Pub Date : 2023-05-05DOI: 10.18054/pb.v124i3-4.23020
M. Kumar, M. Lonare, A. Telang
Background and purpose: Mycotoxicosis is a very common problem in stored feed and fodder. Ingestion of T-2 toxin through feedstuff leads to serious consequences and production losses in exposed populations. That may lead to reproductive organ system damage resulting in the reproductive problem. The study was planned to investigate the effect of T-2 toxin and vitamin E on testicular function in Wistar rats. Material and methods: Male Wistar rats were fed with standard rat feed. T-2 toxin treatment groups received T-2 toxin (2.5 ppm and 5.0 ppm) mixed feed, while vitamin E (100 mg/kg) was given by oral gavage alone and in T-2 toxin fed groups. After completion of 28 days of oral feeding of text compounds reproductive toxicity parameters were evaluated. Results: Total live sperm counts, sperm motility, and serum testosterone levels were reduced in T-2 toxin-treated groups. Antioxidant enzymes/nonenzymes and oxidative stress indicators were altered significantly and cellular damage to testis and epididymis in T-2 toxin groups was in a dosedependent manner. However, vitamin E intervention caused a marked reversal effect in T-2 toxin-fed rats on testicular damage and male reproductive parameters. Oxidative stress and related events appear to be a potential mechanism involved in T-2 toxin-mediated testicular damage. Conclusion: This study validated that the use of an optimum dose of vitamin E has the potential to protect against the testicular damage caused by the T-2 toxin.
{"title":"Testicular toxicity induced by T-2 toxin and protective effect of vitamin E in Wistar rats","authors":"M. Kumar, M. Lonare, A. Telang","doi":"10.18054/pb.v124i3-4.23020","DOIUrl":"https://doi.org/10.18054/pb.v124i3-4.23020","url":null,"abstract":"Background and purpose: Mycotoxicosis is a very common problem in stored feed and fodder. Ingestion of T-2 toxin through feedstuff leads to serious consequences and production losses in exposed populations. That may lead to reproductive organ system damage resulting in the reproductive problem. The study was planned to investigate the effect of T-2 toxin and vitamin E on testicular function in Wistar rats.\u0000Material and methods: Male Wistar rats were fed with standard rat feed. T-2 toxin treatment groups received T-2 toxin (2.5 ppm and 5.0 ppm) mixed feed, while vitamin E (100 mg/kg) was given by oral gavage alone and in T-2 toxin fed groups. After completion of 28 days of oral feeding of text compounds reproductive toxicity parameters were evaluated.\u0000Results: Total live sperm counts, sperm motility, and serum testosterone levels were reduced in T-2 toxin-treated groups. Antioxidant enzymes/nonenzymes and oxidative stress indicators were altered significantly and cellular damage to testis and epididymis in T-2 toxin groups was in a dosedependent manner. However, vitamin E intervention caused a marked reversal effect in T-2 toxin-fed rats on testicular damage and male reproductive parameters. Oxidative stress and related events appear to be a potential mechanism involved in T-2 toxin-mediated testicular damage.\u0000Conclusion: This study validated that the use of an optimum dose of vitamin E has the potential to protect against the testicular damage caused by the T-2 toxin.","PeriodicalId":19950,"journal":{"name":"Periodicum Biologorum","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46782278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-05DOI: 10.18054/pb.v124i3-4.23435
Analena Robić, D. Habek, M. Prka, Egon Kruezi, D. Plačko-Vršnak, L. Srnec
Background and purpose: The aim of this study was to correlate obstetric admissions at a tertiary perinatal center with biometeorological forecast and weather conditions. Materials and methods: This retrospective study was conducted at the Department of Gynaecology and Obstetrics Clinical Hospital “Sveti Duh” over five years, from January 1, 2014 to December 31, 2018. The hospital’s emergency data was used for record of obstetric admissions on each day. The selected days were sorted in 4 groups based on biometeorological forecast. Results: In the observed period, there were a total of 18,072 obstetric admissions. There were 216 days with fifteen or more admissions. The results showed no significant difference between obstetric admission based on the biometeorological forecast one day before or three days before. Most hospitalization were on days with a favorable biometeorological forecast in the observed period, 68 days with more than fifteen admissions per day. The day before, the biometeorological forecast was mostly favorable or relatively favorable. Conclusion: Our retrospective single-center study did not show a significant difference between obstetric hospital admissions depending on biometeorological conditions, but the higher number of admissions during days with a favorable forecast is definitely the basis for future studies with larger dataset.
{"title":"Correlation between biometeorological forecast and the incidence of obstetric admissions in perinatal center during five years: A retrospective study of one center","authors":"Analena Robić, D. Habek, M. Prka, Egon Kruezi, D. Plačko-Vršnak, L. Srnec","doi":"10.18054/pb.v124i3-4.23435","DOIUrl":"https://doi.org/10.18054/pb.v124i3-4.23435","url":null,"abstract":"Background and purpose: The aim of this study was to correlate obstetric admissions at a tertiary perinatal center with biometeorological forecast and weather conditions.\u0000Materials and methods: This retrospective study was conducted at the Department of Gynaecology and Obstetrics Clinical Hospital “Sveti Duh” over five years, from January 1, 2014 to December 31, 2018. The hospital’s emergency data was used for record of obstetric admissions on each day. The selected days were sorted in 4 groups based on biometeorological forecast.\u0000Results: In the observed period, there were a total of 18,072 obstetric admissions. There were 216 days with fifteen or more admissions. The results showed no significant difference between obstetric admission based on the biometeorological forecast one day before or three days before. Most hospitalization were on days with a favorable biometeorological forecast in the observed period, 68 days with more than fifteen admissions per day. The day before, the biometeorological forecast was mostly favorable or relatively favorable.\u0000Conclusion: Our retrospective single-center study did not show a significant difference between obstetric hospital admissions depending on biometeorological conditions, but the higher number of admissions during days with a favorable forecast is definitely the basis for future studies with larger dataset.","PeriodicalId":19950,"journal":{"name":"Periodicum Biologorum","volume":"1 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67605947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-05DOI: 10.18054/pb.v124i3-4.24697
Tina Petrić, M. Sabol
Tumors are three-dimensional (3D) entities characterized by complex structural architecture which is necessary for adequate intercellular, intracellular and cell-to-matrix interactions among the aberrant cells in cancer. In the field of cancer research, 2D cell cultures are traditionally used for decades in the majority of experiments. The reasons for this are the vast benefits these models provide, including simplicity and cost effectiveness. However, it is now known that these models are exposed to much higher stiffness, they lose physiological extracellular matrix (ECM) on artificial plastic surfaces as well as differentiation, polarization and cell-cell communication. This leads to the loss of crucial cellular signaling pathways and changes in cell responses to stimuli when compared to in vivo conditions. Moreover, they cannot adequately mimic the complexity and dynamic interactions of the tumor microenvironment (TME) which is of great importance in anticancer drug treatments. 3D models seem more biomimetic compared to 2D cell monolayers because they offer the opportunity to model the cancer mass together with its environment which seems the key factor in promoting and directing cancer invasion. 3D cell culture with its additional dimensionality makes the difference in cellular responses because it influences the spatial and physical aspects of the cells in 3D culture. This affects the signal transduction and makes the behavior of 3D-cultured cells more physiologically relevant and reflective of in vivo cellular responses. This review focuses on major differences between 2D and 3D cell cultures, highlighting the importance of considering bioengineering humanized 3D cancer models as the future in cancer research. Additionally, it presents diverse 3D models currently used in cancer research, outlining their benefits and limitations. Precisely, this review highlights the differences between the 3D models with the focus on tumor stroma interactions, cell population and extracellular matrix composition providing methods and examples for each model from the studies done so far.
{"title":"Why 3D in vitro cancer models are the future of cancer research?","authors":"Tina Petrić, M. Sabol","doi":"10.18054/pb.v124i3-4.24697","DOIUrl":"https://doi.org/10.18054/pb.v124i3-4.24697","url":null,"abstract":"Tumors are three-dimensional (3D) entities characterized by complex structural architecture which is necessary for adequate intercellular, intracellular and cell-to-matrix interactions among the aberrant cells in cancer. In the field of cancer research, 2D cell cultures are traditionally used for decades in the majority of experiments. The reasons for this are the vast benefits these models provide, including simplicity and cost effectiveness. However, it is now known that these models are exposed to much higher stiffness, they lose physiological extracellular matrix (ECM) on artificial plastic surfaces as well as differentiation, polarization and cell-cell communication. This leads to the loss of crucial cellular signaling pathways and changes in cell responses to stimuli when compared to in vivo conditions. Moreover, they cannot adequately mimic the complexity and dynamic interactions of the tumor microenvironment (TME) which is of great importance in anticancer drug treatments. 3D models seem more biomimetic compared to 2D cell monolayers because they offer the opportunity to model the cancer mass together with its environment which seems the key factor in promoting and directing cancer invasion. 3D cell culture with its additional dimensionality makes the difference in cellular responses because it influences the spatial and physical aspects of the cells in 3D culture. This affects the signal transduction and makes the behavior of 3D-cultured cells more physiologically relevant and reflective of in vivo cellular responses. This review focuses on major differences between 2D and 3D cell cultures, highlighting the importance of considering bioengineering humanized 3D cancer models as the future in cancer research. Additionally, it presents diverse 3D models currently used in cancer research, outlining their benefits and limitations. Precisely, this review highlights the differences between the 3D models with the focus on tumor stroma interactions, cell population and extracellular matrix composition providing methods and examples for each model from the studies done so far.","PeriodicalId":19950,"journal":{"name":"Periodicum Biologorum","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48502664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-05DOI: 10.18054/pb.v124i3-4.23604
K. Zahradka, J. Repar, Damir Đermić, D. Zahradka
Background and purpose: Homologous recombination in Escherichia coli proceeds via two pathways, RecBCD and RecFOR, which use different enzymes for DNA end resection and loading of RecA recombinase. The postsynaptic reactions following RecA-mediated homologous pairing have mostly been studied within the RecBCD pathway. They involve RuvABC helicase/resolvase complex, RecG and RadA helicases that process recombination intermediates to produce recombinant DNA molecules. Also, RecG functionally interacts with the PriA protein in initiation of recombination-dependent replication. Here, we studied the individual and combined effects of ruvABC, recG and radA null mutations on transductional recombination in both pathways. The effect of the priA300 mutation, which acts as a suppressor of the recG mutation, was also tested. The goal was to characterize the postsynaptic stage of transductional recombination in more details, especially in the RecFOR pathway, which is less well-studied. Materials and methods: Phage P1vir-mediated transduction was used to measure recombination efficiency in a series of recombination mutants. The proA+ marker was used for selection in transductional crosses with various ΔproA recipients. Results: The ruvABC mutation moderately decreased recombination in both recombination pathways, while radA had no effect. The recG mutation reduced recombination in the RecBCD pathway but not in the RecFOR pathway. The strong recombination defect of recG radA double mutants in both pathways was completely suppressed by the priA300 mutation, and this suppression depended on the functional RuvABC complex. Conclusions: RecG and RadA proteins have a redundant role in transductional recombination via RecFOR pathway. In both recombination pathways, RecG and RadA functionally interact with PriA, probably during initiation of recombination-dependent replication.
{"title":"Genetic analysis of transductional recombination in Escherichia coli reveals differences in the postsynaptic stages of RecBCD and RecFOR pathways","authors":"K. Zahradka, J. Repar, Damir Đermić, D. Zahradka","doi":"10.18054/pb.v124i3-4.23604","DOIUrl":"https://doi.org/10.18054/pb.v124i3-4.23604","url":null,"abstract":"Background and purpose: Homologous recombination in Escherichia coli proceeds via two pathways, RecBCD and RecFOR, which use different enzymes for DNA end resection and loading of RecA recombinase. The postsynaptic reactions following RecA-mediated homologous pairing have mostly been studied within the RecBCD pathway. They involve RuvABC helicase/resolvase complex, RecG and RadA helicases that process recombination intermediates to produce recombinant DNA molecules. Also, RecG functionally interacts with the PriA protein in initiation of recombination-dependent replication. Here, we studied the individual and combined effects of ruvABC, recG and radA null mutations on transductional recombination in both pathways. The effect of the priA300 mutation, which acts as a suppressor of the recG mutation, was also tested. The goal was to characterize the postsynaptic stage of transductional recombination in more details, especially in the RecFOR pathway, which is less well-studied.\u0000Materials and methods: Phage P1vir-mediated transduction was used to measure recombination efficiency in a series of recombination mutants. The proA+ marker was used for selection in transductional crosses with various ΔproA recipients.\u0000Results: The ruvABC mutation moderately decreased recombination in both recombination pathways, while radA had no effect. The recG mutation reduced recombination in the RecBCD pathway but not in the RecFOR pathway. The strong recombination defect of recG radA double mutants in both pathways was completely suppressed by the priA300 mutation, and this suppression depended on the functional RuvABC complex.\u0000Conclusions: RecG and RadA proteins have a redundant role in transductional recombination via RecFOR pathway. In both recombination pathways, RecG and RadA functionally interact with PriA, probably during initiation of recombination-dependent replication.","PeriodicalId":19950,"journal":{"name":"Periodicum Biologorum","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44737719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-29DOI: 10.18054/pb.v124i1-2.9922
I. Romero, Mariano del Sol, M. Muñoz
This letter to the editor is a reflection on the historical and etymological origin of the term cell, which is a fundamental term in the biological sciences and we must have knowledge of the official terms associated with this word so widely used in the writing of scientific articles.
{"title":"¿Cytus = Cellula?","authors":"I. Romero, Mariano del Sol, M. Muñoz","doi":"10.18054/pb.v124i1-2.9922","DOIUrl":"https://doi.org/10.18054/pb.v124i1-2.9922","url":null,"abstract":"This letter to the editor is a reflection on the historical and etymological origin of the term cell, which is a fundamental term in the biological sciences and we must have knowledge of the official terms associated with this word so widely used in the writing of scientific articles.","PeriodicalId":19950,"journal":{"name":"Periodicum Biologorum","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43264619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-29DOI: 10.18054/pb.v124i1-2.20578
Amit Kumar Sharma, T. I. Chanu, A. Muralidhar
Background and purpose: The Vibrio spp. are indigenous bacteria in the marine environment and usually constitute the majority in normal microflora of farmed and wild penaeid shrimp; and are one of the most diverse and vital shrimp pathogens that cause heavy mortalities in aquaculture facilities worldwide. The present study aimed to isolate and characterize the causative agent concerned with mass mortality in Peneaus vannamei collected from the shrimp farms of Kakinada, Andhra Pradesh, India.Materials and methods: To isolate and characterize the pathogenic bacteria from the hepatic pancreatic tissue of moribund, P. vannamei performed conventional culture tests, physical and biochemical tests, and molecular analysis. In addition to that virulence gene study, bacterial pathogenicity with different salinity and antibacterial activity of the stem extract of Tinospora cordifolia against Vibrio parahaemolyticus were tested.Results: The phenotypic traits, 16s rRNA gene, and phylogenetic tree analysis showed that the isolated pathogen was V. parahaemolyticus strain KKD 02. A study on bacterial pathogenicity with different salinity 0, 5, 10, 15, 20, and 25 ppt showed that 5 ppt to 10 ppt was connected to the maximum resistance against bacterial infection in P. vannamei with higher CFU/shrimp value. Two genes encoding thermostable direct hemolysin (tdh) and the thermostable direct hemolysin-related hemolysin (trh) were present in the isolate. The antibacterial activity of the stem extract of T. cordifolia, tested against V. parahaemolyticus, revealed that both the ethanol extracts and the crude stem juice exhibited antibacterial activity against V. parahaemolyticus.Conclusion: The cause for the mass mortality of juvenile shrimp P. vannamei in Kakinada, Andhra Pradesh, India, was V. parahaemolyticus strain KKD 02. Further work is necessary to isolate and purify the active constituents in T. cordifolia stem extracts and examine the absorption pattern of the active ingredients of these plants, which will allow the scientific community to recommend their utilization as an accessible alternative to synthetic antibiotics.
{"title":"Detection and pathogenicity of Vibrio parahaemolyticus strains in Penaeus vannamei","authors":"Amit Kumar Sharma, T. I. Chanu, A. Muralidhar","doi":"10.18054/pb.v124i1-2.20578","DOIUrl":"https://doi.org/10.18054/pb.v124i1-2.20578","url":null,"abstract":"Background and purpose: The Vibrio spp. are indigenous bacteria in the marine environment and usually constitute the majority in normal microflora of farmed and wild penaeid shrimp; and are one of the most diverse and vital shrimp pathogens that cause heavy mortalities in aquaculture facilities worldwide. The present study aimed to isolate and characterize the causative agent concerned with mass mortality in Peneaus vannamei collected from the shrimp farms of Kakinada, Andhra Pradesh, India.Materials and methods: To isolate and characterize the pathogenic bacteria from the hepatic pancreatic tissue of moribund, P. vannamei performed conventional culture tests, physical and biochemical tests, and molecular analysis. In addition to that virulence gene study, bacterial pathogenicity with different salinity and antibacterial activity of the stem extract of Tinospora cordifolia against Vibrio parahaemolyticus were tested.Results: The phenotypic traits, 16s rRNA gene, and phylogenetic tree analysis showed that the isolated pathogen was V. parahaemolyticus strain KKD 02. A study on bacterial pathogenicity with different salinity 0, 5, 10, 15, 20, and 25 ppt showed that 5 ppt to 10 ppt was connected to the maximum resistance against bacterial infection in P. vannamei with higher CFU/shrimp value. Two genes encoding thermostable direct hemolysin (tdh) and the thermostable direct hemolysin-related hemolysin (trh) were present in the isolate. The antibacterial activity of the stem extract of T. cordifolia, tested against V. parahaemolyticus, revealed that both the ethanol extracts and the crude stem juice exhibited antibacterial activity against V. parahaemolyticus.Conclusion: The cause for the mass mortality of juvenile shrimp P. vannamei in Kakinada, Andhra Pradesh, India, was V. parahaemolyticus strain KKD 02. Further work is necessary to isolate and purify the active constituents in T. cordifolia stem extracts and examine the absorption pattern of the active ingredients of these plants, which will allow the scientific community to recommend their utilization as an accessible alternative to synthetic antibiotics.","PeriodicalId":19950,"journal":{"name":"Periodicum Biologorum","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43130993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-29DOI: 10.18054/pb.v124i1-2.21463
Seçkin Eroğlu
Background and purpose: Synthetic fertilizers damage the environment. Biofertilizers that consist of microorganisms emerge as an environmentally friendly alternative. Biofertilizers improve plant growth by mobilizing soil nutrients, triggering plant hormone synthesis, or competing with pathogenic bacteria. However, biofertilizers often fail due to insufficient colonization of the plant roots.Materials and methods: To explore the colonization dynamics of a bacterial strain commonly used in biofertilizers, Bacillus subtilis OSU-142 (OSU-142), developing a set of primers specific to OSU-142 was aimed. Since its genome is unknown, to identify genomic regions unique to OSU-142 strain, DNAs of more than 40 bacterial strains were fingerprinted, most of which belong to Bacillus subtilis using the Randomly Amplified Polymorphic DNA (RAPD) method.Results: This approach identified a polymorphic band at 880 bp, which was then cloned and sequenced. The sequence showed no perfect match to any known sequences in the tested genomic databases indicating that the region of OSU-142 DNA is highly unique. The primer was converted to Sequence-Characterized Amplified Region (SCAR) primers, and its functionality in detecting OSU-142 genome was confirmed. Newly designed primer set can specifically detect OSU-142.Conclusions: These primers can be useful for basic science or commercial applications on tracking OSU-142 in various environments, thus contributing to biofertilizers’ adoption in the long run.
{"title":"Development of strain-specific SCAR primers to beneficial rhizobacteria Bacillus subtilis OSU-142","authors":"Seçkin Eroğlu","doi":"10.18054/pb.v124i1-2.21463","DOIUrl":"https://doi.org/10.18054/pb.v124i1-2.21463","url":null,"abstract":"Background and purpose: Synthetic fertilizers damage the environment. Biofertilizers that consist of microorganisms emerge as an environmentally friendly alternative. Biofertilizers improve plant growth by mobilizing soil nutrients, triggering plant hormone synthesis, or competing with pathogenic bacteria. However, biofertilizers often fail due to insufficient colonization of the plant roots.Materials and methods: To explore the colonization dynamics of a bacterial strain commonly used in biofertilizers, Bacillus subtilis OSU-142 (OSU-142), developing a set of primers specific to OSU-142 was aimed. Since its genome is unknown, to identify genomic regions unique to OSU-142 strain, DNAs of more than 40 bacterial strains were fingerprinted, most of which belong to Bacillus subtilis using the Randomly Amplified Polymorphic DNA (RAPD) method.Results: This approach identified a polymorphic band at 880 bp, which was then cloned and sequenced. The sequence showed no perfect match to any known sequences in the tested genomic databases indicating that the region of OSU-142 DNA is highly unique. The primer was converted to Sequence-Characterized Amplified Region (SCAR) primers, and its functionality in detecting OSU-142 genome was confirmed. Newly designed primer set can specifically detect OSU-142.Conclusions: These primers can be useful for basic science or commercial applications on tracking OSU-142 in various environments, thus contributing to biofertilizers’ adoption in the long run.","PeriodicalId":19950,"journal":{"name":"Periodicum Biologorum","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48822282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-29DOI: 10.18054/pb.v124i1-2.20829
M. Prka, D. Habek, Egon Kruezi, D. Plačko-Vršnak, L. Srnec, Kristina Medved
Background and purpose: The effect of weather on people’s well-being and health has been previously noticed and has been a subject of interest for medical professionals and laypeople throughout human history. There are many studies connecting gynecology and obstetrics with weather, some of them investigating the weather and physiological processes such as onset of labor.Materials and methods: In this paper we tried to find relationship between weather conditions and the day with extreme number of deliveries with spontaneous onset (contractions and/or rupture of membranes) in a tertiary referral perinatal center. It is still debatable whether we could connect the weather conditions with actual childbirth.Results: A case analysis shows that there could be a connection between the development of the weather situation and the extreme number of deliveries with spontaneous onset.Conclusion: Unfavorable biometeorological conditions were the result of weather conditions that affect people. In our case there was strong cold advection during the analyzed period, especially on the day with an extreme number of deliveries with spontaneous onset, and significant drop of barometric pressure.
{"title":"Association of weather conditions and the day with extreme number of deliveries with spontaneous onset in a tertiary referral perinatal center","authors":"M. Prka, D. Habek, Egon Kruezi, D. Plačko-Vršnak, L. Srnec, Kristina Medved","doi":"10.18054/pb.v124i1-2.20829","DOIUrl":"https://doi.org/10.18054/pb.v124i1-2.20829","url":null,"abstract":"Background and purpose: The effect of weather on people’s well-being and health has been previously noticed and has been a subject of interest for medical professionals and laypeople throughout human history. There are many studies connecting gynecology and obstetrics with weather, some of them investigating the weather and physiological processes such as onset of labor.Materials and methods: In this paper we tried to find relationship between weather conditions and the day with extreme number of deliveries with spontaneous onset (contractions and/or rupture of membranes) in a tertiary referral perinatal center. It is still debatable whether we could connect the weather conditions with actual childbirth.Results: A case analysis shows that there could be a connection between the development of the weather situation and the extreme number of deliveries with spontaneous onset.Conclusion: Unfavorable biometeorological conditions were the result of weather conditions that affect people. In our case there was strong cold advection during the analyzed period, especially on the day with an extreme number of deliveries with spontaneous onset, and significant drop of barometric pressure.","PeriodicalId":19950,"journal":{"name":"Periodicum Biologorum","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47826722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-29DOI: 10.18054/pb.v124i1-2.20513
D. Đikić, Vedran Balta, S. Pedisić, Z. Zorić, J. Padovan, D. Butorac, A. Milić, D. Jurić, Irena Landeka Jurčević
Background and purpose: Prunus spinose L. is a traditionally consumed, recently scientifically reexamined plant. Brain bioavailability and functionality of polyphenols (PPH) of blackthorn flower extract (PSE) was investigated.Materials and methods: C57BL/6 mice received oral daily repeated doses of 25 mg/kg body weight of total PSE polyphenols for 28 days. Brain concentrations of individual polyphenols from PSE were determined by UPLC/MS on 1st,7th, 14th, 21st, and 28th day. Brain antioxidative defense markers were examined as indicators of functionality after bioaccumulation.Results: A total of 68.7% PPH present in PSE were detected in the brain. Higher (p≤0.05) Cmax/AUClast in the PSE treatment vs. control group was recorded for 59.1% of brain detected compounds, indicating relatively good bioaccumulation in the brain. The highest present compounds in PSE were not necessarily the ones mostly bioapsorbed in the brain. Kaempherols were not significantly distributed, opposite to phenolic acids, quercetins or epicgaloatechin-3-gallate. The compounds with the highest concentrations on 28th day were 4-p-coumaroylquinonic acid, (-)-epicatechin, quercetin-3-O-rutinoside, quercetin–rhamnoside, kaempherol-3-rutinoside and quercetin-3-gucoside. Brain lipid peroxidation (MDA) decreased (p<0.05) on the 21st and 28th day in the PSE group. Increase (p<0.05) in GSH concentration was observed on the 21st and 28th and SOD activity on the 28th day. Catalase activity was unchanged. It could be hypothesized that highest PPH concentration-ratios, caused reduction of lipid peroxidation by radical scavenging and simultaneous induction of glutathione and SOD pathways.Conclusions: Screened compounds could be candidates for examining or creation of brain targeted “neuro-nutriceuticals” polyphenol mixtures.
背景和目的:Prunus spinose L.是一种传统消费，最近科学重新审视的植物。研究了黑刺荆花提取物(PSE)中多酚(PPH)的脑生物利用度和功能。材料与方法:C57BL/6小鼠每日口服总PSE多酚25 mg/kg体重，连续28 d。分别于第1天、第7天、第14天、第21天和第28天采用超高效液相色谱/质谱法测定PSE中各多酚脑内浓度。脑抗氧化防御标志物作为生物积累后功能的指标进行了检测。结果:PSE脑组织中PPH含量为68.7%。与对照组相比，PSE治疗组59.1%的脑检测化合物Cmax/AUClast高于对照组(p≤0.05)，表明PSE治疗组在脑中的生物蓄积相对较好。PSE中含量最高的化合物不一定是那些在大脑中被生物吸收最多的化合物。山柰素分布不明显，而酚酸、槲皮素和表没食子儿茶素-3-没食子酸酯分布不明显。第28天浓度最高的化合物为4-对香豆素醌酸、(-)-表儿茶素、槲皮素-3- o -芦丁苷、槲皮素-鼠李糖苷、山酚-3-芦丁苷和槲皮素-3-葡萄糖苷。PSE组第21、28天脑脂质过氧化(MDA)降低(p<0.05)。第21、28天GSH浓度升高(p<0.05)，第28天SOD活性升高(p<0.05)。过氧化氢酶活性不变。可以推测，最高PPH浓度比通过自由基清除和同时诱导谷胱甘肽和SOD途径引起脂质过氧化的减少。结论:筛选的化合物可作为检测或制造针对大脑的“神经营养品”多酚混合物的候选化合物。
{"title":"Polyphenol bioavailability and modulatory potential on brain antioxidative markers in C57BL/6 mouse","authors":"D. Đikić, Vedran Balta, S. Pedisić, Z. Zorić, J. Padovan, D. Butorac, A. Milić, D. Jurić, Irena Landeka Jurčević","doi":"10.18054/pb.v124i1-2.20513","DOIUrl":"https://doi.org/10.18054/pb.v124i1-2.20513","url":null,"abstract":"Background and purpose: Prunus spinose L. is a traditionally consumed, recently scientifically reexamined plant. Brain bioavailability and functionality of polyphenols (PPH) of blackthorn flower extract (PSE) was investigated.Materials and methods: C57BL/6 mice received oral daily repeated doses of 25 mg/kg body weight of total PSE polyphenols for 28 days. Brain concentrations of individual polyphenols from PSE were determined by UPLC/MS on 1st,7th, 14th, 21st, and 28th day. Brain antioxidative defense markers were examined as indicators of functionality after bioaccumulation.Results: A total of 68.7% PPH present in PSE were detected in the brain. Higher (p≤0.05) Cmax/AUClast in the PSE treatment vs. control group was recorded for 59.1% of brain detected compounds, indicating relatively good bioaccumulation in the brain. The highest present compounds in PSE were not necessarily the ones mostly bioapsorbed in the brain. Kaempherols were not significantly distributed, opposite to phenolic acids, quercetins or epicgaloatechin-3-gallate. The compounds with the highest concentrations on 28th day were 4-p-coumaroylquinonic acid, (-)-epicatechin, quercetin-3-O-rutinoside, quercetin–rhamnoside, kaempherol-3-rutinoside and quercetin-3-gucoside. Brain lipid peroxidation (MDA) decreased (p<0.05) on the 21st and 28th day in the PSE group. Increase (p<0.05) in GSH concentration was observed on the 21st and 28th and SOD activity on the 28th day. Catalase activity was unchanged. It could be hypothesized that highest PPH concentration-ratios, caused reduction of lipid peroxidation by radical scavenging and simultaneous induction of glutathione and SOD pathways.Conclusions: Screened compounds could be candidates for examining or creation of brain targeted “neuro-nutriceuticals” polyphenol mixtures.","PeriodicalId":19950,"journal":{"name":"Periodicum Biologorum","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46172053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-29DOI: 10.18054/pb.v124i1-2.20098
L. Varışlı, Veysel Tolan
Background and purpose: HN1L is a member of the HN1 gene family and shares about 30% similarity with HN1 which is another member of the family on the primary protein sequence. Since HN1 is an important gene that is involved in various cellular mechanisms and also differentially expressed in carcinogenesis, we investigated the effect of HN1L on some malignant behaviors of various cancer cells.Material and methods: Co-expression analysis, Gene Ontology enrichment, and database searches were performed to predict the cellular roles of HN1, and to investigate its expression in cancers and their corresponding normal tissues. Western blotting and Real-Time PCR were used to compare the expression of HN1L in the normal prostate cells and prostate cancer cells. Cell proliferation and migration assays were used to investigate the effects of HN1L depletion on cell proliferation and migration.Results: The results of co-expression and Gene Ontology enrichment analyses showed that HN1L is co-expressed with DNA replication and DNA damage response/repair associated genes. The database search results revealed that HN1L expression increases in at least 10 diverse cancer types compared to their normal corresponding tissues. This result was confirmed in the prostate cancer cell model, experimentally. Silencing of HN1L inhibited proliferative and migrative behaviors of prostate, breast, colon, and cervix cancer cells.Conclusions: HN1L probably is a novel proto-oncogene that is involved in the DNA metabolism-related mechanisms, and high HN1L level promotes further proliferation and migration in the cancer cells.
{"title":"Silencing of HN1L suppresses the proliferation and migration of cancer cells","authors":"L. Varışlı, Veysel Tolan","doi":"10.18054/pb.v124i1-2.20098","DOIUrl":"https://doi.org/10.18054/pb.v124i1-2.20098","url":null,"abstract":"Background and purpose: HN1L is a member of the HN1 gene family and shares about 30% similarity with HN1 which is another member of the family on the primary protein sequence. Since HN1 is an important gene that is involved in various cellular mechanisms and also differentially expressed in carcinogenesis, we investigated the effect of HN1L on some malignant behaviors of various cancer cells.Material and methods: Co-expression analysis, Gene Ontology enrichment, and database searches were performed to predict the cellular roles of HN1, and to investigate its expression in cancers and their corresponding normal tissues. Western blotting and Real-Time PCR were used to compare the expression of HN1L in the normal prostate cells and prostate cancer cells. Cell proliferation and migration assays were used to investigate the effects of HN1L depletion on cell proliferation and migration.Results: The results of co-expression and Gene Ontology enrichment analyses showed that HN1L is co-expressed with DNA replication and DNA damage response/repair associated genes. The database search results revealed that HN1L expression increases in at least 10 diverse cancer types compared to their normal corresponding tissues. This result was confirmed in the prostate cancer cell model, experimentally. Silencing of HN1L inhibited proliferative and migrative behaviors of prostate, breast, colon, and cervix cancer cells.Conclusions: HN1L probably is a novel proto-oncogene that is involved in the DNA metabolism-related mechanisms, and high HN1L level promotes further proliferation and migration in the cancer cells.","PeriodicalId":19950,"journal":{"name":"Periodicum Biologorum","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2022-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49545196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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