Pub Date : 2017-02-25DOI: 10.25258/IJPCR.V9I2.8297
A. Elaimi, Fenita Shoviantari, T. Erawati, Widji Soeratri
Penetration is an absolute requirement for active ingredient to produce an effect in deep of the skin. First obstacle for active ingredient to penetrate through the skin is stratum corneum. Nanotechnology that has nano size particles, is the answer to make it’s penetrate through the skin. In this research the skin penetration of Coenzyme Q10 was loaded in the lipid nanoparticles that is nanostructure lipid carrier (NLC) was compered with it was dissolved in olive oil. The research result known that Coenzyme Q10 was loaded in NLC has deeper penetration (639.34 ± 17.69 μm) in the mice skin than it was dissolved in olive oil as control (33.00 ± 1.71 μm), its just least penetrated into the skin after 6 hours sample application.
{"title":"Skin Penetration of Coenzyme Q10 in Nanostructure Lipid Carriers Using Olive Oil and Cetyl Palmitate","authors":"A. Elaimi, Fenita Shoviantari, T. Erawati, Widji Soeratri","doi":"10.25258/IJPCR.V9I2.8297","DOIUrl":"https://doi.org/10.25258/IJPCR.V9I2.8297","url":null,"abstract":"Penetration is an absolute requirement for active ingredient to produce an effect in deep of the skin. First obstacle for active ingredient to penetrate through the skin is stratum corneum. Nanotechnology that has nano size particles, is the answer to make it’s penetrate through the skin. In this research the skin penetration of Coenzyme Q10 was loaded in the lipid nanoparticles that is nanostructure lipid carrier (NLC) was compered with it was dissolved in olive oil. The research result known that Coenzyme Q10 was loaded in NLC has deeper penetration (639.34 ± 17.69 μm) in the mice skin than it was dissolved in olive oil as control (33.00 ± 1.71 μm), its just least penetrated into the skin after 6 hours sample application.","PeriodicalId":19889,"journal":{"name":"药学与临床研究","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76691003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-02-25DOI: 10.25258/IJPCR.V9I2.8301
M. A. Khaleel, Amean A. Al-Yaseri
The researchers had conducted this descriptive study aimed at given the nursing students at university of Babylon chance to reflect on their opinions of clinical teaching and evaluation. The study consisted of (136) third and fourth year nursing students from college of nursing/University of Babylon. Data were gathered by using questionnaire form which consisted of four items (21 statements). All items were rated on 3 Point Likert Scale whereas (Always, Sometimes and Never). Data analysis was done through the application of simple descriptive statistical methods such as frequencies and percentages to assess the results of the study. The results of the study indicated that some of the teaching skills of the clinical teacher components are at accepted levels, other like quality of bedside teaching (integrating theory to practice) needs more efforts on the part of the teacher. Also, results showed the responses for the statement clinical teacher provided chances to practice skills (37%) and responses to statement encouraged students to actively participate in the discussions (37%) needs to be looked into future. As well as results showed that the opportunities for practicing nursing skills, (46%) seems to show that less than half of the students are able to achieve these opportunities. The study concluded that the learning environment created by the teacher and his/her characteristics can and does have a profound effect on learning and the challenge for nurse education is to provide learning experience that facilitates the so called knowledge doer. Finally, the study recommended the necessity for improving the quality of the theoretical knowledge and bedside teaching in the clinical area and emphasized on provision for more chances to practice skills
{"title":"Reflections on Quality Clinical Teaching and Evaluation Through the Input of Nursing Students at the University of Babylon in Iraq","authors":"M. A. Khaleel, Amean A. Al-Yaseri","doi":"10.25258/IJPCR.V9I2.8301","DOIUrl":"https://doi.org/10.25258/IJPCR.V9I2.8301","url":null,"abstract":"The researchers had conducted this descriptive study aimed at given the nursing students at university of Babylon chance\u0000to reflect on their opinions of clinical teaching and evaluation. The study consisted of (136) third and fourth year nursing\u0000students from college of nursing/University of Babylon. Data were gathered by using questionnaire form which consisted\u0000of four items (21 statements). All items were rated on 3 Point Likert Scale whereas (Always, Sometimes and Never). Data\u0000analysis was done through the application of simple descriptive statistical methods such as frequencies and percentages to\u0000assess the results of the study. The results of the study indicated that some of the teaching skills of the clinical teacher\u0000components are at accepted levels, other like quality of bedside teaching (integrating theory to practice) needs more efforts\u0000on the part of the teacher. Also, results showed the responses for the statement clinical teacher provided chances to practice\u0000skills (37%) and responses to statement encouraged students to actively participate in the discussions (37%) needs to be\u0000looked into future. As well as results showed that the opportunities for practicing nursing skills, (46%) seems to show that\u0000less than half of the students are able to achieve these opportunities. The study concluded that the learning environment\u0000created by the teacher and his/her characteristics can and does have a profound effect on learning and the challenge for\u0000nurse education is to provide learning experience that facilitates the so called knowledge doer. Finally, the study\u0000recommended the necessity for improving the quality of the theoretical knowledge and bedside teaching in the clinical area\u0000and emphasized on provision for more chances to practice skills","PeriodicalId":19889,"journal":{"name":"药学与临床研究","volume":"93 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83886031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-02-25DOI: 10.25258/IJPCR.V9I2.8293
T. Vidhya, C. Kanniammal, J. Mahendra, G. Valli
Background: Cardiovascular diseases (CVD) are the leading cause of the mortality in urban and rural India and foremost causes of the death worldwide. Hypertension is a major risk condition that plays a pivotal role regarding the complications for those diagnosed with Acute Coronary Syndrome. According to the World Health Organization (2012), hypertension is the number one cause of mortality in the world and it is a major risk factor for cardiac diseases and stroke. Objectives: i) To identify the level of blood pressure (BP) in pre and post test among study and control group of patients with acute coronary syndrome. ii) Determine the effect of cardiac walking on blood pressure among patients with ACS. iii) Associate the selected back ground variables with the level of systolic BP in the post test among study and control group. Methods: A quasi experimental design was adopted for this study. There are 50 Patients diagnosed with ASC admitted for the treatment and attending cardiac OPD were selected by purposive sampling technique of non- probability type. The intervention cardiac walking given for a period of one month to the study group. Findings: The comparison of before and after systolic and diastolic blood pressure (BP) within study group depicted statistically significant difference at **p less than 0.001 but not in control group. The comparison of post systolic and diastolic BP between study and control group inferred that there was statistically significant difference at **p less than 0.001. Conclusion: Cardiac walking is most effective way to stabilize the blood pressure among patients with ACS in order to prevent further complications such as stroke.
{"title":"Effectiveness of Cardiac Walking on Blood Pressure Among Patients with Acute Coronary Syndrome","authors":"T. Vidhya, C. Kanniammal, J. Mahendra, G. Valli","doi":"10.25258/IJPCR.V9I2.8293","DOIUrl":"https://doi.org/10.25258/IJPCR.V9I2.8293","url":null,"abstract":"Background: Cardiovascular diseases (CVD) are the leading cause of the mortality in urban and rural India and foremost\u0000causes of the death worldwide. Hypertension is a major risk condition that plays a pivotal role regarding the complications\u0000for those diagnosed with Acute Coronary Syndrome. According to the World Health Organization (2012), hypertension is\u0000the number one cause of mortality in the world and it is a major risk factor for cardiac diseases and stroke. Objectives: i)\u0000To identify the level of blood pressure (BP) in pre and post test among study and control group of patients with acute\u0000coronary syndrome. ii) Determine the effect of cardiac walking on blood pressure among patients with ACS. iii) Associate\u0000the selected back ground variables with the level of systolic BP in the post test among study and control group. Methods:\u0000A quasi experimental design was adopted for this study. There are 50 Patients diagnosed with ASC admitted for the\u0000treatment and attending cardiac OPD were selected by purposive sampling technique of non- probability type. The\u0000intervention cardiac walking given for a period of one month to the study group. Findings: The comparison of before and\u0000after systolic and diastolic blood pressure (BP) within study group depicted statistically significant difference at **p less than 0.001\u0000but not in control group. The comparison of post systolic and diastolic BP between study and control group inferred that\u0000there was statistically significant difference at **p less than 0.001. Conclusion: Cardiac walking is most effective way to stabilize\u0000the blood pressure among patients with ACS in order to prevent further complications such as stroke.","PeriodicalId":19889,"journal":{"name":"药学与临床研究","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85613576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-02-25DOI: 10.25258/IJPCR.V9I2.8294
A. Puspaningtyas
A new compound of Mefenamic Acid derivate, 4-nitrobenzoyl-mefenamic acid has been synthesized by benzoylation reaction between mefenamic acid and 4-nitrobenzoyl chloride after prediction by in silico study/molecular approach. A derivative of mefenamic acid (4-NO2-benzoyl-mefenamic acid) has been synthesized for increase its activity as candidate of analgesic drug/inhibitor COX-2 (Cyclooxigenase-2). This compound has been purified by Column Chromatography and analyzed using TLC-Densitometry to determine purity with Rf value 0,8. The spot has good purity and then it was identified this structure using H-NMR 400 MHz and FTIR-KBr. The result showed that this compound is 4-nitrobenzoyl-mefenamic acid (4NBMA). 4NBMA gives white yellow color with melting point 198-199C. Finally, 4NBMA was tested analgetic activity by hot plate method and it showed that 4-nitrobenzoyl-mefenamic acid has been higher activity than mefenamic acid. Keyword: 4-nitrobenzoyl-mefenamic acid, analgesic, benzoylation, molecular approach. INTRODUCTION Pain is a multidimentional sensory experience. International Association for the Study of Pain (IASP) defines that pain is a sensory and unpleasant emotional experience associated with tissue damage, both actual and potential. Chronic pain becomes a serious problem if it increases rate of pain and gives chronic prevalence. Pain is one of the most frequently reported symptom occurs in one from six people in the population and it is estimated to occur in 2-40% of adult population. Some studies estimate that the prevalence of chronic pain in Europe is up to 55.2%. In Indonesia, the population of the elderly, reported that 25-50% of them experienced pain. Chronic pain causes increasing health care costs. The research in the United States showed that the cost yearly for chronic pain is estimated around 100 billion dollars. Mefenamic acid is a drug in the market as NSAIDs (Non-steroidal AntiInflammatory Drugs) which has long been used as an analgesic-inflammatory COX and widely used in the world for treatment of diseases to relieve pain/pain and inflammation such as rheumatoid arthritis, toothache, gout and peripheral muscle pain. In the effort to design and develop new drugs, the first step is modification of commercial drug that has been known its molecular structure and biological activity and was become guidance based on a systematic and rational research to reduce trial and error. Further guidance from lead compounds were developed and modified that become new compounds/derivatives and then was tested this biological activity. Because of extensive use of mefenamic acid so we effort to develop new drugs and created derivatives. Based on previous research, mefenamic acid was substituted benzenesulfonic, bromo anthranilic, paracetamol, phenoxybenzoic, cyclocarboimide the coupling reaction, cyclourea, esters and amides, hydrazine and hidramin can enhance the analgesic effect of anti-inflammatory and reduce the side effects (ulcers). In thi
经硅研究/分子方法预测，甲氧胺酸与4-硝基苯甲酰氯发生苯甲酰化反应，合成了甲氧胺酸衍生物4-硝基苯甲酰甲氧胺酸。合成了甲氧胺酸的衍生物(4- no2 -苯甲酰甲氧胺酸)，以提高其作为镇痛药物/抑制剂COX-2(环氧化酶-2)的候选活性。该化合物经柱层析纯化，并用tlc -密度测定法测定纯度，Rf值为0,8。该斑点具有良好的纯度，然后用400 MHz的H-NMR和FTIR-KBr对该结构进行了鉴定。结果表明，该化合物为4-硝基苯甲酰甲胺酸(4NBMA)。nbma呈白色黄色，熔点198-199C。最后用热板法测定了4-硝基苯甲酰甲胺酸的活性，结果表明4-硝基苯甲酰甲胺酸的活性高于甲胺酸。关键词:硝基苯甲酰甲胺酸，镇痛药，苯甲酰化，分子途径。疼痛是一种多维度的感官体验。国际疼痛研究协会(IASP)将疼痛定义为一种与组织损伤相关的感官和不愉快的情绪体验，包括实际的和潜在的。慢性疼痛是一个严重的问题，如果它增加了疼痛率和慢性患病率。疼痛是最常见的症状之一，在人群中每六个人中就有一个发生疼痛，估计在2-40%的成年人中发生疼痛。一些研究估计，欧洲慢性疼痛的患病率高达55.2%。在印度尼西亚，老年人中有25-50%的人经历过疼痛。慢性疼痛会增加医疗保健费用。美国的研究表明，每年用于慢性疼痛的费用估计在1000亿美元左右。甲氧胺酸作为非甾体抗炎药(NSAIDs, non - steroids AntiInflammatory Drugs，非甾体抗炎药)在市场上早已被用作止痛-炎症的COX，在国际上广泛用于治疗诸如类风湿关节炎、牙痛、痛风、外周肌痛等疾病，以缓解疼痛/疼痛和炎症。在设计和开发新药的过程中，第一步是对已经了解其分子结构和生物活性的商品药物进行修饰，并在系统、合理的研究基础上成为指导，以减少试验和错误。从先导化合物中进一步开发和修饰成新的化合物/衍生物，然后测试其生物活性。由于甲氧胺酸的广泛使用，所以我们努力开发新药和创造衍生物。基于前人的研究，甲氧胺酸被取代苯磺酸、溴苯甲酸、对乙酰氨基酚、苯氧苯甲酸、环碳酰亚胺的偶联反应，环脲、酯类和酰胺类、肼和水牛明可增强抗炎镇痛作用，减少副作用(溃疡)。本研究采用toppliss理论对苯甲酰衍生物进行取代，并用分子方法进行预测。基于Jayaselli等和Susilowati的研究，通过苯甲酰化反应对扑热息痛和吡罗西康衍生物等非甾体抗炎药进行了苯甲酰取代，其生物活性比先导化合物更强。我们使用了几个取代基，苯甲酰衍生物是基于Topliss理论，通过亲脂性，电子和空间参数预测作为取代基。与生物膜渗透速率相关的亲脂性参数。电子参数通过电离和极化过程参与药物与受体相互作用的过程，从而总体上提高了药物的生物有效性。立体参数与化合物与细胞内受体相互作用的相容性有关，它影响最大的结合方向，从而增加活性。亲脂性的提高可以通过插入非极性基团如芳香环来实现，而电子性能的提高可以通过插入电负性取代基如空间卤素来实现。立体位阻特性可以通过创造一个更大的结构来实现，作为一个屏障，促进药物和受体活性位点之间的相互作用。而我们通过增加亲脂性、电子性和立体参数的官能团修饰甲苯胺酸，影响DOI号:10.25258/ ijppr .v9i1.8294 Puspaningtyasm等/ Drug Development of…IJPCR，第9卷，第2期:2017年2月124页生物活性。甲苯甲酰衍生物与甲苯胺酸通过亲核加成反应合成甲苯胺酸衍生物。该机制如图1所示。通过与Molegro虚拟Docker (MVD)对接的分子方法，可以在合成前预测甲苯胺酸衍生物的活性。
{"title":"Drug Development of Mefenamic Acid Derivatives as Analgesic by Molecular Approach","authors":"A. Puspaningtyas","doi":"10.25258/IJPCR.V9I2.8294","DOIUrl":"https://doi.org/10.25258/IJPCR.V9I2.8294","url":null,"abstract":"A new compound of Mefenamic Acid derivate, 4-nitrobenzoyl-mefenamic acid has been synthesized by benzoylation reaction between mefenamic acid and 4-nitrobenzoyl chloride after prediction by in silico study/molecular approach. A derivative of mefenamic acid (4-NO2-benzoyl-mefenamic acid) has been synthesized for increase its activity as candidate of analgesic drug/inhibitor COX-2 (Cyclooxigenase-2). This compound has been purified by Column Chromatography and analyzed using TLC-Densitometry to determine purity with Rf value 0,8. The spot has good purity and then it was identified this structure using H-NMR 400 MHz and FTIR-KBr. The result showed that this compound is 4-nitrobenzoyl-mefenamic acid (4NBMA). 4NBMA gives white yellow color with melting point 198-199C. Finally, 4NBMA was tested analgetic activity by hot plate method and it showed that 4-nitrobenzoyl-mefenamic acid has been higher activity than mefenamic acid. Keyword: 4-nitrobenzoyl-mefenamic acid, analgesic, benzoylation, molecular approach. INTRODUCTION Pain is a multidimentional sensory experience. International Association for the Study of Pain (IASP) defines that pain is a sensory and unpleasant emotional experience associated with tissue damage, both actual and potential. Chronic pain becomes a serious problem if it increases rate of pain and gives chronic prevalence. Pain is one of the most frequently reported symptom occurs in one from six people in the population and it is estimated to occur in 2-40% of adult population. Some studies estimate that the prevalence of chronic pain in Europe is up to 55.2%. In Indonesia, the population of the elderly, reported that 25-50% of them experienced pain. Chronic pain causes increasing health care costs. The research in the United States showed that the cost yearly for chronic pain is estimated around 100 billion dollars. Mefenamic acid is a drug in the market as NSAIDs (Non-steroidal AntiInflammatory Drugs) which has long been used as an analgesic-inflammatory COX and widely used in the world for treatment of diseases to relieve pain/pain and inflammation such as rheumatoid arthritis, toothache, gout and peripheral muscle pain. In the effort to design and develop new drugs, the first step is modification of commercial drug that has been known its molecular structure and biological activity and was become guidance based on a systematic and rational research to reduce trial and error. Further guidance from lead compounds were developed and modified that become new compounds/derivatives and then was tested this biological activity. Because of extensive use of mefenamic acid so we effort to develop new drugs and created derivatives. Based on previous research, mefenamic acid was substituted benzenesulfonic, bromo anthranilic, paracetamol, phenoxybenzoic, cyclocarboimide the coupling reaction, cyclourea, esters and amides, hydrazine and hidramin can enhance the analgesic effect of anti-inflammatory and reduce the side effects (ulcers). In thi","PeriodicalId":19889,"journal":{"name":"药学与临床研究","volume":"67 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87947989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-02-25DOI: 10.25258/IJPCR.V9I2.8290
M. Kishida, Kartika Rahma, S. Prasetyawan, A. Aulanni’am
Several studies have been reported behavioral changes in patients with type 1 diabetes mellitus (T1DM) recently. The increasing levels of glucose affects neuronal activity and may inhibit the process of differentiation and regeneration of neurons in the brain. Dopaminergic (DA) neurons produce dopamine as a neurotransmitter which has an important role to regulate motor functions in the brain. This study aimed to determine the effect of exposure to high concentrations of glucose on the activity of dopaminergic neurons on early development of zebrafish (Danio rerio). Zebrafish embryos were exposed to glucose (1%, 3% and 5%) as a diabetic animal model at the beginning of development. Co-incubation was performed by incubated both of glucose and L-DOPA. Swimming distance of zebrafish larvae was measured as a parameter to determine the motor function regulated by dopamine activity. Results of statistical analysis in zebrafish embryos exposed to 1% and 3% glucose showed significant differences (p less than 0.05) reduction on swimming distance. Co-incubation glucose with L-DOPA partially increased the swimming distance. It can be concluded that the administration of excessive exposure to glucose was able to decrease the activity of dopaminergic neurons by decreasing the distance of swim in early zebrafish development. .
{"title":"Effects of D-Glucose Exposure on Motor Activity by Swimming Distance During Early Development of Zebrafish (Danio rerio)","authors":"M. Kishida, Kartika Rahma, S. Prasetyawan, A. Aulanni’am","doi":"10.25258/IJPCR.V9I2.8290","DOIUrl":"https://doi.org/10.25258/IJPCR.V9I2.8290","url":null,"abstract":"Several studies have been reported behavioral changes in patients with type 1 diabetes mellitus (T1DM) recently. The\u0000increasing levels of glucose affects neuronal activity and may inhibit the process of differentiation and regeneration of\u0000neurons in the brain. Dopaminergic (DA) neurons produce dopamine as a neurotransmitter which has an important role to\u0000regulate motor functions in the brain. This study aimed to determine the effect of exposure to high concentrations of glucose\u0000on the activity of dopaminergic neurons on early development of zebrafish (Danio rerio). Zebrafish embryos were exposed\u0000to glucose (1%, 3% and 5%) as a diabetic animal model at the beginning of development. Co-incubation was performed\u0000by incubated both of glucose and L-DOPA. Swimming distance of zebrafish larvae was measured as a parameter to\u0000determine the motor function regulated by dopamine activity. Results of statistical analysis in zebrafish embryos exposed\u0000to 1% and 3% glucose showed significant differences (p less than 0.05) reduction on swimming distance. Co-incubation glucose\u0000with L-DOPA partially increased the swimming distance. It can be concluded that the administration of excessive exposure\u0000to glucose was able to decrease the activity of dopaminergic neurons by decreasing the distance of swim in early zebrafish\u0000development.\u0000.","PeriodicalId":19889,"journal":{"name":"Pharmaceutical and Clinical Research","volume":"78 7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87921168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-02-25DOI: 10.25258/IJPCR.V9I2.8299
A. Mahmoud, F. Metwally, Hend Rashad, H. Ahmed, Asmaa M. Elfiky, A. Abdalla
Moringa oleifera showed an evident role against obesity and leptin resistance. However, Moringa oleifera potential effects on alteration of moods, cognitive as well as motor deficits in obese animal model have not been evaluated in a mechanistic way. So, the aim of the current study is to examine the potential of Moringa oleifera to induce cerebral leptin mRNA expression, and consequently its effects on amelioration of behavioral and biochemical alterations in obese female rats. Ethanolic extract of Moringa oleifera was orally administered (600 mg/kg b.wt) for 12 weeks to those obese rats. Memory behavior, depressionand anxiety-like behavior, as well as motor activity were examined by object recognition test, forced swim test, light and dark test, and open field test, respectively. Leptin mRNA gene expression and its concentration were determined in cerebral cortex using quantitative real time-PCR and ELISA, respectively. Oxidative stress markers malondialdehyde and glutathione were also evaluated in the cerebral cortex. Moringa oleifera significantly up-regulated cerebral leptin mRNA expression and its level, as well compared to control obese rats. Moreover, Moringa oleifera decreased lipid peroxidation significantly, whereas they improved glutathione significantly in comparison with those untreated rats. Regarding behavioral deficits, Moringa oleifera attenuated the declined memory, depression and anxietylike behavior, as well as the motor deficit that observed in obese untreated rats. This study indicated the potential of Moringa oleifera in triggering cerebral leptin mRNA expression, hence protection of brain from oxidative damage as well as improvement of cognition, moods and motor deficits in obese rats.
{"title":"The Potential of Moringa oleifera to Induce Cerebral Leptin mRNA Expression and to Attenuate Oxidative Stress, Cognitive and Motor Deficits, Depression- and Anxiety- Like Behavior in Experimental Obese Model","authors":"A. Mahmoud, F. Metwally, Hend Rashad, H. Ahmed, Asmaa M. Elfiky, A. Abdalla","doi":"10.25258/IJPCR.V9I2.8299","DOIUrl":"https://doi.org/10.25258/IJPCR.V9I2.8299","url":null,"abstract":"Moringa oleifera showed an evident role against obesity and leptin resistance. However, Moringa oleifera potential effects on alteration of moods, cognitive as well as motor deficits in obese animal model have not been evaluated in a mechanistic way. So, the aim of the current study is to examine the potential of Moringa oleifera to induce cerebral leptin mRNA expression, and consequently its effects on amelioration of behavioral and biochemical alterations in obese female rats. Ethanolic extract of Moringa oleifera was orally administered (600 mg/kg b.wt) for 12 weeks to those obese rats. Memory behavior, depressionand anxiety-like behavior, as well as motor activity were examined by object recognition test, forced swim test, light and dark test, and open field test, respectively. Leptin mRNA gene expression and its concentration were determined in cerebral cortex using quantitative real time-PCR and ELISA, respectively. Oxidative stress markers malondialdehyde and glutathione were also evaluated in the cerebral cortex. Moringa oleifera significantly up-regulated cerebral leptin mRNA expression and its level, as well compared to control obese rats. Moreover, Moringa oleifera decreased lipid peroxidation significantly, whereas they improved glutathione significantly in comparison with those untreated rats. Regarding behavioral deficits, Moringa oleifera attenuated the declined memory, depression and anxietylike behavior, as well as the motor deficit that observed in obese untreated rats. This study indicated the potential of Moringa oleifera in triggering cerebral leptin mRNA expression, hence protection of brain from oxidative damage as well as improvement of cognition, moods and motor deficits in obese rats.","PeriodicalId":19889,"journal":{"name":"Pharmaceutical and Clinical Research","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88875069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-02-25DOI: 10.25258/IJPCR.V9I2.8300
F. Deapsari, T. Erawati, Widji Soeratri
Ubiquinon (Q10) is an endogenous antioxidant. It is lipophilic and practically insoluble in water. To improve its solubility and penetration, it was formulated into O/W nanoemulsion. The resulting Q10 nanoemulsion was then physically characterized for its droplet size, morphology, and viscosity. In addition, it was conducted a penetration test of Q10 in nanoemulsion delivery system compared to Q10 in emulsion using male Wistar rats. From the characterization results, it was found that the droplet size of Q10 nanoemulsion (70.07 ± 12.42 nm) Q10 in emulsion (20.429 μm/h).
{"title":"Penetration of Ubiquinone (Q10) Nanoemulsion Using Olive Oil Through Rat Skin","authors":"F. Deapsari, T. Erawati, Widji Soeratri","doi":"10.25258/IJPCR.V9I2.8300","DOIUrl":"https://doi.org/10.25258/IJPCR.V9I2.8300","url":null,"abstract":"Ubiquinon (Q10) is an endogenous antioxidant. It is lipophilic and practically insoluble in water. To improve its solubility and penetration, it was formulated into O/W nanoemulsion. The resulting Q10 nanoemulsion was then physically characterized for its droplet size, morphology, and viscosity. In addition, it was conducted a penetration test of Q10 in nanoemulsion delivery system compared to Q10 in emulsion using male Wistar rats. From the characterization results, it was found that the droplet size of Q10 nanoemulsion (70.07 ± 12.42 nm) Q10 in emulsion (20.429 μm/h).","PeriodicalId":19889,"journal":{"name":"Pharmaceutical and Clinical Research","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77774391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-02-25DOI: 10.25258/ijpcr.v9i2.8291
K. Bhattacharya, Mahuya Patra Budiati
Background: Carrying heavy loads in the hilly region, porters face extreme ranges in terrain condition, sloped path that is very steep and lack of oxygen due to altitude. The porters in Sikkim, India, routinely carry head-supported loads, which often exceed their body weight, over long distances up and down the steep mountain footpaths which prompted us to assess occupational health status of these head supported load carrying porters. Objective: We aimed our study to evaluate physical and physiological status of male porters of Gangtok, Sikkim. Methods: Seventeen male porters and twenty one healthy as well as age matched (Age: 37 – 52 years) control subjects were selected for the study. Different physical, anthropometric and physiological parameters were studied. Students “t” test was performed to find out the significant difference (P<0.05), if any, between the selected variables. Results: Results of this study on male porters suggest that they are physically healthy but having spinal shrinkage along with lower hand grip strength and PEFR. Conclusion: This study supports the hypothesis that the male porters are associated with decreased lung function and increased spinal shrinkage. To develop an effective intervention strategy, the treatment seeking behavior of individuals would also need to be improved through awareness program.
{"title":"Assessment of Physiological Health Status in Relations to Different Anthropometric and Cardio-Respiratory Measures of Head-Supported Load Carrying Male Porters of Sikkim, India","authors":"K. Bhattacharya, Mahuya Patra Budiati","doi":"10.25258/ijpcr.v9i2.8291","DOIUrl":"https://doi.org/10.25258/ijpcr.v9i2.8291","url":null,"abstract":"Background: Carrying heavy loads in the hilly region, porters face extreme ranges in terrain condition, sloped path that is very steep and lack of oxygen due to altitude. The porters in Sikkim, India, routinely carry head-supported loads, which often exceed their body weight, over long distances up and down the steep mountain footpaths which prompted us to assess occupational health status of these head supported load carrying porters. Objective: We aimed our study to evaluate physical and physiological status of male porters of Gangtok, Sikkim. Methods: Seventeen male porters and twenty one healthy as well as age matched (Age: 37 – 52 years) control subjects were selected for the study. Different physical, anthropometric and physiological parameters were studied. Students “t” test was performed to find out the significant difference (P<0.05), if any, between the selected variables. Results: Results of this study on male porters suggest that they are physically healthy but having spinal shrinkage along with lower hand grip strength and PEFR. Conclusion: This study supports the hypothesis that the male porters are associated with decreased lung function and increased spinal shrinkage. To develop an effective intervention strategy, the treatment seeking behavior of individuals would also need to be improved through awareness program.","PeriodicalId":19889,"journal":{"name":"Pharmaceutical and Clinical Research","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78256636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-02-25DOI: 10.25258/ijpcr.v9i2.8292
Kartika Rahma, M. Kishida, S. Prasetyawan, A. Aulanni’am
Impaired glucose balance affects the regeneration of neurotransmitters in the brain. One of neurotransmitters that is known to have an important role in the brain is dopamine. Dopamine production by dopaminergic (DA) neurons is regulated by the enzyme tyrosine hydroxylase (TH). Previous research has been reported that a decline on TH expression in DA neurons was a result of exposure to excess glucose. One contributing factor is an excess amount of glucose, which interfere homeostasis in the body by producing reactive oxygen species (ROS) more and cause oxidative stress. This condition can cause damage to cells in the brain, including DA neurons, to cell death occurs (apoptosis). The characteristic of neuroprotective owned by the hormone estrogen allegedly able to protect nerve cells from the damaging effects of this. The aim of this study is to explore the involvement of 17β-Estradiol as neuroprotection on the effects of glucose exposure during early development on dopaminergic neurons. This study used zebrafish (Danio rerio) embryos as animal model. They were divided into 4 groups: negative control group, hyperglycemia group, group that was coincubated glucose and estrogen, and the group that only incubated with estrogen alone. Cell death (apoptosis) in the brain was observed using acridine orange staining and TH expression was observed using immunocytochemistry. The results showed that the embryos were exposed to 5% glucose significantly (p <0.05) increased the number of apoptotic cells and decreased the expression of TH. While the results of the analysis of embryos in co-incubation group with estrogen showed improvement with decreased apoptotic cells and the increasing expression of TH up to the normal amount. It can be concluded that estrogen shown to have neuroprotective effect in protecting the decreasing TH expression, as the effects of glucose exposure, which could potentially lead to diabetes, by acting as an antioxidant through cellular mechanisms in the central nervous system.
{"title":"Neuroprotection of 17β-Estradiol Against D-Glucose Exposure Effect on Tyrosine Hydroxylase Expression and Apoptotic Cells in Zebrafish","authors":"Kartika Rahma, M. Kishida, S. Prasetyawan, A. Aulanni’am","doi":"10.25258/ijpcr.v9i2.8292","DOIUrl":"https://doi.org/10.25258/ijpcr.v9i2.8292","url":null,"abstract":"Impaired glucose balance affects the regeneration of neurotransmitters in the brain. One of neurotransmitters that is known to have an important role in the brain is dopamine. Dopamine production by dopaminergic (DA) neurons is regulated by the enzyme tyrosine hydroxylase (TH). Previous research has been reported that a decline on TH expression in DA neurons was a result of exposure to excess glucose. One contributing factor is an excess amount of glucose, which interfere homeostasis in the body by producing reactive oxygen species (ROS) more and cause oxidative stress. This condition can cause damage to cells in the brain, including DA neurons, to cell death occurs (apoptosis). The characteristic of neuroprotective owned by the hormone estrogen allegedly able to protect nerve cells from the damaging effects of this. The aim of this study is to explore the involvement of 17β-Estradiol as neuroprotection on the effects of glucose exposure during early development on dopaminergic neurons. This study used zebrafish (Danio rerio) embryos as animal model. They were divided into 4 groups: negative control group, hyperglycemia group, group that was coincubated glucose and estrogen, and the group that only incubated with estrogen alone. Cell death (apoptosis) in the brain was observed using acridine orange staining and TH expression was observed using immunocytochemistry. The results showed that the embryos were exposed to 5% glucose significantly (p <0.05) increased the number of apoptotic cells and decreased the expression of TH. While the results of the analysis of embryos in co-incubation group with estrogen showed improvement with decreased apoptotic cells and the increasing expression of TH up to the normal amount. It can be concluded that estrogen shown to have neuroprotective effect in protecting the decreasing TH expression, as the effects of glucose exposure, which could potentially lead to diabetes, by acting as an antioxidant through cellular mechanisms in the central nervous system.","PeriodicalId":19889,"journal":{"name":"药学与临床研究","volume":"93 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83464799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-02-25DOI: 10.25258/IJPCR.V9I2.8289
C. Prahasanti, A. Aulanni’am
ABSTRACT Toll-like receptorsplay a role as a bridge between innate immune system and adaptive immune system, playing an important role in body'sdefense against invading germs. Thus, theintroduction of TLR-4can be used as a marker for aggressive and chronic periodontitis. This researchaimed to analyze the expression of TLR-4in patients with aggressive and chronic periodontitis. The samplesin this study were derived from tissue suffering from aggressive and chronic periodontitis. The number of samples in this research was 40 patients with aggressive periodontitis and 40 patients with chronic periodontitis. Then, TLR-4 expression test was conducted by using immunohistochemistry.Based on the results of T-test, a statistical test, it is known that t value was 5.697 with significant value about 0.000 (α = 5%). It is also known that there was significant difference of TLR4 protein expression between in patients with aggressive periodontitis and in those with chronic periodontitis. Morever, based on a box plot diagram, it is known that there was significant difference of TLR4 protein distribution between in patients with aggressive periodontitis and in those with chronic periodontitis. Besides, it is also known that TLR4 protein expression has an effect on the incidence of patients with aggressive periodontitis and chronic periodontitis, as well as the shape of the regression equation. Furthermore, it is known that OR estimated value for the variable expression of TLR4 protein was 1.275 (sign = 0.000). It indicates that if TLR4 protein expression increased 1 (one) unit, then the risk of aggressive periodontitis could be 1,275 times. Conclusion: The increasing of TLR4 protein expression in patients with aggressive periodontitis indicates that cytokine becomes an indicator of inflammation in aggressive periodontitis. Keywords: Toll - like receptors -4, aggressive eriodontitis, chronic periodontitis
{"title":"Toll-Like Receptors as a Potential Marker of Aggressive Periodontitis in Indonesian Population","authors":"C. Prahasanti, A. Aulanni’am","doi":"10.25258/IJPCR.V9I2.8289","DOIUrl":"https://doi.org/10.25258/IJPCR.V9I2.8289","url":null,"abstract":"ABSTRACT \u0000Toll-like receptorsplay a role as a bridge between innate immune system and adaptive immune system, playing an important role in body'sdefense against invading germs. Thus, theintroduction of TLR-4can be used as a marker for aggressive and chronic periodontitis. This researchaimed to analyze the expression of TLR-4in patients with aggressive and chronic periodontitis. The samplesin this study were derived from tissue suffering from aggressive and chronic periodontitis. The number of samples in this research was \u000040 patients with aggressive periodontitis and 40 patients with chronic periodontitis. Then, TLR-4 expression test was conducted by using immunohistochemistry.Based on the results \u0000of T-test, a statistical test, it is known that t value was 5.697 with significant value about 0.000 (α = 5%). It is also known that there was significant difference of TLR4 \u0000protein expression between in patients with aggressive periodontitis and in those with chronic periodontitis. Morever, based on a box plot diagram, it is known that \u0000there was significant difference of TLR4 protein \u0000distribution between in patients with aggressive periodontitis and in those with chronic periodontitis. \u0000Besides, it is also known that TLR4 protein expression \u0000has an effect on the incidence of patients with aggressive periodontitis and chronic periodontitis, \u0000as well as the shape of the regression equation. \u0000Furthermore, it is known that OR estimated value for the variable expression of TLR4 protein was 1.275 (sign = 0.000). \u0000It indicates that if TLR4 protein expression increased 1 (one) unit, then the risk of aggressive periodontitis \u0000could be 1,275 times. \u0000Conclusion: \u0000The increasing of TLR4 protein expression in patients with aggressive periodontitis indicates that cytokine \u0000becomes an indicator of inflammation in aggressive periodontitis. \u0000Keywords: Toll - like receptors -4, aggressive eriodontitis, chronic periodontitis","PeriodicalId":19889,"journal":{"name":"药学与临床研究","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82696832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：