Pub Date : 2018-01-01DOI: 10.4172/2153-2435.1000584
Al-Okab Ra, Galil Msa, Ahmed N. Al‐Hakimi
Green analytical chemistry is considered as a branch of the green chemistry and the main its goal is to achieve more green analysis in quality control laboratories through different direction, such as replacing or minimizing toxic reagents and modify or replace analytical techniques or methods with safer ones. We develop a simple, accurate and sensitive spectrophotometric procedure for determination of Sulfamethoxazole (SMX) using phenoxazine (PNZ) as green analytical reagent. The method is based on oxidation in aqueous mildly acidic medium primary amine group of SMX and coupling with PNZ in the prescient iron (III) to form a stable color having maximum absorption at 520 nm. Beer's law was obeyed in the range concentration of 0.1-6 mg/l and molar absorptivity 6.105 × 104 L.mol-1.cm-1. Sandell’s sensitivity 0.003 μg.cm-2 and detection limit (DL) 0.021 ppm. The direct determination of SMX in pure form and in its pharmaceutical formulations method successfully applied with very good recoveries 98.70%-101.5%. Green spectrophotometric analytical analyses become unique when the pharmaceutical drugs reagents combine with low concentration to determinate or estimate pharmaceutical drugs.
{"title":"Development Green Spectrophotometric Method for Determination of Sulfamethoxazole in Pure and Pharmaceutical Formulations","authors":"Al-Okab Ra, Galil Msa, Ahmed N. Al‐Hakimi","doi":"10.4172/2153-2435.1000584","DOIUrl":"https://doi.org/10.4172/2153-2435.1000584","url":null,"abstract":"Green analytical chemistry is considered as a branch of the green chemistry and the main its goal is to achieve more green analysis in quality control laboratories through different direction, such as replacing or minimizing toxic reagents and modify or replace analytical techniques or methods with safer ones. We develop a simple, accurate and sensitive spectrophotometric procedure for determination of Sulfamethoxazole (SMX) using phenoxazine (PNZ) as green analytical reagent. The method is based on oxidation in aqueous mildly acidic medium primary amine group of SMX and coupling with PNZ in the prescient iron (III) to form a stable color having maximum absorption at 520 nm. Beer's law was obeyed in the range concentration of 0.1-6 mg/l and molar absorptivity 6.105 × 104 L.mol-1.cm-1. Sandell’s sensitivity 0.003 μg.cm-2 and detection limit (DL) 0.021 ppm. The direct determination of SMX in pure form and in its pharmaceutical formulations method successfully applied with very good recoveries 98.70%-101.5%. Green spectrophotometric analytical analyses become unique when the pharmaceutical drugs reagents combine with low concentration to determinate or estimate pharmaceutical drugs.","PeriodicalId":19833,"journal":{"name":"Pharmaceutica Analytica Acta","volume":"77 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89830295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.4172/2153-2435.1000599
Jain Ps, Ansari Na, Surana Sj
The aim of this work is to establish developed and validated method for the pharmaceutical analysis of Felodipine in bulk and pharmaceutical formulation by High Performance Thin Layer Chromatography HPTLC (NP) and Reverse Phase-High Performance Thin Layer Chromatography RP-HPTLC (RP). Chromatographic separation was performed on Pre-coated aluminum plates with 250 μm layer of Silica gel 60 F254 and Silica gel 60 RP-18 TLC F254S using Toluene: Methanol (8:2 v/v) and acetonitrile: water: glacial acetic acid (8:2:1 v/v/v) as a mobile phase, respectively. Scanning was carried out densitometrically at 237 nm. The Rf value of Felodipine in NP and RP were 0.40 and 0.53 and the reliability of the method was assessed by the evaluation of linearity which was found to be 300-1800 and 500-3000 ng/band with the r2 =0.998 correlation coefficient along with the accuracy of the method in terms of % recovery was found to be from 98-101 ± 1.04 % and 99-100 ± 0.47 % and the limit of detection and quantification were 11.51, 34.90 and 29.90, 90.61, respectively. The method can be used for routine analysis of Felodipine in bulk and pharmaceutical formulation.
{"title":"HPTLC and RP-HPTLC Method Development and Validation for the Estimation of Felodipine in Bulk and Pharmaceutical Formulation","authors":"Jain Ps, Ansari Na, Surana Sj","doi":"10.4172/2153-2435.1000599","DOIUrl":"https://doi.org/10.4172/2153-2435.1000599","url":null,"abstract":"The aim of this work is to establish developed and validated method for the pharmaceutical analysis of Felodipine in bulk and pharmaceutical formulation by High Performance Thin Layer Chromatography HPTLC (NP) and Reverse Phase-High Performance Thin Layer Chromatography RP-HPTLC (RP). Chromatographic separation was performed on Pre-coated aluminum plates with 250 μm layer of Silica gel 60 F254 and Silica gel 60 RP-18 TLC F254S using Toluene: Methanol (8:2 v/v) and acetonitrile: water: glacial acetic acid (8:2:1 v/v/v) as a mobile phase, respectively. Scanning was carried out densitometrically at 237 nm. The Rf value of Felodipine in NP and RP were 0.40 and 0.53 and the reliability of the method was assessed by the evaluation of linearity which was found to be 300-1800 and 500-3000 ng/band with the r2 =0.998 correlation coefficient along with the accuracy of the method in terms of % recovery was found to be from 98-101 ± 1.04 % and 99-100 ± 0.47 % and the limit of detection and quantification were 11.51, 34.90 and 29.90, 90.61, respectively. The method can be used for routine analysis of Felodipine in bulk and pharmaceutical formulation.","PeriodicalId":19833,"journal":{"name":"Pharmaceutica Analytica Acta","volume":"26 5","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91497794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.4172/2153-2435.1000597
R. El-Gamal, F. Belal
A highly sensitive, simple and rapid first derivative synchronous spectrofluorimetric method was utilized for the determination of daclatasvir dihydrochloride (DCV) in presence of its oxidative and photolytic degradation products. Where synchronous 1st derivative spectrofluorimetric approach was utilized to quantitatively determine DCV at 373 nm in presence of its oxidative degradation product and at 388 nm in presence of its photolytic degradation product that is obtained by exposing DCV to UV light at 312 nm, these were the zero-crossing wavelengths of degradation products without interference. The synchronous fluorescence was scanned at Δ λ of 80 nm. The method was found to be linear across the concentration range of 0.5-5.0 ng/mL with lower detection limit of 0.090 and lower quantification limit of 0.275 ng/mL (at 373 nm) and 0.268 ng/mL (at 388 nm). The adopted approach was successfully applied to commercial tablet and the results exhibited that the derivative synchronous fluorescence spectroscopy is a stabilityindicating method, suitable for routine use within a short analysis time. The proposed method was carefully validated for linearity, accuracy, precision, specificity and robustness.
{"title":"Stability Indicating 1st Derivative Synchronous Spectrofluorimetric Method for the Determination of the Newly Approved Antiviral Drug Daclatasvir in Presence of Its Oxidative and Photolytic Degradation Products: Application to Tablet Dosage Form","authors":"R. El-Gamal, F. Belal","doi":"10.4172/2153-2435.1000597","DOIUrl":"https://doi.org/10.4172/2153-2435.1000597","url":null,"abstract":"A highly sensitive, simple and rapid first derivative synchronous spectrofluorimetric method was utilized for the determination of daclatasvir dihydrochloride (DCV) in presence of its oxidative and photolytic degradation products. Where synchronous 1st derivative spectrofluorimetric approach was utilized to quantitatively determine DCV at 373 nm in presence of its oxidative degradation product and at 388 nm in presence of its photolytic degradation product that is obtained by exposing DCV to UV light at 312 nm, these were the zero-crossing wavelengths of degradation products without interference. The synchronous fluorescence was scanned at Δ λ of 80 nm. The method was found to be linear across the concentration range of 0.5-5.0 ng/mL with lower detection limit of 0.090 and lower quantification limit of 0.275 ng/mL (at 373 nm) and 0.268 ng/mL (at 388 nm). The adopted approach was successfully applied to commercial tablet and the results exhibited that the derivative synchronous fluorescence spectroscopy is a stabilityindicating method, suitable for routine use within a short analysis time. The proposed method was carefully validated for linearity, accuracy, precision, specificity and robustness.","PeriodicalId":19833,"journal":{"name":"Pharmaceutica Analytica Acta","volume":"29 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84428780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.4172/2153-2435.1000582
T. Ac, M. Viswanathan, K. Balakrishna, A. Patra
ObjectiveIn the present study, the plant Chamaecrista nigricans (Siruavuri in Tamil) was selected to isolate, elucidate and identify the chemical constituents present in it.MethodsLeaves were collected, shade-dried, coarsely powdered using a pulvarizor, successively extracted with various solvents of increasing polarity such as hexane, chloroform and methanol using Soxhlet apparatus. Methanol leaf extract was used for isolation and identification of chemical constituents. Column chromatography (CC) and thin layer chromatography (TLC) were used for separation and purification of chemical constituents while the isolated pure compounds were identified using UV-VIS, IR, 1H and 13C NMR spectra. GC-MS analysis was carried out to identify the chemical constituents.ResultsThree anthraquinones such as emodin, chrysophanol and physcion were isolated and identified. GC-MS analysis helped to identify diisooctyl ester 1,2-benzenedicarboxylic acid, methyl ester, (Z, Z, Z)-9,12,15-octadecatrienoic acid, nitric acid nonyl ester, 4-C-methyl-myo-inositol, n-hexadecanoic acid, 2-methyl-butanoic acid, and, octadecanoic acid.ConclusionMedicinally valuable bioactive natural compounds in this plant proved its importance in drug industry for drug development against various diseases.
目的对泰米尔植物Chamaecrista nigricans (Siruavuri)进行分离、分析和鉴定。方法采集叶片，遮荫干燥，用粉剂粗制成粉，用索氏仪分别用正己烷、氯仿、甲醇等极性逐渐增大的溶剂提取。采用甲醇叶提取物对其化学成分进行分离鉴定。采用柱层析(CC)和薄层析(TLC)对化学成分进行分离纯化，并利用UV-VIS、IR、1H和13C NMR对分离得到的纯化合物进行鉴定。采用气相色谱-质谱分析鉴定其化学成分。结果分离鉴定出了大黄素、大黄酚和物理三种蒽醌类化合物。GC-MS分析鉴定了1,2-苯二羧酸二异辛基酯、甲酯、(Z, Z, Z)-9,12,15-十八碳三烯酸、硝酸壬基酯、4- c -甲基肌醇、正十六烷酸、2-甲基丁酸和十八烷酸。结论该植物中含有具有药用价值的天然活性化合物，对开发抗多种疾病的药物具有重要意义。
{"title":"Phytochemical Analysis in the Leaves of Chamaecrista nigricans (Leguminosae)","authors":"T. Ac, M. Viswanathan, K. Balakrishna, A. Patra","doi":"10.4172/2153-2435.1000582","DOIUrl":"https://doi.org/10.4172/2153-2435.1000582","url":null,"abstract":"ObjectiveIn the present study, the plant Chamaecrista nigricans (Siruavuri in Tamil) was selected to isolate, elucidate and identify the chemical constituents present in it.MethodsLeaves were collected, shade-dried, coarsely powdered using a pulvarizor, successively extracted with various solvents of increasing polarity such as hexane, chloroform and methanol using Soxhlet apparatus. Methanol leaf extract was used for isolation and identification of chemical constituents. Column chromatography (CC) and thin layer chromatography (TLC) were used for separation and purification of chemical constituents while the isolated pure compounds were identified using UV-VIS, IR, 1H and 13C NMR spectra. GC-MS analysis was carried out to identify the chemical constituents.ResultsThree anthraquinones such as emodin, chrysophanol and physcion were isolated and identified. GC-MS analysis helped to identify diisooctyl ester 1,2-benzenedicarboxylic acid, methyl ester, (Z, Z, Z)-9,12,15-octadecatrienoic acid, nitric acid nonyl ester, 4-C-methyl-myo-inositol, n-hexadecanoic acid, 2-methyl-butanoic acid, and, octadecanoic acid.ConclusionMedicinally valuable bioactive natural compounds in this plant proved its importance in drug industry for drug development against various diseases.","PeriodicalId":19833,"journal":{"name":"Pharmaceutica Analytica Acta","volume":"41 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76297492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.4172/2153-2435.1000579
Raveendra Bg, KumarRA, Shaheen Sd, A. Greeshma, M. Satyanarayana, Manikanta Rsht, Syam Cpb
A new approach developed for stability-indicating, simultaneous estimation of Saxagliptin and Dapagliflozin in rat serum by using UV spectroscopy. Saxagliptin detection wave length was at 222 nm with water is solvent and Dapagliflozin detection wave length was at 274 nm with phosphate buffer pH 6.8 is solvent. Both drugs are obeyed the beers-lamberts concentration range was founds to be 1-10 μg/mL. The present method was optimized and validated in spiked rat serum according to ICH guidelines. All validation parameters were found to be within the acceptable limits and stability-indicating studies were conducted under different conditions founds in negligible. The present method was simple and sensitive; it was successfully adopted for the simultaneous estimation of Saxagliptin and Dapagliflozin in rat serum samples by using UV spectroscopy.
{"title":"A Novel Stability-Indicating Method for the Simultaneous Estimation of Saxagliptin and Dapagliflozin in Rat Serum by Using UV Spectroscopy","authors":"Raveendra Bg, KumarRA, Shaheen Sd, A. Greeshma, M. Satyanarayana, Manikanta Rsht, Syam Cpb","doi":"10.4172/2153-2435.1000579","DOIUrl":"https://doi.org/10.4172/2153-2435.1000579","url":null,"abstract":"A new approach developed for stability-indicating, simultaneous estimation of Saxagliptin and Dapagliflozin in rat serum by using UV spectroscopy. Saxagliptin detection wave length was at 222 nm with water is solvent and Dapagliflozin detection wave length was at 274 nm with phosphate buffer pH 6.8 is solvent. Both drugs are obeyed the beers-lamberts concentration range was founds to be 1-10 μg/mL. The present method was optimized and validated in spiked rat serum according to ICH guidelines. All validation parameters were found to be within the acceptable limits and stability-indicating studies were conducted under different conditions founds in negligible. The present method was simple and sensitive; it was successfully adopted for the simultaneous estimation of Saxagliptin and Dapagliflozin in rat serum samples by using UV spectroscopy.","PeriodicalId":19833,"journal":{"name":"Pharmaceutica Analytica Acta","volume":"88 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81418100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.4172/2153-2435.1000588
Y. Wei, Bachu Rd, Y. Sari, Boddu Shs
MS-153 is a novel pyrazoline compound that serves as a potential neuroprotective therapeutic agent during ischemia. Development of a convenient, quick, and robust analytical method to quantify MS-153 in biological samples is necessary to understand it’s in vivo pharmacokinetic/pharmacodynamics (PKPD) profiles. An isocratic reverse-phase HPLC method was developed and validated for quantification of MS-153. Chromatographic separation was achieved with a C18 column. Mobile phase consisting of water/acetonitrile (85/15, v/v) was pumped at a flow rate of 1.0 mL/min. The retention time of MS-153 (λmax=260 nm) was found to be 7.15 minutes. A calibration curve established over a range of 0.78125 ng to 500 ng showed a correlation coefficient of 1.0. The LOD and LOQ were found to be 0.164 and 0.496 ng, respectively. The accuracy, intra-day precision, and inter-day precision were found to be 99.97% to 101.66% (recovery), 0.21% to 0.55% (RSD), and 0.32% to 0.82% (RSD), respectively. MS-153 was analysed from biological samples by adding methanol to remove proteins in the biological matrix prior to HPLC analysis. The extraction efficiency was found to be 100%. The developed method was also used to analyse the stability of MS-153 in diluted blank rat plasma and brain homogenate samples. Results indicated that no significant degradation of MS-153 was observed at 37°C for 6 h.
{"title":"Development and Validation of a HPLC Method for MS-153 Quantification: Assessment of its Stability in Rat Plasma and Brain Homogenate","authors":"Y. Wei, Bachu Rd, Y. Sari, Boddu Shs","doi":"10.4172/2153-2435.1000588","DOIUrl":"https://doi.org/10.4172/2153-2435.1000588","url":null,"abstract":"MS-153 is a novel pyrazoline compound that serves as a potential neuroprotective therapeutic agent during ischemia. Development of a convenient, quick, and robust analytical method to quantify MS-153 in biological samples is necessary to understand it’s in vivo pharmacokinetic/pharmacodynamics (PKPD) profiles. An isocratic reverse-phase HPLC method was developed and validated for quantification of MS-153. Chromatographic separation was achieved with a C18 column. Mobile phase consisting of water/acetonitrile (85/15, v/v) was pumped at a flow rate of 1.0 mL/min. The retention time of MS-153 (λmax=260 nm) was found to be 7.15 minutes. A calibration curve established over a range of 0.78125 ng to 500 ng showed a correlation coefficient of 1.0. The LOD and LOQ were found to be 0.164 and 0.496 ng, respectively. The accuracy, intra-day precision, and inter-day precision were found to be 99.97% to 101.66% (recovery), 0.21% to 0.55% (RSD), and 0.32% to 0.82% (RSD), respectively. MS-153 was analysed from biological samples by adding methanol to remove proteins in the biological matrix prior to HPLC analysis. The extraction efficiency was found to be 100%. The developed method was also used to analyse the stability of MS-153 in diluted blank rat plasma and brain homogenate samples. Results indicated that no significant degradation of MS-153 was observed at 37°C for 6 h.","PeriodicalId":19833,"journal":{"name":"Pharmaceutica Analytica Acta","volume":"16 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82128999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.4172/2153-2435-C4-041
pLisa Elvirip
{"title":"Understanding the importance of diversity of particle size methods based on laser diffraction","authors":"pLisa Elvirip","doi":"10.4172/2153-2435-C4-041","DOIUrl":"https://doi.org/10.4172/2153-2435-C4-041","url":null,"abstract":"","PeriodicalId":19833,"journal":{"name":"Pharmaceutica Analytica Acta","volume":"386 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78313107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.4172/2153-2435.1000591
Mhaske Db, Sreedharan S, Mahadik Kr
Bioenhancers can be defined as chemical entities, which when mixed with drugs promote and augment their bioavailability without showing any synergistic effect with the drug. The factors like toxicity, cost, poor bioavailability and long term administration of drugs give rise to the need of bioenhancers which help overcome most of these problems. Piper species produce a pungent alkaloid named Piperine or 1-peperoyl piperidine. Piperine increases permeability at the site of absorption by modulating lipid environment and membrane dynamics. Piperine has a molecular structure that is suitable for enzyme inhibition. It augments the bioavailability of several drugs like carbamazepine, curcumin, ciprofloxacin, ampicillin, metronidazole, oxytetracycline and many others by inhibiting various metabolizing enzymes. Thus piperine, being an efficacious inhibitor of drug metabolism is a powerful enhancer of absorption. The following review explores the mechanism, metabolism inhibition, influence of structural changes on activity, and drugs bioenhanced by piperine. It provides an insight on the application of piperine as an effective bioenhancer and the superiority of a bioenhanced drug formulation over the one without a bioenhancer. This concept which is found to be beneficial, has its roots in Ayurveda-the traditional Indian system of medicine and has been applied to various drugs. It presents a fine instance of the advantage of amalgamating a traditional system with contemporary medicine.
{"title":"Role of Piperine as an Effective Bioenhancer in Drug Absorption","authors":"Mhaske Db, Sreedharan S, Mahadik Kr","doi":"10.4172/2153-2435.1000591","DOIUrl":"https://doi.org/10.4172/2153-2435.1000591","url":null,"abstract":"Bioenhancers can be defined as chemical entities, which when mixed with drugs promote and augment their bioavailability without showing any synergistic effect with the drug. The factors like toxicity, cost, poor bioavailability and long term administration of drugs give rise to the need of bioenhancers which help overcome most of these problems. Piper species produce a pungent alkaloid named Piperine or 1-peperoyl piperidine. Piperine increases permeability at the site of absorption by modulating lipid environment and membrane dynamics. Piperine has a molecular structure that is suitable for enzyme inhibition. It augments the bioavailability of several drugs like carbamazepine, curcumin, ciprofloxacin, ampicillin, metronidazole, oxytetracycline and many others by inhibiting various metabolizing enzymes. Thus piperine, being an efficacious inhibitor of drug metabolism is a powerful enhancer of absorption. The following review explores the mechanism, metabolism inhibition, influence of structural changes on activity, and drugs bioenhanced by piperine. It provides an insight on the application of piperine as an effective bioenhancer and the superiority of a bioenhanced drug formulation over the one without a bioenhancer. This concept which is found to be beneficial, has its roots in Ayurveda-the traditional Indian system of medicine and has been applied to various drugs. It presents a fine instance of the advantage of amalgamating a traditional system with contemporary medicine.","PeriodicalId":19833,"journal":{"name":"Pharmaceutica Analytica Acta","volume":"16 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87598068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.4172/2153-2435.1000583
Mhase, B. Nanjwade, Arindam Sarkar, T. Srichana
The objective of present work is to develop and characterize dual release tablet formulation containing Metformin in extended release matrix form and Pioglitazone in immediate release form for the treatment of Diabetes mellitus. Different formulations containing Metformin HCl were manufactured using 32 factorial designs. Influences of hydrophobic carrier, hydrophilic polymer on drug release were studied. Immediate release layer of Pioglitazone was optimized using different disintegrants. All formulations were evaluated for percentage drug release and analyzed according to various release kinetic models. Optimization results indicated that release rate of Metformin is directly proportional to the levels of stearic acid (SA) and poly-ethylene-oxide (PEO). Kinetic analysis showed that formulation M6 was good releasing with f2 value of 81.08 and follows Higuchi and Peppas model with correlation coefficient value 0.9780 and 0.9910 respectively. Similarly, optimization study indicated that release of Pioglitazone is dependent on the level and type of disintegrant, formulation P5 shown highest f2 value of 84.08. Results confirmed that dual-release inlay-tablet formulation containing extended release of Metformin HCl and immediate release of Pioglitazone HCl could be developed for the treatment of Diabetes mellitus.
{"title":"Development and Evaluation of Dual Release Tablet of Metformin and Pioglitazone for the Treatment of Diabetes Mellitus","authors":"Mhase, B. Nanjwade, Arindam Sarkar, T. Srichana","doi":"10.4172/2153-2435.1000583","DOIUrl":"https://doi.org/10.4172/2153-2435.1000583","url":null,"abstract":"The objective of present work is to develop and characterize dual release tablet formulation containing Metformin in extended release matrix form and Pioglitazone in immediate release form for the treatment of Diabetes mellitus. Different formulations containing Metformin HCl were manufactured using 32 factorial designs. Influences of hydrophobic carrier, hydrophilic polymer on drug release were studied. Immediate release layer of Pioglitazone was optimized using different disintegrants. All formulations were evaluated for percentage drug release and analyzed according to various release kinetic models. Optimization results indicated that release rate of Metformin is directly proportional to the levels of stearic acid (SA) and poly-ethylene-oxide (PEO). Kinetic analysis showed that formulation M6 was good releasing with f2 value of 81.08 and follows Higuchi and Peppas model with correlation coefficient value 0.9780 and 0.9910 respectively. Similarly, optimization study indicated that release of Pioglitazone is dependent on the level and type of disintegrant, formulation P5 shown highest f2 value of 84.08. Results confirmed that dual-release inlay-tablet formulation containing extended release of Metformin HCl and immediate release of Pioglitazone HCl could be developed for the treatment of Diabetes mellitus.","PeriodicalId":19833,"journal":{"name":"Pharmaceutica Analytica Acta","volume":"7 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88461367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.4172/2153-2435.1000580
R. Maallah, A. Chtaini
Voltametric degradation of phenol was carried out at microbial electrode. This electrode is based on graphite carbon and natural phosphate modified by bacteria inserted in the phosphate matrix, the whole is covered by a polymer developed in situ on the surface. This electrode, designated subsequently by bacteria-NP-CPE, Showed stable response and was characterized with voltametric methods, as cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The experimental results revealed that the prepared electrode could be a feasible for degradation of hazardous phenol pollutants.
{"title":"Bacterial Electrode for the Oxidation and Detection of Phenol","authors":"R. Maallah, A. Chtaini","doi":"10.4172/2153-2435.1000580","DOIUrl":"https://doi.org/10.4172/2153-2435.1000580","url":null,"abstract":"Voltametric degradation of phenol was carried out at microbial electrode. This electrode is based on graphite carbon and natural phosphate modified by bacteria inserted in the phosphate matrix, the whole is covered by a polymer developed in situ on the surface. This electrode, designated subsequently by bacteria-NP-CPE, Showed stable response and was characterized with voltametric methods, as cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The experimental results revealed that the prepared electrode could be a feasible for degradation of hazardous phenol pollutants.","PeriodicalId":19833,"journal":{"name":"Pharmaceutica Analytica Acta","volume":"480-481 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77853067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：