菌生长,PC和PD在≤0.25%的浓度下抑制效果相同。此外,PA产物还能消除肠链球菌的成熟生物膜。血清型肠炎和单核增生乳杆菌。所评估的以过硫酸盐为基础的产品,特别是PA,对与禽类感染有关的细菌生物膜有效,并显示出作为家禽养殖场饮水机消毒剂的潜力。
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引用次数: 0
Modeling the yield of winter maize using biomass distribution index in the tropical region of Yunnan, China 利用生物量分布指数模拟云南热带地区冬玉米产量
4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-01-01 DOI: 10.1590/s1678-3921.pab2023.v58.03221
Xinyuan Han, Chen Yang, Xiao Weihua, Jie Zhou, Wenfeng Li
Abstract The objective of this work was to establish and validate the dry matter distribution and yield prediction models based on physiological developmental timing, to compare the differences between the dry mass distribution index model and the dry mass distribution coefficient model, for the simulation of ear dry mass and to improve the accuracy of maize growth models for predicting yield. The experiments were conducted in three tropical sites (Longchuan, Mangshi, and Ruili) in the tropical region of Yunnan Province, China. The NRMS of ear dry mass and yield were generally less than 10. The dry mass distribution index method (NRMS = 5.44% and RMSE = 807.22 kg ha-1 for ear dry mass; and NRMS = 7.32% and RMSE = 707.67 kg ha-1 for grain yield) is better than the dry mass distribution coefficient method (NRMS = 7.52% and RMSE = 1115.31 kg ha-1 for ear dry mass; NRMS = 8.6% and RMSE = 830.76 kgha-1 for grain yield) to simulate maize ear dry mass and grain yield. The distribution index model improves the accuracy of the model, which is valuable for future maize production and management in Yunnan.
摘要本研究旨在建立并验证基于生理发育时间的玉米干物质分布和产量预测模型,比较干质量分布指数模型与干质量分配系数模型之间的差异,用于玉米穗干质量的模拟,提高玉米生长模型预测产量的准确性。试验在云南省热带地区的龙川、芒市和瑞丽三个热带站点进行。穗干质量和产量的NRMS均小于10。耳干质量分布指数法(NRMS = 5.44%, RMSE = 807.22 kg ha-1);籽粒产量的NRMS = 7.32%, RMSE = 707.67 kg ha-1)优于干质量分配系数法(穗干质量的NRMS = 7.52%, RMSE = 1115.31 kg ha-1);NRMS = 8.6%, RMSE = 830.76 kga -1(产量),模拟玉米穗干质量和产量。该分布指数模型提高了模型的准确性,对云南未来玉米生产经营具有一定的参考价值。
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引用次数: 0
Lipid profile of artisanal Minas cheese from certified regions in the state of Minas Gerais, Brazil 来自巴西米纳斯吉拉斯州认证地区的手工米纳斯吉拉斯州奶酪的脂质特征
4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-01-01 DOI: 10.1590/s1678-3921.pab2023.v58.03212
Adélia Ferreira Dargère, Sandra Maria Pinto, Jonas Guimarães e Silva, Cesar Augusto Pospissil Garbossa, Diogo Santos Batista, Laryssa Fernandes Correia, Joanna Oliveira Marçal, Peter Bitencourt Faria
Abstract The objective of this work was to determine the fatty acid profile of artisanal Minas cheese from seven certified regions in the state of Minas Gerais, Brazil. A total of 78 samples were collected in regions where the producers were registered by Instituto Mineiro de Agropecuária. For lipid profile determination, different cheese varieties from the following regions were analyzed: Canastra, Serro, Araxá, Serra do Salitre, Triângulo Mineiro, Campo das Vertentes, and Cerrado. The lipid profile of Serra do Salitre and Araxá cheeses was similar in caproic acid (C6:0), total polyunsaturated fatty acids, and ratio of polyunsaturated and saturated fatty acids. In the other regions, lipid profile differed due to associations with specific fatty acids, such as those of the cheeses from Triângulo Mineiro with C20:5n3, of Cerrado with C20:4n6, of Canastra with C8:0, of Serro with C22:2 and C22:6n3, and of Campo das Vertentes with C17:0, C18:0, and C20:0. The fatty acid profile and nutritional indices related to the lipid profile of artisanal Minas cheeses differ according to the region of origin. The content and profile of omega 3 fatty acids are significant parameters to differentiate artisanal Minas cheeses. Serro artisanal Minas cheese presents superior nutritional indices concerning fatty acid profile than the other studied cheeses.
摘要:本研究的目的是测定巴西米纳斯吉拉斯州七个认证地区的手工米纳斯吉拉斯州奶酪的脂肪酸谱。在生产者由Mineiro de Instituto Agropecuária登记的地区共收集了78个样本。为了测定脂质谱,分析了来自以下地区的不同奶酪品种:Canastra、Serro、arax、Serra do Salitre、tri ngulo Mineiro、Campo das Vertentes和Cerrado。Serra do Salitre和arax奶酪的脂质谱在己酸(C6:0)、总多不饱和脂肪酸和多不饱和脂肪酸与饱和脂肪酸的比值方面相似。在其他地区,由于与特定脂肪酸的关联,脂质谱有所不同,例如tri ngulo Mineiro的奶酪与C20:5n3有关,塞拉多的与C20:4n6有关,Canastra的与C8:0有关,Serro的与C22:2和C22:6n3有关,Campo das Vertentes的与C17:0, C18:0和C20:0有关。手工米纳斯奶酪的脂肪酸分布和与脂质分布相关的营养指标因产地而异。omega - 3脂肪酸的含量和分布是区分手工米纳斯奶酪的重要参数。Serro手工米纳斯奶酪在脂肪酸分布方面的营养指标优于其他奶酪。
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引用次数: 0
2NVS translocation presence and inheritance of resistance to head blast in wheat 小麦穗瘟病抗性的nvs易位存在与遗传
4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-01-01 DOI: 10.1590/s1678-3921.pab2023.v58.03038
Camila Vancini, Gisele Abigail Montan Torres, Luciano Consoli, João Leodato Nunes Maciel, Ricardo Lima de Castro, Natália Forchezato Webber, Diógenes Cecchin Silveira, Carolina Cardoso Deuner
Abstract The objective of this work was to evaluate the effect of the presence of 2NVS translocation on the inheritance of wheat resistance to head blast, in F2 segregating lines from two biparental crosses. The IPF 86766 and 'Santa Fe' genotypes were used as female parents (both 2NVS carriers), and 'BRS 404', as the common male parent (non 2NVS carrier). Using a mixture of three isolates of Pyricularia oryzae Triticum, wheat spikes and rachises were evaluated for bleaching severity at five (%BS_5dpi) and seven days post-inoculation (%BS_7dpi), as well as for the number of infection points (IPR) and pathogen sporulation (SPO). Chi-square analyses showed that segregation follows a 3:1 Mendelian hypothesis, with a major locus with a dominant allele: %BS_7dpi for IPF 86766 × 'BRS 404' and IPR for 'Santa Fe' x 'BRS 404'. Therefore, this is the first known study to show that the 2NVS translocation responsible for resistance to head blast is a major locus with a dominant allele. In a controlled environment, 'BRS 404' shows bleaching severity and SPO similar with those occurring on IPF 86766 and 'Santa Fe'.
摘要本研究旨在评价2NVS易位对两个双亲本杂交F2分离系小麦抗穗瘟病遗传的影响。用IPF 86766和‘Santa Fe’基因型作为母本(均为2NVS携带者),用‘BRS 404’基因型作为普通父本(非2NVS携带者)。利用稻瘟病菌(pyricaria oryzae Triticum) 3个分离株的混合物,对接种后5天(%BS_5dpi)和7天(%BS_7dpi)小麦穗和小麦穗的白化严重程度、侵染点(IPR)数量和病原菌孢子量(SPO)进行了评价。卡方分析表明,分离符合3:1孟德尔假设,一个主位点有一个显性等位基因:IPF 86766 בBRS 404’的%BS_7dpi和‘Santa Fe’בBRS 404’的IPR。因此,这是已知的第一个研究表明,负责头部爆炸抗性的2NVS易位是一个显性等位基因的主要位点。在受控环境下,“BRS 404”显示出与IPF 86766和“Santa Fe”类似的漂白严重程度和SPO。
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引用次数: 0
Interrelationship between potassium rates and the efficiency of Bt genes in the control of Spodoptera frugiperda 施钾率与Bt基因防治夜蛾效率的相互关系
4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-01-01 DOI: 10.1590/s1678-3921.pab2023.v58.03241
Marcelo Macedo Faria, Pedro Henrique Nascimento Cintra, Víctor Alves Amorim, Thiago Souza Campos, Ednaldo Cândido Rocha, Fabricio Rodrigues
Abstract The objective of this work was to identify the effect of potassium rates on Bt transgenic resistance and damage caused by Spodoptera frugiperda in corn plants at different developmental stages. The experimental design was a randomized complete block in a 3x6 factorial arrangement, with three potassium rates (0, 45, and 90 kg ha-1) and six corn hybrids (20A55Hx, 30A77PW, AG1051, MG652PW, NS90VTPro2, and NS92VTPro), with three replicates. The hybrids were evaluated through the analysis of canonical variables. With low potassium rates, the effectiveness of the corn hybrids in controlling the S. frugiperda caterpillar decreases in the different phenological stages. The use of the Bt technology is more efficient under an adequate potassium rate of 90 kg ha-1 KCl.
摘要本研究旨在研究施钾量对不同发育阶段玉米对Bt转基因玉米的抗性及穗状夜蛾危害的影响。试验设计采用3 × 6因子随机完全区组设计,采用3个钾浓度(0、45和90 kg ha-1)和6个玉米杂交品种(20A55Hx、30A77PW、AG1051、MG652PW、NS90VTPro2和NS92VTPro), 3个重复。通过典型变量分析对杂种进行评价。施钾量较低时,玉米杂交种防治金翅虫的效果在不同物候阶段均呈下降趋势。在90 kg ha-1 KCl的适钾量下,Bt技术的使用效率更高。
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引用次数: 1
Temporal dynamics of Plasmopara viticola as function of bud load increase in 'Sauvignon Blanc' 白苏维浓(Sauvignon Blanc)芽量增加对葡萄浆原菌时间动态的影响
4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-01-01 DOI: 10.1590/s1678-3921.pab2023.v58.03224
Douglas André Wurz, Alberto Fontanella Brighenti, Ricardo Allebrandt, Leo Rufato
Abstract The objective of this work was to evaluate the effect of increasing bud load per plant on epidemiological variables of downy mildew on 'Sauvignon Blanc'. The study was carried out in a commercial vineyard in a high-altitude region of the municipality of São Joaquim, in the state of Santa Catarina, Brazil, during the 2016/2017 and 2017/2018 vintages. The treatments consisted of four bud loads: 15, 30, 50, and 75 per vine. The maximum incidence, maximum severity, beginning of symptom appearance, time to reach maximum disease incidence and severity, area under the incidence and severity disease progress curve were determined. Increasing bud load over 50 per vine results in higher incidence and severity of downy mildew (Plasmopara viticola) in 'Sauvignon Blanc' leaves but does not influence the time to reach maximum disease incidence and severity. Increasing bud load over 50 per vine results in an increase in the area under the disease incidence and severity progress curve for downy mildew in 'Sauvignon Blanc' leaves. To adopt over 50 buds per vine treatment, it is necessary additional disease control measures, with an emphasis on integrated management to control downy mildew.
摘要本研究旨在评价增加单株芽量对长相思霜霉病流行病学变量的影响。该研究于2016/2017和2017/2018年份在巴西圣卡塔琳娜州s o Joaquim市高海拔地区的一个商业葡萄园进行。处理包括4个芽负荷:每株15、30、50和75个。测定最大发病率、最大严重程度、症状开始出现、达到最大发病率和严重程度的时间、发病率和严重程度疾病进展曲线下的面积。每棵葡萄增加50个以上的芽负荷会导致白苏维翁叶片霜霉病的发病率和严重程度增加,但不影响达到最高发病率和严重程度的时间。当每株葡萄增加50个以上的芽量时,长相思白霜霉病发病和严重程度进展曲线下的面积就会增加。采用每株50芽以上的处理,需要额外的疾病控制措施,重点是综合管理,以控制霜霉病。
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引用次数: 0
Growth promotion in different Urochloa species inoculated with Azospirillum baldaniorum 接种氮螺旋菌对不同种类尿藻生长的促进作用
4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-01-01 DOI: 10.1590/s1678-3921.pab2023.v58.03079
Liliandra Barreto Emídio Gomes, Thamires Ferreira Rodrigues da Silva, Gabriela Cavalcanti Alves, Veronica Massena Reis
Abstract The objective of this work was to evaluate inoculation response to Azospirillum baldaniorum strain Sp245 in 14 Urochloa cultivars. The evaluated cultivars were: Marandú, Xaraés, Paiaguás, Piatã, Basilisk, B140, Comum, Tupi, H47, Llanero, Ruziziensis, Ipyporã, Mulato, and Convert HD364. Three experiments were conducted to measure root traits: two using soil and one a sterile substrate. Compared with the control, a single application of A. baldaniorum Sp245 increases dry matter accumulation and number of roots in cultivars Marandú, Llanero, Ruziziensis, and Convert HD364. However, after inoculation, cultivars Xaraés and Ipyporã show some root traits with lower values.
摘要本研究旨在评价14个尿毒草品种对baldanorum Azospirillum Sp245的接种反应。评价品种为:Marandú、xara、Paiaguás、Piatã、Basilisk、B140、Comum、Tupi、H47、Llanero、Ruziziensis、Ipyporã、Mulato和Convert HD364。进行了三个试验来测量根系性状:两个用土壤,一个用无菌基质。与对照相比,单次施用baldanorum Sp245增加了Marandú、Llanero、Ruziziensis和Convert HD364的干物质积累和根系数量。但接种后,xarasamass和Ipyporã品种的一些根系性状值较低。
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引用次数: 0
Genetic parameters and validation of microsatellite markers associated with iron and zinc in common bean 蚕豆铁、锌相关微卫星标记的遗传参数及验证
4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-01-01 DOI: 10.1590/s1678-3921.pab2023.v58.03267
Poliana Regina Carloni, Thiago Lívio Pessoa Oliveira de Souza, Marcelo Sfeir de Aguiar, Leonardo Cunha Melo, Patrícia Guimarães Santos Melo, Helton Santos Pereira
Abstract The objective of this work was to estimate the genetic parameters, evaluate the agronomic performance, and validate the microsatellite molecular markers (SSRs) linked with quantitative trait loci (QTLs) for Fe and Zn concentrations in grains of common bean, in order to select superior lines. One hundred and sixteen lines from two populations ('BRS Requinte' × 'Porto Real' and 'BRS Requinte' × G2358) and five check genotypes were evaluated in three environments. The parents and lines were genotyped with 20 SSRs. In the simultaneous selection of the lines for the four evaluated traits, the gains from selection were 4.7% for Fe concentration, 2.8% for Zn concentration, 3.9% for yield, and 0.9% for 100-seed weight. Therefore, there is the possibility of selection of lines that combine desirable phenotypes for the traits of interest. The only polymorphic marker is BM 154 in the 'BRS Requinte' × 'Porto Real' population, indicating that the QTLs linked with the markers may already be fixed or that the markers are not associated in the used populations. The single-marker analysis of QTL mapping shows an association between BM 154 and Fe concentration in only one environment, explaining 14.5% of phenotypic variation, which indicates the occurrence of the interaction of QTLs with environments.
摘要本研究的目的是估算普通豆籽粒铁和锌含量的遗传参数,评价其农艺性能,并验证与数量性状位点(qtl)连锁的微卫星分子标记(SSRs),以选择优质品系。在3种环境中对来自2个群体(‘BRS Requinte’בPorto Real’和‘BRS Requinte’× G2358)的116个品系和5个检查基因型进行了评价。用20个ssr对亲本和品系进行基因分型。在4个评价性状的同时选育中,铁浓度、锌浓度、产量和百粒重分别获得4.7%、2.8%、3.9%和0.9%的增益。因此,有可能选择结合感兴趣性状的理想表型的系。在“BRS Requinte”דPorto Real”群体中,唯一的多态性标记是bm154,这表明与这些标记关联的qtl可能已经固定,或者这些标记在使用的群体中没有关联。QTL定位的单标记分析显示,bm154与铁浓度仅在一种环境中存在关联,解释了14.5%的表型变异,这表明QTL与环境存在相互作用。
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引用次数: 0
Cover plants with potential to reduce two-spotted spider mite population in soybean 覆盖有潜力减少大豆双斑蜘蛛螨种群的植物
4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-01-01 DOI: 10.1590/s1678-3921.pab2023.v58.03332
Luana Cristina Alves Ribeiro, Felipe Lemos, Dalila Dominique Duarte Rocha, Marcos Antônio Matiello Fadini
Abstract The objective of this work was to evaluate the potential of cover plants in reducing the population of the two-spotted spider mite, Te t r a n y c h u s urticae, in soybean. Seven host plants – cotton (Gossypium hirsutum), Urochloa ruziziensis, crotalaria (Crotalaria juncea), corn (Zea mays), radish (Raphanus sativus), soybean (Glycine max), and sorghum (Sorghum bicolor) – were evaluated for their effect on T. urticae biological parameters (oviposition, development stages, and survival) and preference for volatile odors (using a Y-tube olfactometer). Tetranychus urticae preferred cotton and showed a higher population growth rate on this host plant. The survival and oviposition of T. urticae was prolonged in radish and crotalaria plants, whereas juveniles did not complete their immature development on U. ruziziensis leaves. No preference was observed between the volatile odors of U. ruziziensis, radish, and crotalaria. Urochloa ruziziensis can reduce T. urticae populations in soybean plants.
摘要本研究旨在评价覆盖植物对大豆双斑蜘蛛螨种群的抑制作用。研究了棉花(Gossypium hirsutum)、ruziziensis、crotalaria juncea (crotalaria juncea)、玉米(Zea mays)、萝卜(Raphanus sativus)、大豆(Glycine max)和高粱(sorghum bicolor)等7种寄主植物对荨麻粉虱生物学参数(产卵、发育阶段和存活)和对挥发性气味的偏好(使用y管嗅觉仪)的影响。荨麻叶螨偏爱棉花,在棉花上表现出较高的种群增长率。在萝卜和甘蓝植株上,荨麻疹夜蛾的存活和产卵时间较长,而幼虫在紫荆叶片上发育不完全。红萝卜挥发性气味与红萝卜挥发性气味没有明显的差异。褐藻可减少大豆植株上的荨麻疹菌种群。
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引用次数: 0
Management of blackberry pruning to extend harvest seasonality 管理黑莓修剪延长收获季节
4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-01-01 DOI: 10.1590/s1678-3921.pab2023.v58.03197
Gabriel Laquete de Barros, Rafael Pio, Carlos Henrique Milagres Ribeiro, Lucídio Henriques Vote Fazenda, Alexandre Dias da Silva, Pedro Maranha Peche
Abstract The objective of this work was to evaluate the pruning management of the BRS Tupy and Brazos blackberry cultivars, in order to extend their harvest seasonality and fruit yield. The experiment was performed in an altitude subtropical region in the state of Minas Gerais, Brazil. Bushes were grown at 3.0x0.5 m spacing. The treatments consisted of the four following pruning seasons: one conventional pruning, with suppression of the produced stems in February and reduction of the stems in July; and three drastic pruning performed in the first two weeks of January, March, and May, with the application of 10% urea and 3% hydrogenated cyanamide five months later. The experimental design was in randomized complete blocks, in a 2x4 factorial arrangement, with two cultivars and four pruning managements, with four blocks. Fruit phenology, yield, and physicochemical quality were evaluated in two production cycles. It is possible to extend the harvest season of blackberry up to five months, in an altitude subtropical region in Lavras, in the state of Minas Gerais, Brazil, with drastic pruning in January and no irrigation. Drastic pruning in January or March increases the yield of the BRS Tupy and Brazos blackberry cultivars. The extended harvest season does not affect the quality of blackberries and does not create challenges for harvesting operations.
摘要为了延长黑莓品种的采收季节和果实产量,对BRS Tupy和Brazos两种黑莓品种的修剪管理进行了评价。实验是在巴西米纳斯吉拉斯州的亚热带高原地区进行的。灌木以3.0 × 0.5 m的间距生长。处理包括以下四个修剪季节:一个常规修剪,2月抑制产出茎,7月减少茎;在1月、3月和5月的前两周进行3次大幅度修剪,5个月后施用10%尿素和3%氢化氰酰胺。试验设计为随机完整区,2 × 4因子排列,2个品种和4个修剪管理,4个区。在两个生产周期内对果实物候、产量和理化品质进行了评价。在巴西米纳斯吉拉斯州拉夫拉斯(Lavras)的亚热带高原地区,黑莓的收获季节可以延长至5个月,在1月份进行剧烈的修剪,没有灌溉。1月或3月的剧烈修剪增加了BRS Tupy和Brazos黑莓品种的产量。延长的收获季节不会影响黑莓的质量,也不会给收获作业带来挑战。
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引用次数: 0
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Pesquisa Agropecuaria Brasileira
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