Pakistan Veterinary Journal最新文献_第5页

Evaluation of Immunogenicity and Protective Efficacy of Eimeria maxima Immune Mapped Protein 1 with EDA Adjuvant in Chicken EDA佐剂对最大艾美耳球虫免疫定位蛋白1的免疫原性和保护作用的评价

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2020-04-01 DOI: 10.29261/pakvetj/2020.043

X. Lin

Received: Revised: Accepted: Published online: December 15, 2018 December 12, 2019 May 03, 2020 May 06, 2020 Immune mapped protein-1 (IMP1) has been shown as a protective protein associated with Eimeria (E.) maxima and cellular fibronectin extra domain A (EDA), the ligand of toll-like receptor 4 (TLR4), has the potential to be used as molecular adjuvant of the vaccine. In the present study, we estimated the protective efficacy of a subunit vaccine comprising of EDA and EmIMP1 against E. maxima challenges. For this purpose, the fusion protein EmIMP1-EDA was thoroughly purified by using over-expression of replicated E. coli of the protein. Chickens were immunized by the EmIMP1 vaccine having Freund’s adjuvant or with the EmIMP1-EDA vaccine without adjuvant. The immunization of chicken by the EmIMP1-EDA vaccine produced stronger IFN-γ secretion as compared to that in other groups (P<0.05). The result of the EmIMP1EDA vaccine was more pronounced on the parameters including weight gain, oocyst shedding, and lesion scores as compared to the EmIMP1 vaccine employing Freund’s adjuvant, but there were no statistical differences between the two groups (P>0.05). These results showed that EmIMP1-EDA fusion protein could be used as a potent subunit vaccine against E. maxima infection. ©2020 PVJ. All rights reserved

2018年12月15日2019年12月12日2020年5月03日2020年5月06日免疫定位蛋白-1 (IMP1)已被证明是一种与艾美拉球(E.) maxima和细胞纤维连接蛋白额外结构域a (EDA)相关的保护蛋白，是toll样受体4 (TLR4)的配体，具有用作疫苗分子佐剂的潜力。在本研究中，我们估计了由EDA和EmIMP1组成的亚单位疫苗对最大大肠杆菌攻击的保护功效。为此，通过过度表达复制的大肠杆菌，彻底纯化了融合蛋白EmIMP1-EDA。分别用含Freund佐剂的EmIMP1疫苗和不含佐剂的EmIMP1- eda疫苗对鸡进行免疫。EmIMP1-EDA疫苗免疫鸡后，IFN-γ分泌量显著高于其他各组(P0.05)。这些结果表明，EmIMP1-EDA融合蛋白可以作为抗大大肠杆菌感染的有效亚单位疫苗。©2020 PVJ。版权所有

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引用次数: 14

Phytochemical Analysis and In Vitro Activity of Essential Oils of Selected Plants against Salmonella enteritidis and Salmonella gallinarum of Poultry Origin 部分植物精油对家禽源肠炎沙门氏菌和鸡源沙门氏菌的体外活性及植物化学分析

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2020-04-01 DOI: 10.29261/pakvetj/2019.110

Sidra Yasmin

Received: Revised: Accepted: Published online: July 17, 2019 August 25, 2019 September 09, 2019 October 24, 2019 Antibiotic resistant Salmonella is a major threat to poultry industry and public health. Medicinal plants are an effective alternative of antibiotics for the control and treatment of multiple drug resistant Salmonella. The objective of this study was to evaluate the in-vitro activity of essential oils of some medicinal plants against multiple drug resistant Salmonella of poultry origin and to determine their active ingredients. Essential oils of Cuminum cyminum, Cinnamomum zeylanicum, Eucalyptus globulus, Allium sativum and Nigella sativa were prepared by steam distillation and their active ingredients were determined by Gas ChromatographyMass Spectrometry (GC-MS) analysis. Activity of oils against Salmonella enteritidis (n=05) and Salmonella gallinarum (n=05) was determined by well diffusion assay. Broth microdilution assay was employed to determine the minimum inhibitory concentrations of oils. Well diffusion assay revealed that C. zeylanicum and E. globulus had better activity against salmonellae (26±7.6 mm and 16±6.8 mm, respectively) as compared to C. cyminum, A. sativum and N. sativa (8±5.9, 10±6.1, 8±4.7 mm, respectively). Minimum inhibitory concentrations of C. zeylanicum and E. globulus against Salmonella were 64.1±32.1 and 68.9±32.9 μg/mL, respectively. The GC-MS analysis revealed presence of diverse phytochemicals in all essential oils. Major antimicrobial phyto-constituents of essential oils of E. globulus and C. zeylanicum were eucalyptol (82.85%) and 1R-α-Pinene (13.781%), and cinnamaldehyde (64.14%) and eugenol (8.9%), respectively. It is concluded that essential oils of C. zeylanicum and E. globulus have excellent in vitro antiSalmonella activity. It is insinuated that these extracts may be commercialized as an alternative of antibiotics for the control of Salmonellae in poultry after detailed in vivo evaluations. ©2019 PVJ. All rights reserved

收到:修订:接受:在线发布:2019年7月17日2019年8月25日2019年9月09日2019年10月24日抗生素耐药性沙门氏菌是对家禽业和公众健康的重大威胁。药用植物是控制和治疗多重耐药沙门氏菌的有效替代抗生素。本研究的目的是评价几种药用植物精油对禽源多重耐药沙门氏菌的体外活性，并确定其有效成分。采用水蒸气蒸馏法制备了Cuminum cyminum zeylanicum、Eucalyptus globulus、Allium sativum和Nigella sativa精油，并采用气相色谱-质谱(GC-MS)分析方法对其有效成分进行了测定。采用孔扩散法测定精油对肠炎沙门氏菌(n=05)和鸡沙门氏菌(n=05)的活性。采用微量肉汤稀释法测定油脂的最低抑菌浓度。孔扩散试验结果表明，zeylanicum和E. globulus对沙门氏菌的抑菌活性(分别为26±7.6 mm和16±6.8 mm)优于C. cyminum、A. sativum和N. sativa(分别为8±5.9、10±6.1和8±4.7 mm)。zeylanicum和globulus对沙门氏菌的最低抑制浓度分别为64.1±32.1和68.9±32.9 μg/mL。GC-MS分析显示，所有精油中均含有多种植物化学物质。黄姜和黄姜精油的主要抑菌成分为桉油醇(82.85%)和1R-α-蒎烯(13.781%)，肉桂醛(64.14%)和丁香酚(8.9%)。综上所述，莪术挥发油和球孢挥发油具有较好的体外抗沙门氏菌活性。据推测，经过详细的体内评价，这些提取物可能作为抗生素的替代品用于控制家禽沙门氏菌的商业化。©2019 PVJ。版权所有

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引用次数: 16

Immunocytochemical Detection of Kisspeptin Receptor and Its Association with Motility of Buffalo Bull (Bubalus bubalis) Spermatozoa Kisspeptin受体的免疫细胞化学检测及其与水牛精子活力的关系

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2020-04-01 DOI: 10.29261/pakvetj/2020.007

A. Hussain

Received: Revised: Accepted: Published online: November 11, 2019 December 11, 2019 December 15, 2019 January 21, 2020 Kisspeptin is a powerful regulator of the hypothalamic-pituitary-gonadal axis. It acts through its receptor GPR54 to regulate sexual maturation, oogenesis, spermatogenesis and fertilization. The present research was implemented to evaluate the presence of kisspeptin receptor on various regions of buffalo bull spermatozoa and to decipher its relationship with different motility parameters of the fresh spermatozoa. Standard swim-up protocol was performed on fresh ejaculates from Nili-Ravi buffalo bulls to obtain three hypothetical layers, having spermatozoa with enhanced motility and normal morphology. The progressive sperm motility was measured in each layer by using phase contrast microscopy. Methanol fixed sperm smears from each layer were processed for standard immunocytochemistry procedure for the detection of kisspeptin receptor using specific antibodies. Maximum GPR54 immunoreactivity was observed in the upper regions (head, neck/midpiece) and moderate immunoreactivity was seen in the lower region (tail) of the spermatozoa in all the three layers. No significant difference in total GPR54 expression was observed in the spermatozoa from three layers and no relationship was observed between percentage motility and GPR54 like ir. Present findings suggest that the buffalo sperm motility is not influenced by GPR54 expression on different regions of the sperm. However, robust GPR54 expression in dorsal areas of buffalo sperm, would raise possibility of a covert function of kisspeptin in the bubaline sperm biology. ©2019 PVJ. All rights reserved

接收:修改:接收:在线发布:2019年11月11日2019年12月11日2019年12月15日2020年1月21日Kisspeptin是下丘脑-垂体-性腺轴的强大调节剂。它通过受体GPR54调节性成熟、卵子发生、精子发生和受精。本研究旨在评价kisspeptin受体在水牛精子不同部位的存在，并分析其与新鲜精子不同运动参数的关系。对Nili-Ravi水牛的新鲜射精进行标准的游泳程序，以获得三层假设的精子，这些精子具有增强的运动性和正常的形态。用相差显微镜测定每层精子的进行性运动。每层甲醇固定精子涂片经标准免疫细胞化学程序处理，使用特异性抗体检测kisspeptin受体。三层精子上部(头、颈/中段)GPR54免疫活性最高，下部(尾)GPR54免疫活性中等。三层精子中GPR54的总表达量无显著差异，运动率与GPR54的表达量无显著相关。目前的研究结果表明，水牛精子的活力不受精子不同区域GPR54表达的影响。然而，GPR54在水牛精子背侧区域的强烈表达，将提高kisspeptin在雄性精子生物学中的隐蔽功能的可能性。©2019 PVJ。版权所有

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引用次数: 5

Effects of Yeast Addition to the Diet of Japanese Quails on Growth Performance, Selected Serum Parameters and Intestinal Morphology as well as Pathogens Reduction 日粮中添加酵母对日本鹌鹑生长性能、选定血清参数、肠道形态及致病菌减少的影响

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2020-04-01 DOI: 10.29261/pakvetj/2019.125

A. A. El-Wahab, R. Mahmoud, Basma H. Marghani, Hossam Gadallah

Received: Revised: Accepted: Published online: May 08, 2019 November 03, 2019 November 04, 2019 December 26, 2019 The objectives of the current study were to investigate the impact of dietary yeast (Saccharomyces cerevisiae) supplementation on growth performance, some serum parameters, intestinal morphology and pathogens reduction in Japanese quails. In total, 300 d-old Japanese quails were randomly allocated to 5 dietary groups (6 replicates of 10 Japanese quails per pen). At beginning of the experiment (d 14), Japanese quails fed a basal diet without contain any yeast while, the other treatments provided basal diet plus 0.5, 1.5, 2.5 and 3.5% yeast on feed basis. The results showed that dietary supplementation of yeast, particularly at a level of inclusion of 3.5% on feed basis, resulted in highest BW for Japanese quails (P<0.05). Japanese quails fed diets supplemented with yeast reduced (P<0.05) cholesterol and triglycerides concentrations in serum compared to control. Dietary yeast supplementation at 3.5% reduced (P<0.05) interleukin 1 β (IL-1 β), IL-6 and TNF-α levels in serum compared to those fed dietary yeast at level of 0.5% or fed non supplemented diets. Feeding yeast at level of 3.5% led to increase in duodenal villus height significantly compared to other treatments. Japanese quails fed yeast supplemented diets at 2.5 or 3.5% reduced E. coli (P<0.05) and C. perfringens CFU counts in excreta than those fed non supplemented diet. It is concluded that dietary yeast supplementation of Japanese quails improved growth rate as a result of an increase in villus height and a reduce in the counts of E. coli and C. perfringens. ©2019 PVJ. All rights reserved

本研究的目的是研究饲粮中添加酵母(Saccharomyces cerevisiae)对日本鹌鹑生长性能、部分血清参数、肠道形态和病原体减少的影响。试验300只d龄日本鹌鹑，随机分为5个饲粮组(6个重复，每栏10只)。试验开始(第14天)，饲喂不添加酵母的基础饲粮，其余试验组分别在饲料基础上添加0.5、1.5、2.5和3.5%酵母。结果表明，饲粮中添加酵母，尤其是在饲料基础上添加3.5%时，日本鹌鹑的体重最高(P<0.05)。与对照组相比，添加酵母降低了日本鹌鹑血清胆固醇和甘油三酯浓度(P<0.05)。饲粮中添加3.5%酵母与饲粮中添加0.5%酵母或未添加酵母相比，降低了血清中白细胞介素1 β (IL-1 β)、IL-6和TNF-α水平(P<0.05)。与其他处理相比，饲喂3.5%水平的酵母显著增加了十二指肠绒毛高度。饲粮中添加2.5%或3.5%酵母的日本鹌鹑排泄物中大肠杆菌和产气荚膜杆菌CFU数量显著低于未添加酵母的日本鹌鹑(P<0.05)。由此可见，日粮中添加酵母可提高日本鹌鹑的生长速度，其原因是绒毛高度增加，大肠杆菌和产气荚膜杆菌数量减少。©2019 PVJ。版权所有

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引用次数: 7

Effect of Grape Seed Extract on Tibial Dyschondroplasia Incidence, Liver Weight, and Tibial Angiogenesis in Chickens 葡萄籽提取物对鸡胫骨软骨发育不良发生率、肝脏重量和胫骨血管生成的影响

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2020-04-01 DOI: 10.29261/pakvetj/2019.109

Hammad Qamar

Received: Revised: Accepted: Published online: July 28, 2019 October 12, 2019 October 13, 2019 October 21, 2019 Among avian metabolic disorders, Tibial dyschondroplasia (TD) has a wide prevalence worldwide in chickens that are identified by non-mineralization and avascularization of the growth plate (GP). Grape seed extract (GSE) reduces the inflammation and oxidative stress by scavenging free oxygen specie and has some anti-cancer and angiogenic activity. This study was carried out to evaluate the role of GSE for the therapy of the chickens affected with TD. Arbor Acres broiler chicks (n=240) weighing 47±6g were fed starter feed till first 3 days and then portioned equally into three groups (Control, TD and GSE group; n=80 each). Thiram was incorporated in the feed of TD and GSE chicks until day 6 for inducing TD (50 mg/ kg of feed), whereas usual feed was offered to control group. All the birds in TD and GSE group showed the symptoms of TD on day 7 and GSE group was given GSE (50 mg/kg/day) intraperitoneally till the termination of the experiment. Chickens fed with thiram-feed exhibited high TD score and GP measurement. After the GSE treatment, the TD score dropped remarkably and, inflammation and TD lesions reduced. Moreover, body weight gain and liver weight were increased in comparison with TD chicks after application of GSE. GSE supplementation alleviated the adverse effects of thiram and increase in angiogenesis was observed. In conclusion, GSE recovered the TD affected broilers through downregulating the HIF-1α, Hsp90 and CA2 expressions. ©2019 PVJ. All rights reserved

2019年7月28日2019年10月12日2019年10月13日2019年10月21日2019年10月21日在禽类代谢疾病中，胫骨软骨发育不良(Tibial dyschondroplasia, TD)在全球范围内广泛流行，以生长板(growth plate, GP)的非矿化和无血管化为特征。葡萄籽提取物(GSE)通过清除游离氧来减轻炎症和氧化应激，并具有一定的抗癌和血管生成活性。本研究旨在评价GSE对鸡TD的治疗作用。选取240只体重为47±6g的爱拔益加肉仔鸡，饲喂初饲料至前3 d，然后随机分为3组(对照组、TD组和GSE组;n = 80)。在TD和GSE雏鸡的饲料中添加Thiram (50 mg/ kg饲料)，直至第6天诱导TD，对照组饲喂普通饲料。试验第7天，TD组和GSE组均出现TD症状，GSE组给予GSE (50 mg/kg/d)腹腔注射至试验结束。饲粮中添加三聚氰胺的鸡具有较高的TD评分和GP测量值。经GSE治疗后，TD评分明显下降，炎症和TD病变减少。此外，与TD雏鸡相比，GSE的增重和肝重均有所提高。GSE的补充减轻了thiram的不良反应，并增加了血管生成。综上所述，GSE通过下调HIF-1α、Hsp90和CA2的表达来恢复TD病肉鸡。©2019 PVJ。版权所有

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引用次数: 10

Effect of Lactobacillus gallinarum PL 53 Supplementation on Xylose Absorption and Intestinal Morphology in Broilers Challenged with Campylobacter jejuni 添加鸡乳杆菌PL53对空肠弯曲杆菌攻击肉鸡木糖吸收和肠道形态的影响

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2020-04-01 DOI: 10.29261/pakvetj/2020.011

Madiha Khan

Received: Revised: Accepted: Published online: July 30, 2019 October 08, 2019 October 16, 2019 January 25, 2020 A feeding trial of indigenous probiotic lactobacilli strains including Lactobacillus gallinarum PL 53, L. paracasei PL 120 and L. gallinarum PL 149 was conducted to monitor the effect on intestinal absorption capacity and histological changes in broiler chicks challenged with Campylobacter jejuni. A total of 45, day old chicks were randomly assigned to nine experimental groups (5 birds/ group). Group A was negative and B positive control while group C, D and E prevention model, F, G and H treatment model and group I antibiotic control. Groups other than negative control received Campylobacter jejuni (10 CFUs/bird) challenge on day 14 by oral gavage. The groups of prevention model received lactobacilli (~10 CFUs/bird) strains from day 1-35 and treatment model received lactobacilli from day 15-35. The absorption capacity of intestine was monitored by concentration of D-xylose in plasma (0.5 and 1 hour post administration). The probiotic group L. gallinarum PL 53 (group C) significantly enhanced Dxylose absorption capacity as compared to control groups (59.69±2.07mg/dL) in both the prevention (72.83±1.20mg/dL) and treatment (71.11±2.27mg/dL) models. Intestinal morphology was observed by measuring villi length, width, crypt depth and surface area of the small intestine. PL 53 increased the villi length of ileum (930±4.00μm), jejunum (890±8.00μm) and duodenum (1350±4.00μm). The group which received L. gallinarum PL 53 strain had maximum surface area of the intestinal segments with 0.41±0.03mm, 0.45±0.03mm and 0.72±0.02mm of ileum, jejunum and duodenum, respectively. In conclusion, the PL 53 L. gallinarum may improve the gut performance and absorption capacity of broilers. ©2019 PVJ. All rights reserved

接收：修订：接受：在线发布：2019年7月30日2019年10月8日2019年1月16日2020年1月25日本地益生菌乳酸杆菌菌株的饲养试验，包括鸡乳杆菌PL 53，研究了副干酪乳杆菌PL 120和鸡乳杆菌PL 149对空肠弯曲杆菌攻击肉鸡肠道吸收能力和组织学变化的影响。将45只日龄雏鸡随机分为9个实验组（每组5只）。A组为阴性和B阳性对照，C、D和E组为预防模型，F、G和H组为治疗模型，I组为抗生素对照。阴性对照组以外的其他组在第14天通过口服管饲法接受空肠弯曲杆菌（10 CFU/只）攻击。预防模型组从第1-35天开始接受乳酸杆菌（~10 CFU/只）菌株，治疗模型从第15-35天开始接受乳杆菌。通过血浆中D-木糖的浓度监测肠道的吸收能力（给药后0.5和1小时）。在预防（72.83±1.20mg/dL）和治疗（71.11±2.27mg/dL）模型中，与对照组（59.69±2.07mg/dL）相比，益生菌组鸡乳杆菌PL 53（C组）显著增强了D木糖吸收能力。通过测量小肠绒毛的长度、宽度、隐窝深度和表面积来观察肠道形态。PL53增加了回肠（930±4.00μm）、空肠（890±8.00μm）和十二指肠（1350±4.00µm）的绒毛长度。总之，PL53L.gallinarum可提高肉鸡的肠道性能和吸收能力。©2019 PVJ。保留所有权利

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引用次数: 3

Molecular Characterization and Phylogenetic Analysis of Babesia Species Isolated from Domestic Cattle 家牛巴贝虫分离种的分子特征及系统发育分析

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2020-04-01 DOI: 10.29261/pakvetj/2019.003

Rimsha Farooq, M. Hafeez, M. Oneeb, A. Rafique, K. Ashraf, F. Aslam, Nauman Rauf, Kiran Khalid, Farrukh Bilal, S. Mahmood, A. Sattar

Received: Revised: Accepted: Published online: June 15, 2019 September 25, 2019 January 06, 2020 January 07, 2020 Babesiosis is a key tick born disease caused by the protozoal parasites belonging to the genus Babesia, cosmopolitan in nature and infecting enormous range of large ruminants. Study was intended to determine the presence of local isolates of Babesia species (B. bovis, B. bigemina) their molecular characterization and phylogenetic relationship in cattle (district Narowal) Punjab. Blood samples were collected from 200 suspected cattle, from seven villages of selected district. DNA was extracted by using DNA zole, Gene-all Kit, manual method and subjected to PCR for amplification using specific RLB and Universal Primers. Quantification of protozoan parasite was performed by RT PCR using Solis biodine kit. A total of 36 out of 200 animal samples, were found positive by microscopy. PCR positive samples (17) were amplified and bands of strength 520-bp and 800-bp with specific RLB and for universal primers respectively were obtained for Babesia species. These 17 samples were sent for sequencing and by using Clustal W; Bioedit software with Neighbor-joining method phylogenetic tree was established. Current study reported the presence of B. bigemina i.e. 18% (conventional method) in large ruminants of district Narowal, Pakistan, and confirmation by (molecular characterization) PCR (8.5%) and phylogenetic relationship. Phylogenetic results showed that our study has homology with Babesia bigemina strain. ©2020 PVJ. All rights reserved

接收日期：修订日期：接受日期：在线发布日期：2019年6月15日至2019年9月25日至2020年1月6日巴贝虫病是一种由巴贝虫属原生动物寄生虫引起的主要蜱生疾病，自然界普遍存在，感染范围广泛的大型反刍动物。该研究旨在确定巴贝斯虫物种（B.bovis，B.bigemina）的本地分离株的存在——它们在旁遮普省（Narowal区）牛中的分子特征和系统发育关系。从选定地区七个村庄的200头疑似牛身上采集了血样。使用DNA唑、基因全试剂盒、手动法提取DNA，并使用特异性RLB和通用引物进行PCR扩增。使用Solis biodine试剂盒通过RT-PCR进行原生动物寄生虫的定量。在200份动物样本中，共有36份通过显微镜检查呈阳性。扩增PCR阳性样品（17），并分别获得具有特异性RLB和通用引物的强度为520bp和800bp的巴贝虫条带。将这17个样本送去测序，并使用Clustal W；建立了基于邻居连接法的生物编辑软件系统发育树。目前的研究报告了在巴基斯坦Narowal地区的大型反刍动物中存在B.bigemina，即18%（传统方法），并通过（分子表征）PCR（8.5%）和系统发育关系进行了证实。系统发育结果表明，本研究与双联巴贝虫菌株具有同源性。©2020 PVJ。保留所有权利

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引用次数: 6

Postpartum Cyclicity of Holstein-Friesian Crossbred Cows Shows Relation with Serum Biochemical Profiles during 45-60 Days Postpartum 荷母杂交奶牛产后周期与产后45 ~ 60 d血清生化指标的关系

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2020-04-01 DOI: 10.29261/pakvetj/2019.122

A. Uddin, M. Atikuzzaman, M. S. Islam, M. Hossain

Received: Revised: Accepted: Published online: September 23, 2019 November 20, 2019 November 22, 2019 December 06, 2019 Reproductive performance of dairy cows is greatly affected by energy, protein and mineral profiles, which is also connected to postpartum anestrus. The present study was conducted during the period from December 2018 to May 2019 on 30 local x Holstein-Friesian crossbred lactating cows with parity 1-5 and body condition score of 2.0-3.5. This study aimed to compare serum glucose, cholesterol, triglycerides, total proteins, urea nitrogen, calcium, phosphorus and magnesium concentrations between anestrus and normal cyclic crossbred cows during 45 to 60 days postpartum. Automated biochemical analyzer was employed to measure these concentrations, using 15 cows in each group. The serum of postpartum anestrus cows contained significantly lower (P<0.05) concentrations of glucose, total proteins, calcium and magnesium compared to normal cyclic cows, while urea nitrogen concentration was significantly increased (P<0.05) in the postpartum anestrus cows. However, concentrations of other serum biochemical metabolites did not differ between cows of the two groups. These results suggest that blood glucose, total proteins, calcium, magnesium and urea nitrogen might have an effect on the cyclicity of Holstein-Friesian crossbred cows during 45 to 60 days postpartum. However, further studies are recommended to explore the underlying mechanism for the role of these metabolites in the cyclicity of postpartum cows. ©2019 PVJ. All rights reserved

接收日期：修订日期：接受日期：在线发布日期：2019年9月23日2019年11月20日2019年十一月22日2019年12月06日奶牛的繁殖性能受能量、蛋白质和矿物质含量的影响很大，这也与产后麻醉有关。本研究于2018年12月至2019年5月期间对30头产程为1-5、身体状况评分为2.0-3.5的当地x荷斯坦-弗里斯杂交泌乳奶牛进行。本研究旨在比较产后45至60天内无妊娠期和正常周期杂交奶牛的血糖、胆固醇、甘油三酯、总蛋白、尿素氮、钙、磷和镁浓度。使用自动生化分析仪测量这些浓度，每组使用15头奶牛。与正常周期奶牛相比，产后无挤压奶牛血清中葡萄糖、总蛋白、钙和镁的浓度显著降低（P<0.05），而尿素氮的浓度显著升高（P<0.05）。然而，其他血清生化代谢产物的浓度在两组奶牛之间没有差异。这些结果表明，血糖、总蛋白、钙、镁和尿素氮可能对荷斯坦-弗里斯杂交奶牛产后45至60天的周期性产生影响。然而，建议进行进一步的研究，以探索这些代谢产物在产后奶牛周期性中作用的潜在机制。©2019 PVJ。保留所有权利

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引用次数: 0

Casticin Alleviates Testicular and Spermatological Damage Induced by Cisplatin in Rats 蓖麻素减轻顺铂致大鼠睾丸和精子损伤

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2020-04-01 DOI: 10.29261/pakvetj/2019.127

M. Ijaz

Received: Revised: Accepted: Published online: September 11, 2019 September 19, 2019 December 02, 2019 December 31, 2019 Cancer is one of the leading causes of mortality in animals. Cisplatin (CP) is a chemotherapeutic drug, which is used in treatment of several types of solid tumors. However, it causes numerous side effects on multiple organs, specifically to testicular tissues. This study elucidated the alleviative effects of casticin (CAS) on CP induced damages in testes of rats. Twenty-four adult male Sprague Dawley rats were used and divided into four groups. Each group consisted of six animals. These four groups were treated as followed: Control group (0.9% saline), CP treated group (10 mg/kg. b.wt.), CP+CAS treated group (10 mg/kg. b.wt. CP and 50 mg/kg. b.wt. CAS) and CAS treated group (50 mg/kg. b.wt.). The treatment duration was one week. Biochemical, histomorphometric, hormonal and spermatogenic profiles were assessed. Results of present investigation indicated that CP treatment significantly decreased the activities of catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) and glutathione reductase (GSR), while thiobarbituric acid reactive substances (TBARS) level was increased. Furthermore, the count of germ cells and Leydig cells, epithelial height and diameter of seminiferous tubules, plasma and intratesticular testosterone concentrations as well as daily sperm production (DSP) along with its efficiency were decreased. Whereas, luminal diameter and interstitial spaces were increased. CAS is a flavonoid, which exhibits antioxidant and reactive oxygen species (ROS) scavenging properties. Therefore, it restored these damages to some degree by attenuating CP induced toxicity in testicular tissues. ©2019 PVJ. All rights reserved

接收:修订:接收:在线发布:2019年9月11日2019年9月19日2019年12月02日2019年12月31日癌症是动物死亡的主要原因之一。顺铂(CP)是一种化疗药物，用于治疗几种类型的实体瘤。然而，它会对多个器官产生许多副作用，特别是睾丸组织。本研究阐明了蓖麻素(CAS)对CP致大鼠睾丸损伤的缓解作用。选取24只成年雄性斯普拉格·道利大鼠，分为四组。每组由6只动物组成。各组处理如下:对照组(0.9%生理盐水)，CP处理组(10 mg/kg)。b.wt.)， CP+CAS处理组(10 mg/kg)。b.wt。CP和50 mg/kg。b.wt。CAS)和CAS处理组(50 mg/kg)。b.wt)。治疗时间为1周。评估了生物化学、组织形态学、激素和生精特征。结果表明，CP处理显著降低了过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、过氧化物酶(POD)和谷胱甘肽还原酶(GSR)活性，提高了硫代巴比妥酸活性物质(TBARS)水平。生殖细胞和间质细胞数量、精小管上皮高度和直径、血浆和睾丸内睾酮浓度、日精子产生量(DSP)及其效率均降低。而管腔直径和间隙增大。CAS是一种类黄酮，具有抗氧化和清除活性氧(ROS)的特性。因此，它通过减轻CP对睾丸组织的毒性，在一定程度上恢复了这些损伤。©2019 PVJ。版权所有

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引用次数: 17

Autosomal Cholesterol Deficiency in a Holstein Calf 荷斯坦牛常染色体胆固醇缺乏症

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2020-04-01 DOI: 10.29261/pakvetj/2019.120

J. Jacinto, M. Bolcato, Drögemüller Cord, A. Gentile, G. Militerno

Cholesterol deficiency (CD) is an autosomal recessive defect in Holstein cattle caused by a mutation in the apolipoprotein B gene ( APOB ). This paper reports the clinical and pathological phenotype of a case of CD in a 5-months-old Holstein calf. Retarded growth, chronic, intermittent diarrhea, stomatitis, hypocholesterolemia and low blood triglycerides concentrations were the most important clinical and ancillary findings. Histopathologically, inflammation of the digestive organs was the most evident sign. Blood from the patient, the dam, sister1, sister2 and semen of the sire were tested for APOB mutation: the calf resulted homozygous, whereas the dam and the sire resulted heterozygous carriers. Both sisters were APOB mutation free. Cholesterol deficiency should be considered in the differential diagnosis of chronic diarrhea and failure to thrive in Holstein calves.

胆固醇缺乏症(CD)是由载脂蛋白B基因(APOB)突变引起的荷斯坦牛常染色体隐性缺陷。本文报告了一例5个月大荷斯坦小牛乳糜泻的临床和病理表型。生长迟缓、慢性间歇性腹泻、口炎、低胆固醇血症和低血甘油三酯浓度是最重要的临床和辅助表现。组织病理学上，消化器官的炎症是最明显的征象。对患者、母马、母马、母马、母马和母马的精液进行APOB突变检测，小牛为纯合子，母马和母马为杂合子。两姐妹均无APOB突变。在荷斯坦犊牛慢性腹泻和发育不良的鉴别诊断中，应考虑胆固醇缺乏。

引用次数: 1