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Analysis of the mitochondrial genome to determine the origins and pathways of entry of Angiostrongylus cantonensis in continental Europe (Valencia, Spain). 欧洲大陆(西班牙瓦伦西亚)广东管圆线虫起源和进入途径的线粒体基因组分析。
IF 2.1 3区 医学 Q2 PARASITOLOGY Pub Date : 2024-10-01 Epub Date: 2024-12-02 DOI: 10.1017/S0031182024001318
Mercedes Gómez-Samblás, Beatriz Navarro-Dominguez, Sandra Sáez-Durán, Antonio Osuna, Rubén Bueno-Marí, María Teresa Galán-Puchades, Màrius V Fuentes

Angiostrongylus cantonensis, the rat lungworm, is a zoonotic parasite mainly of rats which act as definitive hosts. If humans become accidentally infected, the nematode is capable of migrating to the brain causing meningoencephalitis. Intermediate hosts are snails and slugs. Although originating from mainland China, A. cantonensis has now spread to various countries and continents. The precise timing of its departure from mainland China remains uncertain although it is often associated with significant historical events or migratory movements. The exit of A. cantonensis from mainland China is believed to have occurred in a singular event, followed by its divergence into 2 distinct clades: clade I, originating from mainland China, and clade II, representing global spread. Angiostrongylus cantonensis was first identified in continental Europe in 2021, specifically in Valencia, Spain. Illumina genome sequencing of 7 individuals isolated from rats captured in 2 different districts in the city of Valencia was carried out. The complete mitochondrial genome was assembled and compared with published A. cantonensis mitochondrial genomes through Bayesian phylogenetic analysis, both for complete mitochondrial genomes and for the cytochrome c oxidase I gene, given its widespread use for identification of the species. The findings revealed the presence of 2 different A. cantonensis haplotypes in the rats studied in Valencia, both belonging to clade II. In 2 rats both clades were present.

广州管圆线虫是一种人畜共患寄生虫,主要寄生于大鼠,是最终宿主。如果人类意外感染,这种线虫能够迁移到大脑,引起脑膜脑炎。中间寄主是蜗牛和蛞蝓。虽然原产于中国大陆,但现已扩散到各个国家和大洲。尽管它经常与重大历史事件或移民运动联系在一起,但它离开中国大陆的确切时间仍不确定。广东南方古猿从中国大陆的离开被认为是一个单一的事件,随后它分裂成两个不同的分支:起源于中国大陆的进化支I和代表全球传播的进化支II。广州管圆线虫于2021年首次在欧洲大陆被发现,特别是在西班牙的瓦伦西亚。对在巴伦西亚市2个不同地区捕获的7只大鼠进行了Illumina基因组测序。考虑到线粒体全基因组被广泛用于物种鉴定,我们通过贝叶斯系统发育分析将完整的线粒体基因组和细胞色素c氧化酶I基因与已发表的广东猿猴线粒体基因组进行了比较。研究结果显示,在瓦伦西亚研究的大鼠中存在2种不同的广东南方古猿单倍型,均属于进化支II。在2只大鼠中,这两个分支都存在。
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引用次数: 0
Isopods infesting Atlantic bonefish (Albula vulpes) host novel viruses, including reoviruses related to global pathogens, and opportunistically feed on humans. 大西洋骨鱼(Albula vulpes)体内的等足类寄生着新型病毒,包括与全球病原体有关的再病毒,并伺机以人类为食。
IF 2.1 3区 医学 Q2 PARASITOLOGY Pub Date : 2024-10-01 Epub Date: 2024-11-20 DOI: 10.1017/S003118202400146X
Tony L Goldberg, Addiel U Perez, Lewis J Campbell

Isopods infest fish worldwide, but their role as disease vectors remains poorly understood. Here, we describe infestation of Atlantic bonefish (Albula vulpes) in Belize with isopods in two of three locations studied, with infestation rates of 15 and 44%. Isopods fed aggressively, and infested fish showed missing scales and scars. Gross morphologic and molecular phylogenetic analyses revealed the isopods to cluster within the family Aegidae and to be most closely related to members of the genus Rocinela, which are globally distributed micro-predators of fish. Metagenomic analysis of 10 isopods identified 11 viruses, including two novel reoviruses (Reovirales) in the families Sedoreoviridae and Spinareoviridae. The novel sedoreovirus clustered phylogenetically within an invertebrate-specific clade of viruses related to the genus Orbivirus, which contains arboviruses of global concern for mammal health. The novel spinareovirus clustered within the fish-infecting genus Aquareovirus, which contains viruses of global concern for fish health. Metagenomic analyses revealed no evidence of infection of bonefish with the novel aquareovirus, suggesting that viremia in bonefish is absent, low, or transient, or that isopods may have acquired the virus from other fish. During field collections, isopods aggressively bit humans, and blood meal analysis confirmed that isopods had fed on bonefish, other fish, and humans. Vector-borne transmission may be an underappreciated mechanism for aquareovirus transmission and for virus host switching between fish and other species, which has been inferred across viral families from studies of deep virus evolution.

等足类动物在世界范围内侵害鱼类,但它们作为疾病媒介的作用仍然知之甚少。在这里,我们描述了伯利兹的大西洋骨鱼(Albula vulpes)在三个研究地点中的两个与等足类动物的侵扰,侵扰率分别为15%和44%。等足类动物进食时很有攻击性,被感染的鱼的鳞片和伤疤都不见了。总体形态学和分子系统发育分析表明,该等足类动物隶属于海燕科,与分布于全球的微捕食者海燕属成员亲缘关系最为密切。对10种等足动物进行宏基因组分析,鉴定出11种病毒,其中包括两种新型呼肠孤病毒(呼肠孤病毒),分别属于塞多呼肠孤病毒科和脊髓呼肠孤病毒科。在系统发育上,这种新型sedoreovirus聚集在与Orbivirus属相关的无脊椎动物病毒分支中,该分支包含全球关注的哺乳动物健康的虫媒病毒。这种新型棘膜病毒聚集在感染鱼类的水膜病毒属中,水膜病毒属中含有全球关注的鱼类健康病毒。宏基因组分析显示,没有证据表明骨鱼感染了这种新型水族病毒,这表明骨鱼的病毒血症是不存在的、低的或短暂的,或者是等足类动物可能从其他鱼类那里获得了病毒。在野外采集过程中,等足类动物积极咬人,血粉分析证实等足类动物以骨鱼、其他鱼类和人类为食。媒介传播可能是水病毒传播和病毒宿主在鱼类和其他物种之间转换的一种未被充分认识的机制,这是通过对病毒深层进化的研究推断出来的。
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引用次数: 0
An evaluation of African animal trypanosomiasis control strategies in remote communities of Eastern Zambia. 评估赞比亚东部偏远社区的非洲动物锥虫病控制策略。
IF 2.1 3区 医学 Q2 PARASITOLOGY Pub Date : 2024-09-01 Epub Date: 2024-10-30 DOI: 10.1017/S0031182024001070
Gloria M Mulenga, Kalinga Chilongo, Chrisborn Mubamba, Bruce Gummow

Communities living in African animal trypanosomiasis (AAT) endemic areas of Zambia use several control strategies to protect their livestock from the devastating effects of trypanosomiasis. Several studies have reported the effectiveness of trypanosomiasis control strategies based on retrospective data. In this study, we assessed incidence rates of AAT in cattle (n = 227) using a prospective cohort study comprising 4 treatment groups, i.e., Diminazene aceturate, Isometamidium chloride, Cyfluthrin pour-on and Cypermethrin treated targets. The study was conducted in Mambwe district in Eastern Zambia between February 2019 and March 2020. The endemic prevalence of AAT for each group was determined using ITS-PCR prior to application of treatments. High endemic trypanosome pre-treatment rates were found in all Groups (Diminazene aceturate (61%), Isometamidium chloride (48%), Cyfluthrin pour-on (87%) and Cypermethrin targets (72%)). The overall apparent prevalence for the Mambwe district was 67% (152/227) and true prevalence at 95%CI was 63–71%. Once treatments were implemented, 12 monthly follow-ups were conducted. The average monthly incidence rates without standardization recorded: Diminazene aceturate (67%) Isometamidium chloride (35%), Cyfluthrin pour-on (55%) and Cypermethrin targets (61%). Incidence rates were standardized considering the endemic level of disease for each Group and the average standardized monthly incidence rate in the Diminazene aceturate Group was 7%; the Isometamidium chloride Group −13%; the Cyfluthrin Group −26%; and the Cypermethrin target Group, −17%. All Groups showed a decrease in incidence of AAT over the period of the study with the Cyfluthrin group showing to be the most effective in reducing AAT incidence in cattle.

生活在赞比亚非洲动物锥虫病(AAT)流行区的社区采用多种控制策略来保护牲畜免受锥虫病的破坏性影响。一些研究根据回顾性数据报告了锥虫病控制策略的有效性。在这项研究中,我们采用了一项前瞻性队列研究,评估了牛(n = 227)的锥虫病发病率,该研究包括 4 个治疗组,即醋酸二咪唑、氯化异戊甲脒、氟氯氰菊酯浇注剂和氯氰菊酯处理目标。研究于 2019 年 2 月至 2020 年 3 月期间在赞比亚东部的曼布韦县进行。在施用处理剂之前,使用 ITS-PCR 确定了每组的锥虫流行率。所有组别(醋酸二咪唑(61%)、氯化异戊甲脒(48%)、氟氯氰菊酯喷洒剂(87%)和氯氰菊酯靶标(72%))的处理前锥虫流行率都很高。曼布韦地区的总体表观流行率为 67%(152/227),95%CI 的真实流行率为 63-71%。实施治疗后,每月进行 12 次随访。记录的月平均发病率没有标准化:乙酸二咪唑(67%)、氯化异戊甲脒(35%)、氟氯氰菊酯(55%)和靶标氯氰菊酯(61%)。考虑到各组疾病的流行程度,对发病率进行了标准化处理,醋酸二咪唑嗪组的平均标准化月发病率为 7%;氯化异戊甲脒组为 -13%;氟氯氰菊酯组为 -26%;氯氰菊酯靶标组为 -17%。在研究期间,所有组别都表明牛的甲胎蛋白发病率有所下降,其中氟氯氰菊酯组在降低牛的甲胎蛋白发病率方面最为有效。
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引用次数: 0
Evaluation of CD1a immunostaining in the diagnosis of cutaneous leishmaniasis caused by Leishmania donovani in Sri Lanka. 评估 CD1a 免疫染色法在诊断斯里兰卡由多诺万利什曼病引起的皮肤利什曼病中的应用。
IF 2.1 3区 医学 Q2 PARASITOLOGY Pub Date : 2024-09-01 Epub Date: 2024-11-11 DOI: 10.1017/S0031182024000799
Hasna Riyal, Nilakshi Samaranayake, Priyani Amarathunga, Deepani Munidasa, Nadira Karunaweera

Cutaneous leishmaniasis (CL) is a vector-borne parasitic disease, routinely diagnosed by direct light microscopy. The sensitivity of this method is dependent on the number of parasites present in the lesion. Immunoexpression of CD1a surface antigen by Leishmania amastigotes and its application as a diagnostic tool has been recently demonstrated in several species including Leishmania major, Leishmania tropica and Leishmania infantum. Leishmania donovani is the only reported species in Sri Lanka primarily causing CL and its CD1a status remains unexplored. We studied CD1a expression by amastigotes of L. donovani in skin biopsies from 116 patients with suspected CL. The biopsy sections were stained with CD1a clones O10 and MTB1 separately. Slit skin smear (SSS) results were considered the gold standard for diagnosis of CL. 103 cases were confirmed through SSS where 73 of them showed positive parasite staining for CD1a clone MTB1 with 70.9% sensitivity. Positivity was seen mostly in parasites closer to the epidermis. CD1a clone O10 failed to detect any amastigotes. Test sensitivity improved to 74.1% when the analysis was applied only to patients with low/no discernible Leishman-Donovan (LD) bodies in histology. Our findings show that CD1a clone MTB1 successfully stains amastigotes of L. donovani species and can be used as a supplementary diagnostic tool in detecting CL, especially when LD bodies are low in number. This method could be validated to detect other forms of leishmaniasis caused by L. donovani in Indian and sub-Saharan regions.

皮肤利什曼病(CL)是一种病媒传播的寄生虫病,常规诊断方法是直接光学显微镜检查。这种方法的灵敏度取决于病变部位寄生虫的数量。利什曼原虫对 CD1a 表面抗原的免疫表达及其作为诊断工具的应用最近已在包括大利什曼原虫、热带利什曼原虫和婴儿利什曼原虫在内的多个物种中得到证实。多诺万利什曼原虫是斯里兰卡唯一报道的主要引起CL的原虫,但其CD1a状态仍未得到研究。我们研究了116名疑似CL患者的皮肤活检组织中唐诺瓦利什曼原虫的CD1a表达情况。活检切片分别用 CD1a 克隆 O10 和 MTB1 染色。裂隙皮肤涂片(SSS)结果被视为诊断 CL 的金标准。103 个病例通过 SSS 得到确诊,其中 73 个病例的 CD1a 克隆 MTB1 寄生虫染色呈阳性,灵敏度为 70.9%。阳性寄生虫主要出现在靠近表皮的部位。CD1a 克隆 O10 未能检测到任何母虫。如果仅对组织学检查中出现低度/无明显莱什曼-多诺万(LD)体的患者进行分析,检测灵敏度将提高到 74.1%。我们的研究结果表明,CD1a 克隆 MTB1 能成功染色唐诺瓦尼氏菌的非膜体,可用作检测 CL 的辅助诊断工具,尤其是当 LD 体数量较少时。这种方法可用于检测印度和撒哈拉以南地区由唐诺万尼利什曼病引起的其他形式的利什曼病。
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引用次数: 0
Molecular characterization and B-cell epitope analysis of the TSP11 gene in Echinococcus infection strains from Yunnan Province. 云南省棘球蚴感染株 TSP11 基因的分子特征和 B 细胞表位分析。
IF 2.1 3区 医学 Q2 PARASITOLOGY Pub Date : 2024-09-01 Epub Date: 2024-11-12 DOI: 10.1017/S0031182024000726
Qian Xu, Zhengqing Wang, Jinrong Zi, Xuan Cai, Fangwei Wu, Benfu Li, Jia Peng, Jianxiong Li, Xinliu Yan, Ying Dong, Yaming Yang

This study investigates the molecular intricacies of the transmembrane protein TSP11 gene in Echinococcus strains isolated from livestock and patients in Yunnan Province afflicted with Echinococcus granulosus (E. granulosus) between 2016 and 2020. Gene typing analysis of the ND1 gene revealed the presence of the G1 type, G5 type and untyped strains, constituting 52.4, 38.1 and 9.5%, respectively. The analysis of 42 DNA sequences has revealed 24 novel single nucleotide polymorphic sites, delineating 11 haplotypes, all of which were of the mutant type. Importantly, there were no variations observed in mutation sites or haplotypes in any of the hosts. The total length of the TSP11 gene's 4 exons is 762 bp, encoding 254 amino acids. Our analysis posits the existence of 6 potential B-cell antigenic epitopes within TSP11, specifically at positions 49-KSN-51, 139-GKRG-142, 162-DNG-164, 169-NGS-171, 185-DS-186 and 231-PPRFTN-236. Notably, these epitopes exhibit consistent presence among various intermediate hosts and haplotypes. However, further validation is imperative to ascertain their viability as diagnostic antigens for E. granulosus in the Yunnan Province.

本研究调查了2016年至2020年期间从云南省感染粒细胞棘球蚴病(E. granulosus)的家畜和患者中分离的棘球蚴菌株中跨膜蛋白TSP11基因的分子复杂性。对 ND1 基因的基因分型分析显示,存在 G1 型、G5 型和未分型菌株,分别占 52.4%、38.1% 和 9.5%。对 42 个 DNA 序列的分析发现了 24 个新的单核苷酸多态性位点,划分出 11 个单倍型,所有单倍型均为突变型。重要的是,在任何宿主中都没有观察到突变位点或单倍型的变化。TSP11 基因的 4 个外显子总长度为 762 bp,编码 254 个氨基酸。我们的分析推测 TSP11 中存在 6 个潜在的 B 细胞抗原表位,具体位于 49-KSN-51、139-GKRG-142、162-DNG-164、169-NGS-171、185-DS-186 和 231-PPRFTN-236 位。值得注意的是,这些表位在不同的中间宿主和单倍型中表现出一致的存在。然而,要确定这些抗原作为云南省粒细胞埃希氏菌诊断抗原的可行性,还需要进一步验证。
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引用次数: 0
The first mitogenome of the genus Amphalius (Siphonaptera: Ceratophyllidae) and its phylogenetic implications. 双翅虫属的第一个有丝分裂基因组及其系统发育意义。
IF 2.1 3区 医学 Q2 PARASITOLOGY Pub Date : 2024-09-01 Epub Date: 2024-12-03 DOI: 10.1017/S0031182024000635
Ju Pu, Xiaoxia Lin, Wenge Dong

Amphalius spirataenius belongs to Arthropoda, Insecta, Siphonaptera, Ceratophylloidea, Ceratophyllinae, Amphalius. Only 2 species from the subfamily Ceratophyllinae have been sequenced for mitogenomes to date. The genus Amphalius mitogenome research was still blank. The A. spirataenius mitogenome was determined, annotated and analysed for the first time in this study. The 14 825 bp long genome has the typical metazoan of 37 genes with insect ancestral genome arrangement pattern. There was no significant difference in codon usage of 13 protein-coding genes: UUA, UCU, GUU, ACU and GCU were the most frequently used codons. It was found that the reason for codon preference mainly contributed to natural selection base on PR2, ENC-plot and neutrality curve analysis. Evolutionary rate, conserved sites, variable sites and nucleotide diversity analysis indicated that nad6 of A. spirataenius had the fastest evolutionary rate, while cox1 had the slowest evolutionary rate. Phylogenetic trees were reconstructed based on 13 protein-coding genes and 2 rRNA genes datasets using Bayesian inference and maximum likelihood method. The phylogenetic tree supported that both Siphonaptera and Mecoptera were monophyletic, and were sister groups to each other. This study filled gap of the genus Amphalius mitogenome sequences and was of great significance for understanding evolution of the order Siphonaptera.

螺旋藻属节肢动物、昆虫亚目、吸翅目、角藻亚目、角藻亚目、角藻亚目。迄今为止,仅有2种角鼻苔亚科植物进行了有丝分裂基因组测序。对双足属有丝分裂基因组的研究尚属空白。本研究首次对螺旋藻有丝分裂基因组进行了测定、注释和分析。全长14 825 bp的基因组具有37个基因的典型后生动物,具有昆虫祖先基因组排列模式。13个蛋白编码基因的密码子使用差异无统计学意义,UUA、UCU、GUU、ACU和GCU是使用频率最高的密码子。基于PR2、c -plot和中性曲线分析,发现密码子偏好的原因主要是自然选择。进化速率、保守位点、可变位点和核苷酸多样性分析表明,螺旋藻nad6进化速度最快,cox1进化速度最慢。基于13个蛋白质编码基因和2个rRNA基因数据集,利用贝叶斯推理和极大似然方法重构了系统发育树。系统发育树支持管翅目和甲翅目为单系,互为姊妹类群。该研究填补了双翅虫属有丝分裂基因组序列的空白,对了解管翅目昆虫的进化具有重要意义。
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引用次数: 0
A first record of a suspected intestinal myiasis caused by Muscina stabulans (Fallén 1817) (Diptera: Muscidae) in Southern South America. 在南美洲南部首次记录到由 Muscina stabulans (Fallén 1817) (双翅目:鹟科)引起的疑似肠肌炎。
IF 2.1 3区 医学 Q2 PARASITOLOGY Pub Date : 2024-09-01 Epub Date: 2024-10-23 DOI: 10.1017/S0031182024001094
Fernando H Aballay, Marta I Saloña-Bordas, M Alejandra Perotti

We report a case of a suspected intestinal myiasis of a child from Southeast Argentina. Diptera larvae were sampled by a physician from the nappy worn by the child and submitted for examination and identification to the Laboratorio de Artrópodos (Universidad Nacional de Mar del Plata). Based on diagnosis of the anterior and posterior spiracles and mouthparts, the larvae were identified as the false stable fly Muscina stabulans (Fallén, 1817) (Diptera: Muscidae). A comparison of diagnostic characters between Argentinean and European third instars of this species is presented. Muscina stabulans is a prevalent species in the district of General Pueyrredón, Buenos Aires province, where the case occurred. Its abundance in the area coincidentally peaked at the time of the infestation. This is the 1st report of M. stabulans as a suspect of intestinal myiasis for the whole of the southern cone of South America (Chile and Argentina).

我们报告了一例阿根廷东南部儿童疑似肠道蠅蛆病的病例。医生从孩子穿的尿布上采集了双翅目幼虫样本,并提交给马德普拉塔国立大学(Universidad Nacional de Mar del Plata)的Artrópodos实验室进行检查和鉴定。根据对前后螺线管和口器的诊断,幼虫被确定为假厩蝇 Muscina stabulans(Fallén,1817 年)(双翅目:鹟科)。本文对该物种阿根廷和欧洲第三龄幼虫的诊断特征进行了比较。Muscina stabulans 是该病例发生地布宜诺斯艾利斯省 General Pueyrredón 地区的常见物种。它在该地区的数量恰好在虫害发生时达到高峰。这是整个南美洲南锥体地区(智利和阿根廷)首次报告 M. stabulans 是肠道蠅蛆病的疑似病例。
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引用次数: 0
A new blood parasite of the accentor birds: description, molecular characterization, phylogenetic relationships and distribution. 一种新的鸟类血液寄生虫:描述、分子特征、系统发育关系和分布。
IF 2.1 3区 医学 Q2 PARASITOLOGY Pub Date : 2024-09-01 Epub Date: 2024-11-15 DOI: 10.1017/S0031182024000878
Gediminas Valkiūnas, Tatjana A Iezhova, Mélanie Duc, Jenny C Dunn, Staffan Bensch

Haemoproteus bobricklefsi sp. nov. (Haemosporida, Haemoproteidae) was found in the dunnock Prunella modularis and represents the first blood parasite described in accentor birds of the Prunellidae. The description is based on the morphology of blood stages and includes information about a barcoding segment of the mitochondrial cytochrome b gene (lineage hDUNNO01) and the full mitochondrial genome, which can be used for identification and diagnosis of this infection. The new parasite can be readily distinguished from described species of haemoproteids parasitizing passeriform birds due to markedly variable position of nuclei in advanced and fully grown macrogametocytes. Illustrations of blood stages of the new species are given, and phylogenetic analyses based on partial mitochondrial cytochrome b gene sequences and the full mitochondrial genome identified the closely related lineages. DNA haplotype networks showed that transmission occurs in Europe and North America. This parasite was found in the dunnock in Europe and several species of the Passerellidae in North America. It is probably of Holarctic distribution, with the highest reported prevalence in the UK. The parasite distribution seems to be geographically patchy, with preference for areas of relatively cool climates. Phylogenetic analysis suggests that H. bobricklefsi sp. nov. belongs to the Parahaemoproteus subgenus and is probably transmitted by biting midges belonging to Culicoides (Ceratopogonidae). The available data on molecular occurrence indicate that this pathogen is prone to abortive development, so worth attention in regard of consequences for bird health.

Haemoproteus bobricklefsi sp.nov.(血孢子虫科,Haemosporida, Haemoproteidae)被发现于杜父鱼(Prunella modularis)中,是首次在杜父鱼科(Prunellidae)口音鸟类中描述的血液寄生虫。该描述以血液阶段的形态为基础,包括线粒体细胞色素 b 基因的条形码片段(hDUNNO01 系)和完整的线粒体基因组信息,可用于鉴定和诊断这种感染。由于这种新寄生虫在晚期和完全生长的大核细胞中细胞核的位置明显不同,因此可以很容易地将其与已描述过的寄生于雀形目鸟类的血蛋白寄生虫区分开来。文中给出了新物种血液阶段的图解,并根据部分线粒体细胞色素 b 基因序列和完整的线粒体基因组进行了系统发育分析,确定了密切相关的品系。DNA 单倍型网络显示,该寄生虫在欧洲和北美洲传播。这种寄生虫在欧洲的杜父鱼和北美洲的几种雀形目鱼类中都有发现。该寄生虫可能分布在北半球,英国的报告流行率最高。寄生虫的地理分布似乎不均衡,偏好气候相对凉爽的地区。系统发生学分析表明,H. bobricklefsi sp.现有的分子发生数据表明,这种病原体很容易流产,因此值得关注其对鸟类健康的影响。
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引用次数: 0
Unravelling the diversity of Posthodiplostomum Dubois, 1936 (Trematoda: Diplostomidae) in fish-eating birds from the Neotropical region of Mexico, with the description of a new species. 揭示墨西哥新热带地区食鱼鸟类中 Posthodiplostomum Dubois, 1936 (Trematoda: Diplostomidae) 的多样性,并描述一个新物种。
IF 2.1 3区 医学 Q2 PARASITOLOGY Pub Date : 2024-09-01 Epub Date: 2024-11-11 DOI: 10.1017/S0031182024000970
Marcelo Tonatiuh González-García, Alejandra López-Jiménez, Mirza Patricia Ortega-Olivares, Ana Lucia Sereno-Uribe, Gerardo Pérez-Ponce de León, Martín García-Varela

Adults of the genus Posthodiplostomum, Dubois, 1936 are parasites of fish-eating birds, mainly of the family Ardeidae, and are globally distributed. The genus currently comprises 35 species, although recent molecular evidence has shown that the diversity of the genus is underestimated since several candidate species have been recognized. In the Neotropical region of Mexico, at least 6 Posthodiplostomum lineages have been detected with metacercaria stages recovered from unrelated fish hosts. Here, we obtained adult specimens of Posthodiplostomum from 6 fish-eating birds representing 2 families (Butorides virescens, Ardea herodias, Nycticorax nycticorax, Tigrisoma mexicanum – Ardeidae, and Rynchops niger and Leucophaeus atricilla – Lariidae) from 4 localities in southern Mexico. Specimens were sequenced for 2 nuclear (28S and ITS1–5.8S–ITS2) and 1 mitochondrial (cox1) molecular marker. Phylogenetic analyses allowed us to link metacercariae and adult specimens and recognized a lineage, which was described morphologically. The new species can be distinguished from its congeners by its prosoma morphology and body size; this is the first described species in the Neotropical region of Mexico. Additionally, new host and locality records for P. macrocotyle and P. pricei are presented, expanding their geographical distribution range in the Americas.

Posthodiplostomum, Dubois, 1936 年属的成虫寄生于食鱼鸟类,主要是杓鹬科的鸟类,分布于全球各地。该属目前有 35 个种,但最近的分子证据表明,该属的多样性被低估了,因为有几个候选种已被确认。在墨西哥的新热带地区,从不相干的鱼类宿主身上发现了至少 6 个 Posthodiplostomum 品系的 metacercaria 阶段。在这里,我们从墨西哥南部 4 个地方的 2 个科(Butorides virescens、Ardea herodias、Nycticorax nycticorax、Tigrisoma mexicanum - Ardeidae,以及 Rynchops niger 和 Leucophaeus atricilla - Lariidae)的 6 种食鱼鸟类身上获得了 Posthodiplostomum 的成体标本。对标本的 2 个核标记(28S 和 ITS1-5.8S-ITS2)和 1 个线粒体标记(cox1)进行了测序。系统发育分析使我们能够将元包虫和成虫标本联系起来,并确认了一个世系,对该世系进行了形态学描述。该新物种可通过其前口形态和体型与其同属物种区分开来;这是墨西哥新热带地区首次描述的物种。此外,还提供了 P. macrocotyle 和 P. pricei 的新寄主和地点记录,扩大了它们在美洲的地理分布范围。
{"title":"Unravelling the diversity of <i>Posthodiplostomum</i> Dubois, 1936 (Trematoda: Diplostomidae) in fish-eating birds from the Neotropical region of Mexico, with the description of a new species.","authors":"Marcelo Tonatiuh González-García, Alejandra López-Jiménez, Mirza Patricia Ortega-Olivares, Ana Lucia Sereno-Uribe, Gerardo Pérez-Ponce de León, Martín García-Varela","doi":"10.1017/S0031182024000970","DOIUrl":"10.1017/S0031182024000970","url":null,"abstract":"<p><p>Adults of the genus <i>Posthodiplostomum</i>, Dubois, 1936 are parasites of fish-eating birds, mainly of the family Ardeidae, and are globally distributed. The genus currently comprises 35 species, although recent molecular evidence has shown that the diversity of the genus is underestimated since several candidate species have been recognized. In the Neotropical region of Mexico, at least 6 <i>Posthodiplostomum</i> lineages have been detected with metacercaria stages recovered from unrelated fish hosts. Here, we obtained adult specimens of <i>Posthodiplostomum</i> from 6 fish-eating birds representing 2 families (<i>Butorides virescens</i>, <i>Ardea herodias</i>, <i>Nycticorax nycticorax</i>, <i>Tigrisoma mexicanum</i> – Ardeidae, and <i>Rynchops niger</i> and <i>Leucophaeus atricilla</i> – Lariidae) from 4 localities in southern Mexico. Specimens were sequenced for 2 nuclear (28S and ITS1–5.8S–ITS2) and 1 mitochondrial (<i>cox1</i>) molecular marker. Phylogenetic analyses allowed us to link metacercariae and adult specimens and recognized a lineage, which was described morphologically. The new species can be distinguished from its congeners by its prosoma morphology and body size; this is the first described species in the Neotropical region of Mexico. Additionally, new host and locality records for <i>P. macrocotyle</i> and <i>P. pricei</i> are presented, expanding their geographical distribution range in the Americas.</p>","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"1225-1241"},"PeriodicalIF":2.1,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11894011/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Complete mitochondrial genome of Scathophaga stercoraria (Diptera: Scathophagidae) in wild plateau pika: Genome descriptions and Phylogenetic evolution - CORRIGENDUM. 野生高原鼠兔 Scathophaga stercoraria(双翅目:Scathophagidae)的完整线粒体基因组:Genome descriptions and Phylogenetic evolution - CORRIGENDUM.
IF 2.1 3区 医学 Q2 PARASITOLOGY Pub Date : 2024-09-01 Epub Date: 2024-11-21 DOI: 10.1017/S003118202400115X
Haining Zhang, Wangkai Chen, Ru Meng, Hong Duo, Xueyong Zhang, Zhihong Guo, Xiuying Shen, Qing Liu, Zhi Li, Yong Fu
{"title":"Complete mitochondrial genome of <i>Scathophaga stercoraria</i> (Diptera: Scathophagidae) in wild plateau pika: Genome descriptions and Phylogenetic evolution - CORRIGENDUM.","authors":"Haining Zhang, Wangkai Chen, Ru Meng, Hong Duo, Xueyong Zhang, Zhihong Guo, Xiuying Shen, Qing Liu, Zhi Li, Yong Fu","doi":"10.1017/S003118202400115X","DOIUrl":"10.1017/S003118202400115X","url":null,"abstract":"","PeriodicalId":19967,"journal":{"name":"Parasitology","volume":" ","pages":"1174"},"PeriodicalIF":2.1,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11893994/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Parasitology
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