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Genetic diversity of Xanthomonas phaseoli p v. manihotis populations using rep-PCR and VNTR molecular markers 利用rep-PCR和VNTR分子标记分析相黄单胞菌的遗传多样性
4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-01-01 DOI: 10.1590/s1678-3921.pab2023.v58.03299
Laís Barreto de Oliveira, Saulo Alves Santos de Oliveira, Ricardo Franco Cunha Moreira, Maria Selma Alves Silva Diamantino, Andresa Priscila de Souza Ramos, Taliane Leila Soares, Claudia Fortes Ferreira
Abstract The objective of this work was to evaluate the genetic diversity of Xanthomonas phaseoli p v. manihotis (Xpm) from eight populations from five cassava producing states in Brazil, through the rep-PCR (BOX-PCR and ERIC-PCR) and variable number of tandem repeat (VNTR) markers. Cassava leaves with symptoms of cassava bacterial blight were collected in eight municipalities, and the Xpm isolates were identified by amplification with primers specific for these isolates. The identity of the Xpm isolates was confirmed with the BOX-PCR, ERIC-PCR, and VNTR markers. The observed selection pressure, together with the mode of reproduction and the mechanisms that increase genetic variability, allows of the pathogen populations to adapt according to microclimate variation, contributing to a differentiated reproductive success. ERIC-PCR and VNTRs are the best markers for evaluating the genetic variability in the eight studied Xpm populations. However, ERIC-PCR is the marker that best separated the groups by population and presented a higher similarity between the isolates of the same population. The study of the genetic diversity of Xpm is key to improve disease monitoring and management strategies in cassava crops.
摘要采用BOX-PCR、ERIC-PCR和可变数目串联重复序列(VNTR)标记,对巴西5个木薯产地8个群体的相黄单胞菌(Xanthomonas phaseoli p . v. manihotis, Xpm)进行遗传多样性分析。在8个城市收集了具有木薯细菌性枯萎病症状的木薯叶片,用这些菌株的特异性引物扩增鉴定了Xpm分离株。采用BOX-PCR、ERIC-PCR和VNTR标记对Xpm分离株进行鉴定。观察到的选择压力,加上繁殖模式和增加遗传变异的机制,使病原体种群能够根据小气候的变化进行适应,从而有助于差异化的繁殖成功。ERIC-PCR和VNTRs是评价所研究的8个Xpm群体遗传变异的最佳标记。而ERIC-PCR是用群体区分群体效果最好的标记,且同一群体的分离物具有较高的相似性。研究Xpm的遗传多样性是提高木薯病害监测和管理策略的关键。
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引用次数: 0
Photosynthetic response of citrus to Citrus tristeza virus isolates with potential for cross-protection 柑橘对具有交叉保护潜力的柑橘tristeza病毒分离株的光合反应
4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-01-01 DOI: 10.1590/s1678-3921.pab2023.v58.03290
Rosalba Contreras-Maya, Angel Villegas-Monter, Nicacio Cruz-Huerta, Amalio Santacruz-Varela, Laura D. Ortega-Arenas, Daniel-Leobardo Ochoa-Martínez
Abstract The objective of this work was to evaluate the photosynthetic response of citrus varieties to the inoculation of isolates T30 and T3 of Citrus tristeza virus (CTV) characterized as having potential to provide cross-protection against severe CTV isolates in citrus. Citrus plants of 'Campbell' orange, 'Persian' lime, and 'Key' lime were subjected to inoculations by both isolates by patch. Noninoculated plants were used as the control. Symptom expression, photosynthetic and transpiration rates, and stomatal conductance were evaluated by IRGA. Stomatal density and size were evaluated by epidermal impressions. The CTV isolates were also evaluated and molecularly characterized by RT-PCR and Sanger sequencing. 'Campbell' orange and 'Persian' lime plants remained asymptomatic after the inoculations. The inoculation treatments with both CTV isolates did not reduce photosynthetic capacity, transpiration rate, and stomatal conductance, in comparison with the control. Stomatal density and size varied according to the citrus species. T30 and T3 do not affect the photosynthetic responses of 'Campbell' orange and 'Persian' lime; therefore, these isolates have the potential to provide cross-protection to citrus varieties against severe isolates of CTV.
摘要本研究旨在评价柑橘品种对接种柑橘tristeza病毒(CTV)分离株T30和T3的光合反应,该分离株具有交叉保护柑橘严重CTV分离株的潜力。对‘Campbell’橙、‘波斯’酸橙和‘Key’酸橙柑橘植株进行了贴片接种。以未接种植株为对照。用IRGA评价症状表达、光合速率和蒸腾速率以及气孔导度。通过表皮印痕评价气孔密度和大小。通过RT-PCR和Sanger测序对CTV分离株进行了评价和分子表征。“坎贝尔”橙和“波斯”酸橙在接种后仍无症状。与对照相比,接种两种CTV菌株均未降低光合能力、蒸腾速率和气孔导度。不同种类柑橘的气孔密度和大小各不相同。T30和T3不影响‘坎贝尔’橙和‘波斯’青柠的光合反应;因此,这些分离株有可能为柑橘品种提供抗CTV严重分离株的交叉保护。
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引用次数: 0
Supplementation with lipid sources in diets for Jersey cows in the first third of lactation 泌乳前三分之一泽西奶牛饲粮中添加脂质源的研究
4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-01-01 DOI: 10.1590/s1678-3921.pab2023.v58.03149
Ana Paula Binato de Souza, Jorge Schafhauser Junior, Rudolf Brand Scheibler, Ana Carolina Fluck, Fábio Antunes Rizzo, José Laerte Nörnberg, Diego Prado de Vargas, Lívia Argoud Lourenço
Abstract The objective of this work was to evaluate the effect of the addition of whole-crude oilseeds of linseed, sunflower, and soybean to the diet of Jersey cows, in the first third of lactation, on the following characteristics: intake, apparent nutrient digestibility, milk production and its variables, energy-corrected milk, feed efficiency, energy balance, milk fatty acid profile, and blood metabolic profile. Eight multiparous Jersey cows were used in a double Latin square design. The treatments were the experimental diets with the oilseeds, and calcium salts of fatty acids were used as the control. Protein digestibility was lower for soybean, whereas that of ether extract was higher in the control, not differing between the evaluated oilseeds. There was no difference between oilseeds for milk production, milk production corrected for fat, milk nutritional content, feed efficiency, energy balance, and blood metabolic profile. The levels of mil k monou n sat u rated fatty acids increased with the use of sunflower. Higher levels of cis-9, trans-11 conjugated linoleic acid (CLA) in milk were observed in the control. The addition of the evaluated whole-crude oilseeds to the diet of Jersey cows in the first third of lactation does not affect milk production variables, feed efficiency, energy balance, and blood biochemical profile, but alters the fatty acid profile.
摘要本试验旨在评价泌乳期前三分之一泽西奶牛饲粮中添加亚麻籽、葵花籽和大豆全粗油籽对采食量、营养物质表观消化率、产奶量及其变量、能量修正乳、饲料效率、能量平衡、乳脂肪酸谱和血液代谢谱的影响。8头产多产泽西奶牛采用双拉丁方设计。以油籽为试验饲料,脂肪酸钙盐为对照。大豆的蛋白质消化率较低,而对照组的脂肪提取物的蛋白质消化率较高,在评价的油籽之间没有差异。在产奶量、经脂肪校正的产奶量、乳营养成分、饲料效率、能量平衡和血液代谢谱方面,油籽之间没有差异。随着葵花的使用,牛奶中脂肪酸和脂肪酸的含量增加。在对照组中观察到牛奶中较高水平的顺式-9,反式-11共轭亚油酸(CLA)。在泌乳期前三分之一的泽西奶牛饲粮中添加所评价的全粗油籽,对泌乳指标、饲料效率、能量平衡和血液生化特征没有影响,但改变了脂肪酸特征。
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引用次数: 0
Maternal effect on thermotolerance in lettuce seeds 母系对莴苣种子耐热性的影响
4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-01-01 DOI: 10.1590/s1678-3921.pab2023.v58.03298
Pedro Yuri Cavasin, Luiz Antônio Augusto Gomes, Wilson Vicente Souza Pereira, Heloisa Oliveira dos Santos
Abstract The objective of this work was to evaluate the occurrence of the maternal effect on thermotolerance in lettuce (Lactuca sativa) seeds, as well as to find an enzymatic marker for this trait in lettuce seedlings. Seeds from the Everglades thermotolerant cultivar, the Verônica susceptible cultivar, and their reciprocal hybrids were used. For each cultivar and reciprocal hybrid, germination percentage and vigor (germination speed index) tests were carried out at 20 and 32°C. Thermotolerance was defined as the capacity to germinate at 32°C. To obtain markers that could be associated with thermotolerance, enzymatic tests were performed. The bands of the electrophoretic enzymatic extracts were quantified using the ImageJ software. The hybrids behaved similarly to their female genitors. When cultivar Verônica was the female genitor, hybrid seeds did not germinate at high temperatures and showed a lower expression of the esterase enzyme. However, when the female genitor was the Everglades cultivar, hybrids showed a high germination at high temperatures and a greater esterase expression. Therefore, there is a maternal effect on tolerance to thermoinhibition in lettuce seeds, and the esterase enzyme can be used as a selective marker to identify homozygous progenies for this trait.
摘要本研究旨在探讨母系对莴苣种子耐热性的影响,并寻找莴苣幼苗耐热性的酶标记。选用Everglades耐热品种、Verônica易感品种及其互交杂交种的种子。在20℃和32℃条件下,对各品种和互交杂交种进行发芽率和活力(萌发速度指数)试验。耐热性定义为在32℃下发芽的能力。为了获得可能与耐热性相关的标记,进行了酶促试验。用ImageJ软件对电泳酶提物的条带进行定量分析。这些杂交后代的行为与它们的雌性后代相似。当品种Verônica为雌性时,杂交种子在高温下不发芽,酯酶的表达也较低。然而,当母系为Everglades品种时,杂种在高温下萌发率高,酯酶表达量高。因此,莴苣种子对热抑制的耐受性存在母系效应,酯酶可作为鉴定该性状纯合后代的选择标记。
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引用次数: 0
Data from NASA Power and surface weather stations under different climates on reference evapotranspiration estimation NASA Power和地面气象站在不同气候条件下的参考蒸散发估算数据
4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-01-01 DOI: 10.1590/s1678-3921.pab2023.v58.03261
Stefanie Lais Kreutz Rosa, Jorge Luiz Moretti de Souza, Aline Aparecida dos Santos
Abstract The objective of this work was to evaluate the data estimated by NASA Power in relation to that measured at surface weather stations under different climates, and to verify the effects of these data on reference evapotranspiration (ETo) estimation. For comparison, data measured at 21 surface weather stations, located in Brazil, Israel, Australia, Portugal, and the United States of America were used, representing different Köppen climate types. The following climatic variables were analyzed daily: maximum (Tmax), mean (Tmean), and minimum (Tmin) air temperatures; wind speed; incident solar radiation; and mean relative humidity (RHmean). Wind speed showed the highest variations and was overestimated in the Cfb, BWh, BSh, and Cfa climates. Tmean and mean wind speed were estimated accurately in the Csa and BWh climates, whereas Tmax and Tmin were underestimated in 13 and 9 climates, respectively; Tmin did not show adequate results in tropical climates. Incident solar radiation was overestimated in all climates, except in BSh, but presented the best statistical indicators among the analyzed variables. The scenarios in which ETo was estimated using the Penman-Monteith method and data from NASA Power were consistent even for the climate type that presented the worst association between measured and estimated data.
摘要:本研究的目的是将NASA Power估算的数据与不同气候条件下地面气象站的测量数据进行比较,并验证这些数据对参考蒸散发(ETo)估算的影响。为了进行比较,使用了位于巴西、以色列、澳大利亚、葡萄牙和美利坚合众国的21个地面气象站测量的数据,代表了不同的Köppen气候类型。每日分析以下气候变量:最高气温(Tmax)、平均气温(Tmean)和最低气温(Tmin);风速;入射太阳辐射;和平均相对湿度(RHmean)。风速在Cfb、BWh、BSh和Cfa气候中变化最大,且被高估。平均风速和平均风速在Csa和BWh气候中被准确估计,而Tmax和Tmin分别在13个和9个气候中被低估;Tmin在热带气候中没有显示出足够的效果。除BSh外,所有气候条件下的太阳入射辐射均被高估,但在各分析变量中表现出最好的统计指标。使用Penman-Monteith方法估算的ETo情景和来自NASA Power的数据是一致的,甚至对于在测量数据和估算数据之间表现出最坏关联的气候类型也是如此。
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引用次数: 0
Chemical thinning programs for 'Fuji Mishima' apple trees under black anti-hail net “富士三岛”苹果树在黑色防雹网下的化学间伐方案
4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-01-01 DOI: 10.1590/s1678-3921.pab2023.v58.03196
Lucas De Ross Marchioretto, Andrea De Rossi, Gilmar Arduino Bettio Marodin
Abstract The objective of this work was to evaluate chemical thinning programs containing mixtures of different plant growth regulators, at low concentrations, applied to 'Fuji Mishima' apple trees at post-blossom, as well as to identify the most effective in reducing fruit set, decreasing the need of manual thinning, and improving fruit quality. Under a black anti-hail net, the apple trees were sprayed with the six following chemical thinning protocols (treatments): benzyladenine (BA) + gibberellin4+7 (GA) at full bloom (FB) and BA + carbaryl (CB) on fruitlets with a 15 mm diameter; BA + GA at FB and naphthaleneacetic acid (NAA) + CB on fruitlets with a 7 mm diameter; BA + GA at FB and BA + CB on fruitlets with a 15 mm diameter; ethephon (ETH) + CB on fruitlets with a 15 mm diameter and metamitron (MM) + ETH on fruitlets with a 20 mm diameter; and MM on fruitlets with a 7 mm diameter and MM on fruitlets with a 20 mm diameter. The treatments were compared with an untreated and a manual thinning control. The black net reduced photosynthetically active radiation in 22%. For 'Fuji Mishima' apples under a black anti-hail net, the chemical thinning program consisting of BA + GA (47 + 47 g a.i. ha-1) at FB, followed by BA + CB (80 + 288 g a.i. ha-1) on fruitlets with a 15 mm diameter, consistently reduces fruit set, requires less manual thinning, and improves fruit weight and size.
摘要本研究的目的是评价不同植物生长调节剂混合物在“富士三岛”苹果树开花后的化学间伐方案,并确定在减少坐果、减少人工间伐和提高果实质量方面最有效的方案。在黑色防雹网下,对苹果树进行了6种化学间伐处理,分别是在开花时喷苯ladenine (BA) +赤霉素4+7 (GA),在15 mm直径的小果实上喷BA + carviyl (CB);BA + GA在FB和萘乙酸(NAA) + CB在直径为7 mm的小果实上;BA + GA在FB和BA + CB在直径为15mm的小果实上;乙烯利(ETH) + CB涂在直径15mm的水果上,亚胺铁(mm) + ETH涂在直径20mm的水果上;直径为7mm的果实为MM,直径为20mm的果实为MM。将这些处理与未处理和手工减薄对照进行比较。黑网减少了22%的光合有效辐射。对“富士三岛”苹果在黑色防雹网下施用BA + GA (47 + 47 g a.i. ha-1),然后在直径为15 mm的小果实上施用BA + CB (80 + 288 g a.i. ha-1)的化学间伐方案,可以持续减少坐果,减少人工间伐,提高果实的重量和大小。
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引用次数: 0
Development of Israeli mango cultivars in the Brazilian semiarid region 以色列芒果品种在巴西半干旱区的发展
4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-01-01 DOI: 10.1590/s1678-3921.pab2023.v58.03173
Maria Jany Kátia Loiola Andrade, Alexandre Santos de Oliveira, Walber Felix dos Santos, Luan dos Santos Silva, Jenilton Gomes da Cunha, Antônio Gustavo de Luna Souto, Ítalo Herbert Lucena Cavalcante
Abstract The objective of this work was to evaluate the initial adaptive performance of Israeli mango tree cultivars grown in the submedian region of the São Francisco Valley, Brazil. The experiment was carried out from January 2019 to July 2020 using seedlings of the Omer and Shelly cultivars, at six months after transplanting, at a 3×6 m spacing. The experimental design was randomized complete blocks in a 2×3 factorial arrangement, corresponding to the two Israeli mango tree cultivars and the number of branches after formative pruning (three, four, and five branches), with four replicates. Biometric, biochemical, and photosynthetic variables were analyzed, differing between the evaluation times after pruning. The Omer cultivar is more vigorous than Shelly, and formative pruning with three, four, and five branches is recommended for both mango cultivars under the cultivation conditions of the São Francisco Valley.
摘要本研究的目的是评估生长在巴西奥弗朗西斯科山谷亚中部地区的以色列芒果树品种的初始适应性能。该试验于2019年1月至2020年7月在移栽后6个月,以3×6 m的间距进行。试验设计采用2×3因子随机完整区组,分别对应两个以色列芒果树品种和形成剪枝后的枝数(3枝、4枝和5枝),设4个重复。在修剪后的评估时间之间,分析了不同的生物计量学、生化和光合变量。奥梅尔品种比雪莉更有活力,在弗朗西斯科山谷的栽培条件下,两种芒果品种都建议采用三、四、五枝的形成修剪。
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引用次数: 0
Morphological traits of 'Granada' peach nursery trees from rootstocks of two production systems “格拉纳达”桃树苗木两种生产体系的形态特征
4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-01-01 DOI: 10.1590/s1678-3921.pab2023.v58.03225
Guilherme Nicolao, Newton Alex Mayer, Bernardo Ueno, Valmor João Bianchi
Abstract The objective of this work was to evaluate the effect of the use of rootstocks from clonal and seed production systems on the morphology of nursery peach trees of cultivar Granada. The experiment was carried out in a completely randomized design with five treatments ('Granada' scion grafted onto five rootstocks) and five replicates. The rootstocks from clonal production were the 'Okinawa' peach plant and the GKM-ELD-10-70, EF-SAU-10-78, and EF-SAU-10-87 peach genotypes kept in pots. The rootstocks from the seed production system came from peach seed of some scion varieties (mixed pits) used for canning production and propagated in a nursery field system. Shoot and root morphological variables were evaluated. The rootstock production system did not affect root volume. However, the nursery trees of the clonal rootstocks system kept in pots showed a higher percentage of water in the roots. The clonal rootstocks in the potted system do not affect most of the morphological traits of 'Granada' peach nursery trees; however, they induce a homogeneous root system and delay leaf abscission, besides not requiring root pruning.
摘要本研究旨在评价克隆生产体系和种子生产体系的砧木使用对格拉纳达桃树苗木形态的影响。试验采用完全随机设计,5个处理(‘格拉纳达’接穗接在5根砧木上),5个重复。无性系生产的砧木为“冲绳”桃植株和盆栽保存的gkm - ld -10-70、ef - su -10-78和ef - su -10-87桃基因型。制种系统的砧木来自于一些用于罐头生产的接穗品种(混合坑)的桃种子,并在苗圃系统中繁殖。对茎和根的形态变量进行了评价。砧木生产系统对根体积没有影响。而盆栽无性系砧木体系的苗木根系水分含量较高。盆栽体系中的无性系砧木对格拉纳达桃树苗木的大部分形态性状没有影响;然而,除了不需要根修剪外,它们还能诱导均匀的根系并延缓叶片脱落。
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引用次数: 0
Food product preparation from the vinification of 'BRS Violeta' by-products 由'BRS Violeta'副产品酿造的食品制备
4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-01-01 DOI: 10.1590/s1678-3921.pab2023.v58.03119
Tuany Yuri Kuboyama Nogueira, Victoria Diniz Shimizu-Marin, Ana Carolina Gonçales, Heloíza Ferreira Alves do Prado, Roberto da Silva, Ellen Silva Lago-Vanzela
Abstract The objective of this work was to manufacture pizza dough, truffle, and cake using the paste of a by-product from the vinification of 'BRS Violeta' grape, as well as to determine some bromatological characteristics of these products. The by-product was pressed to remove excess must and crushed to obtain the paste. The paste, pasteurized at 80°C for 3 min and frozen at -18°C, was characterized for total phenolic compounds (TPC), total monomeric anthocyanins (TMA), and dietary fiber. The food products were characterized for the following parameters: moisture, energy value, and protein, lipid, ash, dietary fiber, total carbohydrates, and TPC contents. The contents found in the paste were: 326 mg GAE 100 g-1, for TPC; 185 mg mv35diglc 100g-1, for TMA; and 10 g 100 g-1, for dietary fibers. Based on the centesimal compositions of the food products, the pizza dough and truffle can be considered as a source of fiber and protein, respectively. The products show TPC contents between 51 and 63 mg GAE 100 g-1. It is possible to prepare food products of good nutritional quality, using grape by-product paste, to minimize waste in the field.
摘要本研究的目的是利用BRS Violeta葡萄酿造的副产物膏体制作披萨面团、松露和蛋糕,并确定这些产品的一些风味特征。副产物被压去多余的必须和粉碎得到糊状物。经80°C巴氏杀菌3分钟,-18°C冷冻后,膏体的主要成分为总酚类化合物(TPC)、总单体花青素(TMA)和膳食纤维。通过水分、能量值、蛋白质、脂肪、灰分、膳食纤维、总碳水化合物和TPC含量等参数对食品进行表征。膏体中TPC含量为326 mg GAE 100 g-1;185 mg mv35diglc 100g-1,用于TMA;10克100克,是膳食纤维。根据食品的百分成分,比萨饼面团和松露可以分别被认为是纤维和蛋白质的来源。产品TPC含量在51 ~ 63 mg GAE 100 g-1之间。利用葡萄副产品膏可以制备营养质量好的食品,从而最大限度地减少田间浪费。
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引用次数: 0
Sampling sufficiency for estimating the mean of wheat traits 估计小麦性状平均值的抽样充分性
4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-01-01 DOI: 10.1590/s1678-3921.pab2023.v58.03271
Alberto Cargnelutti Filho, Jéssica Maronez de Souza, Ismael Mario Márcio Neu, Daniela Lixinski Silveira, Valéria Escaio Bubans, Samanta Luiza da Costa, Lucas Fillipin Osmari, Bruno Fillipin Osmari
Abstract The objective of this work was to determine the sample size necessary for estimating the means of wheat (Triticum aestivum) traits, obtained through measurement, counting, and weighing. Seventeen uniformity trials were performed with 1,790 plants harvested randomly, whose following traits were evaluated: lengths of the main stem and main stem ear (measurement); number of leaves, stems, and ears (counting); and mass of fresh and dry matter of leaves, stems, and ears (weighing). The Bartlett and Kolmogorov-Smirnov tests and Welch’s analysis of variance were performed. Skewness, central tendency, and variability were determined, and sample size was calculated to estimate the means of the 13 evaluated traits, considering estimation errors (semi-amplitudes of the 95% confidence interval) equal to 5, 10, 15, and 20% of the mean. There is a decrease in the sample size to estimate the means of wheat traits obtained through weighing, counting, and measuring, in this order. In an experiment to estimate the mean of wheat traits obtained by weighing, counting, and measuring with a maximum error of 10% of the mean at a 95% confidence interval, 117, 76, and 9 plants per treatment are needed, respectively.
摘要本工作的目的是确定估算小麦(Triticum aestivum)性状平均值所需的样本量,这些指标是通过测量、计数和称重获得的。采用随机收获的1790株植株进行了17次均匀性试验,评价了其以下性状:主茎和主茎穗长度(测量);叶、茎和穗的数量(计数);叶片、茎和穗的新鲜和干燥物质的质量(称重)。采用Bartlett检验、Kolmogorov-Smirnov检验和Welch方差分析。确定偏度、集中趋势和变异性,并计算样本量以估计13个被评估性状的平均值,考虑估计误差(95%置信区间的半幅值)等于平均值的5%、10%、15%和20%。按此顺序,通过称重、计数和测量来估计小麦性状的平均值的样本量减少。在95%置信区间内,以称重、计数和测量得到的小麦性状均值为最大误差为均值10%的试验中,每个处理分别需要117株、76株和9株。
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引用次数: 0
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