Pakistan Veterinary Journal最新文献

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In vitro Experiments Revealed the Anthelmintic Potential of Herbal Complex against Haemonchus contortus 体外实验显示中药复合物对弯血螨有驱虫作用

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2020-04-01 DOI: 10.29261/pakvetj/2019.128

M. Zaman

Received: Revised: Accepted: Published online: October 8, 2019 December 9, 2019 December 24, 2019 December 31, 2019 In an era of drug resistance, new medicinal plants are being evaluated for control of parasites. In the present study, the anthelmintic activity of the extract of herbal complex (HC) composed of four herbs (Origanum vulgare, Cinnamomum verum, Rosmarinus officinalis and Capsicum annuum) was assessed against eggs and adult Haemonchus (H.) contortus. Egg hatch test (EHT) and adult motility assay (AMA) was performed to evaluate the anthelmintic potential of HC. The reference drug used in EHT was oxfendazole and for AMA two drugs i.e. oxfendazole (2.265 mg ml) and levamisole (3mg ml) were used. In EHT, LC50 values of HC and oxfendazole were 498 and 1.6 ppm, respectively. In AMA, 100% mortality of H. contortus was observed after 6 hr of treatment with HC (100 mg ml) where as two positive control groups could not kill all worms after this exposure time. These results indicated the anthelmintic potential of HC. ©2019 PVJ. All rights reserved

收到:修订:接受:在线发布:2019年10月8日2019年12月9日2019年12月24日2019年12月31日在耐药性时代，新的药用植物正在被评估用于控制寄生虫。本文研究了由四种草本植物组成的中药复合物(HC)提取物(Origanum vulgare、Cinnamomum verum、Rosmarinus officinalis和Capsicum annuum)对弯血螨(Haemonchus contortus)卵和成虫的驱虫活性。采用卵孵化试验(EHT)和成虫运动试验(AMA)评价HC的驱虫潜能。EHT的对照药物为奥芬达唑，AMA的对照药物为奥芬达唑(2.265 mg ml)和左旋咪唑(3mg ml)。在EHT中，HC和奥芬达唑的LC50值分别为498和1.6 ppm。在AMA中，HC (100 mg ml)处理6小时后，观察到弯曲螺旋体的死亡率为100%，而两个阳性对照组在此暴露时间后不能杀死所有蠕虫。这些结果表明了HC的驱虫潜力。©2019 PVJ。版权所有

引用次数: 8

Molecular Characterization of Extensively Drug-resistant Pasteurella multocida Isolated from Apparently Healthy and Diseased Chickens in Egypt 埃及表面健康鸡和病鸡中广泛耐药多杀性巴氏杆菌的分子特征

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.020

Radwa A Elalamy

引用次数: 8

Morphofunctional Assessment of the Glycyrrhizinic Acid Effect on Myocardium of Rats under Adrenaline Loading 甘草酸对肾上腺素负荷大鼠心肌的形态功能影响

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.024

G. Bazekin

引用次数: 2

Effect of Ethanol Extract and Fractions of Physalis Calyx Seu Fructus on Inflammation and Mycoplasma Gallisepticum Physalis Calyx Seu fruct乙醇提取物及提取物对炎症及鸡败支原体的影响

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.036

Yanyan Yi

引用次数: 5

Isolation and Genetic Characterization of Staphylococcus haemolyticus from Cats 猫源溶血葡萄球菌的分离及遗传特性分析

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.033

K. Bierowiec

Received: Revised: Accepted: Published online: November 12, 2019 January 03, 2020 January 15, 2020 April 07, 2020 Staphylococcus haemolyticus is widespread in humans and animals. The aim of this study was to characterize S. haemolyticus isolates from sick and healthy cats according to their antibiotic properties and biofilm formation. A total of 80 S. haemolyticus isolates from 36 healthy and 20 sick cats were investigated. All the isolates from the sick as well as from the healthy animals were multidrug resistant at the genetic level, whereas 82.5% showed phenotypic resistance. Almost all the isolates were methicillin-resistant S. haemolyticus (MRSH) at the genotypic and phenotypic levels of 98.75% and 96.25%, respectively. Moreover, the most frequently observed phenotypic resistances were those for erythromycin (87.5%) and penicillin (93.75%), and the isolates harboured genes involved in resistance to penicillin – blaZ (97.5%), aminoglycosides – aac(6’)Ie-aph(2”)Ia (92.5%), macrolide-lincosamide-streptogramin – ermB (98.75%) and ermC (72.5%), and tetracyclines – tet(K) (98.75%) and tet(M) (100%). The percentage of biofilmpositive strains in healthy and sick cats amounted to 92.68% and 97.44% for the microtiter plate test (P=0.4962) and 71.17% and 56.41% for the Congo red agar assay (P=0.5164), respectively. Statistically significant associations were observed when the owner’s occupation was connected with healthcare, both human or veterinary, there was previous hospitalization of the owner, or cats were kept with other animals in the household. MRSH strains isolated from companion animals were frequently multidrug resistant, although they remained susceptible to antibiotics used in mainly human medicine. ©2020 PVJ. All rights reserved

2019年11月12日2020年1月03日2020年1月15日2020年4月07日溶血葡萄球菌广泛存在于人类和动物中。本研究的目的是根据其抗生素特性和生物膜的形成来表征从病猫和健康猫分离的溶血链球菌。从36只健康猫和20只病猫中分离出80株溶血链球菌。从患病动物和健康动物分离的菌株在遗传水平上均为多重耐药菌株，其中82.5%为表型耐药菌株。几乎所有分离株均为耐甲氧西林溶血链球菌(MRSH)，基因型和表型水平分别为98.75%和96.25%。此外，最常见的表型耐药是红霉素(87.5%)和青霉素(93.75%)，分离株含有青霉素- blaZ(97.5%)，氨基糖苷- aac(6′)Ie-aph(2′)Ia(92.5%)，大环内酯-lincosamide-链状gramin - ermB(98.75%)和ermC(72.5%)，四环素- tet(K)(98.75%)和tet(M)(100%)耐药基因。健康猫和病猫生物膜阳性检出率分别为92.68%和97.44% (P=0.4962)，刚果红琼脂试验检出率分别为71.17%和56.41% (P=0.5164)。当主人的职业与人类或兽医保健有关、主人以前曾住院治疗或猫与家中其他动物一起饲养时，观察到统计上显著的关联。从伴侣动物中分离出的MRSH菌株经常具有多重耐药性，尽管它们仍然对主要用于人类药物的抗生素敏感。©2020 PVJ。版权所有

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引用次数: 3

Serological Study for the Detection of Antibodies against leptospira in Goats 山羊钩端螺旋体抗体检测的血清学研究

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.021

M. U. Aziz

引用次数: 2

Gene Targeted Sequencing Analysis of Mycoplasma gallisepticum Strains in Poultry Flocks from Middle East and South Asia 中东和南亚地区鸡脓毒支原体基因靶向测序分析

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.023

V. Felice

The aim of this study was to attempt the molecular characterization of Mycoplasma gallisepticum (MG) strains detected in chicken flocks from United Arab Emirates (UAE), Saudi Arabia (SAU) and Sri Lanka (SL). Between 2012 and 2015, 55 samples from one layer flock from UAE, three breeder flocks from SAU and one broiler flock from SL were screened for MG using a pr- mgc 2 PCR; the positive samples were characterized by the gene-targeted sequencing (GTS) analysis. BLAST comparisons, followed by phylogenetic analysis, was carried out to compare the obtained sequences against published vaccine and field strain sequences. Fifty of the fifty-five (91%) examined samples, were MG positive by pr- mgc 2 PCR. The sequences detected in the samples from ts-11 vaccinated breeders from SAU showed 100% identity with the ts-11 vaccine sequences. The sequences detected in the samples from layers in UAE, were attributed to field strains, clustering together with Israeli MG strains. The sequences obtained from the samples from Sri Lanka broilers clustered separately from USA, Israeli, Australian, vaccine and reference strains. A potential route of MG field infection to the examined flocks may have been through the movement of birds, either directly (flock-to-flock transmission) or indirectly (through wild bird transmission). To our knowledge, this study reports the first molecular characterization by gene-targeted sequencing (GTS) analysis of MG strains from commercial poultry in SAU, UAE and SL.

本研究旨在对在阿拉伯联合酋长国(UAE)、沙特阿拉伯(SAU)和斯里兰卡(SL)鸡群中检测到的鸡败菌支原体(MG)进行分子鉴定。2012 - 2015年，从阿联酋1只蛋鸡、SAU 3只种鸡和SL 1只肉鸡中选取55份样本，采用pr- mgg2 PCR技术对MG进行了筛选;阳性样品经基因靶向测序(GTS)分析鉴定。BLAST比对，然后进行系统发育分析，将获得的序列与已发表的疫苗和野外菌株序列进行比较。55份检测样本中有50份(91%)经pr- mgg2 PCR检测为MG阳性。从SAU接种ts-11疫苗的育种者样本中检测到的序列与ts-11疫苗序列的一致性为100%。在阿联酋各层样品中检测到的序列属于野外菌株，与以色列MG菌株聚集在一起。从斯里兰卡肉鸡样本中获得的序列分别与美国、以色列、澳大利亚、疫苗和参考菌株聚集在一起。MG野外感染被检查禽群的潜在途径可能是通过鸟类的运动，直接(群对群传播)或间接(通过野鸟传播)。据我们所知，本研究首次报道了通过基因靶向测序(GTS)分析SAU、UAE和SL商品家禽MG菌株的分子特征。

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引用次数: 0

Diversified Epidemiological Pattern and Antibiogram of mecA Gene in Staphylococcus aureus Isolates of Pets, Pet Owners and Environment 宠物、宠物主人及环境金黄色葡萄球菌mecA基因的流行病学特征及抗生素谱分析

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.039

M. Shoaib

引用次数: 16

Serum Anti-Mullerian Hormone Profile from 10 Days of Age to Puberty and Its Relationship with Serum Testosterone and Estradiol Concentrations in Beetal Goat Kids 山羊10日龄至青春期血清抗苗勒管激素水平及其与血清睾酮和雌二醇浓度的关系

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.041

M. Ashraf

Received: Revised: Accepted: Published online: December 4, 2019 March 09, 2020 March 18, 2020 May 04, 2020 The current study aimed to define the serum profile of anti-mullerian hormone (AMH), testosterone and estradiol (E2) concentrations in Beetal goat kids from 10 days of age to puberty (175±12.6 days). The study was conducted from July 2018 to January 2019. Blood samples were collected from male kids (n=26) at 10 days interval from 10 days of age to puberty for measurement of AMH, testosterone and E2 concentrations. The body weight of all kids was measured at 10 days of age and then on monthly basis till puberty. The results showed that the mean body weight at 10 d after birth was 7.04±0.86 kg, and significantly increased till the onset of puberty (36.4±4.49 kg). The minimal plasma AMH concentration was seen at 10 d, gradually increased to its peak level (P<0.05) at 40 d and 100 d and then declined steadily till puberty. The serum testosterone level increased non-significantly from day 10 to day 50 and then gradually increased to its peak level at 170 and 180d (P<0.05). The serum E2 concentration was low at 10 days of age (5.76±0.78 pg/ml), increased to its peak level at 60d (74.3±6.26 pg/ml; P<0.05 compared with 10d), then declined till puberty. The serum AMH at early pre-pubertal age (40, 50, 70 and 80d) was positively correlated with pubertal age around 180d (r=0.99, 0.95, 0.99 and 0.94), whereas testosterone concentration was negatively correlated with serum AMH level at 60d, while positively correlated at 150 and 180d. In conclusion, goat kids exhibit a characteristic serum AMH profile along with testosterone and E2 during pre-pubertal life, such that serum AMH at an early pre-pubertal age could be used as a biomarker for predicting initiation of sexual behavior and onset of puberty. ©2020 PVJ. All rights reserved

本研究旨在确定10日龄至青春期(175±12.6天)Beetal山羊儿童的血清抗苗勒管激素(AMH)、睾酮和雌二醇(E2)浓度。该研究于2018年7月至2019年1月进行。从10日龄至青春期，每隔10天采集26例男童的血样，测定AMH、睾酮和E2浓度。所有儿童的体重在10天大时测量，然后每月测量一次，直到青春期。结果表明，出生后10 d的平均体重为7.04±0.86 kg，到进入青春期后体重显著增加(36.4±4.49 kg)。血浆AMH浓度在10 d时最低，在40 d和100 d时逐渐升高至峰值(P<0.05)，然后稳步下降直至青春期。血清睾酮水平从第10天到第50天无显著升高，在第170天和第180天逐渐升高至最高水平(P<0.05)。血清E2浓度在10日龄时较低(5.76±0.78 pg/ml)， 60d时达到峰值(74.3±6.26 pg/ml);P<0.05)，随后下降至青春期。青春期前早期(40、50、70、80d)血清AMH水平与青春期年龄在180d左右呈显著正相关(r=0.99、0.95、0.99、0.94)，而睾酮浓度与60d时血清AMH水平呈显著负相关，与150、180d时呈显著正相关。综上所述，山羊在青春期前表现出典型的血清AMH特征以及睾酮和E2，因此，青春期前早期的血清AMH可以作为预测性行为开始和青春期开始的生物标志物。©2020 PVJ。版权所有

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引用次数: 0

Molecular Screening of Resistant and Virulent Genes in Salmonella Enteritidis and Salmonella Typhimurium from Poultry in Khyber Pakhtunkhwa 开伯尔-普赫图赫瓦省家禽肠炎沙门氏菌和鼠伤寒沙门氏菌耐药和毒力基因的分子筛选

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.044

Yasin Ahmad

引用次数: 3

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