Pub Date : 2017-07-25DOI: 10.25258/ijpcr.v9i7.8785
T. Padmini, L. Satyanarayana
A validate rapid, economical and sensitive visible spectrophotometric method has been developed for quantitative determination of amprenavir in bulk drug and formulation samples. This method is validated for irinotecan with chromogenic reagent 3-methyl benzothiazolinone hydrazine (MBTH). The calibration curve was linear over Beer’s concentration range of 25-350 µg/ml. The relative standard deviation is less than 1% and average recovery is above 99.80%. Efficient visible spectrophotometric detection at the respective absorption maxima enabled determination with no interference from tablet excipients .The proposed method is fast, sensitive, precise, accurate and efficient and can be used for analysis in quality control laboratories.
{"title":"Spectrophotometric Determination of Amprenavir by ComplexFormation in Bulk Drug and Formulation Samples","authors":"T. Padmini, L. Satyanarayana","doi":"10.25258/ijpcr.v9i7.8785","DOIUrl":"https://doi.org/10.25258/ijpcr.v9i7.8785","url":null,"abstract":"A validate rapid, economical and sensitive visible spectrophotometric method has been developed for quantitative determination of amprenavir in bulk drug and formulation samples. This method is validated for irinotecan with chromogenic reagent 3-methyl benzothiazolinone hydrazine (MBTH). The calibration curve was linear over Beer’s\u0000concentration range of 25-350 µg/ml. The relative standard deviation is less than 1% and average recovery is above 99.80%. Efficient visible spectrophotometric detection at the respective absorption maxima enabled determination with no interference from tablet excipients .The proposed method is fast, sensitive, precise, accurate and efficient and can be used for analysis in quality control laboratories.","PeriodicalId":19889,"journal":{"name":"Pharmaceutical and Clinical Research","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86438226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-07-25DOI: 10.25258/IJPCR.V9I7.8788
Hussain Mobarak, D. Biswajit, Chakraborty Jashabir
Aim: The present research is focused on development and optimization of colon specific, fast disintegrating Capecitabine tablet for the treatment of Colon cancer. Methods: Colon targeted core tablet of Capecitabine was prepared by using CCS (Croscarmellose sodium) as a super disintegrating agent by direct compression method and coating was done over the core tablets by using pectin in different ratios by compression coating method. The colon targeted coating was done on the compression coated tablets by using ES100 and CAP (Cellulose acetate phthalate) in different ratios by dip coating method. In vitro swelling studies were carried out at different pH (1.2, 6.8 and 7.4). The Design Expert software (v.10) was used to optimise the best formulation and an in vitro cumulative percentage of drug release in different dissolution media (pH 1.2, 6.8 and 7.4) with respect to the time interval (2hr, 7hr and 9hr) as dependable variable. Results: Optimized formulation of Capecitabine tablet shows satisfactory result with respect to all pre and post compression test parameters and it was significantly stable during stability studies conducted for 30and 60 days. Conclusion: From the above research it was found that, the optimised formulation of less half-life period anticancer drug Capecitabine can be properly targeted to colon area with the help of pectin and eudragit S 100.
{"title":"Formulation, Optimization and Evaluation of Capecitabine Tablet for Colon Specific Drug Delivery System","authors":"Hussain Mobarak, D. Biswajit, Chakraborty Jashabir","doi":"10.25258/IJPCR.V9I7.8788","DOIUrl":"https://doi.org/10.25258/IJPCR.V9I7.8788","url":null,"abstract":"Aim: The present research is focused on development and optimization of colon specific, fast disintegrating Capecitabine tablet for the treatment of Colon cancer. Methods: Colon targeted core tablet of Capecitabine was prepared by using CCS (Croscarmellose sodium) as a super disintegrating agent by direct compression method and coating was done over the core tablets by using pectin in different ratios by compression coating method. The colon targeted coating was done on the compression coated tablets by using ES100 and CAP (Cellulose acetate phthalate) in different ratios by dip coating method. In vitro swelling studies were carried out at different pH (1.2, 6.8 and 7.4). The Design Expert software (v.10) was used to optimise the best formulation and an in vitro cumulative percentage of drug release in different dissolution media (pH 1.2,\u00006.8 and 7.4) with respect to the time interval (2hr, 7hr and 9hr) as dependable variable. Results: Optimized formulation of Capecitabine tablet shows satisfactory result with respect to all pre and post compression test parameters and it was significantly stable during stability studies conducted for 30and 60 days. Conclusion: From the above research it was found that, the optimised formulation of less half-life period anticancer drug Capecitabine can be properly targeted to colon area with the help of pectin and eudragit S 100.","PeriodicalId":19889,"journal":{"name":"Pharmaceutical and Clinical Research","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79159680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-07-25DOI: 10.25258/IJPCR.V9I7.8783
K. Ravindrachary, M. Ramesh, T. Parthasarathy
The poly (ADP-ribose) polymerase-1 (PARP-1) is an abundant nuclear protein involved in DNA repair and programmed cell death. Substituted 5(H) phenanthradin-6-one analogs were found to be potent PARP-1 inhibitors. Semiempirical methods were used to estimate various physicochemical parameters. The hydration energy (HE), ionization potential (IP), electrophilic index (ω) and partition coefficient ( LogP ) were resulted as independent variables for inhibitory activity of the analogs. The overall increase of HE, IP, and EI and overall decrease of LogP enhance the efficacy of inhibitory nature of these analogs to PARP-1. Docking studies of 5(H) phenanthradin-6-one analogs with PARP-1 were also performed in support of the findings of QSAR studies. Analysis of results of both QSAR and docking studies suggested that remarkable inhibitory activity is exhibited by molecules 9b, 10b1 and 10b2. The hydrogen bond interactions along with hydrophobic and electrostatic interactions are mapped to confirm their potencies.
{"title":"Computational Study of Substituted 5[H] - Phenanthradin-6-Ones as Poly (ADP-Ribose) Polymerase-1 (PARP-1) Inhibitors by Analog and Structure Based Methods","authors":"K. Ravindrachary, M. Ramesh, T. Parthasarathy","doi":"10.25258/IJPCR.V9I7.8783","DOIUrl":"https://doi.org/10.25258/IJPCR.V9I7.8783","url":null,"abstract":"The poly (ADP-ribose) polymerase-1 (PARP-1) is an abundant nuclear protein involved in DNA repair and programmed\u0000cell death. Substituted 5(H) phenanthradin-6-one analogs were found to be potent PARP-1 inhibitors. Semiempirical\u0000methods were used to estimate various physicochemical parameters. The hydration energy (HE), ionization potential (IP),\u0000electrophilic index (ω) and partition coefficient ( LogP ) were resulted as independent variables for inhibitory activity of\u0000the analogs. The overall increase of HE, IP, and EI and overall decrease of LogP enhance the efficacy of inhibitory nature\u0000of these analogs to PARP-1. Docking studies of 5(H) phenanthradin-6-one analogs with PARP-1 were also performed in\u0000support of the findings of QSAR studies. Analysis of results of both QSAR and docking studies suggested that remarkable\u0000inhibitory activity is exhibited by molecules 9b, 10b1 and 10b2. The hydrogen bond interactions along with hydrophobic\u0000and electrostatic interactions are mapped to confirm their potencies.","PeriodicalId":19889,"journal":{"name":"Pharmaceutical and Clinical Research","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75913133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-07-25DOI: 10.25258/IJPCR.V9I7.8784
G. Kalyani, S. Bethi, K. V. Sastry, V. Kuchana
In the present study, we have designed and synthesized a series of novel cinnoline fused Mannich bases by the condensation reaction of 4-methyl-3-acetyl cinnoline with different secondary aromatic and aliphatic amines with and the structures of compounds were characterized by H1-NMR, IR and Mass spectral analysis. The biological potentials of the newly synthesized compounds are evaluated for their antibacterial activity against Staphylococcus aureus (Gram positive), and Eeshricia coli (Gram negative) bacteria, in vivo analgesic and anti-inflammatory activities. Compounds 4 and 3, which are having larger hydrophobic amino substitutions resulted in relatively higher antibacterial against S. aureus and E. coli when compared to streptomycin. Similarly, compound 4 reported higher analgesic activity when compared to diclofenac at 120 mins and 180 mins. From anti-inflammatory evaluations, dose level of 50 mg/kg of test compounds reported significantly higher activity when compared to dose level of 20 mg/kg. Moreover, compound 4 (50 mg/kg) resulted in similar antiinflammatory activity when compared with celecoxib (20 mg/kg).
{"title":"Synthesis of Novel Cinnoline Fused Mannich Bases: Pharmacological Evaluation of Antibacterial, Analgesic and Anti-Inflammatory Activities","authors":"G. Kalyani, S. Bethi, K. V. Sastry, V. Kuchana","doi":"10.25258/IJPCR.V9I7.8784","DOIUrl":"https://doi.org/10.25258/IJPCR.V9I7.8784","url":null,"abstract":"In the present study, we have designed and synthesized a series of novel cinnoline fused Mannich bases by the condensation\u0000reaction of 4-methyl-3-acetyl cinnoline with different secondary aromatic and aliphatic amines with and the structures of\u0000compounds were characterized by H1-NMR, IR and Mass spectral analysis. The biological potentials of the newly\u0000synthesized compounds are evaluated for their antibacterial activity against Staphylococcus aureus (Gram positive), and\u0000Eeshricia coli (Gram negative) bacteria, in vivo analgesic and anti-inflammatory activities. Compounds 4 and 3, which are\u0000having larger hydrophobic amino substitutions resulted in relatively higher antibacterial against S. aureus and E. coli when\u0000compared to streptomycin. Similarly, compound 4 reported higher analgesic activity when compared to diclofenac at 120\u0000mins and 180 mins. From anti-inflammatory evaluations, dose level of 50 mg/kg of test compounds reported significantly\u0000higher activity when compared to dose level of 20 mg/kg. Moreover, compound 4 (50 mg/kg) resulted in similar antiinflammatory\u0000activity when compared with celecoxib (20 mg/kg).","PeriodicalId":19889,"journal":{"name":"Pharmaceutical and Clinical Research","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88858808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-07-25DOI: 10.25258/IJPCR.V9I7.8786
Manish Kumar, M. Venkatesh, T. M. Kumar
Colour additives have long been used as a means of enhancing the aesthetic value of foods, beverages, and cosmetics and of identifying drugs and other products. Naturally occurring substances such as turmeric, paprika, saffron, and inorganic mineral pigments have been used for thousands of years. In the middle of the nineteenth century, synthetic organic dyes were developed, creating an economical and extensive array of color additives. Certified color additives are synthetic organic compounds. Most exempt colorants are derived from vegetable or mineral sources and are therefore often called "natural"; they can also be made synthetically, however it requires FDA to hold both types of color additives to the same safety standard. Colour additives exempt from certification (also known as ‘natural Colour additives’) are commonly used in the United States to colour foods, drugs and cosmetics. The US Food and Drug Administration established regulations governing the use of these colour additives, and the labelling of the products that contain them. The safety of these colour additives has been demonstrated by safety testing programs that have yielded a significant amount of toxicology data, and also by a long and well documented history of safe use in the United States and elsewhere. In this study the data supporting the safety of the colour additives that are exempt from certification and approved for use in the USA, and conclude that the safety of these colour additives are well supported by the literature.
{"title":"Colourants and Additives: Existing and Emerging Safety Concerns","authors":"Manish Kumar, M. Venkatesh, T. M. Kumar","doi":"10.25258/IJPCR.V9I7.8786","DOIUrl":"https://doi.org/10.25258/IJPCR.V9I7.8786","url":null,"abstract":"Colour additives have long been used as a means of enhancing the aesthetic value of foods, beverages, and cosmetics and of identifying drugs and other products. Naturally occurring substances such as turmeric, paprika, saffron, and inorganic mineral pigments have been used for thousands of years. In the middle of the nineteenth century, synthetic organic dyes were developed, creating an economical and extensive array of color additives. Certified color additives are synthetic organic compounds. Most exempt colorants are derived from vegetable or mineral sources and are therefore often called \"natural\"; they can also be made synthetically, however it requires FDA to hold both types of color additives to the same safety standard. Colour additives exempt from certification (also known as ‘natural Colour additives’) are commonly used in the United States to colour foods, drugs and cosmetics. The US Food and Drug Administration established regulations governing the use of these colour additives, and the labelling of the products that contain them. The safety of these colour additives has been demonstrated by safety testing programs that have yielded a significant amount of toxicology data, and also by a long and well documented history of safe use in the United States and elsewhere. In this study the data supporting the safety of the colour additives that are exempt from certification and approved for use in the USA, and conclude that the safety of these colour additives are well supported by the literature.","PeriodicalId":19889,"journal":{"name":"Pharmaceutical and Clinical Research","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81081089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-07-25DOI: 10.25258/IJPCR.V9I7.8787
Ajeet Singh, Navneet
The antibacterial and phytochemical investigation of Barleria lupulina Lindl. aerial parts extracts were examined against common respiratory tract pathogens i.e., Streptococcus pneumoniae (MTCC 655), Staphylococcus aureus (MTCC 1144), Pseudomonas aeruginosa (MTCC 2474), Streptococcus pyogens (MTCC 442), Haemophillus influenzae (MTCC 3826). The plant material was extracted with solvents i.e., petroleum ether (PET), acetone (ACE), methanol (MeOH) and aqueous (H2O) with increasing polarity by Soxhlet apparatus and removed the solvent using vacuum evaporator at 30˚C. Antibacterial activity and minimum inhibitory concentration (MICs) were examined by Agar well diffusion two fold serial dilution method respectively. The maximum inhibition zone was found against S. aureus (17.34±0.78 mm) of methanol extract and minimum against S. pyogens (9.44±0.32 mm). MICs were observed for MeOH extract between 3.12 to 12.5 mg/mL against S. pneumoniae and S. pyogens respectively. Phytochemical examination of plant extracts showed the occurrence of alkaloids, saponins, steroids, flavonoids, glycosides, tannins, resins and phenolic compounds. The antimicrobial activity of the crude extracts of plant represents a significant outcome for the treatment of respiratory tract diseases.
{"title":"Antibacterial Potential and Phytochemical Analysis of Barleria lupulina Lindl. (Aerial Parts) Extracts Against Respiratory Tract Pathogens","authors":"Ajeet Singh, Navneet","doi":"10.25258/IJPCR.V9I7.8787","DOIUrl":"https://doi.org/10.25258/IJPCR.V9I7.8787","url":null,"abstract":"The antibacterial and phytochemical investigation of Barleria lupulina Lindl. aerial parts extracts were examined against common respiratory tract pathogens i.e., Streptococcus pneumoniae (MTCC 655), Staphylococcus aureus (MTCC 1144), Pseudomonas aeruginosa (MTCC 2474), Streptococcus pyogens (MTCC 442), Haemophillus influenzae (MTCC 3826). The plant material was extracted with solvents i.e., petroleum ether (PET), acetone (ACE), methanol (MeOH) and aqueous (H2O) with increasing polarity by Soxhlet apparatus and removed the solvent using vacuum evaporator at 30˚C. Antibacterial activity and minimum inhibitory concentration (MICs) were examined by Agar well diffusion two fold serial\u0000dilution method respectively. The maximum inhibition zone was found against S. aureus (17.34±0.78 mm) of methanol extract and minimum against S. pyogens (9.44±0.32 mm). MICs were observed for MeOH extract between 3.12 to 12.5 mg/mL against S. pneumoniae and S. pyogens respectively. Phytochemical examination of plant extracts showed the occurrence of alkaloids, saponins, steroids, flavonoids, glycosides, tannins, resins and phenolic compounds. The antimicrobial activity of the crude extracts of plant represents a significant outcome for the treatment of respiratory tract diseases.","PeriodicalId":19889,"journal":{"name":"Pharmaceutical and Clinical Research","volume":"62 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83417268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-06-25DOI: 10.25258/IJPCR.V9I6.8775
S. Hemalatha, P. Srikanth, G. Sai
In present investigation an attempt has been made to design and develop the Bilayered tablet of Glimepiride and Metformin using Mangifera Indica Gum (MIG) and HPMC as Immediate Release and Sustained Release Layer polymers. Glimepiride and Metformin are oral-hypoglycaemic drugs which lower blood glucose level and have been selected to prepare Bilayered tablets. Glimepiride immediate release layer was prepared using MIG by wet granulation technique and Metformin sustained release layer was prepared using HPMC by dry granulation technique. Prepared Bilayered tablets were evaluated for parameters like thickness, diameter, weight variation, hardness, friability, disintegration and in-vitro release studies. All the prepared tablets were of smooth surface and elegant texture. The weights of the tablets were in the range of 540±0.551 mg. The thicknesses of the tablet were in the range of 4±0.05mm. The drug content uniformity study showed uniform dispersion of drug throughout the formulation in the range of 97.16±0.50%. The hardness was in the range of 4.0±0.5 kg/cm2 and friability is in the range of 0.67±0.06%. The bilayered tablets were also subjected to model fitting analysis to know the order and mechanism of drug release from the formulation by treating the data according to zeroorder, first-order, Higuchi and peppas equations.The bioequivalence studies conducted between prepared and marketed (Glycomate) bilayered tablet showed the similarity factor value of 70.120 for IR layer and 57.689 for SR layer.
{"title":"Formulation and Evaluation of Bilayered Tablets Containing Immediate Release Layer of Glimepiride Complexed with Mangifera indica Gum and Sustained Release Layer Containing Metformin HCL by Using HPMC as Release Retardant","authors":"S. Hemalatha, P. Srikanth, G. Sai","doi":"10.25258/IJPCR.V9I6.8775","DOIUrl":"https://doi.org/10.25258/IJPCR.V9I6.8775","url":null,"abstract":"In present investigation an attempt has been made to design and develop the Bilayered tablet of Glimepiride and Metformin using Mangifera Indica Gum (MIG) and HPMC as Immediate Release and Sustained Release Layer polymers. Glimepiride and Metformin are oral-hypoglycaemic drugs which lower blood glucose level and have been selected to prepare Bilayered tablets. Glimepiride immediate release layer was prepared using MIG by wet granulation technique and Metformin sustained release layer was prepared using HPMC by dry granulation technique. Prepared Bilayered tablets were evaluated for parameters like thickness, diameter, weight variation, hardness, friability, disintegration and in-vitro release studies. All the prepared tablets were of smooth surface and elegant texture. The weights of the tablets were in the range of 540±0.551 mg. The thicknesses of the tablet were in the range of 4±0.05mm. The drug content uniformity study showed uniform dispersion of drug throughout the formulation in the range of 97.16±0.50%. The hardness was in the range of 4.0±0.5 kg/cm2 and friability is in the range of 0.67±0.06%. The bilayered tablets were also subjected to model fitting analysis to know the order and mechanism of drug release from the formulation by treating the data according to zeroorder, first-order, Higuchi and peppas equations.The bioequivalence studies conducted between prepared and marketed (Glycomate) bilayered tablet showed the similarity factor value of 70.120 for IR layer and 57.689 for SR layer.","PeriodicalId":19889,"journal":{"name":"Pharmaceutical and Clinical Research","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86455691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-06-25DOI: 10.25258/IJPCR.V9I6.8774
Zenia Angelina, E. Sulistijono, L. Fitri
Objective: The aim of this study is to prove the relationship of FcγRI (CD64) expression and Procalcitonin value in earlyonset neonatal sepsis to assist in diagnosis of early-onset neonatal sepsis. Method: A descriptive and analytical case control study was conducted in dr. Saiful Anwar General Hospital Malang. There were 40 children divided into two groups: 1). Group of infants with neonatal risk factors who express signs of SIRS and proven by blood culture; 2). Group of infants with neonatal risk factors who showed no sign for SIRS. Both groups were performed examination of FcγRI (CD64) expression with flowcytometry and Procalcitonin value with ELISA. Data were statistically analyzed using normality test (Kolmogorov-Smirnov), chi square test, t test and Pearson correlation. We used SPSS 16 for this analysis. Results: The study showed that the FcγRI (CD64) expression and Procalcitonin value were higher in the infants group with proven early-onset neonatal sepsis (p less than 0.05). There was a significant relationship between FcγRI (CD64) expression and the Procalcitonin value (p=0.036). Conclusions: We conclude that expression of FcγRI (CD64) and Procalcitonin value were higher in the infants group with proven early-onset neonatal sepsis. There is a positive relationship between FcγRI (CD64) expression and Procalcitonin value in early onset neonatal sepsis
目的:本研究旨在证实fc γ - ri (CD64)表达与降钙素原值在新生儿早发性脓毒症中的关系，以辅助对新生儿早发性脓毒症的诊断。方法:采用描述性和分析性病例对照研究。40例患儿分为两组:1)有SIRS体征并经血培养证实的新生儿危险因素组;2)有新生儿危险因素但未出现SIRS症状的婴儿组。两组均采用流式细胞术检测fc - γ - ri (CD64)表达，ELISA检测降钙素原值。数据采用正态性检验(Kolmogorov-Smirnov)、卡方检验、t检验和Pearson相关进行统计学分析。我们使用SPSS 16进行分析。结果:早发新生儿脓毒症患儿组fc γ - ri (CD64)表达及降钙素原值升高(p < 0.05)。fc γ - ri (CD64)表达与降钙素原值有显著相关(p=0.036)。结论:我们认为fc γ - ri (CD64)的表达和降钙素原值在早发性新生儿败血症的婴儿组中较高。早期新生儿败血症中fc γ - ri (CD64)表达与降钙素原呈正相关
{"title":"The Correlation of Fc-gamma Receptor I (CD64) Expression and Procalcitonin in Early Onset Neonatal Sepsis","authors":"Zenia Angelina, E. Sulistijono, L. Fitri","doi":"10.25258/IJPCR.V9I6.8774","DOIUrl":"https://doi.org/10.25258/IJPCR.V9I6.8774","url":null,"abstract":"Objective: The aim of this study is to prove the relationship of FcγRI (CD64) expression and Procalcitonin value in earlyonset neonatal sepsis to assist in diagnosis of early-onset neonatal sepsis. Method: A descriptive and analytical case control study was conducted in dr. Saiful Anwar General Hospital Malang. There were 40 children divided into two groups: 1). Group of infants with neonatal risk factors who express signs of SIRS and proven by blood culture; 2). Group of infants with neonatal risk factors who showed no sign for SIRS. Both groups were performed examination of FcγRI (CD64) expression with flowcytometry and Procalcitonin value with ELISA. Data were statistically analyzed using normality test (Kolmogorov-Smirnov), chi square test, t test and Pearson correlation. We used SPSS 16 for this analysis. Results: The study showed that the FcγRI (CD64) expression and Procalcitonin value were higher in the infants group with proven early-onset neonatal sepsis (p less than 0.05). There was a significant relationship between FcγRI (CD64) expression and the Procalcitonin value (p=0.036). Conclusions: We conclude that expression of FcγRI (CD64) and Procalcitonin value were higher in the infants group with proven early-onset neonatal sepsis. There is a positive relationship between FcγRI (CD64) expression and Procalcitonin value in early onset neonatal sepsis","PeriodicalId":19889,"journal":{"name":"Pharmaceutical and Clinical Research","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84643207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-06-25DOI: 10.25258/IJPCR.V9I6.8771
Techit Thavorasak, Paphanin Kamkaen, Araya Rattanasri, S. Tangvarasittichai
Type 2 diabetes mellitus (T2DM) is a common metabolic disorder and one of the leading causes of morbidity and mortality in both developed and developing countries. Early recognition and intervention will be helpful in reducing the personal and financial cost of the disease. We used the diabetic risk score (DRS) and fasting plasma glucose test (FPGT) for identification the risk of T2DM. A total of 142 female participants were randomly participated in the present study. These participants were identified as 39 (27.5%) high risk (Gr-III) and 71 (50%) very high risk (Gr-IV) for T2DM groups according to the DRS. In addition with 13 (9.2%) and 2 (1.4%) were newly diagnosed as having HT and T2DM. Both HT and T2DM participants were older than the normal participants. BMI and WC were not significantly different in the comparison of T2DM with Non-T2DM and HT with Non-HT patients. The DRS would be practical to use as tool for T2DM risk screening while FPGT was used to identify impaired fasting glucose and T2DM onset. Then, we recommended FPGT for the individuals with high and very high DRS groups.
{"title":"Diabetic Risk Score and Fasting Plasma Glucose Testing in the Screening for Type 2 Diabetes Mellitus Risk","authors":"Techit Thavorasak, Paphanin Kamkaen, Araya Rattanasri, S. Tangvarasittichai","doi":"10.25258/IJPCR.V9I6.8771","DOIUrl":"https://doi.org/10.25258/IJPCR.V9I6.8771","url":null,"abstract":"Type 2 diabetes mellitus (T2DM) is a common metabolic disorder and one of the leading causes of morbidity and mortality in both developed and developing countries. Early recognition and intervention will be helpful in reducing the personal and financial cost of the disease. We used the diabetic risk score (DRS) and fasting plasma glucose test (FPGT) for identification the risk of T2DM. A total of 142 female participants were randomly participated in the present study. These participants were identified as 39 (27.5%) high risk (Gr-III) and 71 (50%) very high risk (Gr-IV) for T2DM groups according to the DRS. In addition with 13 (9.2%) and 2 (1.4%) were newly diagnosed as having HT and T2DM. Both HT and T2DM participants were older than the normal participants. BMI and WC were not significantly different in the comparison of T2DM with Non-T2DM and HT with Non-HT patients. The DRS would be practical to use as tool for T2DM risk screening while FPGT was used to identify impaired fasting glucose and T2DM onset. Then, we recommended FPGT for the individuals with high and very high DRS groups.","PeriodicalId":19889,"journal":{"name":"Pharmaceutical and Clinical Research","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89420150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-06-25DOI: 10.25258/ijpcr.v9i6.8776
Ankita Sharma, D. V. Gowda, N. Gupta, R. Osmani
Peptides are the building blocks which are widely used owing to their biology as well as their chemistry. They provide a vast platform in the area of medicine. Self-assembling peptides are peptide biomaterials which are pacing in the field of diagnosis, therapeutics, tissue regeneration and vaccine. Self-assembling peptides provide an excellent alternative to the conventional methods for the drug delivery and the treatment. In this article, we discuss about the various medical applications of self-assembling peptide as they have excellent biocompatibility and resemblance with the proteins in the biological system. These are constructed and modified using various amino acid sequences depending upon the type of the application for which it is being used
{"title":"Self-Assembling Peptides- Notion and Medical Applications: A Review","authors":"Ankita Sharma, D. V. Gowda, N. Gupta, R. Osmani","doi":"10.25258/ijpcr.v9i6.8776","DOIUrl":"https://doi.org/10.25258/ijpcr.v9i6.8776","url":null,"abstract":"Peptides are the building blocks which are widely used owing to their biology as well as their chemistry. They provide a vast platform in the area of medicine. Self-assembling peptides are peptide biomaterials which are pacing in the field of diagnosis, therapeutics, tissue regeneration and vaccine. Self-assembling peptides provide an excellent alternative to the conventional methods for the drug delivery and the treatment. In this article, we discuss about the various medical applications of self-assembling peptide as they have excellent biocompatibility and resemblance with the proteins in the biological system. These are constructed and modified using various amino acid sequences depending upon the type of the application for which it is being used","PeriodicalId":19889,"journal":{"name":"Pharmaceutical and Clinical Research","volume":"89 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78382814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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