Adil Laaziz, Yousra EL HAMMOUDI, Souad Qjidaa, A. Hajjaji, H. Hajjaj, G. Haesaert, A. Bouseta
Essential oils have been used since ancient times in traditional medicine and agri-food science to preserve food, and to combat human diseases. Essential oils (EOs) from clove and rosemary, obtained by hydro-distillation, were analyzed by GC/MS and evaluated for their antifungal activity against strains of Aspergillus tubingensis and A. luchuensis and their effect on ochratoxin A (OTA) production by these fungi. The major constituent of clove EO was eugenol (86.4%), and of rosemary EO was eucalyptol (35.9%). Mycelium growth inhibition assays showed that the EOs had dose-depend inhibition effects, which reached 36.6% for rosemary EO, and 100% for clove EO, at EO concentration of 200 μL L-1. These reductions were different for different Aspergillus strains, the essential oil type, and the strain/oil type interaction. Analyses of OTA in the culture medium extracts of Aspergillus strains was carried out using HPLC-FLD, and was confirmed by LC-MS/MS for positive controls of two OTA-producing strains of A. tubingensis. In general, OTA was reduced (from 45 to 100% reduction), except for two strains of A. tubingensis, where OTA production was stimulated.
{"title":"Activity of essential oils from Syzygium aromaticum and Rosmarinus officinalis against growth and ochratoxin A production by Aspergillus tubingensis and Aspergillus luchuensis from Moroccan grapes","authors":"Adil Laaziz, Yousra EL HAMMOUDI, Souad Qjidaa, A. Hajjaji, H. Hajjaj, G. Haesaert, A. Bouseta","doi":"10.36253/phyto-12841","DOIUrl":"https://doi.org/10.36253/phyto-12841","url":null,"abstract":"Essential oils have been used since ancient times in traditional medicine and agri-food science to preserve food, and to combat human diseases. Essential oils (EOs) from clove and rosemary, obtained by hydro-distillation, were analyzed by GC/MS and evaluated for their antifungal activity against strains of Aspergillus tubingensis and A. luchuensis and their effect on ochratoxin A (OTA) production by these fungi. The major constituent of clove EO was eugenol (86.4%), and of rosemary EO was eucalyptol (35.9%). Mycelium growth inhibition assays showed that the EOs had dose-depend inhibition effects, which reached 36.6% for rosemary EO, and 100% for clove EO, at EO concentration of 200 μL L-1. These reductions were different for different Aspergillus strains, the essential oil type, and the strain/oil type interaction. Analyses of OTA in the culture medium extracts of Aspergillus strains was carried out using HPLC-FLD, and was confirmed by LC-MS/MS for positive controls of two OTA-producing strains of A. tubingensis. In general, OTA was reduced (from 45 to 100% reduction), except for two strains of A. tubingensis, where OTA production was stimulated.","PeriodicalId":20165,"journal":{"name":"Phytopathologia Mediterranea","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2022-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43324481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Giovanni Del Frari, F. Calzarano, Ricardo Boavida Ferreira
A peculiar symptom that may develop in grapevines affected by wood pathogens involved in the esca complex of diseases is the leaf stripe symptom, which also gives the name to the Grapevine Leaf Stripe Disease. Multiple studies have revealed strong links between fungal presence, wood symptomatology and expression of the leaf stripe symptom. However, numerous other factors have been shown to play roles in symptom onset, incidence, severity and yearly fluctuation of this disease. While the factors triggering the leaf stripe symptom are still under investigation, three control strategies have been proven effective for substantially reducing its expression, namely trunk surgery, and applications of sodium arsenite or a fertilizer mixture. These control strategies are examined here, including their (putative or confirmed) modes of action, and how they may influence the leaf stripe symptom development. In this article, we also propose the ‘edge’ hypothesis to tentatively explain symptoms onset, keeping in consideration past knowledge and recent advances in the understanding of the esca leaf stripe symptom. Ultimately, it is our intention to offer food-for-thought and stimulate debate within the phytopathological community.
{"title":"Understanding the control strategies effective against the esca leaf stripe symptom: the edge hypothesis","authors":"Giovanni Del Frari, F. Calzarano, Ricardo Boavida Ferreira","doi":"10.36253/phyto-13295","DOIUrl":"https://doi.org/10.36253/phyto-13295","url":null,"abstract":"A peculiar symptom that may develop in grapevines affected by wood pathogens involved in the esca complex of diseases is the leaf stripe symptom, which also gives the name to the Grapevine Leaf Stripe Disease. Multiple studies have revealed strong links between fungal presence, wood symptomatology and expression of the leaf stripe symptom. However, numerous other factors have been shown to play roles in symptom onset, incidence, severity and yearly fluctuation of this disease. While the factors triggering the leaf stripe symptom are still under investigation, three control strategies have been proven effective for substantially reducing its expression, namely trunk surgery, and applications of sodium arsenite or a fertilizer mixture. These control strategies are examined here, including their (putative or confirmed) modes of action, and how they may influence the leaf stripe symptom development. In this article, we also propose the ‘edge’ hypothesis to tentatively explain symptoms onset, keeping in consideration past knowledge and recent advances in the understanding of the esca leaf stripe symptom. Ultimately, it is our intention to offer food-for-thought and stimulate debate within the phytopathological community.","PeriodicalId":20165,"journal":{"name":"Phytopathologia Mediterranea","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2022-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46237305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The 16th Congress of the Mediterranean Phytopathological Union (MPU2022) took place in Limassol, Cyprus from 4–8 April, 2022. Plant pathologists from the region and beyond celebrated the 60th anniversary of the Union and the 62 years of publication of Phytopathologia Mediterranea as a top level plant pathology journal. MPU2022 entitled “Safeguarding Mediterranean Plant Health” promoted dissemination of the latest scientific advances, and facilitated dialogue and collaboration between researchers interested in all aspects of Phytopathology. This conference also addressed wider perspectives in scientific fields not previously explored at MPU Congresses, including abiotic stresses, biopesticides, forest pathology, smart agriculture and gender equality in research, funding, teaching and professional development. Papers presented at the Congress included 83 oral presentations, including 15 keynote presentations from leading scientists, and 63 poster presentations, covering all areas of plant pathology and plant stress research, from 130 participants. Five special sessions in cutting edge subjects were organised. A special session was organised jointly with the Arab Society of Plant Protection. All these scientific contributions are part of this issue.
{"title":"Abstracts of invited, oral and poster papers presented at the 16th Congress of the Mediterranean Phytopathological Union, April 4–8, 2022, in Limassol, Cyprus","authors":"L. Mugnai","doi":"10.36253/phyto-13613","DOIUrl":"https://doi.org/10.36253/phyto-13613","url":null,"abstract":"The 16th Congress of the Mediterranean Phytopathological Union (MPU2022) took place in Limassol, Cyprus from 4–8 April, 2022. Plant pathologists from the region and beyond celebrated the 60th anniversary of the Union and the 62 years of publication of Phytopathologia Mediterranea as a top level plant pathology journal. \u0000MPU2022 entitled “Safeguarding Mediterranean Plant Health” promoted dissemination of the latest scientific advances, and facilitated dialogue and collaboration between researchers interested in all aspects of Phytopathology. This conference also addressed wider perspectives in scientific fields not previously explored at MPU Congresses, including abiotic stresses, biopesticides, forest pathology, smart agriculture and gender equality in research, funding, teaching and professional development. \u0000Papers presented at the Congress included 83 oral presentations, including 15 keynote presentations from leading scientists, and 63 poster presentations, covering all areas of plant pathology and plant stress research, from 130 participants. Five special sessions in cutting edge subjects were organised. A special session was organised jointly with the Arab Society of Plant Protection. All these scientific contributions are part of this issue.","PeriodicalId":20165,"journal":{"name":"Phytopathologia Mediterranea","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2022-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45487697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Podosphaera xanthii causes powdery mildew of cucumber, and is associated with significant yield and quality losses. Development of resistant or tolerant varieties is the most effective and eco-friendly strategy for powdery mildew management. An important host resistance mechanism is based on the recognition of conserved resistance genes, resulting in durable resistance. To determine powdery mildew resistance mechanisms in cucumber, total RNAs were isolated from the powdery mildew resistant cultivar Meltem, the tolerant line VT18, and the susceptible local variety Camlica. Expression levels of nine genes in these plants were analysed by Reverse Transcription Polymerase Chain Reaction (RT-PCR). The host reactions were assessed using microscope observations of stained specimens. Serine/threonine (STN7), transcription factor (WRKY22), serine/threonine-protein kinase (D6PKL1), and serine/threonine receptor kinase (NFP) genes were induced, as positive regulators in defence mechanisms against powdery mildew. Polygalacturonase Inhibitor (PGIP) did not express after P. xanthii inoculation of Camlica, resulting in susceptibility. After inoculation, callose synthase (CALLOSE) and cinnamyl alcohol dehydrogenase (CAD) gene expression levels were increased in resistant Meltem, but Hypersensitive Reaction (HR) and ROS formation were only linked in the tolerant VT18. Powdery mildew development was less in Meltem than in VT18, indicating that cell wall thickening and HR play separate roles in resistance to this disease.
{"title":"Mechanisms of resistance to powdery mildew in cucumber","authors":"Mumin Ibrahim Tek, O. Calis","doi":"10.36253/phyto-13313","DOIUrl":"https://doi.org/10.36253/phyto-13313","url":null,"abstract":"Podosphaera xanthii causes powdery mildew of cucumber, and is associated with significant yield and quality losses. Development of resistant or tolerant varieties is the most effective and eco-friendly strategy for powdery mildew management. An important host resistance mechanism is based on the recognition of conserved resistance genes, resulting in durable resistance. To determine powdery mildew resistance mechanisms in cucumber, total RNAs were isolated from the powdery mildew resistant cultivar Meltem, the tolerant line VT18, and the susceptible local variety Camlica. Expression levels of nine genes in these plants were analysed by Reverse Transcription Polymerase Chain Reaction (RT-PCR). The host reactions were assessed using microscope observations of stained specimens. Serine/threonine (STN7), transcription factor (WRKY22), serine/threonine-protein kinase (D6PKL1), and serine/threonine receptor kinase (NFP) genes were induced, as positive regulators in defence mechanisms against powdery mildew. Polygalacturonase Inhibitor (PGIP) did not express after P. xanthii inoculation of Camlica, resulting in susceptibility. After inoculation, callose synthase (CALLOSE) and cinnamyl alcohol dehydrogenase (CAD) gene expression levels were increased in resistant Meltem, but Hypersensitive Reaction (HR) and ROS formation were only linked in the tolerant VT18. Powdery mildew development was less in Meltem than in VT18, indicating that cell wall thickening and HR play separate roles in resistance to this disease.","PeriodicalId":20165,"journal":{"name":"Phytopathologia Mediterranea","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2022-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45495626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Copper is an essential element for microbes as it is involved in many redox reactions. Numerous resistance systems have been evolved in microbes to maintain copper homeostasis under copper stress conditions. These systems are responsible for the influx and efflux of copper ions in the cells. In phytopathogenic bacteria, copper ions play essential roles during disease development in plants. Copper-based chemicals are extensively used for control of diseases caused by bacteria, which leads to induced pathogen resistance derived from various copper resistance systems. Previous studies have shown that copper ions are harnessed by host plants to protect against bacterial infections, triggering immune responses through activation of defence signalling pathways. Thus, it was anticipated that bacterial copper resistance could play an alternative role in adaptation to plant immunity. This review summarizes current knowledge of copper resistance systems in plant pathogenic bacteria, which may provide a new perspective of molecular mechanisms associated with bacterial adaptation in host plants.
{"title":"Copper resistance mechanisms in plant pathogenic bacteria","authors":"Xiaojing Fan, T. Saleem, Huasong Zou","doi":"10.36253/phyto-13282","DOIUrl":"https://doi.org/10.36253/phyto-13282","url":null,"abstract":"Copper is an essential element for microbes as it is involved in many redox reactions. Numerous resistance systems have been evolved in microbes to maintain copper homeostasis under copper stress conditions. These systems are responsible for the influx and efflux of copper ions in the cells. In phytopathogenic bacteria, copper ions play essential roles during disease development in plants. Copper-based chemicals are extensively used for control of diseases caused by bacteria, which leads to induced pathogen resistance derived from various copper resistance systems. Previous studies have shown that copper ions are harnessed by host plants to protect against bacterial infections, triggering immune responses through activation of defence signalling pathways. Thus, it was anticipated that bacterial copper resistance could play an alternative role in adaptation to plant immunity. This review summarizes current knowledge of copper resistance systems in plant pathogenic bacteria, which may provide a new perspective of molecular mechanisms associated with bacterial adaptation in host plants.","PeriodicalId":20165,"journal":{"name":"Phytopathologia Mediterranea","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2022-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46973552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Licciardello, J. Moral, M. C. Strano, P. Caruso, M. Sciara, P. Bella, G. Sorrentino, S. Di Silvestro
Anthracnose caused by Colletotrichum spp. is the most damaging olive fruit disease in many countries, including Italy. This disease has been sporadically detected in Sicily, but new agronomic practices can increase risk of olive anthracnose in this region. An etiological study of the disease focused on local olive cultivars growing at the International Olive Germplasm Collection (IOGC) in Villa Zagaria, Enna, Sicily has been undertaken. During 2018 and 2019, 137 Colletotrichum strains were isolated from olives. Colony morphology, conidium characteristics, and multilocus sequence analyses aided identification of three species: C. acutatum (affecting 70% of symptomatic olives), C. gloeosporioides, and C. cigarro. Three C. acutatum strains (B13-16, P77, and P185), and one strain of each C. gloeosporioides (C2.1) and C. cigarro (Perg6B) were evaluated for pathogenicity on olive fruits from 11 Sicilian cultivars, known for their high-quality oil. Differences in virulence were detected among strains and their pathogenicity to the cultivars. The C. acutatum isolates were more virulent than those of C. gloeosporioides or C. cigarro. The Sicilian olive cultivars Cavaliera, Carolea, Calatina, and Nocellara del Belice were the most susceptible to the pathogen, while the cultivars Biancolilla and Nocellara Etnea were the most tolerant. Cultivar response under field conditions showed that anthracnose severity and fruit-rot incidence were positively correlated. This is the first report of C. acutatum and C. cigarro affecting olive trees in Sicily. Control measures for anthracnose depend on accurate characterization of the etiological agents and host cultivar resistance.
炭疽病是包括意大利在内的许多国家最具破坏性的橄榄果实疾病。这种疾病在西西里岛偶尔被发现，但新的农艺措施会增加该地区橄榄炭疽病的风险。在西西里岛恩纳的Villa Zagaria的国际橄榄种质资源收藏馆（IOGC），对当地橄榄品种进行了该疾病的病原学研究。2018年至2019年间，从橄榄中分离出137株炭疽菌。菌落形态、分生孢子特征和多点序列分析有助于鉴定三个物种：尖尖尖橄榄（影响70%有症状的橄榄）、球孢C.gloeosporioides和cigarro。对三株尖尖尖孢C.acutatum菌株（B13-16、P77和P185）以及球孢C.gloeosporioides（C2.1）和cigarro（Perg6B）各一株菌株对11个西西里品种的橄榄果实的致病性进行了评估，这些品种以其高质量的油而闻名。菌株之间的毒力及其对品种的致病性存在差异。尖孢C.acutatum分离株的毒力强于球孢C.gloeosporioides或cigarro分离株。西西里橄榄品种Cavaliera、Carolea、Calatina和Nocellara del Belice对病原体最敏感，而品种Biancolilla和Nocellera Etnea对病原体最耐受。田间试验结果表明，炭疽病的严重程度与果实腐烂病的发生率呈正相关。这是第一份关于C.acutatum和C.cigarro影响西西里岛橄榄树的报告。炭疽病的控制措施取决于病原体和寄主品种抗性的准确鉴定。
{"title":"Characterization of Colletotrichum strains associated with olive anthracnose in Sicily","authors":"G. Licciardello, J. Moral, M. C. Strano, P. Caruso, M. Sciara, P. Bella, G. Sorrentino, S. Di Silvestro","doi":"10.36253/phyto-13181","DOIUrl":"https://doi.org/10.36253/phyto-13181","url":null,"abstract":"Anthracnose caused by Colletotrichum spp. is the most damaging olive fruit disease in many countries, including Italy. This disease has been sporadically detected in Sicily, but new agronomic practices can increase risk of olive anthracnose in this region. An etiological study of the disease focused on local olive cultivars growing at the International Olive Germplasm Collection (IOGC) in Villa Zagaria, Enna, Sicily has been undertaken. During 2018 and 2019, 137 Colletotrichum strains were isolated from olives. Colony morphology, conidium characteristics, and multilocus sequence analyses aided identification of three species: C. acutatum (affecting 70% of symptomatic olives), C. gloeosporioides, and C. cigarro. Three C. acutatum strains (B13-16, P77, and P185), and one strain of each C. gloeosporioides (C2.1) and C. cigarro (Perg6B) were evaluated for pathogenicity on olive fruits from 11 Sicilian cultivars, known for their high-quality oil. Differences in virulence were detected among strains and their pathogenicity to the cultivars. The C. acutatum isolates were more virulent than those of C. gloeosporioides or C. cigarro. The Sicilian olive cultivars Cavaliera, Carolea, Calatina, and Nocellara del Belice were the most susceptible to the pathogen, while the cultivars Biancolilla and Nocellara Etnea were the most tolerant. Cultivar response under field conditions showed that anthracnose severity and fruit-rot incidence were positively correlated. This is the first report of C. acutatum and C. cigarro affecting olive trees in Sicily. Control measures for anthracnose depend on accurate characterization of the etiological agents and host cultivar resistance.","PeriodicalId":20165,"journal":{"name":"Phytopathologia Mediterranea","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2022-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43921820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. GERIČ STARE, Nikola Šušić, Maja Starovič, S. Širca
The tropical root-knot nematode (RKN) Meloidogyne inornata infects soybean, tobacco, yacon, common bean and Anthurium andreanum. This species was reported as the cause of damage in commercial yacon production in Brazil, and has potential to cause losses to common bean crops. Potato (Solanum tuberosum L.) cv. Désirée infected with M. inornata exhibited typical RKN galls on roots and tuber surfaces, similar to those caused by the two quarantine pathogens M. chitwoodi and M. fallax, and the non-quarantine M. luci. This study has indicated that M. inornata has considerable potential to cause severe damage to potato tubers. The potential spread of this pathogen into new areas should be assessed, as it can damage potato tubers and could be a problem for economically important crops. Ensuring pest-free seed potatoes is important to prevent dissemination and establishment of M. inornata in uninfested areas. Phytosanitary measures and monitoring programmes developed to prevent spread of this pest in Europe may be warranted.
{"title":"Potato (Solanum tuberosum) - a new host for the root-knot nematode Meloidogyne inornata","authors":"B. GERIČ STARE, Nikola Šušić, Maja Starovič, S. Širca","doi":"10.36253/phyto-13355","DOIUrl":"https://doi.org/10.36253/phyto-13355","url":null,"abstract":"The tropical root-knot nematode (RKN) Meloidogyne inornata infects soybean, tobacco, yacon, common bean and Anthurium andreanum. This species was reported as the cause of damage in commercial yacon production in Brazil, and has potential to cause losses to common bean crops. Potato (Solanum tuberosum L.) cv. Désirée infected with M. inornata exhibited typical RKN galls on roots and tuber surfaces, similar to those caused by the two quarantine pathogens M. chitwoodi and M. fallax, and the non-quarantine M. luci. This study has indicated that M. inornata has considerable potential to cause severe damage to potato tubers. The potential spread of this pathogen into new areas should be assessed, as it can damage potato tubers and could be a problem for economically important crops. Ensuring pest-free seed potatoes is important to prevent dissemination and establishment of M. inornata in uninfested areas. Phytosanitary measures and monitoring programmes developed to prevent spread of this pest in Europe may be warranted.","PeriodicalId":20165,"journal":{"name":"Phytopathologia Mediterranea","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2022-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41868003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Maleita, António Correia, I. Abrantes, I. Esteves
The root-knot nematode (RKN) Meloidogyne luci is included in the Alert List of the European Plant Protection Organization, because it has potential negative impacts on economically important crops. Identification of plant species/cultivars resistant to M. luci is important for its management. Susceptibility of 35 commercial plant species/cultivars, from nine families to a M. luci isolate from Portugal was evaluated in pot assays, assessing root gall index (GI) and reproduction factor (Rf) 60 d after inoculation, with tomato ‘Coração-de-Boi’ used as the positive susceptible experimental control. Presence/absence of RKN resistance genes was also determined in the tomato and pepper cultivars. One cultivar of cabbage, three of lettuce, ten of pepper, one of sugar beet, and all the cultivars of Cucurbitaceae (five), Fabaceae (two) and Poaceae (one) were susceptible to M. luci (GI = 4-5; Rf = 2.1-152.3). One cultivar each of carrot, passion fruit, lettuce ‘Cocktail’, cabbage ‘Bacalan’, ‘Coração’ and ‘Lombarda’, and spinach ‘Tayto’ were resistant/hypersensitive (Rf < 1; GI > 2). The tomato ‘Actimino’, ‘Briomino’, ‘Veinal’ and ‘Vimeiro’, which carried at least one copy of the Mi-1.2 gene, were resistant to the nematode (GI = 1-2; 0.0 < Rf < 0.1). These results indicate that the tomato cultivars have potential to contribute to reduction of M. luci populations in agro-ecosystems and improve the crop yields.
{"title":"Susceptibility of crop plants to the root-knot nematode Meloidogyne luci, a threat to agricultural productivity","authors":"C. Maleita, António Correia, I. Abrantes, I. Esteves","doi":"10.36253/phyto-13369","DOIUrl":"https://doi.org/10.36253/phyto-13369","url":null,"abstract":"The root-knot nematode (RKN) Meloidogyne luci is included in the Alert List of the European Plant Protection Organization, because it has potential negative impacts on economically important crops. Identification of plant species/cultivars resistant to M. luci is important for its management. Susceptibility of 35 commercial plant species/cultivars, from nine families to a M. luci isolate from Portugal was evaluated in pot assays, assessing root gall index (GI) and reproduction factor (Rf) 60 d after inoculation, with tomato ‘Coração-de-Boi’ used as the positive susceptible experimental control. Presence/absence of RKN resistance genes was also determined in the tomato and pepper cultivars. One cultivar of cabbage, three of lettuce, ten of pepper, one of sugar beet, and all the cultivars of Cucurbitaceae (five), Fabaceae (two) and Poaceae (one) were susceptible to M. luci (GI = 4-5; Rf = 2.1-152.3). One cultivar each of carrot, passion fruit, lettuce ‘Cocktail’, cabbage ‘Bacalan’, ‘Coração’ and ‘Lombarda’, and spinach ‘Tayto’ were resistant/hypersensitive (Rf < 1; GI > 2). The tomato ‘Actimino’, ‘Briomino’, ‘Veinal’ and ‘Vimeiro’, which carried at least one copy of the Mi-1.2 gene, were resistant to the nematode (GI = 1-2; 0.0 < Rf < 0.1). These results indicate that the tomato cultivars have potential to contribute to reduction of M. luci populations in agro-ecosystems and improve the crop yields.","PeriodicalId":20165,"journal":{"name":"Phytopathologia Mediterranea","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2022-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42849951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Guanxiu Guan, Shu Liu, U. Braun, Penglei Qiu, Jian Liu, F. Zhao, Shu‐Rong Tang, Jia-Ni Li, Van-Ninh Nguyen
The Golovinomyces cichoracearum complex is morphologically highly variable and causes powdery mildew on a wide range of Compositae, including Hieracium spp. and Lapsana communis. A comprehensive phylogenetic analysis of Golovinomyces, published in 2013, revealed that G. cichoracearum s. str. was confined to Scorzonera and Tragopogon spp. as hosts (Compositae, tribe Lactuceae, subtribe Scorzonerinae). To clarify the phylogeny and taxonomy of Golovinomyces on Hieracium spp. (Compositae, tribe Lactuceae, subtribe Hieraciinae), which is common in Asia and Europe, morphology and phylogenetic analyses of ITS + 28S rDNA sequences were conducted for 57 powdery mildew specimens of Hieracium spp. and Lapsana communis. Golovinomyces on Hieracium spp. in Asia and Europe, previously referred to as G. cichoracearum, is a previously undescribed, cryptic species, which is described here as Golovinomyces hieraciorum sp. nov.. Since a sequence retrieved from a powdery mildew on Lapsana communis in Switzerland clustered in sister position to the G. hieraciorum clade, numerous German specimens identified as G. cichoracearum were included in the present examinations. All the re-examined collections of “G. cichoracearum” on L. communis were misidentified, and were shown to belong to Neoërysiphe nevoi or Podosphaera ergerontis-canadensis.
{"title":"A cryptic powdery mildew (Golovinomyces hieraciorum sp. nov.) on Hieracium and Pilosella (Compositae)","authors":"Guanxiu Guan, Shu Liu, U. Braun, Penglei Qiu, Jian Liu, F. Zhao, Shu‐Rong Tang, Jia-Ni Li, Van-Ninh Nguyen","doi":"10.36253/phyto-12992","DOIUrl":"https://doi.org/10.36253/phyto-12992","url":null,"abstract":"The Golovinomyces cichoracearum complex is morphologically highly variable and causes powdery mildew on a wide range of Compositae, including Hieracium spp. and Lapsana communis. A comprehensive phylogenetic analysis of Golovinomyces, published in 2013, revealed that G. cichoracearum s. str. was confined to Scorzonera and Tragopogon spp. as hosts (Compositae, tribe Lactuceae, subtribe Scorzonerinae). To clarify the phylogeny and taxonomy of Golovinomyces on Hieracium spp. (Compositae, tribe Lactuceae, subtribe Hieraciinae), which is common in Asia and Europe, morphology and phylogenetic analyses of ITS + 28S rDNA sequences were conducted for 57 powdery mildew specimens of Hieracium spp. and Lapsana communis. Golovinomyces on Hieracium spp. in Asia and Europe, previously referred to as G. cichoracearum, is a previously undescribed, cryptic species, which is described here as Golovinomyces hieraciorum sp. nov.. Since a sequence retrieved from a powdery mildew on Lapsana communis in Switzerland clustered in sister position to the G. hieraciorum clade, numerous German specimens identified as G. cichoracearum were included in the present examinations. All the re-examined collections of “G. cichoracearum” on L. communis were misidentified, and were shown to belong to Neoërysiphe nevoi or Podosphaera ergerontis-canadensis.","PeriodicalId":20165,"journal":{"name":"Phytopathologia Mediterranea","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2022-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47924901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Claudia Quartana, T. Faddetta, L. Anello, M. Di Bernardo, Rosa Petralia, V. Campanella
Five bacterial endophytic isolates obtained from durum wheat seeds (landrace “Timilia reste nere”) and identified as belonging to Pantoea (isolates A1, F7, F15 and G1) and Paenibacillus (isolate B) genera on the basis of 16S rDNA gene sequences, were assayed in vitro and in vivo for their ability to inhibit Fusarium culmorum growth and the disease (Fusarium foot rot) it causes in durum wheat. All isolates significantly reduced in vitro growth of F. culmorum in comparison with the control. After 120 hours of incubation, isolates B and G1 showed the greatest mycelial growth inhibition, i.e., respectively, 76 and 74%. When durum wheat “Simeto” seeds were treated with bacterial isolates singly or in combinations and then inoculated with F. culmorum, all treatments with endophytes showed increased, but not statistically significant, seed germination. Except for isolate A1, all bacterial isolates stimulated vegetative parameters of durum wheat seedlings. Mixture of isolates F7 + F15 was the most effective in improving shoot height (+94%), root length (+47%) and vigour index (+81%). Mixture of isolates A1 + B reduced Fusarium foot rot incidence (-21%) and severity (-30%), and isolate A1 reduced incidence (-15%) and severity (-16%) of the disease. These results indicate potential of bacterial seed endophytes, identified in this study, for control of Fusarium foot rot and suggest that bacterial seed endophytes may provide a new biocontrol agent for an environmentally sustainable durum wheat disease management programme.
{"title":"Activity of bacterial seed endophytes of landrace durum wheat for control of Fusarium foot rot","authors":"Claudia Quartana, T. Faddetta, L. Anello, M. Di Bernardo, Rosa Petralia, V. Campanella","doi":"10.36253/phyto-12993","DOIUrl":"https://doi.org/10.36253/phyto-12993","url":null,"abstract":"Five bacterial endophytic isolates obtained from durum wheat seeds (landrace “Timilia reste nere”) and identified as belonging to Pantoea (isolates A1, F7, F15 and G1) and Paenibacillus (isolate B) genera on the basis of 16S rDNA gene sequences, were assayed in vitro and in vivo for their ability to inhibit Fusarium culmorum growth and the disease (Fusarium foot rot) it causes in durum wheat. All isolates significantly reduced in vitro growth of F. culmorum in comparison with the control. After 120 hours of incubation, isolates B and G1 showed the greatest mycelial growth inhibition, i.e., respectively, 76 and 74%. When durum wheat “Simeto” seeds were treated with bacterial isolates singly or in combinations and then inoculated with F. culmorum, all treatments with endophytes showed increased, but not statistically significant, seed germination. Except for isolate A1, all bacterial isolates stimulated vegetative parameters of durum wheat seedlings. Mixture of isolates F7 + F15 was the most effective in improving shoot height (+94%), root length (+47%) and vigour index (+81%). Mixture of isolates A1 + B reduced Fusarium foot rot incidence (-21%) and severity (-30%), and isolate A1 reduced incidence (-15%) and severity (-16%) of the disease. These results indicate potential of bacterial seed endophytes, identified in this study, for control of Fusarium foot rot and suggest that bacterial seed endophytes may provide a new biocontrol agent for an environmentally sustainable durum wheat disease management programme.","PeriodicalId":20165,"journal":{"name":"Phytopathologia Mediterranea","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2022-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42389410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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