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Acute Consumption of Cooked Green Banana Pulp Beverage (Musa cavendishii) Decreases Plasma Glucose in Healthy Women: A Cross-Sectional Controlled Study. 急性饮用煮熟的绿色香蕉果肉饮料(Musa cavendishii)可降低健康女性的血浆葡萄糖:一项横断面对照研究
IF 3.1 2区 农林科学 Q2 CHEMISTRY, APPLIED Pub Date : 2024-09-01 Epub Date: 2024-07-01 DOI: 10.1007/s11130-024-01202-w
Lilia Zago, Heloisa Rodrigues Pessoa, Carolyne Pimentel Rosado, Andreia Ana da Silva, Antonella Pasqualone, Josely Correa Koury

This study aimed at comparing the carbohydrate composition of three banana varieties (cv. Nanica, Nanicão, and Prata) and investigating the effect of a single dose of cooked green banana pulp beverage (GBPd) on plasma glycemic homeostasis indexes (glucose, PYY, GIP, insulin) and hunger and satiety sensation (visual analog scale-VAS). The bananas were classified according to the color scale. The fiber, total carbohydrate, and resistant starch (RS) were determined using validated methods. Glucose homeostasis indexes and hunger/satiety sensation were determined in ten healthy women in two stages before and after intake: (1) glucose solution (250 g/L); (2) one week later, consumption of the glucose solution plus 75 g/L of GBPd. Blood samples were collected twice in stage-1 and every 15 min for 2 h in stage-2. Cv. Nanicão was selected, because it presented a higher content in RS and dietary fiber on dry base than the other cultivars. Thus, it was used to test glycemic response. After 2 h of GBPd intake, no difference was observed in hunger/satiety sensation and plasma glycemic homeostasis indexes, except for a decrease in plasma glucose concentration (-15%, p = 0.0232) compared to stage-1. These results suggest that cv. Nanicão has a higher potential as a functional ingredient and can influence the reduction in the glycemic index of a meal compared to other cultivars. However, it had not a short-term effect on hormones GIP and PYY in healthy women. Further research is needed to understand the long-term effects and mechanisms of green banana on glycemic control and satiety.

本研究旨在比较三个香蕉品种(Nanica、Nanicão和Prata)的碳水化合物组成,并调查单剂量煮熟的绿色香蕉果肉饮料(GBPd)对血浆血糖平衡指数(葡萄糖、PYY、GIP、胰岛素)以及饥饿和饱腹感(视觉模拟量表-VAS)的影响。香蕉根据色标进行分类。纤维、总碳水化合物和抗性淀粉(RS)均采用有效方法测定。10 名健康女性在摄入前后分两个阶段测定了葡萄糖稳态指数和饥饿/饱腹感:(1)葡萄糖溶液(250 克/升);(2)一周后,摄入葡萄糖溶液加 75 克/升的 GBPd。第一阶段采集两次血样,第二阶段在 2 小时内每 15 分钟采集一次血样。选择 Cv.之所以选择 Nanicão 品种,是因为该品种的 RS 和膳食纤维含量高于其他品种。因此,它被用来测试血糖反应。摄入 GBPd 2 小时后,除了血浆葡萄糖浓度下降(-15%,p = 0.0232)外,饥饿/饱腹感和血浆血糖平衡指数与第一阶段相比没有差异。这些结果表明,Cv.与其他栽培品种相比,Nanicão 作为功能性配料的潜力更大,可影响膳食血糖生成指数的降低。不过,它对健康女性体内的荷尔蒙 GIP 和 PYY 没有短期影响。要了解青香蕉对血糖控制和饱腹感的长期影响和机制,还需要进一步的研究。
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引用次数: 0
Andean Crops Germination: Changes in the Nutritional Profile, Physical and Sensory Characteristics. A Review. 安第斯作物的发芽:营养成分、物理和感官特征的变化。综述。
IF 3.1 2区 农林科学 Q2 CHEMISTRY, APPLIED Pub Date : 2024-09-01 Epub Date: 2024-07-08 DOI: 10.1007/s11130-024-01209-3
M D Jiménez, C E Salinas Alcón, M O Lobo, N Sammán

Andean crops such as quinoa, amaranth, cañihua, beans, maize, and tarwi have gained interest in recent years for being gluten-free and their high nutritional values; they have high protein content with a well-balanced essential amino acids profile, minerals, vitamins, dietary fiber, and antioxidant compounds. During the germination bioprocess, the seed metabolism is reactivated resulting in the catabolism and degradation of macronutrients and some anti-nutritional compounds. Therefore, germination is frequently used to improve nutritional quality, protein digestibility, and availability of certain minerals and vitamins; furthermore, in specific cases, biosynthesis of new bioactive compounds could occur through the activation of secondary metabolic pathways. These changes could alter the technological and sensory properties, such as the hardness, consistency and viscosity of the formulations prepared with them. In addition, the flavor profile may undergo improvement or alteration, a critical factor to consider when integrating sprouted grains into food formulations. This review summarizes recent research on the nutritional, technological, functional, and sensory changes occur during the germination of Andean grains and analyze their potential applications in various food products.

近年来,藜麦、苋菜、卡尼花、豆类、玉米和塔尔维等安第斯作物因不含麸质和营养价值高而备受关注;这些作物蛋白质含量高,含有均衡的必需氨基酸、矿物质、维生素、膳食纤维和抗氧化化合物。在萌芽生物过程中,种子的新陈代谢被重新激活,导致宏量营养素和一些抗营养化合物的分解和降解。因此,萌发经常被用来改善营养质量、蛋白质消化率以及某些矿物质和维生素的可获得性;此外,在特定情况下,还可通过激活次级代谢途径来进行新生物活性化合物的生物合成。这些变化可能会改变技术和感官特性,如用它们配制的配方的硬度、稠度和粘度。此外,风味可能会得到改善或改变,这是将发芽谷物融入食品配方时需要考虑的一个关键因素。本综述总结了有关安第斯谷物发芽过程中营养、技术、功能和感官变化的最新研究,并分析了它们在各种食品中的潜在应用。
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引用次数: 0
The Protective Effect of Juçara Fruit (Euterpe edulis Martius) Extracts on LPS-Activated J774 Macrophages. Juçara 果实(Euterpe edulis Martius)提取物对 LPS 激活的 J774 巨噬细胞的保护作用
IF 3.1 2区 农林科学 Q2 CHEMISTRY, APPLIED Pub Date : 2024-09-01 Epub Date: 2024-07-08 DOI: 10.1007/s11130-024-01204-8
Mayara Schulz, Luciano Valdemiro Gonzaga, Ana Clara Nascimento Antunes, Tainá Lubschinski, Eduarda Talita Bramorski Mohr, Eduardo Monguilhott Dalmarco, Carolina Turnes Pasini Deolindo, Rodrigo Barcellos Hoff, Fábio Martinho Zambonim, Ana Carolina Oliveira Costa, Roseane Fett

This study investigated the anti-inflammatory effect of hydrophilic and lipophilic extracts from juçara fruits (Euterpe edulis Martius) through measurement of nitric oxide (NOx) and cytokines (IL-12p70, TNF-α, INF-γ, MCP-1, IL-6, and IL-10). J774 macrophages were stimulated with lipopolysaccharides (1 µg/mL) and treated with various concentrations (1-100 µg/mL) of juçara fruits extracts from crude extracts, and hexane, dichloromethane, ethyl acetate, and butanol fractions. Potential relationships between the phenolic composition of the extracts determined by LC-ESI-MS/MS and their anti-inflammatory capacity were also evaluated. Hexane and dichloromethane fractions inhibited NOx and IL-12p70 while increased IL-10. Hexane fractions also decreased IL-6 and IFN-γ production. Hexane and dichloromethane fractions showed a higher number of phenolic compounds (32 and 34, respectively) than the other extracts tested and were also the only ones that presented benzoic acid and pinocembrin. These results suggest juçara fruits compounds as potential anti-inflammatory agents, especially those of a more apolar nature.

本研究通过测量一氧化氮(NOx)和细胞因子(IL-12p70、TNF-α、INF-γ、MCP-1、IL-6 和 IL-10),研究了亲水性和亲油性果实(Euterpe edulis Martius)提取物的抗炎作用。用脂多糖(1 µg/mL)刺激 J774 巨噬细胞,并用不同浓度(1-100 µg/mL)的茹切拉果实提取物(包括粗提取物、己烷、二氯甲烷、乙酸乙酯和丁醇馏分)进行处理。此外,还评估了通过 LC-ESI-MS/MS 测定的提取物酚类成分与其抗炎能力之间的潜在关系。正己烷和二氯甲烷馏分抑制了 NOx 和 IL-12p70,同时增加了 IL-10。正己烷馏分还能减少 IL-6 和 IFN-γ 的产生。正己烷和二氯甲烷萃取物显示的酚类化合物数量(分别为 32 和 34)高于其他测试萃取物,也是唯一显示苯甲酸和松果菊酯的萃取物。这些结果表明,Juçara 果实中的化合物具有潜在的抗炎作用,尤其是那些极性较强的化合物。
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引用次数: 0
In Vitro Studies of Pumpkin (Cucurbita moschata var. Kashi Harit) Seed Protein Fraction(s) to Evaluate Anticancer and Antidiabetic Properties. 评估南瓜(Cucurbita moschata var.
IF 3.1 2区 农林科学 Q2 CHEMISTRY, APPLIED Pub Date : 2024-09-01 Epub Date: 2024-07-01 DOI: 10.1007/s11130-024-01205-7
S Vinayashree, C Hemakumar, Ravindra P Veeranna, Ravi Kumar, V Pavithra, V P Mahendra, Prasanna Vasu

Chronic diseases like cancer and diabetes are the major public health concerns of India and worldwide. Nowadays, plant-derived products are in great demand for the treatment of these diseases. Pumpkin seeds are traditionally implicated for their pharmacological properties, as exemplified by benign prostatic hyperplasia. Earlier, pumpkin seed proteins were extracted by the Osborne method, and their functional and nutritional qualities were evaluated. Here, the aim is to assess in vitro, the anticancer and antidiabetic properties of seed protein fractions. HepG2, MDA-MB-231, and MCF-7 cell lines were treated with water-soluble (WF) and alkali-soluble fractions (AF) to assess cytotoxicity, while pancreatic β-cells and insulin resistance (IR) - HepG2 cell lines were treated with WF to evaluate the antidiabetic potential. WF and AF showed cytotoxic effects towards HepG2 and MDA-MB-231 cell lines, suggesting apoptosis-mediated anticancerous activity. WF potentiates glucose-stimulated insulin secretion in pancreatic β-cells, in a dose-dependent manner. In IR-HepG2 cell line studies, control, metformin, and WF-treated groups showed uptake of glucose, when compared to the diabetic group, which is well-correlated with the upregulated expressions of GLUT2 and GLUT4 transporters in these groups. These results indicate that proteins from WF and AF may have anticancerous and antidiabetic properties and thus have the potential to utilize pumpkin proteins in the management of cancer and diabetes.

癌症和糖尿病等慢性疾病是印度乃至全球关注的主要公共卫生问题。如今,治疗这些疾病的植物提取物需求量很大。南瓜籽历来被认为具有药理特性,良性前列腺增生就是一例。早些时候,人们用奥斯本法提取了南瓜籽蛋白质,并对其功能和营养品质进行了评估。本文旨在体外评估南瓜籽蛋白质组分的抗癌和抗糖尿病特性。用水溶性(WF)和碱溶性(AF)馏分处理 HepG2、MDA-MB-231 和 MCF-7 细胞系,以评估细胞毒性;用 WF 处理胰岛β细胞和胰岛素抵抗(IR)- HepG2 细胞系,以评估抗糖尿病潜力。WF 和 AF 对 HepG2 和 MDA-MB-231 细胞株有细胞毒性作用,表明它们具有凋亡介导的抗癌活性。WF 能以剂量依赖的方式增强葡萄糖刺激的胰腺 β 细胞的胰岛素分泌。在IR-HepG2细胞系研究中,与糖尿病组相比,对照组、二甲双胍组和WF处理组都显示出对葡萄糖的吸收,这与这些组中GLUT2和GLUT4转运体的表达上调有很好的相关性。这些结果表明,WF 和 AF 的蛋白质可能具有抗癌和抗糖尿病的特性,因此有可能将南瓜蛋白质用于癌症和糖尿病的治疗。
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引用次数: 0
UPLC-ESI-TQD-MS/MS Identification and Antioxidant, Anti-Inflammatory, Anti-Diabetic, Anti-Obesity and Anticancer Properties of Polyphenolic Compounds of Hawthorn Seeds. 山楂籽多酚类化合物的 UPLC-ESI-TQD-MS/MS 鉴定及其抗氧化、抗炎、抗糖尿病、抗肥胖和抗癌特性。
IF 3.1 2区 农林科学 Q2 CHEMISTRY, APPLIED Pub Date : 2024-09-01 Epub Date: 2024-05-30 DOI: 10.1007/s11130-024-01197-4
Natalia Żurek, Michał Świeca, Ireneusz Kapusta

Hawthorn seeds are a by-product of fruit processing and due to the scale of processing of this raw material, they can be an important source of bioactive compounds. This work is the first report on the phenolic composition of hawthorn seeds and their antioxidant, anti-inflammatory, antidiabetic, antiobesity and anticancer activities. In the isolated phenolic fraction of six seed species, 23 phenolic compounds were identified using the UPLC-ESI-TQD-MS/MS method, the key ones of which included the B-type procyanidin dimer. The seeds of the tested species showed high antioxidant activity (mainly by scavenging O2•- and OH radicals), anti-inflammatory (mainly through LOX inhibition), anti-diabetic, anti-obesity and anti-cancer, with the highest activity against colon cancer cells (Dld-1 line), showing no activity against healthy colon epithelial cells (CCD841CoN). This activity was significantly dependent on the analyzed hawthorn species and, according to PCA analysis, on the content of flavan-3-ols. These discoveries provided the theoretical basis for the possibility of industrial use of hawthorn seeds.

山楂籽是水果加工的副产品,由于这种原材料的加工规模较大,因此可以成为生物活性化合物的重要来源。本研究首次报道了山楂种子的酚类成分及其抗氧化、抗炎、抗糖尿病、抗肥胖和抗癌活性。采用 UPLC-ESI-TQD-MS/MS 方法鉴定了 6 种山楂种子分离出的酚类组分中的 23 种酚类化合物,其中主要包括 B 型原花青素二聚体。被测物种的种子具有很高的抗氧化(主要通过清除 O2 和 OH 自由基)、抗炎(主要通过抑制 LOX)、抗糖尿病、抗肥胖和抗癌活性,其中对结肠癌细胞(Dld-1 株)的活性最高,而对健康的结肠上皮细胞(CCD841CoN)则没有活性。这种活性明显取决于所分析的山楂种类,根据 PCA 分析,还取决于黄烷-3-醇的含量。这些发现为山楂种子的工业应用提供了理论基础。
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引用次数: 0
Inducing Effect of Corylus avellana on Cytotoxic Activity in Lung and Breast Cancer Cells via Apoptosis. 榛子通过细胞凋亡对肺癌和乳腺癌细胞毒性的诱导作用
IF 3.1 2区 农林科学 Q2 CHEMISTRY, APPLIED Pub Date : 2024-09-01 Epub Date: 2024-07-01 DOI: 10.1007/s11130-024-01198-3
Ayşegül Çebi, Yalçın Tepe, İmren Alioglu, Ferda Ari

Turkish hazelnut (Corylus avellana L. cv Tombul) is a widely used nut in the chocolate industry and is also rich in polyphenol content, which promises anticancer effects. The anti-cancer and apoptotic effects of hazelnut leaves extracts examined on lung and breast cancer cells. Sulforhodamine B (SRB) and Adenosine 5'- triphosphate (ATP) assays were carried out for cell viability measurement. The mode of cell death was shown morphologically by the double fluorescence staining. Apoptosis was determined by performing caspase-mediated cytokeratin 18 (M30 ELISA) and western blot analysis. PARP, caspase 3, caspase 8, DR4, and GAPHD (Glyceraldehyde-3-phosphate Dehydrogenase) protein bands were visualized as markers of apoptosis. A wound healing test was employed to measure cell migration. Methanol extract of hazelnut leaf exhibited inhibition of cell growth activities in a dose-dependent manner. IC50 values were determined as 32.17 µg/ml in MCF-7, 32.16 µg/ml in MDA-MB-231, 20.40 µg/ml in A549 and 12.04 µg/ml in H1299 cells for ethanol extract while it was determined as 21.08 µg/ml in MCF-7, 40.16 µg/ml in MDA-MB-231, 22.04 µg/ml in A549 and 5.91 µg/ml in H1299 cells in methanol extract. In comparison, methanol leaf extracts were more effective in H1299 cells (IC50 value was 5.91 µg/ml).In comparison, ethanol leaf extracts were more effective in H1299 cells (IC50 value was 9.722 µg/ml). Western blot analysis demonstrated that hazelnut leaf extract treatment of cancer cells led to cell death via apoptosis and inhibited cell migration in lung and breast cancer cell lines. The cytotoxic effects of hazelnut extract on breast and lung cancer cells might be valuable and promising in elucidating cell death mechanisms for the development of new methods in cancer treatment.

土耳其榛子(Corylus avellana L. cv Tombul)是巧克力工业中广泛使用的一种坚果,也含有丰富的多酚,具有抗癌作用。我们研究了榛子叶提取物对肺癌和乳腺癌细胞的抗癌和凋亡作用。采用磺基多巴胺 B(SRB)和腺苷 5'- 三磷酸(ATP)测定法测量细胞活力。细胞死亡模式通过双荧光染色进行形态学分析。细胞凋亡是通过树突酶介导的细胞角蛋白 18(M30 ELISA)和蛋白印迹分析来确定的。PARP、caspase 3、caspase 8、DR4 和 GAPHD(甘油醛-3-磷酸脱氢酶)蛋白条带作为细胞凋亡的标志物。伤口愈合试验用于测量细胞迁移。榛子叶甲醇提取物对细胞生长活动的抑制作用呈剂量依赖性。乙醇提取物对 MCF-7 细胞、MDA-MB-231 细胞、A549 细胞和 H1299 细胞的 IC50 值分别为 32.17 微克/毫升、32.16 微克/毫升、20.40 微克/毫升和 12.04 微克/毫升,而甲醇提取物对 MCF-7 细胞、MDA-MB-231 细胞、A549 细胞和 H1299 细胞的 IC50 值分别为 21.08 微克/毫升、40.16 微克/毫升、22.04 微克/毫升和 5.91 微克/毫升。相比之下,甲醇叶提取物对 H1299 细胞更有效(IC50 值为 5.91 µg/ml)。Western 印迹分析表明,榛子叶提取物处理癌细胞会导致细胞凋亡,并抑制肺癌和乳腺癌细胞株的细胞迁移。榛子提取物对乳腺癌和肺癌细胞的细胞毒性作用可能对阐明细胞死亡机制、开发癌症治疗新方法具有重要价值和前景。
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引用次数: 0
Apple Pomace Extract Induces Cell Proliferation and Increases Type I Collagen and Hyaluronan Production in Human Skin Fibroblasts In Vitro. 苹果渣提取物可诱导细胞增殖,并增加体外人体皮肤成纤维细胞中 I 型胶原蛋白和透明质酸的生成。
IF 3.1 2区 农林科学 Q2 CHEMISTRY, APPLIED Pub Date : 2024-09-01 Epub Date: 2024-07-13 DOI: 10.1007/s11130-024-01210-w
Naoki Nanashima, Hayato Maeda, Akira Nakajima, Makoto Nishizuka, Tsuyoshi Narumi, Junji Ichita, Koh Itoku

Apple pomace is the residue left after apples are squeezed. The majority of pomace produced worldwide is produced by the apple manufacturing industry, however, most of the pomace produced by the industry is discarded. Apple pomace contains functional ingredients, such as polyphenols and triterpenoids, and exerts several beneficial effects on human health; however, studies on its cosmetic effects on the skin are lacking. Therefore, herein, we investigated the effects of apple pomace extract (APE) on human skin fibroblasts (HSFs) in vitro. When HSFs were cultured with the extract for 72 h, the number of HSFs increased at concentrations of 10 and 20 µg/mL. Transcriptome analysis and reverse transcription-quantitative PCR results revealed that the extract upregulated the expression of hyaluronan synthase (HAS) 1, HAS2, and HAS3 and downregulated the expression of HYAL1, a gene encoding the hyaluronan-degrading enzyme, in HSFs. Additionally, enzyme-linked immunosorbent assay revealed increased amounts of factors related to skin extracellular matrix, such as type I collagen and hyaluronic acid, secreted in the culture supernatant. The western blotting results suggested that the extract induced extracellular signal-regulated kinase and protein kinase B phosphorylation in HSFs. Additionally, several GO_Terms related to mitosis were detected in the Gene Ontology analysis. This is the first study to show that APE induces the proliferation of HSFs and production of factors related to skin anti-aging.

苹果渣是苹果榨汁后留下的残渣。全世界生产的大部分果渣由苹果制造业生产,但该行业生产的大部分果渣都被丢弃。苹果渣含有多酚和三萜类化合物等功能性成分,对人体健康有多种益处,但缺乏对其皮肤美容效果的研究。因此,我们在本文中研究了苹果渣提取物(APE)对人体皮肤成纤维细胞(HSFs)的体外影响。用萃取物培养成纤维细胞 72 小时后,浓度为 10 和 20 µg/mL 的成纤维细胞数量有所增加。转录组分析和反转录定量 PCR 结果显示,提取物能上调 HSF 中透明质酸合成酶(HAS)1、HAS2 和 HAS3 的表达,并下调 HYAL1(一种编码透明质酸降解酶的基因)的表达。此外,酶联免疫吸附试验显示,培养上清液中与皮肤细胞外基质有关的因子,如 I 型胶原和透明质酸的分泌量增加。Western印迹结果表明,该提取物可诱导 HSFs 中的细胞外信号调节激酶和蛋白激酶 B 磷酸化。此外,在基因本体分析中还发现了几个与有丝分裂相关的GO_Terms。这是首次研究表明 APE 能诱导 HSFs 增殖并产生与皮肤抗衰老相关的因子。
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引用次数: 0
Sesamol Alleviates Sarcopenia via Activating AKT/mTOR/FoxO1 Signal Pathway in Aged Obese Mice. 芝麻酚通过激活 AKT/mTOR/FoxO1 信号通路缓解老年肥胖症小鼠的肌肉疏松症
IF 3.1 2区 农林科学 Q2 CHEMISTRY, APPLIED Pub Date : 2024-09-01 Epub Date: 2024-06-15 DOI: 10.1007/s11130-024-01199-2
Jinxin Yang, Zhipeng Wang, Yan Xie, Yongyan Tang, Yansong Fu, Zhuoya Xu, Jingmiao Chen, Hong Qin

Sesamol is a major bioactive component extracted from sesame seeds and has various medicinal properties. However, the effects of sesamol on sarcopenia associated with aging and obesity remains unclear. Therefore, the protective effects and underlying mechanisms of sesamol on sarcopenia was evaluated in aged and obese C57BL/6 J male mouse models fed a high fat diet and C2C12 myotubes co-treated with D-gal and PA in this study. Our in vivo data showed that sesamol activated AKT/mTOR/FoxO1 signal pathway, and then upregulated p-p70S6K and p-4EBP1 to promote myoprotein synthesis, and downregulated Atrogin-1 and MuRF1 to inhibit myoprotein degradation, thus ameliorating sarcopenia related to aging and obesity. Furthermore, our in vitro results confirmed the protective effect and aforementioned mechanisms of sesamol on sarcopenia. Collectively, sesamol could alleviate sarcopenia associated with aging and obesity via activating the AKT/mTOR/FoxO1 signal pathway. Our findings highlight the therapeutic potentials of sesamol for aging and obesity-related metabolic muscular complications.

芝麻酚是从芝麻中提取的一种主要生物活性成分,具有多种药用功效。然而,芝麻酚对与衰老和肥胖相关的肌肉疏松症的影响仍不清楚。因此,本研究评估了芝麻酚对以高脂饮食喂养的老年肥胖 C57BL/6 J 雄性小鼠模型和以 D-gal 和 PA 联合处理的 C2C12 肌细胞管肌肉疏松症的保护作用及其内在机制。体内数据显示,芝麻酚可激活 AKT/mTOR/FoxO1 信号通路,进而上调 p-p70S6K 和 p-4EBP1 以促进肌蛋白合成,下调 Atrogin-1 和 MuRF1 以抑制肌蛋白降解,从而改善与衰老和肥胖相关的肌肉疏松症。此外,我们的体外研究结果证实了芝麻酚对肌肉疏松症的保护作用和上述机制。总之,芝麻酚可通过激活AKT/mTOR/FoxO1信号通路,缓解与衰老和肥胖相关的肌肉疏松症。我们的研究结果凸显了芝麻酚对衰老和肥胖相关的代谢性肌肉并发症的治疗潜力。
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引用次数: 0
Jackfruit Leaf Protein Hydrolysates Obtained by Enzymatic Hydrolysis of Leaf Protein Concentrate with Pepsin and Pancreatin: Molecular Weight, Cytotoxicity, Antiproliferative Activity, and Oxidative Stress. 用胃蛋白酶和胰蛋白酶酶解浓缩叶蛋白获得的菠萝叶蛋白水解物:分子量、细胞毒性、抗增殖活性和氧化应激。
IF 3.1 2区 农林科学 Q2 CHEMISTRY, APPLIED Pub Date : 2024-09-01 Epub Date: 2024-07-10 DOI: 10.1007/s11130-024-01203-9
Carolina Calderón-Chiu, Juan Arturo Ragazzo-Sánchez, Armando Ordaz-Hernández, Mayra Herrera-Martínez

Jackfruit leaf protein hydrolysates obtained from the enzymatic hydrolysis of leaf protein concentrate with gastrointestinal enzymes have shown good techno-functional properties and high antioxidant capacity. However, molecular weight, antiproliferative activity, cytotoxicity and the ability to reduce reactive oxygen species (ROS) are still unknown. Therefore, this study aimed to evaluate the effect of jackfruit leaf protein hydrolysates obtained by enzymatic hydrolysis with pepsin and pancreatin at different hydrolysis times (30-240 min) on molecular weights, cytotoxicity, antiproliferation of cancer cells, and the reduction of reactive oxygen species in H2O2-induced HaCaT cells. The electrophoretic profile indicated that H-Pep contains peptides with molecular weights between 25 - 20 kDa. Meanwhile, H-Pan is composed of molecular weight products between 25 - 20 kDa and < 20 kDa. H-Pan and H-Pep (125-500 µg/mL) did not show significant cytotoxicity on HaCaT (human keratinocytes) and J774A.1 (murine macrophage cells). Antiproliferative activity was achieved in human cervical, ovarian, and liver cancer cells. H-Pan-240 min (1000 µg/mL) reduced the cell viability of cervical cancer cells by 23% while H-Pan-60 min significantly reduced cell viability of ovarian and liver cancer cells by 14.5 (500 µg/mL) and 17% (1000 µg/mL), respectively (P < 0.05). The protective effect against oxidative stress on H2O2-stressed HaCaT cells was obtained with H-Pep-60 min, which reduced 25% of ROS at 250 µg/mL (P < 0.05). The findings demonstrate the safe use of green biomass as a source of plant protein hydrolysates.

用胃肠道酶对浓缩叶蛋白进行酶水解得到的菠萝叶蛋白水解物显示出良好的技术功能特性和较高的抗氧化能力。然而,其分子量、抗增殖活性、细胞毒性和减少活性氧(ROS)的能力仍是未知数。因此,本研究旨在评估在不同水解时间(30-240 分钟)下用胃蛋白酶和胰蛋白酶进行酶水解得到的菠萝叶蛋白水解物对分子量、细胞毒性、抗癌细胞增殖以及减少 H2O2- 诱导的 HaCaT 细胞中活性氧的影响。电泳图谱显示,H-Pep 含有分子量在 25 - 20 kDa 之间的肽。同时,H-Pan 是由分子量在 25 - 20 kDa 之间的产物组成,H-Pep-60 分钟可使 2O2 受激的 HaCaT 细胞在 250 µg/mL 浓度下减少 25% 的 ROS(P
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引用次数: 0
Differential Efficacy of Storage Temperature and Postharvest Treatment on Shelf Life and Quality of Avocado Fruit (Persea americana Mill.). 贮藏温度和采后处理剂对牛油果(Persea americana Mill.)保质期和质量的不同功效。
IF 3.1 2区 农林科学 Q2 CHEMISTRY, APPLIED Pub Date : 2024-09-01 Epub Date: 2024-07-06 DOI: 10.1007/s11130-024-01207-5
Vittal Kamble, C K Narayana, G Karunakaran, D V Sudhakar Rao, S Sriram, Laxman R H

Avocado fruit is a rich source of phytonutrients such as vitamins, minerals, carotenoids, carbohydrates, polyphenols and unsaturated fatty acids. However, due to its climacteric nature, fruits are highly susceptible to storage temperature, resulting in poor shelf life and reduced quality. In the present study avocado fruits (Accession CHES-HA-I/I) were stored at different low temperatures (5, 9 and 12 °C with 90-95% relative humidity, RH) to identify optimum low temperature for cold storage. In a further experiment, avocado fruits were treated with 1-methylcyclopropene (1-MCP, 500 ppb) and chitosan (0.5%) to extend the shelf life with better fruit quality. The results showed that storage temperatures had significant effect on physiological, biochemical and antioxidant activities of fruits. Lower physiological loss in weight (PLW), reduced respiration and ethylene production, and higher carbohydrates, protein and fat content were recorded in fruits stored at 9 °C as compared to 12 °C. Similarly, maximum antioxidant properties in terms of free radical scavenging activity (FRSA) and ferric reducing ability of plasma (FRAP) was found in avocado fruits stored at 9 °C. It was also noticed that chilling injury was developed in fruits stored under 5 °C. In addition, exogenous application of 1-MCP significantly reduced respiration and ethylene production rate at 9 °C and extended the shelf life up to 42 days with better fruit quality and more antioxidant activities. However, chitosan treated and control fruits had shelf life up to 28 and 21 days respectively, with minimum nutritional content. From this study it is concluded that a storage temperature of 9 °C and 1-MCP treatment significantly enhanced the shelf life of avocado fruits with better fruit quality as compared to other storage temperatures (5 and 12 °C) and postharvest treatment (chitosan).

牛油果含有丰富的植物营养素,如维生素、矿物质、类胡萝卜素、碳水化合物、多酚和不饱和脂肪酸。然而,由于其气候性状,水果极易受贮藏温度的影响,导致货架期短和质量下降。在本研究中,牛油果果实(登录号 CHES-HA-I/I)在不同的低温(5、9 和 12 °C,相对湿度 90-95%)下贮藏,以确定冷藏的最佳低温。在另一项实验中,用 1-甲基环丙烯(1-MCP,500 ppb)和壳聚糖(0.5%)处理鳄梨果实,以延长货架期,提高果实质量。结果表明,贮藏温度对水果的生理、生化和抗氧化活性有显著影响。与 12 °C 相比,9 °C 下贮藏的水果生理失重(PLW)更低,呼吸作用和乙烯产生减少,碳水化合物、蛋白质和脂肪含量更高。同样,在自由基清除活性(FRSA)和血浆铁还原能力(FRAP)方面,9 °C 下贮藏的牛油果具有最高的抗氧化特性。同时还发现,在 5 °C 下贮藏的果实会出现冷冻损伤。此外,外源施用 1-MCP 能显著降低 9 °C 下的呼吸作用和乙烯产生率,并能延长货架期至 42 天,同时提高果实质量和抗氧化活性。然而,经壳聚糖处理的水果和对照水果的货架期分别为 28 天和 21 天,营养成分含量最低。这项研究得出的结论是,与其他储存温度(5 和 12 °C)和采后处理(壳聚糖)相比,9 °C 的储存温度和 1-MCP 处理能显著延长牛油果水果的货架期,提高水果质量。
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引用次数: 0
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Plant Foods for Human Nutrition
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