Pub Date : 2023-02-17DOI: 10.1590/s1678-3921.pab2023.v58.03025
J. C. R. Ribas, Anderson Lazzari, Lorena Beatriz Fagundes Gonzalez, Clandio Medeiros da Silva, L. Adamuchio, F. L. Cuquel, Rodrigo Sakurada, P. M. Pintro
Abstract The objective of this work was to evaluate the bromatological composition, bioactive compounds, antioxidant activities, and color of the leaves of olive cultivars Arbosana, Arbequina, Frantoio, Manzanilla, and Koroneiki in northwestern Paraná, Brazil. Leaves of each cultivar were collected for the experiment, which was carried out in a completely randomized design. The dry matter (DM) of olive leaves contains mostly carbohydrates (56.15 to 59.42 g 100 g-1 DM) and fibers (17.37 to 19.73 g 100 g-1 DM) in its bromatological composition. The total polyphenol content ranges from 13.27 to 22.81 mg GAE g-1, with cultivar Manzanilla standing out, and the flavonoid content from 6.50 to 7.65 mg QE g-1. Manzanilla shows the highest antioxidant activity of 93.56 and 78.15% in the DPPH and ABTS assays, respectively. When compared with the other cultivars, Manzanilla and Koroneiki have leaves with a higher green intensity and total chlorophyll content of 0.958 and 0.833 mg 100 mL 1, respectively. In the ABTS assay, there is a correlation both between total polyphenol content and antioxidant activity and between chlorophyll content and antioxidant activity. Olive leaves have characteristics that allow their application as an additive or ingredient for the development of food products with satisfactory antioxidant activity.
摘要本研究对巴西帕拉纳西北部橄榄品种Arbosana、Arbequina、Frantoio、Manzanilla和Koroneiki的色相组成、生物活性成分、抗氧化活性和叶片颜色进行了研究。试验收集各品种的叶片,采用完全随机设计。橄榄叶片干物质主要由碳水化合物(56.15 ~ 59.42 g 100 g-1 DM)和纤维(17.37 ~ 19.73 g 100 g-1 DM)组成。总多酚含量在13.27 ~ 22.81 mg QE g-1之间,其中以Manzanilla品种最为突出;总黄酮含量在6.50 ~ 7.65 mg QE g-1之间。在DPPH和ABTS试验中,Manzanilla的抗氧化活性最高,分别为93.56%和78.15%。与其他品种相比,Manzanilla和Koroneiki的叶片绿色强度更高,总叶绿素含量分别为0.958和0.833 mg 100 mL 1。在ABTS试验中,总多酚含量与抗氧化活性、叶绿素含量与抗氧化活性均存在相关性。橄榄叶的特性使其能够作为添加剂或成分用于开发具有令人满意的抗氧化活性的食品。
{"title":"Bioactive compounds and antioxidant activity of leaves from olive trees grown in Paraná, Brazil","authors":"J. C. R. Ribas, Anderson Lazzari, Lorena Beatriz Fagundes Gonzalez, Clandio Medeiros da Silva, L. Adamuchio, F. L. Cuquel, Rodrigo Sakurada, P. M. Pintro","doi":"10.1590/s1678-3921.pab2023.v58.03025","DOIUrl":"https://doi.org/10.1590/s1678-3921.pab2023.v58.03025","url":null,"abstract":"Abstract The objective of this work was to evaluate the bromatological composition, bioactive compounds, antioxidant activities, and color of the leaves of olive cultivars Arbosana, Arbequina, Frantoio, Manzanilla, and Koroneiki in northwestern Paraná, Brazil. Leaves of each cultivar were collected for the experiment, which was carried out in a completely randomized design. The dry matter (DM) of olive leaves contains mostly carbohydrates (56.15 to 59.42 g 100 g-1 DM) and fibers (17.37 to 19.73 g 100 g-1 DM) in its bromatological composition. The total polyphenol content ranges from 13.27 to 22.81 mg GAE g-1, with cultivar Manzanilla standing out, and the flavonoid content from 6.50 to 7.65 mg QE g-1. Manzanilla shows the highest antioxidant activity of 93.56 and 78.15% in the DPPH and ABTS assays, respectively. When compared with the other cultivars, Manzanilla and Koroneiki have leaves with a higher green intensity and total chlorophyll content of 0.958 and 0.833 mg 100 mL 1, respectively. In the ABTS assay, there is a correlation both between total polyphenol content and antioxidant activity and between chlorophyll content and antioxidant activity. Olive leaves have characteristics that allow their application as an additive or ingredient for the development of food products with satisfactory antioxidant activity.","PeriodicalId":19826,"journal":{"name":"Pesquisa Agropecuaria Brasileira","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67610128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-17DOI: 10.1590/s1678-3921.pab2023.v58.03006
Ana Cecilia Crenna, C. Oddino, D. F. Giordano, J. Giuggia, D. Giovanini
Abstract The objective of this work was to develop and validate a diagrammatic logarithmic scale to evaluate the damage caused by the Caliothrips phaseoli bean thrips to peanut (Arachis hypogaea). Leaflets of artificially infected peanut plants were collected, and the damaged leaf area (DLA) was calculated using the SisCob software. Six-, seven-, eight-, and nine-class scales were developed, and the range of each class was obtained with the 2-LOG software. The leaflets were analyzed by 12 raters using the four proposed scales. The scales were validated as to: precision and accuracy, using the linear regression between the DLA and estimated area; and reproducibility, by the linear regression between the estimations of the raters combined in pairs. The eight-class scale shows the best results in terms of accuracy and precision, as well as a high reproducibility.
{"title":"Diagrammatic scale to quantify the damage caused by bean thrips to the peanut crop","authors":"Ana Cecilia Crenna, C. Oddino, D. F. Giordano, J. Giuggia, D. Giovanini","doi":"10.1590/s1678-3921.pab2023.v58.03006","DOIUrl":"https://doi.org/10.1590/s1678-3921.pab2023.v58.03006","url":null,"abstract":"Abstract The objective of this work was to develop and validate a diagrammatic logarithmic scale to evaluate the damage caused by the Caliothrips phaseoli bean thrips to peanut (Arachis hypogaea). Leaflets of artificially infected peanut plants were collected, and the damaged leaf area (DLA) was calculated using the SisCob software. Six-, seven-, eight-, and nine-class scales were developed, and the range of each class was obtained with the 2-LOG software. The leaflets were analyzed by 12 raters using the four proposed scales. The scales were validated as to: precision and accuracy, using the linear regression between the DLA and estimated area; and reproducibility, by the linear regression between the estimations of the raters combined in pairs. The eight-class scale shows the best results in terms of accuracy and precision, as well as a high reproducibility.","PeriodicalId":19826,"journal":{"name":"Pesquisa Agropecuaria Brasileira","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67610053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-17DOI: 10.1590/s1678-3921.pab2023.v58.03008
Rafael Cavaca Alves Do Valle, Marcos Antônio da Silva, É. R. Alvarenga, Sylvia Veloso da Matta, E. M. Turra
Abstract The objective of this work was to evaluate the effect of different water salinity levels on the growth performance, survival, and masculinization rate of Nile tilapia (Oreochromis niloticus) larvae in a biofloc technology (BFT) system. Seven salinity levels (0, 2, 4, 6, 8, 10, and 12 g L-1) were tested during four weeks in the masculinization period after the absorption of the yolk sac in a matured biofloc system. The water quality variables were within the recommended range for Nile tilapia farming. However, the nitrite peaks were higher at higher salinity levels and were associated with the lower survival of fish at salinity levels equal to or higher than 6 g L-1. There was no difference between treatments for average final body weight and masculinization rate. Final biomass and survival decreased, and the feed offered as a proportion of final biomass showed the worst results because of the increase in salinity. Therefore, since higher masculinization rates are not obtained at slight and moderate levels of saline water, salinity should be kept close to 0 g L-1 for the masculinization protocol of Nile tilapia in a BFT, for a better survival and higher biomass of the fish, as well as a lower waste of the offered feed.
摘要本研究旨在研究生物絮团技术(BFT)系统中不同盐度对尼罗罗非鱼(Oreochromis niloticus)幼虫生长性能、存活率和雄性化率的影响。在成熟的生物团吸收卵黄囊后的雄性化期,在4周内测试了7种盐度水平(0、2、4、6、8、10和12 g L-1)。水质变量在尼罗罗非鱼养殖的推荐范围内。然而,在较高的盐度水平下,亚硝酸盐峰值较高,并且在等于或高于6 g L-1的盐度水平下,鱼类的存活率较低。不同处理之间的平均最终体重和雄性化率没有差异。最终生物量和存活率下降,饵料占最终生物量的比例由于盐度的增加而表现出最差的结果。因此,由于在轻度和中等水平的盐水中无法获得较高的雄性化率,因此在BFT中尼罗罗非鱼的雄性化方案中,盐度应保持在接近0 g L-1,以提高鱼的存活率和生物量,并降低所提供饲料的浪费。
{"title":"Water salinity during masculinization of Nile tilapia in biofloc system","authors":"Rafael Cavaca Alves Do Valle, Marcos Antônio da Silva, É. R. Alvarenga, Sylvia Veloso da Matta, E. M. Turra","doi":"10.1590/s1678-3921.pab2023.v58.03008","DOIUrl":"https://doi.org/10.1590/s1678-3921.pab2023.v58.03008","url":null,"abstract":"Abstract The objective of this work was to evaluate the effect of different water salinity levels on the growth performance, survival, and masculinization rate of Nile tilapia (Oreochromis niloticus) larvae in a biofloc technology (BFT) system. Seven salinity levels (0, 2, 4, 6, 8, 10, and 12 g L-1) were tested during four weeks in the masculinization period after the absorption of the yolk sac in a matured biofloc system. The water quality variables were within the recommended range for Nile tilapia farming. However, the nitrite peaks were higher at higher salinity levels and were associated with the lower survival of fish at salinity levels equal to or higher than 6 g L-1. There was no difference between treatments for average final body weight and masculinization rate. Final biomass and survival decreased, and the feed offered as a proportion of final biomass showed the worst results because of the increase in salinity. Therefore, since higher masculinization rates are not obtained at slight and moderate levels of saline water, salinity should be kept close to 0 g L-1 for the masculinization protocol of Nile tilapia in a BFT, for a better survival and higher biomass of the fish, as well as a lower waste of the offered feed.","PeriodicalId":19826,"journal":{"name":"Pesquisa Agropecuaria Brasileira","volume":"12 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67610061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-17DOI: 10.1590/s1678-3921.pab2023.v58.03050
Andreza Girelli, V. Sant'anna, M. Klein
Abstract The objective of this work was to develop a drying process using the foam-layer method to obtain the powder from butiá (Butia spp.) pulp, as well as to characterize this powder according to its yield and physicochemical and technological characteristics. The foam was optimized for density and stability by varying whipping times and albumin and xanthan gum concentrations. Foam drying was optimized for vitamin C retention and yield using different foam thicknesses and drying temperatures. The optimized foam showed a density of 0.25 g cm-3 and a high stability, being suitable for subsequent drying. The lowest foam thickness (0.50 cm) and the highest drying temperature (80°C) resulted in the highest retention of vitamin C, whereas the increase in drying temperature improved yield. The butiá pulp powder obtained under the optimized condition presented an acid pH of 3.25, a low humidity of 7.97%, a water activity of 0.206, a water retention capacity of 4.90 g H2O per gram of powder, a solubility of 74.40%, a soluble solids content of 61°Brix, and a predominantly yellow color. The foam-layer drying method can be used to obtain butiá pulp powder.
摘要:本研究的目的是开发一种泡沫层干燥法从丁铁 (Butia spp.)纸浆中获得粉末的工艺,并根据其产率、理化特性和工艺特性对该粉末进行表征。通过不同的搅拌时间、白蛋白和黄原胶浓度来优化泡沫的密度和稳定性。在不同的泡沫厚度和干燥温度下,优化了泡沫干燥对维生素C保留率和产率的影响。优化后的泡沫密度为0.25 g cm-3,稳定性高,适合后续干燥。在最低泡沫厚度(0.50 cm)和最高干燥温度(80℃)下,维生素C的保留率最高,干燥温度的升高可提高产量。在优化条件下制得的丁铁浆粉末酸pH值为3.25,低湿度为7.97%,水活度为0.206,保水容量为4.90 g H2O / g,溶解度为74.40%,可溶性固形物含量为61°Brix,颜色以黄色为主。泡沫层干燥法可获得浆粉。
{"title":"Drying of butiá pulp by the foam-layer method and characterization of the obtained powder","authors":"Andreza Girelli, V. Sant'anna, M. Klein","doi":"10.1590/s1678-3921.pab2023.v58.03050","DOIUrl":"https://doi.org/10.1590/s1678-3921.pab2023.v58.03050","url":null,"abstract":"Abstract The objective of this work was to develop a drying process using the foam-layer method to obtain the powder from butiá (Butia spp.) pulp, as well as to characterize this powder according to its yield and physicochemical and technological characteristics. The foam was optimized for density and stability by varying whipping times and albumin and xanthan gum concentrations. Foam drying was optimized for vitamin C retention and yield using different foam thicknesses and drying temperatures. The optimized foam showed a density of 0.25 g cm-3 and a high stability, being suitable for subsequent drying. The lowest foam thickness (0.50 cm) and the highest drying temperature (80°C) resulted in the highest retention of vitamin C, whereas the increase in drying temperature improved yield. The butiá pulp powder obtained under the optimized condition presented an acid pH of 3.25, a low humidity of 7.97%, a water activity of 0.206, a water retention capacity of 4.90 g H2O per gram of powder, a solubility of 74.40%, a soluble solids content of 61°Brix, and a predominantly yellow color. The foam-layer drying method can be used to obtain butiá pulp powder.","PeriodicalId":19826,"journal":{"name":"Pesquisa Agropecuaria Brasileira","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67609813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1590/s1678-3921.pab2023.v58.03187
Antonioni Campos Acácio Moliterno, Maycon Roberto da Silva, Maria Aparecida Cassilha Zawadneak, Paulo Henrique Gorgatti Zarbin
Abstract The objective of this work was to describe the sexual dimorphism and mating behavior of Lobiopa insularis for the development of pest control and management programs. The morphological characteristics for separating males and females were described with the aid of a stereomicroscope. Mating sequence was recorded over 72 hours with a digital camera. There are differences between males and females in the last abdomen segments, and males maintain a guarding position after mating. The obtained data can be used in monitoring programs to precisely identify the sex of the insects, also shedding light on the ecological features of this pest and Nitidulidae.
{"title":"Mating behavior and sexual dimorphism of the Lobiopa insularis (Coleoptera: Nitidulidae) strawberry pest","authors":"Antonioni Campos Acácio Moliterno, Maycon Roberto da Silva, Maria Aparecida Cassilha Zawadneak, Paulo Henrique Gorgatti Zarbin","doi":"10.1590/s1678-3921.pab2023.v58.03187","DOIUrl":"https://doi.org/10.1590/s1678-3921.pab2023.v58.03187","url":null,"abstract":"Abstract The objective of this work was to describe the sexual dimorphism and mating behavior of Lobiopa insularis for the development of pest control and management programs. The morphological characteristics for separating males and females were described with the aid of a stereomicroscope. Mating sequence was recorded over 72 hours with a digital camera. There are differences between males and females in the last abdomen segments, and males maintain a guarding position after mating. The obtained data can be used in monitoring programs to precisely identify the sex of the insects, also shedding light on the ecological features of this pest and Nitidulidae.","PeriodicalId":19826,"journal":{"name":"Pesquisa Agropecuaria Brasileira","volume":"105 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135059734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1590/s1678-3921.pab2023.v58.03252
Paulo Henrique Karling Facchinello, Ivan Ricardo Carvalho, Eduardo Anibele Streck, Gabriel Almeida Aguiar, Janaína Goveia, Michele Feijó, Roberto Ramos Pereira, Paulo Ricardo Reis Fagundes, Murilo Vieira Loro, Luciano Carlos da Maia, Ariano Martins de Magalhães Júnior
Abstract The objective of this work was to determine the genetic correlations and the direct and indirect associations of agronomic traits and grain quality attributes with the percentage of whole grains in flood irrigated rice. The experiment was carried out in two environments, in a randomized complete block design with three replicates, using 23 irrigated rice genotypes. The evaluated traits were: percentage of whole grains, caryopsis length, caryopsis width, panicle length, panicle weight, 1,000 grain weight, days to flowering, percentage of chalky grains with white belly, total chalky area, total whiteness, vitreous whiteness, and defects in coloring. The percentage of grains with white belly and total chalky area were positively correlated, whereas the percentage of grains with white belly and vitreous whiteness were negatively correlated. The traits caryopsis width, percentage of chalky grains with white belly, panicle weight, and 1,000 grain weight showed indirect effects on whole-grain yield response according to total chalky area and total whiteness. Total chalky area and total whiteness are the factors that most negatively influence the percentage of whole grains according to the genotypic correlations and direct effects.
{"title":"Genetic cause and effect interrelationships for grain quality attributes of irrigated rice","authors":"Paulo Henrique Karling Facchinello, Ivan Ricardo Carvalho, Eduardo Anibele Streck, Gabriel Almeida Aguiar, Janaína Goveia, Michele Feijó, Roberto Ramos Pereira, Paulo Ricardo Reis Fagundes, Murilo Vieira Loro, Luciano Carlos da Maia, Ariano Martins de Magalhães Júnior","doi":"10.1590/s1678-3921.pab2023.v58.03252","DOIUrl":"https://doi.org/10.1590/s1678-3921.pab2023.v58.03252","url":null,"abstract":"Abstract The objective of this work was to determine the genetic correlations and the direct and indirect associations of agronomic traits and grain quality attributes with the percentage of whole grains in flood irrigated rice. The experiment was carried out in two environments, in a randomized complete block design with three replicates, using 23 irrigated rice genotypes. The evaluated traits were: percentage of whole grains, caryopsis length, caryopsis width, panicle length, panicle weight, 1,000 grain weight, days to flowering, percentage of chalky grains with white belly, total chalky area, total whiteness, vitreous whiteness, and defects in coloring. The percentage of grains with white belly and total chalky area were positively correlated, whereas the percentage of grains with white belly and vitreous whiteness were negatively correlated. The traits caryopsis width, percentage of chalky grains with white belly, panicle weight, and 1,000 grain weight showed indirect effects on whole-grain yield response according to total chalky area and total whiteness. Total chalky area and total whiteness are the factors that most negatively influence the percentage of whole grains according to the genotypic correlations and direct effects.","PeriodicalId":19826,"journal":{"name":"Pesquisa Agropecuaria Brasileira","volume":"129 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135060328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1590/s1678-3921.pab2023.v58.03140
Luiz Francisco Machado Pfeifer, Jair Sábio de Oliveira Júnior, Marcelo Marcondes Seneda, Higor Souza de Camargo, Fábio Morotti, Evelyn Rabelo Andrade, Elizângela Mirian Moreira, Jéssica Souza Andrade, George Moreira da Silva, Marlos Oliveira Porto, Jucilene Cavali
Abstract The objective of this work was to investigate whether puberty and parturition alter the antral follicle count (AFC) in female Nelore (Bos indicus) beef cattle. Two experiments were carried out to compare AFC between the prepubertal and pubertal periods and between the prepubertal and postpartum periods. AFC and follicle concentrations did not differ between the prepubertal and pubertal periods. However, the ovarian area increased after puberty. In addition, AFC and the ovarian area were greater in the postpartum than in the prepubertal period. Therefore, AFC is not affected by the puberty of Nelore heifers, but increases significantly when the prepubertal heifers become cows.
{"title":"Puberty and parturition on the antral follicle count of female Nelore beef cattle","authors":"Luiz Francisco Machado Pfeifer, Jair Sábio de Oliveira Júnior, Marcelo Marcondes Seneda, Higor Souza de Camargo, Fábio Morotti, Evelyn Rabelo Andrade, Elizângela Mirian Moreira, Jéssica Souza Andrade, George Moreira da Silva, Marlos Oliveira Porto, Jucilene Cavali","doi":"10.1590/s1678-3921.pab2023.v58.03140","DOIUrl":"https://doi.org/10.1590/s1678-3921.pab2023.v58.03140","url":null,"abstract":"Abstract The objective of this work was to investigate whether puberty and parturition alter the antral follicle count (AFC) in female Nelore (Bos indicus) beef cattle. Two experiments were carried out to compare AFC between the prepubertal and pubertal periods and between the prepubertal and postpartum periods. AFC and follicle concentrations did not differ between the prepubertal and pubertal periods. However, the ovarian area increased after puberty. In addition, AFC and the ovarian area were greater in the postpartum than in the prepubertal period. Therefore, AFC is not affected by the puberty of Nelore heifers, but increases significantly when the prepubertal heifers become cows.","PeriodicalId":19826,"journal":{"name":"Pesquisa Agropecuaria Brasileira","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135700719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1590/s1678-3921.pab2023.v58.03167
Dhiôvanna Corrêia Rocha, Thaís Ignez da Cruz, João Augusto Vieira de Oliveira, Isabela Pavanelli de Souza, Beata Dedicova, Alexandre Siqueira Guedes Coelho, Rosana Pereira Vianello, Claudio Brondani
Abstract The objective of this work was to evaluate the physiological, phenotypic, and gene expression parameters in genetically modified (GM) rice plants that overexpress the Oryza sativa Vacuolar H+-Pyrophosphatase 1 (OVP1) gene, compared with non-genetically modified (NGM) rice. GM and NGM plants of the BRSMG Curinga cultivar were evaluated in two experiments, in a laboratory and greenhouse, in a randomized complete block design, with four replicates. Agronomic traits of interest were estimated, and transcriptome analysis and gene expression quantification were carried out. GM plants showed a 31 and 21% higher number of spikelets per panicle and total number of grains per panicle, respectively, in comparison with NGM plants. Physiological changes occurred during the grain-filling stage, in which GM plants presented a photosynthetic rate and carboxylation efficiency 61 and 89% higher than those of NGM plants, respectively. The overexpression of the OVP1 gene favors the upregulation of some photosynthesis genes and the increase in the number of spikelets and in the photosynthetic rate, but does not favor the increase in grain yield.
{"title":"Physiological and phenotypical effects of the overexpression of the OVP1 gene in Japonica rice","authors":"Dhiôvanna Corrêia Rocha, Thaís Ignez da Cruz, João Augusto Vieira de Oliveira, Isabela Pavanelli de Souza, Beata Dedicova, Alexandre Siqueira Guedes Coelho, Rosana Pereira Vianello, Claudio Brondani","doi":"10.1590/s1678-3921.pab2023.v58.03167","DOIUrl":"https://doi.org/10.1590/s1678-3921.pab2023.v58.03167","url":null,"abstract":"Abstract The objective of this work was to evaluate the physiological, phenotypic, and gene expression parameters in genetically modified (GM) rice plants that overexpress the Oryza sativa Vacuolar H+-Pyrophosphatase 1 (OVP1) gene, compared with non-genetically modified (NGM) rice. GM and NGM plants of the BRSMG Curinga cultivar were evaluated in two experiments, in a laboratory and greenhouse, in a randomized complete block design, with four replicates. Agronomic traits of interest were estimated, and transcriptome analysis and gene expression quantification were carried out. GM plants showed a 31 and 21% higher number of spikelets per panicle and total number of grains per panicle, respectively, in comparison with NGM plants. Physiological changes occurred during the grain-filling stage, in which GM plants presented a photosynthetic rate and carboxylation efficiency 61 and 89% higher than those of NGM plants, respectively. The overexpression of the OVP1 gene favors the upregulation of some photosynthesis genes and the increase in the number of spikelets and in the photosynthetic rate, but does not favor the increase in grain yield.","PeriodicalId":19826,"journal":{"name":"Pesquisa Agropecuaria Brasileira","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136302687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1590/s1678-3921.pab2023.v58.03203
Victoria Freitas de Oliveira, Eduardo Venske, Cássia Fernanda Stafen, Fernanda Pollo Paniz, Tatiana Pedron, Rodrigo Mendes Pereira, Ariano Martins de Magalhães Júnior, Luciano Carlos da Maia, Antonio Costa de Oliveira, Bruno Lemos Batista, Camila Pegoraro
Abstract The objective of this work was to map the chromosomal regions responsible for iron accumulation in rice grains, in Southern Brazil. Eighty-one rice accessions were genotyped and phenotyped for Fe accumulation. Single nucleotide polymorphisms were mapped in the whole grain on chromosomes 1, 5, 6, and 10, from which 13 candidate genes were identified. Some of the genes, such as O s10g040680 0, seem to have a relationship with Fe homeostasis, while others are related to other metabolic processes or have an unknown function.
{"title":"Genome-wide association of iron content in rice grains grown in Southern Brazil","authors":"Victoria Freitas de Oliveira, Eduardo Venske, Cássia Fernanda Stafen, Fernanda Pollo Paniz, Tatiana Pedron, Rodrigo Mendes Pereira, Ariano Martins de Magalhães Júnior, Luciano Carlos da Maia, Antonio Costa de Oliveira, Bruno Lemos Batista, Camila Pegoraro","doi":"10.1590/s1678-3921.pab2023.v58.03203","DOIUrl":"https://doi.org/10.1590/s1678-3921.pab2023.v58.03203","url":null,"abstract":"Abstract The objective of this work was to map the chromosomal regions responsible for iron accumulation in rice grains, in Southern Brazil. Eighty-one rice accessions were genotyped and phenotyped for Fe accumulation. Single nucleotide polymorphisms were mapped in the whole grain on chromosomes 1, 5, 6, and 10, from which 13 candidate genes were identified. Some of the genes, such as O s10g040680 0, seem to have a relationship with Fe homeostasis, while others are related to other metabolic processes or have an unknown function.","PeriodicalId":19826,"journal":{"name":"Pesquisa Agropecuaria Brasileira","volume":"2012 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136305106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1590/s1678-3921.pab2023.v58.03246
Letícia Lopes de Godoi, Laya Kannan Silva Alves, Ana Luísa Neves Alvarenga Dias, Hebert Silveira, Cesar Augusto Pospissil Garbossa
Abstract The objective of this work was to evaluate the replacement of choline chloride supplementation by herbal choline in the diet of nursery piglets. The experimental design was randomized complete blocks (initial weight and sex) with 80 piglets, in five treatments, with eight replicates. The treatments consisted of: negative control, basal diet supplemented with 300 mg kg-1 choline via choline chloride, basal diet supplemented with 600 mg kg-1 choline via choline chloride, basal diet supplemented with 100 mg kg-1 herbal choline, and basal diet supplemented with 200 mg kg-1 herbal choline. Zootechnical performance data, blood parameters, and economic viability were analyzed. Herbal choline supplementation increases the body weight and daily feed intake of nursery piglets. The supplementation with 100 mg kg-1 herbal choline presents the highest return on investment. Herbal choline can be used as a source of choline supplementation in the diet of nursery piglets to replace choline chloride.
{"title":"Herbal choline as an alternative to choline chloride in the diet of nursery piglets","authors":"Letícia Lopes de Godoi, Laya Kannan Silva Alves, Ana Luísa Neves Alvarenga Dias, Hebert Silveira, Cesar Augusto Pospissil Garbossa","doi":"10.1590/s1678-3921.pab2023.v58.03246","DOIUrl":"https://doi.org/10.1590/s1678-3921.pab2023.v58.03246","url":null,"abstract":"Abstract The objective of this work was to evaluate the replacement of choline chloride supplementation by herbal choline in the diet of nursery piglets. The experimental design was randomized complete blocks (initial weight and sex) with 80 piglets, in five treatments, with eight replicates. The treatments consisted of: negative control, basal diet supplemented with 300 mg kg-1 choline via choline chloride, basal diet supplemented with 600 mg kg-1 choline via choline chloride, basal diet supplemented with 100 mg kg-1 herbal choline, and basal diet supplemented with 200 mg kg-1 herbal choline. Zootechnical performance data, blood parameters, and economic viability were analyzed. Herbal choline supplementation increases the body weight and daily feed intake of nursery piglets. The supplementation with 100 mg kg-1 herbal choline presents the highest return on investment. Herbal choline can be used as a source of choline supplementation in the diet of nursery piglets to replace choline chloride.","PeriodicalId":19826,"journal":{"name":"Pesquisa Agropecuaria Brasileira","volume":"57 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136305102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}