Pakistan Veterinary Journal最新文献_第10页

Effect of Newly Characterized Probiotic Lactobacilli on Weight Gain, Immunomodulation and Gut Microbiota of Campylobacter jejuni Challenged Broiler Chicken 新型益生菌乳酸杆菌对空肠弯曲杆菌挑战肉鸡增重、免疫调节和肠道微生物群的影响

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2019-10-01 DOI: 10.29261/pakvetj/2019.051

Madiha Khan

Received: Revised: Accepted: Published online: January 08, 2019 February 12, 2019 February 13, 2019 April 06, 2019 The aim of the present study was to determine the competitive exclusion of Campylobacter jejuni in poultry gut by three potential probiotic lactobacilli strains. A total of 135 birds of one day age were randomly divided into nine groups. The groups of prevention model received respective strains from day 1-35 and treatment model received lactobacilli from day 15-35 (after challenging with C. jejuni). These groups were given lactobacilli (~10 CFUs) while challenged with C. jejuni on day 14 by oral gavage (10 CFUs/bird). There were three control groups including A, B and I. Negative control (A) did not receive any treatment, C. jejuni was given to group B and group I was given Enrofloxacin formulation. Cloacal swabs were collected from birds of each group before and after challenge while the ceca were collected from birds after slaughtering (on day 35) for enumeration of aerobic bacteria, coliform, C. jejuni, lactobacilli and Bifidobacterium on selective agar plates. Effect of lactobacilli on weight gain and New Castle Disease vaccine (NDV) titer were also evaluated. Probiotic strain Lactobacillus gallinarum PL 53 considerably decreased log10 values of aerobic plate count (3.19±0.66), coliform count (2.83±0.22) and C. jejuni (3.98±0.77) in poultry. The probiotics also enhanced Lactobacillus and Bifidobacterium spp counts (~2-3 log increase). Treatment group C had maximum weight gain (1994±188.32g) and geometric mean titer (274.4) on day 28. The results of in-vivo experiments concluded that probiotic administration may be effective for targeted mitigation of C. jejuni in broiler birds. ©2019 PVJ. All rights reserved

接收：修订：接受：在线发布：2019年1月8日2019年2月12日2019年02月13日2019年04月06日本研究的目的是通过三种潜在的益生菌乳酸杆菌菌株确定家禽肠道中空肠弯曲杆菌的竞争性排除。将135只一日龄的鸟类随机分为9组。预防模型组从第1-35天接受各自的菌株，治疗模型从第15-35天接受乳酸杆菌（用空肠弯曲菌攻击后）。这些组在第14天用空肠弯曲菌经口灌胃攻击时给予乳酸杆菌（~10 CFU）（10 CFU/只）。对照组分为A、B和I三组。阴性对照组（A）未接受任何治疗，B组给予空肠弯曲菌，I组给予恩诺沙星制剂。在攻击前后从每组鸟类中采集泄殖腔拭子，而在屠宰后（第35天）从鸟类中采集盲肠，在选择性琼脂平板上计数需氧菌、大肠杆菌、空肠弯曲菌、乳酸杆菌和双歧杆菌。还评估了乳酸杆菌对体重增加和新城疫疫苗（NDV）滴度的影响。益生菌菌株鸡乳杆菌PL 53显著降低了家禽需氧菌平板计数（3.19±0.66）、大肠菌群计数（2.83±0.22）和空肠弯曲菌（3.98±0.77）的log10值。益生菌还提高了乳酸杆菌和双歧杆菌的计数（约2-3 log增加）。治疗组C在第28天出现最大体重增加（1994±188.32g）和几何平均滴度（274.4）。体内实验结果表明，益生菌给药可能对肉鸡空肠弯曲菌的靶向缓解有效。©2019 PVJ。保留所有权利

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引用次数: 11

Immunohistochemical and Histological Features of a Spontaneous Leydig Cell Tumour in a Rat 大鼠自发性Leydig细胞肿瘤的免疫组织化学和组织学特征

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2019-10-01 DOI: 10.29261/pakvetj/2019.075

R. Ciaputa, Adam Szymerowski, I. Janus, P. Prządka, M. Kandefer-Gola, M. Nowak

Received: Revised: Accepted: Published online: December 30, 2018 February 20, 2019 April 16, 2019 May 25, 2019 Neoplastic testicular lesions are diagnosed increasingly frequently in dogs and humans. In rats, spontaneous testicular tumours, particularly Leydig cell tumours, are very rare. The aim of the study was to carry out a histological and immunohistochemical analysis of a Leydig cell tumour in a rat as well as to present the similarities and differences in the expression of the proteins used to diagnose this type of tumour in dogs and men. Following the histopathological and immunohistochemical analysis (including antibodies against vimentin, calretinin, inhibin-α, β catenin and E-cadherin), significant similarities were found in the level of expression of the studied cell markers of the rat, dog and male leydigoma This indicates their usefulness as diagnostic markers of testicle tumours both in humans and animals. ©2019 PVJ. All rights reserved

收到:修订:接受:在线发布:2018年12月30日2019年2月20日2019年4月16日2019年5月25日在狗和人类中，睾丸肿瘤的诊断越来越频繁。在大鼠中，自发性睾丸肿瘤，特别是间质细胞肿瘤，是非常罕见的。这项研究的目的是对大鼠的间质细胞肿瘤进行组织学和免疫组织化学分析，并提出用于诊断狗和人这种类型肿瘤的蛋白质表达的异同。通过组织病理学和免疫组织化学分析(包括抗vimentin、calretinin、inhibin-α、β catenin和E-cadherin的抗体)，发现大鼠、狗和雄性leygooma细胞标记物的表达水平有显著的相似性，这表明它们作为人类和动物睾丸肿瘤的诊断标记物的有用性。©2019 PVJ。版权所有

引用次数: 2

Antidiabetic Activity of Date Seed Methanolic Extracts in Alloxan-Induced Diabetic Rats 椰枣籽甲醇提取物对四氧嘧啶糖尿病大鼠的抗糖尿病作用

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2019-10-01 DOI: 10.29261/pakvetj/2019.099

J. Sharifi‐Rad, S. A. Ayatollahi, Navid Baghalpour

Received: Revised: Accepted: Published online: January 30, 2019 June 23, 2019 August 22, 2019 September 11, 2019 Phoenix dactylifera. L. )date palm ( is one of the main fruits in North of Africa and Middle East. The date seeds are considered as wastes despite of their medicinal properties. Different seed preparations are used as expectorant, anti-diarrheic, hypoglycemic, tonic, and aphrodisiac agents. We established the anti-diabetic and antilipidemic activities of methanolic extracts of date seed in alloxan-induced diabetic rats. Animals were divided in to 6 groups (each group contained six animals), comprising of control group and diabetic rats. Animals were treated with different amounts of extract. To assess the hypolipidemic, and anti-hyperglycemic activity, cholesterol serum levels, low density lipoprotein (LDL), high density lipoprotein (HDL), creatinine, urea and alkaline phosphatase were measured in serum of the treated animals. The anti-diabetic potential was investigated through the levels of glucose and body weight. Moreover, to assess the extract’s safety, acute toxicity test was performed. In comparison with control group, significant reductions were observed in LDL, cholesterol, as well as blood glucose levels in diabetic rats that received date seed extract. Extract supplemented diabetic rats also showed a better tolerance to glucose, as well as reduced amounts of creatinine, urea, and alkaline phosphatase in their serum samples. In addition, no toxicity (acute toxicity), was detected even after high dosage of extract administration. Our results confirmed the anti-lipidemic and anti-diabetic potentials of date seed methanolic extract in diabetic rats. ©2019 PVJ. All rights reserved

接收：修订：接受：在线发布：2019年1月30日2019年6月23日2019年8月22日2019年9月11日凤凰指。L.）椰枣（是非洲北部和中东地区的主要水果之一。椰枣种子尽管具有药用特性，但仍被认为是废物。不同的种子制剂被用作祛痰、抗腹泻、降血糖、滋补和壮阳剂。我们建立了椰枣种子甲醇提取物对四氧嘧啶诱导的糖尿病大鼠的抗糖尿病和抗血脂活性再分为6组（每组6只动物），包括对照组和糖尿病大鼠。用不同量的提取物处理动物。为了评估降脂和抗高血糖活性，测量了治疗动物血清中的胆固醇血清水平、低密度脂蛋白（LDL）、高密度脂蛋白、肌酐、尿素和碱性磷酸酶。通过血糖和体重水平来研究抗糖尿病的潜力。此外，为了评估提取物的安全性，进行了急性毒性试验。与对照组相比，在接受椰枣籽提取物的糖尿病大鼠中，观察到LDL、胆固醇和血糖水平显著降低。补充提取物的糖尿病大鼠对葡萄糖也表现出更好的耐受性，血清样本中的肌酐、尿素和碱性磷酸酶含量也有所降低。此外，即使在高剂量的提取物给药后也未检测到毒性（急性毒性）。我们的研究结果证实了椰枣籽甲醇提取物在糖尿病大鼠中的抗脂和抗糖尿病潜力。©2019 PVJ。保留所有权利

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引用次数: 12

Effect of Recombinant Bovine Somatotropin (rbST) and Oxytocin on Health Biomarkers, Reproductive Performance and Milk Composition of Nili-Ravi Buffaloes (Bubalus bubalis) 重组牛生长激素(rbST)和催产素对尼利-拉维水牛健康生物指标、繁殖性能和乳成分的影响

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2019-10-01 DOI: 10.29261/pakvetj/2019.096

R. Shahid

Received: Revised: Accepted: Published online: October 21, 2018 August 04, 2019 August 06, 2019 September 05, 2019 In Pakistan recombinant bovine somatotropin (rbST) is commonly used to increase milk production in dairy animals. That is why this study was conducted to check its effect on health and production in buffaloes. Selected buffaloes were divided into groups (n=10) on the basis of body condition scores (BCS) i.e. (Low) BCS 1-3, (Medium) BCS 4-6 and (High) BCS 7-9, while ten animals served as control. All the groups received rbST (Boostin-250) 35 mg on alternate days and oxytocin 1 ml (10 I.U), starting on day 70 postpartum till the end of lactation. Blood samples were collected from each animal and serum was separated. Milk samples were used for milk composition parameters. The data on reproductive performance parameters was obtained from the farm record. The results indicated that mean serum Alanine aminotransferase (ALT), Aspartate aminotransferase (AST) and total oxidant status (TOS) values were higher (P≤0.01) during summer, while total antioxidant status showed opposite trend. Mean serum AST and TOS values were significantly (P≤0.01) higher in medium and high BCS buffaloes. Percentage of pregnant buffaloes was high in the high BCS group during summer. Mean milk fat contents were higher (P≤0.01) in buffaloes of high BCS while mean milk protein and lactose contents were significantly (P≤0.01) higher in medium BCS buffaloes. Fat, protein, lactose and solid not-fat (SNF) contents were higher during spring than summer. Mean milk lactose and SNF contents were higher (P≤0.01) in high than low and medium milk production groups. In conclusion, milk production was increased during summer and with rbST and decreased with oxytocin during spring, rbST and oxytocin treatments showed adverse effects on general health of Nili-Ravi buffaloes. ©2019 PVJ. All rights reserved

接收:修订:接受:在线发布:2018年10月21日2019年8月04日2019年8月06日2019年9月05日在巴基斯坦，重组牛生长激素(rbST)通常用于提高奶牛的产奶量。这就是为什么要进行这项研究，以检查它对水牛健康和生产的影响。选取水牛按体况评分(BCS)分为(低)BCS 1-3、(中)BCS 4-6、(高)BCS 7-9组(n=10)，对照组10头。各组从产后第70天开始至哺乳期结束，每隔1天给予rbST (boostin250) 35 mg，催产素1 ml (10 iu)。从每只动物身上采集血样并分离血清。牛奶样品用于牛奶成分参数。繁殖性能参数数据来源于养殖场记录。结果表明:夏季平均血清谷丙转氨酶(ALT)、谷草转氨酶(AST)和总氧化状态(TOS)值较高(P≤0.01)，总抗氧化状态呈相反趋势;中、高BCS水牛血清AST和TOS平均值显著(P≤0.01)升高。在夏季，高BCS组的怀孕水牛百分比很高。高BCS水牛的平均乳脂含量较高(P≤0.01)，中等BCS水牛的平均乳蛋白和乳糖含量极显著(P≤0.01)高于高BCS水牛。脂肪、蛋白质、乳糖和固体不脂肪(SNF)含量春季高于夏季。高产奶量组平均乳糖和SNF含量高于低、中产奶量组(P≤0.01)。综上所述，夏季和rbST处理能提高奶牛的产奶量，而春季催产素处理能降低产奶量，rbST和催产素处理对Nili-Ravi水牛的总体健康状况有不利影响。©2019 PVJ。版权所有

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引用次数: 2

Histometerical and Morphological Studies of Digestive Tract and Associated Glands in Domestic Pigeon (Columba livia) with Regard to Age 家鸽(Columba livia)消化道及相关腺体随年龄的组织学形态学研究

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2019-10-01 DOI: 10.29261/pakvetj/2019.088

R. Kausar, S. Raza, M. Hussain, S. U. K. Bahadur

引用次数: 6

LRRK2 Kinase Plays an Important Role in the Intracellular Survival of Brucella abortus 2308 in Murine Macrophages and in a Mouse Infection Model LRRK2激酶在小鼠巨噬细胞和小鼠感染模型中流产布鲁氏菌2308细胞内存活中起重要作用

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2019-10-01 DOI: 10.29261/pakvetj/2019.050

Jihai Yi

Received: Revised: Accepted: Published online: December 28, 2018 February 23, 2019 March 08, 2019 April 06, 2019 Brucella species are facultative, intracellular bacteria that cause serious diseases in animals and people. Persistent survival inside macrophages is a major cause of chronic infections with Brucella. Leucine-rich repeat kinase 2 (LRRK2), a large multidomain protein kinase, is associated with Parkinson’s disease, chronic inflammation and intracellular infections. Hence, we monitored the activation of the LRRK2 kinase in Brucella-infected cells and evaluated the function of LRRK2 kinase in the intracellular survival of Brucella. Our results show that Brucella abortus 2308 activated the LRRK2 kinase and that the kinase activity was inhibited by a LRRK2-specific inhibitor in a dose-dependent manner. LRRK2 silencing significantly increased the Th1 immune response and reduced the replication of Brucella abortus 2308, both in vitro and in vivo. LRRK2 also enhanced the phosphorylation level of Akt, thereby inhibiting Akt-mediated humoral immune responses during Brucella infection. Collectively, these findings confirm that LRRK2 acts to reduce innate immune responses by activation of PI3K-Akt pathway, thereby contributing to the intracellular survival of Brucella abortus 2308 in murine macrophages and in a mouse infection model. Therefore, LRRK2 kinase play a key role in infections with Brucella abortus 2308, which will provide new insights into the pathogenic mechanisms used by Brucella. ©2019 PVJ. All rights reserved

接收:修订:接收:在线发布:2018年12月28日2019年2月23日2019年3月08日2019年4月06日布鲁氏菌是一种兼性的细胞内细菌，可引起动物和人类的严重疾病。巨噬细胞内持续存活是布鲁氏菌慢性感染的主要原因。富亮氨酸重复激酶2 (LRRK2)是一种大型多结构域蛋白激酶，与帕金森病、慢性炎症和细胞内感染有关。因此，我们监测了布鲁氏菌感染细胞中LRRK2激酶的激活情况，并评估了LRRK2激酶在布鲁氏菌细胞内存活中的作用。我们的研究结果表明，流产布鲁氏菌2308激活了LRRK2激酶，并且该激酶活性被LRRK2特异性抑制剂以剂量依赖的方式抑制。LRRK2沉默显著增加Th1免疫应答，减少流产布鲁氏菌2308在体外和体内的复制。LRRK2还增强了Akt的磷酸化水平，从而抑制了布鲁氏菌感染期间Akt介导的体液免疫反应。综上所述，这些发现证实LRRK2通过激活PI3K-Akt通路来降低先天免疫应答，从而促进了流产布鲁氏菌2308在小鼠巨噬细胞和小鼠感染模型中的细胞内存活。因此，LRRK2激酶在流产布鲁氏菌2308感染中发挥了关键作用，这将为布鲁氏菌的致病机制提供新的见解。©2019 PVJ。版权所有

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引用次数: 1

Pharmacokinetics of Cefquinome in Layer Birds Following Intramuscular and Intravenous Administration 肌肉和静脉给药后头孢醌在蛋鸡体内的药代动力学

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2019-10-01 DOI: 10.29261/pakvetj/2019.065

R. Saleem

Received: Revised: Accepted: Published online: February 19, 2019 April 02, 2019 April 14, 2019 April 26, 2019 Cefquinome is approved for use against many bacterial infections in a number of animal species. This study was conducted to evaluate the pharmacokinetics of Cefquinome after intramuscular (IM) and intravenous (IV) administration in layer birds for its safety evaluation. Twelve healthy layer birds were randomly allocated in two equal groups. Each group was administered with 5mg/kg b.w. of Cefquinome by intramuscular and intravenous routes. Highly sensitive high performance liquid chromatography with ultraviolet detection (HPLC-UV) method was developed for quantification of Cefquinome in layer bird’s plasma with >80% recovery. The Limit of detection and quantification were 0.02 and 0.05μg/ml, respectively. The pharmacokinetic data showed that mean Area under the curve (AUC) after IM and IV administration were 7.838±0.165 and 11.729±0.346h×μg/ml, respectively. The maximum concentration (Cmax) following IM administration (4.525±0.129 μg/ml) was almost same the mean Cmax after IV administration (4.635±0.270μg/ml). Time to reach maximum concentration (Tmax) after IV and IM administration were 0.1h and 0.5h, respectively. Cefquinome sulphate had a relatively shorter half-life (1.19±0.14h) after IV dose administration. The shorter half-life depicts a rapid elimination. Total recovery after each administration was greater than 75%. The mean resident time after IM and IV administration was found to be 1.528±0.09h and 1.988±0.0311h, respectively. The bioavailability after IM administration was 66.84±2.05%. This study indicated that Cefquinome sulphate has favorable pharmacokinetics following both administrations in healthy layer birds which can help to form optimum dosage regimes thus ultimately leading to its use for eradication of various systemic and local infections. ©2019 PVJ. All rights reserved

接收日期：修订日期：接受日期：在线发布日期：2019年2月19日2019年4月02日2019年04月14日2019年06月26日头孢喹酮被批准用于治疗多种动物的许多细菌感染。本研究旨在评估头孢醌在蛋鸡肌肉内（IM）和静脉内（IV）给药后的药代动力学，以进行安全性评估。12只健康的蛋鸡被随机分为两组。每组通过肌肉注射和静脉注射给药5mg/kg b.w.头孢喹酮。建立了高效液相色谱-紫外检测（HPLC-UV）法测定蛋鸡血浆中头孢醌的含量，回收率>80%。检测限和定量限分别为0.02和0.05μg/ml。药代动力学数据显示，IM和IV给药后的平均曲线下面积（AUC）分别为7.838±0.165和11.729±0.346h×μg/ml。IM给药后最大浓度（Cmax）（4.525±0.129μg/ml）与IV给药后平均Cmax（4.635±0.270μg/ml）几乎相同。IV和IM给药达到最大浓度（Tmax）的时间分别为0.1h和0.5h。静脉给药后，硫酸头孢醌的半衰期相对较短（1.19±0.14小时）。半衰期较短，说明迅速消除。每次给药后的总回收率大于75%。IM和IV给药后的平均停留时间分别为1.528±0.09h和1.988±0.0311h。IM给药后的生物利用度为66.84±2.05%。本研究表明，硫酸头孢醌在健康蛋鸡中两次给药后具有良好的药代动力学，这有助于形成最佳剂量方案，从而最终用于根除各种全身和局部感染。©2019 PVJ。保留所有权利

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引用次数: 2

Antibacterial Activity of Recombinant Porcine β-Defensin 2 重组猪β-防御素2的抗菌活性

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2019-07-01 DOI: 10.29261/pakvetj/2019.030

K. Fan

Received: Revised: Accepted: Published online: September 03, 2018 December 03, 2018 December 12, 2018 March 07, 2019 To express and purify the recombinant porcine β-defensin 2 (rpBD-2) using yeast Pichia Pastoris expression system and in vitro characterization of it’s antibacterial activity. rpBD-2 was expressed and then purified using methylotrophic yeast Pichia Pastoris expression system. To assess its antimicrobial activity against Escherichia coli, Salmonella paratyphi A, Pseudomonas aeruginosa, Staphylococcus epidermidis, Staphylococcus aureus, Bacillus pumilus, and Bacillus subtilis, liquid growth inhibition assay was used. The antibacterial activity was further kinetically evaluated against representative bacterial strains E. coli and S. aureus. The hemolysis activity of rpBD-2 was also checked against porcine erythrocytes. rpBD2 was expressed and purified successfully using yeast Pichia Pastoris expression system. The yield of final product was 9 mg per liter of the harvested supernatant. rpBD-2 possessed antimicrobial activity against E. coli, S. aureus, S. epidermidis, B. subtilis, and B. pumilus at 33.3-66.6 μM and killed all E. coli and S. aureus within 30 min. Negligible hemolytic activity was observed against porcine erythrocytes. We have successfully developed rpBD-2 as an effective antibacterial peptide against both gram-positive and negative bacteria. rpBD-2 will be a potential novel therapeutic agent against bacterial infection. ©2018 PVJ. All rights reserved

接收：修订：接受：在线发布：2018年9月3日2018年12月3日2019年12月12日2019年3月7日使用酵母毕赤酵母表达系统表达和纯化重组猪β-防御素2（rpBD-2），并对其抗菌活性进行体外表征。表达rpBD-2，然后使用甲基营养酵母毕赤酵母表达系统进行纯化。为了评估其对大肠杆菌、甲型副伤寒沙门氏菌、铜绿假单胞菌、表皮葡萄球菌、金黄色葡萄球菌、短小芽孢杆菌和枯草芽孢杆菌的抗菌活性，使用液体生长抑制试验。对代表性菌株大肠杆菌和金黄色葡萄球菌的抗菌活性进行了进一步的动力学评价。还检测了rpBD-2对猪红细胞的溶血活性。利用毕赤酵母表达系统成功表达并纯化了rpBD2。最终产物的产率为每升收获的上清液9mg。rpBD-2对大肠杆菌、金黄色葡萄球菌、表皮葡萄球菌、枯草芽孢杆菌和短小芽孢杆菌具有33.3-66.6μM的抗菌活性，并在30分钟内杀死所有大肠杆菌和金黄色葡萄杆菌。对猪红细胞的溶血活性可忽略不计。我们已经成功开发出rpBD-2，作为一种有效的抗菌肽，可以对抗革兰氏阳性菌和阴性菌。rpBD-2将是一种潜在的新型细菌感染治疗剂。©2018 PVJ。保留所有权利

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引用次数: 3

Protective Effect of Probiotics in Combination with Vaccination on Antibody Response, Biochemical and Hematological Indices in Broilers 益生菌联合疫苗接种对肉仔鸡抗体应答、生化和血液学指标的保护作用

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2019-07-01 DOI: 10.29261/pakvetj/2019.023

Noreen Sarwar

Received: Revised: Accepted: Published online: December 13, 2018 December 20, 2018 December 26, 2018 January 28, 2019 In this study, the effect of multi strain probiotics was studied on antibody response, blood chemistry and hematological parameters in combination with vaccination. A total of sixty day-old broiler chicks were equally divided into four groups: Control, Probiotic, Vaccine and Probiotic + Vaccine. Probiotics were offered at 2g/500ml of water for 5 days/week up to 6 weeks. NDV vaccine was given by eye drops at 0.025ml/bird at day 7. At 21 day NDV Lasota vaccine was offered in drinking distil water at 0.50ml /bird. IBDV vaccine was administered by drinking water at 10, 22 and 30 day of experiment. Blood samples were collected at 15, 30 and 45 days to measure antibody response, blood chemistry and hematological parameters. Antibody titer against NDV vaccine and IBD vaccine showed decline in control and probiotic groups. In case of NDV vaccine, there was no significant difference in Vaccine group (GMT=log2 ) and Probiotic + Vaccine group (GMT=log2 ) at day 45 of experiment. For IBDV vaccine, there was significant difference at 45 day in ELISA titer of vaccine group (2233) and Probiotic+Vaccine group (4206). Cholesterol and ALT showed significant decrease in PV group at 45 day. Significant increase in WBC count was observed in PV group. Level of significance was P≤0.05. Probiotics when administered along with vaccine showed improvement in antibody titer but no harmful increase or decrease in blood chemistry and hematological parameters was observed. ©2018 PVJ. All rights reserved

2018年12月13日2018年12月20日2018年12月26日2019年1月28日本研究研究了多菌株益生菌联合疫苗接种对抗体应答、血液化学和血液学参数的影响。将60日龄肉鸡随机分为对照组、益生菌组、疫苗组和益生菌+疫苗组。益生菌以2g/500ml水的剂量提供，持续5天/周，直至6周。第7天，以0.025ml/只滴眼液方式接种新城疫疫苗。第21天时，用0.50ml /只的蒸馏水饮用NDV Lasota疫苗。分别于试验第10、22、30天饮水接种IBDV疫苗。分别于第15、30和45天采集血样，测定抗体反应、血液化学和血液学参数。对照组和益生菌组抗新城疫疫苗和IBD疫苗的抗体滴度均下降。在接种NDV疫苗的情况下，疫苗组(GMT=log2)和益生菌+疫苗组(GMT=log2)在试验第45天无显著差异。对于IBDV疫苗，45 d时疫苗组(2233)和益生菌+疫苗组(4206)的ELISA滴度差异有统计学意义。第45天，PV组胆固醇和ALT显著降低。PV组WBC计数明显升高。P≤0.05。与疫苗一起使用益生菌可改善抗体滴度，但未观察到血液化学和血液学参数的有害增加或减少。©2018 PVJ。版权所有

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引用次数: 8

Bacteriological, Metabolic and Immunological Evaluation of European sea Bass Reared in Ponds with Heated Water under a Natural Vibriosis-Like Outbreak 天然弧菌病爆发后，温水池饲养欧洲黑鲈的细菌学、代谢和免疫学评价

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2019-07-01 DOI: 10.29261/pakvetj/2019.082

Djamal Mokrani

Received: Revised: Accepted: Published online: July 04, 2017 November 06, 2017 January 24, 2018 June 19, 2019 Fish culture in ponds with heated water offers better fish growth compared to farms in natural sea water temperature, but the increase in temperature favors the development of bacteria and fish disease. In this study, in European sea bass (Dicentrarchus labrax) specimens reared in ponds with heated water from a power plant showing symptoms of a vibriosis-like disease we aimed to characterize the bacterial community as well as some serum metabolic and immune parameters. Bacteriological analysis revealed an important contamination with fecal coliforms, Pseudomonas aeruginosa, Vibrio alginolyticus, yeasts and molds whilst fish tissues from both healthy and diseased specimens were positive for total aerobic flora and several potential pathogenic bacteria such as V. alginolyticus, Aeromonas hydrophila, Pasteurella multocida, P. aeruginosa, Proteus vulgaris and P. mirabilis. Additionally, Grimontia hollisae was only present in diseased fish tissues. Serum metabolic parameters such as aspartate aminotransferase, bile acids, creatine kinase, uric acid, glucose, globulin and potassium were generally increased in diseased fish though only the levels of phosphorous reached a significant level compared to the healthy specimens. Regarding fish innate immunity, our results showed no statistical differences between healthy and diseased fish on peroxidase, protease or antiprotease activity while the bactericidal activity against V. anguillarum was significantly decreased in diseased fish. Lastly, the gene expression of il1b, c3, dic and mpx was down-regulated in the blood of diseased fish while ighm was up-regulated. ©2018 PVJ. All rights reserved

接收日期：修订日期：接受日期：在线发布日期：2017年7月4日2017年11月6日2018年1月24日2019年6月19日与自然海水温度下的养殖场相比，在温水池塘中进行鱼类养殖可以更好地生长，但温度的升高有利于细菌和鱼类疾病的发展。在这项研究中，在用发电厂的热水在池塘中饲养的欧洲鲈鱼（Dicentrarchus labrax）标本中，我们旨在表征细菌群落以及一些血清代谢和免疫参数，这些标本显示出弧菌病样疾病的症状。细菌学分析显示，粪便大肠菌群、铜绿假单胞菌、溶藻弧菌、酵母和霉菌受到了重要污染，而健康和患病标本的鱼类组织中的总需氧菌群和几种潜在致病菌均呈阳性，如溶藻弧菌，嗜水气单胞菌，多杀性巴氏杆菌，绿脓杆菌，普通变形杆菌和奇异变形杆菌。此外，hollisae Grimontia只存在于患病的鱼类组织中。患病鱼类的血清代谢参数，如天冬氨酸氨基转移酶、胆汁酸、肌酸激酶、尿酸、葡萄糖、球蛋白和钾通常会增加，尽管与健康样本相比，只有磷水平达到显著水平。关于鱼类的先天免疫，我们的结果显示，健康鱼类和患病鱼类在过氧化物酶、蛋白酶或抗蛋白酶活性方面没有统计学差异，而患病鱼类对鳗弧菌的杀菌活性显著降低。最后，病鱼血液中il1b、c3、dic和mpx的基因表达下调，而ighm的基因表达上调。©2018 PVJ。保留所有权利

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引用次数: 1